Betel nut (Areca catechu L.) extract toxicity on the kidney and liver of white rats (Rattus norvegicus)

This research was conducted to prove the ability of betel nut extract (Areca catechu) in causingdegradation of sperm motility on rats (Rattus norvegicus) strain Wistar. This research used 2-3 monthsage, 200-250 g body weight of male white rats Rattus norvegicus strain Wistar. The rats were dividedinto 5 groups in equal number, 3 rats each group. They were a control group without treatment and 4treatment groups which were given different doses of betel nut extract for 7 days, i.e., 1, 2, 3, and 4 g/200g body weight respectively. The result showed that increasing doses of betel nut extract can result indegradation of sperm motility significantly (P0.05).Keywords: Areca catechu, Rattus norvegicus, sperm motility

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PENGARUH PEMBERIAN EKSTRAK BIJI PINANG (ARECA CATECHU L.) TERHADAP WAKTU PERDARAHAN PASCA EKSTRAKSI GIGI PADA TIKUS JANTAN WISTAR (RATTUS NORVEGICUS L.)

JURNAL ILMIAH SAINS ◽

10.35799/jis.15.2.2015.9572 ◽

2015 ◽

Vol 17 (1) ◽

pp. 129

Author(s):

Jane Wuisan ◽

Bernat Hutagalung ◽

Wellsy Lino

Keyword(s):

Treatment Group ◽

Rattus Norvegicus ◽

Bleeding Time ◽

Male Rats ◽

Control Group ◽

Betel Nut ◽

Areca Catechu ◽

Time In Treatment ◽

Teeth Extraction ◽

Wistar Male Rats

PENGARUH PEMBERIAN EKSTRAK BIJI PINANG (ARECA CATECHU L.) TERHADAP WAKTU PERDARAHAN PASCA EKSTRAKSI GIGI PADA TIKUS JANTAN WISTAR (RATTUS NORVEGICUS L.) ABSTRAK Biji pinang (Areca catechu L.) merupakan salah satu tanaman obat yang dikenal masyarakat dapat menghentikan perdarahan. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak biji pinang terhadap waktu perdarahan pasca ekstraksi gigi pada tikus jantan galur Wistar. Pada penelitian ini metode yang digunakan merupakan eksperimental. Ekstrak biji pinang didapatkan dengan cara maserasi dan penguapan menggunakan Rotary vacuum evaporator. Jumlah sampel sebanyak 24 ekor tikus jantan galur Wistar yang dibagi dalam 3 kelompok. Sampel penelitian kelompok perlakuan terdiri dari 8 ekor tikus jantan galur Wistar. Delapan sampel kelompok perlakuan diberi ekstrak biji pinang dosis 13,834 mg/200 g BB. Perhitungan dosis menggunakan tabel faktor konversi. Proses ekstraksi gigi tikus dilakukan setelah 4 jam pemberian ekstrak. Pemeriksaan waktu perdarahan dimulai saat gigi telah terlepas dari soket dengan melihat darah yang keluar sampai mulai terbentuknya bekuan darah pada soket. Perhitungan waktu perdarahan menggunakan stopwatch. Hasil penelitian menunjukkan rerata waktu perdarahan kelompok perlakuan yaitu 0.6125 (menit.detik). Rerata waktu perdarahan kelompok perlakuan tidak jauh berbeda dengan rerata waktu perdarahan kelompok kontrol positif yang diberi transamin yaitu 0.3475 (menit.detik) dan lebih singkat dari kelompok kontrol negatif etanol 96% dengan rerata waktu perdarahan 1.79 (menit.detik). Penelitian yang telah dilakukan menyimpulkan terdapat pengaruh pemberian ekstrak biji pinang (Areca catechu L.) dosis 13,834 mg/200 g BB terhadap waktu perdarahan pasca ekstraksi gigi pada tikus jantan galur Wistar. Kata kunci: Biji pinang, waktu perdarahan, ekstraksi gigi THE EFFECT OF BETEL NUT (Areca catechu L.) EXTRACT ON BLEEDING TIME AFTER EXTRACTION IN WISTAR MALE RATS (Rattus norvegicus L.) ABSTRACT The betel nut (Areca catechu L.) is a medicinal plant known by society to halt bleeding. This study was aimed to know the effect of betel nut extract administration on bleeding time after teeth extraction in Wistar male rats. This study was an experimental study. Betel nut extracts were obtained by maceration and evaporation using Rotary vacuum evaporator. The number of samples were 24 Wistar male rats divided into three groups. The treatment group consisted of 8 Wistar male rats. Eight samples of treatment group were fed with betel nut extract at dosage of 13,834 mg/200 g body weight. Dosages were calculated using conversion factor table. The rats teeth were extracted 4 hours after betel nut extract administration. Evaluation of bleeding time was started from the loss of extracted teeth from socket by observing the shed blood to the formation of blood clots in the socket. The bleeding time was calculated using stopwatch. Study results indicated that average bleeding time in treatment group namely 0.6125 (minute.second). The average bleeding time in treatment group did not differ significantly from average bleeding time in positive control group fed with transamine namely 0.3475 (minute.second), and shorter from negative control group fed with 96% ethanol with average bleeding time of 1.79 (minute.second). This study concluded that there was an effect of betel nut extract (Areca catechu L.) administration at dosage of 13,834 mg/200 g body weight on bleeding time after teeth extraction in Wistar male rats. Keywords: Betel nut, bleeding time, teeth extraction

