scholarly journals KERAGAMAN GENOTIPE TIGA GENERASI IKAN RAINBOW KURUMOI (Melanotaenia parva) HASIL DOMESTIKASI BERDASARKAN RAPD; Genotype Diversity in Three Generations of Domesticated Kurumoi Rainbow Fish (Melanotaenia parva) Based on RAPD Method.

2016 ◽  
Vol 11 (2) ◽  
pp. 107
Author(s):  
Erma Primanita Hayuningtyas ◽  
Tutik Kadarini

Ikan rainbow Kurumoi (Melanotaenia parva) adalah ikan endemik Danau Kurumoi, Papua Barat, Indonesia. Balai Penelitian dan Pengembangan Budidaya Ikan Hias (BPPBIH), Depok telah berhasil melakukan domestikasi dan menghasilkan beberapa generasi ikan rainbow Kurumoi. Ikan rainbow Kurumoi memijah secara alami, sehingga kemungkinan terjadinya inbreeding tinggi. Oleh karena itu, sangatlah penting untuk mengevaluasi keragaman genetik ikan rainbow Kurumoi. Penelitian ini bertujuan untuk mengetahui kualitas genetik dengan mengevaluasi keragaman genotipe tiga generasi ikan rainbow Kurumoi menggunakan metode RAPD (Randomly Amplified Polymorphic DNA) dengan tiga jenis primer, yaitu: OPA-18, OPZ-5, dan OPZ-13. Setiap generasi diambil 10 ekor ikan secara acak. Hasil penelitian ditemukan bahwa keragaman genotipe ikan generasi pertama (69,25%) relatif lebih rendah (P>0,05) daripada generasi kedua (76,9%) dan ketiga (76,9%). Nilai heterozigositas cenderung meningkat dari generasi ke generasi. Heterozigositas ikan generasi pertama adalah 0,21; generasi kedua sebesar 0,24; dan generasi ketiga sebesar 0,25. Jarak genetik terjauh adalah antara generasi pertama dan generasi ketiga, yaitu sebesar 0,19. Dengan demikian, proses domestikasi yang telah dilakukan tidak menyebabkan penurunan keragaman genotipe ikan rainbow Kurumoi.Kurumoi rainbow fish (Melanotaenia parva) is an endemic species from Kurumoi Lake, West Papua, Indonesia. Research and Development for Ornamental Fish Culture, Depok has successfully domesticated and produce Kurumoi rainbow fish for several generations. This fish is breed naturally, inbreeding probability is highly occure. It is important to evaluate genetic diversity of Kurumoi rainbow fish. Aim of the research was to evaluate genotype diversity at three generations of Kurumoi rainbow fish using RAPD (Randomly Amplified Polymorphic DNA) method with three primers, namely OPA-18, OPZ-5, and OPZ-13. In the research, 10 fishes were randomly taken from each generation. The research found that genotype diversity of fish first generation (69.25%) was relatively lower (P>0.05) than second (76.9%) and third (76.9%) generations. Heterozygosity value tended to increase by generation to generation. Heterozygosity at first generation was 0.21, 0.24 at second generation and 0.25 at third generation. The highest genetic distance was between the first and third generation (0.19). Thus, the domestication process that has been done does not cause a decrease in genotype diversity of Kurumoi rainbow fish.

2013 ◽  
Vol 26 (1) ◽  
pp. 1-17
Author(s):  
Hadijah Rahmat ◽  

This paper discusses selected poems by three generations of Malay writers in Singapore from the first generation poets who received their vernicular education during British colonial period, before Malayan Independence in 1957; to second generation writers who received Malay education when Singapore was part of Malaysia, 1957-1965 who established their poems in 1970s; and the third generation writers who received bilingual education who began to make impacts when Singapore become a Republic in 1980s. These iconic poems embody the easthetic as well as the cultural and political values of Malay society. It is an early attempt to define and search for canon of Singapore Malay poetry. Keywords: literature, canon, poetry, Singapore, culture, identity, values


