Seasonal Dynamics of Organic Carbon and Nitrogen in Biomasses of Microorganisms Affected by Different Tillage Systems

The main purpose of this study was to determine the size and direction of the seasonal dynamics of organic carbon (Сmicro) and nitrogen (Nmicro) biomass of microorganisms and microbial index (Cmicro : Corg) of natural and agrocenoses with their different uses. Field research methods involved taking of soil samples in 0-10-, 10-20- and 20-40 сm layers. Under laboratory conditions, the content of total soil carbon was determined by dry oxygen combustion on a Vario EL III analyzer (Elementar Analyzensysteme, Hanau, Germany). The carbon content of microbial biomass (Сmicro) was determined by chloroform fumigation extraction method (CFE). To freshly taken soil samples (2 hours) and soil samples after their 24-hour fumigation with chloroform vapors, 0.5 M K2SO4 was added to extract biomass lysis products of soil microorganisms. The content of organic carbon and nitrogen in the biomass of microorganisms in the obtained filtrates was determined on the Elementar Liqui TOC II, Analyzensysteme GmbH, Germany. The carbon content of microbial biomass was calculated from the difference between carbon in fumigated and control samples using a factor of 0,45 - for carbon and 0,54 – for nitrogen. The microbial index of soils was determined by the ratio between the carbon of microorganisms and the total organic carbon of the soil – Cmicro : Corg • 100 (%). Average values and confidence intervals were determined for each defined indicator. The Bonferoni method was used to correct the errors of multiple comparative samples of a one-way ANOVA analyze. K. Pearson’s linear correlation analysis was used to establish the relationships between the dynamics of carbon biomass of microorganisms and organic carbon of the soil during the growing season. Our research has shown the dynamics of Сmicro, Nmicro, Сmicro : Nmicro and Cmicro : Corg during the growing season. Analysis of the box plot showed the largest amplitude of Сmicro changes in the upper 0-10 cm layer of izogumusol. The smallest difference in the quartile range (IQR0,25-0,75) was for no-till and overhang (Ab) in the upper 0-10-, no-till (NT) and fallow (F) - in the layer 10-20- and plowing (CT) - in a layer of 20-40 cm. The content of organic carbon biomass of microorganisms in the upper layer of izogumusol at the beginning of the growing season had the highest values of Ab (577,79  1,64 mg/kg), NT (485,43  1,97 mg/kg) and CT (470,43  0,77 mg/kg), the smallest - for F (370,15  2,18 mg/kg). The content of Nmicro during this period decreased from Ab to Comb (combined tillage), NT, CT, Rot (rotary tillage), RT (reduced (ridge) tillage) and F, respectively. In the 20-40 cm layer, the highest values of Сmicro and Nmicro were observed in mid-July. The lowest values of Сmicro and Nmicro and the largest – Сmicro : Nmicro were found in late August for all variants and layers of the study. The dynamics of the microbial index resembled the trends of Сmicro and Nmicro. The largest share of Smicro in Sorghum during the growing season, on average was: - Ab (1,82  1,85 %) and NT (1,66  1,52 %) - in the layer 0-10-, - Ab (1,23  1,27 %) and NT (1,29  1,32 %) - in the layer 10-20- and - Ab (1,19  1,09 %) and F (1,11  1,077 %) - in a layer of 20-40 cm. Different use of izogumusol affected the amplitude of seasonal changes of Сmicro and Nmicro and did not affect on their direction. The maximum content of Сmicro and Nmicro was observed at the beginning of the growing season - in a layer of 0-10 cm and in mid-July - in a layer of 20-40 cm, the minimum - at the end of the summer period. During this period, the widest ratio of Сmicro : Nmicro was for F and CT - in the layer 0-20 cm and CT and Rot - in the layer 20-40 cm. The Pearson’s correlation coefficient between Сmicro and Corg increased from the upper 0-10- to the lower 20-40 cm layer of izogumusol. "Strong" and "high" negative correlations have been established between Сmicro and Corg, but no pattern has been found between the correlation coefficient and tillage technologies.

