Podocarpo, a plant of ornamental interest: asexual propagation

Pistacia lentiscus L. is a dioecious shrub or small tree with high drought resistance, native in the entire Mediterranean basin. The variety ‘Chia’ (mastic tree) is cultivated for the production of mastic gum (a resin produced after wounding of the trunks and thick branches) in the Island of Chios (Greece), but it also has a significant ornamental value. In the present study, ten male and ten female genotypes of P. lentiscus var. Chia from the natural habitats of Chios were selected and examined with respect to the rooting efficiency of their shoot cuttings. The germination ability of the seeds was also tested. The 20 plants were morphologically described, mainly with respect to traits related to their ornamental value. Furthermore, leaves were collected from the 20 genotypes, from which genomic DNA (gDNA) was isolated, followed by amplification of gDNA fragments using the polymerase chain reaction (PCR) and inter simple sequence repeat (ISSR) primers. This was done aiming both at the determination of their genetic distance and the establishment of possible correlations between the amplified bands and certain morphological traits. The results of the study showed that there were differences among the genotypes for both methods of propagation. Regarding the rooting of the shoot cuttings, the best genotype was 8M (cv. ‘Psilophyllos’), achieving a rooting rate of 62.5% in winter with the application of indolebutyric acid (potassium salt of IBA), while regarding the seed germination capacity the genotype 2F exhibited the highest germination rate (57%). Genetic analysis using ISSRs separated the plants into four groups, one group consisting of male genotypes, one of the female genotypes, one consisting of members from both genders and a fourth containing a single male genotype. The genetic analysis of the male genotypes only produced a dendrogram showing the cultivars clustering in three different groups. Regardless of the genetic analysis, it seems that there were correlations between the ISSR markers and the leaf traits and also the gender and the asexual propagation. These correlations can assist future breeding programs of P. lentiscus var. Chia.

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Sequence data from isolated lichen-associated melanized fungi enhance delimitation of two new lineages within Chaetothyriomycetidae

Mycological Progress ◽

10.1007/s11557-021-01706-8 ◽

2021 ◽

Vol 20 (7) ◽

pp. 911-927

Author(s):

Lucia Muggia ◽

Yu Quan ◽

Cécile Gueidan ◽

Abdullah M. S. Al-Hatmi ◽

Martin Grube ◽

...

Keyword(s):

Sequence Data ◽

Single Species ◽

Sister Group ◽

Asexual Propagation ◽

Dna Sequence Data ◽

Wide Range ◽

The Family ◽

Rock Inhabiting Fungi ◽

Stable Habitat

AbstractLichen thalli provide a long-lived and stable habitat for colonization by a wide range of microorganisms. Increased interest in these lichen-associated microbial communities has revealed an impressive diversity of fungi, including several novel lineages which still await formal taxonomic recognition. Among these, members of the Eurotiomycetes and Dothideomycetes usually occur asymptomatically in the lichen thalli, even if they share ancestry with fungi that may be parasitic on their host. Mycelia of the isolates are characterized by melanized cell walls and the fungi display exclusively asexual propagation. Their taxonomic placement requires, therefore, the use of DNA sequence data. Here, we consider recently published sequence data from lichen-associated fungi and characterize and formally describe two new, individually monophyletic lineages at family, genus, and species levels. The Pleostigmataceae fam. nov. and Melanina gen. nov. both comprise rock-inhabiting fungi that associate with epilithic, crust-forming lichens in subalpine habitats. The phylogenetic placement and the monophyly of Pleostigmataceae lack statistical support, but the family was resolved as sister to the order Verrucariales. This family comprises the species Pleostigma alpinum sp. nov., P. frigidum sp. nov., P. jungermannicola, and P. lichenophilum sp. nov. The placement of the genus Melanina is supported as a lineage within the Chaetothyriales. To date, this genus comprises the single species M. gunde-cimermaniae sp. nov. and forms a sister group to a large lineage including Herpotrichiellaceae, Chaetothyriaceae, Cyphellophoraceae, and Trichomeriaceae. The new phylogenetic analysis of the subclass Chaetothyiomycetidae provides new insight into genus and family level delimitation and classification of this ecologically diverse group of fungi.

