Theoretical justification of selecting a method for cultivation of mammal cells as a basis for designing membrane bioreactors in accordance with the requirements of good manufacturing practice

The object of research is technological cultivation systems, the central element of which are bioreactors, which ensure efficient metabolism of mammalian cells (Metazoa). Fundamental differences in the phenotypic characteristics of mammalian cells from microorganisms and the special phase-hydrodynamic state of the cultivation system form special requirements for the design and operation of bioreactors. One of the most problematic areas in the process of using cell cultures to obtain medicinal products of biological origin is to ensure the correct cultivation conditions in order to obtain the maximum amount of the target product. The quality assurance system for drug production is based on good manufacturing practice, which puts forward a number of general requirements for the organization of production of drugs of specified quality, efficacy and safety. The study analyzed the phenotypic characteristics of mammalian cells. The analysis of industrial cultivation systems is carried out and a new classification is proposed, which takes into account the phase-hydrodynamic state of the culture and the specifics of the hydrodynamics of the bioreactor. The analysis made it possible to determine membrane cultivation systems as the most promising. The choice of this cultivation system has a number of features, in particular, the provision of favorable conditions during a long process of cultivation of support-dependent cells. This system guarantees a constant and efficient supply of nutrients, including dissolved oxygen, and the removal of waste products. A wide range of materials for the manufacture of membranes allows to use an individual approach to different types of cell lines. Due to this, it is ensured that high cultivation rates are obtained - the density of cells on the growth surface, the provision of the necessary substances during the entire cultivation process without negative mechanical effects on the cells. The modular design of the membrane elements allows for scalability of the cultivation process from laboratory development to industrial cultivation. The results obtained in this work can be the initial data in the study of hydrodynamic and mass transfer characteristics and in the development or construction of new bioreactors, which will reduce the stage of laboratory development and accelerate the introduction of innovative drugs.

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Evaluation of the nucleopolyhedrovirus of Anticarsia gemmatalis as a vector for gene therapy in mammals

Current Gene Therapy ◽

10.2174/1566523220999201217155945 ◽

2020 ◽

Vol 20 ◽

Author(s):

Cintia N. Parsza ◽

Diego L. Mengual Gómez ◽

Jorge Alejandro Simonin ◽

Mariano Nicolás Belaich ◽

Pablo Daniel Ghiringhelli

Keyword(s):

Gene Therapy ◽

Mammalian Cells ◽

Insect Cells ◽

Autographa Californica ◽

Anticarsia Gemmatalis ◽

Agricultural Pests ◽

Mammalian Cell Lines ◽

Delivery Vector ◽

Wide Range ◽

Insect Pathogens

Background: Baculoviruses are insect pathogens with important biotechnological applications that transcend their use as biological controllers of agricultural pests. One species, Autographa californica multiple nucleopolhyedrovirus (AcMNPV) has been extensively exploited as a molecular platform to produce recombinant proteins and as a delivery vector for genes in mammals, because it can transduce a wide range of mammalian cells and tissues without replicating or producing progeny. Objective/Method: To investigate if the budded virions of Anticarsia gemmatalis multiple nucleopolhyedrovirus (AgMNPV) species has the same ability, the viral genome was modified by homologous recombination into susceptible insect cells to integrate reporter genes and then it was evaluated on mammalian cell lines in comparative form with respect to equivalent viruses derived from AcMNPV. Besides, the replicative capacity of AgMNPV´s virions in mammals was determined. Results: The experiments carried out showed that the recombinant variant of AgMNPV transduces and support the expression of delivered genes but not replicates in mammalian cells. Conclusion: Consequently, this insect pathogen is proposed as an alternative of non-infectious viruses in humans to explore new approaches in gene therapy and other applications based on the use of mammalian cells.

