scholarly journals GERMINATION OF THREE WILDFLOWER SPECIES AFTER SEED PRIMING

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1087f-1087
Author(s):  
G. B. McClure ◽  
F. J. Sundstrom ◽  
N. S. Robbins
Keyword(s):  
Environmental Conditions ◽  
Seed Priming ◽  
Germination Percentage ◽  
Optimum Concentration ◽  
Distilled Water ◽  
Land Restoration ◽  
Industrial Sites ◽  
Coreopsis Tinctoria ◽  
Successful Establishment ◽  
Adverse Environmental Conditions

Wildflower species are being used for highway median vegetation, land restoration programs, and revegetation of industrial sites. Rapid, uniform germination is critical to establishment of new stands Seed priming techniques may increase the potential for successful establishment under adverse environmental conditions. The influence of seed priming of Gaillardia puchella, Monarda citriodora, and Coreopsis tinctoria on germination at 10° and 25° C was investigated. Seeds of the three species were placed in five levels of oxygenated KNO3 (3.5, 3.25, 3.0, 2.75, and 2.5%) solutions, or a control of oxygenated distilled water for 144 hr. Germination percentage and rate of germination (MRG) were improved for Gaillardia and Coreopsis at 10° and 25°C. Germination percentage and MRG for Monarda were not effected by priming at 25°C, but both were significantly improved at 10°C, The optimum concentration of KNO3 for greatest rapid germination was dependent on the seed variety, These results suggest that in most cases wildflower germination percentage and rates ware improved by the priming procedure.

scholarly journals GERMINATION OF THREE WILDFLOWER SPECIES AFTER SEED PRIMING

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1087F-1087
Author(s):  
G. B. McClure ◽  
F. J. Sundstrom ◽  
N. S. Robbins
Keyword(s):  
Environmental Conditions ◽  
Seed Priming ◽  
Germination Percentage ◽  
Optimum Concentration ◽  
Distilled Water ◽  
Land Restoration ◽  
Industrial Sites ◽  
Coreopsis Tinctoria ◽  
Successful Establishment ◽  
Adverse Environmental Conditions

Wildflower species are being used for highway median vegetation, land restoration programs, and revegetation of industrial sites. Rapid, uniform germination is critical to establishment of new stands Seed priming techniques may increase the potential for successful establishment under adverse environmental conditions. The influence of seed priming of Gaillardia puchella, Monarda citriodora, and Coreopsis tinctoria on germination at 10° and 25° C was investigated. Seeds of the three species were placed in five levels of oxygenated KNO3 (3.5, 3.25, 3.0, 2.75, and 2.5%) solutions, or a control of oxygenated distilled water for 144 hr. Germination percentage and rate of germination (MRG) were improved for Gaillardia and Coreopsis at 10° and 25°C. Germination percentage and MRG for Monarda were not effected by priming at 25°C, but both were significantly improved at 10°C, The optimum concentration of KNO3 for greatest rapid germination was dependent on the seed variety, These results suggest that in most cases wildflower germination percentage and rates ware improved by the priming procedure.

scholarly journals The Effects of Pre-germination Treatments on Seed Germination and Growth of Wild Guavas in the Kingdom of Eswatini, Southern Africa

2020 ◽  
pp. 28-36
Author(s):  
Thulani Sikhondze ◽  
Kwanele A. Nxumalo ◽  
Michael T. Masarirambi ◽  
Paul K. Wahome ◽  
Mathole G. Zwane
Keyword(s):  
Seed Germination ◽  
Sulphuric Acid ◽  
Growth And Development ◽  
Seed Priming ◽  
Psidium Guajava ◽  
Germination Percentage ◽  
Hot Water ◽  
Distilled Water ◽  
Ecological Zones ◽  
Poor Seed

