scholarly journals Relationship between scrotal circumference and semen parameters in crossbred bulls

1970 ◽  
Vol 26 (2) ◽  
pp. 61-67 ◽  
Author(s):  
MA Latif ◽  
JU Ahmed ◽  
MMU Bhuiyan ◽  
M Shamsuddin

The relationship between the scrotal circumference and semen volume, sperm concentration and number of sperm per ejaculate of 12 crossbred bulls [4 Local × Friesian, 4 Local × Sahiwal] was studied. Semen of three consecutive collections at seven day intervals was evaluated. The age of bulls at first collection varied between 15 and 20 months. Soon after collection, volume, sperm concentration, number of spermatozoa per ejaculate and initial sperm motility of fresh semen were measured. Scrotal circumference was correlated with semen volume, sperm concentration and number of spermatozoa per ejaculate in both groups. A significant (P<0.04) positive correlation (r = 0.72) was observed between scrotal circumference and volume of semen, and between scrotal circumference and number of sperm production per ejaculate. Significant (P<0.05) variation was observed in different semen parameters between bulls within the same group, but no significant (P>0.05) variation was found between the two groups. It is suggested that crossbred bulls aged 18 months or over, with scrotal circumference more than 30 cm, yielded good quality semen. DOI: 10.3329/bvet.v26i2.4952 Bangl. vet. 2009. Vol. 26, No. 2, 61-67

2021 ◽  
Vol 19 (2) ◽  
pp. 237-244
Author(s):  
Nguyen Huu Duc ◽  
Pham Thu Giang ◽  
Tran Thi Binh Nguyen ◽  
Nguyen Thi Mai ◽  
Bui Dai Phong

The objective of this study was to determine the semen cryopreservation capacity of BBB bulls in Hanoi-Vietnam. Research conducted on the fresh semen collected from 05 BBB bulls. Results showed that semen color was normal (milky white, ivory white, ivory yellow), semen volume ranged from 6.35 mL to 7.48 mL (P <0.05), initial motility of semen ranged from 80.53% to 82.92% (P <0.05), sperm concentration in semen  ranged from 1.02 x 109 sperms/ml to 1.12 x 109 sperms/mL (P <0.05), abnormal sperm ratio ranged from 6.45% to 8.12% (P <0.05), alive sperm ratio ranged from 76.34% to 82.97% (P <0.05), sperm motility after thawing from straw semen ranged from 71.33% to 75.92% (P<0.05). In conclusion, successfully semen collection from 05 breeding BBB bulls at Hanoi Cattle Breeding Joint Stock Company, semen samples had normal color and good quantity and quality, suitable for production of frozen semen; and semen cryopreservation of straws of the 05 bull BBB semen mentioned at -196oC, sperm motility after freezing-thawing reached the economic and technical norms of 675/2014 of the Ministry of Agriculture and Rural Development.


2015 ◽  
Vol 49 (6) ◽  
Author(s):  
S Frydrychová ◽  
A Lustyková ◽  
E Václavková ◽  
J Lipenský ◽  
M Rozkot

The objective of this study was to investigate the effect of using different extenders <italic>viz.</italic> Androhep, Safecell Plus and SUS during cryopreservation on quality of frozen-thawed boar semen. Semen volume, sperm motility, sperm concentration, percentage of morphologically abnormal spermatozoa, total number of spermatozoa per ejaculate and activity of the enzyme aspartate aminotransferase (AST) were assessed in fresh semen collected from 39 fertile AI boars. Semen from each boar was divided into three portions and diluted 1:1.5 in extender Androhep, Safecell Plus and SUS and keep at 17°C for 15-h holding time before cryopreservation. Then sperm was cryopreserved. Straws were thawed in a water bath at 38°C for 40s and post-thaw sperm motility with AST activity was assessed. Significant difference in post-thaw sperm motility was found between extender Androhep and Safecell Plus (P<0.05). AST activity did not differ significantly between tested extenders (P>0.05). In conclusion, the results of the study indicate that using Safecell Plus extender during holding period before cryopreservation significantly affected post-thaw sperm motility.


