Effect of BAP (6-Benzyl Amino Purine) Concentration  on Growth Micro Cutting of Nepenthes ampullaria

Conventionally, cultivations of Nepenthes are conducted by using seeds, cutting, and filial separation. However, there are many obstacles come both from time and technical aspect. In vitro culture is an alternative way for cultivating N. ampullaria (Jack,). One of a technique of in vitro culture is micro cutting. BAP (6-benzyl amino purine) growth regulator could be added to optimize the growth of N. ampullaria microcutting. The purpose of this research was to determine the effect of BAP on the growth of Nepenthes microcutting. This research was done experimentally using Completely Randomized Design (CRD). BAP treatment consisted of 5 concentrations: 0; 0.5; 1; 1.5; 2 (ppm), each treatment were multiplied 4 times. The parameters observed were: a time of bud initiation, time of root initiation, total of leaves, total of new buds, total of roots, length of leave, length of root, and height of bud. The data obtained were analyzed with ANOVA (Analisis of Variance) and continued with 5% and 1% LSD (Least Significant Different) test. The result showed that addition of BAP affected the growth of N. ampullaria microcutting in total leaves, length of leave, and total of buds. LSD test proved that 0.57 ppm of BAP was optimal concentration to increase total buds, whit the value reached of 3.86. Here, we found that BAP can be utilized to enhanche N. ampullaria growth on in vitro culture. The benefit of this study is to conserve N. ampullaria in vitro using BAP at 0.57 ppm.

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INDUKSI DAN REGENERASI KALUS KELADI TIKUS (Typonium flagelliforme. Lodd. ) SECARA IN VITRO

Jurnal Penelitian Tanaman Industri ◽

10.21082/jlittri.v13n4.2007.142-146 ◽

2020 ◽

Vol 13 (4) ◽

pp. 142 ◽

Cited By ~ 2

Author(s):

SITTI FATIMAH SYAHID ◽

NATALINI NOVA KRISTINA

Keyword(s):

