Biomass production of fungus \(\textit{Termitomyces clypeatus}\) in a stirred-tank bioreactor

Termitomyces clypeatus is a fungus species, which has been used as food and medicinal mushroom. In the current study, submersed fermentation of the fungus in a stirred-tank bioreactor was determined for its culture biomass and nutrient contents. T. clypeatus obtained its highest biomass after from 13 to 15 days of cultivation, when the dry biomass of its mycelium system was more than 6%. Agitation speeds of 150 or 180 rpm were the most suitable for the fungus. The determination of chemical compositions showed that the dry biomass of its mycelium system had high protein content, with 57.8% on its dry-weight basis. Besides, 11 types of amino acids higher than 0.1% of wet weight culture were found in fermentation products. Moreover, dried mycelia of the fungus contained 27.4% carbohydrates. The protein and carbohydrate containing in the mycelium and fruit body of T. clypeatus were not statistically different. These results showed that a stirred-tank bioreactor could be applied to culture the fungus.

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PHENOLIC COMPOUNDS OF GREAT BURNET SANQUISORBA OFFICINALIS (L.) CELL CULTURE

chemistry of plant raw material ◽

10.14258/jcprm.2019014119 ◽

2019 ◽

pp. 241-250

Author(s):

Антон (Anton) Николаевич (Nikolaevich) Акулов (Akulov)

Keyword(s):

Phenolic Compounds ◽

Suspension Culture ◽

Condensed Tannins ◽

Hplc Analysis ◽

Dry Weight ◽

Callus Cultures ◽

Biologically Active ◽

Potential Source ◽

Dry Biomass ◽

Wet Weight

Callus cultures were obtained from the seeds of great burnet plants. Long-passed suspension cultures were obtained by transferring callus cultures to a liquid medium. The increase in the biomass of the suspension culture for the passage was 501%, the yield of dry biomass was 120 mg/g wet weight. The content of methanol-extracted phenolic compounds in the suspension culture increased during the passage and reached a maximum (34.6 mg/g dry weight) on the 22nd day of cultivation. An HPLC analysis of the phenolic compounds of the suspension culture and the rhizomes of the herbaceous plants was carried out. The proportion of some phenolic compounds in the obtained culture is significantly higher compared to their proportion in the rhizomes of plants. Such compounds were gallic, ferulic and caffeic acids, as well as condensed tannins. The obtained culture can be an object for conducting experiments on enhancing the synthesis of phenolic using various inductors. All this allows us to consider the obtained culture as a potential source of biologically active phenolic compounds.

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BIOASSAY OF PROLACTIN

Acta Endocrinologica ◽

10.1530/acta.0.0600091 ◽

1969 ◽

Vol 60 (1) ◽

pp. 91-100 ◽

Cited By ~ 5

Author(s):

Charles S. Nicoll

Keyword(s):

Epithelial Cells ◽

Lipid Content ◽

Dry Weight ◽

Fat Content ◽

Assay Sensitivity ◽

Mucosal Epithelium ◽

Wet Weight ◽

Total Dry Weight

ABSTRACT The response of the pigeon crop-sac to systemically acting prolactin (injected subcutaneously) was evaluated by measuring the wet weight of the responsive lateral lobes of the organ and by determining the dry weight of a 4 cm diameter disc of mucosal epithelium taken from one hemicrop. Of several different injection schedules tested, administration of prolactin in four daily injections was found to yield optimal responses. When compared with a graded series of prolactin doses, measurement of the mucosal dry weight proved to be a better method of response quantification than determination of the crop-sac wet weight with respect to both assay sensitivity and precision. The submucosal tissue of the crop-sac was estimated to constitute about 64 % of the total dry weight of the unstimulated organ and it was found to be relatively unresponsive to prolactin stimulation in comparison with the mucosa. The lipid content of the mucosal epithelium was determined using unstimulated crop-sacs or tissues which showed varying degrees of prolactin-induced proliferation. The fat content of the mucosal epithelial cells increased only slightly more rapidly than the dry weight or the defatted dry weight of the mucosa. Suggestions are made for the further improvement of the systemic crop-sac assay for prolactin.

