scholarly journals PENGARUH GELATIN IKAN PATIN (PANGASIUS DJAMBAL) TERHADAP JUMLAH FIBROBLAS PADA LUKA PASCA PENCABUTAN GIGI TIKUS PUTIH (RATTUS NORVEGICUS)

2019 ◽  
Vol 6 (1) ◽  
pp. 1
Author(s):  
Fredy Mardiyantoro ◽  
Fidya Fidya ◽  
Dena Savira Andriani
Keyword(s):  
Rattus Norvegicus ◽  
Tooth Extraction ◽  
Control Group ◽  
Fish Gelatin ◽  
Tukey Test ◽  
White Rats ◽  
Significant Difference ◽  
Post Hoc ◽  
Hematoxylin Eosin ◽  
Test Result

Background: Gelatin has been used as a hemostatic agent to stop bleeding and it can be absorbed by human body within 6 hours. The patin fish (Pangasius djambal) gelatin has a high amount of amino acid, especially glutamine which has an important role in increasing the proliferation of fibroblast in wounds. The purpose of this study was to determine the effect of patin fish gelatin to the number of fibroblast in wound after tooth extraction.Method: Rat’s tooth extraction is done on the lower left incisive tooth. Thirty white rats were divided into two groups randomly with 3 time series. Control group was the rats without given of patin fish gelatin and treatment group was the rats given 1cc of patin fish gelatin with pipette. Socket taken for histologically processed by Hematoxylin Eosin staining.Result: It showed there were significant differences in the number of fibroblasts between groups. The Post Hoc Tukey test result showed there was a significant difference between the control group and the treatment group.Conclusion: Patin fish gelatin has effect increasing the number of fibroblast inwound after white rat’s (Rattus norvegicus) tooth extraction

scholarly journals PENYEMBUHAN LUKA BAKAR PADA TIKUS PUTIH DENGAN MENGGUNAKAN EKSTRAK DAUN PEGAGAN (CENTELLA ASIATICA) 25% DAN EKSTRAK DAUN PETAI CINA (LEUCAENA LEUCOCEPHALA) 30%

2018 ◽  
Vol 10 (2) ◽  
Author(s):  
Syaifuddin Kurnianto ◽  
Kusnanto Kusnanto ◽  
Padoli Padoli
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Control Group ◽  
Test Duration ◽  
Moderate Degree ◽  
White Rats ◽  
Significant Difference ◽  
Post Hoc

Burns are one of the injuries that require the best care to achieve optimal cure. Several previous studies have suggested that leaf extract of Centella asiatica and chinese petai leaf potential as burning healing agent for mild and moderate degree besides medical treatment. The purpose of this study was to explain the difference of effectiveness of leaf extract of pegagan leaves 25% by giving 30% petai chinese extract to heal burns. The samples were white rats (Rattus norvegicus) with total of 28 tails selected according to inclusion criteria then divided into 4 groups randomly is group of leaf extract of pegagan leaf 25% (K1), 30% petai petroleum extract (K2) Gel base (K3) and control (K4). The design of this study used the randomized posttest only control group design and analyzed using Anova and post Hoc Duncan test. The measured variable is the duration of wound healing. Anova test results and Post Hoc Duncan test duration of burn wound healing show that 30% Chinese petai extract group has significant difference with other group with p-value <0,05. Treatment of burns by using chinese petroleum extract 30% more effective than 25% of pegagan leaf extract in healing burns in white rats (Rattus norvegicus) as evidenced by the fastest healing time of burns.

scholarly journals Effect of Giving Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract to Epithelialization In Incision Wound of White Rats (Rattus Norvegicus)

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Idola Perdana Sulistyoning Suharto
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Male Rats ◽  
Control Group ◽  
P Value ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference ◽  
Phaleria Macrocarpa ◽  
Incision Wound

