scholarly journals Вплив різних рівнів кальцію та фосфору у комбікормах на мінеральний склад скелету японських перепелів

2017 ◽  
Vol 7 (2) ◽  
pp. 14-18
Author(s):  
L. M. Zlamanyuk
Keyword(s):  
Japanese Quail ◽  
Experimental Studies ◽  
Live Weight ◽  
Simultaneous Increase ◽  
Control Group ◽  
Dry Matter Content ◽  
Group 4 ◽  
Significant Difference ◽  
Calcium And Phosphorus ◽  
Different Levels

<p>The authors investigated the effect of different levels of calcium and phosphorus in the mixed fodder on mineral composition of Japanese quail skeleton. Experimental studies were carried out in the conditions of the Problematic Research Laboratory of Mixed Fodder Additives, National University of Life and Environmental Sciences of Ukraine (Kiev, Ukraine). We selected Japanese quail as the material for scientific and economic experience.</p><p>The experiment was conducted by groups-analogues method. Fodder was in the dry loose form. The difference in feeding was due to different levels of calcium and phosphorus in the feed for experimental groups. In the beginning of the experiment the experimental quail were similar regards live weight while in the end we did not detect significant difference in their mass. Nevertheless, we observed little decrease in live weight with increasing of phosphorus up to 1.4 % and calcium up to 3.5 %. We fixed that water content in the quail skeletons from Group 4, 5 and 6th study groups (calcium 4.0 %, phosphorus 0.8, 1,1 and 1,4 % respectively) were smaller in comparison with quails from Control, Group 2 and 3 (calcium content 3.5 % with the same level of phosphorus). We also determined increasing of dry matter content in the skeleton by 2.5 – 2.8 % while increasing the content of calcium and phosphorus in mixed fodder of quails-hens (Group 4, 5 and 6). The phosphorus content in quail skeleton depended on the level of phosphorus in the mixed fodder and can be up to 0.39-0.66 % in the bones. The simultaneous increase in the level of phosphorus and calcium in mixed fodder of experimental groups was accompanied by increase in the concentration of calcium and magnesium in the quail skeleton by 0.85–0.87 and 0.01–0.07 per cent respectively.</p>

scholarly journals Effect of feeding of chelated zinc form on security, productivity and slaughter parameters of broilers

2021 ◽  
pp. 110-118
Author(s):  
Tetiana Fotina ◽  
Hanna Fotina ◽  
Svitlana Nazarenko ◽  
Roman Tymoshenko ◽  
Oleksii Fotin
Keyword(s):  
Broiler Chickens ◽  
Average Daily Gain ◽  
Experimental Studies ◽  
Live Weight ◽  
Feed Consumption ◽  
Control Group ◽  
Significant Difference ◽  
Veterinary Faculty ◽  
Experimental Group ◽  
Effect Of Feeding

The results of the effect of feeding the chelated form of Zinc on the safety, productivity and slaughter parameters of broiler chickens are presented. The aim of this study was to determine the safety, productivity and slaughter parameters of broilers on the background of feeding chelated form of Zinc. Materials and methods. Experimental studies were conducted during 2020 on broiler chickens cross Cobb-500 in the vivarium of the Veterinary Faculty of Sumy National Agrarian University (Ukraine), divided into two groups on the principle of analogues (control and experimental) of 50 broilers each. The source of zinc for chickens in the control group was their sulfates. Chelated forms of Zinc were used for broiler chickens of the experimental group. All groups of broiler chickens were administered the compound with compound feed in a dose that corresponded to the daily requirement of the bird in this trace element. The duration of the experiment is 42 days. The growth and development of broilers were evaluated on the basis of determining the relevant zootechnical indicators. Live weight of birds was determined by individual weighing on scales of the VNC type with an accuracy of ±1 g at the age of 7, 14, 21, 28, 35 and 42 days. Both scientific and economic experiments also determined the preservation of livestock - daily by the number of culled and dead birds. Feed consumption was recorded daily, for each week of cultivation and for the entire period of the experiment. At the end of the experiment, the cost of feed per 1 kg of live gained weight was calculated. Results. According to the results of the conducted researches, it was established that feeding of the chelated form of Zinc probably increased both the average daily gain and the total live weight of broiler chickens at different age periods of rearing. The live weight of chickens at the beginning of the experiment was almost the same, but at 7 days of age it tended to increase in chickens of the experimental group, although a significant difference between groups in this indicator was not found. It was found that the weight of uneviscerated, semi-eviscerated and eviscerated chicken in poultry of the experimental group was greater than similar indicators in the young animals of the control group by 9.3, 9.2, and 9.8 %, and this difference was significant (p<0.01). The yield of semi-eviscerated carcasses was higher in ones of the experimental group (84.19 %), and the difference was significant (p<0.05). Conclusions. It was found that the live weight of chickens of the experimental group (consumption of feed with a chelated form of Zinc) prevailed at 7 days of age by 1.1 g; on the 14th day – on 22.1 g (p<0.01); at 21 days – 35.4 g (p<0.01); at 28 days – 94.5 g (p<0.05); at 35 days – 133.6 g (p<0.01); at 42 days – at 218.9 g (p<0.05). Preservation of young animals in the experimental group, which used chelated compounds of Zinc, was higher and amounted to 96 %, compared with the control – 92 %. By age, feed costs per unit weight gain were lower in the experiment, respectively: 0.05; 0.08; 0.12; 0.06; 0.07 and 0.05 kg, compared to the control group. Growing broiler chickens on compound feed with a chelated form of Zinc by 9.8 % (p<0.01) increases the weight of the eviscerated carcass.