Dormancy is a condition where seeds will not germinate even when the environmental conditions (water, temperature and aeration) are permissive for germination. Wild guavas (Psidium guajava L.) are very popular in all agro-ecological zones of Eswatini. Farmers have shown an interest towards guava cultivation but have to cope with the shortage of quality propagation material. The demand is not fulfilled because of unavailability of superior seedling rootstocks, which might be due to poor seed germination and seedling growth. Nevertheless, it has been reported that guava seeds exhibited seed dormancy, which affects their growth and development. The experiment was carried out to study effects of different pre-germination methods on seed germination of guava. The study was conducted at the University of Eswatini, Luyengo Campus. The objective of the study was to get maximum germination of guava seeds in as short a time as possible. Four methods were used i.e.,  soaking in distilled water for five days at room temperature, soaking in hot water at 80ºC for three minutes, subjecting seeds to heat at 80ºC in oven for six minutes and soaking in 20% dilute sulphuric acid for three minutes. All these treatments significantly decreased days to germination of seeds compared with the control. Among the methods, treatment of guava seeds with 20% dilute sulphuric acid for three minutes was judged best with maximum germination percentage (93.3%); lowest germination mean time was observed in seeds soaked in distilled water (31 days). Highest plant height (44 mm) and highest stem girth of (3.37 mm) were recorded from seeds soaked in sulphuric acid after 150 days of sowing. Seeds without any pre-germination treatment showed poor germination (26.7%). On the basis of the findings, it can be recommended that propagators use sulphuric acid in seed priming for higher germination, growth and development.

scholarly journals Seed Priming Technology as a Key Strategy to Increase Crop Plant Production under Adverse Environmental Conditions

2021 ◽  
Author(s):  
Daniel Garcia ◽  
Samiah Arif ◽  
Yinglei Zhao ◽  
Shuo Zhao ◽  
Lin Chau Ming ◽  
...  
Keyword(s):  
Stress Tolerance ◽  
Environmental Conditions ◽  
Seed Priming ◽  
Crop Plants ◽  
Plant Production ◽  
Food Demand ◽  
Crop Plant ◽  
Agronomic Performance ◽  
Biological Mechanism ◽  
Adverse Environmental Conditions

Farmers and seed companies constantly require high-quality seeds with excellent agronomic performance. However, faced with environmental adversity, limited natural resources and increasing food demand around the globe, more attention has turned to improving crop plant production by implementing efficient strategies. Seed priming technology has shown promising biological improvements leading to suitable agronomic performance in crop plants under adverse environmental conditions. Seeds are subjected to controlled conditions that are conducive to complex physiological, biochemical, and molecular changes, conferring specific stress tolerance to subsequent germination and growth conditions. In this review paper, we aimed to study the recent approaches in the efficiency of hydropriming, osmopriming, chemopriming, hormopriming, nanopriming, matrix priming, biopriming, physical priming and hybrid priming procedures in the production of crop plants under environmental adversity, as well as their biological mechanism changes. All priming methods demonstrated relevant changes in the biological mechanism related to crop plant production by mitigating salinity effects, heavy metals, and flooding stress and enhancing chilling, heat, drought and phytopathogen tolerance. We strongly recommend that researchers combine multiple priming methods, known as hybrid priming, in their investigations to provide novel technologies and additional biological approaches to enhance the knowledge of crop plant science. Thus, the findings shed light on the use of seed priming technology as a key strategy to increase crop plant production under environmental adversity by acquiring stress tolerance and enhancing agronomic traits to meet the global food demand.

scholarly journals Hydropriming enhances the germination of aged ultra-dry wheat seeds

2020 ◽  
Vol 48 (1) ◽  
pp. 57-63
Author(s):  
Bowen Jiang ◽  
Liyuan Wang ◽  
Chengtang Xu ◽  
Min Yan
Keyword(s):  
Hydrogen Peroxide ◽  
Amylase Activity ◽  
Soluble Sugars ◽  
Seed Priming ◽  
Germination Percentage ◽  
Oxidative Enzymes ◽  
Distilled Water ◽  
Ambient Conditions ◽  
Germination Traits ◽  
Wheat Seeds

Hydropriming is an efficient strategy for reviving aged seeds. In the present study, hydropriming was optimised for vigour enhancement in aged ultra-dry wheat seeds. Wheat seeds (initially dried to 5.0% moisture content) stored at ambient temperature for ten years were humidified over water for seven days, then soaked for 12, 24 and 36 hours in aerated distilled water (seed priming treatment). Primed and unprimed seeds were equilibrated at ambient conditions for 48 hours. Changes in germination traits, activities of anti-oxidative enzymes, hydrogen peroxide (H2O2), malondialdehyde (MDA), α-amylase activity, soluble protein and soluble sugars were determined. Results indicated that hydro priming for 12 hours significantly increased the germination percentage, from 50.7 to 58.6%. The increase in germination was significantly positively correlated with catalase activity (r = 0.969), and significantly negatively correlated with accumulation of H2O2 (r = 1.000).