Biogeochemical processes in the Barents sea

Biogeochemical processes involving microorganisms play an important role in marine sedimentogenesis. The study of biogeochemical processes in the Barents Sea was carried out from 1997 with interruptions until 2019. Using a complex of geological-geochemical, microbiological, radioisotope and stable isotope methods, it was possible to obtain a quantitative estimate of the total abundance and biomass of microorganisms, rates of biogeochemical processes, methane content and organic matter suspended. In the course of work in four expeditions, it was found that in the surface (0–10 m) water column south of 74° N the magnitude of the total abundance and the biomass of microorganisms increased by 2019 by about 5 times compared to 1998. To the north, in colder waters, the total abundance and the biomass of organisms were lower than in the southern region of the sea. The methane concentration in the surface layer of the water column at the border with the atmosphere did not change much for 20 years (1976–1997) and increased noticeably from 1997 to 2017, from 3.3 to 15.8 nM. The increase in FFM, the biomass of organisms and the concentration of methane in the water column is associated with the melting of glaciers, with the release of organic matter of continental origin released from ice into the water. The results of the work indicate changes in the ecosystem of the Barents Sea.

A first assessment of the microbiota of Taurida Cave

Caves are considered as ecosystems isolated from the surface in varying degrees. Hypogean habitats are mostly oligotrophic, with discretely distributed nutrients, where chemolithoautotrophic species can be found among the producers. In this case, vital activity is provided due to the nutrients of endogenous genesis. Of particular interest are the cavities, which were completely isolated from the surface impact for a long time. As a consequence, unique landscapes and mineral environments were formed in such cavities. An example is given by Taurida Cave, located on the Crimean Peninsula (Piedmont Crimea) and discovered during the construction of Taurida Highway. Samples of sediments were taken right after opening the cave in July 2018. For the cultivation of bacteria andmicrofungi, standard media, extracts from substrates were used. The number and biomass of microorganisms were determined by luminescence microscopy. Chemical composition of the main and trace elements of the cave deposits samples was determined by XRF WDS spectrometer. As a result, a difference in the number and biomass of microorganisms in different parts of the cave was revealed. The main contribution to the biomass of microorganisms is made by actinomycetes and microfungi. The bacteria were dominated by gram-positive bacteria of the genera Bacillus, Arthrobacter, Micrococcus. Among actinomycetes, species of the genus Streptomyces predominated. The species of microfungi Penicillium chrysogenum, Trichoderma sp., Aspergillus sp. were identified, Penicillium janczewskii dominated. The high abundance and biomass of microorganisms in the substrates of the cave may be related to the summer sampling period.

The aspects of microbial biomass use in the utilization of selected waste from the agro-food industry

Cellular biomass of microorganisms can be effectively used in the treatment of waste from various branches of the agro-food industry. Urbanization processes and economic development, which have been intensifying in recent decades, lead to the degradation of the natural environment. In the first half of the 20th century, problems related to waste management were not as serious and challenging as they are today. The present situation forces the use of modern technologies and the creation of innovative solutions for environmental protection. Waste of industrial origin are difficult to recycle and require a high financial outlay, while the organic waste of animal and plant origins, such as potato wastewater, whey, lignin, and cellulose, is dominant. In this article, we describe the possibilities of using microorganisms for the utilization of various waste products. A solution to reduce the costs of waste disposal is the use of yeast biomass. Management of waste products using yeast biomass has made it possible to generate new metabolites, such as β-glucans, vitamins, carotenoids, and enzymes, which have a wide range of industrial applications. Exploration and discovery of new areas of applications of yeast, fungal, and bacteria cells can lead to an increase in their effective use in many fields of biotechnology.

Changes in the microbiocenosis of the rumen and digestibility of the dry matter of the diet with the introduction of gobies together fatty addition of ultrafine iron particles