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THE HIGH DOSE TOXICITY OF BETEL NUT (Areca catechu L.) ON REPRODUCTION ORGAN OF RATS

JAMBI MEDICAL JOURNAL Jurnal Kedokteran dan Kesehatan ◽

10.22437/jmj.v7i1.7059 ◽

2019 ◽

Vol 7 (1) ◽

pp. 41-49

Author(s):

Ave Olivia Rahman ◽

Herlambang Herlambang ◽

Charles A Simanjuntak ◽

Hasna Dewi ◽

Ahmad Syauqy ◽

...

Keyword(s):

Rattus Norvegicus ◽

Histopathological Examination ◽

Herbal Medicines ◽

Reproductive Organs ◽

Control Group ◽

High Dose ◽

Betel Nut ◽

Sprague Dawley ◽

Treatment Groups ◽

Areca Catechu

ABSTRACT Background : The use of long-term herbal medicines and high doses can damage organs, including the reproductive organs. Betel nut (Areca catechu L.) is one of the herbal ingredients that is consumed as a stamina enhancing beverage. The purpose of this study was to determine the effect of longterm treatment of raw betel nut at a dose of 10,000 mg/kg on testicular and ovary tissue of rats. Rats were Rattus norvegicus, Sprague Dawley strain, 2-3 months. Methods : Tweenty rats divided into 2 groups, each groups were 5 male and 5 female. Control group was given aquades and the treatment group was given raw betel nut with a dose of 10,000 mg/kgBW for 45 days with a gastrictube. Histopathological examination with Haematoxylin-Eosin staining was used to assses testicular and ovary tissues. Results: The ovaries of treatment groups had significantly lower de graff follicle compared to the control group (p < 0,05). The testis of treatment groups had significantly smaller diameter of tubulus seminifery, significantly higher necrosis of spermatogonia and spermatosit (p<0,05). Conclusion: Treatment of raw betel nut dose 10,000 mg /kgBW along 45 days causes damage of testicular and ovary tissues of rats.Keywords: Betel Nut, Areca Catechu L., Histopathology, Testicular, Ovary, Herbal Toxicity ABSTRAK Latar Belakang: Pemakaian obat herbal jangka panjang dan dosis tinggi dapat menyebabkan kerusakan organ tubuh, termasuk organ reproduksi. Biji pinang (Areca catechu L.) merupakan salah satu bahan herbal yang dikonsumsi sebagai minuman penambah stamina.Tujuan penelitian ini adalah untuk mengetahui pengaruh pemberian biji pinang muda dosis 10.000 mg/ kgBB selama 45 hari terhadap gambaran histopatologis testis dan ovarium tikus. Metode : Tikus yang digunakan adalah Rattus norvegicus galur Spague Dawley, usia 2-3 bulan, sebanyak 10 ekor jantan dan 10 ekor betina yang dibagi menjadi 2 kelompok, yaitu kelompok kontrol diberikan aquades dan kelompok perlakuan diberikan biji pinang dosis 10.000 mg/ kgBB dengan sonde. Pemeriksaan histopatologi dengan pewarnaan Haematoxylin-Eosin untuk organ testis dan organ ovarium. Hasil: Jumlah folikel de draf pada kelompok perlakuan lebih sedikit dibandingkan kelompok kontrol (p<0,05). Persentase nekrosis spermatogonia dan spermatosit pada kelompok perlakuan lebih tinggi dibandingkan kelompok kontrol (p<0,05), diameter tubulus seminifeus pada kelompok perlakuan juga lebih kecil dibandingkan kelompok kontrol (p<0,05). Kesimpulan: Pemberian biji pinang 10.000 mg/kgBB selama 45 hari menyebabkan kerusakan pada jaringan testis dan ovarium pada tikus. Kata Kunci : Biji Pinang, Areca Catechu L, Histopatologi, Ovarium, Testis, Toksisitas herbal

