scholarly journals Comparative study of some physicochemical and biological properties of effect host species variation on the relationship Saharan parasitic plant Cistanche violaceae (Desf.) Beck.

2021 ◽  
Vol 13 (4) ◽  
pp. 11054
Author(s):  
Fatma ALIA ◽  
Atef CHOUIKH ◽  
Ali Boutlelis DJAHRA ◽  
Aida BOUSBIA BRAHIM ◽  
Sadok NANI ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Host Species ◽  
Reducing Power ◽  
Total Content ◽  
Parasitic Plants ◽  
Parasitic Plant ◽  
Biological Properties ◽  
Species Variation ◽  
Two Samples ◽  
Physicochemical And Biological Properties

This work aims to study the effect of different host species on physicochemical and biological properties of the Saharan parasitic plant Cistanche violacea that grows parasitized on two hosts Haloxylon articulatum and a Limonistrum guyonianum in the eastern desert of Algeria. The physicochemical characteristics of C. violcea showed an affinity for the results of both ash and pH and it showed a difference in the amount content of carbohydrates and the value of electrical conductivity. For the content of polyphenols, flavonoids, anthocyanins and tannins the highest values were recorded in C. violcea, which was parasitized on H. articulatum. While, the results of HPLC have identified nine compounds in the crude extracts of the parasitic plants and their hosts in different concentrations. In antioxidant activity, the tannin and anthocyanin extracts from C. violcea parasitized on H. articulatum showed better inhibition of DPPH• radical and best the total antioxidant capacity respectively, but the tannins extract of C. violcea parasitized on L. guyonianum given best reducing power capacity. In SPF assay by UV-Vis spectrophotometry method, all extracts of the parasitic plants showed mild to moderate sun protection. Statically the host variation did not show any significant differences in the physicochemical analysis and the quantitative and qualitative total content of polyphenols. While the significant differences appeared through the antioxidant activity tests, especially between C. violcea and its host H. articulatum, between C. violacea and its host H. articulatum and between the two samples of parasitic plants.

scholarly journals Determination of in vitro antioxidant activity of the leaves extracts of Ehretia pubescens

2020 ◽  
Vol 11 (4) ◽  
pp. 6262-6267
Author(s):  
Krishnamoorthy Meenakumari ◽  
Giridharan Bupesh ◽  
Mayur Mausoom Phukan
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Biological Properties ◽  
Scavenging Activity ◽  
Reducing Power Assay

The foods from plants were known to ensure against degenerative diseases and maturing because of their antioxidant activitycredited to their high content. Information on antioxidant activity of Indian medicinal plant is abundant. To the best of our knowledge, biological properties have not been accounted in the literature for this species of . As a point, this is the first results to assess the anti-oxidant activity of the plant which belongs to the family . The antioxidant activity of Methanol, , Ethyl acetate and Aqueous extracts of E. was determined using the DPPH free radical scavenging activity, ABTS radical scavenging activity and reducing power assay. The DPPH scavenging activity showed higher activity observed in extract (63%) of E. than (54%), (44%) and aqueous (30%). the ABTS assay inhibition in extract (58%) than (43%), (38%) and aqueous (32%) extracts. The reducing power assay of different extracts was increased in extract (54%) than (40%), (34%) and aqueous (28%) extracts. Overall, the and ethyl acetate extract had higher antioxidant properties than other extract. However, in this study, extracts exhibit great potential for antioxidant activity and may be useful for their nutritional and medicinal functions.

Evaluation of Antioxidant Activity of Some Common Mosses

2008 ◽  
Vol 63 (7-8) ◽  
pp. 476-482 ◽  
Author(s):  
Vladimír Chobot ◽  
Lenka Kubicová ◽  
Samar Nabbout ◽  
Ludek Jahodář ◽  
Franz Hadacek
Keyword(s):  
Antioxidant Activity ◽  
Cyclic Voltammetry ◽  
Iron Chelation ◽  
Reducing Power ◽  
Total Content ◽  
Positive Control ◽  
Type Reaction ◽  
Ethanol Extracts ◽  
Ribose Degradation ◽  
Antioxidant Effects