scholarly journals The efficiency of growing rainbow trout (Oncorhynchus mykiss) at different levels of lysine and methionine in feeding

2021 ◽  
pp. 173-181
Author(s):  
V. Kondratiuk
Keyword(s):  
Rainbow Trout ◽  
Amino Acid ◽  
Experimental Studies ◽  
Feed Consumption ◽  
Control Group ◽  
Positive Trend ◽  
Economic Optimization ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Significant Difference ◽  
Different Levels

The article considers the issue of the effectiveness of the use of complete feed with different levels of lysine and methionine for the cultivation of rainbow trout. Experimental studies on this yearling rainbow trout є (Walbaum, 1792) were conducted in the farm «Shipot» Perechyn district of Transcarpathian region. The aim of the experiment was to establish the effect of different levels of amino acid nutrition of this yearling trout on its performance. For this purpose, five experimental groups were formed by the method of analogues. The experiment lasted 55 days and was divided into two periods: equalization (5 days) and basic (50 days). During the equalization period, the experimental fish consumed feed of the control group. In the basic period, the level of lysine and methionine in experimental feeds for different experimental groups of trout ranged from 2.7 to 3.1% and from 0.85 to 1.05%, respectively. Trout were fed 6 times a day during the growing season. The required amount of feed was calculated according to the indicators of individual weight of young and the temperature of the environment at the time of feeding. Trout were grown in ponds at a water level of 1 m. Weighing of experimental young trout was performed once every 5 days. It is proved that the reduction of lysine content to 2.7% and methionine to 0.85% in compound feed for this year trout weighing more than 10 g causes a significant (p<0.05) decrease in fish productivity. In particular, it was found that feeding compound feeds with the above amino acid levels helps to reduce trout weight by 10.4% and reduce growth rate by 9.1–19.9% compared to fish fed lysine and methionine at level 2.9 and 0.95%, respectively. The increase in these indicators in the feed for trout this year is accompanied by some positive trend to increase their productivity, but no significant difference has been established. In the course of research it was determined that the feed consumption per 1 kg of weight gain in this year of trout, which received feed with a content of lysine 3.0% and methionine 1.0% were lower by 10.3%, and with the content of these amino acids at level 2.7 and 0.85%, respectively — 17.1% higher compared to fish that consumed food with a content of lysine 2.9 and methionine 0.95%. The use of complete feed with different levels of lysine and methionine for feeding trout this year did not significantly affect the safety of fish, which was in the range of 82.0–83.1%. For the production of trout products in terms of maximum productivity and economic optimization criteria, for feeding trout weighing more than 10 g, it is recommended to use complete feed with a level of lysine — 3.0% and methionine — 1.0%.

scholarly journals ВПЛИВ РІЗНИХ РІВНІВ АРГІНІНУ НА ЗОТЕХНІЧНІ ТА ЗАБІЙНІ ПОКАЗНИКИ МОЛОДНЯКУ ПЕРЕПЕЛІВ

2017 ◽  
Vol 7 (1) ◽  
pp. 37-45 ◽  
Author(s):  
I. I. Ibatullin ◽  
A. M. Omelian ◽  
M. Yu. Sychov
Keyword(s):  
Average Daily Gain ◽  
Experimental Studies ◽  
Live Weight ◽  
Feed Additives ◽  
Control Group ◽  
Research Groups ◽  
National University ◽  
The Cost ◽  
Daily Gain ◽  
Different Levels

