scholarly journals BETEL LEAF EXTRACTS (PIPER BETLE L.) PREVENT ENDOTHELIAL DYSFUNCTION BY REDUCING THE LEVELS OF URIC ACID AND ICAM-1 EXPRESSION OF THE HYPERURICEMIA WISTAR RATS (RATTUS NORVEGICUS) AORTIC ENDOTHELIAL

2016 ◽  
Vol 10 (1) ◽  
pp. 1
Author(s):  
Made Sumarya ◽  
Nyoman Adiputra ◽  
Putra Manuaba ◽  
Dewa Sukrama
Keyword(s):  
Uric Acid ◽  
Endothelial Dysfunction ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Piper Betle ◽  
Leaf Extracts ◽  
Betel Leaf ◽  
Aortic Endothelial

scholarly journals Pemberian ekstrak daun sirih (Piper betle) secara topikal meningkatkan ketebalan epidermis, jumlah fibroblas, dan jumlah kolagen dalam proses penyembuhan luka pada tikus jantan galur Wistar (Rattus norvegicus)

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Eva F. Palumpun ◽  
Anak A.G.P. Wiraguna ◽  
Wimpie Pangkahila
Keyword(s):  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Healing Process ◽  
Wound Healing Process ◽  
Piper Betle ◽  
Epidermal Thickness ◽  
Betel Leaf ◽  
Oral Amoxicillin ◽  
Male Wistar Rats ◽  
Sirius Red

Abstract: This study was aimed to prove that topical betel (Piper betle) leaf extract adinistration could increase epidermal thickness, fibroblasts, and collagen amount in wound healing process of male Wistar rats (Rattus norvegicus). Subjects were 36 Wistar rats (Rattus norvegicus) with inclusion criteria, as follows: healthy, aged 3-4 months, weighing 200-250 g, divided into two groups with 18 rats each. The first group, the control group (P0), was treated with oral amoxicillin 3x10mg/day for 3 days and one drop (50 μl) of 10% povidine iodine topically 2x/day for 14 days, and the second group, the treatment group (P1), treated with oral amoxicillin 3x10 mg/day for 3 days and one drop (50 μl) of 10% betel leaf (Piper betle) extracttopically 2x/day for 14 days. Samples of skin tissue were processed for histological slides by using hematoxylin-eosin staining to check the epidermal thickness and fibroblast, meanwhile Picro sirius red staining to check the collagen amount. Microscopic examinations showed that the average epidermal thickness in P0 group was 24.72±14.91 μm, whereas in the P1 group was 56.75±23.04 μm (P <0.01). The number of fibroblasts in P0 group was 75,45±32,52 cells/visual field meanwhile of P1 group was 95,67±22,51 cells/visual field (P < 0.05). The average of collagen amount in P0 group was 65.27±7.13% while in P1 group was 83.09±2.59% (P <0.01). Conclusion: Topical administration of 10% betel (Piper betle) leaf extract could increase epidermal thickness, fibroblasts, and collagen in wound healing process of male Wistar rats (Rattus norvegicus).Keywords: betel leaf, epidermis, fibroblast, collagen, wound Abstrak: Tujuan penelitian ini ialah untuk membuktikan bahwa pemberian ekstrak daun sirih (Piper betle) meningkatkan ketebalan epidermis, jumlah fibroblas, dan jumlah kolagen dalam proses penyembuhan luka pada tikus jantan galur Wistar (Rattus norvegicus). Subjek penelitian ialah 36 ekor tikus jantan galur Wistar (Rattus norvegicus) dewasa dan sehat, berumur 3-4 bulan, dengan berat badan 200-250 gr, yang terbagi menjadi 2 (dua) kelompok masing-masing berjumlah 18 ekor tikus. Kelompok pertama ialah kelompok kontrol (P0) diberikan amoksisilin oral 3 x 10mg/hari selama 3 hari serta povidine iodine 10% topikal 1 tetes (50 μl) 2x/hari selama 14 hari (P0). Kelompok kedua ialah kelompok perlakuan (P1) diberi amoksisilin oral 3 x 10 mg/hari selama 3 hari serta ekstrak daun sirih (Piper betle) konsentasi 10% secara topikal 1 tetes (50 μl), 2x/hari selama 14 hari. Jaringan kulit diambil dan dibuat preparat dengan pewarnaan hematoksilin-eosin untuk pemeriksaan ketebalan epidermis dan jumlah fibroblas, serta pewarnaan Picro sirius red untuk pemeriksaan jumlah kolagen. Hasil pemeriksaan mikroskopik menunjukkan rerata tebal epidermis pada kelompok P0 24,72±14,91 μm dan pada kelompok P1 56,75±23,04 μm (P <0,01). Rerata jumlah fibroblas pada kelompok P0 75,45±32,52 sel/lapang pandang dan pada kelompok P1 95,67±22,51 sel/lapang pandang (P <0,05). Rerata jumlah kolagen pada kelompok P0 65,27±7,13% dan pada kelompok P1, 83,09±2,59% (P <0,01). Simpulan: Pemberian ekstrak daun sirih (Piper betle) konsentrasi 10% secara topikal dapat meningkatkan ketebalan epidermis, jumlah fibroblas, dan jumlah kolagen pada luka tikus jantan galur Wistar (Rattus norvegicus). Kata kunci: daun sirih, epdermis, fibroblas, kolagen, luka

