scholarly journals Mycoflora impact on germination and vigour of bottle gourd (Lagenaria siceraria) seeds and its management through different plant protectants

2021 ◽  
Author(s):  
Namita Soni ◽  
Kushal Raj ◽  
V. S. Mor ◽  
Rakesh Kumar Chugh
Keyword(s):  
Germination Percentage ◽  
Fusarium Proliferatum ◽  
Bottle Gourd ◽  
Copper Oxychloride ◽  
Germination Inhibition ◽  
Seedling Vigour ◽  
Vigour Index ◽  
The Impact ◽  
Highly Virulent

Bottle gourd is an important cucurbitaceous crop. The impact of 14 species from eight fungal genera on bottle gourd seeds was studied through in vitro tests. Bottle gourd seeds were inoculated with each isolate separately and subjected to various germination tests. Maximum germination inhibition was upto 69.3% due to Fusarium proliferatum. Inoculation of seeds with F. proliferatum significantly reduced the germination speed index to 2.69. The seedling vigour index I for seeds inoculated with F. proliferatum and F. incarnation-equiseti (species complex) was 860 and 861, respectively, significantly different from that of non-inoculated seeds having a vigour index of 1791. The seedling vigour index II of seeds inoculated with F. proliferatum was 1818, significantly lower than the control (4631), indicating that the F. proliferatum isolate was highly virulent among all the isolates. Amongst seven plant protectants evaluated for their efficacy as seed dressers against F. proliferatum, propioconazole (25% EC), copper oxychloride (50% WP) and Mancozeb (75% WP) were effective in reducing infection. Germination percentage was highest for seeds treated with the bioagent Trichoderma harzianum 1% WP.

In Vitro Investigation of Biopriming on Seed Quality Parameters in Green Gram [Vigna radiata (L.)]

2019 ◽  
Author(s):  
Ashok S Sajjan ◽  
Sujaya Waddinakatti ◽  
R B Jolli ◽  
Geetha D Goudar
Keyword(s):  
Rhizobium Leguminosarum ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Fluorescent Pseudomonad ◽  
Seedling Vigour ◽  
In Vitro Investigation ◽  
Vigour Index

The in vitro investigation was conducted during the kharif 2017 in the Department of Seed Science and Technology, College of Agriculture, Vijayapura. The experiment comprised of nine biopriming treatments along with control. The experiment was laid out in CRD with three replications. The seeds were subjected to in vitro studies and data was analysed statistically. The seed bioprimed with Rhizobium leguminosarum @ 20 per cent recorded higher seed germination percentage (90.50 %), shoot length (19.27 cm), root length (12.38 cm), seedling length (31.65 cm), seedling dry weight (373.0 mg), seedling vigour index I (2864), seedling vigour index II (33.75), dehydrogenase enzyme activity (1.010 OD value) and lower electrical conductivity (0.107 dS m-1) followed by Pseudomonas fluorescens @ 20 per cent, Fluorescent pseudomonad sp. @ 20 per cent as compared to control.

scholarly journals Evaluation of Fungicides and Bioagents against Fusarium proliferatum under In vitro by Spore Germination Inhibition Technique

2021 ◽  
Author(s):  
Namita Soni ◽  
Kushal Raj ◽  
S. Vijaykumar
Keyword(s):  
Seed Germination ◽  
Pseudomonas Fluorescens ◽  
Spore Germination ◽  
Integrated Approach ◽  
Fusarium Proliferatum ◽  
Germination Inhibition ◽  
Seedling Vigour ◽  
Tropical Regions ◽  
Inhibition Technique

