scholarly journals Seeds of Caesalpinia echinata Lam. Under Water Stress at Different Temperatures

2020 ◽  
Vol 12 (7) ◽  
pp. 66
Author(s):  
Caroline Marques Rodrigues ◽  
Edna Ursulino Alves ◽  
Rosemere dos Santos Silva ◽  
Flávio Ricardo da Silva Cruz ◽  
Maria Lúcia Maurício da Silva ◽  
...  

The Caesalpinia echinata Lam. species, belongs to the Fabaceae family, popularly known as Pau Brazil, is a large tree, native to Brazil, that can be found from Ceará to Rio de Janeiro, with a relevant national historic and economic value. The objective of this study was to evaluate the effects of water stress at different temperatures on germination and seed vigor. The experiment was carried out at the Laboratório de Análise de Sementes of the Centro de Ciências Agrárias of the Universidade Federal da Paraíba, Areia-Paraíba. The water stress was formulated with solutions of polyethylene glycol 6000 (PEG 6000) in different osmotic potentials of 0.0 (control), -0.2; -0.4; -0.6 MPa at constant temperatures of 25, 30 and 35 °C, in a completely randomized design. The percentage of germination, first counting of germination, germination speed index, length and dry matter of seedlings were evaluated to determine the effects of the treatments. Decreases of the osmotic potential negatively influenced the germination and vigor of C. echinata seeds at the three evaluated temperatures (25 ºC, 30 ºC, 35 °C).

2019 ◽  
Vol 40 (6Supl2) ◽  
pp. 2877
Author(s):  
Maria das Graças Rodrigues Nascimento ◽  
Maria Lúcia Maurício da Silva ◽  
Edna Ursulino Alves ◽  
Caroline Marques Rodrigues

Germination is negatively influenced by water scarcity and by temperatures above or below the optimal range of the species, so it is necessary to know the critical point of absorption for the species or cultivar. The objective of this work was to evaluate the effect of water stress, simulated by solutions of polyethylene glycol 6000 (PEG 6000) at different temperatures, on the germination and vigor of seeds of four cultivars (Branca, Orelha de Vó, Rosinha and Roxinha) of beans (Phaseolus lunatus L.). The water stress simulation was performed using PEG 6000 solutions at concentrations of 0.0, -0.2, -0.4, -0.6, -0.8, -1.0, and -1.2 MPa at temperatures of 25, 30, and 35 ° C. The analyzed variables were percentage of germination at first count, germination speed index, length of aerial part and primary root of seedlings, and dry mass of the respective parts of the seedlings. The seeds of the cultivar Orelha de Vó were more sensitive to water stress, with 88% germinating up to the potential of -0.6 MPa at the tested temperatures. The cultivars Branca, Rosinha, and Roxinha germinated (94, 100, and 100%, respectively) up to the potential of -0.8 MPa. Water stress simulated by PEG (6000) negatively affects seed twinning and seed vigor of bean cultivars.


Author(s):  
Paulo C. S. Santos ◽  
Clarisse P. Benedito ◽  
Tatianne R. C. Alves ◽  
Emanoela P. Paiva ◽  
Erivanessa C. Sousa ◽  
...  

ABSTRACT Water availability and temperature are among the main abiotic factors that influence seed germination and vigor, since they act directly on biochemical and physiological processes, which result in the production of the primary root. The objective of this study was to verify the effects of stress on germination and vigor of H. impetiginosus (‘ipê-roxo’) seeds under different temperatures and osmotic agents. The experimental design was completely randomized, in a 6 x 2 factorial scheme (osmotic potentials x temperatures) with four replicates of 25 seeds for each osmotic agent. In order to simulate water stress, polyethylene glycol (PEG 6000) and mannitol solutions were used and distilled water was used as control. Osmotic potentials of 0, -0.2, -0.4, -0.6, -0.8 and -1.0 MPa were evaluated at temperatures of 25 and 30 °C, under 8 h photoperiod. The variables analyzed were: germination, germination speed index, shoot length, root length, and total dry matter of seedlings. Simulated water stress affected seed germination and seed vigor of H. impetiginosus at both temperatures and osmotic agents from -0.6 MPa.


