scholarly journals Effect of Super-Oxidized Water, Sodium Hypochlorite and EDTA on Dentin Microhardness

2014 ◽  
Vol 25 (5) ◽  
pp. 420-424 ◽  
Author(s):  
Alexandre Corrêa Ghisi ◽  
Patrícia Maria Poli Kopper ◽  
Flávia E. R. Baldasso ◽  
Caroline P. Stürmer ◽  
Giampiero Rossi-Fedele ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Root Surface ◽  
Negative Control ◽  
Significance Level ◽  
Significant Difference ◽  
Control Root ◽  
Root Canal Dentin ◽  
The One ◽  
Cervical Root

The present study aimed to evaluate the influence of the following irrigating solutions on the microhardness of root canal dentin: 2% sodium hypochlorite (2NaOCl), 5% sodium hypochlorite (5NaOCl), super-oxidized water (400 ppm Sterilox - Sx) and 17% EDTA (E). Eighty roots from bovine incisors were randomly divided into 8 groups (n=10): 2NaOCl, 5NaOCl, Sx, and 2NaOCl + E, 5NaOCl + E, Sx + E (associated with E as final irrigant for 5 min), E solely and distilled water (dH2O) as the negative control. Root canal preparation was performed by hand instruments, using one of the irrigation protocols for 30 min. Then, 5 mm of the cervical root third were cut out from each sample and subjected to the Vickers microhardness test, at two points, one at approximately 500-1000 µm from the root canal lumen (distance 1), and the other at approximately 500-1000 µm from the external root surface (distance 2). Data were analyzed by Wilcoxon and Kruskal-Wallis tests at 5% significance level. Microhardness values at distance 1 were significantly lower than those at distance 2 for all groups, except 5NaOCl and 5NaOCl + E groups (p>0.05). EDTA showed the lowest microhardness values. However, no statistically significant difference was detected among groups at distance 1 and EDTA was significantly different only from Sx at distance 2. In conclusion, all tested solutions showed lower microhardness at the most superficial root canal dentin layer compared to the one found near the external root surface, except 5NaOCl and 5NaOCl + E; EDTA promoted lower microhardness values in comparison to Sterilox at this site.

scholarly journals Comparison of Medicaments Used in Regenerative Endodontics in Terms of Dentin Microhardness and Adhesion of Mineral in Terms of Dentin Microhardness and Adhesion of Mineral Trioxide Aggregate Trioxide Aggregat

2020 ◽  
Vol 27 (3) ◽  
Author(s):  
Esma Saricam ◽  
◽  
Neslihan Bulak ◽  
Esra Özyurt ◽  
Suat Özcan ◽  
...  
Keyword(s):  
Bond Strength ◽  
Clavulanic Acid ◽  
Root Canal ◽  
Mineral Trioxide Aggregate ◽  
Significance Level ◽  
Regenerative Endodontics ◽  
Significant Difference ◽  
Amoxicillin Clavulanic Acid ◽  
Root Canal Dentin ◽  
Push Out

Eliminating microorganisms in the root canal system is important for the success of regenerative endodontics. Objective: This study evaluated the effects of different antibiotic pastes used for regenerative endodontic procedures on dentin microhardness and the push-out bond strength of mineral trioxide aggregate (MTA) to root canal dentin. Methods: Sixty-four maxillary central incisors were instrumented and randomly divided into the following four groups (n = 16) for medicament treatment: triple antibiotic paste, amoxicillin+clavulanic acid, cefaclor, and control (no dressing). After 21 days, two root segments were obtained by sectioning the roots horizontally for push-out and microhardness evaluations. MTA was placed into the root canal of the sectioned segment for the push-out test. In the microhardness evaluation, three indentations were made at 500 and 1,000 μm from the canal lumen. The arithmetic mean was then calculated for each distance. ANOVA with post hoc Scheffe test and t test were used for the statistical analyses. The significance level was set at p < 0.05. Results: No significant difference was found between the groups in terms of push-out bond strength (p > 0.05). Cefaclor and amoxicillin+clavulanic acid reduced the microhardness values of the dentin at 500 μm (p < 0.05) while cefaclor had the lowest value at 1,000 μm (p < 0.05). Conclusion: Cefaclor reduced the microhardness value more than the other medicaments did at a depth of 1,000 μm. The pastes provided similar adhesion of MTA.

scholarly journals Is pomegranate peels infusion effective for disinfection of toothbrushes?