scholarly journals Effect of Piper betle Leaf Extract-Loaded Chitosan Nano Particle as Fruit-Coating Assay to Control Anthracnose on Mango

2020 ◽  
Vol 11 (1) ◽  
pp. 48-62
Author(s):  
Yadi - Suryadi ◽  
I Made - Samudra ◽  
Mitha Eka - Puteri ◽  
Tetty - Kemala
Keyword(s):  
Phenolic Compounds ◽  
Leaf Extract ◽  
Sodium Tripolyphosphate ◽  
Chitosan Nanoparticles ◽  
Piper Betle ◽  
Leaf Extracts ◽  
Betel Leaf ◽  
Chitosan Nanoparticle ◽  
Wave Numbers ◽  
Chitosan Hydrolysis

Green betel leaf (Piper betle L.) is one of the plants being used for traditional herbal medicine. This study aimed to determine betel leaf extract-chitosan nanoparticles to control anthracnose disease on mango. Chitosan nanoparticles were prepared by ionic gelation method using sodium tripolyphosphate as cross-linking agent. Characterization of the betel leaf extract was done by pyrolysis GC-MS; while chitosan nanoparticles were characterized using FTIR spectroscopy, SEM, and PSA analysis. The results showed that the green betel leaf extract contains 71.18 + 0.3% of antifungal phenolic compounds. The most phenolic compounds and their derivatives in the betel leaf extract was 1-hydroxy-4-methylbenzotriazole. Chitosan hydrolysis reduced the chitosan MW from 754.89 kDa to 245.85 kDa. Based on FTIR analysis, hydrolysis treatment and the addition of extract affected the existence of chitosan functional group, and wave numbers. The absorption of aromatic groups was observed at 1000 - 650 cm-1 wave numbers. The sizes of particle were ranged from 101 + 6.25 nm to 431.1 + 4.32 nm. The size of chitosan without hydrolysis was bigger than that of chitosan with hydrolysis. The SEM morphology of the chitosan nanoparticle-betel leaf extract was spherical shape. Chitosan hydrolysis treatment had a higher antifungal effect than that of chitosan without hydrolysis. The mass ratio of chitosan nanoparticles and betel leaf extracts (3:1; v/v) of both without hydrolysis and with hydrolysis was found as a good formula in suppressing anthracnose on mangoes with the degree of disease inhibition of 85.88% and 98.82%, respectively. The betel leaf extract-loaded chitosan nanoparticle treatment may offer the fruit shelf life up to 6 days.

scholarly journals Kadar Kolesterol, Asam Urat dan Glukosa Darah Ayam Petelur yang Diberi Jus Daun Sirih (Piper betle Linn) pada Level yang Berbeda

2021 ◽  
Vol 11 (1) ◽  
pp. 76
Author(s):  
Rusli Badaruddin ◽  
Rahim Aka ◽  
Abdul Rahman Ollong ◽  
Ning Ayu Dwi Tiya
Keyword(s):  
Uric Acid ◽  
Blood Glucose ◽  
Piper Betle ◽  
Betel Leaf ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Commercial Feed ◽  
Individual Cage ◽  
Range Test ◽  
Leaf Juice

