scholarly journals The influence of nicotine in healing of small bowel anastomoses in rats: angiogenesis and miofibroblasts

2016 ◽  
Vol 43 (2) ◽  
pp. 87-92 ◽  
Author(s):  
James Skinovsky ◽  
Osvaldo Malafaia ◽  
Mauricio Chibata ◽  
Fernanda Tsumanuma ◽  
Flávio Panegalli Filho ◽  
...  
Keyword(s):  
Small Bowel ◽  
Blood Vessels ◽  
Immunohistochemical Staining ◽  
Wistar Rats ◽  
Time Interval ◽  
Time Intervals ◽  
Duodenojejunal Flexure ◽  
Smooth Muscle Alpha Actin ◽  
Alpha Actin ◽  
And Control

ABSTRACT Objective: to know the effect of nicotine on angiogenesis and myofibroblast formation in anastomoses of the small bowel of rats. Methods: we randomly divided 60 Wistar rats into the groups Nicotine (N) and control (C), according to the proposed treatment. Each group was subdivided into three subgroups according to the time interval used for the evaluation (7, 14 or 28 days). The N group with 30 animals received nicotine subcutaneously at a dose of 2mg/kg body weight, diluted in 0.3ml of 0.9% saline, twice daily for 28 days prior to the operation, and for more 7, 14 or 28 days, depending on the subgroup. The C group (also 30 animals) received only saline on the same conditions and time intervals. After 28 days we carried out an end-to-end anastomosis 10cm distal to the duodenojejunal flexure in each rat. After 7, 14 or 28 days after surgery, we euthanized ten animals of each group, sent specimens of the anastomosis areas, 1cm proximal to 1cm distal, to counting of blood vessels and myofibroblasts through immunohistochemical staining by the application of monoclonal anti-factor VIII antibodies and anti-smooth muscle alpha-actin. Results: the administration of nicotine led to the decrease in the number of blood vessels measured on the 28th postoperative day and the number of myofibroblasts measured on the seventh day following completion of the anastomoses. Conclusion: administration of nicotine was deleterious on angiogenesis and myofibroblast formation in rats' small intestine anastomoses.

scholarly journals The effect of diminazene aceturate on cholinesterase activity in dogs with canine babesiosis

1997 ◽  
Vol 68 (4) ◽  
Author(s):  
R.J. Milner ◽  
F. Reyers ◽  
J.H. Taylor ◽  
J.S. Van den Berg
Keyword(s):  
Treatment Group ◽  
Underlying Mechanism ◽  
Time Interval ◽  
Control Groups ◽  
Canine Babesiosis ◽  
Diminazene Aceturate ◽  
Time Intervals ◽  
Significant Depression ◽  
The Difference ◽  
And Control

A clinical trial was designed to evaluate the effects of diminazene aceturate and its stabiliser antipyrine on serum pseudocholinesterase (PChE) and red blood cell acetylcholinesterase (RBC AChE) in dogs with babesiosis. The trial was conducted on naturally occurring, uncomplicated cases of babesiosis (n = 20) that were randomly allocated to groups receiving a standard therapeutic dose of diminazene aceturate with antipyrine stabiliser (n = 10) or antipyrine alone (n = 10). Blood was drawn immediately before and every 15 minutes for 1 hour after treatment. Plasma PChE showed a 4 % decrease between 0 and 60 min within the treatment group (p < 0.05). No statistically significant differences were found between the treatment and control groups at any of the time intervals for PChE. There was an increase in RBC AChE activity at 15 min in the treatment group (p < 0.05). No significant differences were found between the treatment and control groups at any time interval for RBC AChE. In view of the difference in PChE, samples from additional, new cases (n = 10) of canine babesiosis were collected to identify the affect of the drug over 12 hours. No significant depression was identified over this time interval. The results suggests that the underlying mechanism in producing side-effects, when they do occur, is unlikely to be through cholinesterase depression.

scholarly journals Light-emitting diode exposure enhances sperm motility in men with and without asthenospermia: preliminary results

2015 ◽  
Vol 87 (1) ◽  
pp. 14 ◽  
Author(s):  
Nader Salama ◽  
Mohamed El-Sawy
Keyword(s):  
Sperm Motility ◽  
Intrauterine Insemination ◽  
Light Emitting Diode ◽  
Blue Staining ◽  
Time Interval ◽  
Time Intervals ◽  
Light Emitting ◽  
Red Led ◽  
And Control