2016 ◽  
Vol 45 (5) ◽  
pp. 253-257
Author(s):  
Priscila Lima de Luna FREIRE ◽  
Priscila Hernández CAMPOS ◽  
Fabíola Galbiatti CARVALHO ◽  
Jonas Almeida RODRIGUES ◽  
Michele Baffi DINIZ
Keyword(s):  
Sodium Hypochlorite ◽  
Culture Medium ◽  
Punica Granatum ◽  
Negative Control ◽  
Chlorhexidine Digluconate ◽  
Agar Culture ◽  
Significance Level ◽  
Colony Forming Unit ◽  
Significant Difference ◽  
Disinfectant Solution

Abstract Introduction Methods of decontamination or sanitization of toothbrushes have been questioned. Objective This study assessed the effectiveness of pomegranate peels infusion as a disinfectant of toothbrushes against Streptococcus mutans. Material and method A sample of 16 schoolchildren aged between 7 and 9 years performed brushing 5 days/week, with a careful brushing once a day. After each day of brushing, the toothbrushes were washed and sprayed with one disinfectant solution. This procedure was repeated for 4 weeks using one of the different solutions per week: distilled water (G1; negative control), pomegranate (Punica granatum Linn) peels infusion (G2), 1% sodium hypochlorite (G3) and 0.12% chlorhexidine digluconate (G4). After the fifth day, toothbrushes were collected for laboratory analysis. Toothbrushes heads were subjected to agitation in saline dilution of 10–1, 10–2,10–3, and 25 μL of each dilution were seeded in mitis salivarius agar culture medium for S. mutans colony-forming unit (CFU) counting. One calibrated examiner (Kappa = 0.91) performed the CFU (mL–1 × 104) counts. Kruskal-Wallis and Dunn Multiple Comparison tests were used at a significance level of 5%. Result G1 presented the highest number of CFU (3.9 ± 8.4), followed by G2 (3.2 ± 4.0). No S. mutans growth was observed in G3 and G4. There was no statistically significant difference between G1 and G2 and between G3 and G4 (p>0.05). Conclusion Pomegranate infusion was completely ineffective for the disinfection of toothbrushes against S. mutans when compared with 1% sodium hypochlorite and 0.12% chlorhexidine digluconate solutions.

scholarly journals INCIDENCE OF APICAL ROOT CRACK FORMATION AFTER ROTARY ROOT CANAL INSTRUMENTATION AT DIFFERENT INSTRUMENTATION LENGTHS USING PROTAPER UNIVERSAL, PROTAPER NEXT AND PROTAPER GOLD FILES - AN IN-VITRO STUDY

2021 ◽  
Vol 9 (11) ◽  
pp. 675-682
Author(s):  
Anvesha Parate ◽  
◽  
Siddheswaran V ◽  
Keyword(s):  
Root Canal ◽  
Crack Formation ◽  
Negative Control ◽  
Control Group ◽  
Blue Dye ◽  
Significance Level ◽  
Group I ◽  
Significant Difference ◽  
Root Crack