ABSTRACT          This study aims to assess cholesterol, uric acid and blood glucose levels in laying hens which are fed with the addition of betel leaf juice (Piper Bettle Linn). There were 16 chickens used in layer phase hens. The cage used is an individual cage equipped with a place for feeding and drinking water. The treatments in this study consisted of: (P0) commercial feed + 0 ml of betel leaf juice, (P1) commercial feed + 5 ml of betel leaf juice, (P2) commercial feed + 10 ml of betel leaf juice, and (P3) commercial feed + 15 ml of betel leaf juice. The variables observed were cholesterol, uric acid and blood glucose levels. The data obtained from the research results were analyzed using variance and continued with Duncan's multiple range test. The results showed that the administration of betel leaf juice had no significant effect (p <0.05) on cholesterol, uric acid and blood glucose levels in layer phase hens. Based on the results of the study, it can be concluded that the administration of betel leaf juice does not affect the amount of cholesterol, uric acid, and glucose levels in layer phase hens, but these conditions are still in normal physiological conditions. Keywords: uric acid, layer hens, glucose, cholesterol ABSTRAK Penelitian ini bertujuan untuk mengkaji tentang kadar kolesterol, asam urat dan glukosa darah ayam petelur yang diberi penambahan jus daun sirih (Piper betle Linn) pada level yang berbeda. Ayam yang digunakan sebanyak 16 ekor induk ayam petelur betina fase layer. Kandang yang digunakan yaitu kandang individu yang dilengkapi dengan tempat pakan dan air minum. Perlakuan pada penelitian ini terdiri atas: (P0) pakan basal + 0 ml jus daun sirih,  (P1) pakan basal + 5 ml jus daun sirih,  (P2) pakan basal + 10 ml jus daun sirih, dan (P3) pakan basal + 15 ml jus daun sirih. Variabel yang diamati adalah kadar kolesterol, asam urat, dan glukosa darah. Data yang diperoleh dari hasil penelitian dianalisis menggunakan sidik ragam dan dilanjutkan dengan uji jarak berganda Duncan. Hasil penelitian menunjukkan bahwa pemberian jus daun sirih tidak memberikan pengaruh yang nyata (p<0.05) terhdadap kadar kolesterol, asam urat dan glukosa darah ayam petelur fase layer. Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian jus daun sirih tidak mempengaruhi jumlah kadar kolestero, asam urat, dan glukosa ayam petelur fase layer, namun kondisi tersebut masih dalam kondisi fisiologis normal. Kata Kunci : asam urat, ayam petelur, glukosa, kolesterol  

scholarly journals STANDARISASI EKSTRAK DAUN SIRIH HIJAU (Piper betle L.) DAN UJI ANTIBAKTERI TERHADAP BAKTERI Pseudomonas aeruginosa

PHARMACON ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 533
Author(s):  
Geraldin Ester Manarisip ◽  
Fatimawali Fatimawa;i ◽  
Henki Rotinsulu
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Specific Gravity ◽  
Moisture Content ◽  
Optical Density ◽  
Drying Shrinkage ◽  
Water Soluble ◽  
Piper Betle ◽  
Leaf Extracts ◽  
Betel Leaf ◽  
Dark Green