Objective: To evaluate the effect of lightemitting diode (LED) on sperm motility in men with and without asthenospermia. Material and Methods: Semen samples from 27 men were assessed and washed. An aliquot was taken from each sample as a control. The remaining amount was exposed to red LED for 2, 5 and 10 minutes. Sperm motility from the test and control tubes were re-checked at the end of each time interval. In 11 of these 27 samples, the same protocol was repeated without sperm washing. Evaluation of sperm creatine kinase (CK) activity, hypoosmotic swelling (HOS) test and aniline blue staining (ANBS) were undertaken after phototherapy in additional 15 samples. Results: Progressive sperm motility increased significantly after LED treatment at the different time intervals whether in washed (p = 0.000) or non-washed (p = 0.003) samples. The amount of the increase in motility in washed aliquots was significantly more (p = 0.000) than in naive semen. Sperm CK activity increased, but was not significant whilst there were no changes regarding HOS and ANBS. Conclusion: Red LED is a promising safe tool to boost sperm motility in vitro. This may have a great implication on maximizing the possibilities and outcomes of intrauterine insemination trials.

scholarly journals THE PHARMACOLOGICAL ACTION OF CHOLINERGIC SUBSTANCES ON NEW CORNEAL BLOOD VESSELS IN RATS

2021 ◽  
Vol 21 (1) ◽  
pp. 235-246
Author(s):  
DANIELA BIANCA DAMIAN DAMIAN ◽  
SANZIANA ISTRATE ◽  
MIHAIL ZEMBA ◽  
IOANA CRISTINA COMAN ◽  
AURELIAN MIHAI GHITA ◽  
...  
Keyword(s):  
Body Weight ◽  
Blood Vessels ◽  
Muscarinic Receptors ◽  
Wistar Rats ◽  
Pharmacological Action ◽  
Video Camera ◽  
Time Intervals ◽  
Rat Pups ◽  
Total Magnification ◽  
Microsoft Office

This study aimed to evaluate the existence of muscarinic receptors in the new corneal blood vessels, experimentally induced with ketamine in rat pups. The experimental model of neovascularization was performed on 15-day-old Wistar rats, in which 5 intraperitoneal injections of ketamine were administered at a dose of 150 mg/kg body weight at 5-day intervals. Examination of the reactivity of new corneal blood vessels was performed on day 45 of life. Each eye that developed neovascularization was evaluated with a Nikon stereomicroscope, coupled to a Mshot video camera, and the total magnification of the system was 400X. The vascular diameter was measured at a chosen point, the same point /points for each recording made to that eye, and the acquisition of images was performed at set time intervals, every 60 seconds for each eye to be examined, throughout the recording period. The parameters under investigation were the variations of the vascular diameter, and the processing of the obtained data was performed with the help of Microsoft Office Excel. Our results suggest that muscarinic receptors are present in the new corneal blood vessels because the administration of conjunctival instillations of acetylcholine caused statistically significant vasodilation, while atropine, a blocker of muscarinic receptors, antagonized this effect.

scholarly journals Quantitative assessment of the effectiveness of joint measures led by Fangcang shelter hospitals in response to COVID-19 epidemic in Wuhan, China

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hui Jiang ◽  
Pengfei Song ◽  
Siyi Wang ◽  
Shuangshuang Yin ◽  
Jinfeng Yin ◽  
...  
Keyword(s):  
Prevention And Control ◽  
Quantitative Assessment ◽  
Time Dependent ◽  
Time Interval ◽  
Cumulative Number ◽  
Hospital Visit ◽  
Time Intervals ◽  
Illness Onset ◽  
Reproduction Numbers ◽  
And Control