Author(s):  
José E. S. B. da Silva ◽  
Emanoela P. de Paiva ◽  
Moadir de S. Leite ◽  
Salvador B. Torres ◽  
Maria L. de Souza Neta ◽  
...  

ABSTRACT This study aimed to evaluate the effect of physiological priming with salicylic acid on the mitigation of water and salt stresses on onion (Allium cepa L.) seeds. For this, seeds of onion, cultivar IPA 11, were soaked in salicylic acid solution (50 µM) for 24 h and placed to germinate at different water and saline potentials. For water stress, the experiment was conducted in a completely randomized design, in a 2 x 5 factorial scheme (salicylic acid with 0 and 50 µM and osmotic potentials of 0, -0.1, -0.2, -0.3, -0.4 MPa induced by polyethylene glycol 6000), with four repetitions of 50 seeds per plot. In salt stress, the adopted experimental design was completely randomized, with treatments distributed in a 2 x 4 factorial scheme (salicylic acid at concentrations of 0 and 50 µM and the osmotic potentials of 0, -0.3, -0.6 and -0.9 MPa induced by NaCl), in four repetitions of 50 seeds. For both experiments, germination, growth and accumulation of organic solutes were evaluated. Salicylic acid did not stimulate the germination of onion seeds in the absence of stress, but the application of 50 µM of salicylic acid increased the germination speed, growth and dry matter accumulation under conditions of water and salt stress. Onion plants were efficient in performing osmotic adjustment up to the potentials of -0.2 MPa induced by PEG 6000 under water stress and of -0.4 MPa induced by NaCl under salt stress.


Author(s):  
Fernando H. B. Machado ◽  
Andréia M. S. de S. David ◽  
Lucas V. S. Cangussú ◽  
Josiane C. Figueiredo ◽  
Hugo T. R. Amaro

ABSTRACT Water stress is a condition that causes physiological changes in different species and even genotypes of the same species. One of the osmotic agents most used to simulate this condition is polyethylene glycol 6000. The aim of this study was to evaluate the effects of water stress on the physiological quality of seeds and performance of seedlings of crambe genotypes. A completely randomized design was used, in a factorial scheme with two genotypes (one cultivar and one line) and five osmotic potentials simulated with aqueous solutions of polyethylene glycol 6000 (control = 0, -0.25, -0.50, -1.0, -1.50 MPa), with four replicates of 50 seeds per treatment. The effects of the treatments were evaluated by means of germination, first count, germination speed index and fresh and dry matter of seedlings. The physiological quality of seeds and the performance of crambe seedlings are negatively affected under water stress from -0.25 MPa on. Seeds of the genotype FMSCR 1101 have greater tolerance to drought stress, regardless of the osmotic potential used.


2018 ◽  
Vol 40 (1) ◽  
pp. 36-43 ◽  
Author(s):  
Ayslan Trindade Lima ◽  
Paulo Henrique de Jesus da Cunha ◽  
Bárbara França Dantas ◽  
Marcos Vinicius Meiado

Abstract: Seed hydration memory is the ability of seeds to retain biochemical and physiological changes caused by discontinuous hydration. This study aimed to determine if Senna spectabilis (DC.) H.S. Irwin & Barneby var. excelsa (Schrad.) H.S.Irwin & Barneby (Fabaceae) present seed memory and evaluate the effects of hydration and dehydration cycles (HD) on the seed germination of this species when submitted to conditions of water stress. Seeds underwent HD cycles (0, 1, 2 and 3 cycles) corresponding to the hydration times X (6 hours), Y (16 hours) and Z (24 hours), determined from the imbibition curve, with 5 hours of dehydration and submitted to water stress conditions. Germination was evaluated at 0.0, -0.1, -0.3, -0.6 and -0.9 MPa, obtained with polyethylene glycol 6000 solution. Germinability (%), mean germination time (days) and hydrotime (MPa d-1) were calculated. The seeds of S. spectabilis var. excelsa are sensitive to the low osmotic potentials tested in this study, however, when submitted to the HD cycles of 16 hours hydration (time Y), the tolerance to water stress conditions is increased. In addition, the observed benefits on the evaluated germination parameters show that S. spectabilis var. excelsa present seed hydation memory.


