scholarly journals ІНТЕНСИВНІСТЬ ПРОЦЕСІВ ПЕРЕКИСНОГО ОКИСНЕННЯ ЛІПІДІВ КСИЛОТРОФНИХ БАЗИДІОМІЦЕТІВ У ГЛИБИННІЙ КУЛЬТУРІ

2013 ◽  
Vol 3 (02) ◽  
pp. 220
Author(s):  
A. V. Chaika ◽  
O. V. Fedotov
Keyword(s):  
Lipid Peroxidation ◽  
Growth Rate ◽  
Culture Filtrate ◽  
Submerged Fermentation ◽  
Gravimetric Method ◽  
Correlation Coefficients ◽  
High Growth ◽  
Its Region ◽  
Thiobarbituric Acid ◽  
Xylotrophic Basidiomycetes

<p>The aim of the study was to determine the lipid peroxidation (LP) intensity of xylotrophic basidiomycetes cultures under submerged fermentation on glucose-peptone medium. The materials were submerged mycelium and culture filtrate of 79 strains of 18 species xylotrophic basidiomycetes. Among the studied strains, 60 strains belong to the order <em>Agaricales</em> and 19 – to order <em>Polyporales</em>. Most of the strains (85%) were isolated from the fruiting bodies collected in different localities of Donetsk city and its region. The following methods were used. Oven-dry biomass was determined by gravimetric method and biomass increase and specific growth rate were calculated. The pH of the culture filtrate was determined by potentiometric method. The intensity of lipid peroxidation processes was estimated with the modified thiobarbituric acid test. The study concluded that among the studied strains high level of LP in the mycelium is typical for the <em>F. velutipes</em> cultures, particularly the F-vv, F-03 and F-1. The low intensity of LP in the mycelium was established for the <em>F. fomentarius</em> Ff-09, Ff-1201 strains and the <em>L. edodes</em> Le-2, Le-4, Le-6, Le-7 strains. The high content of LP products in the culture filtrate is typical in most <em>Polyporales</em> cultures, for example, in the strains of <em>I. lacteus</em> IL-1201, <em>T. hirsuta</em> Th-11, <em>D. quercina</em> Dq-08 and <em>F. fomentarius</em> T-10. Paucity of the LP products in the culture filtrate established for some strains of <em>F. velutipes</em>, for example, F-204, F-11, <em>S. commune</em> Sc-1102 strain and <em>G. lucidum</em> cultures. Direct dependence between the content of LP products in the mycelium and culture filtrate was not established. Calculated correlation coefficients for the investigated strains showed the dependence of the growth rates and the intensity of LP in the mycelium and culture filtrate. Selected strains with high growth rate and a significant LP level in the culture filtrate are promising in technologies of lignocellulosic wastes and xenobiotics biodegradation and environment bioremediation.</p> <p><em>Key words: xylotrophic basidiomycetes, submerged fermentation, lipid peroxidation</em></p>

scholarly journals КУЛЬТУРАЛЬНО-МОРФОЛОГІЧНІ ХАРАКТЕРИСТИКИ КСИЛОТРОФНИХ БАЗИДІОМІЦЕТІВ У ГЛИБИННІЙ КУЛЬТУРІ

2013 ◽  
Vol 3 (01) ◽  
pp. 128
Author(s):  
A. V. Chaika ◽  
O. V. Fedotov
Keyword(s):  
Submerged Fermentation ◽  
Morphological Characteristics ◽  
Its Region ◽  
Submerged Cultivation ◽  
Submerged Cultures ◽  
Biosynthetic Activity ◽  
Xylotrophic Basidiomycetes ◽  
Weighing Method ◽  
Peptone Medium ◽  
Biomass Increase

<p>The aim of the present study was to evaluate the growth specificities of 79 strains of 18 species xylotrophic basidiomycetes under submerged fermentation on glucose-peptone medium. Most of the strains (85%) were isolated from the fruiting bodies collected in different localities of Donetsk and its region. The macro- and microscopic structure of pellets was studied, pH of the culture filtrate was determined by potentiometric method, ovendry biomass was determined by weighing method and biomass increase and specific growth rate were calculated. The results obtained allowed to ascertain the strain and species character of culture-morphological characteristics of the studied basidiomycetes. On the basis of the research the higher basidiomycetes submerged cultivation method was optimized making the period of fermentation significantly reduced. The growth peculiarities revealed can be used for further studies of biosynthetic activity of the xylotrophic basidiomycetes submerged cultures.</p> <p><em>Key words: xylotrophic basidiomycetes, submerged fermentation, growth and morphological characteristics</em></p>

scholarly journals The lipid peroxidation intensity of fungi strains from the orders Agaricales and Polyporales

