scholarly journals ВПЛИВ МІКРОЕЛЕМЕНТІВ НА НАКОПИЧЕННЯ КАРОТИНОЇДІВ ДЕЯКИМИ ШТАМАМИ БАЗИДІОМІЦЕТІВ

2016 ◽  
Vol 6 (2) ◽  
pp. 181-187
Author(s):  
A. K. Velygodska ◽  
O. V. Fedotov
Keyword(s):  
Culture Filtrate ◽  
Spectrophotometric Method ◽  
Nutrient Medium ◽  
Gravimetric Method ◽  
Growth Processes ◽  
Fomes Fomentarius ◽  
Experimental Environment ◽  
Dry Biomass ◽  
Peptone Medium ◽  
Acetone Extracts

<p>The effect of microelements on growth and accumulation of carotenoids highly productive strains of basidiomycetes at surface cultivation on glucose-peptone medium was investigated. The objects of research are 3 wood destroying strain. There are <em>Laetiporus sulphureus</em> (Bull.) Murrill Ls-08, <em>Fomes fomentarius</em> (L.) Fr. Ff-1201 from the order <em>Polyporales</em> and <em>Fistulina hepatica</em> (Schaeff.) Sibth Fh-18 from the order <em>Agaricales</em>. Research materials are strains mycelium and culture filtrate (CF). Absolutely dry biomass (ADB) mycelium was determined by the gravimetric method, the content of carotenoids was determined by spectrophotometric method in acetone extracts of the Vetshteyn formula. Established individual influence of microelements on the accumulation of biomass and carotenoids of basidiomycetes strains. The possibility of the regulation of these processes by introducing into the glucose-peptone medium of various Fe, Cu, Zn, Ni and Mn sulphate. So, the best to increase the intensity of the growth processes and the accumulation of carotenoids strain of<em> L. sulphureus</em> Ls-08 is an experimental environment which includes Zn sulfate in a concentration of 8 mmol/L. To induce the accumulation of ADB and carotenoids in the mycelium and CF of strain <em>F fomentarius</em> Ff-1201 making in is expedient Mn sulfate in a concentration of 1.6 mmol/L. To improve carotenogenesis of <em>F. hepatica</em> Fh-18 strain expedient entry in GPM Mn sulphate at concentration of 8 mmol/L. These allow to optimize the concentration of microelements in nutrient medium for the cultivation of carotenoids high-producing strains of Basidiomycetes.</p><p align="left"> </p>

ВПЛИВ ДЖЕРЕЛ АЗОТНОГО ЖИВЛЕННЯ НА СИНТЕЗ КАРОТИНОЇДІВ ДЕЯКИМИ ШТАМАМИ БАЗИДІОМІЦЕТІВ

2014 ◽  
Vol 4 (01) ◽  
pp. 22
Author(s):  
A. K. Veligodska ◽  
O. V. Fedotov ◽  
A. S. Petreeva
Keyword(s):  
Culture Filtrate ◽  
Nutrient Medium ◽  
Culture Fluid ◽  
Carotenoid Content ◽  
Nitrogen Compounds ◽  
Total Carotenoid ◽  
Carotenoid Accumulation ◽  
Total Carotenoid Content ◽  
Peptone Medium ◽  
Acetone Extracts

<p>The influence of certain nitrogen compounds - components of glucose-peptone medium (GPM) on the accumulation of carotenoids by some strains was investigated by surface cultivating basidiomycetes. The total carotenoid content was set in acetone extracts of mycological material spectrophotometrically and calculated using the Vetshteyn formula.</p> <p>As the nitrogen-containing components used GPM with 9 compounds, such as peptone, DL-valine, L-asparagine, DL-serine, DL-tyrosine, L-proline, L-alanine, urea, NaNO<sub>3</sub>. The effect on the accumulation of specific compounds both in the mycelium and in the culture fluid of carotenoids by culturing certain strains of Basidiomycetes was identified.</p> <p>Adding to standard glucose-peptone medium peptone at 5 g/l causes an increase of carotenoid accumulation by strain <em>L. sulphureus</em> Ls-08, and in a concentration of 4 g/l by strains of <em>F. hepatica </em>Fh-18 and <em>F. fomentarius</em> Ff-1201.</p> <p>In order to increase the accumulation of carotenoids in the mycelium  we suggested to make a standard glucose-peptone medium with proline or valine for cultivating of <em>L. sulphureus</em> Ls- 08 strain; alanine for <em>F. fomentarius</em> Ff-1201 strain; proline, asparagine and serine - for strain Fh-18 of <em>F. hepatica</em>. The results can be implemented in further optimization of the composition of the nutrient medium for culturing strains of Basidiomycetes wich producing carotenoids.</p> <p><em>Keywords: nitrogen-containing substances, Basidiomycetes, mycelium</em><em>,</em><em> culture filtrate, carotenoids</em></p>