<p>The productivity of young meat quails was estimated at use of feed with different levels of arginine. Experimental studies were conducted in course of Problem Research Laboratory of feed additives of National University of Life and Environmental Sciences of Ukraine. Pharaoh quails were used for scientific experiment that was carried out by method of analog groups. Arginine level contamination in the diets of experimental groups was regulated by the introduction of feed synthetic analogs of amino acid. Part of arginine in mixed fodder of poultry control group was 1.57 % in the fodder of quails of 2-nd, 3-rd, 4-th and 5-th research groups and that were 1.39, 1.48, 1.66, 1.75 % respectively. The feeding of the was done by group and feed was distributed twice – in morning and evening. Ii was proved that the feeding of quails by mix fodder with arginine content of 1.66% contributes to increment of the live weight by 2.6 % on average, daily gain - by 2.7 %, the weight before slaughter by 3 % and reduce the cost of feed per 1 kg of growth by 1.5 %. This could contribute to the further research of arginine-lysine optimal ratio in mixed fodder for quails and their impact on zootechnical indices.</p>

scholarly journals Effect of feeding sows PVMA Intermiks on digestibility and metabolism of pigs

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
N. V. Lyubasyuk
Keyword(s):  
Live Weight ◽  
The Body ◽  
Control Group ◽  
Large White ◽  
Feed Digestibility ◽  
Significant Difference ◽  
Lactating Sows ◽  
Special Supplement ◽  
Calcium And Phosphorus ◽  
Weaning Piglets

Pork production in modern conditions associated with the use of feed and dietary supplements as part of diets, because without them to provide animals essential nutrients almost impossible. Especially for little Ingredient grain rations. Therefore have resorted to creating new PVMA. These concerns and PVMA Intermiks PS – 7.5% and Intermiks LS – 20% – according to gestation and lactating sows. So were conducting research on the influence of feeding PVMA Intermiks sows in different periods of the reproductive cycle of feed digestibility and metabolism of nitrogen, calcium and phosphorus in their piglets weaned at 28 days age. Research groups conducting two–analogues pigs of large white breed balance method, under which the pigs were kept in individual cages and accounted for eight days consumed feed, feces and urine color. The live weight per head was 9 kg. Sows experimental group during 85 days gestating 7.5% by weight was replaced grain mix PVMA Intermiks PS, and next to farrowing and weaning piglets at 28 days age, replaced 20% concentrated feed on PVMA Intermiks LS. Sows in the control group were kept in complete feed appropriate. After all born piglets up to 14 days eating a special supplement Intermiks SP «Bambino» – 25%, and from 14 to 28 days – Intermiks SP – 25%. Studies have shown that feeding sows PVMA Intermiks increases performance polycarpous 10.8% weight in the pig–breeding nests by 25.3% and 13.3% macrocarpa. The weight of a piglet at birth the control and the experimental groups was 1.05 and 1.19 kg and at weaning at 28 days of life – 8.52 and 9,1 kg. Piglets from sows receiving PVMA Intermiks had higher levels of digestibility of crude oil (3.3%) and fiber (to 5.37%). No significant difference was obtained for the digestibility of dry, organic matter and protein compared to piglets from sows with complete feed in the diet. In the experimental pigs tends to increase the number of nitrogen retained in the body (14.5 %) and from the accepted and digested (by 4.4 and 6.0%). Terms feeding lactating sows gestation and no likely impact on calcium and phosphorus in pigs weaned at 28 days age.

scholarly journals Optimizing Manufacturing and Osseointegration of Ti6Al4V Implants through Precision Casting and Calcium and Phosphorus Ion Implantation? In Vivo Results of a Large-Scale Animal Trial

Materials ◽  
2020 ◽  
Vol 13 (7) ◽  
pp. 1670 ◽  
Author(s):  
Wölfle-Roos JV ◽  
Katmer Amet B ◽  
Fiedler J ◽  
Michels H ◽  
Kappelt G ◽  
...  
Keyword(s):  
Ion Implantation ◽  
Large Scale ◽  
In Vivo Studies ◽  
Control Group ◽  
Implant Surface ◽  
Precision Casting ◽  
Pull Out ◽  
Significant Difference ◽  
Calcium And Phosphorus