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Exploiting crude betel nut (Areca catechu Linn.) extracted solution as a natural reagent with sequential injection spectrophotometry for iron analysis in rice samples

Journal of the Iranian Chemical Society ◽

10.1007/s13738-021-02337-2 ◽

2021 ◽

Author(s):

Bordin Weerasuk ◽

Sam-ang Supharoek ◽

Kate Grudpan ◽

Kraingkrai Ponhong

Keyword(s):

Betel Nut ◽

Sequential Injection ◽

Areca Catechu ◽

Rice Samples ◽

Iron Analysis

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THE EFFECT OF NONI (Morinda citrifolia) EXTRACT TO HEPAR HISTOPATHOLOGY AND TRIGLYCERIDE LEVEL IN RATS (Rattus Norvegicus) INDUCED BY DISLIPIDEMIA

JURNAL AGRI-TEK Jurnal Penelitian Ilmu-Ilmu Eksakta ◽

10.33319/agtek.v20i2.34 ◽

2019 ◽

Vol 20 (2) ◽

pp. 76-81

Author(s):

Jhouharotul Faradisah ◽

Diah Purwaningsari

Keyword(s):

Lipid Metabolism ◽

Triglyceride Level ◽

Rattus Norvegicus ◽

Morinda Citrifolia ◽

Control Group ◽

High Cholesterol Diet ◽

Cholesterol Diet ◽

High Cholesterol ◽

White Rats ◽

Abnormal Lipid Metabolism

Dyslipidemia is an abnormal lipid metabolism which may cause fat degeneration on hepatocytes cells and elevated triglyceride serum level. Dyslipidemia can be prevented by the consumption of high antioxidant food. Noni(Morinda citrifolia) contains many antioxidant such as flavanoid, kuersetin, tannin, and saponin, which are able to prohibit the elevation of ROS.This research is aimed to find out the effect of noni(Morinda citrifolia) extract in reducing the number of hepatocyte’s cells with fat degeneration and decreasing the triglyceride level which is elevated due to high cholesterol diet induction.In this study white rats divided randomly into 4 groups, control group (K-), high cholesterol diet induced group (K+), high cholesterol diet induced with 100 mg/Kg BW noni extract group (P1), high cholesterol diet induced with 200 mg/Kg BW noni extract group (P2). The result shows that noni extract with dose 100 mg/Kg BW and 200 mg/Kg BWcan reduce the number of hepatocytes cells with fat degeneration (p= 0,026 and p=0,027) and decrease the level of triglyceride serum (p=0,036 and p=0,010).The conclusion is noni extract with dose 100 mg/KgBW reduces effectively the number of hepatocyte’s cells with fat degeneration and decreases the level of triglyceride serum which increase because of high cholesterol diet.