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Effect of rhizobacteria strains on the induction of resistance in barley genotypes against Cochliobolus sativus

Hellenic Plant Protection Journal ◽

10.2478/hppj-2020-0009 ◽

2020 ◽

Vol 13 (2) ◽

pp. 83-92 ◽

Cited By ~ 1

Author(s):

A. Adam

Keyword(s):

Plant Growth ◽

Pseudomonas Putida ◽

Growth Parameters ◽

Disease Incidence ◽

Dry Weight ◽

Cochliobolus Sativus ◽

Resistance Level ◽

Wet Weight ◽

Number Of Leaves ◽

Barley Genotypes

SummaryEnhancement of the resistance level in plants by rhizobacteria has been proven in several pathosystems. This study investigated the ability of four rhizobacteria strains (Pseudomonas putida BTP1 and Bacillus subtilis Bs2500, Bs2504 and Bs2508) to promote the growth in three barley genotypes and protect them against Cochliobolus sativus. Our results demonstrated that all tested rhizobacteria strains had a protective effect on barley genotypes Arabi Abiad, Banteng and WI2291. However, P. putida BTP1 and B. subtilis Bs2508 strains were the most effective as they reduced disease incidence by 53 and 38% (mean effect), respectively. On the other hand, there were significant differences among the rhizobacteria-treated genotypes on plant growth parameters, such as wet weight, dry weight, plant height and number of leaves. Pseudomonas putida BTP1 strain was the most effective as it significantly increased plant growth by 15-32%. In addition, the susceptible genotypes Arabi Abiad and WI2291 were the most responsive to rhizobacteria. This means that these genotypes have a high potential for increase of their resistance against the pathogen and enhancement of plant growth after the application of rhizobacteria. Consequently, barley seed treatment with the tested rhizobacteria could be considered as an effective biocontrol method against C. sativus.

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Respon Akar Bibit Aren Genjah (Arenga pinnata) Di Pembibitan Pada Pemberian Dosis dan Interval Pupuk Organik Cair Nasa

Agrifarm : Jurnal Ilmu Pertanian ◽

10.24903/ajip.v7i1.368 ◽

2018 ◽

Vol 7 (1) ◽

pp. 28

Author(s):

Yetti Elidar

Keyword(s):

Organic Fertilizer ◽

Dry Weight ◽

Treatment Combination ◽

Root Dry Weight ◽

Randomized Design ◽

Dose Study ◽

Palm Sugar ◽

Wet Weight ◽

Completely Randomized Design ◽

Leaf Dry Weight

Research on the response of roots of palm sugar palm seeds (Arenga pinnata) in nurseries at doses and intervals of Nasa liquid organic fertilizer. Aims to determine the dosage, interval and combination of dosages and fertilization intervals with Nasa liquid organic fertilizer which can provide the best dry weight of the roots in the nursery. The research design used was a Completely Randomized Design (CRD) with 3x3 factorial experiments and each treatment was repeated 8 (eight) times, consisting of: the first factor was the treatment of POC Nasa dose in a concentration of 3 cc POC Nasa per liter of water (D) consists of 3 levels, namely: d1 = 300 ml POC Nasa, d2 = 400 ml POC Nasa, d3 = 500 ml POC Nasa, while the second factor is the treatment of POC Nasa Interval (I) consisting of 3 levels, namely: i1 = 2 once a week, i2 = once every 3 weeks, i3 = once every 4 weeks. The results of the POC Nasa dose study had a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level are: d2 (400 ml of Nasa liquid organic fertilizer), the interval of liquid organic fertilizer Nasa has a significant effect on leaf wet weight, leaf dry weight, root wet weight and root dry weight. The best dose at this level is: i1 (once every 2 weeks). The treatment combination has no significant effect on all parameters.

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PHYTOTOXICITY OF WASTEWATER CONTAINING CADMIUM (Cd) USING OLEANDER

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v51i4.1103 ◽

2020 ◽

Vol 51 (4) ◽

pp. 1231-1238

Author(s):

Zeki & Ridha

Keyword(s):

Translocation Factor ◽

Preliminary Test ◽

Dry Weight ◽

Exposure Dose ◽

Test Duration ◽

Plant Analysis ◽

Wet Weight ◽

Simulated Wastewater ◽

Almost All ◽

Plant Shoots

This study was aimed to investigate the ability of N.oleander to remove Cadmium (Cd) from wastewater. A prolonged toxicity test was performed in a single exposure and run for 65 days with various concentrations of Cd. Plants were grown in sand medium and irrigated with simulated wastewater contaminated with Cd, using different concentrations (0, 10, 25, 50, 75 mg/L), which were chosen based on previous preliminary test. The results of physical observation of the plants didn’t show any withering symptoms. The Cd concentration in plants increased, while in water decreased. The results of plant analysis showed that Cd concentration in plant shoots (stems and leaves) was higher than that in roots for almost all exposure doses along the test duration. The concentration of Cd in water decreased significantly from the first week of the test and become (0 mg/l) on day-35 for 10 and 25 mg/l exposure doses, while exceeded the permissible limits for 50 and 75 mg/l exposure doses and were 0.14 and 0.91 mg/l, respectively. Wet weight and dry weight of Oleander decreased with increasing Cd concentration level except for 10 mg/l exposure dose where the plant wet weight and dry weight increased at the end of the test. Bioaccumulation factor (BAF) and Translocation Factor (TF) was found to be greater than 1, indicating that Oleander is a successful hyperaccumulator for Cd.