Tissue Culture ◽

Genetic Variation ◽

Carbon Source ◽

Genetic Variability ◽

In Vitro Culture ◽

Basic Medium ◽

Randomized Design ◽

Completely Randomized Design ◽

Group B

ABSTRAK Keladi tikus umumnya diperbanyak secara vegetatif sehingga ragam genetiknya sempit. Penelitian peningkatan keragaman genetik pada keladi tikus melalui kultur in vitro telah dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Obat dan Aromatik (Balittro) Bogor pada bulan April sampai Desember 2005. Bahan tanaman yang digunakan adalah daun steril keladi tikus in vitro. Media dasar yang digunakan adalah Murashige and Skoog (MS) yang diperkaya vitamin dari group B. Sebagai sumber energi digunakan sukrosa sebanyak 30 g/l. Penelitian terdiri dari dua tahap yaitu induksi dan regenerasi kalus. Perlakuan yang diuji pada tahap I adalah beberapa taraf konsentrasi auksin (2,4-D) secara tunggal maupun kombinasi dengan sitokinin (kinetin) terhadap induksi kalus yaitu : 2,4-D 0,1 mg/l; 2,4-D 0,5 mg/l; 2,4-D 1,0 mg/l; 2,4-D 0,1 + kinetin 0,1 mg/l; 2,4-D 0,5 mg/l + kinetin 0,1 mg/l; 2,4-D 1.0 mg/l + kinetin 0,1 mg/l; 2,4-D 0,1 mg/l + kinetin 0,3 mg/l; 2,4-D 0,5 mg/l +kinetin 0,3 mg/l dan 2,4-D 1,0 mg/l + kinetin 0,3 mg/l. Tahap II adalah beberapa taraf konsentrasi benzyl adenin untuk regenerasi kalus. Penelitian disusun menggunakan rancangan acak lengkap dengan pola faktorial dan lima ulangan, dan setiap ulangan terdiri dari satu eksplan. Faktor pertama adalah asal kalus dan faktor kedua adalah beberapa taraf konsentrasi BA yaitu : BA 0,1 mg/l ; BA 0,3 mg/l dan BA 0,5 mg/l. Parameter yang diamati adalah waktu inisiasi kalus, struktur dan warna kalus, jumlah tunas serta penampilan kultur secara visual. Hasil penelitian menunjukkan bahwa kalus asal eksplan daun dapat diinduksi pada perlakuan 2,4-D 1,0 mg/l + kinetin 0,1 mg/l dan 2,4-D 1,0 mg/l + kinetin 0,3 mg/l dengan waktu inisiasi 8 sampai 10 minggu setelah perlakuan. Regenerasi kalus terbaik diperoleh pada medium 2,4-D 1,0 mg/l + kinetin 0,3 mg/l mengandung BA 0,3 mg/l dengan rata-rata tunas dan daun yang dihasilkan sebanyak 13,2 tunas dan 4,4 daun. Kata kunci : Keladi tikus, Typonium flagelliforme Lodd., induksi, regenerasi kalus, in vitro ABSTRACT Induction and regeneration of Rodent tuber calli through in vitro culture Rodent tuber plant (Typonium flagelliforme Lodd) is commonly propagated vegetatively, the repro its genetic variation is narrow. A research to increase the genetic variability of the plant was conducted in Tissue Culture Laboratory of the Indonesian Medicinal and Aromatic Research Institute, Bogor from April to December 2005. The leaf of Rodent tuber in vitro used as an explants. Murashige and Skoog (MS) medium used as basic medium, supplemented with vitamin from B group, sucrose 30 g/l was added into the medium as carbon source. The research consist of two steps : 1) calli induction and 2) calli regeneration. The treatment tested in first step : 2.4-D 0.1 mg/l; 2.4-D 0.5 mg/l; 2.4-D 1,0 mg/l; 2.4-D 0.1 + kinetin 0.1 mg/l; 2.4-D 0.5 mg/l + kinetin 0.1 mg/l; 2.4- D 1.0 mg/l + kinetin 0,1 mg/l; 2.4-D 0.1 mg/l + kinetin 0.3 mg/l; 2.4-D 0.5 mg/l + kinetin 0.3 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l. In the second steps, several concentration of BA were tested i.e: BA 0,1 mg/l ; BA 0,3 mg/l and BA 0,5 mg/l. The experiment was arranged in completely randomized design with factorial pattern. Each treatment consist of five replications. The parameters observed were time of calli initiation, texture, colour of calli and number of shoot and leaves in regeneration. The result showed that calli can be induced on 2.4-D 1.0 mg/l + kinetin 0.1 mg/l and 2.4-D 1.0 mg/l + kinetin 0.3 mg/l, eight to ten weeks after culture. The best medium for shoots regeneration contains 2.4- D 1.0 mg/l + kinetin 0.3 mg/l with 0.3 mg/l BA, with mean result of 13.2 shoots and 4.4 leaves. Key words : Rodent tuber, Typonium flagelliforme Lodd. bl , induction, regeneration, calli, in vitro

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INDUKSI AKAR JERUK SIAM (Citrus nobilis Lour.) ASAL KAMPAR DARI TUNAS IN VITRO PADA MEDIA ½ MS DENGAN PENAMBAHAN IBA DAN NAA

JURNAL BIOLOGI UNAND ◽

10.25077/jbioua.8.1.29-35.2020 ◽

2020 ◽

Vol 8 (1) ◽

pp. 29

Author(s):

Rasyidah Ulfa

Keyword(s):

In Vitro Culture ◽

Randomized Design ◽

Fruit Skin ◽

Completely Randomized Design

Orange is very popular as a fruit of consumption in Indonesia, including Riau. One type of citrus which is the mainstay of commodity cultivation in Riau province is siam orange (Citrus nobilis Lour.). Siam orange has a sweet, fragrant aroma and thin fruit skin. Siam orange cultivation needs improvement for having the best quality plants. One effort that can do is in vitro culture. The study aimed to determine the best IBA and NAA concentrations to trigger the formation of siam orange (Citrus nobilis Lour.) roots. This study using a Completely Randomized Design (CRD). The result of this study shows that the rooting percentage obtained 100% on all treatments. The fastest rooting obtained in 2 mg/L IBA treatment (7,67 days after planting). The highest number of roots obtained in the combination of 0.5 mg/L NAA with 1.5 mg/L IBA (2.67 units). The longest root obtained in the combination of 1.5 mg/L NAA with 0.5 mg/L IBA (2.27 cm).

