scholarly journals Electrochemical Methods for Evaluation of Antioxidant Properties of Propolis Extract Incorporated in Chitosan Nanoparticles

2021 ◽  
Vol 57 (4) ◽  
pp. 96-108
Author(s):  
Luminita Fritea ◽  
Paula Melania Pasca ◽  
Laurian Vlase ◽  
Ana-Maria Gheldiu ◽  
Liviu Moldovan ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Antioxidant Properties ◽  
Chitosan Nanoparticles ◽  
Differential Pulse ◽  
Electrochemical Methods ◽  
Propolis Extract ◽  
Vis Spectroscopy ◽  
Pulse Voltammetry ◽  
Nano Formulation

The main purpose of our study was to demonstrate the antioxidant properties of novel propolis nano-formulation, incorporated in spherical chitosan nanoparticles. The electrochemical methods (cyclic voltammetry and differential pulse voltammetry) were successfully applied to propolis extract and nanopropolis formulation, confirming that the content in phenolic acids and flavonoids is responsible for the antioxidant activity of propolis. The quantitative and qualitative results are also supported by high performance liquid chromatography (HPLC) and ultraviolet-visible (UV-Vis) spectroscopy.

scholarly journals PHYTOCHEMICAL AND ANTIOXIDANT COMPOSITION IN ACCESSIONS OF THE MANGABA ACTIVE GERMPLASM BANK

2021 ◽  
Vol 34 (1) ◽  
pp. 228-235
Author(s):  
PRISCILLA SANTANA SANTOS ◽  
LISIANE DOS SANTOS FREITAS ◽  
EVANDRO NEVES MUNIZ ◽  
JOSEFA GRASIELA SILVA SANTANA ◽  
ANA VERUSKA CRUZ DA SILVA
Keyword(s):  
Principal Component Analysis ◽  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
High Performance ◽  
Antioxidant Properties ◽  
Principal Component ◽  
Total Phenols ◽  
Germplasm Bank ◽  
Fruit Species ◽  
Hancornia Speciosa

ABSTRACT Mangaba (Hancornia speciosa Gomes) is a fruit species native to Brazil, belonging to the Apocynaceae family. Its cultivation is predominantly extractivist and its antioxidant properties have been recently reported. The objective of this study was to evaluate the bioactive compounds and antioxidant activity in fruits of accession from the Active Germplasm Bank of Embrapa Coastal Tablelands (BI, CA, LG, PR, PT and TC). The extracts were analyzed by high-performance liquid chromatography (HPLC), which identified chlorogenic acid (93.71 - 131.66 mg.100g-1), ferulic acid (0.85 - 2.27 mg.100g-1) and rutin (238.59 -442.94 mg.100g-1). The accessions CA and PR showed the highest values of total phenols (1179.39 and 1167.05 mg GAE.100g-1, respectively). The accessions CA and TC had the highest concentration of the major compound rutin (436.78 and 442.94 mg.100g-1, respectively). Antioxidant activity values ranged from 125.95 to 158.67 g.g-1 DPPH. Principal component analysis grouped the accessions into four groups, due to the genetic variability verified in previous studies. The results will be useful to guide actions of selection and future breeding program of the species.

scholarly journals Effect of Chitosan Nanoparticles Incorporated with Antioxidants from Salvia hispanica L. on the Amaranth Flour Films

2021 ◽  
Vol 60 (1) ◽  
Author(s):  
Gema Morales-Olán ◽  
María Antonieta Ríos-Corripio ◽  
Aleida Selene Hernández-Cázares ◽  
Placido Zaca-Morán ◽  
Silvia Luna-Suárez ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Tensile Strength ◽  
Water Vapor ◽  
Antioxidant Properties ◽  
Chitosan Nanoparticles ◽  
Water Vapor Permeability ◽  
Vapor Permeability ◽  
Salvia Hispanica ◽  
Amaranth Flour ◽  
Salvia Hispanica L

