scholarly journals Seed viability of Dimorphandra gardneriana subject to water stress in different temperatures

2016 ◽  
Vol 46 (12) ◽  
pp. 2090-2095
Author(s):  
Marina Matias Ursulino ◽  
Maria do Perpétuo Socorro Damaceno Costa ◽  
José George Ferreira Medeiros ◽  
Edna Ursulino Alves ◽  
Paulo Costa Araujo ◽  
...  
Keyword(s):  
Polyethylene Glycol ◽  
Seed Viability ◽  
Forest Species ◽  
Water Restriction ◽  
Drastic Reduction ◽  
Germination Speed ◽  
Different Temperatures ◽  
Basic Studies ◽  
Polyethylene Glycol 6000 ◽  
Osmotic Potentials

ABSTRACT: The forest species Dimorphandra gardneriana Tul. is widely used for various pharmacological products, yet few basic studies have been undertaken to understand their ecological and physiological attributes under stress conditions. The goal of this research was to evaluate the seed germination and vigor when subjected to different osmotic potentials and temperatures. Water restriction was simulated with polyethylene glycol 6000 solution (PEG 6000) with osmotic potentials of 0; -0,2; -0,4; -0,6; -0,8, and -1,0MPa at temperatures of 20, 25, 30, and 35°C. The effect of the treatment was determined by the germination and vigor (germination speed index, length and phytomass of dry shoot and roots) of the seeds, in an entirely random design with four repetitions. From-0, 4MPa it occurs a drastic reduction in germination and vigor of seeds although these factors were less affected at temperature of 25°C up to -0, 2MPa.

scholarly journals Germination of Eugenia brasiliensis, E. involucrata, E. pyriformis, and E. uniflora (Myrtaceae) under water-deficit conditions

2019 ◽  
Vol 41 (1) ◽  
pp. 76-85 ◽  
Author(s):  
Mariane Cristina Inocente ◽  
Claudio José Barbedo
Keyword(s):  
Polyethylene Glycol ◽  
Water Deficit ◽  
Seedling Development ◽  
Water Restriction ◽  
Water Potentials ◽  
Normal Seedling ◽  
Polyethylene Glycol 6000 ◽  
Osmotic Potentials

Abstract: This work aims at evaluating the tolerance of seeds of Eugenia brasiliensis Lam., E. involucrata DC., E. pyriformis Camb., and E. uniflora L. (Myrtaceae) to water deficit. Germination was carried out in polyethylene glycol 6000 solutions, at different osmotic potentials (0.0, -0.5, -1.0, -1.5, -2.0, -3.0, -4.0, and -5.0 MPa). The seeds were also placed in trays containing sand as substrate, and the water was replenished at different times, for up to 34 days. Seeds were evaluated as for their ability to undergoing a deficit period, and of germinating when water was made available again. In general, Eugenia spp. seeds were able to germinate at up to -1.5 MPa. In water potentials lower than 0.0 MPa, the beginning of germination experienced a delay, and it became better distributed throughout time. In the trays, the water restriction for up to 16 days did not limit normal seedling development. Seeds of Eugenia spp. were considerably tolerant to water deficit, as for both intensity and duration, which guarantees the development of seedlings and the propagation of the species.

scholarly journals Seeds of Caesalpinia echinata Lam. Under Water Stress at Different Temperatures

2020 ◽  
Vol 12 (7) ◽  
pp. 66
Author(s):  
Caroline Marques Rodrigues ◽  
Edna Ursulino Alves ◽  
Rosemere dos Santos Silva ◽  
Flávio Ricardo da Silva Cruz ◽  
Maria Lúcia Maurício da Silva ◽  
...  
Keyword(s):  
Water Stress ◽  
Economic Value ◽  
Seed Vigor ◽  
Large Tree ◽  
Randomized Design ◽  
Different Temperatures ◽  
C 30 ◽  
Caesalpinia Echinata ◽  
Polyethylene Glycol 6000 ◽  
Osmotic Potentials

