scholarly journals Evaluation of Betulin and Betulinic Acid Content in Birch Bark from Different Forestry Areas of Western Carpathians

2012 ◽  
Vol 40 (2) ◽  
pp. 99 ◽  
Author(s):  
Liviu HOLONEC ◽  
Floricuta RANGA ◽  
Diana CRAINIC ◽  
Alina TRUTA ◽  
Carmen SOCACIU
Keyword(s):  
Betulinic Acid ◽  
Acid Content ◽  
High Performance ◽  
Uv Detection ◽  
Western Carpathians ◽  
Tree Bark ◽  
Birch Bark ◽  
Birch Tree ◽  
Birch Trees ◽  
Absolute Concentrations

In order to evaluate the content of betulin and betulinic acids in Birch barks originating from Western Carpathians, Transylvania (Romania) forests, in relation with the location of birch trees, it was recorded the geographic and vegetation conditions at the specific locations of each type of sample. Two groups of birch tree bark (totally 10 samples) were collected, from two regions, pasture surfaces where birch trees appeared spontaneously (samples 1 to 5) and another group from forests of combined trees, including mainly pine trees (samples 6 to 10). The identification and quantitation of betulin and betulinic acid were made by High Performance Liquid Chromatography with UV detection. In the first region it has been found mean concentrations of 126.85 ± 12.56 mg/g betulin bark while acid betulinic had average values of 12.78 ± 1.26 mg/g bark. In the second region we found mean concentrations of 89.84 ± 8.43 mg/g betulin of bark while acid betulinic had average values of 10.60 ± 0.97 mg/g bark. Statistically, significant differences were noticed between the two regions, the first region being richer in these molecules. Generally, the percentage of betulinic acid was around 11.23% against betulin, higher in the second region, even the absolute concentrations were lower for both molecules. These data are useful indicators of the potential offered by birch bark sources found in Transylvania region, to obtain extracts enriched in betulin and betulinic acid.

The Study of the Kinetics of Obtaining Betulin and Extractive Substances from the Birch Tree Bark

2017 ◽  
Vol 29 ◽  
pp. 42-47
Author(s):  
Sergei I. Tretiakov ◽  
Elena N. Koptelova ◽  
Natalia A. Kutakova ◽  
Nina V. Bagretsova ◽  
Nadezhda L. Voropaeva
Keyword(s):  
Tree Bark ◽  
Birch Bark ◽  
Hydrodynamic Conditions ◽  
Food Industries ◽  
Plywood Industry ◽  
Birch Tree ◽  
Extractive Substances ◽  
Bioactive Agents ◽  
The Given ◽  
Kinetics Of

Birch bark attracts attention of researchers as a source of betulin and other extractive substances (ES) useful for chemical, pharmaceutical and food industries. A challenge is to develop an efficient way of obtaining these bioactive agents. This paper presents a microwave-assisted technique of extracting these substances from the waste birch bark of the plywood industry. The kinetics of the extraction was investigated. With the process going on in the pore-diffusion area, the hydrodynamic conditions of the operation are discussed. An algorithm for calculating the kinetics of the procedure was developed and experimentally confirmed. The estimations for the process were made at constant values of the internal diffusion coefficient. The value of the coefficient was found to be a function of the birch bark fragments dimensions. An equation was established to estimate the duration of the extraction to obtain the given yield of ES and betulin.

scholarly journals Anti-Inflammatory and Anticancer Properties of Birch Bark-Derived Betulin: Recent Developments

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2663
Author(s):  
Hardeep Singh Tuli ◽  
Katrin Sak ◽  
Dhruv Sanjay Gupta ◽  
Ginpreet Kaur ◽  
Diwakar Aggarwal ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Tree Bark ◽  
Antitumor Action ◽  
Special Focus ◽  
Birch Bark ◽  
Related Factor ◽  
Nuclear Factor ◽  
Anticancer Activities ◽  
Birch Tree ◽  
Anti Inflammatory

Birch tree bark-derived betulin has attracted scientific interest already for several centuries, being one of the first natural products identified from plants. However, the cellular events regulated by betulin and precise molecular mechanisms under these processes have been begun to be understood only recently. Today, we know that betulin can exert important anticancer activities through modulation of diverse cellular pathways. In this review article, betulin-regulated molecular signaling is unraveled and presented with a special focus on its participation in anti-inflammatory processes, especially by modulating nuclear factor-κB (NF-κB), prostaglandin/COX, and nuclear factor erythroid2-related factor 2 (Nrf2)-mediated cascades. By regulating these diverse pathways, betulin can not only affect the development and progression of different cancers, but also enhance the antitumor action of traditional therapeutic modalities. It is expected that by overcoming the low bioavailability of betulin by encapsulating it into nanocarriers, this promising natural compound may provide novel possibilities for targeting inflammation-related cancers.