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Production of reserpine of rauwolfia serpentina [L] kurz ex benth through in vitro culture enriched with plant growth regulators of NAA and kinetin

International Journal of Engineering & Technology ◽

10.14419/ijet.v7i2.29.13331 ◽

2018 ◽

Vol 7 (2.29) ◽

pp. 274 ◽

Cited By ~ 1

Author(s):

Muhammad Subandi ◽

Dikayan . ◽

Efrin Firmansyah

Keyword(s):

In Vitro Culture ◽

Growth Regulators ◽

High Performance ◽

Culture Method ◽

Naphthalene Acetic Acid ◽

Randomized Design ◽

Completely Randomized Design ◽

Institute Of Technology ◽

Design Weight

Rauwolfia serpentine [L] Kurz ex Benth is a valuable plant yielding alkaloid of reserpine. The aim of this study was to find the most effective way of reserpine production. Growth regulators {naphthalene acetic acid [NAA] and Kinetin}were applied to promote and to regulate the growth of explant of R. serpentine cultured in vitro. The study was conducted at the laboratory of Bandung Institute of Technology [ITB]. Nine formulations of NAA and Kinetin were the treatments that were repeated twice in completely randomized design. Weight of callus was measured at one, two, three and four weeks after induction of explant. Reserpine analysis was performed by High Performance Liquid Chromatography [HPLC]. The result showed that the best callus induction was the treatment of 2.5 ppm NAA + 2.5 ppm Kinetin, and the highest content of reserpine was in root organ [0.0021 g / L], and in callus [0.0021 g / L] at the age of 4 weeks after induction. There was revealed that in vitro culture method was more productive in producing reserpine compound than the conventional plantation of R serpentine. Production of reserpine by callus culture was more effective and may be the basic for recommended further effort.

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MULTIPLIKASI Aquilaria malaccensisDENGAN NAA DAN RAGI PADA KULTUR IN VITRO

Jurnal Pemuliaan Tanaman Hutan ◽

10.20886/jpth.2021.15.1.51-59 ◽

2021 ◽

Vol 15 (1) ◽

pp. 51-59

Author(s):

Samanhudi Samanhudi ◽

◽

Amalia Tetrani Sakya ◽

Indah Tri Retnosari

Keyword(s):

Plant Physiology ◽

In Vitro Culture ◽

Yeast Extract ◽

Yeast Growth ◽

Randomized Design ◽

Aquilaria Malaccensis ◽

Number Of Leaves ◽

Completely Randomized Design ◽

Yeast Concentration

Multiplication of Aquilaria malaccensis with naa and yeast growth regulators on in vitro culture. This study aims to obtain the best concentration of NAA and yeast extract for multiplication of agarwood on in vitro culture. This research was conducted from January to October 2020 at the Laboratory of Plant Physiology and Biotechnology, Faculty of Agriculture, Universitas SebelasMaret, Surakarta. The experimental design used was a factorial completely randomized design (CRD) with twofactors, namely NAA (0 ppm; 0.1 ppm; 0.2 ppm; and 0.3 ppm) and yeast extract (0 mg/l, 700 mg/l, 800 mg/l, and 900 mg/l).(+)The results showed that the combination of 0 ppm NAA and 900 mg/lyeast increasedthe number of shoots of A.malaccensis explants with the highest average number of 3.67 shoots. A single NAA concentration of 0 ppm was able to increase the number of leaves of explants of A. malaccensis with the highest average leaf rate of 24.5 leaves. A single yeast concentration of 0 mg/lwas able to increase the number of leaves of A. malaccensis explants with an average of 22 leaves.

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MULTIPLIKASI TUNAS PUCUK TOMAT (Lycopersicum esculentum MILL) DENGAN MENGGUNAKAN BENZYL AMINO PURINE (BAP) DAN NAPHTALENE ACETIC ACID (NAA) SECARA IN VITRO

10.31219/osf.io/j75gd ◽

2019 ◽

Author(s):

F. Fathurrahman

Keyword(s):