Aim: To evaluate the incidence of apical root crack formation after root canal preparation using ProTaper Universal (PTU), ProTaper Next (PTN) and ProTaper Gold (PTG) rotary file systems and compare the crack formation at various instrumentation lengths. Subjects and Methods: One hundred single rooted extracted mandibular premolar teeth were mounted in acrylic resin blocks after simulating periodontal ligament. The teeth were divided into four groups. Group I was instrumented using PTU, group II using PTN and group III using PTG rotary files, while group IV was left untreated, serving as a negative control group. Each group was subdivided into three subgroups: A, B and C instrumented till root canal length (RCL), RCL-1 and RCL+1. Root apex was sectioned horizontally 1-2mm from apical foramen and was stained with 1% methylene blue dye followed by stereomicroscopic evaluation to determine apical root cracks. The data was analyzed using chi-square, Shapiro Wilkinson and Cramers phi test. The significance level was set at P<0.05. Result: Significant difference was seen in percentage of cracks after instrumentation with PTU and PTG, while no significant difference with PTU and PTN. Specimen instrumented upto RCL-1 showed less cracks as compared to those instrumented upto RCL and RCL+1. Conclusion: PTG produced least number of cracks followed by PTN and PTU. Moreover, instrumentation at RCL-1 reduced the crack formation.

scholarly journals Quantitative evaluation of apically extruded debris during root canal instrumentation with ProTaper Universal, ProTaper Next, WaveOne, and self-adjusting file systems

2014 ◽  
Vol 08 (04) ◽  
pp. 504-508 ◽  
Author(s):  
Damla Ozsu ◽  
Ertugrul Karatas ◽  
Hakan Arslan ◽  
Meltem C. Topcu
Keyword(s):  
Root Canal ◽  
File Systems ◽  
Universal Group ◽  
Significance Level ◽  
Root Canals ◽  
Single File ◽  
Measurable Amount ◽  
Significant Difference ◽  
The One ◽  
Root Canal Instrumentation

ABSTRACT Objectives: The aim of this study was to compare the amount of apically extruded debris during preparation with ProTaper Universal (Dentsply Maillefer, Ballaigues, Switzerland), ProTaper Next (Dentsply Maillefer), a reciprocating single-file (WaveOne; VDW GmbH, Munich, Germany), and a self-adjusting file (SAF; ReDent Nova, Ra'anna, Israel). Materials and Methods: Fifty-six intact mandibular premolar teeth were randomly assigned to four groups. The root canals were prepared according to the manufacturers’ instructions using the ProTaper Universal, ProTaper Next, WaveOne, and SAF. Apically extruded debris was collected in preweighted Eppendorf tubes during instrumentation. The net weight of the apically extruded debris was determined by subtracting the preweights and postweights of the tubes. The data were statistically analyzed using the one-way analysis of variance and the least significant difference tests at a significance level of P < 0.05. Results: A measurable amount of debris was apically extruded in all groups, and the amounts of debris extrusion in the groups were statistically significant (P < 0.001). The ProTaper Next and WaveOne groups resulted in less debris extrusion than the ProTaper Universal group (P < 0.05), and the SAF group resulted in the least debris extrusion. Conclusions: Within the limitations of the present study, it can be concluded that all systems extruded debris beyond the apical foramen.

scholarly journals The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae

2010 ◽  
Vol 8 (1) ◽  
pp. 27-30
Author(s):  
TRI NUGROHO WIBOWO ◽  
DARUKUTNI DARUKUTNI ◽  
SUTARTINAH SRI HANDAYANI
Keyword(s):  
Aedes Aegypti ◽  
Castor Bean ◽  
Ricinus Communis ◽  
Negative Control ◽  
Probit Analysis ◽  
Control Group ◽  
Significance Level ◽  
Extract Concentration ◽  
Mortality Effect ◽  
Significant Difference