The antioxidant activity of ethanol extracts of Atrichum undulatum, Polytrichum formosum (Polytrichaceae), Pleurozium schreberi (Entodontaceae) and Thuidium tamariscinum (Thuidiaceae) was evaluated by an electrochemical method (cyclic voltammetry) and standard photometric methods: Fe(III) to Fe(II) reducing power, nitric oxide scavenging (NO) assay and simulation of Fenton-type reaction by nonsite-specific (NSSOH) and site-specific (SSOH) hydroxyl radical-mediated 2-deoxy-d-ribose degradation inhibition. The total content of phenols was determined by the Folin-Ciocalteau reagent. All tested species showed antioxidant effects lower than the positive control, caffeic acid. The extracts of A. undulatum and P. formosum contained the highest content of phenols and were the most effective in Fe(III) to Fe(II) reducing power, cyclic voltammetry and SSOH assay. By contrast, only the extract of Pl. schreberi showed activity in the NSSOH assay. A. undulatum and T. tamariscinum extracts were the most active in the NO assay. The results suggest that the extracts of A. undulatum and P. formosum possess stronger antioxidant activity than those of Pl. scheberi and T. tamariscinum, but they affect the Fenton-type reaction mainly by iron chelation.

scholarly journals Andean Prumnopitys Andina (Podocarpacae) Fruit Extracts: Characterization of Secondary Metabolites and Potential Cytoprotective Effect

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4028 ◽  
Author(s):  
Felipe Jiménez-Aspee ◽  
Cristina Theoduloz ◽  
Lisa Pormetter ◽  
Judith Mettke ◽  
Felipe Ávila ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
High Performance ◽  
Reducing Power ◽  
Biological Properties ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Array Detector ◽  
Ags Cells ◽  
Cytoprotective Activity ◽  
Trolox Equivalent

The fruits from the Chilean Podocarpaceae Prumnopitys andina have been consumed since pre-Hispanic times. Little is known about the composition and biological properties of this fruit. The aim of this work was to identify the secondary metabolites of the edible part of P. andina fruits and to assess their antioxidant activity by means of chemical and cell-based assays. Methanol extracts from P. andina fruits were fractionated on a XAD7 resin and the main compounds were isolated by chromatographic means. Antioxidant activity was determined by means of 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), ferric reducing power (FRAP), trolox equivalent antioxidant capacity (TEAC) and oxygen radical absorbance capacity (ORAC) assays. The cytoprotective activity of the extract against oxidative and dicarbonyl stress was evaluated in human gastric epithelial cells (AGS). The total intracellular antioxidant activity (TAA) of the extract was determined in AGS cells. The inhibition of meat lipoperoxidation was evaluated under simulated gastric digestion conditions. Rutin, caffeic acid β-glucoside and 20-hydroxyecdysone were identified as major components of the fruit extract. Additional compounds were identified by high-performance liquid chromatography diode-array detector mass spectrometry (HPLC-DAD-MSn) and/or co-injection with standards. Extracts showed dose-dependent cytoprotective effects against oxidative and dicarbonyl-induced damage in AGS cells. The TAA increased with the pre-incubation of AGS cells with the extract. This is the first report on the composition and biological activity of this Andean fruit.

scholarly journals The influence of gamma-irradiation on the formation of free radicals and antioxidant status of oregano (Origanum vulgare L.)

2008 ◽  
Vol 25 (No. 3) ◽  
pp. 131-143 ◽  
Author(s):  
J. Horváthová ◽  
M. Suhaj ◽  
M. Polovka ◽  
V. Brezová ◽  
P. Šimko
Keyword(s):  
Thermal Stability ◽  
Antioxidant Activity ◽  
Phenolic Compounds ◽  
Epr Spectroscopy ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Total Content ◽  
Life Time ◽  
Total Phenolic ◽  
Origanum Vulgare