2020 ◽  
Vol 13 (5) ◽  
pp. 840-846
Author(s):  
R. Indriastuti ◽  
M. F. Ulum ◽  
R. I. Arifiantini ◽  
B. Purwantara

Aim: This study aimed to analyze the individual factors influencing the sperm quality of Bali bulls at Baturiti Artificial Insemination (AI) center. Materials and Methods: Semen that was ejaculated from nine Bali bulls was collected using artificial vaginas (n=5/bull). Semen ejaculates were evaluated immediately after collection to measure the quality of the fresh semen, including semen volume, sperm concentration, progressive motility, membrane integrity (MI), and abnormal morphology. Frozen semen was evaluated for progressive sperm motility, concentration, viability, MI, abnormal morphology, and deoxyribonucleic acid (DNA) fragmentation. Other secondary data, focusing on semen quantity (semen volume and sperm concentration), were also collected from frozen the semen production data of the Baturiti AI center from 2017 to 2019. Data were analyzed statistically using a completely randomized design, and one-way analysis of variance was applied to find differences among individual bulls. Results: Significant differences (p<0.05) were found among the bulls in semen volume, sperm motility, concentration, and MI of the fresh semen. Significant differences (p<0.05) were also found among the bulls in sperm motility, viability, MI, abnormal morphology, and DNA fragmentation of the frozen semen. Conclusion: Individual variation in all the tested sperm parameters of the fresh semen of Bali bulls, except sperm viability and abnormalities, was noted. Similarly, individual variation in all the tested sperm parameters in frozen semen, except sperm concentration, was noted. Therefore, individual factors can be used for selecting a superior bull in Bali cattle.


2014 ◽  
Vol 14 (1) ◽  
pp. 6-16
Author(s):  
Fitrah Khairi ◽  
Anis Muktiani ◽  
Yon Supri Ondho

(Effect of supplementation of vitamin E, Selenium and Zinc mineral against to nutrient consumption, production and quality cement simental cow)ABSTRACT. Simental bulls often experience a decline in production and semen quality in the rainy season, which have a higher humidity and high rainfall. This study aims to maintain nutrient consumption, production and semen quality Simental bulls during the rainy season through the supplementation of Vitamin E, Selenium and Zinc Minerals. The study was conducted in July-December 2012 at the Artificial Insemination Center (BIB) Unggaran. The research material used in this study were 12 males Simental cows BIB Unggaran grouped by age. The experimental design used in this study is a randomized block design (RBD) with 3 treatments T1 (ration + Vit. E), T2 (ration + Vit. E + Se), T3 (ration + Vit. E + Se + Zn) and 4 groups as replicates. Parameters measured were dry matter intake and nutrient consumption, production and semen quality. Cement production was measured from fresh semen volume during the study, whereas semen quality measured motility and concentration of spermatozoa from fresh semen. Data consumption of dry matter and nutrient intake obtained were processed using analysis of variance, followed by Duncan test. While data on production and semen quality were analyzed descriptively. The results showed that the combination of vitamin E supplementation, minerals Selenium and Zinc did not affect dry matter intake, total digestible nutriens and crude protein, but resulted in a decrease in the percentage of shelter, semen volume, sperm motility and concentration smaller than before treatment. Treatment T1, T2 and T3 resulted in a decrease in the number of shelter respectively 41,55%, 19,56% and 13,63% compared to before treatment, whereas a decrease in semen volume unchanged at 44,9 %, 43,7% and 40,99%. Sperm motility due to treatment T1, T2 and T3 respectively decreased by 55,87%, 22,10% and 13,63% compared to before treatment. In line with sperm motility, sperm concentration in treatment T , T2 and T3, respectively decreased by 49,16%, 22,85% and 14,88%. The conclusion is a combination of vitamin E supplementation, minerals Selenium and Zinc can prevent the decline in cement production, sperm motility and sperm concentration Simental bulls during the rainy season.


2019 ◽  
Vol 64 (No. 5) ◽  
pp. 209-216 ◽  
Author(s):  
R Ratchamak ◽  
T Vongpralub ◽  
W Boonkum ◽  
V Chankitisakul