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PENGARUH PEMBERIAN INFUSA DAUN LABU SIAM (Sechium edule) TERHADAP PENURUNAN KADAR KOLETEROL DARAH TOTAL TIKUS PUTIH JANTAN (Rattus norvegicus)

PHARMACON ◽

10.35799/pha.9.2020.27422 ◽

2020 ◽

Vol 9 (1) ◽

pp. 151

Author(s):

Ausich Singal ◽

Edwin De Queljoe ◽

Paulina Yamlean

Keyword(s):

Rattus Norvegicus ◽

Dose Group ◽

T Test ◽

Blood Cholesterol ◽

Control Group ◽

Cholesterol Levels ◽

White Rats ◽

Sechium Edule ◽

One Way Anova ◽

Paired T Test

ABSTRACT This study aims to determine the effect of infusion of conjoined pumpkin leaves (Sechium edule) on reducing total blood cholesterol of male white rats (Rattus norvegicus). The subjects of this study were 15 male white rats with an average body weight of 200 grams which were divided into 5 groups, each group consisted of 3 mice. The method used is a laboratory experiment with a completely randomized design. The results were obtained from 2 measurements of blood cholesterol levels, namely measurements before and after treatment. The treatment begins with the provision of high-fat foods for 48 days. On the 49th day a blood cholesterol level was measured before treatment. Furthermore, treatment was given to each group, namely aquades in the negative control group, simvastatin in the positive control group, and squash leaves infusion with their respective doses in the dose group I (40%), the dose group II (20%), and the dose group III (10%). Measurement of cholesterol levels after treatment was carried out on day 54. Data were analyzed by Paired t-test and One Way ANOVA. The analysis showed that there were no significant differences between treatment groups. Judging from the change in average and percentage, 40% infusion dose of siamese pumpkin leaves gives the best reduction in cholesterol levels. Keywords: Cholesterol, pumpkin leaves, male white mouse infusion. ABSTRAK Penelitian ini bertujuan untuk mengetahui pengaruh pemberian infusa daun labu siam (Sechium edule ) terhadap penuruan kolesterol darah total tikus putih jantan (Rattus norvegicus). Subjek penelitian ini berupa tikus putih jantan berjumlah 15 ekor dengan berat badan rata-rata 200 gram yang dibagi dalam 5 kelompok, masing-masing kelompok sebanyak 3 ekor. Metode yang digunakan yaitu eksperimen laboratorium dengan rancangan acak lengkap. Hasil penelitian diperoleh dari 2 kali pengukuran kadar koleterol darah yaitu pengukuran sebelum dan sesudah perlakuan. Perlakuan dimulai dengan pemberian makanan tinggi lemak selama 48 hari. Pada hari ke49 dilakukan pengukuran kadar kolesterol darah sebelum perlakuan. Selanjutnya diberikan perlakuan pada tiap kelompok yaitu aquades pada kelompok kontrol negatif, simvastatin pada kelompok kontrol positif, dan infusa daun labu siam dengan dosis masing-masing pada kelompok dosis I (40 %), kelompok dosis II (20 %), dan kelompok dosis III (10%). Pengukuran kadar kolesterol sesudah perlakuan dilakukan pada hari 54. Data diananlisis dengan Paired t-test dan One Way ANOVA. Hasil analisa menunjukan tidak terdapat perbedaan yang bermakna antar kelompok perlakuan. Dilihat dari perubahan rerataan dan presentase, dosis infusa 40% daun labu siam memberikan penurunan kadar koleterol terbaik. Kata kunci : Infusa daun Labu siam, kolesterol, tikus putih jantan.

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UJI ANTIDIABETIK EKSTRAK ETANOL BUNGA PEPAYA (Carica papaya L.) TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽

10.35799/pha.9.2020.27423 ◽

2020 ◽

Vol 9 (1) ◽

pp. 160

Author(s):

Adinda Fransisca Pongoh ◽

Edwin De Queljoe ◽

Henki Rotinsulu

Keyword(s):