2016 ◽  
Vol 24 (2) ◽  
Author(s):  
O. V. Fedotov
Keyword(s):  
Lipid Peroxidation ◽  
Culture Filtrate ◽  
Biomass Accumulation ◽  
Thiobarbituric Acid ◽  
Systematic Position ◽  
Metabolic Products ◽  
Dry Biomass ◽  
Weighing Method ◽  
Peptone Medium ◽  
Rates Of Growth

This article is devoted to investigation of the dynamics of growth and level of spontaneous and induced lipid peroxidation intensity of Basidiomycetes strains grown by surface cultivation on a glucose-peptone medium. The materials of the research are mycelium and culture filtrates (CF) of 57 strains (5 belong to 5 species from the order Polyporales s.l., and 52 belong to 7 species of the order Agaricales s.l.). To study the dynamics of growth we used a weighing method for determining the accumulation of absolutely dry biomass. Intensity of lipid peroxidation was determined by a modified spectrophotometric method for content of active to thiobarbituric acid products. It was found that the most productive in absolutely dry biomass accumulation were the strains Flammulina velutipes (Curt.: Fr.) Sing. F-610 and Pleurotus eryngii (DC.: Fr.) Quél. P-er. The level of spontaneous and induced LPO intensity in mycelia of all strains was higher than this figure in the culture filtrate and increased with the duration of cultivation. Dependencies between the content of lipid peroxidation products in the mycelia and CF were not established. The lowest values were recorded for biomass accumulation by the strains Pleurotus ostreatus (Jacq.: Fr.) P. Kumm. P-14, P-192 and P. citrinopileatus Singer. Р-сіtr. Groups of basidiomycete cultures with different levels of TBA-AP were identified. Spontaneous and induced intensivity of lipid peroxidation in all studied strains of mycelia was higher than the figure in the culture filtrate. The intensity of lipid peroxidation in both mycelia and culture filtrate constantly increased, which can be explained by the growing shortage of certain nutrients (primarily carbon) and increased concentration of metabolic products in the medium. The ratio of spontaneous and induced lipid peroxidation intensity is specific to each strain and is independent of its systematic position. Shifting of prooxidant-antioxidant balance to a relatively stationary level is a mark of stress reaction. LPO-products can be both inductors and primary mediators of stress as a special class of biological systems. Selected strains with high rates of growth and LPO ntensity are promising for applications in biotechnology and ecology.

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome

2011 ◽  
Vol 14 (3) ◽  
pp. 443-448 ◽  
Author(s):  
N. Kurhalyuk ◽  
H. Tkachenko ◽  
K. Pałczyńska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Brown Trout ◽  
Salmo Trutta ◽  
Thiobarbituric Acid ◽  
Control Group ◽  
Thiobarbituric Acid Reactive Substance ◽  
Tbars Level ◽  
Reactive Substance ◽  
Brown Trout Salmo Trutta

Resistance of erythrocytes from Brown trout (Salmo trutta m. trutta L.) affected by ulcerative dermal necrosis syndrome In the present work we evaluated the effect of ulcerative dermal necrosis (UDN) syndrome on resistance of erythrocytes to haemolytic agents and lipid peroxidation level in the blood from brown trout (Salmo trutta m. trutta L.). Results showed that lipid peroxidation increased in erythrocytes, as evidenced by high thiobarbituric acid reactive substance (TBARS) levels. Compared to control group, the resistance of erythrocytes to haemolytic agents was significantly lower in UDN-positive fish. Besides, UDN increased the percent of hemolysated erythrocytes subjected to the hydrochloric acid, urea and hydrogen peroxide. Results showed that UDN led to an oxidative stress in erythrocytes able to induce enhanced lipid peroxidation level, as suggested by TBARS level and decrease of erythrocytes resistance to haemolytic agents.

Exploring SiC Growth Limitation of Vapor-Liquid-Solid Mechanism when Using Two Different Carbon Precursors

2013 ◽  
Vol 740-742 ◽  
pp. 323-326
Author(s):  
Kassem Alassaad ◽  
François Cauwet ◽  
Davy Carole ◽  
Véronique Soulière ◽  
Gabriel Ferro
Keyword(s):  
Growth Rate ◽  
High Growth ◽  
High Growth Rate ◽  
Carbon Precursor ◽  
Growth Limitation ◽  
Vapor Liquid Solid ◽  
Partial Pressures ◽  
Vapor Liquid Solid Mechanism ◽  
Vls Growth ◽  
Vapor Liquid