scholarly journals ВПЛИВ ПЕВНИХ ВІТАМІНІВ НА СИНТЕЗ ПОЛІФЕНОЛЬНИХ РЕЧОВИН ДЕЯКИМИ БАЗИДІОМІЦЕТАМИ

2013 ◽  
Vol 3 (03) ◽  
pp. 24
Author(s):  
А. К. Veligodska ◽  
О. V. Fedotov
Keyword(s):  
Culture Filtrate ◽  
Polyphenolic Compounds ◽  
Fomes Fomentarius ◽  
Laetiporus Sulphureus ◽  
Dry Biomass ◽  
Species Effect

<p>We studied the influence of certain vitamins on the intensity of the synthesis of polyphenolic compounds and carotenoids by some Basidiomycetes strains, such as <em>Laetiporus sulphureus</em> Ls-08, <em>Fomes fomentarius </em>Ff-1201 and <em>Fistulina hepatica</em> Fh-18. The registration of accumulation of dry biomass and content of polyphenols and carotenoids in the mycelia and culture filtrate of strains that were cultivated on glucose-peptone substrates (GPS) with vitamins was performed. The vitamins A, E, C, B<sub>1</sub>, B<sub>12</sub>, and PP at the concentration of 0.005, 0.01 and 0.05 g/l were applied as modification of GPS. We founded the species effect on the synthesis of vitamins, polyphenols, and carotenoids. We suggested separate application of vitamins A, E, B<sub>1</sub>, and B<sub>12</sub> at concentration of 0.01 g/ l to induce the synthesis of polyphenols and carotenoids. Results of the study will be used to develop a modification of GPS for the cultivation of strains of polyphenolic substances of basidiomycete origin.</p> <p><em>Keywords: Basidiomycetes, mycelium, culture filtrate, polyphenols, carotenoids, vitamins.</em></p>

scholarly journals The lipid peroxidation intensity of fungi strains from the orders Agaricales and Polyporales

2016 ◽  
Vol 24 (2) ◽  
Author(s):  
O. V. Fedotov
Keyword(s):  
Lipid Peroxidation ◽  
Culture Filtrate ◽  
Biomass Accumulation ◽  
Thiobarbituric Acid ◽  
Systematic Position ◽  
Metabolic Products ◽  
Dry Biomass ◽  
Weighing Method ◽  
Peptone Medium ◽  
Rates Of Growth

This article is devoted to investigation of the dynamics of growth and level of spontaneous and induced lipid peroxidation intensity of Basidiomycetes strains grown by surface cultivation on a glucose-peptone medium. The materials of the research are mycelium and culture filtrates (CF) of 57 strains (5 belong to 5 species from the order Polyporales s.l., and 52 belong to 7 species of the order Agaricales s.l.). To study the dynamics of growth we used a weighing method for determining the accumulation of absolutely dry biomass. Intensity of lipid peroxidation was determined by a modified spectrophotometric method for content of active to thiobarbituric acid products. It was found that the most productive in absolutely dry biomass accumulation were the strains Flammulina velutipes (Curt.: Fr.) Sing. F-610 and Pleurotus eryngii (DC.: Fr.) Quél. P-er. The level of spontaneous and induced LPO intensity in mycelia of all strains was higher than this figure in the culture filtrate and increased with the duration of cultivation. Dependencies between the content of lipid peroxidation products in the mycelia and CF were not established. The lowest values were recorded for biomass accumulation by the strains Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. P-14, P-192 and P. citrinopileatus Singer. Р-сіtr. Groups of basidiomycete cultures with different levels of TBA-AP were identified. Spontaneous and induced intensivity of lipid peroxidation in all studied strains of mycelia was higher than the figure in the culture filtrate. The intensity of lipid peroxidation in both mycelia and culture filtrate constantly increased, which can be explained by the growing shortage of certain nutrients (primarily carbon) and increased concentration of metabolic products in the medium. The ratio of spontaneous and induced lipid peroxidation intensity is specific to each strain and is independent of its systematic position. Shifting of prooxidant-antioxidant balance to a relatively stationary level is a mark of stress reaction. LPO-products can be both inductors and primary mediators of stress as a special class of biological systems. Selected strains with high rates of growth and LPO ntensity are promising for applications in biotechnology and ecology.

scholarly journals РЕГУЛЯЦІЯ СИНТЕЗУ ПОЛІФЕНОЛЬНИХ РЕЧОВИН ДЕЯКИМИ ШТАМАМИ БАЗИДІОМІЦЕТІВ