The research purpose was to analysis effect of giving mahkota dewa fruits (Phaleria macrocarpa) extract to epithelialization in incision wound of white rats (Rattus norvegicus). The method was randomized posted-only control group design. There were 30 male rats (Rattus norvegicus) grouped on control and treatment group. Control group divided into three groups (KK1, KK2, KK3) and also treatment group divided into three groups (KP1, KP2, KP3). Control group just given CMC 1% peroral without mahkota dewa fruits extract, the treatment group given mahkota dewa fruits extract 22.5 mg/kg body weight. The data was analyzed by Kruskall Wallis. Based on Kruskall Wallis test, obtained result that there was a significant difference (p<0.05) epithelialization variable with p value p = 0.000 between control and treatment group. And based on One-way Anova test, obtained result that there was a significant difference (p<0.05) with p value p =0.000 between control and treatment group. The conclusion of this research was giving mahkota dewa fruits (Phaleria macrocarpa) extract can increase epithelialization in incision wound of white rats (Rattus norvegicus). Keywords : Mahkota Dewa Fruits (Phaleria Macrocarpa) Extract, Epithelialization, Incision Wound 

scholarly journals Acceleration of post-tooth extraction socket healing after continuous aerobic and anaerobic physical exercise in Wistar rats (Rattus norvegicus)

2020 ◽  
Vol 53 (4) ◽  
pp. 196
Author(s):  
Aqsa Sjuhada Oki ◽  
Moch Febi Alviansyah ◽  
Christian Khoswanto ◽  
Retno Pudji Rahayu ◽  
Muhammad Luthfi
Keyword(s):  
Wound Healing ◽  
Physical Exercise ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Tooth Extraction ◽  
Anaerobic Exercise ◽  
Control Group ◽  
Continuous Training ◽  
Significant Difference ◽  
Aerobic And Anaerobic

Background: Physical exercise has been proven to accelerate wound healing. Physical training itself consists of aerobic (continuous training) and anaerobic (interval training) exercise. The effectiveness of continuous physical exercise on post-tooth extraction wound healing is the focus of this study. Purpose: This study aims to investigate the differences in post-tooth extraction wound healing in Wistar rats (Rattus norvegicus) after aerobic and anaerobic exercise based on the number of fibroblasts and neovascularisation. Methods: Wistar rats were divided into three groups: the control group (K1); K2 undertook continuous aerobic exercise, swimming at 50% maximum swimming capacity (MSC) with an additional 3% bodyweight load; K3 undertook anaerobic continuous exercise, swimming at 65% MSC with a 6% load. The rats swam three times per week for six weeks. The number of fibroblasts and neovascularisation were examined three days after tooth extraction. Data was analysed using the one-way analysis of variance (ANOVA) and Least Significant Difference (LSD) tests (p<0.05). Results: There was a significant difference in the number of fibroblasts between the K2 and K3 groups. There was no significant difference between K2 and K3 in the amount of neovascularisation. Conclusion: There were differences in the number of fibroblasts but not neovascularisation after tooth extraction in Wistar rats given aerobic and anaerobic continuous training.

scholarly journals Effect of Ethanol Extract of Mangosteen Peel on Serum Malondialdehyde and Leukotriene B4 Levels in Male White Rats (Rattus norvegicus) Wistar Strain Exposed with Electric Cigarette Smoke

2021 ◽  
Vol 56 (4) ◽  
pp. 283
Author(s):  
Jemima Lewi Santoso ◽  
Harianto Notopuro ◽  
Ema Qurnianingsih
Keyword(s):  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Leukotriene B4 ◽  
The Body ◽  
Control Group ◽  
Test Results ◽  
White Rats ◽  
Mangosteen Peel ◽  
Significant Difference