Research background. Amaranth flour (Amaranthus hypochondriacus) produces films with excellent barrier properties against water vapor, allowing food preservation, but the mechanical properties are poor versus to synthetic films. One strategy to improve these properties is the incorporation of nanoparticles. The particles can also serve as a vehicle for the addition of antioxidants agents into the films. The objective of this work was to optimize the formulation for preparation of amaranth flour films treated with antioxidant chia (Salvia hispanica L.) extract-loaded chitosan particles using RSM. Experimental approach. Chitosan nanoparticles with the extract were synthesized by ionic gelation, and the films were made by the casting method. Three independent variables were assigned: amaranth flour (4-6 %), glycerol (25-35 %), and chitosan nanoparticles loaded with the chia extract (0-0.75 %). We then evaluated the physical (thickness), mechanical (tensile strength, Young´s modulus, and elongation), barrier (water vapor permeability, moisture, and water solubility), and antioxidant properties of the films. The experimental results of the properties were analyzed using a Box-Behnken experimental design generating 15 runs with three replicates at the central point. Results and conclusions. Second and third order polynomial models were obtained from the ANOVA analysis of the evaluated responses, and high coefficients of determination were found (0.91-1.0). The films presented a water vapor permeability of 0.82-2.39·10-7 (g·mm)/(Pa·s·m2), a tensile strength of 0.33-1.63 MPa, and antioxidant activity of 2.24-5.65 %. The variables had different effects on the films: The glycerol negatively affected their properties, and the permeability values increased with amaranth flour concentration. The nanoparticles improved the mechanical, barrier, and antioxidant properties of the films versus films without nanosystems. The optimal formulation was 4 % amaranth flour, 25 % of glycerol, and 0.36 % of chitosan nanoparticles. The optimized films had better mechanical (1.62 MPa) properties, a low water vapor permeability value (0.91·10-7 (g·mm)/(Pa·s·m2)), and moderate antioxidant activity (6.43 %). Novelty and scientific contribution. The results show the effect of chitosan nanoparticles on the properties of amaranth flour films for the first time. The resulting equations are useful in the design of food packaging.

scholarly journals A comparative study of extraction techniques for maximum recovery of bioactive compounds from Ganoderma lucidum spores

2020 ◽  
Vol 49 (1) ◽  
Author(s):  
Diana Carolina Saavedra Plaza ◽  
Carlos Ricardo Soccol ◽  
Miguel Daniel Noseda ◽  
Valcineide Oliveira de Andrade Tanobe ◽  
Oranys Marin ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Antioxidant Activity ◽  
Comparative Study ◽  
Ganoderma Lucidum ◽  
Antioxidant Properties ◽  
Extraction Techniques ◽  
Microwave Exposure ◽  
Vis Spectroscopy ◽  
Spore Walls ◽  
Microwave Heat

This study aimed at evaluating effective methods for breaking the hard and insoluble spores of Ganoderma lucidum to recover functional biomolecules. Rupture techniques were evaluated such as manual maceration (RM), maceration with spheres of various materials (BR), and microwave exposure plus maceration with steel/chrome spheres (MBR1). Spore rupture was evaluated using UV-Vis spectroscopy, which showed vibrations of 2955, 1642, 1240, 1080 and 1746 cm-1 corresponding to changes in spore walls. The MBR1 extract contained the largest amounts of carbohydrates (19.80 mg.g-1 spores) and polyphenols (2.21 mg.g-1 spores), whereas the BR extract had higher antioxidant activity (57.22%Inb DPPH). The MBR1 and BR extracts contained 62.2 and 73.5% glucose, respectively. Both methods also involved significant extraction of carbohydrates and proteins. The best way to extract biomolecules from spore walls is to perform a microwave heat treatment and break the walls with steel/chrome spheres; this produces large quantities of carbohydrates with antioxidant properties.

scholarly journals Multivalent Lactose–Ferrocene Conjugates Based on Poly (Amido Amine) Dendrimers and Gold Nanoparticles as Electrochemical Probes for Sensing Galectin-3

Nanomaterials ◽  
2020 ◽  
Vol 10 (2) ◽  
pp. 203 ◽  
Author(s):  
Manuel C. Martos-Maldonado ◽  
Indalecio Quesada-Soriano ◽  
Luis García-Fuentes ◽  
Antonio Vargas-Berenguel
Keyword(s):  
Gold Nanoparticles ◽  
Surface Modification ◽  
Cardiac Remodeling ◽  
Building Blocks ◽  
Differential Pulse ◽  
Pamam Dendrimers ◽  
Titration Calorimetry ◽  
Vis Spectroscopy ◽  
Galectin 3 ◽  
Pulse Voltammetry