Background: Uncemented implants are still associated with several major challenges, especially with regard to their manufacturing and their osseointegration. In this study, a novel manufacturing technique—an optimized form of precision casting—and a novel surface modification to promote osseointegration—calcium and phosphorus ion implantation into the implant surface—were tested in vivo. Methods: Cylindrical Ti6Al4V implants were inserted bilaterally into the tibia of 110 rats. We compared two generations of cast Ti6Al4V implants (CAST 1st GEN, n = 22, and CAST 2nd GEN, n = 22) as well as cast 2nd GEN Ti6Al4V implants with calcium (CAST + CA, n = 22) and phosphorus (CAST + P, n = 22) ion implantation to standard machined Ti6Al4V implants (control, n = 22). After 4 and 12 weeks, maximal pull-out force and bone-to-implant contact rate (BIC) were measured and compared between all five groups. Results: There was no significant difference between all five groups after 4 weeks or 12 weeks with regard to pull-out force (p > 0.05, Kruskal Wallis test). Histomorphometric analysis showed no significant difference of BIC after 4 weeks (p > 0.05, Kruskal–Wallis test), whereas there was a trend towards a higher BIC in the CAST + P group (54.8% ± 15.2%), especially compared to the control group (38.6% ± 12.8%) after 12 weeks (p = 0.053, Kruskal–Wallis test). Conclusion: In this study, we found no indication of inferiority of Ti6Al4V implants cast with the optimized centrifugal precision casting technique of the second generation compared to standard Ti6Al4V implants. As the employed manufacturing process holds considerable economic potential, mainly due to a significantly decreased material demand per implant by casting near net-shape instead of milling away most of the starting ingot, its application in manufacturing uncemented implants seems promising. However, no significant advantages of calcium or phosphorus ion implantation could be observed in this study. Due to the promising results of ion implantation in previous in vitro and in vivo studies, further in vivo studies with different ion implantation conditions should be considered.

scholarly journals Influence guanidinoacetic acid on the growth of young animals quails meat direction of productivity

2016 ◽  
Vol 18 (2) ◽  
Author(s):  
M. Sychov ◽  
H. Pryumak
Keyword(s):  
Average Daily Gain ◽  
Live Weight ◽  
Control Group ◽  
Average Growth ◽  
Absolute Increase ◽  
Relative Growth ◽  
Guanidinoacetic Acid ◽  
Relative Gains ◽  
Daily Gain ◽  
Different Levels

The effect of using different levels guanidinoacetic acid in compound feeds on the growth of young quails meat direction of productivity. It is proved that adding to the basic diet of different levels guanidinoacetic acid (0.06, 0.12, 0.18 %) increases live weight of quails on 3.9 – 13.5 g and improves the absolute, average daily and relative gains compared to a bird in the control group, which were fed the main diet. The data show that at the age of 42 days of live weight was the highest in the diet of quail which was administered 0.12% GAA and dominant control of 13.5 g However, in 21 days live weight of quail fed 0.06 and 0.12 % guanidinoacetic acid was greater than control respectively 9.2 and 7.6 grams (P < 0.01). In absolute increments quail, fed GAA prevailed bird control group. The largest absolute increase was in birds that consumed the main diet of GAA level of 0.12% and prevailed controls 6.5 g (P < 0.001). A similar situation was of average daily gain. Young animals that received feed containing 0.12% guanidinoacetic acid had the highest average growth, which exceeded 0.9 g (15.5%) bird control group. As for the relative performance increases here were different. The lowest relative growth observed in birds which were administered GAA 0.06%, it lagged behind the controls 0.1 g in the same period quail treated with 0.12 and 0, 18% guanidinoacetic acid superior control respectively 2 and 1.1 g.

scholarly journals Comparative evaluation of postoperative pain intensity after single-visit and multiple-visit retreatment cases: a prospective randomized clinical trial

2018 ◽  
Vol 21 (1) ◽  
pp. 26 ◽  
Author(s):  
Hatice Miray Uyan ◽  
Keziban Olcay ◽  
Mutlu Özcan
Keyword(s):  
Postoperative Pain ◽  
Repeated Measures ◽  
Control Group ◽  
Periapical Lesions ◽  
Single Visit ◽  
Group 4 ◽  
Multiple Visit ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2