The influence of various gamma-radiation dose absorptions on oregano (<i>Origanum vulgare</i> L.) solid samples was monitored by means of electron paramagnetic resonance (EPR) spectroscopy. Further, the antioxidant activity of oregano methanol/water extracts was characterised using DPPH (1,1-diphenyl-2-picrylhydrazyl free radical); thiobarbituric acid reactive substances (TBARS); ferric reducing power (FRP); and total content of phenolic compounds (TPC) assays. EPR spectroscopy was used for the investigation of the influence of the absorbed dose on the character of the paramagnetic structures formed, as well as for their thermal stability and life-time characterisation. EPR spectrum of the reference (non-irradiated) sample represents a broad singlet line with unresolved hyperfine splitting, attributable to Mn(II) ions, upon which the additional sharp EPR signal (g = 2.0022, &Delta;B<sub>pp</sub> &asymp; 1 mT) is superimposed, assigned to stable semiquinone radicals produced by the oxidation of polyphenolic compounds present in plants. The additional paramagnetic structures of different origin (mostly of cellulose and carbohydrate), possessing diverse thermal stability and life-time, were identified in the gamma-irradiated samples. Immediately after irradiation, a statistically significant increase was observed of the TBARS values and the total content of phenolic compounds in methanol/water oregano extract. The alterations of the antioxidant properties of oregano extracts with the time after the radiation treatment were also monitored. A substantial time-dependent decrease of antioxidant activity was observed, probably as a result of storage, with both irradiated and non-irradiated oregano samples, as obvious from the ferric reducing power test and the content of total phenolic substances. The influence of irradiation and subsequent storage of oregano samples on the DPPH radical-scavenging ability was only negligible.

scholarly journals Polyphenol components and antioxidant activity of Thymus spp.

10.5219/1715 ◽  
2022 ◽  
Vol 16 ◽  
pp. 1-14
Author(s):  
Olena Vergun ◽  
Liudmyla Svidenko ◽  
Olga Grygorieva ◽  
Vladimíra Horčinová Sedláčková ◽  
Katarína Fatrcová Šramková ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Reducing Power ◽  
Total Content ◽  
Scientific Work ◽  
Aromatic Plants ◽  
Dpph Method ◽  
Pharmacological Studies ◽  
Antioxidant Parameters ◽  
Trolox Equivalent ◽  
Gallic Acid Equivalent

  This scientific work was aimed to evaluate the antioxidant potential of aromatic plants of Thymus spp. in the East of Ukraine. These plants are known as medicinal and food around the world. All antioxidant parameters were investigated spectrophotometrically: total content of polyphenols (TPC), the total content of phenolic acids (TPAC), the total content of flavonoids (TFC), molybdenum reducing power of extracts (MRP), and antioxidant activity by DPPH method (DPPH). Investigation of ethanolic extracts demonstrated that TPC varied from 57.89 to 123.67 mg/g gallic acid equivalent (GAE) DW for Th. pulegioides, from 61.43 to 168.18 mg GAE/g for Th. serpyllum, and from 47.36 to 115.67 mg GAE/g for Th. vulgaris. TPAC ranged from 27.36 to 50.22 mg/g caffeic acid equivalent (CAE) DW for Th. pulegioides, from 28.58 to 59.62 mg CAE/g for Th. serpyllum, and from 22.95 to 53.82 mg CAE/g for Th. vulgaris. TFC was determined in a range from 29.88 to 61.23 mg/g quercetin equivalent (QE) DW for Th. pulegioides, from 36.0 to 82.43 mg QE/g for Th. serpyllum, and from 24.59 to 55.41 mg QE/g for Th. vulgaris. MRP was detected in the range of 94.65 – 204.76 mg/g Trolox equivalent (TE) DW for Th. pulegioides, 96.06 – 219.0 mg TE/g for Th. serpyllum, and 87.56 – 215.43 mg TE/g for Th. vulgaris. The antioxidant activity of extracts by the DPPH method was 6.34 – 9.23 mg TE/g for Th. pulegioides, 8.11 – 9.21 mg TE/g for Th. serpyllum, and 4.97 – 9.53 mg TE/g for Th. vulgaris. It was established that polyphenol accumulation depended on the growth stage and species. For all species was found a strong correlation between TPC and TFC (r = 0.938, 0.908, and 0.854). Investigated Thymus spp. are a valuable source of antioxidants that can be used in pharmacological studies and the food industry.

scholarly journals CHEMICAL COMPOSITION, ANTIOXIDANT ACTIVITY, AND FATTY ACID PROFILE OF COCOA NIBS

2021 ◽  
Vol 9 (6) ◽  
pp. 168-176
Author(s):  
Fernanda Côrrea Leal Penido ◽  
Isabelle Cristine Ramos Lourenço ◽  
Lívia Mariana da Silva ◽  
Cleverson Fernando Garcia ◽  
Flávia Augusta Guilherme Gonçalves Rezende
Keyword(s):  
Antioxidant Activity ◽  
Fatty Acid ◽  
Chemical Composition ◽  
Fatty Acid Profile ◽  
Reducing Power ◽  
Total Content ◽  
Raw Material ◽  
Cocoa Beans ◽  
High Antioxidant Activity

