scholarly journals A Simple Spectrophotometric Method for the Estimation of .ALPHA.-Carotene, .BETA.-Carotene and Lycopene Concentrations in Carrot Acetone Extracts

2007 ◽  
Vol 54 (7) ◽  
pp. 351-355 ◽  
Author(s):  
Masayasu Nagata ◽  
Yuji Noguchi ◽  
Hidekazu Ito ◽  
Shunsuke Imanishi ◽  
Keita Sugiyama
Keyword(s):  
Spectrophotometric Method ◽  
Beta Carotene ◽  
Acetone Extracts ◽  
Carotene Beta

scholarly journals Clinical Evaluation of Three Carotenoid Containing Supplements Using Fingertip Reflection Spectroscopy Measurements (P02-009-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Dawna Salter-Venzon ◽  
Kevin Gellenbeck ◽  
Chun Hu
Keyword(s):  
Vegetable Intake ◽  
Beta Carotene ◽  
Reflection Spectroscopy ◽  
Open Label ◽  
Open Label Study ◽  
Funding Sources ◽  
Supplement Group ◽  
Supplement Usage ◽  
History Of ◽  
Carotene Beta

Abstract Objectives Recent technology improvements have allowed measurement of carotenoid pigments utilizing simple reflection spectroscopy fingertip measurements (Veggie Meter®) (1). We used this technique to measure the effect of ingestion of three different dietary supplements containing carotenoids of different types and potencies: Vision Health with Lutein, Multi Carotene (beta carotene, alpha carotene, lutein, zeaxanthin), or Concentrated Fruits & Vegetables (lutein, lycopene) (2). Methods In an open label study, a total of 24 volunteers (8 for each of the study products) consumed the supplements daily according to label recommendations. Veggie Meter® measurements were taken on Day 0 and Weeks 2, 4, 6, 8, 10 and 12. Supplements were consumed for the first 6 weeks and then were discontinued for the following 6 weeks. Questionnaires were used at baseline, 6 and 12 weeks to obtain a brief history of supplement usage and fruit/vegetable intake. Results In most subjects, the veggie meter measurement increased from baseline after 6 weeks of supplementation, which then returned towards the baseline in the six weeks after supplementation was stopped. Responses from the three supplements varied in magnitude based on dosage and carotenoid type. There was also noticeable variation in individual responses within each supplement group. Conclusions Reflection spectroscopy fingertip measurements provided data supporting the notion that carotenoids deposit in the skin in response to supplementation with carotenoid containing supplements. This may suggest this method is a useful to monitor carotenoid status and indicate when diet modifications would be beneficial to individuals. Funding Sources Funding for the study was provided by Access Business Group/Amway.

scholarly journals AKTIVITAS HEPATOPROTEKTOR SARI BUAH LABU KUNING (Cucurbita moschata Duchesne.) PADA TIKUS (Rattus norvergicus) YANG DIINDUKSIKAN KARBON TETRAKLORIDA

2016 ◽  
Vol 2 (1) ◽  
pp. 18-23
Author(s):  
Indrawan Aditya ◽  
Ihwan Ihwan ◽  
Jamaluddin Jamaluddin
Keyword(s):  
Experimental Research ◽  
Beta Carotene ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Cucurbita Moschata ◽  
The Third ◽  
Body Tissues ◽  
Risk Of Cancer ◽  
Carotene Beta

One of the plants that are often used in traditional medicine is the fruit of pumpkin used to treat hepatitis manner pumpkin fruit cut into small pieces and then steamed, then eaten .Pumpkin fruits contain active compounds such as saponins, tannins, flavonoids and beta-carotene. Beta-carotene is converted in the body into vitamin A is beneficial for the growth, maintenance of body tissues and to reduce the risk of cancer and heart disease. This study aims to determine pumpkin juice has a hepatoprotective activities on liver function by looking at parameters SGPT and SGOT in rats after induced CCl4. Type of experimental research using True Experimental research design design. Test animals were grouped into 5 groups, each group consisting of 3 rats. The first group of positive control (Methicol®), the second group negative control (CCl4), the third group of pumpkin juice 25%, the fourth group of pumpkin juice 50%, the fifth group of pumpkin juice 75%. From the results of this study concluded that the pumpkin juice 75% effective as a hepatoprotective by looking at parameters SGPT is 48 U/liter and SGOT is 66 U/liter in rats after induced carbon tetrachloride

Carotenoids from Malanga (Xanthosoma Sagittifolium) Leaves Protect Cells Against Oxidative Stress in Rats