Abstract. In this paper, conditions for obtaining high growth rate during epitaxial growth of SiC by vapor-liquid-solid mechanism are investigated. The alloys studied were Ge-Si, Al-Si and Al-Ge-Si with various compositions. Temperature was varied between 1100 and 1300°C and the carbon precursor was either propane or methane. The variation of layers thickness was studied at low and high precursor partial pressure. It was found that growth rates obtained with both methane and propane are rather similar at low precursor partial pressures. However, when using Ge based melts, the use of high propane flux leads to the formation of a SiC crust on top of the liquid, which limits the growth by VLS. But when methane is used, even at extremely high flux (up to 100 sccm), no crust could be detected on top of the liquid while the deposit thickness was still rather small (between 1.12 μm and 1.30 μm). When using Al-Si alloys, no crust was also observed under 100 sccm methane but the thickness was as high as 11.5 µm after 30 min growth. It is proposed that the upper limitation of VLS growth rate depends mainly on C solubility of the liquid phase.

scholarly journals Antioxidant and Antimicrobial Effect of Plant Essential Oils and Sambucus nigra Extract in Salmon Burgers

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 776
Author(s):  
Kristina Jonušaite ◽  
Petras Rimantas Venskutonis ◽  
Gines Benito Martínez-Hernández ◽  
Amaury Taboada-Rodríguez ◽  
Gema Nieto ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Essential Oils ◽  
Antioxidant Capacity ◽  
Control Sample ◽  
Thiobarbituric Acid ◽  
Antimicrobial Effect ◽  
Total Phenolic ◽  
Sambucus Nigra ◽  
Plant Essential Oils ◽  
Microbial Counts

The antioxidant capacity of oregano (OEO) and clove (CLEO) essential oils and black elderberry (Sambucus nigra) flower extract (SNE) were compared with butylhydroxytoluene (BHT) regarding its protection against lipid peroxidation and microbial counts in salmon burgers stored at 4 °C for 14 days and after cooking. The content of total phenols was 5.74% in OEO, 2.64% in CLEO and 2.67 % in the SNE. The total phenolic content and the antioxidant capacity were significantly higher (p < 0.05) for SNE and OEO. Both essential oils showed a similar IC50 and inhibition percentage of lipid peroxidation to BHT. The combination of OEO and SNE reduced 29% of thiobarbituric acid reactive substances (TBARS), while BHT reduced 31% of TBARS generated during refrigeration storage in salmon burgers in relation to the control sample without antioxidants. Additionally, the microbial counts after 14 days of refrigeration were the lowest in burgers when the combination of OEO and SNE was used. This study concludes that OEO and SNE can be used as inhibitors of lipid oxidation in salmon products and as natural candidates to replace commonly used synthetic antioxidants and antimicrobials in these food products.

scholarly journals Biochemical Studies on Phenylhydrazine Induced Experimental Anemic Albino Rats

2015 ◽  
Vol 3 (1) ◽  
pp. 41-47
Author(s):  
Nirjala Laxmi Madhikarmi ◽  
Kora Rudraiah Siddalinga Murthy
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Hydroperoxide ◽  
Thiobarbituric Acid ◽  
Albino Rats ◽  
Enzymatic Antioxidants ◽  
Medical Sciences ◽  
Thiobarbituric Acid Reactive Substances ◽  
Biochemical Studies ◽  
Healthy Control ◽  
Wistar Albino Rats

INTRODUCTION: The present study evaluated the modulatory effects of diphenylhydrazine induced experimental wistar albino rats and also to assess various biochemical parameters in whole blood and red blood cell lysate.MATERIALAND METHODS: Twenty male albino rats weighing 180-200 gm were selected for the study and divided in two groups; ten phenylhydrazine dihydrochloride (PHZ) induced anemia and ten healthy control. Thiobarbituric acid reactive substances and lipid hydroperoxide were measured as lipid peroxidation parameter. The antioxidant vitamins A, C and E and enzymatic antioxidants; catalase, glutathione peroxidase and superoxide dismutase were also assessed.RESULTS: Phenylhydrazine induced anemic rats showed a significant increase in the lipid peroxidation and decrease in the antioxidants as compared to healthy rats.CONCLUSION: The study concludes that phenylhydrazine induced experimental anemic albino rats showed increased oxidative stress than compared with healthy albino rats.Journal of Universal College of Medical Sciences Vol. 3, No. 1, 2015: 41-47 

Lipid Peroxidation in Hyperlipidaemic Patients. A Study of Plasma using an HPLC-Based Thiobarbituric Acid Test

1993 ◽  
Vol 19 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Susanna Chirico ◽  
Cheryl Smith ◽  
Christine Marchant ◽  
Malcolm J. Mitchinson ◽  
Barry Halliwell
Keyword(s):  
Lipid Peroxidation ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Test ◽  
Acid Test
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