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The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry

Open Life Sciences ◽

10.1515/biol-2020-0099 ◽

2020 ◽

Vol 15 (1) ◽

pp. 787-796 ◽

Cited By ~ 1

Author(s):

Marek Kieliszek ◽

Kamil Piwowarek ◽

Anna M. Kot ◽

Katarzyna Pobiega

Keyword(s):

Food Industry ◽

Industrial Applications ◽

Waste Products ◽

Yeast Biomass ◽

Wide Range ◽

Innovative Solutions ◽

Biomass Management ◽

Effective Use ◽

Modern Technologies ◽

Biomass Of Microorganisms

AbstractCellular biomass of microorganisms can be effectively used in the treatment of waste from various branches of the agro-food industry. Urbanization processes and economic development, which have been intensifying in recent decades, lead to the degradation of the natural environment. In the first half of the 20th century, problems related to waste management were not as serious and challenging as they are today. The present situation forces the use of modern technologies and the creation of innovative solutions for environmental protection. Waste of industrial origin are difficult to recycle and require a high financial outlay, while the organic waste of animal and plant origins, such as potato wastewater, whey, lignin, and cellulose, is dominant. In this article, we describe the possibilities of using microorganisms for the utilization of various waste products. A solution to reduce the costs of waste disposal is the use of yeast biomass. Management of waste products using yeast biomass has made it possible to generate new metabolites, such as β-glucans, vitamins, carotenoids, and enzymes, which have a wide range of industrial applications. Exploration and discovery of new areas of applications of yeast, fungal, and bacteria cells can lead to an increase in their effective use in many fields of biotechnology.

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Autophagy Deficiency by Atg4B Loss Leads to Metabolomic Alterations in Mice

Metabolites ◽

10.3390/metabo11080481 ◽

2021 ◽

Vol 11 (8) ◽

pp. 481

Author(s):

Gemma G. Martínez-García ◽

Raúl F. Pérez ◽

Álvaro F. Fernández ◽

Sylvere Durand ◽

Guido Kroemer ◽

...

Keyword(s):

Skeletal Muscle ◽

Amino Acids ◽

Mammalian Cells ◽

Tissue Homeostasis ◽

In Vivo Studies ◽

Protective Mechanism ◽

Cardiac Damage ◽

Wide Range ◽

First Time

Autophagy is an essential protective mechanism that allows mammalian cells to cope with a variety of stressors and contributes to maintaining cellular and tissue homeostasis. Due to these crucial roles and also to the fact that autophagy malfunction has been described in a wide range of pathologies, an increasing number of in vivo studies involving animal models targeting autophagy genes have been developed. In mammals, total autophagy inactivation is lethal, and constitutive knockout models lacking effectors of this route are not viable, which has hindered so far the analysis of the consequences of a systemic autophagy decline. Here, we take advantage of atg4b−/− mice, an autophagy-deficient model with only partial disruption of the process, to assess the effects of systemic reduction of autophagy on the metabolome. We describe for the first time the metabolic footprint of systemic autophagy decline, showing that impaired autophagy results in highly tissue-dependent alterations that are more accentuated in the skeletal muscle and plasma. These changes, which include changes in the levels of amino-acids, lipids, or nucleosides, sometimes resemble those that are frequently described in conditions like aging, obesity, or cardiac damage. We also discuss different hypotheses on how impaired autophagy may affect the metabolism of several tissues in mammals.

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Continuum modeling perspectives of non-Fourier heat conduction in biological systems

Journal of Non-Equilibrium Thermodynamics ◽

10.1515/jnet-2021-0016 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Ákos Sudár ◽

Gergely Futaki ◽

Róbert Kovács

Keyword(s):

Biological Systems ◽

Phase Lag ◽

Dual Phase ◽

Continuum Modeling ◽

Biological Origin ◽

Ultrasound Surgery ◽

Thermal Models ◽

Wide Range ◽

Fourier Heat Conduction ◽

The Continuum

Abstract The thermal modeling of biological systems is increasingly important in the development of more advanced and more precise techniques such as ultrasound surgery. One of the primary barriers is the complexity of biological materials: the geometrical, structural, and material properties vary in a wide range. In the present paper, we focus on the continuum modeling of heterogeneous materials of biological origin. There are numerous examples in the literature for non-Fourier thermal models. However, as we realized, they are associated with a few common misconceptions. Therefore, we first aim to clarify the basic concepts of non-Fourier thermal models. These concepts are demonstrated by revisiting two experiments from the literature in which the Cattaneo–Vernotte and the dual phase lag models are utilized. Our investigation revealed that these non-Fourier models are based on misinterpretations of the measured data, and the seeming deviation from Fourier’s law originates from the source terms and boundary conditions.