2005 ◽  
Vol 75 (2) ◽  
pp. 161-168 ◽  
Author(s):  
Arruda ◽  
Souza ◽  
Siqueira
Keyword(s):  
Vitamin A ◽  
Protein Oxidation ◽  
Beta Carotene ◽  
Thiobarbituric Acid ◽  
Protein Carbonyl ◽  
Rat Tissues ◽  
Liver Protein ◽  
Xanthosoma Sagittifolium ◽  
Leaf Powder ◽  
Carotene Beta

The present study investigated the effect of malanga leaf powder and its carotenoids oil extract on oxidative damage in rat tissues. A group of rats received AIN-93G diet devoid of vitamin A (AD) and three other groups were treated with AD diet supplied with synthetic beta-carotene (beta-car) or malanga leaf powder (MP) or malanga carotenoids extract (CE). The diets’ carotenoids bioavailability was determined through carotenoids intake/liver retinol content. Lipid thiobarbituric acid reactive substances (TBARS) and protein (carbonyl) oxidation, and reduced (GSH) and oxidized (GSSG) glutathione concentrations were determined in liver, heart, and brain. Synthetic beta-carotene bioavailability was higher than that of pro-vitamin A carotenoids from MP and CE diets, and no difference was observed between the latter two groups. Liver and heart lipid peroxidation was lower in the beta-car and CE groups than the AD group, while no difference was observed for the MP group. The beta-car, MP, and CE groups showed lower liver protein oxidation than the AD group, and only the CE group had lower heart protein oxidation in relation to AD. The MP group had a lower liver GSSG concentration and higher GSH/GSSG ratio than the AD group, while no difference was observed for heart glutathione concentration among the groups. The results indicate that at physiological levels, beta-car, malanga carotenoids extract, and malanga leaf powder have antioxidant effects in rats.

Vitamin and Nutritional Status in Cuban Smokers and Nonsmokers in the Context of an Emerging Epidemic Neuropathy

2000 ◽  
Vol 70 (3) ◽  
pp. 126-138 ◽  
Author(s):  
Jacques Barnouin ◽  
Rafael Pérez Cristiá ◽  
Michelle Chassagne ◽  
Tomás Barrios Verdura ◽  
Josiane Arnaud ◽  
...  
Keyword(s):  
Beta Carotene ◽  
Exposure Level ◽  
Dietary Intakes ◽  
Total Carotenoids ◽  
Blood Concentrations ◽  
Protein Levels ◽  
Cyanide Detoxification ◽  
Underlying Mechanisms ◽  
Copper Delivery ◽  
Carotene Beta

A prospective survey was conducted in a random sample of smokers (SMO) and non-smokers (NON) men living in Havana. The aim of the survey was to investigate metabolic and dietary parameters discriminating between SMO and NON volunteers, which could explain underlying mechanisms to the higher risk of Epidemic Neuropathy (51000 reported cases in Cuba from late 1991) previously demonstrated in the Cuban smoker. Forty three blood markers were determined and dietary intakes evaluated during 4 seasonal periods. alpha-carotene, beta-carotene, beta-cryptoxanthin, total carotenoids and riboflavin blood concentrations were lower in SMO vs. NON subjects. Activities of glutathione reductase and butryrylcholinesterase were lower in the SMO group, as did total protein levels. Copper and thiocyanates were higher in SMO subjects. Most of the dietary parameters did not differ between the two groups of volunteers. Low biologically available quantities of carotenoids and riboflavin from low dietary intakes and depletion through smoking would be the main precipitating factors of Epidemic Neuropathy. Protein depletion and copper delivery via tobacco smoke, sunlight exposure, level of physical activity and high consumption of cigarettes, in a context of low dietary supplies concerning especially B vitamins, were probably aggravating factors through oxidative stress and impairment of cyanide detoxification.

Effects of Epoxycarotenoids, beta-Carotene, and Retinoic Acid on the Differentiation and Viability of the Leukemia Cell Line NB4 in Vitro

1999 ◽  
Vol 69 (5) ◽  
pp. 303-308 ◽  
Author(s):  
Duitsman ◽  
Barua ◽  
Becker ◽  
Olson
Keyword(s):  
Cell Line ◽  
Cell Mass ◽  
Leukemia Cell ◽  
Beta Carotene ◽  
Viable Cell ◽  
Leukemia Cell Line ◽  
P Values ◽  
A Cell ◽  
Carotene Beta

