Variation of the Rate of Extension of Actin Networks

1996 ◽  
Vol 463 ◽  
Author(s):  
Donald J. Olbris ◽  
Judith Herzfeld
Keyword(s):  
Fluid Flow ◽  
Cell Membrane ◽  
Actin Filaments ◽  
Sea Cucumber ◽  
Diffusive Flux ◽  
Bulk Fluid ◽  
Actin Networks ◽  
Self Assembling ◽  
Wide Range ◽  
Diffusive Movement

ABSTRACTSelf-assembling protein filaments are important components of a cell's superstructure. Among these, actin filaments form the backbone of protrusions and extensions such as pseudopodia. The rates at which these structures extend cover a startlingly wide range: the acrosomal process of the sea cucumber may extend 90 μm in 10 seconds, which is more than 20 times the speed at which an epithelial goldfish keratocyte crawls. We seek to explain this range by examining the delivery of actin monomers to the growing filament ends. We show that the diffusive flux of actin monomers is adequate for fueling the slower movement of crawling cells, but is insufficient to propel the quicker acrosomal process of the sea cucumber. By introducing bulk fluid flow in response to the diffusive movement of water through the cell membrane, actin delivery can be enhanced. We compare the calculated speeds to experimental observations and discuss future refinements to the model.

Factors Affecting Molecular Self-Assembly and Its Mechanism

2012 ◽  
Vol 9 (1) ◽  
pp. 43 ◽  
Author(s):  
Hueyling Tan
Keyword(s):  
Self Assembly ◽  
Building Blocks ◽  
Molecular Engineering ◽  
Molecular Structures ◽  
Polymer Science ◽  
New Approach ◽  
Factors Affecting ◽  
Dna Structures ◽  
Self Assembling ◽  
Wide Range

Molecular self-assembly is ubiquitous in nature and has emerged as a new approach to produce new materials in chemistry, engineering, nanotechnology, polymer science and materials. Molecular self-assembly has been attracting increasing interest from the scientific community in recent years due to its importance in understanding biology and a variety of diseases at the molecular level. In the last few years, considerable advances have been made in the use ofpeptides as building blocks to produce biological materials for wide range of applications, including fabricating novel supra-molecular structures and scaffolding for tissue repair. The study ofbiological self-assembly systems represents a significant advancement in molecular engineering and is a rapidly growing scientific and engineering field that crosses the boundaries ofexisting disciplines. Many self-assembling systems are rangefrom bi- andtri-block copolymers to DNA structures as well as simple and complex proteins andpeptides. The ultimate goal is to harness molecular self-assembly such that design andcontrol ofbottom-up processes is achieved thereby enabling exploitation of structures developed at the meso- and macro-scopic scale for the purposes oflife and non-life science applications. Such aspirations can be achievedthrough understanding thefundamental principles behind the selforganisation and self-synthesis processes exhibited by biological systems.

scholarly journals Self-assembling ferritin-dendrimer nanoparticles for targeted delivery of nucleic acids to myeloid leukemia cells

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Federica Palombarini ◽  
Silvia Masciarelli ◽  
Alessio Incocciati ◽  
Francesca Liccardo ◽  
Elisa Di Fabio ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Targeted Delivery ◽  
Myeloid Leukemia ◽  
Electrostatic Interactions ◽  
Archaeoglobus Fulgidus ◽  
Leukemia Cells ◽  
Cellular Delivery ◽  
Self Assembling ◽  
Wide Range ◽  
Hybrid Nanoparticle