Abstract Objective To quantitatively evaluate the effectiveness of Fangcang shelter hospitals, designated hospitals, and the time interval from illness onset to diagnosis toward the prevention and control of the COVID-19 epidemic. Methods We used SEIAR and SEIA-CQFH warehouse models to simulate the two-period epidemic in Wuhan and calculate the time dependent basic reproduction numbers (BRNs) of symptomatic infected individuals, asymptomatic infected individuals, exposed individuals, and community-isolated infected individuals. Scenarios that varied in terms of the maximum numbers of open beds in Fangcang shelter hospitals and designated hospitals, and the time intervals from illness onset to hospitals visit and diagnosis were considered to quantitatively assess the optimal measures. Results The BRN decreased from 4.50 on Jan 22, 2020 to 0.18 on March 18, 2020. Without Fangcang shelter hospitals, the cumulative numbers of cases and deaths would increase by 18.58 and 51.73%, respectively. If the number of beds in the designated hospitals decreased by 1/2 and 1/4, the number of cumulative cases would increase by 178.04 and 92.1%, respectively. If the time interval from illness onset to hospital visit was 4 days, the number of cumulative cases and deaths would increase by 2.79 and 6.19%, respectively. If Fangcang shelter hospitals were not established, the number of beds in designated hospitals reduced 1/4, and the time interval from visiting hospitals to diagnosis became 4 days, the cumulative number of cases would increase by 268.97%. Conclusion The declining BRNs indicate the high effectiveness of the joint measures. The joint measures led by Fangcang shelter hospitals are crucial and need to be rolled out globally, especially when medical resources are limited.

scholarly journals On the turnpike property with interior decay for optimal control problems

2021 ◽  
Author(s):  
Martin Gugat
Keyword(s):  
Optimal Control ◽  
Differential Equations ◽  
Objective Function ◽  
Optimal Control Problems ◽  
Terminal State ◽  
Control Problems ◽  
Time Interval ◽  
Time Intervals ◽  
And Control ◽  
State And Control Constraints

AbstractIn this paper the turnpike phenomenon is studied for problems of optimal control where both pointwise-in-time state and control constraints can appear. We assume that in the objective function, a tracking term appears that is given as an integral over the time-interval $$[0,\, T]$$ [ 0 , T ] and measures the distance to a desired stationary state. In the optimal control problem, both the initial and the desired terminal state are prescribed. We assume that the system is exactly controllable in an abstract sense if the time horizon is long enough. We show that that the corresponding optimal control problems on the time intervals $$[0, \, T]$$ [ 0 , T ] give rise to a turnpike structure in the sense that for natural numbers n if T is sufficiently large, the contribution of the objective function from subintervals of [0, T] of the form $$\begin{aligned} {[}t - t/2^n,\; t + (T-t)/2^n] \end{aligned}$$ [ t - t / 2 n , t + ( T - t ) / 2 n ] is of the order $$1/\min \{t^n, (T-t)^n\}$$ 1 / min { t n , ( T - t ) n } . We also show that a similar result holds for $$\epsilon $$ ϵ -optimal solutions of the optimal control problems if $$\epsilon >0$$ ϵ > 0 is chosen sufficiently small. At the end of the paper we present both systems that are governed by ordinary differential equations and systems governed by partial differential equations where the results can be applied.

Evidence for a Blood Ganglion Barrier in the Superior Cervical Ganglion of the Rat

1982 ◽  
Vol 40 ◽  
pp. 204-204
Author(s):  
D. M. DePace
Keyword(s):  
Blood Vessels ◽  
Superior Cervical Ganglion ◽  
Peripheral Nerves ◽  
Time Schedule ◽  
Transmission Electron ◽  
Cervical Ganglion ◽  
The Central Nervous System ◽  
Cervical Ganglia ◽  
Capillary Circulation ◽  
And Control

The majority of blood vessels in the superior cervical ganglion possess a continuous endothelium with tight junctions. These same features have been associated with the blood brain barrier of the central nervous system and peripheral nerves. These vessels may perform a barrier function between the capillary circulation and the superior cervical ganglion. The permeability of the blood vessels in the superior cervical ganglion of the rat was tested by intravenous injection of horseradish peroxidase (HRP). Three experimental groups of four animals each were given intravenous HRP (Sigma Type II) in a dosage of.08 to.15 mg/gm body weight in.5 ml of.85% saline. The animals were sacrificed at five, ten or 15 minutes following administration of the tracer. Superior cervical ganglia were quickly removed and fixed by immersion in 2.5% glutaraldehyde in Sorenson's.1M phosphate buffer, pH 7.4. Three control animals received,5ml of saline without HRP. These were sacrificed on the same time schedule. Tissues from experimental and control animals were reacted for peroxidase activity and then processed for routine transmission electron microscopy.