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INVESTIGATIONS ON FORAGE YIELD OF OAT GENOTYTPES UNDER IRRIGATED CONDITIONS OF KONYA

Journal of Global Innovations in Agriculture Sciences ◽

10.22194/jgias/9.9465 ◽

2021 ◽

Vol 9 (2) ◽

pp. 85-88

Author(s):

Muhammad Ahsan Qureshi ◽

Waqar Shafqat

Keyword(s):

Mangifera Indica ◽

Forage Yield ◽

Root Induction ◽

Shoot Induction ◽

Tropical Fruits ◽

Asexual Propagation ◽

Randomized Design ◽

Completely Randomized Design ◽

High Concentrations ◽

Direct Shooting

Shoot tips of newly emerging mango shoots were used as an explant and cultured on MS media for direct shooting. Different plant growth regulators i.e. BAP, NAA and IAA in varying concentrations were added to basal MS media. Experiment was laid out according to Completely Randomized Design (CRD) with four treatments and each treatment was replicated thrice. Maximum shoot induction (55.56%) was observed with 1 mg/L of BAP. Maximum mortality (83.30%) was observed when maximum concentration of BAP (3 mg/L) was used. Minimum days (12.633) to induce shoots were observed on MS media supplemented with 1 mg/L of BAP. Maximum number of shoots (2.50) were recorded in MS media supplemented with 1 mg/L of BAP. Low concentration of NAA initiated roots earlier in regenerated shoots as compare to high concentrations of NAA and IAA. MS media supplemented with NAA (1 mg/L) took less days (21 days) to induce roots. The combination of auxins (MS+ NAA 3 mg /L + IAA 1 mg /L) proved the best for root induction (38.87%). Keywords: Mangifera indica, tropical fruits, asexual propagation, plant tissue culture, micropropagation, phenolic exudation, acclimatization.

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Asexual Propagation of Eggplant (Solanum melongena L.) through Encapsulated Axillary Buds

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v19i2.5445 ◽

1970 ◽

Vol 19 (2) ◽

pp. 263-288 ◽

Cited By ~ 2

Author(s):

A.K.M. N. Huda ◽

, M.A. Bari ◽

M. Rahman

Keyword(s):

Key Words ◽

Plant Tissue ◽

Short Communication ◽

Solanum Melongena ◽

Axillary Buds ◽

Synthetic Seed ◽

Asexual Propagation

Key words: Auxillary buds, Eggplant, Encapsulation, Synthetic seed D.O.I. 10.3329/ptcb.v19i2.5445 Plant Tissue Cult. & Biotech. 19(2): 263-288, 2009 (December) - Short communication

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Key Regulatory Pathways, MicroRNAs, and Target Genes Participate in Adventitious Root Formation of Acer Rubrum L

10.21203/rs.3.rs-793059/v2 ◽

2021 ◽

Author(s):

Wenpeng Zhu ◽

Manyu Zhang ◽

Jianyi Li ◽

Hewen Zhao ◽

Kezhong Zhang ◽

...

Keyword(s):

Transcriptional Activation ◽

High Throughput Sequencing ◽

Target Genes ◽

Seedling Survival ◽

Expression Patterns ◽

Economic Value ◽

Acer Rubrum ◽

Pcr Analysis ◽

Asexual Propagation ◽

Regulatory Pathways

Abstract BackgroundAcer rubrum L. is a colorful ornamental tree with great economic value. Because this tree is difficult to root under natural conditions and the seedling survival rate is low, vegetative propagation methods are often used. Because the formation of adventitious roots (ARs) is essential for the survival of asexual propagation of A. rubrum, it is necessary to investigate the molecular regulatory mechanisms in the formation of ARs of A. ruburm. To address this knowledge gap, we sequenced the transcriptome and sRNA of the A. rubrum variety ‘Autumn Fantasy’ using high-throughput sequencing and explored changes in gene and microRNA (miRNA) expression in response to exogenous auxin treatment. ResultsWe identified 82,468 differentially expressed genes between the treated and untreated ARs, as well as 48 known and 95 novel miRNAs. We also identified 172 target genes of the known miRNAs using degradome sequencing. Two regulatory pathways (ubiquitin mediated proteolysis and plant hormone signal transduction), Ar-miR160a and the target gene ArARF10 were shown to be involved in the auxin response. We further investigated the expression patterns and regulatory roles of ArARF10 through subcellular localization, transcriptional activation, plant transformation, qRT-PCR analysis, and GUS staining. ConclusionsDifferential expression patterns indicated the Ar-miR160a-ArARF10 interaction might play a significant role in the regulation of AR formation in A. rubrum. Our study provided new insights into mechanisms underlying the regulation of AR formation in A. rubrum.

