Quantification of Sphingosine-1-Phosphate (S1P) in Patients with Periodontitis Stage II Grade B

The aim of this study was the quantification of Sphingosine-1-phosphate (S1P) in periodontal pockets of patients with periodontitis. This is an observational, descriptive, case-control study. Thirty subjects were selected: 15 controls and 15 cases. A periodontal study was conducted following the parameters of AAP 2017 for the diagnosis of periodontal diseases. A sample of saliva and gingival crevicular fluid was obtained from each subject and then analyzed with the Human S1P Elisa kit (MyBioSource #MBS2516132) accordingly to the manufacturer's instructions, in order to verify the presence of S1P and quantify it´s concentration when founded. Results showed a significant difference (p=0.05) between cases and controls. In the case of saliva samples, the concentration of S1P was higher than the ones found in the control group (72.94 ng/mL and 45.12 ng/mL). For GCF, a higher amount of S1P was found in patients with POD (20.09 ng/mL and 15.20 ng/mL). This work raises a possible route of bone metabolism, inflammatory process, and identification of periodontitis through oral quantification of S1P, however, future studies are needed.

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Are Proteinase 3 and Cathepsin C Enzymes Related to Pathogenesis of Periodontitis?

BioMed Research International ◽

10.1155/2014/420830 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 2

Author(s):

Oya Türkoğlu ◽

Elif Azarsız ◽

Gülnur Emingil ◽

Necil Kütükçüler ◽

Gül Atilla

Keyword(s):

Gingival Crevicular Fluid ◽

Periodontal Diseases ◽

Study Groups ◽

Aggressive Periodontitis ◽

Control Group ◽

Proteinase 3 ◽

Cathepsin C ◽

Probing Depth ◽

Inflammatory Processes ◽

Crevicular Fluid

Aim. Cathepsin C is the activator of the polymorphonuclear leukocyte-derived proteinase 3, which contributes to inflammatory processes. The aim of the present study was to investigate gingival crevicular fluid (GCF) proteinase 3 and cathepsin C levels in periodontal diseases.Design. Eighteen patients with chronic periodontitis (CP), 20 patients with generalized aggressive periodontitis (G-AgP), 20 patients with gingivitis, and 18 healthy subjects were included in the study. Periodontal parameters including probing depth, clinical attachment level, papilla bleeding index, and plaque index were assessed in all study subjects. GCF proteinase 3 and cathepsin C levels were analyzed by ELISA.Results. GCF proteinase 3 total amount was significantly higher in diseased groups compared to control group, after adjusting ageP<0.05. No differences were found in GCF cathepsin C levels among the study groupsP>0.05. Periodontal parameters of sampling sites were positively correlated with GCF proteinase 3 total amountsP<0.01but not with cathepsin C total amountsP>0.05.Conclusions. Elevated levels of GCF proteinase 3 in CP, G-AgP, and gingivitis might suggest that proteinase 3 plays a role during inflammatory periodontal events in host response. However, cathepsin C in GCF does not seem to have an effect on the pathogenesis of periodontal diseases.

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The association of male infertility with telomere length: A case control study

Indian Journal of Clinical Anatomy and Physiology ◽

10.18231/j.ijcap.2021.070 ◽

2021 ◽

Vol 8 (4) ◽

pp. 325-332

Author(s):

Kate Deepali Rajesh ◽

Puranam Vatsalaswamy ◽

Manvikar Purshotam Rao

Keyword(s):