2012 ◽  
Vol 46 (1) ◽  
Author(s):  
Nico N. Koopman

In this article the theological contribution of Koos Vorster to human rights discourses was discussed. It was shown how he focused upon all three generations rights, namely the first generation civil and political rights, second generation social, economic and cultural rights, and third generation developmental and ecological rights. He appreciates the importance of nurturing citizens and leaders of public and civic virtue and character for the implementation of rights and the creation of a human rights culture. He also gives special attention to the implementation  of  ecological  rights  and  therefore  discussed  various  forms  of  ecocide. Ultimately, Vorster stimulates systematic theological discourses on ecological rights by viewing the implementation of ecological rights as a theological matter. It had to do with the heart of Christian faith.Aangaande die vervulling van drie generasies van regte: ‘n Teologiese bydrae deur Koos Vorster. In hierdie artikel was die teologiese bydrae van Koos Vorster tot menseregte-diskoerse bespreek. Dit dui aan dat hy op al drie generasies van menseregte gefokus het, naamlik eerste generasie burgerlike en politieke regte, tweede generasie sosiale, ekonomiese en kulturele regte en derde generasie ontwikkelings- en ekologiese regte. Hy waardeer die belangrikheid van die vorming van burgers en leiers van burgerlike en openbare deug en karakter vir die implementering van regte en die bou van ‘n menseregtekultuur. Hy het spesiale aandag gegee aan die implementering van ekologiese regte en hy het  derhalwe die verskillende vorme van die vernietiging van die natuurlike omgewing bespreek. Hy stimuleer sistematies-teologiese denke oor ekologiese regte. Hy het die implementering van ekologiese beskou as ‘n  teologiese saak. Dit het met die hart van die Christelike geloof te make gehad.


Author(s):  
M.I. Murzina ◽  

The article introduces a study in the field of monitoring the number and density of the population of grape moth using pheromone traps. In the course of this research, we found that the grape moth in 2019 developed in three generations. On the night peak flight, the largest pest population in the first generation was 2–3 insects/day; in the third generation – 1 insect/day. The population density of the grape moth in the conditions of the Novocherkassk branch of the Experimental field did not exceed the economic threshold and during the development of three generations was low. Hence, protective measures were not carried out.


2018 ◽  
Vol 12 (4) ◽  
pp. 295
Author(s):  
Otong Zenal Arifin ◽  
Wahyulia Cahyanti ◽  
Anang Hari Kristanto