Identification and isolation of betulin, betulinic acid and lupeol from birch bark

Planta Medica ◽  
2009 ◽  
Vol 75 (09) ◽  
Author(s):  
E Kovac-Besovic ◽  
K Duric ◽  
Z Kaloðera ◽  
E Sofic
Keyword(s):  
Betulinic Acid ◽  
Birch Bark

Complexation with Hydroxypropyl-gama-Cyclodextrin of Birch Tree Extract Physico-chemical Characterisation of their Binary Products

2008 ◽  
Vol 59 (6) ◽  
Author(s):  
Codruta Soica ◽  
Cristina A. Dehelean ◽  
Valentin Ordodi ◽  
Diana Antal ◽  
Vicentiu Vlaia
Keyword(s):  
Inclusion Complexes ◽  
Water Solubility ◽  
In Vitro Dissolution ◽  
Molar Ratio ◽  
Bark Extract ◽  
Birch Bark ◽  
Preparation Methods ◽  
Birch Tree ◽  
Physico Chemical

Birch bark contains important pentacyclic triterpens that determine an anticancer, anti-inflammatory and antiviral activity. The compounds can be extracted by simple procedures with organic solvents. The major problem of this type of triterpens is their low water solubility which can be increased by physical procedures like cyclodextrin complexation. The aim of present study was to analyse the products between birch bark extract and hydroxypropyl-g -cyclodextrin. Hydroxypropyl-g -cyclodextrin (HPGCD) was used as a host to improve its solubility in water, via inclusion complex formation. In order to obtain the inclusion complexes, 1:2 molar ratio and two preparation methods (physical mixing, kneading) were used. The inclusion complexes were analyzed by in vitro dissolution tests, thermal analysis and X-ray diffraction.

Anti-inflammatory Effects ofScoparia dulcisL. and Betulinic Acid

2011 ◽  
Vol 39 (05) ◽  
pp. 943-956 ◽  
Author(s):  
Jen-Chieh Tsai ◽  
Wen-Huang Peng ◽  
Tai-Hui Chiu ◽  
Shang-Chih Lai ◽  
Chao-Ying Lee
Keyword(s):  
Betulinic Acid ◽  
High Performance ◽  
Ethanol Extract ◽  
Paw Edema ◽  
Scoparia Dulcis ◽  
Inflammatory Mechanism ◽  
Tnf Α ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Analytical Result

The aims of this study intended to investigate the anti-inflammatory activity of the 70% ethanol extract from Scoparia dulcis (SDE) and betulinic acid on λ-carrageenan-induced paw edema in mice. The anti-inflammatory mechanism of SDE and betulinic acid was examined by detecting the levels of cyclooxygenase-2 (COX-2), nitric oxide (NO), tumor necrosis factor (TNF-α), interleukin-1β (IL-1β) and malondialdehyde (MDA) in the edema paw tissue and the activities of superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GRd) in the liver. The betulinic acid content in SDE was detected by high performance liquid chromatography (HPLC). In the anti-inflammatory model, the results showed that SDE (0.5 and 1.0 g/kg) and betulinic acid (20 and 40 mg/kg) reduced the paw edema at 3, 4 and 5 h after λ-carrageenan administration. Moreover, SDE and betulinic acid affected the levels of COX-2, NO, TNF-α and IL1-β in the λ-carrageenan-induced edema paws. The activities of SOD, GPx and GRd in the liver tissue were increased and the MDA levels in the edema paws were decreased. It is suggested that SDE and betulinic acid possessed anti-inflammatory activities and the anti-inflammatory mechanisms appear to be related to the reduction of the levels of COX-2, NO, TNF-α and IL1-β in inflamed tissues, as well as the inhibition of MDA level via increasing the activities of SOD, GPx and GRd. The analytical result showed that the content of betulinic acid in SDE was 6.25 mg/g extract.

Determination of ascorbic acid in the retina during chicken embryo development using high performance liquid chromatography and UV detection

2016 ◽  
Vol 8 (27) ◽  
pp. 5441-5447 ◽  
Author(s):  
Débora R. S. Lima ◽  
Marcelo Cossenza ◽  
Carlos Gustavo Garcia ◽  
Camila C. Portugal ◽  
Flávia F. de C. Marques ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Embryo Development ◽  
Chicken Embryo ◽  
High Performance ◽  
Uv Detection

A HPLC-UV method has been developed and validated for the determination of ascorbic acid in chicken embryo retina.

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