This study aims to analyze the administration of mangosteen peel ethanol extract at different doses can reduce levels of malondialdehyde (MDA) and leukotriene B4 (LTB4) serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke. Electric cigarette induces mitochondrial ROS production and triggers oxidative stress. Antioxidants are needed by the body if there are many free radicals, for example mangosteen peel. This laboratory experimental study uses a randomized post test only control group design. Thirty male white rats were divided into 5 groups randomly (KK, KR, P1, P2, P3). KK given 0.5% Na-CMC, KR given smoke and 0.5% Na-CMC, P1, P2, P3 given smoke and mangosteen peel ethanol extract 100, 200, 300 mg / kg weight. Data were tested for normality and homogeneity tests. Data distribution was found not normal. Data were tested by Kruskal-Wallis Test and Mann-Whitney. Kruskal-Wallis Test results showed significant differences in MDA and LTB4 levels in each experimental group (KS, KR, P1, P2 and P3), p <0.05. Mann-Whitney test results showed a significant difference in the MDA levels of the KR against P3 and in the LTB4 level of the KR against P2 (p <0.05). The conclusion of the study was the administration of mangosteen peel ethanol extract can reduce MDA and LTB4 levels serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke.

scholarly journals Differences Post-extraction Collagen-density of Wistar-rat With Aerobic and Anaerobic Interval-training

2019 ◽  
Vol 2 (2) ◽  
pp. 32
Author(s):  
Aqsa Sjuhada Oki ◽  
Indeswati Diyatri ◽  
Kadek Gede Putra Wibawa
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Tooth Extraction ◽  
Tap Water ◽  
Interval Training ◽  
Wistar Rat ◽  
Control Group ◽  
Significant Difference ◽  
Collagen Density ◽  
Aerobic And Anaerobic

Background: Tooth extraction is extracting teeth from alveolar bone. In the world, tooth loss is a disease that affects the function and aesthetics oral cavity, it causes 7.5 million Disability-Adjusted Life Year (DALY). Tooth extraction rate in Indonesia is around 24% in 2013 and 19% in 2018. Sports is one of the activities that can help accelerate wound healing after tooth extraction, this study examine differences in collagen density after tooth extraction of Wistar rats given aerobic and anaerobic interval training. Objective: To prove differences in post-extraction collagen density of Wistar Rat (Rattus norvegicus) with aerobic and anaerobic interval training. Method: Wistar rats were divided into 3 groups, control group (K) rat were soaked in a bucket with tap water as high as the rat soles with a duration of 50% of maximum swimming ability (KRM), aerobic interval (P1) 3% load swim test with duration of  3 sets 50 % KRM exercise and 25% KRM rest, anaerobic interval P2 load 6% duration 80% KRM exercise and rest with 4 sets. All groups were observed on the 3rd and 7th day post extraction. Data were analyzed using Kruskal-Wallis and continued with Post-hoc test to test differences between groups. Results: There was only significant difference in collagen thickness between groups K(3) and K7, and another groups had not significant difference. Conclusion: there unsignificant differences in collagen density after tooth extraction in Wistar rats (Rattus norvegicus) with aerobic and anaerobic interval training.

scholarly journals POTENSI PEMBERIAN EKSTRAK BUAH DELIMA (Punica granatum L) TERHADAP KUALITAS SPERMATOZOA TIKUS PUTIH (Rattus norvegicus) YANG TERPAPAR PANAS

2020 ◽  
Vol 8 (1) ◽  
pp. 5
Author(s):  
Nucifera Fadhillah Santoso ◽  
Budi Utomo ◽  
Mirni Lamid
Keyword(s):  
Treatment Group ◽  
Rattus Norvegicus ◽  
Membrane Integrity ◽  
Punica Granatum ◽  
Control Treatment ◽  
Control Group ◽  
White Rats ◽  
Significant Difference ◽  
The Difference

The purpose of this research was to determine the effect of pomegranate (Punica granatul l) fruit extract for spermatozoa quality including the motility, viability and membrane integrity of spermatozoa. The researchused 20 male white rats  (Rattus norvegicus) with 200-300 gram average body weight. P0 as control was given with CMC Na 0.5%.  P1 used 75 mg/kg BW dose of extract,  P2used 150 mg/kg BW dose of extract,  P3 used 300 mg/kg BW dose of extract. The treatment was given perorally for 14 days. The experiment was used completely random design (CRD). The result of this study was analyzed with ANOVA followed by Tukey Test (p<0.05). The result showed that there was significant effect from therapy on the quality of rats spermatozoa. The difference of spermatozoa’s concentration between first treatment group P1 and control treatment group is not significant, while the treatment other result showed between the second treatment group and the control group P0. A significant difference occurs in the percentage of motility, viability and  membrane integrity. The treatment from P2 group showed the best result in maintaining the quality of rats spermatozoa.