Galectin-3 is considered a cancer biomarker and bioindicator of fibrosis and cardiac remodeling and, therefore, it is desirable to develop convenient methods for its detection. Herein, an approach based on the development of multivalent electrochemical probes with high galectin-3 sensing abilities is reported. The probes consist of multivalent presentations of lactose–ferrocene conjugates scaffolded on poly (amido amine) (PAMAM) dendrimers and gold nanoparticles. Such multivalent lactose–ferrocene conjugates are synthesized by coupling of azidomethyl ferrocene–lactose building blocks on alkyne-functionalized PAMAM, for the case of the glycodendrimers, and to disulfide-functionalized linkers that are then used for the surface modification of citrate-stabilized gold nanoparticles. The binding and sensing abilities toward galectin-3 of both ferrocene-containing lactose dendrimers and gold nanoparticles have been evaluated by means of isothermal titration calorimetry, UV–vis spectroscopy, and differential pulse voltammetry. The highest sensitivity by electrochemical methods to galectin-3 was shown by lactosylferrocenylated gold nanoparticles, which are able to detect the lectin in nanomolar concentrations.

scholarly journals Studies of the Variability of Polyphenols and Carotenoids in Different Methods Fermented Organic Leaves of Willowherb (Chamerion angustifolium (L.) Holub)

2020 ◽  
Vol 10 (15) ◽  
pp. 5254
Author(s):  
Marius Lasinskas ◽  
Elvyra Jariene ◽  
Nijole Vaitkeviciene ◽  
Jurgita Kulaitiene ◽  
Katarzyna Najman ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Solid Phase ◽  
Antioxidant Properties ◽  
Hierarchical Cluster ◽  
Organic Production ◽  
Biologically Active ◽  
Total Phenolic ◽  
Total Carotenoids ◽  
Chamerion Angustifolium

The demand for organic production is increasing worldwide. The willowherb, grown in an organic way, contributes greatly to the idea of a healthier society and clean land. Willowherb is widespread in the world and has high polyphenols, carotenoids, and antioxidant properties. The purpose of this work was to investigate the influence of solid-phase fermentation (SPF) under different conditions on the variation of polyphenols and carotenoids in the organic leaves of willowherb. The leaves were fermented for different periods of time: 24, 48, and 72 h; and in aerobic and anaerobic conditions. The evaluation of polyphenols and carotenoids was completed using high-performance liquid chromatography (HPLC), and antioxidant activity was measured with spectrophotometric method. Hierarchical cluster analysis was used to describe differences in biologically active compounds between willowherb samples. The experiment showed that the highest quantities of total phenolic acids and flavonoids were determined after 24 h under aerobic SPF, but the amountof total carotenoids was higher after 72 h anaerobic SPF, compared to control. Not-fermented willowherb leaves had a lower antioxidant activity. compared to fermented leaves. In conclusion, SPF can be used to change polyphenol and carotenoid quantities in organic leaves of willowherb.

scholarly journals Five-Golden-Flowers Tea: Green Extraction and Hepatoprotective Effect against Oxidative Damage

Molecules ◽  
2018 ◽  
Vol 23 (9) ◽  
pp. 2216 ◽  
Author(s):  
Cai-Ning Zhao ◽  
Guo-Yi Tang ◽  
Qing Liu ◽  
Xiao-Yu Xu ◽  
Shi-Yu Cao ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Gallic Acid ◽  
High Performance ◽  
Antioxidant Properties ◽  
Dry Weight ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Total Phenolic ◽  
Herbal Tea ◽  
Antioxidant Power