Abstract Background In recent years, the use of ferritins as nano-vehicles for drug delivery is taking center stage. Compared to other similar nanocarriers, Archaeoglobus fulgidus ferritin is particularly interesting due to its unique ability to assemble-disassemble under very mild conditions. Recently this ferritin was engineered to get a chimeric protein targeted to human CD71 receptor, typically overexpressed in cancer cells. Results Archaeoglobus fulgidus chimeric ferritin was used to generate a self-assembling hybrid nanoparticle hosting an aminic dendrimer together with a small nucleic acid. The positively charged dendrimer can indeed establish electrostatic interactions with the chimeric ferritin internal surface, allowing the formation of a protein-dendrimer binary system. The 4 large triangular openings on the ferritin shell represent a gate for negatively charged small RNAs, which access the internal cavity attracted by the dense positive charge of the dendrimer. This ternary protein-dendrimer-RNA system is efficiently uptaken by acute myeloid leukemia cells, typically difficult to transfect. As a proof of concept, we used a microRNA whose cellular delivery and induced phenotypic effects can be easily detected. In this article we have demonstrated that this hybrid nanoparticle successfully delivers a pre-miRNA to leukemia cells. Once delivered, the nucleic acid is released into the cytosol and processed to mature miRNA, thus eliciting phenotypic effects and morphological changes similar to the initial stages of granulocyte differentiation. Conclusion The results here presented pave the way for the design of a new family of protein-based transfecting agents that can specifically target a wide range of diseased cells. Graphic abstract

Capillary Equilibrium in Porous Materials

1965 ◽  
Vol 5 (01) ◽  
pp. 15-24 ◽  
Author(s):  
Norman R. Morrow ◽  
Colin C. Harris
Keyword(s):  
Mass Transfer ◽  
Fluid Flow ◽  
Porous Materials ◽  
Capillary Pressure ◽  
Chemical Engineering ◽  
Fluid Distribution ◽  
Bulk Fluid ◽  
Fluid Saturation ◽  
Capillary Pressure Curves ◽  
The Relationship

Abstract The experimental points which describe capillary pressure curves are determined at apparent equilibria which are observed after hydrodynamic flow has ceased. For most systems, the time required to obtain equalization of pressure throughout the discontinuous part of a phase is prohibitive. To permit experimental points to be described as equilibria, a model of capillary behavior is proposed where mass transfer is restricted to bulk fluid flow. Model capillary pressure curves follow if the path described by such points is independent of the rate at which the saturation was changed to attain a capillary pressure point. A modified suction potential technique is used to study cyclic relationships between capillary pressure and moisture content for a porous mass. The time taken to complete an experiment was greatly reduced by using small samples. Introduction Capillary retention of liquid by porous materials has been investigated in the fields of hydrology, soil science, oil reservoir engineering, chemical engineering, soil mechanics, textiles, paper making and building materials. In studies of the immiscible displacement of one fluid by another within a porous bed, drainage columns and suction potential techniques have been used to obtain relationships between pressure deficiency and saturation (Fig. 1). Except where there is no hysteresis of contact angle and the solid is of simple geometry, such as a tube of uniform cross section, there is hysteresis in the relationship between capillary pressure and saturation. The relationship which has received most attention is displacement of fluid from an initially saturated bed (Fig. 1, Curve Ro), the final condition being an irreducible minimum fluid saturation Swr. Imbibition (Fig. 1, Curve A), further desaturation (Fig. 1, Curve R), and intermediate scanning curves have been studied to a lesser but increasing extent. This paper first considers the nature of the experimental points tracing the capillary pressure curves with respect to the modes and rates of mass transfer which are operative during the course of measurement. There are clear indications that the experimental points which describe these curves are obtained at apparent equilibria which are observed when viscous fluid flow has ceased; and any further changes in the fluid distribution are the result of much slower mass transfer processes, such as diffusion. Unless stated otherwise, this discussion applies to a stable packing of equal, smooth, hydrophilic spheres supported by a suction plate with water as the wetting phase and air as the nonwetting phase. SPEJ P. 15ˆ

Internal heat generation/or absorption phenomena in three-dimensional natural convection flow within enclosure filled with different working fluids

2018 ◽  
Vol 28 (4) ◽  
pp. 878-908
Author(s):  
Najib Hdhiri ◽  
Brahim Ben Beya
Keyword(s):  
Fluid Flow ◽  
Heat Generation ◽  
Average Nusselt Number ◽  
Thermal Field ◽  
Three Dimensional ◽  
Grashof Number ◽  
Content Type ◽  
Wide Range ◽  
Heat Generation Or Absorption ◽  
2D Model