Test of Burn Wounds Healing Effects of Collagen From Snakehead Fish (Channa striata) Bone in The Preparation of Cream on Male White Rats (Rattus norvegicus)

2020 ◽  
Vol 3 (1) ◽  
pp. 62-75
Author(s):  
Najwa Miladi Hasri ◽  
Nilsya Zebua ◽  
Sudewi
Keyword(s):  
Wound Healing ◽  
Rattus Norvegicus ◽  
Homogeneity Test ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
White Rats ◽  
Significant Difference ◽  
Irritation Test ◽  
Channa Striata ◽  
Post Hoc

Abstract. The use of snakehead fish (Channa striata) collagen in cream is an alternative toreduce fish wastes. It has been proved that snakehead fish (Channa striata) contain collagen.This research aims to study the burn wound healing effectivity of bone’s collagen fromsnakehead fish (Channa striata) that is formulated into a cream. This study used theexperimental method with the bone of snakehead fish (Channa striata) as a sample. This studyconsisted of 4 (four) such as isolation of collagen from snakehead fish's (Channa striata) bone,characterization of collagen by analyzing moisture, ash, protein, fat and functional groupanalysis with FTIR, formulation, and evaluations of cream such as organoleptic test,homogeneity test, pH measurement, stability test, and irritation test, and burn wound healingtest in male white rats (Rattus norvegicus) and the result is analyzed using SPSS 22.0 Free trialand One Way ANOVA and Post-Hoc Tukey HSD. The results showed that fish collagen couldbe isolated from snakehead fish (Channa striata) and the yield obtained is 33.3%. The results ofcollagen characterization and evaluation test met the collagen standard requirements. Results ofthe burn wound healing test on male white rats (Rattus norvegicus) showed that K1 burn woundrecovered on day 21, K2 on day 12, K3 on day 15, K4 on day 18, and K5 on day 18.Measurement of burn wound diameter on day 21 showed K1 = 1.20 cm, K2 = 0.15 cm, K3 =0.10 cm, K4 = 0.45 cm, K5 = 0.40 cm. The results of the statistical analysis of burn wounddiameter showed a significant difference p = 0,000 (p <0.05) between each group. K3 showedthe reduction in the burn wound diameter is faster and the smallest, it can be concluded that theoptimal dose of snakehead fish bone’s collagen cream is a concentration of 3%.

scholarly journals Effect of Ethanol Extract of Mangosteen Peel on Serum Malondialdehyde and Leukotriene B4 Levels in Male White Rats (Rattus norvegicus) Wistar Strain Exposed with Electric Cigarette Smoke

2020 ◽  
Vol 56 (4) ◽  
pp. 283
Author(s):  
Jemima Lewi Santoso ◽  
Harianto Notopuro ◽  
Ema Qurnianingsih
Keyword(s):  
Cigarette Smoke ◽  
Rattus Norvegicus ◽  
Ethanol Extract ◽  
Leukotriene B4 ◽  
The Body ◽  
Control Group ◽  
Test Results ◽  
White Rats ◽  
Mangosteen Peel ◽  
Significant Difference