The consumption of herbal teas has become popular in recent years due to their attractive flavors and outstanding antioxidant properties. The Five-Golden-Flowers tea is a herbal tea consisting of five famous edible flowers. The effects of microwave-assisted extraction parameters on the antioxidant activity of Five-Golden-Flowers tea were studied by single-factor experiments, and further investigated using response surface methodology. Under the optimal parameters (53.04 mL/g of solvent/material ratio, 65.52 °C, 30.89 min, and 500 W), the ferric-reducing antioxidant power, Trolox equivalent antioxidant capacity, and total phenolic content of the herbal tea were 862.90 ± 2.44 µmol Fe2+/g dry weight (DW), 474.37 ± 1.92 µmol Trolox/g DW, and 65.50 ± 1.26 mg gallic acid equivalent (GAE)/g DW, respectively. The in vivo antioxidant activity of the herbal tea was evaluated on alcohol-induced acute liver injury in mice. The herbal tea significantly decreased the levels of aspartate aminotransferase, total bilirubin, and malonaldehyde at different doses (200, 400, and 800 mg/kg); improved the levels of liver index, serum triacylglycerol, and catalase at dose of 800 mg/kg. These results indicated its role in alleviating hepatic oxidative injury. Besides, rutin, chlorogenic acid, epicatechin, gallic acid, and p-coumaric acid were identified and quantified by high performance liquid chromatography (HPLC), which could contribute to the antioxidant activity of the herbal tea.

Natural Compounds with Antioxidant Properties

2009 ◽  
Vol 415 ◽  
pp. 25-28 ◽  
Author(s):  
Tudor Lupascu ◽  
Gheorghe Duca ◽  
Maria Giurginca ◽  
Pavel Vlad ◽  
Lucian Lupascu ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Molecular Mass ◽  
Antioxidant Properties ◽  
Natural Compounds ◽  
High Molecular Mass ◽  
Minimum Concentration ◽  
Natural Substances ◽  
Vis Spectroscopy

Natural substances with polyphenolic structure exhibit remarkable antioxidant properties, being widely used in preventing various diseases. Among these, the oenotanins obtained from grapes seeds are proantocianidinic compounds with high molecular mass, water insoluble and as a result, rather difficult to be utilized. The aim of this paper was to produce their hydrosolubilization and the structure of the product resulted (enoxyl) has been investigated by MS, IR, UV-Vis spectroscopy. The antioxidant activity of oenotanins and enoxyl has been quantified by chemiluminescence technique, and the minimum concentration able to capture completely the free radicals has been also established.

scholarly journals Comparison of Ethanolic and Aqueous-Polyethylenglycolic Propolis Extracts: Chemical Composition and Antioxidant Properties

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Mindaugas Liaudanskas ◽  
Loreta Kubilienė ◽  
Vaidotas Žvikas ◽  
Sonata Trumbeckaitė
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Chemical Composition ◽  
Phenolic Acids ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Alternative Form ◽  
Food Preservatives ◽  
Propolis Extract

In recent years, particular attention has been paid to the natural antioxidants. Bee products, especially propolis, are characterized by multifunctional (antioxidant, anti-inflammatory, antibacterial, antiviral, and food preservative) effects and can be used for the development of functional food or food preservatives. Propolis extracts that are commonly produced are ethanolic; therefore, to certain groups of consumers, for example, children and alcohol sensitive group, their applicability is limited. The aim of this study was to develop alternative propolis from aqueous-polyethylenglycolic propolis extract (AQUA-PEG) and compare the chemical composition as well as antioxidant (radical-scavenging and reduction properties) activities to those of ethanolic propolis extract (EEP). Polyethylene glycol is quite a good solvent, which can be successfully used for the preparation of NEP. The total quantity of phenolic compounds identified in AQUA-PEG (400.36 µg/mL), prepared according to our technology, is very similar to that of EEP (433.53 µg/mL), whereas the amount of phenolic acids was greater by 1.31-fold in AQUA-PEG and of flavonoids was greater by 2.38-fold in EEP. The antioxidant activity depends on the method used: by applying the ABTS and CUPRAC methods, both extracts demonstrate similar antioxidant (antiradical and reducing) activity, whereas in the case of the DPPH and FRAP method, significantly higher antioxidant activity was detected in EEP. This should be taken by researchers into account especially when interpreting the results and drawing conclusions about the antioxidant activity of propolis extracts. On the basis of the results, AQUA-PEG, prepared by the developed technology, can be used as an alternative form to ethanolic propolis extract, since it contains a large quantity of antioxidants, namely, flavonoids and phenolic acids. We believe that nonethanolic propolis extract has the prospect of being applied for the development of functional foods in order to alleviate certain symptoms of oxidative stress or for the prevention of some oxidative-stress-related diseases.

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