Trans-resveratrol supplement lowers lipid peroxidation responses of exercise in male Wistar rats

2020 ◽  
pp. 1-6 ◽  
Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Wistar Rats ◽  
Enzymatic Antioxidants ◽  
Acute Response ◽  
Total Antioxidant ◽  
Male Wistar Rats ◽  
Exercise Induced ◽  
And Control

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.

METABOLIC STUDIES WITH 131I-LABELED THYROXINE. II.

1963 ◽  
Vol 44 (3) ◽  
pp. 475-480 ◽  
Author(s):  
R. Grinberg
Keyword(s):  
Spinal Cord ◽  
Central Nervous System ◽  
Nervous System ◽  
Optic Nerve ◽  
Pituitary Gland ◽  
Time Interval ◽  
Time Intervals ◽  
Pituitary Glands ◽  
The Central Nervous System ◽  
Tentative Explanation

ABSTRACT Radiologically thyroidectomized female Swiss mice were injected intraperitoneally with 131I-labeled thyroxine (T4*), and were studied at time intervals of 30 minutes and 4, 28, 48 and 72 hours after injection, 10 mice for each time interval. The organs of the central nervous system and the pituitary glands were chromatographed, and likewise serum from the same animal. The chromatographic studies revealed a compound with the same mobility as 131I-labeled triiodothyronine in the organs of the CNS and in the pituitary gland, but this compound was not present in the serum. In most of the chromatographic studies, the peaks for I, T4 and T3 coincided with those for the standards. In several instances, however, such an exact coincidence was lacking. A tentative explanation for the presence of T3* in the pituitary gland following the injection of T4* is a deiodinating system in the pituitary gland or else the capacity of the pituitary gland to concentrate T3* formed in other organs. The presence of T3* is apparently a characteristic of most of the CNS (brain, midbrain, medulla and spinal cord); but in the case of the optic nerve, the compound is not present under the conditions of this study.

scholarly journals Neural Network Approach for Global Solar Irradiance Prediction at Extremely Short-Time-Intervals Using Particle Swarm Optimization Algorithm

Energies ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 1213
Author(s):  
Ahmed Aljanad ◽  
Nadia M. L. Tan ◽  
Vassilios G. Agelidis ◽  
Hussain Shareef
Keyword(s):  
Neural Networks ◽  
Particle Swarm Optimization ◽  
Solar Irradiance ◽  
Particle Swarm ◽  
Time Interval ◽  
Time Intervals ◽  
Swarm Optimization ◽  
Backpropagation Neural Networks ◽  
Proposed Model ◽  
Short Time

Hourly global solar irradiance (GSR) data are required for sizing, planning, and modeling of solar photovoltaic farms. However, operating and controlling such farms exposed to varying environmental conditions, such as fast passing clouds, necessitates GSR data to be available for very short time intervals. Classical backpropagation neural networks do not perform satisfactorily when predicting parameters within short intervals. This paper proposes a hybrid backpropagation neural networks based on particle swarm optimization. The particle swarm algorithm is used as an optimization algorithm within the backpropagation neural networks to optimize the number of hidden layers and neurons used and its learning rate. The proposed model can be used as a reliable model in predicting changes in the solar irradiance during short time interval in tropical regions such as Malaysia and other regions. Actual global solar irradiance data of 5-s and 1-min intervals, recorded by weather stations, are applied to train and test the proposed algorithm. Moreover, to ensure the adaptability and robustness of the proposed technique, two different cases are evaluated using 1-day and 3-days profiles, for two different time intervals of 1-min and 5-s each. A set of statistical error indices have been introduced to evaluate the performance of the proposed algorithm. From the results obtained, the 3-days profile’s performance evaluation of the BPNN-PSO are 1.7078 of RMSE, 0.7537 of MAE, 0.0292 of MSE, and 31.4348 of MAPE (%), at 5-s time interval, where the obtained results of 1-min interval are 0.6566 of RMSE, 0.2754 of MAE, 0.0043 of MSE, and 1.4732 of MAPE (%). The results revealed that proposed model outperformed the standalone backpropagation neural networks method in predicting global solar irradiance values for extremely short-time intervals. In addition to that, the proposed model exhibited high level of predictability compared to other existing models.

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