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A novel human Smad4 mutation is involved in papillary thyroid carcinoma progression

Endocrine Related Cancer ◽

10.1530/erc-11-0233 ◽

2011 ◽

Vol 19 (1) ◽

pp. 39-55 ◽

Cited By ~ 15

Author(s):

Sonia D'Inzeo ◽

Arianna Nicolussi ◽

Caterina Francesca Donini ◽

Massimo Zani ◽

Patrizia Mancini ◽

...

Keyword(s):

Papillary Thyroid Carcinoma ◽

Thyroid Carcinoma ◽

Mammalian Cells ◽

Transforming Growth Factor ◽

Human Cancer ◽

Papillary Thyroid ◽

Tgfβ Signaling ◽

Strong Reduction ◽

Mesenchymal Transition ◽

Wide Range

Smad proteins are the key effectors of the transforming growth factor β (TGFβ) signaling pathway in mammalian cells. Smad4 plays an important role in human physiology, and its mutations were found with high frequency in wide range of human cancer. In this study, we have functionally characterized Smad4 C324Y mutation, isolated from a nodal metastasis of papillary thyroid carcinoma. We demonstrated that the stable expression of Smad4 C324Y in FRTL-5 cells caused a significant activation of TGFβ signaling, responsible for the acquisition of transformed phenotype and invasive behavior. The coexpression of Smad4 C324Y with Smad4 wild-type determined an increase of homo-oligomerization of Smad4 with receptor-regulated Smads and a lengthening of nuclear localization. FRTL-5 clones overexpressing Smad4 C324Y showed a strong reduction of response to antiproliferative action of TGFβ1, acquired the ability to grow in anchorage-independent conditions, showed a fibroblast-like appearance and a strong reduction of the level of E-cadherin, one crucial event of the epithelial–mesenchymal transition process. The acquisition of a mesenchymal phenotype gave the characteristics of increased cellular motility and a significant reduction in adhesion to substrates such as fibronectin and laminin. Overall, our results demonstrate that the Smad4 C324Y mutation plays an important role in thyroid carcinogenesis and can be considered as a new prognostic and therapeutic target for thyroid cancer.

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Nocodazole and cytochalasin D induce tetraploidy in mammalian cells

AJP Cell Physiology ◽

10.1152/ajpcell.1984.246.1.c154 ◽

1984 ◽

Vol 246 (1) ◽

pp. C154-C156 ◽

Cited By ~ 21

Author(s):

G. W. Zieve

Keyword(s):

Cell Division ◽

Mammalian Cells ◽

Cytochalasin D ◽

Microtubule Assembly ◽

Reversible Inhibitor ◽

Cleavage Furrow ◽

Synchronized Cells ◽

Cells In Culture ◽

Wide Range

Nocodazole, a rapidly reversible inhibitor of microtubule assembly is useful for preparing mammalian cells synchronized at all stages of mitosis. When synchronized cells are allowed to progress through mitosis in the presence of cytochalasin D, the cleavage furrow is inhibited and dikaryon cells are formed. These cells become homogeneous populations of stable mononuclear tetraploid cells after the following cell division. This procedure is applicable to a wide range of mammalian cells in culture.

