scholarly journals Peri-implant health after supportive mucositis therapy is associated with increased levels of FGF-2

2021 ◽  
Vol 32 (5) ◽  
pp. 55-66
Author(s):  
Juliana Prazeres Gonçalves de Castro ◽  
Telma Regina da Silva Aguiar ◽  
Gilson Coutinho Tristão ◽  
Gutemberg Gomes Alves ◽  
Marina Prado Fernandes Pinheiro ◽  
...  
Keyword(s):  
Supportive Therapy ◽  
Control Group ◽  
Clear Correlation ◽  
Non Invasive ◽  
Significant Difference ◽  
Prosthesis Removal ◽  
Implant Therapy ◽  
Crevicular Fluid ◽  
Keratinized Mucosa ◽  
Therapy Protocol

Abstract This study aimed to analyze Fibroblast Growth Factor-2 (FGF-2) levels in the peri-implant crevicular fluid throughout supportive mucositis therapy. Twenty-six participants with Branemark protocol prosthesis were divided into two groups: the control group, characterized by healthy peri-implants, and the mucositis group, presenting a diagnosis of peri-implant mucositis. All participants underwent clinical examination, radiographic analysis, prosthesis removal, and non-invasive peri-implant therapy (mechanical debridement associated with chlorhexidine 0.12%) during a period of 36 days divided into three intervals. Peri-implant crevicular fluid samples were collected at each interval in order to analyze FGF-2 levels by immuno-enzymatic assay. The control and mucositis groups showed difference in keratinized mucosa. The smaller the range of keratinized mucosa the higher susceptibility of peri-implant mucositis. Throughout the treatment intervals, participants were diagnosed in different groups indicating whether or not the non-invasive therapy was able to treat peri-implant mucositis. There was a significant difference of FGF-2 levels between groups, with the higher FGF-2 levels in the control group (p=0.01). After supportive therapy, the mucositis group showed significantly increased FGF-2 levels (p<0.01) compared to initial levels. After 36 days of supportive therapy, there was a reduction of peri-implant mucositis from 70% to 23%. Clinical and laboratory outcomes showed a clear correlation since FGF-2 levels increased after 36 days. It was concluded that the therapy protocol was effective and promoted a regenerative reaction and FGF-2 can be considered a future target for peri-implant mucositis understanding.

scholarly journals THE EFFECTS OF PANORAMIC RADIOGRAPHY ON GINGIVAL CREVICULAR FLUID VOLUME IN GINGIVITIS

2021 ◽  
Vol 6 (2) ◽  
pp. 136
Author(s):  
Rini Rahmiyati ◽  
Didit Aspriyanto ◽  
Beta Widya Oktiani
Keyword(s):  
Filter Paper ◽  
Wistar Rats ◽  
Gingival Crevicular Fluid ◽  
Panoramic Radiography ◽  
Cell Damage ◽  
Biological Fluid ◽  
Control Group ◽  
Control Group Design ◽  
Significant Difference ◽  
Crevicular Fluid

Background: Gingival crevicular fluid (GCF) is a biological fluid derived from the gingival sulcus and can be elevated in the inflammatory state of periodontal tissue, such as gingivitis. In previous studies, the number of GCF could also increase after panoramic radiographic exposure. Increase in GCF due to panoramic radiography is a sign of cell damage. Objective: To analyze the effects of panoramic radiography on the volume of GCF in wistar rats with gingivitis. Method: This type of research was true experimental with post test only and control group design. The sampling technique used was simple random sampling. A total of 25 wistar rats were classified into two control groups without exposure (state without gingivitis and gingivitis) and three groups treatment of exposure (state of gingivitis with 1 time, 2 times, and 3 times the exposure). GCF sampling using filter paper was carried out 10 minutes after panoramic radiographic exposure. The filter paper was stained by 2% ninhydrin solution, after that the GCF volume was calculated. Results: There was a significant difference in the number of GCF (p<0.05) in the group without gingivitis and exposure compared to all other groups, the gingivitis group without exposure compared to the gingivitis group with 3 times exposure, and the gingivitis group with 1 time exposure compared to the gingivitis group 3 times with exposure. Conclusion: Panoramic radiography can cause an increase in the volume of GCF in wistar rats with gingivitis. Keywords: GCF, gingivitis, panoramic radiography

scholarly journals Efficiency of Nicotinamide-based Supportive Therapy in Lymphopenia for Patients With COVID-19: A Randomized Controlled Trial