Cocoa nibs ( cacao L.) are the cocoa beans after being fermented, dried, broken into small pieces, and then toasted. The cocoa nib composition is primordial for producers, industry, and final consumers because it affects the final quality of the raw material and any derivative product. The chemical composition was analyzed using , , , and Scharrer-Kurschner methods. The results showed that the cocoa nibs studied were rich in lipids (44.02 ± 5.22%), followed by carbohydrates (25.30%), raw fiber (12.85 ± 3.67%), protein (10.73 ± 1.80%), and ash content (2.58 ± 0.15%). The fatty acid profile was determined by gas chromatography and showed the presence of , , and acids. The low moisture content (4.52 ± 0.12%) and total acidity (2.25 ± 0.45 NaOH 100 g-1) contributed to the low count of microorganisms, which may give cocoa nibs a longer shelf life. Additionally, it presented high antioxidant activity, with a total content of 843.45 ± 11.85 mg acid / 100 g extract and 89.42 ± 0.02% iron reducing power.

scholarly journals COMPARATIVE IN VITRO ANTIOXIDANT ACTIVITY OF ETHYL ACETATE AND ETHANOL EXTRACTS OF CALLICARPA MACROPHYLLA

2021 ◽  
pp. 31-35
Author(s):  
GANESH N SHARMA ◽  
HARJINDER KAUR ◽  
BIRENDRA SHRIVASTAVA ◽  
SATISH CHANDER ARORA
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
Ethyl Acetate Extract ◽  
Reducing Power ◽  
Total Content ◽  
Ethanol Extract ◽  
World Health ◽  
Ethanol Extracts ◽  
In Vitro Antioxidant Activity

Objective: The objective of the study was to evaluate and compare the antioxidant activity of (EA and E) ethyl acetate and ethanol extracts of (CM) Callicarpa macrophylla. Methods: The physiochemical parameters were assessed according to guidelines given by the world health organization. The total content of phenols and flavonoids was assessed by Folin–Ciocalteu and aluminum chloride methods. In vitro, antioxidant activity was screened by (DPPH) 1, 1-diphenyl-2- picrylhydrazyl and(H2O2) hydrogen peroxide scavenging and reducing power assay. Results: The physicochemical parameters fulfilled the standards of WHO guidelines. Total phenol and flavonoid content were more in ethanol extract as compared to ethyl acetate extract of CM. The antioxidant activity of ethanol extract was further high as compared to ethyl acetate extract of Callicarpa macrophylla. The IC50 of Callicarpa macrophylla ethanol extract was less than the ethyl acetate extract. So, more antioxidant activity of ethanol extract compared to ethyl acetate extract of CM. Conclusion: Overall, both the extracts showed antioxidant activity and can be used further for diseases that can be managed using antioxidants. Ethanol extract possessed significant antioxidant effects than the ethyl acetate extract.

Evaluation of Antioxidant Activity of Rhizome Extract of Paris polyphylla Smith

2017 ◽  
Vol 24 (3) ◽  
pp. 147-151
Author(s):  
Jyoti Sati ◽  
Vijay Trivedi ◽  
Dharam Attri ◽  
Mohan Nautiyal
Keyword(s):  
Antioxidant Activity ◽  
Therapeutic Agent ◽  
Antioxidant Properties ◽  
Reducing Power ◽  
Biological Properties ◽  
Total Phenolic ◽  
Traditional Medicines ◽  
Enzymatic Antioxidant ◽  
Potential Source ◽  
Paris Polyphylla

The paper evaluates the antioxidant activity of extract of rhizomes of Paris polyphylla Smith. The present study was undertaken to appraise the non enzymatic antioxidant properties of Paris polyphylla. It is an important medicinal herb widely used in traditional medicines such as antihelmintic, antispasmodic, digestive, stomachic, expectorant, vermifuge and antidote against snake bite and also has the biological properties like anticancer, antimicrobial, anti-tumour, cytotoxicity and steroidal etc. The antioxidant activity of this plant was assessed by 2, 2-diphenyl picryl hydrazyl (DPPH), Total Phenolic content, SOD scavenging activity and total reducing power. The main objective of this study is, to evaluate the level of non enzymatic antioxidant properties of P. polyphylla that will be a potential source of natural antioxidant and a therapeutic agent in preventing the oxidative stress related diseases.