Blood Glucose ◽

Rattus Norvegicus ◽

Carica Papaya ◽

Fasting Blood Glucose ◽

Ethanol Extract ◽

Control Group ◽

Homogeneity Test ◽

Glucose Levels ◽

White Rats ◽

Blood Glucose Levels

ABSTRACT This study aims to determine the antidiabetic activity of papaya flower ethanol extract (Carica papaya L.) against male white rats (Rattus norvegicus) induced by alloxan. This research is experimental. Fifteen rats were divided into 5 treatment groups, each group consisted of 3 rats. The first group was the negative control group given Aquades, the second group was positive control given Glibenklamid, the three groups were 200 mg dose variation groups, four groups were 400 mg dose variations, and the five groups were 800 mg dose variations. Previously, rats were examined fasting blood glucose levels, then mice were induced by an alloxan dose of 120 mg / kgBW intraperitoneally. On the 3rd day blood glucose levels were examined and then treated according to groups for 7 days, measurement of blood glucose levels after the treatment was carried out once every 3 days namely day 3, day 7, and day 10. Data obtained were then analyzed statistically using SPSS , including normality test (Shapiro-Wilk), homogeneity test (Levene), ANOVA test (One way). The results of this study indicate that Papaya Flower (Carica papaya L.) Ethanol Extract can provide the best blood glucose level reduction effect at a dose of 800 mg. Keywords : Antidiabetic, Papaya Flower Extract (Carica papaya L.), Diabettes Mellitus, Male White Rat (Rattus norvegicus), Aloxan. ABSTRAK Penelitian ini bertujuan untuk mengetahui efektivitas Antidiabetik Ekstrak Etanol Bunga Pepaya (Carica papaya L.) Terhadap Tikus Putih Jantan (Rattus norvegicus) Yang Diinduksi Aloksan. Penelitian ini bersifat eksperimental. Sebanyak 15 ekor tikus dibagi ke dalam 5 kelompok perlakuan masing- masing kelompok terdiri dari 3 ekor tikus. Kelompok pertama kelompok Kontrol negatif yang diberikan Aquades, Kelompok kedua Kontrol Positif yang diberikan Glibenklamid, kelompok tiga kelompok variasi dosis 200 mg, kelompok empat kelompok variasi dosis 400 mg, dan kelompok lima kelompok variasi dosis 800 mg. Sebelumnya tikus dilakukan pemeriksaan kadar glukosa darah puasa, selanjutnya tikus diinduksi Aloksan dosis 120 mg/kgBB secara intraperitoneal. Pada hari ke 3 diperiksa kadar Glukosa darah kemudian diberikan perlakuan sesuai kelompok selama 7 hari, pengukuran kadar glukosa darah setelah perlakuan dilakukan 3 hari sekali yaitu hari ke 3, hari ke 7, dan hari ke 10. Data yang diperoleh kemudian dianalisis secara statistik menggunakan SPSS, meliputi uji normalitas (Shapiro-Wilk), uji homogenitas (Levene), uji ANOVA (One way). Hasil penelitian ini menunjukkan bahwa Ekstrak Etanol Bunga Pepaya (Carica papaya L.) dapat memberikan efek penurunan kadar glukosa darah yang paling baik yaitu pada dosis 800 mg. Kata kunci : Antidiabetik, Ekstrak Bunga pepaya (Carica papaya L.), Diabettes Mellitus, TikusPutih Jantan (Rattus norvegicus), Aloksan.

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PENGARUH GELATIN IKAN PATIN (PANGASIUS DJAMBAL) TERHADAP JUMLAH FIBROBLAS PADA LUKA PASCA PENCABUTAN GIGI TIKUS PUTIH (RATTUS NORVEGICUS)

ODONTO Dental Journal ◽

10.30659/odj.6.1.1-5 ◽

2019 ◽

Vol 6 (1) ◽

pp. 1

Author(s):

Fredy Mardiyantoro ◽

Fidya Fidya ◽

Dena Savira Andriani

Keyword(s):

Rattus Norvegicus ◽

Tooth Extraction ◽

Control Group ◽

Fish Gelatin ◽

Tukey Test ◽

White Rats ◽

Significant Difference ◽

Post Hoc ◽

Hematoxylin Eosin ◽

Test Result

Background: Gelatin has been used as a hemostatic agent to stop bleeding and it can be absorbed by human body within 6 hours. The patin fish (Pangasius djambal) gelatin has a high amount of amino acid, especially glutamine which has an important role in increasing the proliferation of fibroblast in wounds. The purpose of this study was to determine the effect of patin fish gelatin to the number of fibroblast in wound after tooth extraction.Method: Rat’s tooth extraction is done on the lower left incisive tooth. Thirty white rats were divided into two groups randomly with 3 time series. Control group was the rats without given of patin fish gelatin and treatment group was the rats given 1cc of patin fish gelatin with pipette. Socket taken for histologically processed by Hematoxylin Eosin staining.Result: It showed there were significant differences in the number of fibroblasts between groups. The Post Hoc Tukey test result showed there was a significant difference between the control group and the treatment group.Conclusion: Patin fish gelatin has effect increasing the number of fibroblast inwound after white rat’s (Rattus norvegicus) tooth extraction