Background: Bottle gourd is a cucurbitaceous vegetable of culinary and medicinal importance cultivated in various tropical and sub-tropical regions of world. This crop is exposed to a wide variety of seed and soil mycoflora, out of which Fusarium proliferatum is utmost important as far as seed germination, viability and seedling vigour are concerned. Methods: Study was taken up to evaluate different fungicides and bioagents for their efficacy against the fungus Fusarium proliferatum under in vitro through spore germination inhibition technique. Result: Spore germination inhibition of 86.00%, 85.00% and 81.33% was recorded with hexaconazole (5% SC) @ 0.2% (C3), mancozeb (75% WP) @ 0.3% (C3) and Pseudomonas fluorescens (1% WP) @ 2% (C3), respectively. The inhibition in spore germination by mancozeb (75% WP) and Pseudomonas fluorescens (1% WP) was upto 77.33% and it was 61.78% and 67.33% in treatments involving carbendazim (50% WP) and Trichoderma harzianum (1% WP) that could be exploited to devise integrated approach for disease management.

scholarly journals Development of priming technology for enhanced planting value of seeds in kabuli chickpea (Cicer arietinum L.)

2021 ◽  
Vol 13 (2) ◽  
pp. 735-743
Author(s):  
Umesh Hiremath ◽  
Basave Gowda ◽  
Lokesh G.Y . ◽  
B. S. Ganiger
Keyword(s):  
Root Length ◽  
Seed Quality ◽  
Dry Weight ◽  
Germination Percentage ◽  
Quality Parameters ◽  
Shoot Length ◽  
Growth And Yield ◽  
Seedling Vigour ◽  
Seedling Length ◽  
Vigour Index

The continuous rise in global population demands more food production under limited land resources accompanied by climate change. More oversupply of quality seeds to meet ever-growing food demand is a highly challenging task. Seeds with low vigour level limit the growth and yield of crops at varied field conditions. Hence, an experiment was conducted to develop a technique for the fast and homogeneous growth of kabuli chickpea seeds. The priming treatments evaluated in the present study enhanced the various seed quality parameters of kabuli chickpea significantly when compared to control. Hydro-priming for 4 hours (at 20°C) and air drying for 72 hours showed better results compared to rest of the treatments both in fresh seeds which recorded higher first count (66.38%), germination (92.50%) root length (11.85 cm), shoot length (6.16), mean seedling length (18.01), dry seedling weight (747 mg), seedling vigour index-I (1665), higher speed of emergence (0.3992) and old seeds with higher first count (61.88%), germination (74.13%) root length (8.15 cm), shoot length (5.85 cm), mean seedling length (14.00 cm), seedling dry weight (611 mg), seedling vigour index-I (1056), higher speed of emergence (0.3814). The aged seeds have recorded 29 % increase in germination percentage in contrast to 8 percent increases in fresh seeds. Kabuli chickpea seeds could be subjected to hydropriming, an affordable, alternative, and eco-friendly technique for improving seed and seedling vigour of kabuli chickpea.

Effect of seeding date on canola seed quality and seed vigour

2004 ◽  
Vol 84 (2) ◽  
pp. 463-471 ◽  
Author(s):  
L. V. Gusta ◽  
E. N. Johnson ◽  
N. T. Nesbitt ◽  
K. J. Kirkland
Keyword(s):  
Brassica Napus ◽  
Seed Quality ◽  
Seedling Emergence ◽  
Germination Percentage ◽  
Canola Seed ◽  
Higher Plant ◽  
Seedling Vigour ◽  
Seed Vigour ◽  
Seeding Date ◽  
The Impact