The Caesalpinia echinata Lam. species, belongs to the Fabaceae family, popularly known as Pau Brazil, is a large tree, native to Brazil, that can be found from Ceará to Rio de Janeiro, with a relevant national historic and economic value. The objective of this study was to evaluate the effects of water stress at different temperatures on germination and seed vigor. The experiment was carried out at the Laboratório de Análise de Sementes of the Centro de Ciências Agrárias of the Universidade Federal da Paraíba, Areia-Paraíba. The water stress was formulated with solutions of polyethylene glycol 6000 (PEG 6000) in different osmotic potentials of 0.0 (control), -0.2; -0.4; -0.6 MPa at constant temperatures of 25, 30 and 35 °C, in a completely randomized design. The percentage of germination, first counting of germination, germination speed index, length and dry matter of seedlings were evaluated to determine the effects of the treatments. Decreases of the osmotic potential negatively influenced the germination and vigor of C. echinata seeds at the three evaluated temperatures (25 ºC, 30 ºC, 35 °C).

Effect of osmotic stress on Myrtus communis germination

Biologia ◽  
2012 ◽  
Vol 67 (1) ◽  
Author(s):  
Hamrouni Lamia ◽  
Souayah Naoufel ◽  
Khouja Larbi ◽  
Rejeb Néjib
Keyword(s):  
Polyethylene Glycol ◽  
Water Potential ◽  
Germination Percentage ◽  
Myrtus Communis ◽  
Intraspecific Variations ◽  
Germination Ability ◽  
Germination Speed ◽  
Petri Dishes ◽  
Genetic Pool ◽  
Polyethylene Glycol 6000

AbstractThe effects of water potential on germination were studied in four provenances of Myrtus from different bioclimatic zones in Tunisia. Water potential between 0 and −10 bars were obtained using polyethylene glycol 6000 (PEG-6000) solutions. Seeds were planted in Petri dishes full of sterile perlite, and kept for one month under 1500 lux light with 16h photoperiod and at 25°C. A low water potential induced a reduction in germination percentage and germination speed. Significant variations between the provenances were found even under control conditions, especially at the speed germination level. The provenances from humid regions were the most susceptible to water stress, and their germination percentage showed significant reductions at −8 bars. These differences in germination ability of provenances might be attributed to intraspecific variations resulting from the effects of natural selection and genetic pool background.

scholarly journals Priming corn seeds with plant growth regulator

2016 ◽  
Vol 38 (3) ◽  
pp. 227-232 ◽  
Author(s):  
Dryelle Sifuentes Pallaoro ◽  
Anne Caroline Dallabrida Avelino ◽  
Elisangela Clarete Camili ◽  
Sebastião Carneiro Guimarães ◽  
Maria Cristina de Figueiredo e Albuquerque
Keyword(s):  
Polyethylene Glycol ◽  
Plant Growth ◽  
Gibberellic Acid ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Dry Weight ◽  
Control Group ◽  
Water Restriction ◽  
Polyethylene Glycol 6000 ◽  
Different Doses

Abstract This study aimed to evaluate the plant growth regulator application, in different doses, on priming, with and without water restriction, in corn seeds. Evaluations were carried out in two periods (0 to 30 days of storage), with treatments consisting of seeds primed in water (0.0 MPa) and polyethylene glycol 6000 solution (-0.4 MPa), with or without plant growth regulator added in different doses, plus a control group. The amount of plant growth regulator was standardized by the gibberellic acid concentration in the commercial product, adopting the doses zero, 1, 2 and 3 mg of gibberellic acid. L-1 solution. After priming, the seeds were dried in an oven with forced air circulation for nine hours, and evaluated for water content, percentage and first count of germination, percentage of abnormal seedlings and non-germinated seeds, cold test, seedling length and dry weight and vigor index. Priming with water restriction -0.4 MPa, in solution of polyethylene glycol 6000, improves the vigor; however, the addition of plant growth regulator in priming does not provide increase in corn seeds quality. Corn priming in water, regardless the addition of plant growth regulator, reduces the ability of the seeds to withstand storage.

scholarly journals Physiological quality of seed and seedling performance of crambe genotypes under water stress