FLORESTA ◽  
2019 ◽  
Vol 50 (1) ◽  
pp. 923
Author(s):  
Ademir Kleber Morbeck Oliveira ◽  
Jose Carlos Pina

Germination is considered a critical step in the seedling production process aiming the recovery of environments. Thus, understanding the germination behavior of native species with potential for economic use contributes to the development of the forest seed and seedling chain. Considering the limited information on the tree species Pouteria torta subesp. torta, this study aimed to evaluate its seed germination and formation of normal seedlings using different temperatures and substrates. The experiment was conducted in germination chamber in a research laboratory using two substrates (paper and vermiculite) and six temperatures (20, 25, 30, 35, 20-30 and 25-35 °C) in a completely randomized design in a 2x6 factorial arrangement. Daily evaluation was conducted to verify primary root emission, seed vigor, and seedling formation. The results indicated that the primary root protrusion process began after 20 days of testing under the best conditions, with germination time lasting longer than two months. The results obtained also showed that there was interaction between factors, with use of the vermiculite substrate and the temperatures of 35 and 25-35 °C producing the best germination rates and germination speed index, and use of the paper substrate and the temperatures of 30 and 35 °C showing the best mean germination time. Considering the analyzed variables (germination, germination speed index, and formation of normal seedlings), the temperatures of 35 and 25-35 °C and the vermiculite substrate are the most suitable for the production of Pouteria torta subesp. torta seedlings.


Author(s):  
Fabrícia N. de Oliveira ◽  
Jessica R. de Oliveira ◽  
Salvador B. Torres ◽  
Rômulo M. O. de Freitas ◽  
Narjara W. Nogueira

ABSTRACT The germination of seeds is affected by many factors, including water availability and temperature. This study aimed to evaluate the effects of water stress and different temperatures on germination and initial development of S. gardneriana M. R. Barbosa & Peixoto seedlings. The experiment was carried out in a completely randomized design, in a 7 x 4 factorial scheme, with seven potentials (0; -0.1; -0.2; -0.3; -0.4; -0.5 and - 0.6 MPa) and four temperatures (25, 30, 35 and 20-30 °C), with four replicates of 25 seeds. The analyzed variables were germination, percentage of normal seedlings, germination speed index, seedling length (shoot and root) and dry weight (shoot and root). There is a decrease in the germination and vigor of the seeds as the water potential decreases, being less affected under the temperature of 25 °C and very accelerated at 35 °C. The tolerance of S. gardneriana seed germination to water stress is variable between the temperatures and limiting to the formation of normal seedlings from the potential -0.5 MPa at temperatures of 25 to 30 °C and -0.4 and -0.2 MPa for 20-30 and 35 °C, respectively.


2011 ◽  
Vol 68 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Carlos Henrique Salvino Gadelha Meneses ◽  
Riselane de Lucena Alcântara Bruno ◽  
Pedro Dantas Fernandes ◽  
Walter Esfrain Pereira ◽  
Leonardo Henrique Guedes de Morais Lima ◽  
...  

The physiological quality of cotton cultivar seeds (Gossypium hirsutum var. latifolium L.) was evaluated in laboratory by the simulation of water potentials with polyethyleneglycol-6000 (0.0; -0.2; -0.4; -0.6; -0.8 and -1.0 MPa), at 25ºC using germitest paper as substrate. A completely randomized design in a 4 × 6 factorial scheme with four replications of 50 seeds each was used. The studied variables were: germination percentage, first count of germination, germination velocity index, accelerated aging in water, electrical conductivity, humidity, vigor classification, radicle length and radicle/shoot length ratio. The effect of water stress on seed viability and on plantlet vigor was severe at potentials below -0.4 MPa. The 'CNPA 187 8H' cultivar was the least sensitive to the tested osmotic potentials, both in terms of germination and of vigor. The 'BRS-201' cultivar was mostly affected by the viability and vigor tests under water deficit conditions. Differential viability and vigor between cultivars were observed under the water stress levels.