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Reuse of Treated Municipal Wastewater from Constructed Wetlands for Cut Flowers Irrigation in Aeroponic Cultivation

Ingenieria y Universidad ◽

10.11144/javeriana.iued24.tmwr ◽

2020 ◽

Vol 24 ◽

Author(s):

Ismael Leonardo Vera-Puerto ◽

Jorge Leonardo Olave-Vera ◽

Sussy Tapia ◽

Wladimir Antonio Chávez ◽

Carlos Arias

Keyword(s):

Constructed Wetlands ◽

Municipal Wastewater ◽

Cut Flower ◽

Cut Flowers ◽

Cultivation System ◽

Cultivation Systems ◽

Arid Conditions ◽

Affected Plant ◽

Treated Municipal Wastewater

The aim of this work is to evaluate the reuse of municipal wastewaters treated through subsurface constructed wetlands (SS-CWs) as irrigation water in cut flower aeroponic cultivation under arid conditions. For this purpose, two experimental aeroponic cultivation systems were installed with the cut flower Lilium ‘Tresor’ planted and irrigated with SS-CWs treated water. The results showed that the quality of the SS-CWs wastewater has to be improved to be used in irrigation. Despite that, Lilium ‘Tresor’ grew under arid conditions with normal stem diameters and number of flowers but with heights under 0.65 m, which would restrict their commercialization to local markets. Water electrical conductivity (> 2300 µs/cm) and luminosity (> 120 klux) were factors that affected plant height. When compared to other cultivation systems, the aeroponic cultivation system used between 10 % and 20 % of the amount of water needed to produce Lilium ‘Tresor.’ Thus, this work showed the feasibility to produce cut flowers using an aeroponic cultivation system under arid conditions and irrigated with SS-CWs effluents. Likewise, it was detected that improvements to water quality and luminosity must be made for industrial scaling.

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Use of peroxiredoxin for preconditioning of heterotopic heart transplantation in a rat

Russian Journal of Transplantology and Artificial Organs ◽

10.15825/1995-1191-2020-2-158-164 ◽

2020 ◽

Vol 22 (2) ◽

pp. 158-164

Author(s):

N. V. Grudinin ◽

V. K. Bogdanov ◽

M. G. Sharapov ◽

N. S. Bunenkov ◽

N. P. Mozheiko ◽

...

Keyword(s):

Heart Transplantation ◽

Reperfusion Injury ◽

Mammalian Cells ◽

Ischemia Reperfusion Injury ◽

Isolated Heart ◽

Ischemia Reperfusion ◽

Signal Transmission ◽

Redox Homeostasis ◽

Heterotopic Heart Transplantation ◽

Wide Range

Peroxiredoxin 6 (Prdx6) is an antioxidant enzyme in the human body that performs a number of important functions in the cell. Prdx6 restores a wide range of peroxide substrates, thus playing a leading role in maintaining redox homeostasis in mammalian cells. In addition to peroxidase activity, Prdx6 has an activity of phospholipase A2, thus taking part in membrane phospholipid metabolism. Due to its peroxidase and phospholipase activity, Prdx6 participates in intracellular and intercellular signal transmission, thereby facilitating the initiation of regenerative processes in the cell, suppression of apoptosis and activation of cell proliferation. Given the functions performed, Prdx6 can effectively deal with oxidative stress caused by various factors, including ischemia-reperfusion injury. On an animal model of rat heterotopic heart transplantation, we showed the cardioprotective potential of exogenous recombinant Prdx6, introduced before transplantation and subsequent reperfusion injury of the heart. It has been demonstrated that exogenous Prdx6 effectively alleviates the severity of ischemia-reperfusion injury of the heart by 2–3 times, providing normalization of its structural and functional state during heterotopic transplantation. The use of recombinant Prdx6 can be an effective approach in preventing/alleviating ischemia-reperfusion injury of the heart, as well as in maintaining an isolated heart during transplantation.

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ANALISIS PERTUMBUHAN Sargassum sp. DENGAN SISTEM BUDIDAYA YANG BERBEDA DI TELUK EKAS LOMBOK TIMUR SEBAGAI BAHAN PENGAYAAN MATA KULIAH EKOLOGI TUMBUHAN

JURNAL BIOLOGI TROPIS ◽

10.29303/jbt.v15i2.157 ◽

2015 ◽

Vol 15 (2) ◽

Author(s):

Muhammad Lutfiawan, Karnan Dan Lalu Japa

Keyword(s):