2022 ◽  
Author(s):  
Qiang Hu ◽  
Quan-Yu Zhang ◽  
Cheng-Fei Peng ◽  
Zhuang Ma ◽  
Ya-Ling Han
Keyword(s):  
Controlled Trial ◽  
Supportive Therapy ◽  
Composite Endpoint ◽  
Hospital Death ◽  
Control Group ◽  
Improvement Rate ◽  
Significant Difference ◽  
Before And After ◽  
Secondary Endpoints ◽  
Absolute Lymphocyte Counts

Abstract Background: The purpose of this study was to investigate the efficiency of nicotinamide-based supportive therapy in lymphopenia for patients with coronavirus disease-2019 (COVID-19). Methods: 24 patients diagnosed with the COVID-19 were randomly divided into two groups (n=12) during hospitalization in the ratio of 1:1. Based on the conventional treatment, the treatment group was given 100mg nicotinamide, five times a day. The control group only received routine treatments. The primary endpoint was the change in absolute lymphocyte counts. The secondary endpoints included both the in-hospital death and the composite endpoint of aggravation, according to upgraded oxygen therapy, improvement of nursing level, and ward rounds of superior physicians for changes of conditions. Results: The full blood counts before and after receiving the nicotinamide were comparable in each group (all P>0.05). Before and after receiving the nicotinamide, mean absolute lymphocyte counts were similar between the two groups ([0.94±0.26]*109/L versus [0.89±0.19]*109/L, P=0.565; [1.15±0.48]*109/L versus [1.02±0.28]*109/L, P=0.445, respectively). Therefore, there was no statistically significant difference in the lymphocyte improvement rate between the two groups (23.08±46.10 versus 16.52±24.10, P=0.67). There was also no statistically significant difference for the secondary endpoints between the two groups.Conclusion: Among patients with COVID-19, there was no statistically significant difference in change of full blood counts and the absolute lymphocyte counts before and after intervention in both groups. Therefore, no new evidence was found for the effect of niacinamide on lymphopenia in patients with COVID-19.Trial registration: ClinicalTrials.gov, NCT04910230. Registered 1 June 2021-retrospectively registered.

Efficacy of subgingivally delivered atorvastatin and simvastatin as an adjunct to scaling and root planing

2015 ◽  
Vol 30 (4) ◽  
Author(s):  
Sai M. Surve ◽  
Anirudh B. Acharya ◽  
Srinath L. Thakur
Keyword(s):  
Chronic Periodontitis ◽  
Gingival Crevicular Fluid ◽  
Local Delivery ◽  
Control Group ◽  
Clinical Parameters ◽  
Scaling And Root Planing ◽  
Root Planing ◽  
Significant Difference ◽  
Group 2 ◽  
Crevicular Fluid

AbstractThe current understanding of the pathogenesis of periodontal disease has resulted in adjunctive use of various pharmacologic agents in periodontal therapy. The objective of this investigation was to assess the efficacy of atorvastatin and simvastatin (because of their pleiotropic properties) as an adjunct to dental scaling and root planing (SRP) by local delivery, i.e. placing them subgingivally, in the treatment of chronic periodontitis.Local delivery systems for atorvastatin and simvastatin were prepared in sodium alginate suspension to be administered with calcium chloride solution. Patients diagnosed with chronic periodontitis were grouped as group 1, receiving SRP only (control), group 2, receiving SRP with subgingival delivery of 1.2% simvastatin, and group 3, receiving SRP with subgingival delivery of 1.2% atorvastatin. Clinical parameters and interleukin (IL) 1α levels in the gingival crevicular fluid (GCF) were assessed.All three groups showed significant reductions in clinical parameters and IL-1α levels in the GCF (p<0.05). However, the test groups did not show any statistically significant difference when compared with control.Subgingivally delivered atorvastatin and simvastatin as an adjunct to SRP is efficacious but did not demonstrate any added benefit as compared with SRP alone.

scholarly journals Comparison of Perineal Sonographically Measured and Functional Urodynamic Urethral Length in Female Urinary Incontinence

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Laila Najjari ◽  
Nadine Janetzki ◽  
Lieven Kennes ◽  
Elmar Stickeler ◽  
Julia Serno ◽  
...  
Keyword(s):  
Urinary Incontinence ◽  
Patient Group ◽  
Pairwise Comparison ◽  
Control Group ◽  
Clear Correlation ◽  
Perineal Ultrasound ◽  
Female Urinary Incontinence ◽  
Mixed Incontinence ◽  
Suitable Parameter ◽  
Significant Difference