The purpose of this study was to examine sperm quality after cryopreservation of ejaculates collected as a bulk sample, which is routinely part of semen collection, and to compare this quality with the sperm-rich fraction in boars. Ejaculates were collected as sperm-rich fractions (SRF) and bulk samples (BE) using a gloved-hand technique. Fresh semen quality in terms of semen volume, sperm concentration, total sperm motility and pH were conventionally evaluated. Then, semen was cryopreserved using the liquid nitrogen vapour method. The post-thaw sperm quality was evaluated by assessing sperm motility, live sperm with normal apical ridge and high mitochondrial energy status, lipid peroxidation was evaluated using CASA and fluorescent multiple staining and MDA levels were determined using a spectrophotometer, respectively. In terms of fresh semen quality, sperm motility in fresh semen did not differ significantly between the two groups. The treatment with the greater mean volume (BE; P &lt; 0.05) had a lower mean sperm concentration (P &lt; 0.05); meanwhile, the mean ejaculate pH collected as BE was more basic compared with SRF (P &lt; 0.05). However, there were no significant post-thaw quality changes between sperm-rich fractions and bulk samples of semen. In conclusion, ejaculates can be collected as bulk samples without the need to classify fractions for boar semen cryopreservation.


Author(s):  
A. Gopinathan ◽  
S.N. Sivaselvam ◽  
S.M.K. Karthickeyan ◽  
R. Venkataramanan ◽  
K. Kulasekar

The present study was aimed to investigate the effects of body weight and scrotal circumference on semen production traits of 136 crossbred Jersey bulls, which were maintained at three frozen semen stations of Tamil Nadu, India. Semen production traits such as semen volume, sperm concentration, mass activity, initial sperm motility, post-thaw motility and number of doses per ejaculate were taken for this study. The overall mean values for semen volume (ml), sperm concentration (millions per ml), mass activity (0 to 5 scale), initial sperm motility (per cent), postthaw motility (per cent) and number of doses per ejaculate were 4.18 ± 0.02, 1052.80 ± 7.26, 2.09 ± 0.07, 63.36 ± 0.00, 50.52 ± 0.00 and 215.50 ± 2.01 respectively. From this study, it revealed that the body weight and scrotal circumference of bulls significantly influenced all semen production traits except mass activity. Bulls having a body weight between 350 to 550 kg produced better quality semen and more number of frozen semen doses per ejaculate. When the scrotal circumference was >36 cm, majority of the semen production traits showed better values. The body weight was positively and significantly correlated with scrotal circumference, Semen volume and number of frozen semen doses produced per ejaculate. The scrotal circumference was positively and significantly correlated with semen volume, mass activity and number of doses per ejaculate; negatively and significantly correlated with initial sperm motiltiy.


2021 ◽  
Vol 5 (2) ◽  
Author(s):  
Arzu Yurci ◽  
Nur Dokuzeylul Gungor

Aim: To investigate the effect of increasing male age on the semen parameters including semen volume, sperm concentration, and progressive sperm motility. Materials and Methods: 1038 patients who applied to our IVF clinic for semen analysis or for infertility treatment were included in the study. The participants were divided into three age categories as follows: 680 participants (65.5%) aged 22-30, 139 participants (13.4%) aged 31-45 years, and 219 participants (21.1%) aged ≥45 years. Demographic and baseline clinical characteristics were recorded. The variables considered in this study were age and the following semen parameters: volume (ml); sperm concentration (millions/ml); and progressive sperm motility A+B (%). Normal semen values were defined based on the WHO criteria (World Health Organization, 2010). Results: The mean age of the participants was 33.1(± 4.8). The mean BMI of the participants was 24.1(± 2.8). The mean semen volume was 3.3 (± 1.5), the mean semen concentration was 43.6 (± 31.7), and the mean progressive sperm motility was 43.6 (± 10.1). There was a moderate negative correlation between paternal age and semen volume (r=-0.13) and sperm motility (r=-0.32), while there was a moderate positive correlation between paternal age and sperm concentration (r=0.24). The proportion of likelihood ratio measured between semen volume, sperm concentration, sperm motility and age was found to be 1 in patients between the ages of 22-30. The 22-30 age group does not have a decreasing or increasing effect on semen volume (OR: 1), sperm concentration (OR: 1) and motility (OR: 1). Regression analysis of patients in the 31-45 age group revealed no change in semen volume (OR: 0.99, 95% CI: 0.43-2.3, p <0.9), but an increase in sperm concentration (OR: 1.74, 95% CI: 1.13-2.7, p <0.01), and decreased sperm motility (OR: 0.28, 95% CI: 0.16-0.47, p <0.00). In the regression analysis of participants over the age of 45, semen volume (OR: 0.31, 95% CI: 0.19-0.53, p <0.00) and sperm motility (OR: 0.05, 95% CI: 0.03-0.08, p <0.00) decreased with age. No change was detected in the concentration (OR: 0.87, 95% CI: 0.63- 1.2, p <0.3). Conclusion: With increasing male age, semen volume and sperm motility begin to decrease. However, a significant decrease in sperm concentration does not occur until the age of 45.