Acetic Acid ◽

Shoot Multiplication ◽

Shoot Height ◽

Factor B ◽

Randomized Design ◽

Two Factors ◽

Completely Randomized Design ◽

Benzyl Amino Purine ◽

Number Of Shoots

Research with the title shoot multiplication shoots tomatoes (Lycopersicumesculentum mill) using the Benzyl Amino Purine (BAP) dan Naphtalene Acetic Acid(NAA) in vitro have been conducted at the Laboratory of Biotechnology Faculty ofAgriculture, Islamic University of Riau, Pekanbaru. This research has been carried outfor three months carried out startingfrom November 2010 to February 2011. This studyaims to determine the effect of a single interaction between the administration and plantgrowth regulators BAP and NAA on shoot multiplication of in tomato shoots vitro.Rancangan used in this study was completely randomized design (CRD) in factorialwhich consist of two factors. The first factor is factor B (concentration of BAP) withfour standard treatments are: B0 (0 ppm), B1 (1 ppm), B2 (2 ppm), B3 (3 ppm).Thesecond factor is the factor (the concentration of NAA) with four standard treatments,including: N0 (0 ppm), N1 (0.1 ppm), N2 (0.5 ppm), and N3 (1 ppm), to obtain 16combined treatment with three replications. Parameters observed, namely: age emergedshoots, number of shoots, shoot height, the percentage of growing shoots, roots andgrowing percentage of the number of explants forming callus. The data was statisticallyanalyzed the results of observations, when the F calculated is greater than the F table,followed by a further test of honest real difference (HRD) 5%. From the results ofresearch in the interaction of BAP and NAA effect on the parameters of high-shoots bytreatment tebaik B1N0 namely 6.16 cm. BAP singly significantly affect the parametersage appears buds (days) and the percentage grows shoots with the best treatment B2 (2ppm), shoot height with the best treatment B0 (0 ppm), the number of shoots (the fruit)with the best treatment B3 (3 ppm). singly whereas NAA significantly affect theparameters age appears shoots (day) and high-shoots with the best treatment N0 (0ppm), and the percentage grows roots with the best treatment N3 (1 ppm).

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MULTIPLICATION EXAMINATION OF TURMERIC EXPLANTS USING AUXIN AND CYTOKININ TO MODIFIED MEDIA

Jurnal Penelitian Saintek ◽

10.21831/jps.v24i1.19503 ◽

2019 ◽

Vol 24 (1) ◽

pp. 39-45

Author(s):

Nur Azizah Romadhoni ◽

Erni Suminar ◽

Anne Nuraini ◽

Syariful Mubarok

Keyword(s):

Tween 80 ◽

Seed Technology ◽

Randomized Design ◽

Explant Source ◽

Murashige Skoog ◽

Completely Randomized Design ◽

Materials Used ◽

Modified Media ◽

Benzyl Amino Purine

This study was aimed at obtaining the type and concentration of cytokines as well as the optimal concentration of auxin for the multiplication of turmeric shoots in vitro. The trial was conducted in September 2017 until February 2018 at the Tissue Culture Seed Technology Laboratory of the Faculty of Agriculture, Padjadjaran University. The materials used in this study were Murashige and skoog modified multiplication medium, jelly, sterile distilled water, HgCl2, 70% alcohol, clorox, tween 80 and fungicide and bactericidal. Growth Regulating Substances (GRS) used are BAP, TDZ, Zeatin and NAA. The explant source used was derived from the shoot of turmeric clones from Bogor. The experimental design in this study was Completely Randomized Design (CRD) with seven treatments and four replications. The planting medium used was Murashige Skoog Modification (MS) with the addition of Benzyl Amino Purine (BAP) 9 mg L-1, Thidiazuron (TDZ) 1 mg L-1, Zeatin 0.1 mg L-1 and NAA (0.01 mg L-1, 1 mg L-1). The results show that the combination of BAP 9 mg L-1 and NAA 0.01 mg L-1 produced the highest shoot induction at 12 weeks after planting. Giving zeatin 0.1 mg L-1 and NAA 1 mg L-1 produces the highest shoot length at 12 weeks after planting.PENGUJIAN MULTIPLIKASI EKSPLAN KUNYIT DENGAN PENAMBAHAN AUKSIN DAN SITOKININ PADA MODIFIKASI MEDIAPenelitian ini bertujuan untuk mendapatkan jenis dan konsentrasi sitokinin serta konsentrasi auksin yang optimal untuk multiplikasi tunas kunyit secara in vitro. Percobaan dilaksanakan pada bulan September 2017 sampai Februari 2018 di laboratorium Kultur Jaringan Teknologi Benih Fakultas Pertanian Universitas Padjadjaran. Bahan yang digunakan dalam penelitian ini adalah murashige dan skoog modified multiplication medium, agar-agar, aquades steril, HgCl2, alkohol 70%, clorox, tween 80, fungisida, dan bakterisida. Zat Pengatur Tumbuh (ZPT) yang digunakan yaitu BAP, TDZ, Zeatin, dan NAA. Sumber eksplan yang digunakan yaitu berasal dari tunas kunyit klon asal Bogor. Rancangan percobaan pada penelitian ini yaitu Rancangan Acak Lengkap (RAL) dengan tujuh perlakuan dan empat ulangan. Media tanam yang digunakan yaitu Murashige Skoog Modifikasi (MS) dengan penambahan Benzyl Amino Purine (BAP) 9 mg L-1, Thidiazuron (TDZ) 1 mg L-1, Zeatin 0,1 mg L-1, dan NAA (0,01 mg L-1, 1 mg L-1). Hasil percobaan menunjukkan bahwa kombinasi BAP 9 mg L-1 dan NAA 0,01 mg L-1 menghasilkan induksi tunas tertinggi pada 12 MST. Pemberian zeatin 0,1 mg L-1 dan NAA 1 mg L-1 menghasilkan panjang tunas tertinggi pada 12 MST.