<p><strong>Objective:</strong> This study was designed to evaluate postoperative pain after endodontic retreatment. <strong>Material and Methods:</strong> Asymptomatic, multi-rooted molar &amp; premolar teeth requiring retreatment with 2–5 mm periapical lesions were included. Seventy-eight teeth were randomly placed in four groups (n=20): single-visit (control, group 1), Ledermix (group 2), metronidazole, ciprofloxacin, minocycline mixture (group 3), calcium hydroxide (group 4). The postoperative pain was recorded using a VAS at 6, 12, 24, and 48 h after retreatment. Statistical evaluation was performed using Two-Way Repeated Measures ANOVA and Tukey test. <strong>Results:</strong> Mild pain occurred in 67.5%, moderate in 30%, and flare-ups in 2.5%, and there was a significant difference between the groups (p&lt;0.01). Significantly lower postoperative pain was observed in TAP and CaOH<sub>2 </sub>groups(p&lt;0.05). In the 6, 12, and 24 h intervals, there was a significant difference in the pain levels (p&lt;0.05). <strong>Conclusion:</strong> TAP and CaOH<sub>2</sub> are effective for reducing postoperative pain after retreatment.</p><p><strong>Keywords</strong></p><p>Intracanal medicaments; Multiple-visit; Postoperative pain; Retreatment; Single-visit.</p>

The quality of pork when included in the diet of the drug Permaite

2021 ◽  
pp. 3-11
Author(s):  
N. I. Kulmakova ◽  
L. B. Leontiev
Keyword(s):  
Dry Matter ◽  
Silicon Oxide ◽  
Experimental Studies ◽  
Live Weight ◽  
Biologically Active ◽  
Potassium Oxide ◽  
Control Group ◽  
Chuvash Republic ◽  
On Farm

It is well known that the growth rate of an animal depends on the level of feeding. However, the diets used in farms do not always meet the body’s needs for biologically active substances. In recent years numerous studies have been conducted to study the eff ect of natural and synthetic complex biologically active drugs on the productivity, metabolism of pigs and the quality of the products obtained. The imbalance or lack of certain nutrients in the diet reduces the productivity of animals, reduces the conversion of feed. The purpose of our research was to study the effect of the drug Permaite included in the diet of pigs on some indicators of the quality and safety of pork. Permaite is a standardized zeolite-containing trepel of the Chuvash Republic. Its chemical composition is (%): silicon oxide – 64,39, iron oxide – 3,25. aluminum oxide – 8,42, titanium oxide – 0,45, calcium oxide – 7,74, calcium carbonate – 14,7, magnesium oxide – 1,71, potassium oxide – 1,81, sodium oxide – 0,03, phosphorus pentoxide – 0,20 and other impurities – 11,9. Experimental studies have been conducted on 2 groups of pigs on rearing and subsequent fattening. Pigs of the experimental group have been received the main diet with the inclusion of the drug Permaite at a dose of 3 % to the dry matter of the main diet in the form of a fraction of 0,1–0,3 mm. The drug has been prescribed once a day in a mixture with compound feed for 60 days. The control group of pigs during this period was on farm diet without the inclusion of the drug. Upon reaching the live weight of 100–110 kg the pigs have been sent for slaughter. The results of the research allow us to conclude that the inclusion of the drug Permaite in the dose of 3 % to the dry matter of the main diet provides the increase in the nutritional, biological, energy value, technological properties and safety of pork.

scholarly journals Do cigarette and alcohol affect semen analysis?

2016 ◽  
Vol 88 (1) ◽  
pp. 56 ◽  
Author(s):  
Mehmet Zeynel Keskin ◽  
Salih Budak ◽  
Saim Gubari ◽  
Kalender Durmaz ◽  
Mehmet Yoldas ◽  
...  
Keyword(s):  
Alcohol Use ◽  
Semen Analysis ◽  
Threshold Value ◽  
Semen Parameters ◽  
Control Group ◽  
Cigarette Use ◽  
Group 4 ◽  
Semen Volume ◽  
Significant Difference ◽  
The Relationship

