scholarly journals Characterization of the growth and laccase activity of strains of Pleurotus ostreatus in submerged fermentation

BioResources ◽  
2010 ◽  
Vol 6 (1) ◽  
pp. 282-290
Author(s):  
Rubén Díaz ◽  
Susana Alonso ◽  
Carmen Sánchez ◽  
Araceli Tomasini ◽  
Martha Bibbins-Martínez ◽  
...  
Keyword(s):  
Pleurotus Ostreatus ◽  
Submerged Fermentation ◽  
Culture Medium ◽  
Laccase Activity ◽  
Specific Growth ◽  
Laccase Production ◽  
Specific Rate ◽  
Commercial Exploitation ◽  
Isoenzyme Patterns

Kinetic parameters of growth and laccase activity of five ATCC strains of Pleurotus ostreatus in submerged fermentation were evaluated. The best strain for laccase production and the time of maximum laccase activity were also determined. The greatest laccase activity (37490 U/L), laccase productivity (78 U/L h), specific growth rate (0.026/h), and specific rate of laccase production (119 U/gX h) were observed with the strain of P. ostreatus ATCC 32783. In general, the isoenzyme patterns were different in all the cases; however, all the strains showed two laccase bands in the same position in the gel. Not all strains responded in the same way to the addition of Cu in the culture medium. In general, the sensitivity to Cu could be used to select strains having high laccase activity for commercial exploitation.

scholarly journals Comparative study on laccase activity of white rot fungi under submerged fermentation with different lignocellulosic wastes

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 9166-9179
Author(s):  
Qi An ◽  
Jie Qiao ◽  
Lu-Sen Bian ◽  
Mei-Ling Han ◽  
Xun-You Yan ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Laccase Activity ◽  
White Rot Fungi ◽  
Industrial Applications ◽  
Culture Conditions ◽  
White Rot ◽  
Laccase Production ◽  
Poplar Wood ◽  
Lignocellulosic Wastes ◽  
Cottonseed Hull

Different Pleurotus ostreatus and Flammulina velutipes species were compared relative to their ability to produce laccase in submerged fermentation of various lignocellulosic wastes. Fungi cultivation in identical culture conditions revealed wide differences among both species and strains of the same species. The laccase secretion ability of P. ostreatus strains was superior to F. velutipes strains. Maximum laccase production on cottonseed hull, corncob, and poplar wood was secreted by P. ostreatus CY 568, P. ostreatus CCEF 89, and P. ostreatus CY 568, respectively. The nature of lignocellulosic materials played an important role in determining the expression of laccase potential of fungi. The presence of cottonseed hull improved laccase activity and accelerated the rate of enzyme production. Maximum laccase production on cottonseed hull was nearly 1.29-fold and 1.53-fold higher than that on corncob and poplar wood, respectively. Laccase activity was detected in almost all tested strains on cottonseed hull on the first day, while only a few strains on poplar wood and corncob were detected on the first day. These findings will be helpful for selecting the appropriate strain in industrial applications and for optimization of integrated industrial laccase production.

scholarly journals Optimizing Laccase Production in Auricularia Cornea by Submerged Fermentation With Wheat Bran Extract: Applications in Decolorization of Malachite Green Dye

2021 ◽  
Author(s):  
Li Meng ◽  
Xiaoran Bai ◽  
Qingji Wang ◽  
Xiaobo Li ◽  
Shaoyan Zhang ◽  
...  
Keyword(s):  
Malachite Green ◽  
Wheat Bran ◽  
Submerged Fermentation ◽  
High Performance ◽  
Laccase Activity ◽  
Degradation Products ◽  
Laccase Production ◽  
Malachite Green Dye ◽  
Chromosome Bridge ◽  
Wheat Bran Extract

Abstract Wheat bran extract may enhance laccase activity of Auricularia cornea in submerged fermentation. The laccase activity of A. cornea was maximal (768.78 U/mL) at 26.9 ℃, pH 5, a time point of 6 d, 22 h, and an inducer concentration of 10%. Laccase from A. cornea AC5 decolorized 83.27% of 75 mg/L malachite green within 12 h. High performance liquid chromatography (HPLC) analysis of the extracted metabolites suggested that the decolorization occurred through biodegradation. Malachite green induced cytotoxic damage including formation of a micronucleus and chromosome bridge at anaphase. Degradation products of malachite green proved to be less toxic and had negligible effects on chromosomal aberrations.

scholarly journals The influence of Tween 80 on laccase production by Pleurotus sajor-caju and the efficiency of crude enzyme broth in the removal of bisphenol-A

2018 ◽  
Vol 85 (0) ◽  
Author(s):  
Thaís Silva Teodoro ◽  
Fernanda de Oliveira ◽  
Caroline Poffo ◽  
Laís Pacenko Braga ◽  
Auriciane Arbigaus ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Culture Medium ◽  
Laccase Activity ◽  
C:N Ratio ◽  
Tween 80 ◽  
Crude Enzyme ◽  
Production Costs ◽  
Oxidative Enzymes ◽  
Laccase Production ◽  
Pleurotus Sajor Caju