2016 ◽  
Vol 18 (3) ◽  
pp. 133
Author(s):  
Ali Djunaedi

Silase ikan sebagai pakan buatan Artemia sp mempunyai beberapa keunggulan diantaranya memiliki kandungan nutrisi yang tinggi dan kontinuitas ketersedian sebagai pakan dapat terpenuhi. Keunggulan yang dimiliki silase ikan ini sangat tepat diterapkan dalam menunjang pertumbuhan Artemia sp. Penelitian ini bertujuan untuk megetahui pengaruh berbagai dosis silase ikan terhadap pertumbuhan Artemia sp. Metode yang digunakan adalah eksperimental laboratories. Perlakuan yang diterapkan terdiri dari 4 perlakuan yaitu pemberian silase ikan A (10 mg/L), B (20 mg/L), C (30 mg/L) dan D (40 mg/L) dengan ulangan masing-masing 3 kali. Parameter ukur adalah Pertambahan berat mutlak, pertambahan panjang dan tingkat kelulushidupan. Hasil pengamatan menunjukkan bahwa rata-rata pertambahan berat mutlak dan pertumbuhan panjang tertinggi diperoleh perlakuan D yaitu 7,99 ± 0,05 mg dan 8,8 ± 0,25 mm, terendah diperoleh perlakuan A yaitu 6,46 ± 0,27 mg dan 7,2 ± 0,32 mm serta untuk tingkat kelulushidupan tertinggi diperoleh perlakuan D yaitu 31,09 ± 1,29 %, terendah diperoleh perlakuan A yaitu 9,67 ± 2,96 %.Kata Kunci : Artemia sp. Silase, Laju Pertumbuhan, Tingkat KelulushidupanSilase is a liquid protein made from trash fish which is decomposed by enzims. Silase has some advantages ie, high nutrition value, easy to produce, economic value and could be produce easily. These could be aplied to support the growth of Artemia sp. The aim of this research is to investigate effect of the various dose of silase on the growth level of Artemia sp. These research was conducted at Laboratorium Pakan Alami Balai Besar Pengembangan Budidaya Air Payau(BBPAP), Jepara. Experimental laboratories was used with completely randomized design. The treatments were doses of silase : A (10 mg/L); B (20 mg/L); C (30 mg/L) and D (40 mg/L). The individual weight, length and survival rate were measured. The result showed that the highest average of individual absolute growth (weight) was reached on treatment D (7,99 + 0,05 mg), while the lowest on treatment A (6,46 + 0,27 mg). The highest length growth was reached on treatment D (8,8 + 0,25 mm), while the lowest on theatment A (7,2 + 0,32 mm). The highest survival rate of Artemia sp was reached on treatment D (31,09 + 1,29%) and the lowest on treatment A (9,67 + 2,96%).Keywords : Artemia sp, Silase, Growth rate, Survival rate


2016 ◽  
Vol 46 (12) ◽  
pp. 2090-2095
Author(s):  
Marina Matias Ursulino ◽  
Maria do Perpétuo Socorro Damaceno Costa ◽  
José George Ferreira Medeiros ◽  
Edna Ursulino Alves ◽  
Paulo Costa Araujo ◽  
...  

ABSTRACT: The forest species Dimorphandra gardneriana Tul. is widely used for various pharmacological products, yet few basic studies have been undertaken to understand their ecological and physiological attributes under stress conditions. The goal of this research was to evaluate the seed germination and vigor when subjected to different osmotic potentials and temperatures. Water restriction was simulated with polyethylene glycol 6000 solution (PEG 6000) with osmotic potentials of 0; -0,2; -0,4; -0,6; -0,8, and -1,0MPa at temperatures of 20, 25, 30, and 35°C. The effect of the treatment was determined by the germination and vigor (germination speed index, length and phytomass of dry shoot and roots) of the seeds, in an entirely random design with four repetitions. From-0, 4MPa it occurs a drastic reduction in germination and vigor of seeds although these factors were less affected at temperature of 25°C up to -0, 2MPa.


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