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Dynamical simulation of a continuous stirred tank bioreactor with the use of cellular automata for the biofilm description

Chemical Engineering Research and Design ◽

10.1016/j.cherd.2021.02.019 ◽

2021 ◽

Vol 168 ◽

pp. 264-274

Author(s):

Szymon Skoneczny ◽

Monika Cioch-Skoneczny

Keyword(s):

Cellular Automata ◽

Stirred Tank ◽

Stirred Tank Bioreactor ◽

Dynamical Simulation ◽

Continuous Stirred Tank

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High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources

Process Biochemistry ◽

10.1016/j.procbio.2018.03.005 ◽

2018 ◽

Vol 69 ◽

pp. 1-11 ◽

Cited By ~ 15

Author(s):

Willian Daniel Hahn Schneider ◽

Roselei Claudete Fontana ◽

Simone Mendonça ◽

Félix Gonçalves de Siqueira ◽

Aldo José Pinheiro Dillon ◽

...

Keyword(s):

Nitrogen Sources ◽

White Rot ◽

Stirred Tank ◽

Carbon And Nitrogen Sources ◽

Stirred Tank Bioreactor ◽

Carbon And Nitrogen ◽

High Level ◽

Level Production

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Utilisation of winery wastewater for xanthan production in stirred tank bioreactor: Bioprocess modelling and optimisation

Food and Bioproducts Processing ◽

10.1016/j.fbp.2019.06.019 ◽

2019 ◽

Vol 117 ◽

pp. 113-125 ◽

Cited By ~ 3

Author(s):

Zorana Rončević ◽

Jovana Grahovac ◽

Siniša Dodić ◽

Damjan Vučurović ◽

Jelena Dodić

Keyword(s):

Stirred Tank ◽

Stirred Tank Bioreactor ◽

Winery Wastewater ◽

Xanthan Production

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Production of Newcastle Disease Virus by Vero Cells Grown on Cytodex 1 Microcarriers in a 2-Litre Stirred Tank Bioreactor

Journal of Biomedicine and Biotechnology ◽

10.1155/2010/586363 ◽

2010 ◽

Vol 2010 ◽

pp. 1-7 ◽

Cited By ~ 15

Author(s):

Mohd Azmir Arifin ◽

Maizirwan Mel ◽

Mohamed Ismail Abdul Karim ◽

Aini Ideris

Keyword(s):

Cell Culture ◽

Newcastle Disease Virus ◽

Disease Virus ◽

Newcastle Disease ◽

High Speed ◽

Virus Titer ◽

Vero Cells ◽

Stirred Tank ◽

Stirred Tank Bioreactor ◽

Maximum Cell

The aim of this study is to prepare a model for the production of Newcastle disease virus (NDV) lentogenic F strain using cell culture in bioreactor for live attenuated vaccine preparation. In this study, firstly we investigated the growth of Vero cells in several culture media. The maximum cell number was yielded by culture of Vero cells in Dulbecco's Modified Eagle Medium (DMEM) which was1.93×106 cells/ml. Secondly Vero cells were grown in two-litre stirred tank bioreactor by using several commercial microcarriers. We achieved the maximum cell concentration about7.95×105 cells/ml when using Cytodex 1. Later we produced Newcastle Disease virus in stirred tank bioreactor based on the design developed using Taguchi L4 method. Results reveal that higher multiplicity of infection (MOI) and size of cell inoculums can yield higher virus titer. Finally, virus samples were purified using high-speed centrifugation based on3∗∗(3-1) Fractional Factorial Design. Statistical analysis showed that the maximum virus titer can be achieved at virus sample concentration of 58.45% (v/v), centrifugation speed of 13729 rpm, and centrifugation time of 4 hours. As a conclusion, high yield of virus titer could be achieved through optimization of cell culture in bioreactor and separation by high-speed centrifugation.

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