Male Infertility ◽

Telomere Length ◽

Real Time ◽

Real Time Pcr ◽

Case Control Study ◽

Case Control ◽

Control Group ◽

Significant Difference ◽

Control Study ◽

Sperm Telomere Length

To study the relevance of sperm telomere length and infertility in men. : Our case-control study included twenty-five males in couple with sub-fertility/infertility (test group) and twenty five healthy males (control group) with proven paternity in the age group 25 to 35 years. The Absolute Sperm Telomere length (aSTL) was measured by real-time PCR. We investigated whether any significant difference in the aSTL value existed between the groups and analysed the relationship between aSTL and other sperm parameters.The mean (SE) aSTL recorded in the infertile cases was significantly shorter than for the control group being 140.60 (6.66) Kb/genome and 239.63 (12.32) Kb/genome respectively (p <0.001) A weak correlation was eminent between aSTL kb/genome and the total sperm count mil/ml (rho= 0.04, p - 0.86), progressive sperm motility (rho= - 0.02, p=0.934) and sperm viability (rho= - 0.07 p=0.741) in the infertile group. The measurement of aSTL by real-time PCR is a simple and rapid method that offers further paramount information with respective to the quality of sperm. It is befitted for epidemiological studies, hence opening new perspectives in the evaluation of male infertility. Limitations - Our study was confined to men aged between 25 and 35 years. Further comparative studies are needed to explore the significance of STL and infertility in older males. Additional studies will help illumine the significance of aSTL as a prognostic biomarker in assisted reproduction.

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The Relationship between Serum Levels of Vitamin C, Uric Acid and Antioxidant Condition with Coronary Artery Diseases: a Case-Control Study

10.21203/rs.2.15439/v1 ◽

2019 ◽

Author(s):

Aida Torkzaban ◽

Seyed Amir Mansour Alavi Naeini ◽

Akbar Hassanzadeh ◽

Mehrdad Namdari

Keyword(s):

Uric Acid ◽

Vitamin C ◽

Case Control Study ◽

Demographic Data ◽

Systolic Pressure ◽

Case Control ◽

Control Group ◽

Case Group ◽

Significant Difference ◽

Control Study

Abstract Background Coronary hearth diseases are among the main causes of death in adults. Increase of oxidative stress and defects in antioxidant defense play a major role in endothelium performance and are an effective factor in progress of atherosclerosis. Some studies have also reported different malondialdehyde and total antioxidant capacity among the atherosclerosis patients.Methods In this case-control study, 44 atherosclerosis patients referring to Shahid Madani treatment-education center were considered as the case group; while 44 healthy peoples were placed in the control group. Demographic data and anthropometric indices were measured. Food frequency questionnaire and international physical activity questionnaires were also completed. After 12 hours of fasting, 10 ml blood was sampled from the participants. Uric acid, vitamin C, TAC and MDA were also measured. The data were finally analyzed by SPSS Ver 22 software.Results A significant difference was observed between the two groups in terms of uric acid (P<0.001) and vitamin C (P<0.03). However, mean MDA and TAC showed no significant difference between the two groups. The two groups’ difference in terms of vitamin A, E and beta carotene, zinc and selenium intake was not significant. A significant difference was however detected between the two groups in terms of vitamin C (P<0.047). A significant relationship was also observed between the systolic pressure and CHD (P<0.028).Conclusion Results of this study indicated that the uric acid and vitamin C levels of atherosclerosis patients had significant increase and decrease in comparison with the healthy subjects, respectively. Mean TAC and antioxidant levels of their diets (except for vitamin C) showed no significant difference. Systolic blood pressure of the patients was significantly higher than the controls.

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THE EFFECTS OF PANORAMIC RADIOGRAPHY ON GINGIVAL CREVICULAR FLUID VOLUME IN GINGIVITIS

Dentino : Jurnal Kedokteran Gigi ◽

10.20527/dentino.v6i2.11995 ◽

2021 ◽

Vol 6 (2) ◽

pp. 136

Author(s):

Rini Rahmiyati ◽

Didit Aspriyanto ◽

Beta Widya Oktiani

Keyword(s):