Seedling vigour is a key critical component to produce well-established canola (Brassica napus) seedlings under less than ideal conditions in western Canada. Our objective was to determine if seed lots from late October (fall) or early spring (April) seeding dates improve seed vigour relative to mid-May (May) seeding dates. We compared seed size, seed maturity, germination percentage, and seedling emergence under controlled-environment conditions from six seed lots obtained from fall-sown (1997, 1998), April-sown (1998, 1999) and May-sown canola (1998, 1999) cv. Quest. On average, 80% of fall-derived seed for both years was larger than 1.7 mm compared to only 32% for May-derived canola seed. April-derived seed was intermediate. Fall and April derived seed were considered 40% mature compared to 16% for May-derived seed. In germination tests at 8°C, May derived seed germinated and emerged slower with a lower total germination percentage compared to falland April-derived seed lots. Priming reduced differences in emergence between the seed lots. Field studies at Scott, SK, sown in the fall of 1999 and 2000, and in early May of 2000 demonstrated that seed derived from fall- and April-sown canola produced higher plant densities, higher biomass at bolting, and higher seed yield than seed derived from May-sown canola. Yield was 10 to 45% less from seed derived from May-sown canola than seed derived from fall- and April-sown canola. This study established the impact of seeding date on seed quality and vigour, which in turn affected emergence, seedling vigour and yield. Also, seed vigour slowly declined within 1 yr, primarily with seed derived from the May-sown canola. Key words: Canola, Brassica napus, vigour (seed), seeding (fall), emergence, yield (quality)

scholarly journals Cryptococcus gattii Infection Dampens Th1 and Th17 Responses by Attenuating Dendritic Cell Function and Pulmonary Chemokine Expression in the Immunocompetent Hosts

2014 ◽  
Vol 82 (9) ◽  
pp. 3880-3890 ◽  
Author(s):  
Pornpimon Angkasekwinai ◽  
Nuntarat Sringkarin ◽  
Oratai Supasorn ◽  
Madtika Fungkrajai ◽  
Yui-Hsi Wang ◽  
...  
Keyword(s):  
Immune Responses ◽  
Cell Function ◽  
Cryptococcus Gattii ◽  
Cell Immune Response ◽  
Content Type ◽  
Chemokine Expression ◽  
Immunocompromised Hosts ◽  
The Impact ◽  
Highly Virulent

ABSTRACTCryptococcal infections are primarily caused by two related fungal species:Cryptococcus neoformansandCryptococcus gattii. It is well known thatC. neoformansgenerally affects immunocompromised hosts; however,C. gattiiinfection can cause diseases in not only immunocompromised hosts but also immunocompetent individuals. While recent studies suggest thatC. gattiiinfection could dampen pulmonary neutrophil recruitment and inflammatory cytokine production in immunocompetent hosts, the impact ofC. gattiiinfection on the development of their adaptive T helper cell immune response has not been addressed. Here, we report thatC. neoformansinfection with highly virulent and less virulent strains preferentially induced pulmonary Th1 and Th17 immune responses in the host, respectively. However, fewer pulmonary Th1 and Th17 cells could be detected in mice infected withC. gattiistrains. Notably, dendritic cells (DC) in mice infected withC. gattiiexpressed much lower levels of surface MHC-II andIl12orIl23transcripts and failed to induce effective Th1 and Th17 differentiationin vitro. Furthermore, the expression levels ofIp10andCxcl9transcripts, encoding Th1-attracting chemokines, were significantly reduced in the lungs of mice infected with the highly virulentC. gattiistrain. Thus, our data suggest thatC. gattiiinfection dampens the DC-mediated effective Th1/Th17 immune responses and downregulates the pulmonary chemokine expression, thus resulting in the inability to mount protective immunity in immunocompetent hosts.

scholarly journals Impact of tannery effluent on Sorghum bicolor (L.) Moench

2021 ◽  
pp. 146-149
Author(s):  
V. Sangeetha ◽  
P.S. Sharavannan
Keyword(s):  
Sorghum Bicolor ◽  
Germination Percentage ◽  
Shoot Length ◽  
Tolerance Index ◽  
Tannery Effluent ◽  
Randomized Design ◽  
Vigour Index ◽  
Completely Randomized Design ◽  
The Impact ◽  
Sorghum Bicolor L

The present study is aimed to investigate the effect of tannery effluent on different varieties of Sorghum bicolor. The disparate concentrations of tannery effluent viz., 5, 25, 50, 75 and 100% were tested for its impacts on six varieties of Sorghum bicolor (L.) Moench (TNAU CO 5, TNAU CO 30, CO (S) 28, BSR 1, K Tall and Paiyur 1). The experimentation was carried out in a completely randomized design (CRD) with five replications. The impact of tannery effluent on the germination percentage, shoot length (cm), root length (cm), seedling weight (g), vigour index, tolerance index and phytotoxicity were recorded. The outcome revealed that the parameters escalated in 5% effluent concentration and declined after 25% concentration of tannery effluent.