scholarly journals Parameters of antioxidant activity of Galega officinalis L. and Galega orientalis Lam. (Fabaceae Lindl.) plant raw material

10.5219/1271 ◽  
2020 ◽  
Vol 14 ◽  
pp. 125-134
Author(s):  
Olena Vergun ◽  
Oksana Shymanska ◽  
Dzhamal Rakhmetov ◽  
Olga Grygorieva ◽  
Eva Ivanišová ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Phenolic Acids ◽  
Pharmacological Study ◽  
Reducing Power ◽  
Total Content ◽  
Raw Material ◽  
Galega Officinalis ◽  
Dpph Method ◽  
Galega Orientalis ◽  
Plant Raw Material

The plant raw material of Galega officinalis L. (goat’s rue) and Galega orientalis Lam. (fodder galega) investigated in this study. These species are known as fodder crops with high productivity of green mass and as medicine plants. The current study was aimed to evaluate an accumulation in dry raw of selected plants the total content of phenolic acids (TPA) and flavonoids (TFC) as compounds with antioxidant activity (AA) by spectrophotometric method. AA by DPPH-method and phosphomolybdenum method (reducing power (RP)) was measured. Study of ethanolic extracts of G. officinalis showed accumulation of TPA in different organs in range from 3.65 to 15.17 mg.g-1 caffeic acid equivalent (CAE) and TFC from 10.08 to 65.75 mg.g-1 quercetin equivalent (QE), AA by DPPH-method from 6.02 to 8.45 mg.g-1 Trolox equivalent (TE) and RP of extracts by phosphomolybdenum method from 86.56 to 288.15 mg TE.g-1. In extracts of G. orientalis was identified TPA from 3.52 to 18.52 mg CAE.g-1 and TFC from 6.09 to 46.72 mg QE.g-1, antioxidant activity by DPPH-method from 6.80 to 8.48 mg TE.g-1 and antioxidant capacity by phosphomolybdenum method from 52.52 to 188.51 mg TE.g-1. It was established that less concentration of studied compounds found in the stems for both species. It should be noted that the content of phenolic acids in the leaves was decreased and flavonoids in stems increased during vegetation for both species. Content of phenolic acids in the generative organs and flavonoids in the leaves decreased in raw of G. orientalis during vegetation. Pearson’s correlation analysis demonstrated very strong relations between TFC and AA by DPPH, TPA and RP, TFC and RP for G. officinalis extracts. Very strong correlation in the extracts of G. orientalis found between TFC and RP, TPA and RP. Obtained results can be used in the further biochemical and pharmacological study.

Antioxidant Activity, Phenolic Content and Hematoprotective Effects of Cleome arabica Polyphenolic Leaf Extract

2019 ◽  
Author(s):  
C. Tigrine ◽  
A. Kameli
Keyword(s):  
Antioxidant Activity ◽  
Biological Effects ◽  
Reducing Power ◽  
Hematological Toxicity ◽  
Protective Effects ◽  
Ferrous Ions ◽  
Polyphenolic Extract ◽  
Cleome Arabica

In this study a polyphenolic extract from Cleome arabica leaves (CALE) was investigated for its antioxidant activity in vitro using DPPH•, metal chelating and reducing power methods and for its protective effects against AraC-induced hematological toxicity in vivo using Balb C mice. Results indicated that CALE exhibited a strong and dose-dependent scavenging activity against the DPPH• free radical (IC50 = 4.88 μg/ml) and a high reducing power activity (EC50 = 4.85 μg/ml). Furthermore, it showed a good chelating effects against ferrous ions (IC50 = 377.75 μg/ml). The analysis of blood showed that subcutaneous injection of AraC (50 mg/kg) to mice during three consecutive days caused a significant myelosupression (P < 0.05). The combination of CALE and AraC protected blood cells from a veritable toxicity. Where, the number of the red cells, the amount of hemoglobin and the percentage of the hematocrite were significantly high. On the other hand, AraC cause an elevation of body temperature (39 °C) in mice. However, the temperature of the group treated with CALE and AraC remained normal and did not exceed 37.5 °C. The observed biological effects of CALE, in vitro as well as in vivo, could be due to the high polyphenol and flavonoid contents. In addition, the antioxidant activity of CALE suggested to be responsible for its hematoprotective effect.

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