Objectives. To detect the anatomical insufficiency of the urethra and to propose perineal ultrasound as a useful, noninvasive tool for the evaluation of incontinence, we compared the anatomical length of the urethra with the urodynamic functional urethral length. We also compared the urethral length between continent and incontinent females.Methods. 149 female patients were enrolled and divided into four groups (stress, urge, or mixed incontinence; control). Sonographically measured urethral length (SUL) and urodynamic functional urethral length (FUL) were analyzed statistically. Standardized and internationally validated incontinence questionnaire ICIQ-SF results were compared between each patient group.Results. Perineal SUL was significantly longer in incontinent compared to continent patients (p<0.0001). Pairwise comparison of each incontinent type (stress, urge, or mixed incontinence) with the control group showed also a significant difference (p<0.05). FUL was significantly shorter in incontinent patients than in the control group (p=0.0112). But pairwise comparison showed only a significant difference for the stress incontinence group compared with the control group (p=0.0084) and not for the urge or mixed incontinent group. No clear correlation between SUL, FUL, and ICIQ-SF score was found.Conclusions. SUL measured by noninvasive perineal ultrasound is a suitable parameter in the assessment of female incontinence, since incontinent women show a significantly elongated urethra as a sign of tissue insufficiency, independent of the type of incontinence.

scholarly journals Is There a Balance in Oxidative-Antioxidant Status in Blood Serum of Patients with Advanced Endometriosis?

Antioxidants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1097
Author(s):  
Izabela Kokot ◽  
Agnieszka Piwowar ◽  
Marcin Jędryka ◽  
Ewa Maria Kratz
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Status ◽  
Total Bilirubin ◽  
Redox Homeostasis ◽  
Curve Analysis ◽  
Control Group ◽  
Roc Curve Analysis ◽  
Non Invasive ◽  
Gynecological Disorders ◽  
Significant Difference

Can redox homeostasis indicators be potential non-invasive markers, crucial in the diagnosis and treatment of endometriosis? We checked if the differences in levels of serum oxidative-antioxidant balance parameters (TAS, FRAP, albumin, total bilirubin, uric acid, iron, SIRT3, SIRT5, SIRT6, telomerase, AOPP) are significant between patients with advanced endometriosis (E), healthy women (control group, C) and non-endometriosis women, but with other gynecological disorders (NE). The FRAP concentrations were significantly higher in E and NE group than in the control group (p = 0.015 and p = 0.017, respectively). The telomerase concentrations were significantly higher in the endometriosis group than in the control group (p = 0.004). Significantly higher concentrations of AOPP were observed in E (p < 0.001) and NE groups (p = 0.028) in comparison to the control subjects. Between stages III and IV of endometriosis, a significant difference existed only in concentration of iron (p = 0.013). There were no significant differences between the studied groups in the values of the remaining parameters. Based on the results of ROC curve analysis, we can conclude that the levels of serum FRAP, telomerase and AOPP may be taken into account as promising diagnostics markers that reflect the degree of oxidative stress accompanying advanced endometriosis.

scholarly journals Evaluation of Plasma MMP-9 and VEGF-A mRNAs as Non-invasive Diagnostic Biomarkers in Women with Endometriosis

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Sepideh Abdollahi ◽  
Pantea Izadi ◽  
Shahla Noori Ardebili ◽  
Samaneh Chegeni ◽  
Mir Saead Yekaninejad
Keyword(s):  
Plasma Level ◽  
Quantitative Polymerase Chain Reaction ◽  
Mrna Level ◽  
Control Group ◽  
P Value ◽  
Mrna Levels ◽  
Diagnostic Biomarker ◽  
Diagnostic Biomarkers ◽  
Non Invasive ◽  
Significant Difference