Purpose The purpose of this study is to investigate the effects of heat generation or absorption on heat transfer and fluid flow within two- and three-dimensional enclosure for homogeneous medium filled with different metal liquid. Numerical results are presented and analyzed in terms of fluid flow, thermal field structures, as well as average Nusselt number profiles over a wide range of dimensionless quantities, Grashof number (Gr) (104 and 105), SQ (varied between −500 to 500) and Prandtl number (Pr = 0.015, 0.024 and 0.0321). The results indicate that when the conductive regime is established for a Grashof number Gr = 104, the 2D model is valid and predicts all three-dimensional results with negligible difference. This was not the case in the convective regime (Gr = 105) where the effect of the third direction becomes important, where a 2D-3D difference was seen with about 37 per cent. Also, in most cases, the authors find that the heat absorption phenomena have the opposite effect with respect to the heat generation. Design/methodology/approach Numerical results are presented and analyzed in terms of fluid flow, thermal field structures, as well as average Nusselt number profiles over a wide range of dimensionless quantities. Findings Grashof number (Gr) (104 and 105), SQ (varied between −500 to 500) and Prandtl number (Pr = 0.015, 0.024 and 0.0321). Originality/value The results indicate that when the conductive regime is established for a Grashof number Gr = 104, the 2D model is valid and predicts all three-dimensional results with negligible difference.

scholarly journals De novo design of self-assembling helical protein filaments

Science ◽  
2018 ◽  
Vol 362 (6415) ◽  
pp. 705-709 ◽  
Author(s):  
Hao Shen ◽  
Jorge A. Fallas ◽  
Eric Lynch ◽  
William Sheffler ◽  
Bradley Parry ◽  
...  
Keyword(s):  
De Novo ◽  
Computational Design ◽  
Building Blocks ◽  
Repeat Units ◽  
Self Assembling ◽  
Wide Range ◽  
Helical Protein ◽  
Monomeric Proteins

We describe a general computational approach to designing self-assembling helical filaments from monomeric proteins and use this approach to design proteins that assemble into micrometer-scale filaments with a wide range of geometries in vivo and in vitro. Cryo–electron microscopy structures of six designs are close to the computational design models. The filament building blocks are idealized repeat proteins, and thus the diameter of the filaments can be systematically tuned by varying the number of repeat units. The assembly and disassembly of the filaments can be controlled by engineered anchor and capping units built from monomers lacking one of the interaction surfaces. The ability to generate dynamic, highly ordered structures that span micrometers from protein monomers opens up possibilities for the fabrication of new multiscale metamaterials.

scholarly journals Insights into the Mode of Action of Chitosan as an Antibacterial Compound

2008 ◽  
Vol 74 (12) ◽  
pp. 3764-3773 ◽  
Author(s):  
Dina Raafat ◽  
Kristine von Bargen ◽  
Albert Haas ◽  
Hans-Georg Sahl
Keyword(s):  
Antimicrobial Activity ◽  
Cell Wall ◽  
Cell Membrane ◽  
Mode Of Action ◽  
Membrane Lipids ◽  
Expression Profiles ◽  
Transcriptional Response ◽  
Response Analysis ◽  
Sequence Of Events ◽  
Wide Range

ABSTRACT Chitosan is a polysaccharide biopolymer that combines a unique set of versatile physicochemical and biological characteristics which allow for a wide range of applications. Although its antimicrobial activity is well documented, its mode of action has hitherto remained only vaguely defined. In this work we investigated the antimicrobial mode of action of chitosan using a combination of approaches, including in vitro assays, killing kinetics, cellular leakage measurements, membrane potential estimations, and electron microscopy, in addition to transcriptional response analysis. Chitosan, whose antimicrobial activity was influenced by several factors, exhibited a dose-dependent growth-inhibitory effect. A simultaneous permeabilization of the cell membrane to small cellular components, coupled to a significant membrane depolarization, was detected. A concomitant interference with cell wall biosynthesis was not observed. Chitosan treatment of Staphylococcus simulans 22 cells did not give rise to cell wall lysis; the cell membrane also remained intact. Analysis of transcriptional response data revealed that chitosan treatment leads to multiple changes in the expression profiles of Staphylococcus aureus SG511 genes involved in the regulation of stress and autolysis, as well as genes associated with energy metabolism. Finally, a possible mechanism for chitosan's activity is postulated. Although we contend that there might not be a single classical target that would explain chitosan's antimicrobial action, we speculate that binding of chitosan to teichoic acids, coupled with a potential extraction of membrane lipids (predominantly lipoteichoic acid) results in a sequence of events, ultimately leading to bacterial death.