Suatu penelitian untuk melihat keragaman genetik tiga generasi ikan tambakan dalam program domestikasi telah dilakukan di Balai Riset Perikanan Budidaya Air Tawar dan Penyuluhan Perikanan, Bogor. Tujuan penelitian ini adalah untuk mengevaluasi keragaman genetik generasi tetua/awal (G0), generasi pertama (G1), dan generasi kedua (G2) dalam program domestikasi ikan tambakan. Pengujian keragaman genetik dilakukan dengan metode PCR-RAPD menggunakan tiga primer, yakni OPA-2, OPA-8, dan OPC-2. Hasil yang diperoleh menunjukkan adanya perbedaan jumlah (7-15) dan ukuran fragmen (200-2.800 bp) yang dihasilkan, dengan frekuensi kemunculan alel berkisar antara 0,00-1,00 dari 31 lokus. Enam fragmen dari OPC-2 (1.400 bp, 1.300 bp, 1.100 bp, 800 bp, 600 bp, 500 bp), lima fragmen dari OPA-2 (1.350 bp, 1.000 bp, 900 bp, 800 bp, 520 bp), dan dua fragmen dari OPA-8 (1.000 bp, 550 bp) merupakan fragmen marka spesifik ikan tambakan pada penelitian ini. Keragaman genetik ikan tambakan antar generasi tergolong rendah, dengan nilai persentase polimorfisme berkisar antara 6,45%-35,48% dan nilai heterozigositas berkisar antara 0,03-0,16. Terjadi penurunan polimorfisme dan heterozigositas dari generasi tetua/awal (G0) ke generasi pertama (G1) dan kembali naik pada generasi kedua (G2). Dalam program domestikasi, nilai efektif induk (Ne) sebaiknya lebih dari 100, dengan nilai laju inbreeding (F) tidak lebih dari 0,005.A study to examine the genetic diversity of three generations of kissing gourami under a domestication program has been conducted at the Research Institute for Freshwater Aquaculture and Fisheries Extension, Bogor. The purpose of this study was to evaluate the genetic diversity of the elder generation (G0), first generation (G1), and second generation (G2) of kissing gourami under the domestication program. The genetic diversity examination was conducted through a PCR-RAPD method using three primers, namely OPA-2, OPA-8, and OPC-2. The obtained results indicated a difference in the number (7-15) and size of the fragments (200-2.800 bp) with the frequency of allele occurrence ranged from 0.00 to 1.00 from 31 loci. Six fragments from OPC-2 (1,400 bp; 1,300 bp; 1,100 bp; 800 bp; 600 bp; 500 bp), five fragments from OPA-2 (1,350 bp; 1,000 bp; 900 bp; 800 bp; 520 bp), and two fragments from OPA-8 (1,000 bp; 550 bp) were considered as species-specific markers of kissing gourami. The genetic diversity among the generations of the kissing gourami was low, with the percentage of polymorphism ranged from 6.45% to 35.48% and the value of heterozygosity ranged from 0.03 to 0.16. There was a decrease in polymorphism and heterozygosity values from the elder generation (G0) to the first generation (G1) and increased again but on the second generation (G2). In addition, the effective breeding value (Ne) in a domestication program should be more than 100, and the average of inbreeding value (F) is less than 0.005.


2020 ◽  
Vol 80 (4) ◽  
pp. 961-995
Author(s):  
Edward Kosack ◽  
Zachary Ward

We present new estimates of the outcomes of first-generation Mexicans and their descendants between 1880 and 1940. We find zero convergence of the economic gap between Mexicans and non-Mexican whites across three generations. The great-grandchildren of immigrants also had fewer years of education. Slow convergence is not simply due to an inheritance of poverty; rather, Mexican Americans had worse outcomes conditional on the father’s economic status. However, the gap between third-generation Mexican Americans and non-Mexican whites is about half the size today as it was in 1940, suggesting that barriers to Mexican American progress have significantly decreased over time.


2012 ◽  
Vol 5 (1) ◽  
pp. 97-110 ◽  
Author(s):  
Yael Darr

Since the 1990s, a new type of Holocaust story has been emerging in Israeli children's literature. This new narrative is directed towards very young children, from preschool to the first years of elementary school, and its official goal is to instil in them an authentic ‘first Holocaust memory’. This essay presents the literary characteristics of this new Holocaust narrative for children and its master narrative. It brings into light a new profile of both writers and readers. The writers were young children during the Holocaust, and first chose to tell their stories from the safe distance of three generations. The readers are their grand-children and their grand-children's peers, who are assigned an essential role as listeners. These generational roles – the roles of a First Generation of writers and of a Third Generation of readers – are intrinsically familial ones. As such, they mark a significant change in the profile of yet another important figure in the Israeli intergenerational Holocaust discourse, the agent of the Holocaust story for children. Due to the new literary initiatives, the task of providing young children with a ‘first Holocaust memory’ is transferred from the educational authority, where it used to reside, to the domestic sphere.


2020 ◽  
Vol 3 (1) ◽  
pp. 562
Author(s):  
Sylvia Margareth Sabarofek ◽  
Paulus Habel Sawor ◽  
Makarius Bajari

The purpose of this study was to study the effect of work discipline variable and simultaneous work ability and competence at the West Papua Province Social Service Office. This research is a quantitative study. The sampling method uses Non Probability Sampling and the sampling technique uses Saturated Sampling. The result of this study indicate that the variable work discipline is not significant on employee performance, and subsequent research result indicate that work ability variables partially have a siginificant effect on employee performance. Also showed a variable work discipline and work ability significantly to the performance of employess in the West Papua Province Social Service Office. Improving the performance of West papua Provincial Social Service Office employees must provide work coordination and work capabilities together to improve employee performance.