Filter Paper ◽

Wistar Rats ◽

Gingival Crevicular Fluid ◽

Panoramic Radiography ◽

Cell Damage ◽

Biological Fluid ◽

Control Group ◽

Control Group Design ◽

Significant Difference ◽

Crevicular Fluid

Background: Gingival crevicular fluid (GCF) is a biological fluid derived from the gingival sulcus and can be elevated in the inflammatory state of periodontal tissue, such as gingivitis. In previous studies, the number of GCF could also increase after panoramic radiographic exposure. Increase in GCF due to panoramic radiography is a sign of cell damage. Objective: To analyze the effects of panoramic radiography on the volume of GCF in wistar rats with gingivitis. Method: This type of research was true experimental with post test only and control group design. The sampling technique used was simple random sampling. A total of 25 wistar rats were classified into two control groups without exposure (state without gingivitis and gingivitis) and three groups treatment of exposure (state of gingivitis with 1 time, 2 times, and 3 times the exposure). GCF sampling using filter paper was carried out 10 minutes after panoramic radiographic exposure. The filter paper was stained by 2% ninhydrin solution, after that the GCF volume was calculated. Results: There was a significant difference in the number of GCF (p<0.05) in the group without gingivitis and exposure compared to all other groups, the gingivitis group without exposure compared to the gingivitis group with 3 times exposure, and the gingivitis group with 1 time exposure compared to the gingivitis group 3 times with exposure. Conclusion: Panoramic radiography can cause an increase in the volume of GCF in wistar rats with gingivitis. Keywords: GCF, gingivitis, panoramic radiography

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Interaction between serum FGF-23 and PTH in renal phosphate excretion, a case-control study in hypoparathyroid patients

10.21203/rs.2.12237/v2 ◽

2019 ◽

Author(s):

Forough Saki ◽

Seyed Reza Kassaee ◽

Azita Salehifar Salehifar ◽

gholamhossein Ranjbar omrani

Keyword(s):

Parathyroid Hormone ◽

Serum Calcium ◽

Case Control Study ◽

Fractional Excretion ◽

Case Control ◽

Control Group ◽

Phosphate Excretion ◽

Significant Difference ◽

Fgf 23 ◽

Control Study

Abstract Background:phosphate homeostasis is mediated through complex counter regulatory feed-back balance between parathyroid hormone, FGF-23 and 1,25(OH)2D. Both parathyroid hormone and FGF-23 regulate proximal tubular phosphate excretion through signaling on sodium- phosphate cotransporters II a and II c . However, the interaction between these hormones on phosphate excretion is not clearly understood. We performed the present study to evaluate whether the existence of sufficient parathyroid hormone is necessary for full phosphaturic function of FGF-23 or not. Methods:In this case-control study, 19 patients with hypoparathyroidism and their age- and gender-matched normal population were enrolled. Serum calcium, phosphate, alkaline phosphatase,parathyroid hormone, FGF-23, 25(OH)D, 1,25(OH)2D and Fractional excretion of phosphorous were assessed and compared between the two groups, using SPSS software. Results:The mean serum calcium and parathyroid hormone level was significantly lower in hypoparathyroid patients in comparison with the control group(P<0.001 and P<0.001, respectively). We found high serum level of phosphate and FGF-23 in hypoparathyroid patients compared to the control group (P<0.001 and P<0.001,respectively). However, there was no significant difference in Fractional excretion of phosphorous or 1,25OH2D level between the two groups. There was a positive correlation between serum FGF-23 and Fractional excretion of phosphorous just in the normal individuals (P <0.001, r = 0.79). Conclusions:Although the FGF-23 is a main regulator of urinary phosphate excretion but the existence of sufficient parathyroid hormone is necessary for the full phosphaturic effect of FGF-23.

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The Effect of Crystal Dependence on Brain Activity Related to the Perception of Pleasure Using fMRI

Archives of Neuroscience ◽

10.5812/ans.100907 ◽

2021 ◽

Vol 8 (3) ◽

Author(s):

Ali Yoonessi ◽

Seyed Amir Hossein Batouli ◽

Iman Ahmadnezhad ◽

Hamid Soltanian-zadeh

Keyword(s):

Case Control Study ◽

Brain Activity ◽

Economic Status ◽

Smoking History ◽

Control Group ◽

Human Society ◽

Significant Difference ◽

History Of ◽

The Brain ◽

Control Study

Background: Addiction is currently one of the problems of human society. Drug abuse is one of the most important issues in the field of addiction. Methamphetamine (crystal) is one of the drugs that has been abused in recent decades. Methods: In this case-control study, 10 individuals aged 20 to 40 years old with at least 2 years of experience of methamphetamine consumption without any history of drug use or other stimulants from clients and drug withdrawal centers in Tehran City, and 10 healthy volunteers were selected. Age, social status, and economic status of addicts were included in the fMRI apparatus, and 90 selected pleasurable, non-pleasurable, and neutral images (IAPS) were displayed by the projector through an event-related method. The playback time of each photo was 3 s, and after this process, the person outside the device, without the time limit selected the enjoyable and unpleasant images. Results: The results showed that there was no significant difference between the groups in terms of age, alcohol use, and smoking history (P < 0.05). There was no significant difference in terms of the age at first use between members of the methamphetamine-dependent group. Also, the methamphetamine-dependent group showed more brain activity in their pre-center and post-center gyrus than the normal (control) group. Conclusions: According to the results obtained in this study, in general, it can be concluded that there are some areas in the brain of addicts that are activated when watching pleasant photos, while these areas are not active in the brains of normal people.