Wibowo TN, Darukutni, Handayani SS. 2010. The mortality effect of castor bean (Ricinus communis) extract on Aedes aegypti larvae. Biofarmasi 8: 77-81. The aim of this research was to determine the mortality effect of Ricinus communis L. extract on Aedes aegypti L. larvae. This research was an laboratory experimental, with a post-test only controlled group design, and used 750 larvae Instar III of A. aegypti L. that divided into 6 groups (control group, and five treatment groups consisted of 0.10% extract, 0.25% extract, 0.50% extract, 0.75% extract and 1% extract). The sampling technical was a purposive sampling method. The larvae were put into 25 ml experimental liquid for 24 hours. The observation was counting a number of dead larvae in 24 hours. Data were analyzed with one-way ANOVA test continued with Least Significant Difference (LSD) using SPSS for Windows Release statistically with a significance level p<0.05 then continued with a probit analysis. There were 0 larva death at negative control, 23.8 (95%) larvae death at 0.10% extract concentration, 24.6 (98%) larvae death at 0.25% extract concentration, 25.0 (100%) larvae death at 0.50%, 0.75% and 1.00% extract concentration. There was a significant difference in larvae death of A. aegypti in all groups. The LC50 of R. communis extract was 0.01036% (103.6 ppm), therefore it could be concluded that R. communis extract had a mortality effect to A. aegypti larvae.

scholarly journals Apical Debris Extrusion by Adaptive Root Canal Instrumentation in Oval Canals: Full-Sequence SAF System vs. the XP-Endo Shaper Plus Sequence

2020 ◽  
Vol 10 (16) ◽  
pp. 5684 ◽  
Author(s):  
Ajinkya M. Pawar ◽  
Bhaggyashri A. Pawar ◽  
Anuj Bhardwaj ◽  
Alexander Maniangat Luke ◽  
Zvi Metzger ◽  
...  
Keyword(s):  
Root Canal ◽  
Significance Level ◽  
Full Sequence ◽  
Apical Extrusion ◽  
Significant Difference ◽  
Group A ◽  
Group B ◽  
Stage 1 ◽  
Two Stages ◽  
Root Canal Instrumentation

Apical extrusion of debris (AED) by the full sequence of the self-adjusting file (SAF) system was compared with that of the XP-endo shaper plus sequence. Sixty permanent mandibular incisors were randomly assigned to two groups (n = 30) for root canal instrumentation: Group A: Stage 1—pre-SAF OS, pre-SAF 1 and pre-SAF 2 files, followed by Stage 2—1.5 mm SAF; and Group B: Stage 1— hand K-file 15/0.02, followed by Stage 2—XP-endo shaper and Stage 3—XP-endo finisher. The AED produced during instrumentation at each stage was collected in pre-weighed Eppendorf tubes. The weights of AED by the two methods were compared using t tests with significance level set at 5%. Group A produced significantly less total AED than Group B (p < 0.001), with no significant difference in debris extrusion between the two stages (p = 0.3014). Conversely, in Group B, a significant difference was noted between Stage 1 and Stages 2 and 3 (p < 0.01), with no significant difference between Stages 2 and 3 (p = 0.488). Both sequences resulted in measurable amounts of AED. Each phase, in either procedure, made its own contribution to the extrusion of debris.

scholarly journals Effect of a Surfactant on the Antimicrobial Activity of Sodium Hypochlorite Solutions

2014 ◽  
Vol 25 (5) ◽  
pp. 416-419 ◽  
Author(s):  
Marcos Rodolfo Bolfoni ◽  
Marcelo dos Santos Ferla ◽  
Otávio da Silva Sposito ◽  
Luciano Giardino ◽  
Rogério de Castilho Jacinto ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Sodium Hypochlorite ◽  
Root Canal ◽  
Root Canal Preparation ◽  
Human Teeth ◽  
Significance Level ◽  
Root Canals ◽  
Tukey Test ◽  
Irrigation Solution ◽  
Period Data

The objective of the present study was to evaluate the antimicrobial activity of sodium hypochlorite (NaOCl) associated with a surfactant. Seventy single-rooted extracted human teeth were inoculated with Enterococcus faecalis, and incubated for 21 days (37 °C). The groups were distributed according to the irrigation solution used during root canal preparation: 5%, 2.5% and 1% NaOCl; 5%, 2.5% and 1% Hypoclean(r), a solution containing a surfactant (cetrimide) associated with NaOCl. Three microbiological samples were collected from each tooth: S1 - before instrumentation; S2 - immediately after instrumentation; and S3 - after a seven-day period. Data were submitted to ANOVA and Tukey test with 5% significance level. The results showed that immediately after root canal preparation (S2), E. faecalis was eliminated in all the experimental groups. However, after 7 days (S3), only the groups in which Hypoclean was used, remained contamination-free, including Hypoclean associated with 1% NaOCl, while the root canals irrigated with 1% NaOCl only, presented the highest percentage of bacterial growth. In conclusion, the addition of surfactant increased the antimicrobial activity of 1% NaOCl to levels similar to 5% NaOCl.