This study aimed to analyze the administration of mangosteen peel ethanol extract at different doses can reduce levels of malondialdehyde (MDA) and leukotriene B4 (LTB4) serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke. Electric cigarette induces mitochondrial ROS production and triggers oxidative stress. Antioxidants are needed by the body if there are many free radicals, for example mangosteen peel. This laboratory experimental study uses a randomized post-test only control group design. Thirty male white rats were divided into 5 groups randomly (KK, KR, P1, P2, P3). KK given 0.5% Na-CMC, KR given smoke and 0.5% Na-CMC, P1, P2, P3 given smoke and mangosteen peel ethanol extract 100, 200, 300 mg/kg weight. Data were tested for normality and homogeneity tests. Data distribution was found not normal. Data were tested by Kruskal-Wallis Test and Mann-Whitney. Kruskal-Wallis Test results showed significant differences in MDA and LTB4 levels in each experimental group (KS, KR, P1, P2 and P3), p <0.05. Mann-Whitney test results showed a significant difference in the MDA levels of the KR against P3 and in the LTB4 level of the KR against P2 (p <0.05). The conclusion of the study was the administration of mangosteen peel ethanol extract can reduce MDA and LTB4 levels serum in male white rats (Rattus norvegicus) Wistar strains exposed to electric cigarette smoke.

scholarly journals PENGARUH PEMBERIAN EKSTRAK ETANOL DAUN SAUROPUS ANDROGYNUS (L). MERR (KATUK) TERHADAP KADAR HORMON PROLAKTIN PADA TIKUS PUTIH (WISTAR ALBINO MENYUSUI)

2018 ◽  
Vol 12 (3) ◽  
pp. 202
Author(s):  
Sari Ida Miharti ◽  
Fadil Oenzil ◽  
Iskandar Syarif
Keyword(s):  
Hormone Level ◽  
Ethanol Extract ◽  
Control Group ◽  
Control Group Design ◽  
White Rats ◽  
Significant Difference ◽  
Post Test ◽  
Good Nutrition ◽  
Post Hoc ◽  
Prolactin Hormone

<p align="center"><strong>ABSTRACT</strong></p><p align="center"> </p><p>That katuk leaf (Sauropus androgynus (L). Merr) has good nutrition and is able to increase breastmilk production. The study of the effect of katuk leaf ethanol extract administration on prolactin hormone level in the breastfeeding white rats. The purpose of this study was to analyze the effect of katuk leaf ethanol extract administration on prolactin hormone level and oxytocin hormone level in the breastfeeding white rats.</p><p> </p><p>The type of the research is experimental with Post-Test Only Control Group design. The sample size consisted of 24 breastfeeding white rats which are divided into 4 groups, i.e. one control group and three treated groups (P1, P2 and P3), each given 24 mg, 48 mg and 72 mg katuk leaf ethanol extract. The research was conducted at Pharmacy Laboratory and Biomedical Laboratory of University of Andalas, Padang. The prolactin hormone levels are measured by using the ELISA method, the statistical test used One Way ANOVA test and continued with Multifer Comparisons (post hoc test) Bonferroni-type test.</p><p> </p><p>The results showed a significant difference  of the hormone prolactin levels between the control group (13,679 ± 2.061 ng/L) value (p &lt;0.05) and the P3 group (17.509 ± 2.515 ng/L), as well as P1 group (13.816 ± 1.665 ng/L) and P3 group (17.509 ± 2.515 ng/L) in 24 mg and 72 mg of dose.</p><p> </p><p>In conclusion, there was a significant increase in prolactin levels after the administration of katuk leaves extract (Sauropus androgynus (L). Merr) in white rats.</p><p> </p><p> </p><p><strong>Keywords: </strong>Katuk Leaf, Prolaktin,</p><p> </p><p> </p><p align="center"><strong>ABSTRAK</strong></p><p>Daun katuk (<em>Sauropus androgynus</em> (<em>L</em>) <em>Merr</em>) memiliki gizi yang baik dan dapat memperlancar air susu. Penelitian pengaruh pemberian ekstrak etanol daun katuk terhadap hormon prolaktin pada tikus putih. Tujuan Penelitian ini untuk menganalisis pengaruh pemberian ekstrak etanol daun katuk terhadap hormon prolaktin dan hormon oksitosin pada tikus putih meyusui.</p><p><strong>            </strong></p><p>Jenis penelitian ini adalah eksperimental dengan <em>desain Post-Tes Only Control Group</em>. Jumlah sampel 24 tikus menyusui yang dibagi menjadi 4 kelompok, yaitu kelompok kontrol dan 3 kelompok perlakukan P1, P2 dan P3 yang masing–masing diberi 24 mg, 48 mg dan 72 mg ekstrak etanol daun katuk. Penelitian dilakukan di Labor Farmasi dan Biomedik Universitas Andalas. Hormon prolaktin diukur dengan mengunakan metode ELISA uji statistik mengunakan uji <em>One Way </em>ANOVA dan dilanjutkan dengan uji <em>Multiple Comparisons</em> (<em>post hoc test</em>) jenis <em>Bonferroni</em>.</p><p> </p><p>Hasil penelitian ini menunjukakan terdapat perbedaan kadar hormon prolaktin antara kelompok kontrol (13,679±2,061ng/L) nilai (p&lt;0,05) dengan kelompok P3 (17,509±2,515 ng/L) dan kelompok P1(13,816±1,665 ng/L) dengan kelompok P3 (17,509±2,515 ng/L) dengan dosis 24 mg dan 72 mg.</p><p>            Kesimpulan, terdapat peningkatan signifikan pada kadar hormon prolaktin setelah pemberian ekstrak etanol daun katuk (<em>Sauropus androgynus</em> (<em>L</em>) <em>Merr</em>) pada tikus putih menyusui</p><p> </p><p> </p><p> </p><p> </p><p><strong>Kata kunci     : </strong>Daun Katuk, Prolaktin,</p>