Author(s):  
Vered Noam

In attempting to characterize Second Temple legends of the Hasmoneans, the concluding chapter identifies several distinct genres: fragments from Aramaic chronicles, priestly temple legends, Pharisaic legends, and theodicean legends explaining the fall of the Hasmonean dynasty. The chapter then examines, by generation, how Josephus on the one hand, and the rabbis on the other, reworked these embedded stories. The Josephan treatment aimed to reduce the hostility of the early traditions toward the Hasmoneans by imposing a contrasting accusatory framework that blames the Pharisees and justifies the Hasmonean ruler. The rabbinic treatment of the last three generations exemplifies the processes of rabbinization and the creation of archetypal figures. With respect to the first generation, the deliberate erasure of Judas Maccabeus’s name from the tradition of Nicanor’s defeat indicates that they chose to celebrate the Hasmonean victory but concealed its protagonists, the Maccabees, simply because no way was found to bring them into the rabbinic camp.


1965 ◽  
Vol 97 (12) ◽  
pp. 1303-1318 ◽  
Author(s):  
H. J. Herbert

AbstractIn Nova Scotia one leaf cluster with an adjoining 1 inch of twig taken from the inside of each of 10 apple trees replicated four times is an adequate sample unit to measure the density of the brown mite.The brown mite has one generation with a partial second in some orchards and one with a partial second and partial third in others. The first generation adults in the bivoltine and trivoltine populations lay summer eggs on the leaves and twigs, and diapause eggs on tin twigs. The second generation adults in the bivoltine populations lay only diapause eggs; in the trivoltine populations they lay both summer and diapause eggs. The adults of the third generation lay only diapause eggs.The brown mite is found on both the leaves and woody parts of the tree. In orchards with bivoltine populations the proportion of mites on leaves reached a peak of 80% by mid-July, but thereafter gradually decreased to 10% by the end of August. However, in orchards with trivoltine populations the proportion of mites on leaves reached a peak of 80 to 90% by mid-July, remained constant until mid-August, and thereafter decreased to approximately 40% by the end of August.The number of diapause eggs laid by adults of each generation in both the bivoltine and trivoltine populations varies widely. The eggs are deposited on the trunk as well as on the branches, with the heaviest deposition in the central area of the tree. The diapause eggs laid by adults of the first generation are the last to hatch and those laid by the third generation are the first to hatch the following spring.The factors responsible for the differences in the number of generations and in the number of diapause eggs laid are unknown.


Author(s):  
Yeşim Sevinç

AbstractDrawing on questionnaire and interview data, this study explores the process of language maintenance and shift across three generations of Turkish immigrants in the Netherlands. It compares three generations of Turkish-Dutch bilinguals by examining age and place of language learning, self-rated language proficiency, and language choices in six domains (home, school, work, friends, media and leisure time activities, and cognitive activities). Furthermore, it investigates bilinguals’ experiences, motivations for learning languages and attitudes towards bilingualism. Findings suggest that following the typical pattern of language shift described by Mario Saltarelli and Susan Gonzo in 1977, language history, self-rated language proficiency and current language practices of third-generation children differ from those of first- and second-generation bilinguals. Consequently, possible language shift among third-generation bilinguals causes socioemotional pressure about maintaining the Turkish language, triggering intergenerational tensions in Turkish immigrant families. At the same time, the perceived need to shift to Dutch for social and economic reasons causes immigrant children to experience tensions and ambiguities in the linguistic connections between the family and other social domains (e. g. school, friendship). The findings evidence that the Turkish immigrant community in the Netherlands may no longer be as linguistically homogeneous as once observed. The dissolution of homogeneity can be a sign of social change in which maintaining the Turkish language has become a challenge, whereas speaking Dutch is a necessity of life in the Netherlands.


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