Three all-trans epoxides of beta-carotene (beta-Car), namely, 5,6-epoxy-beta-carotene (5,6-EC), 5,8-epoxy-beta-carotene (5,8-EC) and 5,6,5’,6’-diepoxy-beta-carotene (5,6,5’,6’-DEC) were synthesized by treatment of beta-carotene with 3-chloroperoxybenzoic acid, were purified chromatographically, and were characterized. The relative potencies (mean ± S.D.) of 1 mM compounds in inducing the differentiation of NB4 cells, a cell line that contains the chromosomal transposition t(15;17) characteristic of acute promyelocytic leukemia, after 4 days of incubation were: RA: 1.35 ± 0.16, 5,6-EC: 0.29 ± 0.01, 5,8-EC: 0.22 ± 0.05, 5,6,5’,6’-DEC: 0.11 ± 0.02, betaC: 0.09 ± 0.01, and the control: 0.06 ± 0.01. The same order of potencies existed at other concentrations tested and at other incubation times. P values for the differences between the inducing activities of successive pairs of compounds at 1 muM were: RA vs. 5,6-EC, < 0.001; 5,6-EC vs. 5,8-EC, < 0.01; 5,8-EC vs. 5,6,5’,6’-DEC, < 0.01; 5,6,5’,6’-DEC vs. beta-Car, < 0.10; beta-Car vs. control, < 0.005. Similar P values were also obtained for studies at other concentrations and at other incubation times. The viable cell mass at 4 days was inversely proportional to the extent of differentiation (rs = –1.0). The inducing activities of all compounds were dose-dependent. Thus, the 5,6-monoepoxide of beta-carotene, which has not previously been studied as an inducer, showed higher activity in NB4 cell differentiation than the 5,8-monoepoxide, the 5,6,5’,6’-diepoxide, or beta-carotene. Possible explanations of these observations are discussed.

Effect of Boiling on Carotenoids content of Pumpkin available in Damascus, Syria

2021 ◽  
pp. 5642-5646
Author(s):  
Hala Juha ◽  
Fida Amali
Keyword(s):  
Linear Regression ◽  
Correlation Coefficient ◽  
Spectrophotometric Method ◽  
Analytical Method ◽  
Beta Carotene ◽  
Local Market ◽  
Beer's Law ◽  
Beer’S Law ◽  
Standard Solution ◽  
Regression Correlation

The carotenoids contents of pumpkin were determined in this study by spectrophotometric method. This method was applied on beta-carotene as standard solution as well as samples. The absorption were determined at λmax 454 nm, the analytical method was validated by evaluating linearity, precision, accuracy, LODs, and LOQs of the analyts. Beer’s law is obeyed over the concentration of (0.3-9) µg/ml with a linear regression correlation coefficient of 0.9986, and recovery in range from 98.5-102.2%. After the validation was performed using standard solution, the method then was applied on samples of pumpkin available in local market in Syria after extraction the carotenoids by using Acetone/ Dichloromethane 70/30 .The results ranged between 216.46 - 80.56 µg/g. During boiling for pumpkin, the level of carotenoid reduced between 0.45-13.26 %, 0.18 – 23.07%, 17.3-40.54%, 22.65-51.73%, 14.91_55.82% and 26.16 – 72.56%, for 5, 10, 15, 30, 45 and 60 min respectively. According to the obtained result results, it is concluded that the boiling reduced carotenoid at different rates.

scholarly journals PERKEMBANGAN PERAKITAN VARIETAS DAN TEKNIK BUDI DAYA JAGUNG ANTIOKSIDAN SEBAGAI PANGAN FUNGSIONAL

2018 ◽  
Vol 37 (1) ◽  
pp. 33
Author(s):  
M Yasin HG ◽  
A. Haris Talanca ◽  
M Jana Mejaya
Keyword(s):  
Hair Loss ◽  
Functional Food ◽  
Raw Materials ◽  
Beta Carotene ◽  
Plant Spacing ◽  
Pollen Type ◽  
Body Tissues ◽  
Carotene Beta ◽  
Early Blindness