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Is There a Relationship between Patellofemoral Alignment and Osgood–Schlatter Disease? A Case-Control Study

The Journal of Knee Surgery ◽

10.1055/s-0038-1676523 ◽

2018 ◽

Vol 33 (01) ◽

pp. 067-072 ◽

Cited By ~ 2

Author(s):

Fırat Seyfettinoğlu ◽

Özkan Köse ◽

Hasan Ulaş Oğur ◽

Ümit Tuhanioğlu ◽

Hakan Çiçek ◽

...

Keyword(s):

Case Control Study ◽

Case Control ◽

Radiographic Examination ◽

Control Group ◽

Etiologic Factor ◽

Full Extension ◽

Significant Difference ◽

Patellofemoral Alignment ◽

The Difference ◽

Control Study

AbstractThis study was aimed to investigate the role of anatomic variations in patellofemoral alignment in patients with or without Osgood-Schlatter disease (OSD) and to determine the potential anatomic risk factors that may play role in the etiology. This prospective observational case-control study was conducted on two groups of adolescent patients. Group 1 comprised patients who were diagnosed as having OSD and group 2 consisted of an equal number of age-matched patients who presented to the outpatient clinic with traumatic knee injury and underwent knee radiographic examination but without a diagnosis of OSD. Age, height, weight, body mass index (BMI), dominant side, and level of sporting activity were recorded. Quadriceps (Q) angles were measured using a long-arm goniometer with patients lying in the supine position with their knees in full extension and contracting the Q muscles. On anteroposterior, lateral knee, and tangential patella (Merchant and Laurin views) radiographs, the following measurements were performed: Insall–Salvati (IS), Caton–Deschamps (CD), and Blackburne–Peel (BP) indexes, congruence angles, lateral patellofemoral angles, sulcus angles, and patella type according to Grelsamer's morphology classification. Both groups were similar in respect of age (p = 0.160), sex (p = 0.311), height (p = 0.326), weight (p = 0.596), BMI (p = 0.153), and dominancy (p = 0.500). The rate of patients engaged in sports activities was significantly greater in the OSD group (p = 0.003). No significant difference was determined between the groups in respect to IS index, CD index, BP index, sulcus angle, lateral patellofemoral angle, and congruence angle (p–values: 0.358, 0.995, 0.912, 0.802, 1.000, and 0.907, respectively). The mean Q angle was measured as 15.6 ± 2.2 degrees in the OSD group and 14.3 ± 2.5 degrees in the control group (p = 0.014). Despite the difference being statistically significant, it was clinically insignificant because the difference was only 1.3 degrees. The principle etiologic factor seems to be increased physical activity rather than subtle variations is patellofemoral anatomy and alignment of extensor mechanism.

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Peri-implantitis and extracellular matrix antibodies: A case–control study

European Journal of Dentistry ◽

10.4103/ejd.ejd_28_17 ◽

2017 ◽

Vol 11 (03) ◽

pp. 340-344 ◽

Cited By ~ 5

Author(s):

Piero Papi ◽

Stefano Di Carlo ◽

Daniele Rosella ◽

Francesca De Angelis ◽

Mario Capogreco ◽

...