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Effective asexual propagation (fragmentation) method of the soft coral Xenia umbellata v1 (protocols.io.bsidnca6)

protocols.io ◽

10.17504/protocols.io.bsidnca6 ◽

2021 ◽

Author(s):

Sohyoung not provided ◽

Christian Wild ◽

Arjen Tilstra

Keyword(s):

Soft Coral ◽

Asexual Propagation

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Sexual and Vegetative Propagation of Hypericum empetrifolium Willd. subsp. empetrifolium

Notulae Botanicae Horti Agrobotanici Cluj-Napoca ◽

10.15835/nbha47111267 ◽

2018 ◽

Vol 47 (2) ◽

pp. 282-287

Author(s):

Anastasia AKOUMIANAKI-IOANNIDOU ◽

Ekaterini GERASIMIDOU ◽

Alexandra SALTA ◽

Ioannis ROUSSIS ◽

Dimitrios BILALIS

Keyword(s):

Significant Interaction ◽

Incubation Temperature ◽

Tap Water ◽

Hormone Treatment ◽

Germination Percentage ◽

Stem Cuttings ◽

Treatment Control ◽

Asexual Propagation ◽

Different Types ◽

Pharmaceutical Properties

Hypericum empetrifolium Willd. subsp. empetrifolium is an evergreen small shrub with small elongated decorative leaves and small yellow flowers in inflorescences, characterized for several pharmaceutical properties. In the present study, a first approach on the sexual and asexual propagation of this species was performed. Seeds, subjected to different types of pre-treatments [soaked in tap water; 50, 100 or 150 mg L-1 GA3 for 30 min and no treatment (control)], cultured for germination in petri dishes at 5, 10, 15, 20, 25, and 30 °C. Seed germination was only affected by temperature and the best result was obtained at 15 °C (71.2%). A significant interaction was found between pre-treatments and incubation temperature with the highest germination percentage (82%) occurred when the seeds soaked in 100 mg L-1 GA3 solution for 30 min and incubated at 20 °C constant temperature. Moreover, the germination speed was fastest from 20 to 25 °C (T50= 9.84 and 9.56 days for 20 and 25 °C, respectively). For asexual propagation, apical stem cuttings were taken at four different periods (4 seasons) and treated with IBA at concentrations of 0, 1000, 2000 and 3000 mg L-1. The cuttings were planted in a peat/perlite mixture 1:1 v/v in plastic square plug trays in order to study the rooting percentage. Winter was the most appropriate season for cuttings collection (100% rooting percentage) and dipping in 1000 or 2000 mg L-1 IBA (72% and 73%, respectively) was the best rooting hormone treatment.

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Propagation of Annonaceous plants

Revista Brasileira de Fruticultura ◽

10.1590/0100-29452019500 ◽

2019 ◽

Vol 41 (1) ◽

Cited By ~ 1

Author(s):

Gisela Ferreira ◽

Iván De-La-Cruz-Chacón ◽

Carmen Silvia Fernandes Boaro ◽

Daniel Baron ◽

Eurico Eduardo Pinto de Lemos

Keyword(s):

Management Strategies ◽

Economic Importance ◽

Tissue Formation ◽

Compatibility Studies ◽

Asexual Propagation ◽

Expression Of Genes ◽

Custard Apple ◽

Sexual Propagation ◽

Exogenous Factors ◽

The Subject

Abstract This review aims to present advances in studies on the propagation of the Annonaceae species, which includes species of economic importance such as: soursop, custard apple, atemoya and cherimoya. In sexual propagation, advances are mainly related to a better understanding of the stages of seed development, dormancy mechanisms, and germination. In asexual propagation, compatibility studies between grafts and rootstocks are presented, focusing on the expression of genes involved in tissue formation. The cutting method is also discussed, which is another option for the propagation for this group of plants considered difficult to root, approaching endogenous and exogenous factors related to the subject, as well as management strategies that affect the success of this technique.

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