Objectives: There are a number of studies about the effect of cigarette and alcohol on semen parameters in the literature. There is not a consensus on the relationship between use of cigarette and alchol and semen parameters in those studies. The number of studies in which cigarette and alcohol use are evaluated together is limited. This study was aimed to analyze the effect of cigarette and/or alcohol use on semen parameters. Methods: In this prospective study, 762 patients who applied to an hospital urology polyclinic between January 2015 and March 2015 due to infertility, were questioned for alcohol and cigarette use in anamnesis. The remaining 356 patients were included in our study. Then, semen analysis of the patients was performed. The patients were divided into five groups according to cigarette use, into five groups according to alcohol use and into four groups according to cigarette and/or alcohol use. Significant differences were analyzed between the groups in terms of semen volume, semen concentration, total motility, forward motility and morphological (normality, head anomaly, neck anomaly, tail anomaly) values. Results: According to cigarette use, only in group 4 (who use more than 20 package-years cigarette) semen volume was significantly lower than the control group (Mann-Whitney U, p = 0.009). There was no significant difference in any of the other parameters and groups compared with the control group (Mann-Whitney U, p &gt; 0,05) Conclusion:According to our study, using more than 20 package- years cigarette decreases semen volume. The reason of this result might be the fact that the threshold value, from which the effect of cigarette and alcohol use on the semen parameters has to be determined.

Efficacy of Double Manual Cleaning Versus Automated Cleaning for Removal of Biofilm of Hinged Surgical Instruments

2020 ◽  
Vol 41 (S1) ◽  
pp. s518-s519
Author(s):  
Dayane Costa ◽  
Roel Castillo ◽  
Lillian Kelly Lopes ◽  
Anaclara Tipple ◽  
Honghua Hu ◽  
...  
Keyword(s):  
Protein Level ◽  
Positive Control ◽  
Surgical Instruments ◽  
Microbial Load ◽  
Positive Control Group ◽  
Control Group ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2

Objectives: To evaluate the efficacy of double manual cleaning (DMC) with enzymatic followed by alkaline detergent for removing biofilm on hinged surgical instruments compared to automated cleaning by the washer-disinfector. Methods: Biofilm of Staphylococcus aureus (ATCC 25923) was formed in vitro on hemostatic forceps (Fig. 1). Biofilm-covered forceps were rinsed in distilled water and subjected to one of the following cleaning regimes (n = 5 forceps each): Group 1 forceps were soaked in sterile water for 5 minutes. Group 2-DMC forceps were soaked in enzymatic detergent, brushed 5 times on each face, rinsed with filtrated water (0.2 µm), soaked in alkaline detergent, brushed 5 times each face, rinsed with filtrated water (0.2 µm), and dried with sterile cloth. For group 3-DMC plus hinge inner brushing (n = 5), the forceps were soaked in detergents and brushed as in group 2, including hinge inner brushing (2-mm lumen brush) (Fig. 1). In group 4 (automated cleaning in a washer/disinfector), forceps were prewashed, washed once, washed again, rinsed, thermally rinsed, and dried. After the treatments, forceps were evaluated for microbial load (counting of colony-forming units), residual protein (BCA protein assay kit), and biofilm (scanning electron microscopy). Results: There was no statistically significant differences between the microbial load and protein level contaminating the forceps subjected to DMC (group 2) and the positive control group. The DMC with hinge inner brushing group (group 3) and the automated cleaning group (group 4) demonstrated a significantly reduced microbial load: reduction averages of 2.8 log 10 (P = .038) and 7.6 log10 (P ≤ .001), respectively. The protein level remaining on the forceps also significantly decreased: 2.563 μg (P = .016) and 1,453 μg (P = .001), respectively, compared to the positive control group. There was no statistically significant difference between DMC with hinge inner brushing and automated cleaning (groups 3 and 4) for all of the tests performed. None of the cleaning methods completely removed biofilm and/or soil from the forceps hinge internal region (Fig. 1). Conclusions: Automated cleaning had the best efficacy for removing biofilm. However, DMC with hinge inner brushing was an acceptable alternative cleaning method for sterilizing service units with only manual cleaning available, as is the case in most low- and middle-income countries. Neither automated nor any manual cleaning regimes were able to completely remove biofilm and soil from the forceps hinged area, and the amount of protein left after automated and DMC plus hinge brushing was higher than the recommended. Cleaning is the most important step for the reprocessing of reusable medical devices; thus, efforts must be undertaken to improve cleaning in different social and economic realities and scenarios.Funding: This study was supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – CAPES.Disclosures: None

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