2017 ◽  
Vol 21 (3) ◽  
pp. 175-179 ◽  
Author(s):  
Fernando H. B. Machado ◽  
Andréia M. S. de S. David ◽  
Lucas V. S. Cangussú ◽  
Josiane C. Figueiredo ◽  
Hugo T. R. Amaro
Keyword(s):  
Polyethylene Glycol ◽  
Water Stress ◽  
Randomized Design ◽  
Physiological Quality ◽  
Completely Randomized Design ◽  
And Performance ◽  
Osmotic Agents ◽  
Polyethylene Glycol 6000 ◽  
Osmotic Potentials

ABSTRACT Water stress is a condition that causes physiological changes in different species and even genotypes of the same species. One of the osmotic agents most used to simulate this condition is polyethylene glycol 6000. The aim of this study was to evaluate the effects of water stress on the physiological quality of seeds and performance of seedlings of crambe genotypes. A completely randomized design was used, in a factorial scheme with two genotypes (one cultivar and one line) and five osmotic potentials simulated with aqueous solutions of polyethylene glycol 6000 (control = 0, -0.25, -0.50, -1.0, -1.50 MPa), with four replicates of 50 seeds per treatment. The effects of the treatments were evaluated by means of germination, first count, germination speed index and fresh and dry matter of seedlings. The physiological quality of seeds and the performance of crambe seedlings are negatively affected under water stress from -0.25 MPa on. Seeds of the genotype FMSCR 1101 have greater tolerance to drought stress, regardless of the osmotic potential used.

EFFECTS OF TEMPERATURE AND OSMOTIC POTENTIAL ON GERMINATION OF SUNFLOWER AND SAFFLOWER AND ON HORMONE-TREATED SUNFLOWER SEEDS

1978 ◽  
Vol 58 (2) ◽  
pp. 295-301 ◽  
Author(s):  
M. MAFTOUN ◽  
A. R. SEPASKHAH
Keyword(s):  
Acetic Acid ◽  
Polyethylene Glycol ◽  
Carthamus Tinctorius ◽  
Naphthalene Acetic Acid ◽  
Sunflower Seeds ◽  
Salt Tolerant ◽  
Indole Butyric Acid ◽  
Effects Of Temperature ◽  
Polyethylene Glycol 6000 ◽  
Osmotic Potentials

The influence of three temperatures and solutions of different osmotic potentials obtained with NaCl and/or polyethylene glycol 6000 was tested on the germination of sunflower (Helianthus annuus L.) and safflower (Carthamus tinctorius L.) seeds. The effects of two hormones at two concentrations on the germination of osmotically stressed sunflower seeds were also investigated. Seeds of the Record cultivar of sunflower were more salt-tolerant than Chernianka-66. Furthermore, Record germinated better at 20 °C than at 10° or 30 °C, while Chernianka-66 showed higher germination at 20° and 30 °C and was most sensitive to salinity at 10 °C. Seeds from Ute safflower exhibited more salt tolerance than did Local 3151, especially at the lower NaCl-induced osmotic potentials. The two safflower cultivars germinated better at 10° and 20 °C than at 30 °C. Seed germination of both crops was restricted more with polyethylene glycol 6000 than with NaCl, presumably due to the greater water stress caused by the former osmotic substrate. Germination of Record in NaCl substrate was inhibited at 50 ppm α-naphthalene acetic acid (NAA). NAA at 50 ppm decreased the germination of cv. Chernianka-66, whereas 3-indole butyric acid (IBA) at 10 ppm enhanced its germination. In general, germination of both sunflower cultivars was stimulated more by IBA than NAA. Furthermore, seeds treated with 10 ppm concentration gave better response than those treated with 50 ppm, possibly due to toxic effects.

scholarly journals SUBSTRATES AND TEMPERATURES FOR THE GERMINATION OF SEEDS OF Senegalia tenuifolia (L.) BRITTON & ROSE