The gastrointestinal tract of cattle is a large and complex biomechanism for processing substances coming from outside, with their further assimilation by the animal’s body, to ensure normal life and productive growth. A special role is played by the rumen of cattle, in which the biosynthetic processes of the conversion of some chemicals and elements to others, under the action of enzymes of microorganisms, which have a responsible role in the processing of food components, as well as in a symbiotic relationship to each other. So, a disruption in the work of certain microorganisms under the influence of food factors leads to a disruption in the synthesis and work of the rest of the microbiota, which generally affects the state of the animal bioreactor as a whole. The aim of the study was to establish the effect of various dosages of ultrafine particles of Fe in combination with a fat supplement, as part of rations on the digestibility of dry matter of the feed, basic indicators of scar content, such as microbial fermentation of the feed and biomass of microorganisms and fatty acid composition. The scientific novelty of the work lies in the fact that the effect of a complex of ultrafine particles of iron of different dosages ϲ a fat supplement on the cicatricial digestion of bulls raised for meat was studied. Methodology and methods. To conduct research, 12 bulls were bred in the conditions of the Pokrovskiy agricultural college-branch of the Orenburg State Agrarian University of the Orenburg region of the Orenburg region. Of these, four groups were selected according to the principle of analogues – control and three experimental, three animals in each, which, according to A. A. Aliyev were imposed scar fistulas. Results and scope. Thus, during the experiment it was found that the optimal dosage of iron nanoparticles mixed with a fat supplement in the diet was 425.6 mg per head per day, which contributes to better digestibility of dry matter by 8.98 % and a higher content of VFA in scar fluid by 2.28 % compared to ϲ control.

Formation of the structure of microbiocenoses of soils of agroecosystems depending on trophic and hydrothermal factors

Ground soil, as the product primarily of the activity of microbiota, is under the permanent influence of ecological and anthropogenous factors. Soils are especially subject to pressure in agroecosystems, which increases due to the considerable fluctuation of climate system parameters. Using graph analysis, we have estimated the results of multiyear monitoring research on the functioning of microbiocenoses of three soil types in agroecosystems depending on the fertilizing and hydrothermal mode. It enabled us to detect peculiarities of formation of the structure soil microbiocenoses and to better understand ecologically important relations between functional groups of microorganisms in the soil depending on action of different factors. It has been determined that neither application of organic and mineral fertilizers into chernozem nor action of hydrothermal factors (temperature air and humidity) is a crucial characteristic of changing in the structure of its microbiocenosis. Microorganisms-producers of exopolysaccharides, which have a strong relationship with all trophic groups of microorganisms as they are their structural components, turned out to be the main block constructing factor, which is due to their strong influence on total content of microbal mass in soil. For soil of agroecosystems with dark-grey type of soil characteristic of content of total biomass of microorganisms is a basis for block constructing gremium, which affirms the state of microbiocenosis and processes occurring there under the action of researched biotic and abiotic factors. Microbiocenosis of sod-podzolic soil, unlike the other researched types of soils, reacted distinctly on applying of fertilizer and depended on the action of hydrothermal factors. Contrast in the range of hydrothermal regime caused a chaotic character of interaction between the basic characteristics in microbiocenosis of sod-podzolic soil with appearance of a direct and mediated relationship among them. Regardless of changes in hydrothermal factors , interactions between characteristics of total microbial mass content, eutrophic microorganisms, which use mineral and organic forms of nitrogen, and producers of exopolysaccharides were stable. It has been proved that estimating of ecological state of soil and influence of applicable agromeasures on it should be conducted according to the indices of total microorganism biomass content in the soil and correlation between the number of microorganisms which form the graph gremium.

Technology of production biological active additive based on selenium containing culture of bifidobacterium

The article presents data on the positive impact of essential microelement selenium on the human body. It was characterized the ability to accumulate inorganic forms of selenium (such as selenites and selenates) into the organic forms by probiotic microorganisms. The article presents data concerning sodium selenite concentration impact on biomass growth of bifidobacterium culture. It was fined optimum conditions for accumulation maximum selenium containing biomass of microorganisms. Based on experiments it was created selenium containing biologically active additive.

Air Basin Protection in the Bulk Manufacturing of Fodder Protein

Biomass of microorganisms in aerogenic dissemination is an allergen and causes bronchopulmonary pathology. Organized source of air-gas emissions (AGE) in the production of protein—vitamin additives of any kind of raw materials are driers, bioreactors and separators. There were provided the results of a survey of a number of AGE production. There were developed new types of gas treatment equipment – vortex absorber and a mesh mist separator with continuous regeneration for bioreactors and separators. The greatest contribution to the AGE of fodder protein production make drying compartments. New technical solutions for their technological improvement did not on the path of increasing of gas treatment equipment capacity, but on the path to change hardware technological scheme. There were developed and tested a few schemes with varying degrees of closure of drying agent’s (DA) circulation. The final decision was the scheme with a fully closed-loop circulation of DA, thus ensuring the full environmental safety and explosion-proof of drying compartments.