ABSTRACTThis study aims to determine the standardization of betel leaf extracts and determine the concentration of extracts that can inhibit bacteria. In this study, the extraction was carried out by the maceration method. The specific parameters were identity of the extracts, organoleptic test, levels of water, ethanol soluble compounds, and chemical content, meanwhile, the non-specific parameters were drying loss, moisture content, and specific gravity. The results of the standardization of specific parameters showed the name of the Green Betel leaf extract (Piper betle L.), organoleptic (thick, dark green color, and strong betel odor), water-soluble and ethanol-soluble extracts content (68.27% and 82%), containing alkaloid compounds, steroids, and tannins. The results of non-specific parameters showed drying shrinkage (10.91%), moisture content (22.73%), and specific gravity (0.874 g / mL). Antibacterial test against Pseudomonas aeruginosa showed that betel leaf extracts at a concentration of 25%, 20%, and 15% experienced a decrease in Optical Density (OD) values respectively -0.192, -0.065, -0.098 while at concentrations of 10% and 5% had an increase Optical Density (OD) values were 0.512, 0.767, respectively. From the results obtained, it was concluded that the betel leaf extracts (Piper betle L.) fulfilled specific and non-specific parameters and had the ability to inhibit the growth of Pseudomonas aeruginosa bacteria with an MIC value of 15%.Keywords: Betel leaf (Piper betle L.), Standardization, Antibacterial, Pseudomonas aeruginosa.  ABSTRAKPenelitian ini bertujuan untuk menentukan standarisasi ekstrak daun sirih hijau dan menentukan konsentrasi ekstrak yang mampu menghambat bakteri. Dalam penelitian ini penyarian dilakukan dengan metode maserasi. Parameter spesifik yaitu identitas ekstrak, uji organoleptik, kadar senyawa larut air dan larut etanol, dan kandungan kimia, sedangkan parameter non spesifik yaitu susut pengeringan, kadar air, dan bobot jenis. Hasil standarisasi parameter spesifik menunjukkan nama ekstrak etanol Daun Sirih Hijau (Piper betle L.), organoleptik (kental, warna hijau pekat, dan bau khas sirih yang tajam), kadar sari larut air dan larut etanol masing masing (68,27% dan 82%), dengan kandungan senyawa alkaloid, steroid, tanin. Hasil parameter non spesifik menunjukkan nilai susut pengeringan (10,91%), kadar air (22,73%), dan bobot jenis (0,874 g/mL). Uji antibakteri terhadap Pseudomonas aeruginosa menunjukkan bahwa ekstrak daun sirih pada konsentrasi 25%, 20%, dan 15% mengalami penurunan nilai Optical Density (OD) berturut-turut -0,192, -0,065, -0,098 sedangkan pada konsentrasi 10% dan 5% mengalami kenaikan nilai Optical Density (OD) berturut-turut 0,512, 0,767. Dari hasil yang diperoleh disimpulkan bahwa ekstrak daun sirih (Piper betle L.) memenuhi parameter spesifik dan non spesifik dan memiliki kemampuan menghambat pertumbuhan bakteri Pseudomonas aeruginosa dengan nilai KHM 15%.Kata kunci: Daun Sirih (Piper betle L.), Standarisasi, Antibakteri, Pseudomonas aeruginosa.

scholarly journals Preliminary study on the potential of topical anaesthesia from betel leaf and clove leaf extract

2021 ◽  
Vol 33 (3) ◽  
pp. 250
Author(s):  
Ahmad Ronal ◽  
Fadhilah Nadya Astuti ◽  
Lintang Pratiwi ◽  
Lisa Prihastari
Keyword(s):  
Wistar Rats ◽  
Leaf Extract ◽  
Piper Betle ◽  
Syzygium Aromaticum ◽  
Topical Anaesthesia ◽  
Depth Of Anesthesia ◽  
Topical Anesthetic ◽  
Duration Of Action ◽  
Betel Leaf ◽  
Anesthetic Agents

Introduction: Betel leaf (Piper betle Linn.) and clove leaf (Syzygium aromaticum) are Indonesian herbs which are known to cause a numb sensation. The main ingredients of betel leaf, which act as an anesthetic, are caryophyllene, eugenol, methyl eugenol, eucalyptol (1.8-cineol), linalool, α-Pinene, estragole, while cloves have local anesthetic effects through their ingredients, namely eugenol, and β-Caryophyllene. Research on the benefits of these two ingredients as topical anesthetics in Indonesia is still rare. This study was aimed to describe the potential of betel leaf and clove leaf extract as a topical anesthetic in Wistar rats. Methods: This research was a laboratory experimental research with simple randomized controlled design. The sample consisted of 6 male Wistar rats, each of which was given four treatments, namely extract with a concentration of 50%, 75%, and 100%, and topical anaesthetic benzocaine 20% as a control. Each treatment was tested using an electric stimulator and observed onset, duration of action, and depth of topical anaesthesia. The data were processed using descriptive statistics with tables and graphs. Results: Betel leaf extract with a concentration of 100% had a better mean onset, duration of work, and depth of anesthesia than the 20% benzocaine control, whereas 75% clove leaf extract had the longest duration of action compared to 20% benzocaine and 100% concentration was the topical anesthetic agent with the highest anesthetic depth. Conclusion: Based on the onset, duration of action, and depth of anesthesia, betel leaf, and clove leaf have potential as topical anesthetic agents.

scholarly journals Uji efek ekstrak daun sirih hutan (Piper aduncum L.) terhadap kadar gula darah pada tikus wistar (Rattus novergicus) yang diinduksi aloksan