Clinical Impact of Co-medication of Levetiracetam and Clobazam with Proton Pump Inhibitors: A Drug Interaction Study

2020 ◽  
Vol 21 (2) ◽  
pp. 126-131
Author(s):  
Bhuvanachandra Pasupuleti ◽  
Vamshikrishna Gone ◽  
Ravali Baddam ◽  
Raj Kumar Venisetty ◽  
Om Prakash Prasad
Keyword(s):  
Plasma Concentration ◽  
Proton Pump Inhibitors ◽  
Proton Pump ◽  
Plasma Concentrations ◽  
Control Group ◽  
Drug Concentrations ◽  
Significant Difference ◽  
Post Hoc ◽  
Hydrolysis Of ◽  
Cytochrome P 450

Background: Clobazam (CLBZ) metabolized primarily by Cytochrome P-450 isoenzyme CYP3A4 than with CYP2C19, Whereas Levetiracetam (LEV) is metabolized by hydrolysis of the acetamide group. Few CYP enzymes are inhibited by Proton Pump Inhibitors (PPIs) Pantoprazole, Esomeprazole, and Rabeprazole in different extents that could affect drug concentrations in blood. The aim of the present study was to evaluate the effect of these PPIs on the plasma concentrations of LEV and CLBZ. Methods: Blood samples from 542 patients were included out of which 343 were male and 199 were female patients and were categorized as control and test. Plasma samples analyzed using an HPLC-UV method. Plasma concentrations were measured and compared to those treated and those not treated with PPIs. One way ANOVA and games Howell post hoc test used by SPSS 20 software. Results: CLBZ concentrations were significantly 10 folds higher in patients treated with Pantoprazole (P=0.000) and 07 folds higher in patients treated with Esmoprazole and Rabeprazole (P=0.00). Whereas plasma concentration of LEV control group has no statistical and significant difference when compared to pantoprazole (P=0.546) and with rabeprazole and esomeprazole was P=0.999. Conclusion: The effect of comedication with PPIs on the plasma concentration of clobazam is more pronounced for pantoprazole to a greater extent when compared to esomeprazole and rabeprazole. When pantoprazole is used in combination with clobazam, dose reduction of clobazam should be considered, or significance of PPIs is seen to avoid adverse effects.

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