Influence of Priming with Exogenous Selenium on Seed Vigour of Alfalfa (Medicago sativa L.)

2021 ◽  
Author(s):  
F.S. Xia ◽  
C.C. Wang ◽  
Y.Y. Li ◽  
Y.Y. Yang ◽  
C. Zheng ◽  
...  
Keyword(s):  
Higher Plants ◽  
Seed Priming ◽  
Germination Percentage ◽  
Health Concern ◽  
Global Public Health ◽  
Seedling Vigour ◽  
Seed Vigour ◽  
Vigour Index ◽  
Low Selenium ◽  
Alfalfa Seeds

Background: Selenium (Se) is an essential trace element to higher plants, animals and humans, but low Se levels are a global public health concern. Seed priming has become a basic strategy for the production of Se-riched agricultural products, but its application is still not clear in the production of Se-enrich alfalfa, hence this study was conducted for the production of Se-enriched alfalfa by seed priming with different concentrations and time on seed vigour. Methods: Seeds were primed with 0, 0.5, 1.0, 2.0, 4.0 and 8.0 mmol L-1 of sodium selenite solution for 0, 3, 6, 9 and 12 h at 20°C and their germination percentage, germination index, seedling vigour index and mean germination time were analyzed. Result: Seed vigour of alfalfa was improved by priming with low selenium (Se) concentration (0.5 and 1.0 mmol L-1), but was inhibited by high Se concentration ( greater than 2.0 mmol L-1). Hence, it must be necessary to carefully select appropriate concentration and time for the application of Se priming in alfalfa seeds. The optimal manner of Se priming in alfalfa seeds might be at 1.0 mmol L-1 concentration for 9 h.

Impact of Seed Maturation and Drought on Seed Germination and Early Seedling Growth in White Clover (Trifolium repens L.)

2021 ◽  
Author(s):  
Ruqiang Tong ◽  
Xinyuan Liu ◽  
Bifan Mu ◽  
Junfeng Wang ◽  
Mengxing Liu ◽  
...  
Keyword(s):  
Seedling Growth ◽  
White Clover ◽  
Germination Percentage ◽  
Seed Maturation ◽  
Percentage Germination ◽  
Seedling Vigour ◽  
Early Seedling Growth ◽  
Vigour Index ◽  
Early Seedling ◽  
The Mean

Background: Understanding the drought tolerance of white clover seeds during germination and early seedling growth and how that is linked to seed maturation in legume species, is crucial to developing new stand establishments.Methods: An experiment was conducted in Randomized Block design in two factorial concept to explore how seed maturation and drought tolerance influence seed germination and early seedling growth using the two factors: seed coat color (yellow and brown, two levels as one factor) and drought intensity (control: 0 MPa [distilled water], mild drought: -0.2 MPa, moderate drought: -0.4 MPa and severe drought: -0.6 MPa, four levels as another factor).Result: In contrast to the yellow-coated seeds, the mean germination percentage, germination index, seedling vigour index and radicle length of the brown-coated seeds were lowered by 7.9%, 23.1%, 20.5% and 12.9%, respectively. Compared to the control, introducing drought conditions reduced the mean of germination percentage, germination index, seedling vigour index and radicle length of yellow-coated seeds by 52.4%, 64.1%, 41.9% and 23.2%, respectively. Meanwhile, drought affected brown-coated seeds more than yellow-coated seeds. The results indicating that the seed quality of white clover seed lots with yellow-colored coats higher than the seed lots with brown-coloured coats. In agronomic practice, a larger proportion of yellow-coated seeds should be harvested to improve new stands successful establishment under environment easy to dry.