2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
H Bahri ◽  
W Zidi ◽  
M Benkhalifa

Abstract Study question What is the relationship between Sperm DNA fragmentation (SDF) levels and sperm analysis (Spermocytogramme) parameters results? Summary answer SDF level of patients with pathological spermocytogramme presents negative correlations to total spermatozoa mobility, vitality and concentration, and positive correlation to sperm morphology defects. What is known already The relationship between SDF and Sperm analysis parameters and especially sperm morphology needs to be more studied since few studies over the last years were focused on this relationship. However, abnormalities in these two parameters are considered as the most important biological indicators of male infertility. The pathogenesis of Teratozoospermia (&lt;4% morphologically normal sperm cells according to WHO 2010) is continuously increasing over the last decade according to several studies. In addition, SDF is also increasing over the years because of several factors such as pollution, stress and lifestyle changing. Study design, size, duration Retrospective study including 331 infertile patients undergoing SDF-index testing with Spermocytogramme from January 2013 – December 2018. Patients divided into two groups: 143 patients with normal-Spermocytogramme and 188 patients with pathological-Spermocytogramme. Each group includes patients with abnormal SDF levels (&gt;30%). Statistical analyzes were performed using SPSS22.0 for Windows-software. Kolmogorov–Smirnov-test for normality analysis and comparisons by Student-t-test or Mann–Whitney U-test, as appropriate. Pearson/Spearman’ tests for correlations were used as appropriate, P-value&lt;0.05 was considered as significant. Participants/materials, setting, methods 143 patients with normal Spermocytogramme (2.8% abnormal-SDF) vs 188 patients with pathological Spermocytogramme (10.6% abnormal-SDF). WHO–2010 instructions for sperm-analysis were used through Makler®-counting-chamber (Sefi-Medical Instrument Ltd) for sperm-concentration and motility-determination using Sperm-class-analyzer-software (CASA-system (Microptic®)) to detect sperm abnormalities. Normozoospermia was determined when sperm progressive-motility is ≥ 32%, sperm-concentration ≥15x106/mL, and sperm-morphology ≥4%. “Diff-Quick” staining-method for the coloration of the fixed-sperm-slides was used for Sperm-morphology analysis. GoldCyto Sperm®Kit (Goldcyto Biotech corp.) was used to analyze SDF. Main results and the role of chance SDF is significantly higher in pathological spermocytogramme’ patients than in normal spermocytogramme’ patients (17.02 ± 11.88 vs 12.16 ± 9.58 respectively). In patients with pathological spermocytogramme, SDF is negatively correlated to Progressive sperm motility (r= –0.137; p = 0.042), Total sperm motility (r= –0.153; p = 0.036), vitality (r=–0.140; p = 0.048) and concentration (r=–0.195; p = 0.007). In the other hand, SDF presented positive correlation with teratozoospermia and especially with sperm midpiece defects (r = 0.171; p = 0.02). However, SDF did not present any correlation with age, testosterone levels and total ejaculated sperm volume. However the latter was positively correlated to spermatozoa midpiece and head defects (r = 0.156; p = 0.034; r = 0.203; p = 0.006, respectively). These results are in accordance with García-Ferreyra et al. (2014) who found that men with abnormal spermatozoa morphology showed high levels of DNA fragmentation, Sá et al. (2015) who confirmed that semen with lower concentration, motility and morphology have higher levels of SDF and showed that sperm head staining patterns are correlated with the degree of SDF. In addition, recently the study of Jakubik-Uljaszstudy et al. (2020) could confirms our results when it concluded that detailed sperm structural defects coexist with abnormal nuclear sperm DNA dispersion and that men with teratozoospermia may have a higher risk for sperm DNA damage. Limitations, reasons for caution Our study is a retrospective statistical investigation that included patients attending to the laboratory for fertility diagnosis after a period of infertility. Meta-analyzes studies in addition to more prospective-randomized-controlled-trials with couples undergoing assisted-reproductive-treatments and in comparison with fertile men are needed to confirm the relationship between SDF and spermocytogramme defects. Wider implications of the findings: These results should interest andrologists, reproductive science fundamentalists and embryologists who want to improve the investigations on the origin of infertility especially when it comes from male side. Trial registration number Not applicable


2015 ◽  
Vol 87 (3) ◽  
pp. 194 ◽  
Author(s):  
Mehmet Zeynel Keskin ◽  
Salih Budak ◽  
Tuǧba Zeyrek ◽  
Orçun Çelik ◽  
Oguz Mertoglu ◽  
...  

