Application of multivariate techniques in the evaluation of pure lines of beans

ABSTRACT: The objective of this study was to apply multivariate techniques, canonical discriminant analysis, and multivariate contrasts, indicating the most favorable inferences in the evaluation of pure lines of beans. The study was conducted at the experimental field of the Institute for Breeding and Molecular Genetics, in Lages, SC, Brazil. The experiment was composed of 24 pure lines of beans from the Santa Catarina test of cultivars. Plant height, numbers of pods and grains per plant, and stem diameter were the variables measured. The complete randomized block design was used with four replications. The data were subjected to multivariate analysis of variance, canonical discriminant analysis, multivariate contrasts and univariate contrasts. The first canonical discriminant function has captured 81% of the total variation in the data. The Scott-Knott test showed two groups of inbred lines at the average -of scores of the first canonical discriminant function. It was considered that testing hypotheses with the canonical scores may result in loss of information obtained from the original data. Multivariate contrasts indicated differences within the group formed by the Scott-Knott test. The canonical discriminant analysis and multivariate contrasts are excellent techniques to be combined in the multivariate assessment, being used to explore and test hypotheses, respectively.

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Diallel analysis of maize resistance to Phaeosphaeria maydis

Scientia Agricola ◽

10.1590/s0103-90162004000100007 ◽

2004 ◽

Vol 61 (1) ◽

pp. 36-42 ◽

Cited By ~ 7

Author(s):

Herberte Pereira da Silva ◽

José Roberto Moro

Keyword(s):

Disease Severity ◽

Block Design ◽

Genetic Resistance ◽

Diallel Analysis ◽

Inbred Lines ◽

Randomized Block Design ◽

Whole Plant ◽

Sources Of Variation ◽

Specific Hybrid ◽

Additive Genetic Effects

In breeding programs directed towards genetic resistance against diseases, the estimation of genetic parameters that control resistance allows the introduction of resistance into suscetible germplasm to be clearly focused. The purposes of this study were to estimate heterosis effects, and the general (GCA) and specific (SCA) combining abilities by using two rating methods for resistance to Phaeosphaeria maydis in a diallel analysis of 36 F1 maize hybrids and their nine inbred lines. Trials were conducted in three environments. Disease severity was evaluated in the whole plant (PI) and in the leaf positioned just below the point of insertion of the main ear (AFA). The trials followed a randomized block design with plots represented by a 5 m long rows. Differences among combining ability estimates for different environments and in both evaluation methods showed significant effects (P < 0.01) for environment (E), GCA, and GCA × E. The SCA, and SCA × E effects were not significant for any of the disease severity variables. The GCA effects were more important than SCA for this set of inbred lines, suggesting that additive genetic effects are the most important sources of variation for this trait. Heterosis effects for resistance were estimated, and it was possible to identify specific hybrid combinations between lines which have high potential for genetic control of this pathogen. Results for both disease severity variables were practically identical, even though the PI method was more convenient to use.

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Selection of Coffea canephora parents from the botanical varieties Conilon and Robusta for the production of intervarietal hybrids

Ciência Rural ◽

10.1590/0103-8478cr20170444 ◽

2018 ◽

Vol 48 (4) ◽

Author(s):

Leilane Nicolino Lamarão de Oliveira ◽

Rodrigo Barros Rocha ◽

Fábio Medeiros Ferreira ◽

Victor Mouzinho Spinelli ◽

André Rostand Ramalho ◽

...

Keyword(s):

Block Design ◽

Original Data ◽

Coffea Canephora ◽

Reference Points ◽

Hybrid Vigor ◽

Botanical Variety ◽

Randomized Block Design ◽

Main Component ◽

Botanical Varieties ◽

Selection Of

ABSTRACT: The objective of this research was to quantify the genetic divergence of potential Coffea canephora parents, with the goal of developing progenies that associate the best traits of the Conilon and Robusta botanical varieties for hybrid vigor expression. Thus, 10 morphological and productive characteristics of 130 clones of Conilon and Robusta botanical varieties and their intervarietal hybrids were evaluated over 2 years. The experiment was conducted in a randomized block design with four replicates of four plants per plot. For selection of parents, the main component analysis was used to associate values with reference points obtained from the average of each botanical variety. The first two principal components allowed for the separation of the botanical varieties representing the variability contained in the original data with 76% for the first year and 69% for the second year. Although, the genotype × years interaction had significant effects, there were minor differences in the grouping from one year to the next, which is associated with the higher repeatability estimates observed in this study. It was observed that crosses with the 16-1-81I, 9-1-82L, and 13-1-61I parents of the botanical variety Robusta and the 167I, 890E, and 130I parents of the Conilon botanical variety presented greater potential for obtaining selection gains.

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GGE Biplot projection in discriminating the efficiency of popcorn lines to use nitrogen

Ciência e Agrotecnologia ◽

10.1590/1413-70542017411030816 ◽

2017 ◽

Vol 41 (1) ◽

pp. 22-31 ◽

Cited By ~ 8

Author(s):

Adriano dos Santos ◽

Antônio Teixeira do Amaral Júnior ◽

Railan do Nascimento Ferreira Kurosawa ◽

Ismael Fernando Schegoscheski Gerhardt ◽

Roberto Fritsche Neto

Keyword(s):