scholarly journals An improved method of seed germination testing in Kabuli chickpea

2018 ◽  
Author(s):  
Basave Gowda ◽  
A. Krishna Naik ◽  
Rakesh . ◽  
C. Mathad ◽  
B.S. Ganiger ◽  
...  
Keyword(s):  
Seed Germination ◽  
Germination Percentage ◽  
Improved Method ◽  
Testing Methods ◽  
Seedling Vigour ◽  
Seed Testing ◽  
Testing Method ◽  
Vigour Index ◽  
Paper Method ◽  
Germinated Seeds

An experiment was conducted during 2014-2015 to standardize the seed testing method in Kabuli chickpea MNK-1 by number of seeds and number of germination papers. Among the different testing methods 25 seeds per replication recorded highest seed germination percentage (91.58%), less number of fresh un-germinated seeds (1.91%), abnormal seedlings (4.00%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2215 and 6863), among the number of germination papers used 2+1 as in between paper method(Bottom 2 and one paper above) recorded significantly higher germination percentage (92.12%), less number of fresh un-germinated seeds (1.41%), abnormal seedlings (3.66%), diseased seeds (2.33%) and significantly higher seedling vigour index-I and II (2463 and 6900), when compared to other. Irrespective of number of germination paper and seeds used however, interaction of 25 seeds per replication and 2+1 between paper method of seed germination testing showed significantly germination percentage (96.50%), less number of fresh un-germinated seeds (0.00%), abnormal seedlings (1.00%), diseased seeds (1.00%) and significantly higher seedling vigour index-I and II (2844 and 7792) followed by 50 seeds per replication. 

scholarly journals Quality of Vegetable Seeds Collected from Mymensingh Region in Bangladesh

2014 ◽  
Vol 2 (1) ◽  
pp. 103-108
Author(s):  
I. Hossain ◽  
P. Dey ◽  
K. Dilruba
Keyword(s):  
Seed Quality ◽  
Germination Percentage ◽  
Bottle Gourd ◽  
Amaranthus Tricolor ◽  
Seedling Vigour ◽  
Basella Alba ◽  
Brassica Oleraceae ◽  
Total Seed ◽  
Penicillium Spp

Seed quality and health status of 11 vegetable crop seeds of viz. Cabbage (Brassica oleraceae var. capitata), Indian cabbage (Brassica oleraceae var. indica), Indian spinach (Basella alba), Spinach (Beta vulgaris var. bengalensis), Red amaranth (Amaranthus tricolor), Bitter gourd (Momordic acharantia), Bottle gourd (Lagenaria siceraria), Sweet gourd (Cucurbita moschata), Carrot (Daucuscarrota var. sativa),  Radish (Raphanus sativus), and Turnip (Brassica rapa) collected from the greater Mymensingh region of Bangladesh were tested. The germination percentage of seeds of the collected samples ranged from 11 to 100. The highest germination was recorded in indian cabbage (100%) followed by carrot (92%) and radish (90%), while the lowest was recorded in indian spinach seeds (11%). Altogether 10 fungi were found associated with the seeds which were Alternaria spp., Aspergillus flavus, Aspergillus niger, Botrytis cinerea, Chaetomium funicola, Curvularia spp., Fusarium spp., Penicillium spp., Phoma spp. and Rhizopus spp.. The highest total seed-borne fungal infection was found in bottle gourd (155%) followed by sweet gourd (145%). The lowest infection was found in turnip seeds (6%). The maximum number of dead seeds was found in indian spinach (89%) and no dead seed was found in indian cabbage. Among the vegetables seed samples, seedling vigour ranged from 59 to 3083, where the highest seedling vigour was observed in sweet gourd (3083) and the lowest was in indian spinach (59). DOI: http://dx.doi.org/10.3126/ijasbt.v2i1.9926 Int J Appl Sci Biotechnol, Vol. 2(1): 103-108

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