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Inisiasi Tunas Kokoleceran (Vatica bantamensis) pada Berbagai Jenis Media Tanam dan Konsentrasi BAP (Benzyl Amino Purine) Secara In Vitro

Jurnal Agro ◽

10.15575/1069 ◽

2017 ◽

Vol 4 (1) ◽

pp. 1-14

Author(s):

Sri Sudiyanti ◽

Tubagus Bahtiar Rusbana ◽

Susiyanti Susiyanti

Keyword(s):

Root Number ◽

Mass Propagation ◽

Endemic Plant ◽

Randomized Design ◽

Shoot Number ◽

Two Factors ◽

Completely Randomized Design ◽

Four Levels ◽

Benzyl Amino Purine

Kokoleceran (Vatica bantamensis) is an endemic plant of Banten which is only in Ujung Kulon, and has been designated as identity of Banten province. Now the existence of Kokoleceran has been endangered. Based on data from the IUCN, since 1998 there has been no research. Kokoleceran initiation needs technology for preventing from the extinction. One of the mass propagation is through the technique culture in vitro. This research aimed to get the precise medium and BAP concentration for Kokoleceran, and knowing the response of Kokoleceran growth in in vitro. This research was done in November 2015 until March 2016 at the Laboratory of Biotechnology, Department of Agroecotechnology, Faculty of Agriculture, University of Sultan Ageng Tirtayasa, Serang, Banten. This research used Completely Randomized Design (CRD) two factors. The first factor was medium that were MS and WPM media. The second factor was BAP concentration which consisted of four levels namely 0 mg L-1, 1 mg L-1, 2 mg L-1, and 3 mg L-1. The results showed that the use of different media and BAP concentrations had no impact on the time appear of shoot, shoot number, and root number. There was effect from both treatments on medium color, and growing of callus on the explants.

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Pengaruh Myoinositol dan Arang Aktif terhadap Pertumbuhan Planlet Anggrek Dendrobium dalam Kultur In Vitro

Jurnal Hortikultura ◽

10.21082/jhort.v22n3.2012.p205-209 ◽

2013 ◽

Vol 22 (3) ◽

pp. 205 ◽

Cited By ~ 1

Author(s):

Dyah Widiastoeti ◽

Anggraeni Santi ◽

Nina Solvia

Keyword(s):