Objective: The aim of this study was to investigate the effect of serum gonadotropin and total testosterone levels on semen parameters. Materials and Methods: Three hundred and eighty-two patients that applied to a male infertility polyclinic were included in our study. Serum gonadotropin and total testosterone levels and semen parameters of the patients were analyzed during the first visit to the clinic. The reference FSH value was 1.5-12.4 mIU/mL, that of LH was 1.7-8.6 mIU/mL and the reference value for total testosterone was 249-836 ng/dL. Results: While there was no statistically significant difference between the patients with low gonadotropin levels and the controls regarding any of the semen parameters (p &gt; 0.05), there was a strong statistically significant difference between the patients with high gonadotropin levels and the controls regarding sperm concentration (p = 0.000), total motility (p = 0.000), progressive motility (p = 0.000), and morphology (p = 0.000). There was a strong statistically significant difference between the patients with low testosterone levels and the controls regarding total motility (p = 0.012) and progressive motility (p = 0.010), and a weak statistically significant difference in morphology (p = 0.042). There was no statistically significant difference in semen volume or sperm concentration (p &gt; 0.05). There was no statistically significant difference in any of the semen parameters between the patients with high testosterone levels and the controls (p &gt; 0.05). Conclusions: Our findings especially regarding LH and T levels are not in agreement with previous reports. In this regard, there is a need for larger-scale and randomized trials to resolve this discrepancy.


2021 ◽  
Vol 3 (1) ◽  
pp. 103-107
Author(s):  
Sadiq Abu ◽  
Hadijat O. Kolade-Yunusa ◽  
Terkaa Atim ◽  
F. Ehimatie Obakeye ◽  
Nuhu K. Dakum

Background: Infertility is a major cause of marital disharmony in Nigeria because of the high premium placed on childbearing. Unfortunately, the blame is on the woman most times in Nigeria. Seminiferous tubules comprise 80-90% of testicular mass. Thus, the testicular volume is an index of spermatogenesis. Therefore, accurate testicular volume will help in assessing testicular function if there is no obstruction. This study was conducted to evaluate the correlation between testicular volume, measured by ultrasound and conventional sperm parameters (semen volume, sperm concentration, sperm motility and sperm morphology) in men with infertility presenting to Urology Division, University of Abuja Teaching Hospital, Gwagwalada Abuja. Methodology: This was a descriptive cross sectional study, male subjects with infertility presenting to University of Abuja Teaching Hospital were recruited into the study. The testicular volume of all the subjects was measured by ultrasonography. The semen samples were collected by the process of masturbation on day five of sexual abstinence and analyzed according to WHO criteria 2010. Collated data were analyzed using SPSS version 20.0. P-value<0.05 was taken as statistically significant. Results: The average total testicular volume (TTV) of men with infertility was 19.83 ml while mean testicular volume (MTV) was 10.24 ml. There was a statistically significant difference between MTV and semen volume (r=0.391, p=0.000), and sperm concentration (r=0.639, p=0.000). There was a weak and insignificant correlation between MTV and sperm motility and MTV and morphology (r=0.216, p=0.055 and r=0.076, p=0.502) respectively. Linear regression analysis showed significant impact of MTV (P<0.001) on sperm concentration with a critical MTV of ≤10ml.0 indicating poor testicular function (severe oligospermia) Conclusion: The mean ultrasonic testicular volume in men with infertility in this study was 10.24ml and there was a significant positive correlation of MTV with semen volume and sperm concentration. Although, there was correlation of MTV with sperm motility and morphology, this was not significant. The critical mean ultrasonic testicular volume indicating poor testicular function (severe oligospermia) was found to be 10ml. This study has shown that the mean ultrasonic testicular volume can be a pointer to semen volume and sperm concentration in evaluating men with infertility.


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