scholarly journals Role of priming technique in germination parameters of calendula (Calendula officinalis L.) seeds

2016 ◽  
Vol 61 (3) ◽  
pp. 215-226
Author(s):  
Mahnaz Karimi ◽  
Masomeh Varyani
Keyword(s):  
Enzyme Activity ◽  
Soluble Sugars ◽  
Seed Priming ◽  
Germination Percentage ◽  
Distilled Water ◽  
Vigor Index ◽  
Maximum Root Length ◽  
Maximum Root ◽  
Calendula Officinalis L

Seed priming is known for its promoting effects on seed and seedling properties under stress conditions such as salinity, temperature and drought stresses. Poor plant stand establishment is a common problem for marigold production. Therefore, an experiment was conducted to investigate the effect of different seed priming techniques on calendula seed germination and seedling enzyme activity. For this purpose, different seed priming techniques: hydropriming (distilled water, 24 and 72 h), osmopriming (0.02% or 0.04% KNO3 for 24 and 72 h) and hormonal priming (100 or 500 mg L-1 GA3 for 24 and 72 h) were assessed. Seeds without any treatment were considered as control. Compared with control, 100 mg L-1 GA3 and distilled water for 72 h showed the highest promoting effects on germination percentage. The highest vigor index (525.75) was related to hydropriming (for 24 h) treatment. Also, the maximum root length was found with 0.02% KNO3 for 72 h. Catalase significantly increased in the primed seeds. The highest CAT activity and total soluble sugars were found in the seeds primed with 100 mg L-1 GA3 for 72 h. Moreover, the enzyme activity was significantly higher in the distilled water and KNO3 treatments compared with the untreated seeds.

CREATION OF THE INDUCED APPLE-THREE TETRAPLOIDS

1970 ◽  
pp. 23-25
Author(s):  
L. V. Tashmatova ◽  
О. V. Mantseva ◽  
N. V. Gorbacheva
Keyword(s):  
Environmental Conditions ◽  
Selection Process ◽  
Normal Growth ◽  
Cytological Analysis ◽  
Open Pollination ◽  
Apple Breeding ◽  
Adverse Environmental Conditions ◽  
Diploid Gametes ◽  
Resistance To Diseases

The basic moments of a process of obtaining apple tetraploids as donors of diploid gametes for apple breeding with polyploidy using are demonstrated. In industrial terms, triploids are of the greatest importance. The manifested effect of heterosis leads to the improvement of many characteristics - higher resistance to diseases, pests and adverse environmental conditions, greater autogamy than in diploids, less pronounced periodicity of fruiting, larger fruits and a convenient crown for harvesting. Triploids are developed as a result of crosses 2n × 3n or 2n × 4n. Tetraploids are necessary for more successful apple breeding with polyploidy using. For industry they are not of great importance but they are of interest as donors of diploid non-reduced gametes and allow to make the selection process more directional. One of the methods of experimental polyploidy is the induction of polyploids using mutagenes. The germs were treated with colchicines at concentrations 0.1% - 0.4% during 24 and 48 hours. According to the morphology the obtained plants were divided into five groups. Colchicine concentrations 0.3% and 0.4% during 48 hours of the treatment had a disastrous impact on the development of germs. As a result of the cytological analysis, tetraploids and chimeras were revealed, which were obtained from the seeds from the open pollination of Orlik and Svezhest (treatment variants – 0,1% colchicines solution and 24 and 48 hours of exposition), as well as from the seeds obtained as a result of the Svezhest × Bolotovskoye crossing. Tetraploids had a normal growth but they differed in large leaves, while chimeras were of low size with normal leaves and internodes.