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Silvia R.H. Sitinjak ◽  
Jane Wuisan ◽  
Christi Mambo
Keyword(s):  
Blood Glucose ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Control Group ◽  
Betel Leaf ◽  
Glucose Levels ◽  
Blood Glucose Levels ◽  
Decrease Blood Glucose ◽  
Piper Aduncum

Abstract: Forest betel leaf (Piper aduncum L.) has been known bypublicto have efficacy in wound healing, stop vomiting, reducing nausea, aiding digestion, as an antiseptic, as well as killing bacteria, fungi and viruses. Dewi et al. found that 50 mg/kgBW and 100 mg/kgBW red betel leaf extract caused a decrease blood glucose levels in Wistar rats (Rattus norvegicus) induced by alloxan. This study was aimed to identify the effect of forest betel leaf extract on blood glucose levels on Wistar rats induced by alloxan. Subjects were Wistar rats with total of 15 samples divided into five groups: a negative control group and four hyperglycemic groups induced by 130 mg/kgBW of alloxan. Forest betel leaf extract was administered with dose of 25 mg/kgBW, 50 mg/kgBW, and 100 mg/kgBW on each groups of hyperglycemic rats, and group0.4 IU/100grBW of novomix injection was administered on the positive control group. Blood glucose levels were measured in the 0, 30, 60, 90, and 120 minutes on day one, two, and three. The results showed that 25 mg/kgBW, 50 mg/kgBW, and 100 mg/kgBW of forest betel leaf extract had a tendency to decrease blood glucose levels of Wistar rats induced by alloxan.Keywords: piper aduncum L., forest betel leaf, blood glucose levels, alloxan Abstrak: Daun sirih hutan (Piper aduncum L.) telah dikenal oleh masyarakat dan mempunyai khasiat dalam penyembuhan luka, menghentikan muntah, mengurangi mual, melancarkan pencernaan, sebagai antiseptik, membunuh bakteri dan jamur serta virus. Menurut penelitian Dewi dkk, pemberian ekstrak daun sirih merah dengan dosis 50 mg/kgBB dan 100 mg/kgBB menyebabkan penurunan kadar gula darah pada tikus Wistar (Rattus norvegicus) yang diinduksi dengan aloksan. Penelitian ini bertujuan untuk mengetahui efek ekstrak daun sirih hutan terhadap kadar gula darah pada tikus Wistar yang diinduksi dengan aloksan. Subjek penelitian berupa tikus Wistar berjumlah 15 ekor yang dibagi dalam 5 kelompok terdiri atas kelompok kontrol negatif dan 4 kelompok tikus Wistar yang diberi aloksan dengan dosis 130 mg/kgBB tikus yang menyebabkan tikus Wistar dalam keadaan hiperglikemik. Tikus hiperglikemik diberi ekstrak daun sirih hutan dengan dosis 25 mg/kgBB tikus, 50 mg/kgBB tikus, dan 100 mg/kgBB tikus, dan yang terakhir kelompok kontrol positif diberi suntikan novomix 0,4 IU/100grBB tikus. Data diperoleh dari pemeriksaan kadar gula darah pada semua kelompok pada hari pertama, kedua, dan ketiga pada menit ke-0, 30, 60, 90, dan 120. Hasil penelitian menunjukkan bahwa pemberian ekstrak daun sirih hutan dengan dosis 25 mg/kgBB tikus, 50 mg/kgBB dan 100 mg/kgBB tikus memiliki kecenderungan untuk menurunkan kadar gula darah tikus Wistar yang diinduksi oleh aloksan. Kata kunci: piper aduncumL., daun sirih hutan, kadar gula darah, aloksan

Anti-plaque activity of Piper betle leaf extracts

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
N Pandita ◽  
A Varghese ◽  
M Mantri ◽  
Y Kachwala
Keyword(s):  
Piper Betle ◽  
Leaf Extracts

Efektifitas Ekstrak Daun Sirih Hijau (Piper Betle) sebagai Insektisida Nabati terhadap Mortalitas Lalat Rumah (Musca domestica)

2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF
Keyword(s):  
Musca Domestica ◽  
Leaf Extract ◽  
Effective Concentration ◽  
P Value ◽  
Piper Betle ◽  
Microbiology Laboratory ◽  
House Flies ◽  
Betel Leaf ◽  
The One ◽  
One Way Anova

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies

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