Computer Program ◽

Experimental Research ◽

T Test ◽

Program Package ◽

Cultivation System ◽

Significance Level ◽

Cultivation Systems ◽

Floating Raft ◽

Probability Table ◽

Growth Differences

ABSTRAKTujuan dari penelitian ini adalah untuk mengetahui perbedaan pertumbuhan Sargassum sp.yang dibudidaya dengan sistem budidaya yang berbeda di Teluk Ekas Lombok Timur. Jenispenelitian ini adalah penelitian eksperimen.. Sampel dalam penelitian ini adalah tanamanSargassum sp yang dibudidayakan dengan sistem budidaya yang berbeda yaitu : rakitterapung dan rakit patok dasar. Pengolahan data dilakukan dengan menggunakan paketprogram komputer pengolahan data yaitu SPSS 16. Pengujian hipotesis dilakukan denganmenggunakan uji-t polled varians, dengan taraf signifikan 0,05 dan taraf kepercayaan 95 %,diperoleh t-hitung 11,974 > t-tabel 1,987, atau berdasarkan besarnya nilai probabilitas yangditunjukan, diperoleh probabilitas-hitung 0,00 < probabilitas-tabel 0,05, sehingga Ho ditolakdan Ha diterima, ini artinya terdapat perbedaan pertumbuhan Sargassum sp. yang dibudidayadengan sistem budidaya yang berbeda.Kata-kata kunci: Sargassum sp., Teluk Ekas, Sistem budidaya yang berbeda.ABSTRACTThe purpose of this research is to determine growth differences of Sargassum sp., werecultured with different cultivation systems in the Ekas Bay of East Lombok. This research isan experimental research. The sample in this research is Sargassum sp. of cultivated cropswith different cultivation systems, such as : floating raft and raft basic stakes. Processing datewas performed by using a processing date computer program package SPSS 16. Hypothesistesting is done by using t-test polled variance, with a significance level of 0.05 and a level ofvalid 95 %, in order to obtain t count 11.974 > t - table 1,987, or based on the value of theindicated probability, probability-count obtained 0.00 < 0.05 probability- table , so that Ho isrejected and Ha accepted , this means that there are differences in the growth of Sargassumsp. were cultured with different cultivation systems.Key words: Sargassum sp., Ekas bay, Different Cultivation System.

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Production of Light Weight Blocks Using Rice Husk

International Journal of Environment and Climate Change ◽

10.9734/ijecc/2021/v11i930473 ◽

2021 ◽

pp. 24-30

Author(s):

C. U. Okpoechi ◽

C. A. Emma-Ochu ◽

L. C. Ibemere ◽

P. Ibe ◽

E. O. Onwuka ◽

...

Keyword(s):

Rice Husk ◽

Affordable Housing ◽

Low Income ◽

Waste Product ◽

Housing Construction ◽

Management Problem ◽

Solid Core ◽

Structural Walls ◽

Waste Products ◽

Wide Range

Affordable housing has remained a major challenge in Nigeria, as housing costs have continued to rise beyond the reach of the low income population. This paper explores the use of waste products like rice husk as alternative materials for housing construction. Rice husk is abundant as a waste product in areas where rice is processed commercially in Southeast Nigeria. The aim of the study is to create rice husk blocks that are cheap, lightweight, and appropriate for use in low income housing construction. The study was conducted through experimentation in the Building Technology workshop of Federal Polytechnic Nekede. A series of trial mixes were done involving a wide range of materials and mix proportions. Rice husk, Portland cement, and cassava starch, were found to be the most appropriate components for the blocks. The blocks produced were of good appearance, and lightweight. Five samples of solid core blocks measuring 150x150x150mm were tested in Strength of Materials laboratory of the Federal University of Technology Owerri. The average compressive strength of the blocks was 0.26N/mm2, which is below the Nigerian Industrial Standard NIS 87:2007. The blocks produced were considered appropriate for use as non-load bearing partitions and not structural walls. The result of the study is promising because the rice husk blocks help address the waste management problem in affected areas, and could also be an important component of a potentially useful material. This paper recommends further research in the area of improving the strength of the blocks, to make them usable as structural components in low rise buildings.

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