Protection of Italian ryegrass (Lolium multiflorum L.) seedlings from salinity stress following seed priming with L-methionine and casein hydrolysate

2020 ◽  
pp. 1-9
Author(s):  
Keum-Ah Lee ◽  
Youngnam Kim ◽  
Hossein Alizadeh ◽  
David W.M. Leung
Keyword(s):  
Oxidative Stress ◽  
Amino Acids ◽  
Salinity Stress ◽  
Lolium Multiflorum ◽  
Casein Hydrolysate ◽  
Seed Priming ◽  
Germination Percentage ◽  
Italian Ryegrass ◽  
Significant Difference ◽  
Protein Amino Acids

Abstract Seed priming with water (hydropriming or HP) has been shown to be beneficial for seed germination and plant growth. However, there is little information on the effects of seed priming with amino acids and casein hydrolysate (CH) compared with HP, particularly in relation to early post-germinative seedling growth under salinity stress. In this study, Italian ryegrass seeds (Lolium multiflorum L.) were primed with 1 mM of each of the 20 protein amino acids and CH (200 mg l−1) before they were germinated in 0, 60 and 90 mM NaCl in Petri dishes for 4 d in darkness. Germination percentage (GP), radicle length (RL) and peroxidase (POD) activity in the root of 4-d-old Italian ryegrass seedlings were investigated. Generally, when the seeds were germinated in 0, 60 and 90 mM NaCl, there was no significant difference in GP of seeds among various priming treatments, except that a higher GP was observed in seeds of HP treatment compared with the non-primed seeds when incubated in 60 mM NaCl. When incubated in 60 and 90 mM NaCl, seedlings from seeds primed with L-methionine or CH exhibited greater RL (greater protection against salinity stress) and higher root POD activity than those from non-primed and hydro-primed seeds. Under salinity stress, there were higher levels of malondialdehyde (MDA) in the root of 4-d-old Italian ryegrass seedlings, a marker of oxidative stress, but seed priming with CH was effective in reducing the salinity-triggered increase in MDA content. These results suggest that priming with L-methionine or CH would be better than HP for the protection of seedling root growth under salinity stress and might be associated with enhanced antioxidative defence against salinity-induced oxidative stress.

Morphological performances of BINA soybean 6 (Glycine max) at several salinity stress concentrations in coastal region of Bangladesh

2021 ◽  
Vol 27 (02) ◽  
pp. 2287-2295
Author(s):  
F. A. Trina ◽  
R. Ahmed ◽  
R. A. Ruhi ◽  
M. I. H. Joy ◽  
M. B. J. Maliha
Keyword(s):  
Latin Square ◽  
Coastal Region ◽  
Germination Percentage ◽  
Distilled Water ◽  
Stress Concentrations ◽  
Flower Plant ◽  
Experimental Parameters ◽  
Salinity Levels ◽  
Branch Plant ◽  
Number Of Leaves

A pot experiment was conducted from 2019 to 2020 at Noakhali Science and Technology University (NSTU), Noakhali, Bangladesh, to investigate the morphological performances of BINA Soybean-6 under various saline conditions. Salinity is a widespread problem responsible for limiting the productivity of oilseed crops and soybean cultivars differ in their sensitivity to soil salinity. The research material was BINA Soybean-6 collected from the Bangladesh Institute of Nuclear Agriculture (BINA), Mymensingh. There were five treatments of salinity viz., T0 (distilled water), T1 (50mMNaCl), T2 (100mMNaCl), T3 (150mMNaCl) and T4 (200mMNaCl) were used in the experiment in which distilled water was used as control. Latin Square Design (LSD) with three replications was used in this experiment. In the present study, BINA Soybean 6 showed a significant variation in the contributing parameters. The experimental parameters used during the experiment were germination percentage and duration, height of plants (cm), number of branches/plant, number of leaves/plant, number of flowers/plant, number of pods/plant, length of pods (cm), seeds/pod, and length of root (cm). The highest germination percentage (83.33%) was observed in control (T0) condition while germination percentage was suppressed under T4 treatment i.e. 200mMNaCl concentration. Germination percentages were gradually decreased with the increase of salinity level. The highest plant height (34.22 cm), number of branch plant-1 (3.44), leaf number plant-1 (13.56), number of flower plant-1 (24.33), pod number plant-1 (21.22), seeds pod-1 (3.33), length of pod (5.44 cm) and root length (15.89 cm) were obtained at control (T0) condition. It was observed that the parameters were diminished gradually with the rising of salinity levels compared to the treatment T0 (Control).

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