Root Growth ◽

In Vitro Culture ◽

Activated Charcoal ◽

Critical Factor ◽

Media Optimization ◽

Randomized Design ◽

Plantlet Growth ◽

Ornamental Crops ◽

Completely Randomized Design

ABSTRAK. Anggrek Dendrobium merupakan tanaman hias komersial yang sangat penting di Indonesia. Optimasi media dalam kultur in vitro sangat diperlukan untuk meningkatkan dan mempercepat pertumbuhan planlet. Salah satu cara untuk mengoptimalisasi media in vitro yaitu dengan pemberian myoinositol dan arang aktif. Tujuan penelitian ialah mengetahui pengaruh myoinositol dan arang aktif terhadap pertumbuhan planlet Dendrobium. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Hias Pasarminggu, Jakarta mulai Bulan Juni sampai dengan Desember 2010. Metode penelitian menggunakan rancangan acak kelompok dengan delapan perlakuan dan lima ulangan. Perlakuan terdiri atas konsentrasi myoinositol 0, 50, 100, dan 150 mg/l dengan dan tanpa penambahan arang aktif. Hasil penelitian menunjukkan bahwa pemberian myoinositol 50 mg/l tanpa arang aktif dapat meningkatkan tinggi planlet, panjang dan lebar daun, sedangkan myoinositol 100 mg/l dengan penambahan arang aktif 2 g/l meningkatkan pertumbuhan jumlah dan panjang akar terbaik. ABSTRACT. Widiastoety, D, Santi, A, and Solvia, N 2012. Effect of Myoinositol and Activated Charcoal on the Growth of Dendrobium Orchid Plantlets in In Vitro Culture. This study was aims to determine the effect of myoinositol and activated charcoal on the growth of Dendrobium plantlets. Dendrobium is one of the most important commercial orchids in Indonesia. Media optimization is critical factor to improve and to promote plantlet growth. One of the methods to enrich the medium was by the use of myoinositol and activated charcoal. The study was conducted at the In Vitro Culture Laboratory of Indonesian Ornamental Crops Plants Research Institute Pasarminggu, Jakarta from June through December 2010. A completely randomized design with eight treatments and five replications was used in this experiment. The treatments given were myoinositol 0, 50, 100, and 150 mg/l with and without addition of activated charcoal. The results showed that application of myoinositol 50 mg/l without activated charcoal enhance the plantlet height, length, and leave width, while myoinositol 100 mg/l with addition of activated charcoal 2 g/l increased the highest number of the root and the longest root growth.

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Evaluation of In Vitro Culture Medium for Conservation of Chrysanthemum Varieties under Different Storage Environment

Buletin Plasma Nutfah ◽

10.21082/blpn.v25n1.2019.p33-42 ◽

2019 ◽

Vol 25 (1) ◽

pp. 33

Author(s):

Lia Sanjaya ◽

Budi Marwoto ◽

Kurniawan Budiarto

Keyword(s):

Low Temperature ◽

In Vitro Culture ◽

Culture Medium ◽

Storage Conditions ◽

Death Rates ◽

Randomized Design ◽

In Vitro Technique ◽

Conservation Of Genetic Resources ◽

Completely Randomized Design

In vitro technique for conservation of genetic resources of chrysanthemum was developed through the application of selected medium under different storage conditions. The research was conducted from June 2014 to October 2016. The experiment was conducted in a completely randomized design which consisted of three chrysanthemum varieties and seven medium formulations and maintained in two storage conditions, i.e. low-temperature and ambient room conditions. The results showed that plantlet of all varieties conserved under ½ MS + 6% sucrose had higher survivals and slighter death rates after 4, 7, and 9 months. Lowtemperature condition provided more suitable circumstances for the respected medium to preserve the plantlet life during 9 months of in vitro storage. Under these conditions, the plantlet survivals ranged 51.3–66.3%. While among the chrysanthemum varieties, Monalisa had highest plantlet survivals than Merahayani and Town Talk.

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Aktivitas Antifungi Air Perasan Bawang Merah (Allium ascalonicum L.) Terhadap Candida albicans Secara In Vitro

Jurnal Ilmu Kedokteran ◽

10.26891/jik.v9i2.2015.71-77 ◽

2017 ◽

Vol 9 (2) ◽

pp. 71

Author(s):

Nurhasanah Nurhasanah ◽

Fauzia Andrini ◽

Yulis Hamidy

Keyword(s):

Candida Albicans ◽

Antifungal Activity ◽

Allium Sativum ◽

Fungicidal Activity ◽

Positive Control ◽

Negative Control ◽

Randomized Design ◽

Significant Difference ◽

Completely Randomized Design

Shallot (Allium ascalonicum L.) has been known as traditional medicine. Shallot which has same genus with garlic(Allium sativum L.) contains allicin that is also found in garlic and has been suspected has fungicidal activity toCandida albicans. It is supported by several researches. Therefore, shallot is suspected has antifungal activity too.The aim of this research was to know antifungal activity of shallot’s water extortion againsts Candida albicans invitro. This was a laboratory experimental research which used completely randomized design, with diffusion method.Shallot’s water extortion was devided into three concentrations, there were 50%, 100% and 200%. Ketoconazole 2%was positive control and aquadest was negative control. The result of this research based on analysis of varians(Anova), there was significant difference between several treatments and was confirmed with Duncan New MultipleRange Test (DNMRT) p<0,05, there was significant difference between 100% shallot’s water extortion with othertreatments, but there was no significant difference between 50% shallot’s water extortion with 200% shallot’s. Theconclusion was shallot’s water extortion had antifungal activity againsts Candida albicans with the best concentration100%, but it was lower than ketoconazole 2%.

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