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The Effect of Balimo (Zanthoxylum nitidum) Immersion Water On The Hematological Profile of White Rats (Rattus norvegicus) That Given Liquor (Ciu)

Biology Medicine & Natural Product Chemistry ◽

10.14421/biomedich.2021.101.47-57 ◽

2021 ◽

Vol 10 (1) ◽

pp. 47-57

Author(s):

Panca Buana Wijaya ◽

Tyas Rini Saraswati ◽

Silvana Tana ◽

Sunarno Sunarno ◽

Erma Prihastanti

Keyword(s):

Rattus Norvegicus ◽

Male Rats ◽

Excessive Alcohol Consumption ◽

White Rats ◽

Excessive Alcohol ◽

The Mean ◽

Zanthoxylum Nitidum ◽

The Difference ◽

Antioxidant Effects ◽

One Way Anova

Consumption of liquor such as Ciu in excessive doses can cause a decrease in hematological status. Balimo stem is an alternative treatment to improve hematological status due to excessive alcohol consumption because it contains alkaloids, flavonoids, and other secondary metabolic compounds, that have functions as antioxidant effects. This study aims to examine and analyze the effect of Balimo immersion water on the hematological status of mice with the observed variables, namely the erythrocytes count, hemoglobin levels, hematocrit value, and total count of leukocytes in rats that had been given Ciu. The study used 20 Rattus norvegicus male rats which were divided into 4 groups. The data were analyzed using one-way ANOVA. The results showed no significant differences (p <0.05) on the Balimo immersion water treatment, but if it was seen from the difference in the mean data of each variable, it could still be seen the difference from each treatment. In this study, it can be concluded that Balimo immersion water was able to improve the hematological status of rats that had been given Ciu liquor with a 0,2 mL dose.

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Identification of Potential Plants Producing Tannin-protein Complex for a-amylase as Botanical Pesticide

Pelita Perkebunan (a Coffee and Cocoa Research Journal) ◽

10.22302/iccri.jur.pelitaperkebunan.v29i1.189 ◽

2013 ◽

Vol 29 (1) ◽

Author(s):

Asriyah Firdausi ◽

Tri Agus Siswoyo ◽

Soekadar Wiryadiputra

Keyword(s):

Protein Complex ◽

Protein Complexes ◽

Gliricidia Sepium ◽

In Vitro Test ◽

Betel Nut ◽

Botanical Pesticides ◽

Botanical Pesticide ◽

Areca Catechu ◽

Gnetum Gnemon

Research on the development of botanical pesticides should be developed through new methods, such as by inhibiting the activity of digestive enzymes by secondary metabolites. The aim of this study was to identify some of potential plants as a source of tannin-protein complexes to inhibitthe activity of - amylase. The study of identification of potential plants producing the active ingredient tannin-protein complex was divided into three stages, 1) identification of potential plants producing tannin, 2) isolation of tannin-protein complexes, and 3) in vitro test of tannin-protein complexes effect of the -amylase activity. Some of the observed plants were sidaguri leaf (Sida rhombifolia), melinjo leaf (Gnetum gnemon), gamal leaf (Gliricidia sepium),lamtoro leaf (Leucaena leucocephala) , betel nut (Areca catechu) , and crude gambier (Uncaria gambir) a s a source of tannins and melinjo seed was used asprotein source. Betel nut and melinjo seed were the best source of tannin-protein complex, tannin content 1.77 mg TAE/mL with antioxidant activity of 90%,the ability to inhibit the activity of -amylase by 95% with IC 50 values of 10 mg/mL.Key words: Tannin, protein, -amylase, botanical pesticides,Areca catechu, Gnetum gnemon.

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