Effect of sodium hypochlorite under several formulations on root canal dentin microhardness

2013 ◽  
Vol 4 (4) ◽  
pp. 229-232 ◽  
Author(s):  
Arturo J.A. Garcia ◽  
Milton C. Kuga ◽  
Regina G. Palma-Dibb ◽  
Marcus V.R. Só ◽  
Mariza A. Matsumoto ◽  
...  
Keyword(s):  
Sodium Hypochlorite ◽  
Root Canal ◽  
Root Canal Dentin

scholarly journals Evaluation of the Influence of the Nd:YAG Laser and Different Irrigants on the Bond Strength of the Adhesion of the Fiber Posts to Root Dentin using a Self-etching Adhesive System

2013 ◽  
Vol 4 (3) ◽  
pp. 170-174
Author(s):  
Cesar Rogério Pucci ◽  
Carlos Rocha Gomes Torres ◽  
Cláudio Antonio Talge Carvalho ◽  
Ana Julia Farias Lacerda ◽  
Marina Gullo ◽  
...  
Keyword(s):  
Bond Strength ◽  
Sodium Hypochlorite ◽  
Nd:Yag Laser ◽  
Root Canal ◽  
Adhesive System ◽  
Root Canal Filling ◽  
Yag Laser ◽  
Fiber Posts ◽  
Significant Difference ◽  
Root Dentin

ABSTRACT Aim The aim of this study was to investigate the effects of pretreatment of different irrigating solutions with and without the application the Nd: YAG laser on the bond strength (RU) of the adhesion of the fiberglass post relined with resin composite to the root dentin. Materials and methods Sixty-six single bovine incisor roots were divided into 6 groups according to irrigating solution and with/without the apllication of the Nd: YAG laser after root canal filling: S- saline; C-2% chlorhexidine solution, H-2.5% sodium hypochlorite; SL-saline + laser, CL-2% chlorhexidine + laser; HL-2.5% sodium hypochlorite + laser (all n = 11). The roots were standardized up to a file # 80 and root-filled with gutta-percha (Dentsply) and Sealer 26 (Dentsply) by lateral condensation. The root canal filling was partly removed. Afterward, root canals were irrigated with 5 ml of the selected irrigant and the fiberglass post were cemented with epoxy resin curing dual Bifix QM (VOCO) and a self-etch adhesive system Futurabond DC (VOCO). All roots were sectioned transversely, and the push-out tested was performed. The bond strength means were analyzed by the analysis of variance and Tukey test (α= 5%). Results The following bond strength MPa values—were recorded for each group: group C—7.77(±1.8)a, LC—6.46 (±2.2)a, S7.33 (±2.2)a, LS 7.35 (±1.68)a, H 5.43 (±1,3)b, LH 5.13 (±1,6)b. A signifcant bond strenght decrease was noticed with the use of 2.5% NaOCl (p < 0.05). No statistical significant difference were found with our without the apllication of the Nd:YAG laser (p > 0.05). Conclusion Regardless the irrigant tested, the use of Nd: YAG laser can be indicated prior to cementation of fiberglass without loss in bond strength. How to cite this article Pucci CR, Lacerda AJF, Gullo M, Xavier ACC, Torres CRG, Martinho FC, Carvalho CAT. Evaluation of the Influence of the Nd:YAG Laser and Different Irrigants on the Bond Strength of the Adhesion of the Fiber Posts to Root Dentin using a Self-etching Adhesive System. World J Dent 2013;4(3):170-174.

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