Potential role of a series of lysine-/leucine-rich antimicrobial peptide in inhibiting lipopolysaccharide-induced inflammation

2018 ◽  
Vol 475 (22) ◽  
pp. 3687-3706 ◽  
Author(s):  
Weibing Dong ◽  
Xin Zhu ◽  
Xuan Zhou ◽  
Ying Yang ◽  
Xin Yan ◽  
...  
Keyword(s):  
Cell Membrane ◽  
Signaling Pathway ◽  
Inflammatory Responses ◽  
Antimicrobial Activities ◽  
Host Cells ◽  
Inflammatory Factors ◽  
Human Macrophage ◽  
U937 Cells ◽  
Wide Range ◽  
Anti Inflammatory

Antimicrobial peptides have broad-spectrum killing activities against bacteria, enveloped viruses, fungi and several parasites via cell membrane permeation and exhibit primarily immunomodulatory and anti-infective functions in their interactions with host cells. However, the mechanism underlying their anti-inflammatory activity remains to be elucidated. L-K6, an analog of temporin-1CEb isolated from the skin secretion of Rana chensinensis, has demonstrated a wide range of antimicrobial activities against gram-negative and gram-positive bacteria. In this study, the potent anti-inflammatory mechanism of L-K6 and its analogs in lipopolysaccharide (LPS)-stimulated human macrophage U937 cells were evaluated. We found that L-K6 suppressed the expression of inflammatory factors by two downstream signaling components in the MyD88-dependent pathway, including the mitogen-activated protein kinases (MAPKs) and the NF (nuclear factor)-κB signaling pathway, but its analog L-K5, which had the same amino acid sequence as L-K6 but no Lys residue at the –COOH terminal, only inhibited the phosphorylation of I-κB and NF-κB. Importantly, L-K6 and L-K5 were actively taken up by U937 cells through an independent cell membrane disruption mechanism and were eventually localized to the perinuclear region. The L-K6 uptake process was mediated by endocytosis, but L-K5 was specifically taken up by U937 cells via TLR4 endocytosis. Our results demonstrated that L-K6 can neutralize LPS and diassociate LPS micelles to inhibit LPS from triggering the proinflammatory signaling pathway, and by partially inhibiting inflammatory responses by the intracellular target. However, L-K5 may mainly inhibit proinflammatory responses by intracellular reporters to modulate the NF-κB signaling pathway.

scholarly journals Distribution of Saponins in the Sea Cucumber Holothuria lessoni; the Body Wall Versus the Viscera, and Their Biological Activities

Marine Drugs ◽  
2018 ◽  
Vol 16 (11) ◽  
pp. 423 ◽  
Author(s):  
Yadollah Bahrami ◽  
Wei Zhang ◽  
Christopher M. M. Franco
Keyword(s):  
High Performance ◽  
Sea Cucumber ◽  
Body Wall ◽  
Biological Activities ◽  
Folk Medicine ◽  
Pharmaceutical Products ◽  
The Body ◽  
Triterpene Glycosides ◽  
Partition Chromatography ◽  
Wide Range

Sea cucumbers are an important ingredient of traditional folk medicine in many Asian countries, which are well-known for their medicinal, nutraceutical, and food values due to producing an impressive range of distinctive natural bioactive compounds. Triterpene glycosides are the most abundant and prime secondary metabolites reported in this species. They possess numerous biological activities ranging from anti-tumour, wound healing, hypolipidemia, pain relieving, the improvement of nonalcoholic fatty livers, anti-hyperuricemia, the induction of bone marrow hematopoiesis, anti-hypertension, and cosmetics and anti-ageing properties. This study was designed to purify and elucidate the structure of saponin contents of the body wall of sea cucumber Holothuria lessoni and to compare the distribution of saponins of the body wall with that of the viscera. The body wall was extracted with 70% ethanol, and purified by a liquid-liquid partition chromatography, followed by isobutanol extraction. A high-performance centrifugal partition chromatography (HPCPC) was conducted on the saponin-enriched mixture to obtain saponins with a high purity. The resultant purified saponins were analyzed using MALDI-MS/MS and ESI-MS/MS. The integrated and hyphenated MS and HPCPC analyses revealed the presence of 89 saponin congeners, including 35 new and 54 known saponins, in the body wall in which the majority of glycosides are of the holostane type. As a result, and in conjunction with existing literature, the structure of four novel acetylated saponins, namely lessoniosides H, I, J, and K were characterized. The identified triterpene glycosides showed potent antifungal activities against tested fungi, but had no antibacterial effects on the bacterium Staphylococcus aureus. The presence of a wide range of saponins with potential applications is promising for cosmeceutical, medicinal, and pharmaceutical products to improve human health.