2014 ◽  
Vol 4 (01) ◽  
pp. 35
Author(s):  
O. V. Fedotov ◽  
A. K. Veligodska
Keyword(s):  
Carboxylic Acids ◽  
Culture Filtrate ◽  
Spectrophotometric Method ◽  
Total Content ◽  
Peptone Medium

<p>The effect of specific carbon-containing compounds as additional components glucose-peptone medium (GPM), the intensity of the polyphenolic substances and carotenoids synthesis by some strains was investigated by surface cultivating basidiomycetes. The total content of polyphenolic substances set out in alcoholic extracts of the modified procedure by Folin-Chokalteu and in acetone carotenoids extracts of mycological material by spectrophotometric method and calculated by Vetshteyn formula. In GPM we used 13 carbonaceous components compounds belonging to mono-, oligo- and polysaccharides and carboxylic acids The effect of the 13 carbon-containing compounds on the accumulation of biomass, carotenoids and polyphenols Basidiomycetes strains <em>L. sulphureus</em> Ls-08, <em>F. fomentarius</em> Ff-1201 and <em>F. hepatica</em> Fh-18 was identified. For the purpose of inducing the synthesis of carotenoids by strains Ls-08 and Fh-18 may recommend changes in the standard GPS by fructose, and for strain Ff-1201 by sucrose. In order to induce synthesis of polyphenols strains Ff-1201 and Fh-18 to make appropriate standard GPS by mannose and for strain Ls-08 by sucrose.</p> <p><em>Keywords: Basidiomycetes, mycelium, culture filtrate, polyphenols, carotenoids</em></p>

scholarly journals Influence of siderophores and iron on Mycobacterium bovis isolation from pathological material

2021 ◽  
Vol 7 (1-2) ◽  
pp. 21-26
Author(s):  
A. I. Zavgorodniy ◽  
S. A. Pozmogova ◽  
V. V. Bilushko ◽  
Kalashnyk Kalashnyk ◽  
O. I. Gologurska
Keyword(s):  
Mycobacterium Bovis ◽  
Culture Filtrate ◽  
Nutrient Medium ◽  
Culture Media ◽  
Alcoholic Extract ◽  
Simultaneous Presence ◽  
Growth Properties ◽  
Pathological Material ◽  
Bacterial Mass

The article presents the results of studying the effect of siderophores and iron on the isolation of Mycobacterium bovis from pathological material. It has been established that the simultaneous presence of iron and siderophore from M. phlei in the nutrient medium makes it possible to detect the growth of M. bovis from pathological material 6–8 days earlier; ensures the growth of more colonies and bacterial mass. The presence of heterologous to mycobacteria siderophore (from Nocardia spp.) in the medium reduces the elective (growth) properties of the medium. Siderophores found in the culture filtrate or alcoholic extract of M. phlei can be valuable additives to culture media for the accelerated isolation of M. bovis from pathological material

AMINO ACIDS AND PROTEINS IN CULTURE FILTRATES OF THE ANTHRACNOSE PATHOGEN FUNGUS COLLETOTRICHUM LINI

2020 ◽  
pp. 121-127
Author(s):  
N. V. Proletova ◽  
Keyword(s):  
Amino Acids ◽  
Culture Filtrate ◽  
Nutrient Medium ◽  
Culture Medium ◽  
Protein Composition ◽  
Entire Period ◽  
Culture Filtrates ◽  
Russian Research Institute ◽  
Russian Research

The research was carried out on the basis of laboratory biotechnologies of All-Russian research institute of flax (Tver region) in 2010–2012, 2016. The aim of the work was to determine the amino acid and protein composition of culture filtrates of the anthracnose pathogen fungus Colletotrichum lini Manns et Bolley in order to adjust the concentration of selective agent in the nutrient medium when creating in vitro new flax genotypes resistant to anthracnose. It was established that the culture filtrates of strains 527 and 608 contain such amino acids as alanine, glycine, asparagine, cysteine, threonine, aspartic acid, glutamic acid, as well as arginine in strain 527 and traces of tyrosine and lysine in strain 608. It was established that the concentration of amino acids in EC of strain 527 was significantly higher than in culture filtrate of strain 608. It was shown that the toxicity of the culture filtrate depended on the degree of aggressiveness of the anthracnose pathogen strain – culture filtrate of a strongly aggressive strain is more toxic than the culture filtrate of a weakly aggressive strain. Studies have revealed that when cultivating the fungus-causative agent of anthracnose on a nutrient medium, as the mycelium of fungus grew, the concentrations of asparagine, alanine, aspartic and glutamic acids, and glycine decreased in the culture filtrates. It was established that the change in amount of proteins happened during the entire period of cultivation of the mycelium of fungus on a liquid nutrient medium. It is shown that accumulation and content of proteins in culture filtrates of strains of different aggressiveness occurs in different ways. The more aggressive strain is (639), which is more toxic, contains and accumulates more proteins in the culture medium during the entire period of growth and development the less aggressive strain is (419).