Background: Endometriosis is one of the common gynecological diseases and can lead to pelvic pain, dysmenorrhea, dyspareunia, and infertility in women. Thus, accurate and early diagnosis is a pivotal issue and an essential need for managing this disorder. At the present, the gold standard diagnostic method for endometriosis is laparoscopic surgery that is an invasive method and can lead to delay in diagnosis. Thus, there is an immediate necessity to search for non-invasive diagnostic biomarkers, such as blood-based ones. Objectives: Matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor-A (VEGF-A) have essential roles in the pathogenesis of endometriosis. Therefore, in this study, we evaluated the plasma mRNA levels of MMP-9 and VEGF-A, as potential non-invasive diagnostic biomarkers for endometriosis. Methods: This study included 48 women (24 cases and 24 controls) who underwent laparoscopy for suspected endometriosis. Preoperative plasma samples were collected, and after RNA extraction, the levels of MMP-9 and VEGF-A mRNAs were determined by reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Results: Plasma MMP-9 mRNA level was statistically higher in endometriosis patients compared with the control group (P value = 0.01). However, plasma VEGF-A mRNA level did not show a significant difference between the two groups (P value =0.5). Conclusions: It seems that the plasma level of MMP-9 mRNA in endometriosis patients is significantly higher than in non-endometriosis women. This finding can provide new insights regarding this mRNA’s applicability as a non-invasive diagnostic biomarker for discovering new cases of endometriosis (newly diagnosed). According to our results, despite the suggested role of VEGF-A in endometriosis pathogenesis, it seems that the plasma level of VEGF-A mRNA does not have the potential to be used as a non-invasive diagnostic biomarker.

scholarly journals Quantification of Sphingosine-1-Phosphate (S1P) in Patients with Periodontitis Stage II Grade B

2021 ◽  
pp. 413-421
Author(s):  
María I. García-Parra ◽  
Matilde Jiménez-Coello ◽  
Bertha A. Carrillo-Ávila ◽  
Eduardo A. Sauri Esquivel ◽  
Víctor M. Martínez-Aguilar ◽  
...  
Keyword(s):  
Case Control Study ◽  
Gingival Crevicular Fluid ◽  
Periodontal Diseases ◽  
Control Group ◽  
Future Studies ◽  
Elisa Kit ◽  
Significant Difference ◽  
Sphingosine 1 Phosphate ◽  
Crevicular Fluid ◽  
Control Study

The aim of this study was the quantification of Sphingosine-1-phosphate (S1P) in periodontal pockets of patients with periodontitis. This is an observational, descriptive, case-control study. Thirty subjects were selected: 15 controls and 15 cases. A periodontal study was conducted following the parameters of AAP 2017 for the diagnosis of periodontal diseases. A sample of saliva and gingival crevicular fluid was obtained from each subject and then analyzed with the Human S1P Elisa kit (MyBioSource #MBS2516132) accordingly to the manufacturer's instructions, in order to verify the presence of S1P and quantify it´s concentration when founded. Results showed a significant difference (p=0.05) between cases and controls. In the case of saliva samples, the concentration of S1P was higher than the ones found in the control group (72.94 ng/mL and 45.12 ng/mL). For GCF, a higher amount of S1P was found in patients with POD (20.09 ng/mL and 15.20 ng/mL). This work raises a possible route of bone metabolism, inflammatory process, and identification of periodontitis through oral quantification of S1P, however, future studies are needed.

scholarly journals SERUM MYELOPEROXIDASE, MALONDIALDEHYDE, ALPHA-SYNUCLEIN LEVELS IN PATIENTS WITH EPILEPSY

2021 ◽  
Vol 7 (2) ◽  
pp. 93-97
Author(s):  
Fatma Şimşek ◽  
Mustafa Ceylan ◽  
Seda Aşkın ◽  
Ahmet Kızıltunç
Keyword(s):  
Disease Duration ◽  
Alpha Synuclein ◽  
Control Group ◽  
Barrier Dysfunction ◽  
Serum Samples ◽  
Non Invasive ◽  
Suitable Parameter ◽  
Significant Difference ◽  
Patients With Epilepsy

Background: Processes such as neurodegeneration, hypoxia, blood brain barrier dysfunction and oxidative changes are effective for epileptogenesis.There is no non-invasive biomarker that can be used in the follow-up of patients with epilepsy, which is a neurodegenerative disease. Objective: In our study, it was aimed to investigate the relationship between inflammatory, oxidative, neurodegenerative processes, and antiepileptic use in patients with epilepsy. Methods: The groups were formed from the patients who were followed up in the epilepsy outpatient clinic between April 2019-June 2019, and the age-gender-matched control group.The study included 30 patients and 30 healthy volunteers. Venous serum samples were collected from groups to study myeloperoxidase, malondialdehyde and alpha-synuclein. Results: The levels of myeloperoxidase and malondialdehyde were higher in the control group and this difference was statistically significant (p=0.003, p<0.001). The level of α-syn was higher in the epilepsy group and there was no statistically significant difference between the two groups (p=0.52). There was a positive correlation between the α-syn level and disease duration and as the disease duration increased, the level of α-syn increased (r=0.379, p=0.03). Conclusion: Although the α-syn level increases with the duration of the disease in epilepsy patients, it is not a suitable parameter for use as a biomarker in the follow-up.