<p align="center">ABSTRAK</p><p>Jagung antioksidan mengandung beta karoten pada endosperm yang bermanfaat untuk kesehatan, antara lain dapat mencegah buta dini (rabun/katarak), kerontokan rambut, memperkuat jaringan tubuh, dan mencegah gizi buruk pada anak balita. Kementerian Pertanian telah melepas tiga varietas jagung antioksidan kaya beta karoten, dua dari jenis bersari bebas (varietas Provit A1 dan Provit A2) dan satu dari jenis hibrida (varietas Bima Provit A1) masing-masing dengan kadar beta karoten 155,3-281,6% lebih tinggi dari jagung biasa. Potensi hasil jenis bersari bebas dapat mencapai 7,36 t/ha dan jenis hibrida 9,85 t/ha. Budi daya jagung antioksidan sama dengan jagung biasa. Aspek yang perlu mendapat perhatian adalah isolasi jarak tanaman, minimal 300 m, dan isolasi waktu tanam 3 minggu setelah tanam dengan tanaman jagung biasa. Hal ini diperlukan untuk menghindari xenia effect, yaitu pengaruh tepungsari jagung biasa yang menyerbuki jagung antioksidan sehingga kadar antioksidannya akan menurun dan statusnya kembali sama dengan jagung biasa. Hasil panen dapat dijadikan bahan baku industry berbagai makanan olahan bergizi tinggi. Pengembangan jagung antioksidan, terutama di Kawasan Timur Indonesia perlu peran pemerintah setempat dalam upaya mempercepat adopsi oleh petani.</p><p>Kata kunci: Jagung, antioksidan, beta karoten, pangan fungsional</p><br /><p align="center"><strong> </strong></p><p align="center">ABSTRACT</p><p>Antioxidant maize contains beta carotene in the endosperm that is beneficial for health, such as can prevent early blindness (blindness / cataract), hair loss, strengthen body tissues, and prevent malnutrition in children under five. The Ministry of Agriculture has released three varieties of beta-carotene-rich antioxidant maize, two of the free-range (Provit A1 and Provit A2) varieties and one of the hybrid varieties (Bima Provit A1) each with carotene beta levels of 155.3 to 236.6 % higher than ordinary corn. The potential of free pollen type yield can reach 7.36 t / ha and hybrid type 9.85 t / ha. The cultivation of antioxidant corn is the same as ordinary corn. Aspects that need attention are isolation of plant spacing, at least 300 m, and isolation of planting time 3 weeks after planting with ordinary corn crops. This is necessary to avoid the xenia effect, which is the effect of ordinary corn corn that pollinate antioxidant corn so that its antioxidant levels will decrease and its status is the same as ordinary corn. Yields can be used as industrial raw materials of various highly processed nutritious foods. Development of antioxidant maize, especially in Eastern Indonesia needs the role of local government in an effort to accelerate adoption by farmers.</p><p>Keywords: Maize, antioxidant, beta caroten, functional food.</p>

​Effect of Beta-carotene Supplementation on Plasma Carotene Content and Fertility of Lactating Crossbred Sahiwal Cows

2021 ◽  
Author(s):  
Vinod Bhateshwar ◽  
Dinesh Chandra Rai ◽  
Raj Kumar Duary
Keyword(s):  
Blood Plasma ◽  
Post Partum ◽  
Beta Carotene ◽  
Fertility Rate ◽  
Control Group ◽  
Carotene Content ◽  
Cont Group ◽  
Sahiwal Cows ◽  
Carotene Beta ◽  
Beta Group

Background: Beta-carotene (BETA) is a precursor of retinol (Vitamin A) and positively influenced on reproductive efficiency in cows. The aim of the study was to determine the effect of orally supplementation of beta-carotene on plasma carotene content and fertility rate of lactating crossbred Sahiwal cows. Methods: Twenty-four disease-free lactating crossbred Sahiwal cows with a close date of calving were randomly divided into two homogeneous groups for Control group (CONT; n = 12) and Beta-carotene group: 500 mg/cow/d (BETA; n = 12) from 7 days post partum (dpp) until 105 dpp thereafter on concentrations of beta-carotene and selected protein and energy parameters in plasma were determined. In addition, effects on fertility rate were studied. Result: Beta-carotene concentrations increased in plasma of beta-carotene (BETA-group) supplemented cows compared to control (CONT-group) cows (p less than 0.001). In BETA-group cows, urea concentration in blood plasma decreased significantly compared to cows of CONT-group (p less than 0.001) but did not differ in total protein, albumin, glucose and total cholesterol content in cows of both groups. Occurrence of first post partum estrus decreased (p less than 0.001) in beta-carotene (BETA-group: 74 dpp) cows compared to control (CONT-group: 81 dpp) cows. In this study, it was observed that the overall conception rate was higher (p greater than 0.001) in BETA-group: 83.33% (cows pregnant: 10/12) and lesser in the CONT-group: 50.00% (cows pregnant: 6/12). The results of this study indicated that oral supplementation with beta-carotene increased the concentration of beta-carotene and decreased the concentration of urea in plasma and cows with higher blood plasma beta-carotene content improved fertility over cows with lower blood plasma beta-carotene content.

INTESTINAL ABSORPTION OF D-XYLOSE (dX) AND beta-CAROTENE (beta C) IN CRITICALLY ILL PATIENTS

1998 ◽  
Vol 26 (Supplement) ◽  
pp. 90A
Author(s):  
Alberto Zuniga ◽  
Jorge Pedroza ◽  
Norma Lopez ◽  
Alejandra Marquez ◽  
Guillermo Dominguez-Cherit ◽  
...  
Keyword(s):  
Intestinal Absorption ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Beta Carotene ◽  
Carotene Beta
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