scholarly journals A Simple Spectrophotometric Method for the Estimation of .ALPHA.-Carotene, .BETA.-Carotene and Lycopene Concentrations in Carrot Acetone Extracts

2007 ◽  
Vol 54 (7) ◽  
pp. 351-355 ◽  
Author(s):  
Masayasu Nagata ◽  
Yuji Noguchi ◽  
Hidekazu Ito ◽  
Shunsuke Imanishi ◽  
Keita Sugiyama
Keyword(s):  
Spectrophotometric Method ◽  
Beta Carotene ◽  
Acetone Extracts ◽  
Carotene Beta

scholarly journals ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПЕРЕКИСНОГО ОКИСНЕННЯ ЛІПІДІВ КСИЛОТРОФНИХ БАЗИДІОМІЦЕТІВ У ГЛИБИННІЙ КУЛЬТУРІ

2013 ◽  
Vol 3 (02) ◽  
pp. 220
Author(s):  
A. V. Chaika ◽  
O. V. Fedotov
Keyword(s):  
Lipid Peroxidation ◽  
Growth Rate ◽  
Culture Filtrate ◽  
Submerged Fermentation ◽  
Gravimetric Method ◽  
Correlation Coefficients ◽  
High Growth ◽  
Its Region ◽  
Thiobarbituric Acid ◽  
Xylotrophic Basidiomycetes

<p>The aim of the study was to determine the lipid peroxidation (LP) intensity of xylotrophic basidiomycetes cultures under submerged fermentation on glucose-peptone medium. The materials were submerged mycelium and culture filtrate of 79 strains of 18 species xylotrophic basidiomycetes. Among the studied strains, 60 strains belong to the order <em>Agaricales</em> and 19 – to order <em>Polyporales</em>. Most of the strains (85%) were isolated from the fruiting bodies collected in different localities of Donetsk city and its region. The following methods were used. Oven-dry biomass was determined by gravimetric method and biomass increase and specific growth rate were calculated. The pH of the culture filtrate was determined by potentiometric method. The intensity of lipid peroxidation processes was estimated with the modified thiobarbituric acid test. The study concluded that among the studied strains high level of LP in the mycelium is typical for the <em>F. velutipes</em> cultures, particularly the F-vv, F-03 and F-1. The low intensity of LP in the mycelium was established for the <em>F. fomentarius</em> Ff-09, Ff-1201 strains and the <em>L. edodes</em> Le-2, Le-4, Le-6, Le-7 strains. The high content of LP products in the culture filtrate is typical in most <em>Polyporales</em> cultures, for example, in the strains of <em>I. lacteus</em> IL-1201, <em>T. hirsuta</em> Th-11, <em>D. quercina</em> Dq-08 and <em>F. fomentarius</em> T-10. Paucity of the LP products in the culture filtrate established for some strains of <em>F. velutipes</em>, for example, F-204, F-11, <em>S. commune</em> Sc-1102 strain and <em>G. lucidum</em> cultures. Direct dependence between the content of LP products in the mycelium and culture filtrate was not established. Calculated correlation coefficients for the investigated strains showed the dependence of the growth rates and the intensity of LP in the mycelium and culture filtrate. Selected strains with high growth rate and a significant LP level in the culture filtrate are promising in technologies of lignocellulosic wastes and xenobiotics biodegradation and environment bioremediation.</p> <p><em>Key words: xylotrophic basidiomycetes, submerged fermentation, lipid peroxidation</em></p>

Influence of polyphenols on the formation of resistance of callus cultures of sunflower plants to the exometabolites of the fungus Sclerotinia sclerotiorum

2021 ◽  
Vol 1 (2) ◽  
pp. 75-82
Author(s):  
E. A. Kalashnikova ◽  
◽  
S. M. Zaytseva ◽  
T. T. Nguen ◽  
R. M. Kirakosyan ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Secondary Metabolites ◽  
Sclerotinia Sclerotiorum ◽  
Culture Filtrate ◽  
Nutrient Medium ◽  
Callus Cultures ◽  
Stress Factor ◽  
Biosynthetic Activity ◽  
Mushroom Culture

When studying the formation of secondary metabolites represented by phenolic compounds in callus cultures of sunflower plants, it was found that in vitro under the influence of a stress factor, the accumulation of polyphenols increases. It was found that the biosynthetic activity of callus cultures in relation to polyphenols increases in proportion to the concentration of the mushroom culture filtrate Sclerotinia sclerotiorum in the nutrient medium.

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