Efficiency and safety of terlipressin application during caesarian section in pregnant women with a high risk of bleeding: a multicenter comprehensive cohort study of Terli-Bleed. Part II

2021 ◽  
Vol 20 (2) ◽  
pp. 26-32
Author(s):  
Yu.S. Raspopin ◽  
◽  
E.M. Shifman ◽  
A.A. Belinina ◽  
A.V. Rostovtsev ◽  
...  
Keyword(s):  
High Risk ◽  
Caesarean Section ◽  
Pregnant Women ◽  
Postpartum Haemorrhage ◽  
Research Work ◽  
Control Group ◽  
Non Invasive ◽  
Group I ◽  
Significant Difference ◽  
Invasive Hemodynamics

Severe and massive bleeding remains one of the main causes of maternal mortality and morbidity. The use of terlipressin has proved to be effective in the prevention of postpartum haemorrhage in the high-risk group. Given that terlipressin is a potent vasopressor, there are concerns about its use in parturient women with hypertension. Objective. To evaluate the hemodynamic effects of terlipressin when it is injected into the myometrium during caesarean section. Patients and methods. This publication is a continuation of the research work on the effectiveness of terlipressin in the prevention of postpartum haemorrhage in high-risk pregnant women, which was conducted between February and December 2020 with the participation of 5 medical centers. The study included 454 pregnant women who delivered by caesarean section. They were divided into two groups: control group I (n = 351) and study group II (n = 103), with local application of terlipressin injected into the thickness of the myometrium. During the study, the parameters of non-invasive hemodynamics were assessed. Results. When assessing the indicators of non-invasive blood pressure, no statistically significant difference was found between the groups. The analysis of heart rate showed slight statistical difference at the stage of operation and in the postoperative period. A significant difference in values of shock index in dynamics was found between the groups, which, nevertheless, were within acceptable limits, and the difference had no clinical significance. Conclusion. The study showed that the use of terlipressin does not significantly affect the parameters of non-invasive hemodynamics, which can complicate the operation or the labor outcome. Key words: obstetric haemorrhage, terlipressin, hemodynamics

scholarly journals Assessment of portal venous index as a non-invasive method for diagnosing liver fibrosis in patients with chronic hepatitis C

2012 ◽  
Vol 49 (1) ◽  
pp. 14-18 ◽  
Author(s):  
Haroldo Luis Oliva Gomes Rocha ◽  
Angélica Lemos Debs Diniz ◽  
Valéria Ferreira de Almeida e Borges ◽  
Frederico Chaves Salomão
Keyword(s):  
Chronic Hepatitis ◽  
Hepatitis C ◽  
Liver Biopsy ◽  
Portal Vein ◽  
Chronic Hepatitis C ◽  
Hepatic Fibrosis ◽  
Control Group ◽  
Non Invasive ◽  
Significant Difference ◽  
Spearman Test

CONTEXT: Hepatitis C is an important cause of chronic liver disease worldwide. The grading of hepatic fibrosis in chronic hepatitis C is important for better clinical management. However, until now, liver biopsy is the only test accepted for this purpose, despite their contraindications and complications. New methods for non-invasive assessment of hepatic fibrosis are under investigation. One proposal is the Doppler ultrasound, as a non-invasive, widely available and inexpensive. OBJECTIVE: To compare Doppler parameters of portal vein in patients with chronic hepatitis C with a healthy control group and to correlate these parameters with fibrosis degree obtained by liver biopsy. METHODS: Fifty patients with chronic hepatitis C submitted to liver biopsy and 44 healthy controls had Doppler of the portal vein performed, with the calculation of the portal venous index. We conducted a comparison between the averages of the two groups of portal venous index. For the correlation between portal venous index and fibrosis was employed the Spearman test. RESULTS: There was a difference between the average portal venous index between controls (0.33 ± 0.07) and patients (0.23 ± 0.09) with P<0.001. No difference was observed between the portal venous index in patients with chronic hepatitis C who have significant fibrosis or not. The correlation between the portal venous index and fibrosis degree was reverse and moderate (r =-0.448 P<0.001). The area under the ROC curve was 78.4% (95% CI: 68.8% to 88%). The cutoff for the portal venous index was 0.28 with sensitivity of 73.5% and specificity of 71.1%. CONCLUSION: The portal venous index was useful in distinguishing healthy patients from patients with CHC. However, there was no significant difference in the quantification of degree of fibrosis.

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