ABSTRACT: Bisphenol-A is currently considered an environmental pollutant, capable of interfering in the endocrine system of organisms and causing alterations in its development and reproductive system. An alternative method to the chemical treatment of this pollutant has been the use of oxidative enzymes, especially laccases produced by fungi. In order to reduce production costs, agro-industrial waste can be used in the culture medium composition. Nonionic surfactants, which are only slightly toxic to biological membranes, can be applied, as well as Tween 80, to facilitate the excretion of these enzymes into the culture medium. The objectives of this work were: a) characterize the immersion water of banana straw used in the formulation of the culture medium; b) evaluate laccase production by Pleurotus sajor-caju in culture medium with and without addition of Tween 80, through shaken flasks; c) evaluate the efficiency of the crude enzyme broth in degrading bisphenol-A. The shaken flasks were incubated at 30°C for 12 days. The immersion water had a C:N ratio of 13.8, ash percentage of 28.6%, and pH close to neutrality. The addition of Tween 80 on the culture medium (7.5%, m/v) yielded laccase activity and productivity values equal to 3,016.47 U L-1 and 502.7 U L-1 day-1, respectively. These values were 50 and 33.5 times higher than those obtained in the culture medium without addition of Tween 80 for laccase activity and productivity, respectively. The crude enzyme broth degraded 100% of bisphenol-A after 48 hours, regardless of concentration (500, 750 and 1,000 mg L-1).

scholarly journals Copper Induction of Laccase Isoenzymes in the Ligninolytic Fungus Pleurotus ostreatus

2000 ◽  
Vol 66 (3) ◽  
pp. 920-924 ◽  
Author(s):  
Gianna Palmieri ◽  
Paola Giardina ◽  
Carmen Bianco ◽  
Bianca Fontanella ◽  
Giovanni Sannia
Keyword(s):  
Pleurotus Ostreatus ◽  
Culture Medium ◽  
Laccase Activity ◽  
Physiological Activity ◽  
Phenol Oxidase ◽  
Growth Conditions ◽  
White Rot ◽  
Extracellular Laccase ◽  
Cellular Extract ◽  
High Level

ABSTRACT Pleurotus ostreatus is a white rot basidiomycete that produces several extracellular laccase isoenzymes, including phenol oxidase A1b (POXA1b), POXA2, and POXC. POXC was the most abundant isoenzyme produced under all of the growth conditions examined in this study. Copper was the most efficient inducer of laccase activity among the putative inducers tested. The amounts of all of the previously described laccase isoenzymes increased substantially in copper-supplemented cultures. Under these conditions expression of POX isoenzymes was regulated at the level of gene transcription. It is worth noting that poxa1b mRNA was the most abundant induced transcript at all of the growth times analyzed, and the amount of this mRNA increased until day 7. The discrepancy between thepoxa1b transcript and protein amounts can be explained by the presence of a high level of the protein in P. ostreatuscellular extract, which indicated that the POXA1b isoenzyme could be inefficiently secreted and/or that its physiological activity could occur inside the cell or on the cell wall. Moreover, the POXA1b isoenzyme behaved uniquely, as its activity was maximal on the second day of growth and then decreased. An analysis performed with protease inhibitors revealed that the loss of extracellular POXA1b activity could have been due to the presence of specific proteases secreted into the copper-containing culture medium that affected the extracellular POXA1b isoenzyme.

scholarly journals A Preliminary Study on the Newly Isolated High Laccase-producing Fungi: Screening, Strain Characteristics and Induction of Laccase Production

2018 ◽  
Vol 13 (1) ◽  
pp. 463-469
Author(s):  
Qian Wang ◽  
Yingying Qian ◽  
Yuhan Ma ◽  
Changwei Zhu
Keyword(s):  
Aromatic Compounds ◽  
Culture Medium ◽  
Laccase Activity ◽  
Laccase Production ◽  
Control Group ◽  
Dependent Manner ◽  
Plant Materials ◽  
Its Sequence Analysis ◽  
Preliminary Study ◽  
Dose Dependent

AbstractA simple separation method was used in this study to directly separate laccase-producing fungi from withered plant materials. A laccase-producing filamentous fungus was isolated and purified. The strain was highly similar to the species in genus Trametes by ITS sequence analysis, and therefore named Trametes sp. MA-X01. The addition of cupric ions and aromatic compounds to the liquid medium could induce the laccase synthesis in Trametes sp. MA-X01. Copper-induced laccase activity increased in a dose-dependent manner. The highest laccase activity (2138.9 ± 340.2 U/L) was obtained by adding 2.5 mM Cu2+ to the culture medium, which was about 7 times higher than that of the control group. The induction degree of aromatic compounds was different. For the present study, the highest laccase activities were obtained by adding vanillic acid (1007.9±59.5 U/L) or vanillin (981.6±77.2 U/L) to the medium, which were 3.5 and 3.4 times higher than the laccase activity of the control group, respectively.