2016 ◽  
Vol 29 (1) ◽  
pp. 113-118 ◽  
Author(s):  
ALCIMONE MARIA SILVA ARAÚJO2 ◽  
LIZ CAROLINA DA SILVA LAGOS CORTES ASSIS2 ◽  
NARJARA WALESSA NOGUEIRA ◽  
RÔMULO MAGNO OLIVEIRA DE FREITAS ◽  
SALVADOR BARROS TORRES
Keyword(s):  
Significant Interaction ◽  
Dry Mass ◽  
Shoot Length ◽  
Forest Species ◽  
Germination Test ◽  
Tukey Test ◽  
Seed Analysis ◽  
Germination Speed ◽  
Two Factors ◽  
Different Temperatures

ABSTRACT: The Rules for Seed Analysis and the Instructions for Seed Analysis of Forest Species have no recommendations for conducting the S. tenuifolia germination test. Therefore, the aim of the present study was to evaluate different temperatures and substrates to perform the germination test of S. tenuifolia seeds. The experimental design was completely randomized in a factorial 4 × 3 design and consisted of four substrates (paper roll; on paper; on sand and on vermiculite) and three temperatures (25, 30 and 35 °C), with four replicates of 25 seeds. The percentage of normal seedlings, the germination speed index, the shoot length, root length and dry mass of seedlings were evaluated. The Tukey test was used at 5% probability. There was a significant interaction between the temperatures and substrates tested for all variables, indicating that there is at least one ideal combination of the two factors that can increase the germination of seeds. The germination and vigor of S. tenuifolia seeds are influenced by the temperature and by the substrate used in the germination test. The combination of the paper roll substrates with a temperature of 25 and 30 °C was suitable for the germination of S. tenuifolia seeds.

scholarly journals Seed physiological quality and seedling growth of pea under water and salt stress

2020 ◽  
Vol 24 (2) ◽  
pp. 95-100 ◽  
Author(s):  
Isabella C. Pereira ◽  
Hugo C. R. M. Catão ◽  
Franciele Caixeta
Keyword(s):  
Polyethylene Glycol ◽  
Sodium Chloride ◽  
Potassium Chloride ◽  
Seedling Growth ◽  
Dry Matter ◽  
Chloride Solutions ◽  
Randomized Experimental Design ◽  
Physiological Quality ◽  
Polyethylene Glycol 6000 ◽  
Osmotic Potentials

ABSTRACT The aim of this study was to evaluate seed physiological quality and initial pea (Pisum sativum) seedling development at different water and salt potentials. Polyethylene glycol 6000, sodium chloride and potassium chloride solutions with different osmotic potentials (0; -0.2; -0.4; -0.6; -0.8; -1.0, and -1.2 MPa) were used, and the experiment had a 3 × 7 factorial arrangement. The physiological quality and initial seedling growth characteristics (first germination count, final germination count, strong normal seedlings, epicotyl and taproot lengths, epicotyl dry matter, and root dry matter) were evaluated in a completely randomized experimental design, with four repetitions. Decreases in the observed variables showed that the simulated stress conditions negatively affected physiological performance, germination and initial seedling growth. The water and salt stresses induced by the sodium chloride and potassium chloride solutions were greater than the stress induced by polyethylene glycol 6000.

Hepatitis a Virus Concentration from Experimentally Contaminated Distilled, Tap, Waste and Seawater

1989 ◽  
Vol 21 (3) ◽  
pp. 255-258 ◽  
Author(s):  
Evangélos Biziagos ◽  
Jacques Passagot ◽  
Jean-Marc Crance ◽  
Robert Deloince
Keyword(s):  
Cell Culture ◽  
Polyethylene Glycol ◽  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Tap Water ◽  
Virus Concentration ◽  
Beef Extract ◽  
Polyethylene Glycol 6000

The concentration of cell-culture-adapted hepatitis A virus (HAV) from experimentally contaminated distilled, drinking, waste and seawater was performed by using a filter adsorption-elu-tion method in the following conditions: HAV seeded in water was adsorbed at pH 4.0 to two nitrocellulose membranes (1.2 and 0.45 µm porosity for distilled and tap water or 8.0 and 3.0 µm porosity for waste and seawater), then eluted by 3% beef-extract at pH 8.5 and further concentrated by polyethylene glycol 6000 precipitation. Thus, HAV in 5 to 50 liters of seeded waters was concentrated approximately 1,700 to 17,000 fold with greater than 70% recovery of the initial virus added to the samples.

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