Keyword(s):

Extracellular Matrix ◽

Case Control Study ◽

Test Group ◽

Case Control ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Fisher Exact Test ◽

Type I ◽

Significant Difference ◽

Control Study

ABSTRACT Objective: The aim of this case–control study was to compare patients with a healthy peri-implant environment and patients affected by peri-implantitis, evaluating the occurrence of antibodies to extracellular matrix (ECM) molecules. The authors hypothesized the presence of ECM autoantibodies in serum of peri-implantitis patients. Materials and Methods: Patients were divided into two groups: one with dental implants with a diagnosis of peri-implantitis and one control group with implants classified as being “healthy.” Enzyme-linked immunosorbent assay was performed on patients' sera to detect human antibodies to type I, III, IV, and V collagens, laminin, and fibronectin. Fisher exact test was performed to evaluate statistical association, with a significant P < 0.05. Results: Forty-two patients were enrolled in this study, 27 females (64.28%) and 15 males (35.72%) with a mean age of 53 ± 29.69 years (age range 32–74). The presence of antibodies to CIII was recorded in 6/21 (28.57%) patients of test group, compared to just 2/21 (9.52%) for the control group, showing a statistically significant difference (P < 0.05). Other antibodies tested were found to be not statistically significant or absent. Conclusions: Within the limitations of this study, it can be concluded that further studies, with larger sample and different design, are necessary to address the research purpose, evaluating possible associations between anti-ECM antibodies and peri-implantitis.

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Hair nicotine concentration of cats with gastrointestinal lymphoma and unaffected control cases

Veterinary Record ◽

10.1136/vr.105564 ◽

2020 ◽

Vol 186 (13) ◽

pp. 414-414 ◽

Cited By ~ 1

Author(s):

Victoria Smith ◽

Clare Knottenbelt ◽

David Watson ◽

Dominic J Mellor ◽

Alexandra Guillen Martinez ◽

...

Keyword(s):

Case Control Study ◽

Control Group ◽

Gastrointestinal Lymphoma ◽

Control Groups ◽

Nicotine Concentration ◽

Hair Samples ◽

Significant Difference ◽

Unaffected Control ◽

And Control ◽

Control Study

BackgroundA previous study showed an association between owner-reported exposure to environmental tobacco smoke (ETS) and lymphoma in cats. This study aimed to investigate the association between ETS exposure and gastrointestinal lymphoma in cats, using hair nicotine concentration (HNC) as a biomarker.MethodsThis was a prospective, multi-centre, case–control study. Gastrointestinal lymphoma was diagnosed on cytology or histopathology. Hair samples were obtained from 35 cats with gastrointestinal lymphoma and 32 controls. Nicotine was extracted from hair by sonification in methanol followed by hydrophilic interaction chromatography with mass spectrometry. Non-parametric tests were used.ResultsThe median HNC of the gastrointestinal lymphoma and control groups was not significantly different (0.030 ng/mg and 0.029 ng/mg, respectively, p=0.46). When the HNC of all 67 cats was rank ordered and divided into quartiles, there was no significant difference in the proportion of lymphoma cases or controls within these groups (p=0.63). The percentage of cats with an HNC≥0.1 ng/mg was higher for the lymphoma group (22.9%) than the control group (15.6%) but failed to reach significance (p=0.45).ConclusionA significant association was not identified between HNC (a biomarker for ETS) and gastrointestinal lymphoma in cats; however, an association may exist and further studies are therefore required.

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Assesment of Endometrial Vascularization with Immunohistochemical CD34 Assay in Recurrent Pregnancy Loss: A Case Control Study

Gynecology Obstetrics and Reproductive Medicine ◽

10.21613/gorm.2016.664 ◽

2017 ◽

pp. 1

Author(s):

Emine Aydın ◽

Taner Usta

Keyword(s):

Pregnancy Loss ◽

Recurrent Pregnancy Loss ◽

Case Control Study ◽

Transmembrane Protein ◽

Vessel Diameter ◽

Control Group ◽

Control Groups ◽

Significant Difference ◽

And Control ◽

Control Study

Objectives: We compared the endometrial vascularization in hysteroscopic endometrial samplings between recurrent pregnancy loss (RPL) and control group.Study Design: We prospectively evaluated hysteroscopic endometrial samplings from RPL and control groups. CD34 transmembrane protein was used for evaluating endometrial vascularization. The vascularization was assessed based on thickness of vessels, diameter of the largest vessel, and number of vessels per mm2 in CD34-stained slides.Results: There was no significant difference in demographic findings and vascularization, such as largest vessel diameter (p: 0.572), and number of vessels per mm2 (p: 0.982) between the two groups.Conclusion: The cycling endometrium is a highly angiogenic tissue and may play a role in the etiology of RPL. However, we find a weak relationship between endometrial vascularization and RPL.

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