scholarly journals The Relationship between Fluid Flow, Structures, and Depositional Architecture in Sedimentary Rocks: An Example-Based Overview

Geofluids ◽  
2020 ◽  
Vol 2020 ◽  
pp. 1-19 ◽  
Author(s):  
Vilde Dimmen ◽  
Atle Rotevatn ◽  
Casey W. Nixon
Keyword(s):  
Fluid Flow ◽  
Sedimentary Rocks ◽  
Structure Type ◽  
Mineral Dissolution ◽  
Rock Properties ◽  
Hydrocarbon Migration ◽  
Geological Processes ◽  
Wide Range ◽  
Depositional Architecture ◽  
The Relationship

Fluid flow in the subsurface is fundamental in a variety of geological processes including volcanism, metamorphism, and mineral dissolution and precipitation. It is also of economic and societal significance given its relevance, for example, within groundwater and contaminant transport, hydrocarbon migration, and precipitation of ore-forming minerals. In this example-based overview, we use the distribution of iron oxide precipitates as a proxy for palaeofluid flow to investigate the relationship between fluid flow, geological structures, and depositional architecture in sedimentary rocks. We analyse and discuss a number of outcrop examples from sandstones and carbonate rocks in New Zealand, Malta, and Utah (USA), showing controls on fluid flow ranging from simple geological heterogeneities to more complex networks of structures. Based on our observations and review of a wide range of the published literature, we conclude that flow within structures and networks is primarily controlled by structure type (e.g., joint and deformation band), geometry (e.g., length and orientation), connectivity (i.e., number of connections in a network), kinematics (e.g., dilation and compaction), and interactions (e.g., relays and intersections) within the network. Additionally, host rock properties and depositional architecture represent important controls on flow and may interfere to create hybrid networks, which are networks of combined structural and stratal conduits for flow.

scholarly journals Elasticity of dense actin networks produces nanonewton protrusive forces

2021 ◽  
Author(s):  
Marion Jasnin ◽  
Jordan Hervy ◽  
Stéphanie Balor ◽  
Anais Bouissou ◽  
Amsha Proag ◽  
...  
Keyword(s):  
Actin Filaments ◽  
Actin Polymerization ◽  
Electron Tomography ◽  
Force Production ◽  
Basal Membrane ◽  
Theoretical Modelling ◽  
Elastic Behavior ◽  
Integrative Approach ◽  
Cellular Functions ◽  
Actin Networks

AbstractActin filaments assemble into force-generating systems involved in diverse cellular functions, including cell motility, adhesion, contractility and division. It remains unclear how networks of actin filaments, which individually generate piconewton forces, can produce forces reaching tens of nanonewtons. Here we use in situ cryo-electron tomography to unveil how the nanoscale architecture of macrophage podosomes enables basal membrane protrusion. We show that the sum of the actin polymerization forces at the membrane is not sufficient to explain podosome protrusive forces. Quantitative analysis of podosome organization demonstrates that the core is composed of a dense network of bent actin filaments storing elastic energy. Theoretical modelling of the network as a spring-loaded elastic material reveals that it exerts forces of up to tens of nanonewtons, similar to those evaluated experimentally. Thus, taking into account not only the interface with the membrane but also the bulk of the network, is crucial to understand force generation by actin machineries. Our integrative approach sheds light on the elastic behavior of dense actin networks and opens new avenues to understand force production inside cells.

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