scholarly journals Determination of optimal conditions for laccase production by Pleurotus ostreatus using sawdust as solid medium and its use in phenol degradation

2012 ◽  
Vol 9 (3) ◽  
pp. 491-499
Author(s):  
Baghdad Science Journal
Keyword(s):  
Pleurotus Ostreatus ◽  
Laccase Activity ◽  
Specific Activity ◽  
Phenol Degradation ◽  
Laccase Production ◽  
Optimum Conditions ◽  
Fungal Isolates ◽  
Extracellular Laccase ◽  
Mycelial Plug

The ability of four local fungal isolates for extracellular laccase production has been tested with five grams 1:1(w/v) humidified sawdust as substrate in mineral salt medium. After 21 day of incubation at 25±1 ? C and using one mycelial plug (5mm), higher level of laccase activity (0.15U/ml) and specific activity (15U/mg) were observed by Pleurotus ostreatus in comparison with other fungal isolates. The results of optimum conditions for laccase production from selected isolate showed that, the maximum laccase activity (0.55U/ml) and specific activity (55U/mg) were obtained at moisture ratio 1:3 (w/v), using 3 mycelial plugs (5 mm), after 15 days incubation period at 25±1 ? C. The results of phenol degradation by crud laccase revealed that, 100% degradation of phenol occurred after 24 hr of incubation at 25 ? C using shaking water bath except at 200mg/l, the remaining phenol was 10.13%.

Incorporation of micro/nanoparticles of PCL with essential oil of Cymbopogon nardus in bacterial cellulose

2018 ◽  
Vol 1 (2) ◽  
pp. 37
Author(s):  
Aline Krindges ◽  
Vanusca Dalosto Jahno ◽  
Fernando Morisso
Keyword(s):  
Particle Size ◽  
Particulate Matter ◽  
Essential Oil ◽  
Zeta Potential ◽  
Bacterial Cellulose ◽  
Culture Medium ◽  
Static Culture ◽  
Cymbopogon Nardus ◽  
Cellulose Membranes

Incorporation studies of particles in different substrates with herbal assets growing. The objective of this work was the preparation and characterization of micro/nanoparticles containing cymbopogon nardus essential oil; and the incorporation of them on bacterial cellulose. For the development of the membranes was used the static culture medium and for the preparation of micro/nanoparticles was used the nanoprecipitation methodology. The incorporation of micro/nanoparticles was performed on samples of bacterial cellulose in wet and dry form. For the characterization of micro/nanoparticles were carried out analysis of SEM, zeta potential and particle size. For the verification of the incorporation of particulate matter in cellulose, analyses were conducted of SEM and FTIR. The results showed that it is possible the production and incorporation of micro/nanoparticles containing essential oil in bacterial cellulose membranes in wet form with ethanol.

scholarly journals A Setup for Microscopic Studies of Ultrasounds Effects on Microliters Scale Samples: Analytical, Numerical and Experimental Characterization

Pharmaceutics ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 847
Author(s):  
Florian N. Gailliègue ◽  
Mindaugas Tamošiūnas ◽  
Franck M. André ◽  
Lluis M. Mir
Keyword(s):  
Culture Medium ◽  
Small Volume ◽  
Acoustic Pressure ◽  
Experimental Characterization ◽  
Focal Distance ◽  
Microscopic Studies ◽  
Cell Membrane Permeabilization ◽  
Ultrasonic Parameters ◽  
Rigid Walls

Sonoporation is the process of cell membrane permeabilization, due to exposure to ultrasounds. There is a lack of consensus concerning the mechanisms of sonoporation: Understanding the mechanisms of sonoporation refines the choice of the ultrasonic parameters to be applied on the cells. Cells’ classical exposure systems to ultrasounds have several drawbacks, like the immersion of the cells in large volumes of liquid, the nonhomogeneous acoustic pressure in the large sample, and thus, the necessity for magnetic stirring to somehow homogenize the exposure of the cells. This article reports the development and characterization of a novel system allowing the exposure to ultrasounds of very small volumes and their observation under the microscope. The observation under a microscope imposes the exposure of cells and Giant Unilamellar Vesicles under an oblique incidence, as well as the very unusual presence of rigid walls limiting the sonicated volume. The advantages of this new setup are not only the use of a very small volume of cells culture medium/microbubbles (MB), but the presence of flat walls near the sonicated region that results in a more homogeneous ultrasonic pressure field, and thus, the control of the focal distance and the real exposure time. The setup presented here comprises the ability to survey the geometrical and dynamical aspects of the exposure of cells and MB to ultrasounds, if an ultrafast camera is used. Indeed, the setup thus fulfills all the requirements to apply ultrasounds conveniently, for accurate mechanistic experiments under an inverted fluorescence microscope, and it could have interesting applications in photoacoustic research.

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