Nitrogen Availability ◽

Block Design ◽

Germplasm Collection ◽

Inbred Lines ◽

Gge Biplot ◽

State University ◽

Information Ratio ◽

The North ◽

Randomized Block Design ◽

Main Components

ABSTRACT Nitrogen is essential for sustaining life on the planet, and it is the most important nutrient for obtaining high agricultural production. However, their use leads to the release of nitrous oxide with a global warming potential 296 times higher than the CO2 molecule, making it a challenge to reduce their use in agriculture. The objective of this research was to identify efficient popcorn inbred lines and responsive nitrogen use and exhibit a good expansion volume. For this, 29 inbred lines from the Germplasm Collection of Darcy Ribeiro North Fluminense State University (UENF) were evaluated at two contrasting levels of nitrogen availability (low and ideal) at two representative locations in the north and northwest of the state of Rio de Janeiro, Brazil, arranged in a randomized block design with three replicates. These inbred lines were discriminated against efficient use of nitrogen by multivariate GGE Biplot. Selective accuracy was close to 1, showing that the genotypes were enough to provide contrasting success in selection procedures. The first two main components (PC) retained 93.82% of the total variation, and PC1 furnished an information ratio (IR) that was unaffected by noise. L77 was the most unstable line, while P7, P2, P6, P3, P5, P4, P9, P10, P8, P9, L70, L74, and L55 were efficient and responsive. The GGE biplot method is recommended for the reliable identification of popcorn lines that are efficient and responsive to the use of nitrogen.

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Partial diallel and genetic divergence analyses in maize inbred lines

Acta Scientiarum Agronomy ◽

10.4025/actasciagron.v43i1.53540 ◽

2020 ◽

Vol 43 ◽

pp. e53540

Author(s):

Maria Fernanda de Souza Dias Maioli ◽

Ronald José Barth Pinto ◽

Tereza Aparecida da Silva ◽

Diego Ary Rizzardi ◽

Robson Akira Matsuzaki ◽

...

Keyword(s):

Grain Yield ◽

Genetic Divergence ◽

Block Design ◽

Inbred Lines ◽

Group Method ◽

Arithmetic Average ◽

Pair Group ◽

Maize Inbred Lines ◽

Randomized Block Design ◽

Partial Diallel

In this study, we aimed to estimate general and specific combining abilities (GCA and SCA, respectively) and to verify genetic divergence (Rogers distance, Unweighted Pair-Group Method Using Arithmetic Average - UPGMA) using microsatellite markers in maize inbred lines. Using a partial diallel scheme, a total of 19 inbred lines were crossed as (9 x 10), which were derived from the single hybrids SG6015 and P30F53, respectively. The 90 hybrids were evaluated in an incomplete randomized block design with common checks and three replications during the 2017-2018 growing season. Flowering time, average plant height, ear height insertion, average ear diameter, ear length, number of lodged and broken plants, mass of 100 grains and grain yield were measured. According to the analysis of variance, GCA, and SCA were significant (p < 0.05) in all the measured traits; inbred line B as well as 1 and 8, derived from the single hybrids SG6015 and P30F53, respectively, were selected due to their higher GCA values in grain yield to be used in crosses as testers, while the single cross hybrid (B x 1) was selected due to their higher SCA value in grain yield to be used in future breeding programs. The molecular marker analysis divided the inbred lines into two groups, where the highest dissimilarity (0.74) was observed between lines A and 9; however, these did not result in a high SCA value, therefore the hybrids obtained by such crossings were not selected for grain yield.

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Heterosis, inbreeding depression and their relationship with genetic divergence in sesame ( Sesamum indicum L.)

Acta Agronomica Hungarica ◽

10.1556/aagr.58.2010.3.15 ◽

2010 ◽

Vol 58 (3) ◽

pp. 313-321 ◽

Cited By ~ 1

Author(s):

P. Banerjee ◽

P. Kole

Keyword(s):

Plant Height ◽

Inbreeding Depression ◽

Seed Yield ◽

Seed Weight ◽

Block Design ◽

Basic Material ◽

Branch Number ◽

Randomized Block Design ◽

Component Traits ◽

Pure Lines

Seven parents (CST2002, MT34, OS-Sel-2, TKG22, AAUDT9304-14-4, B67 and Rama), their 21 F 1 s and 21 F 2 s were grown in summer 2003 in a randomized block design with three replications. Heterosis and inbreeding depression were studied for seven important yield-contributing characters (plant height, branch number plant −1 , capsules plant −1 , seeds capsule −1 , 1000-seed weight, stick yield plant −1 and seed yield plant −1 ). Maximum heterosis for seed yield plant −1 over the mid- and better-parent was recorded in CST2002×TKG22 (43.30%) and MT34×B67 (27.22%), respectively. Mid-parent heterosis for seed yield plant −1 was due to cumulative heterosis for various important component traits, such as capsules plant −1 , seeds capsule −1 and 1000-seed weight. Inbreeding depression was highest for seed yield, followed by 1000-seed weight, capsules plant −1 , branch number and plant height, indicating the predominance of non-additive genetic effects. B67×Rama exhibited significant positive heterosis in F 1 , but non-significant inbreeding depression in F 2 for seed yield. This cross can be utilized as basic material for identifying better pure lines. The clustering pattern indicated that in general genetically diverse parents exhibited more heterosis, as evident in the majority of the crosses.

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Physiological quality of soybean seeds stored after industrial treatments with different chemicals

Research Society and Development ◽

10.33448/rsd-v10i2.12279 ◽

2021 ◽

Vol 10 (2) ◽

pp. e6310212279

Author(s):

Renata Cristiane Pereira ◽

Lucas Caiubi Pereira ◽

Alessandro Lucca Braccini ◽

Larissa Vinis Correia ◽

Murilo Fuentes Pelloso ◽

...

Keyword(s):

Block Design ◽

Accelerated Aging ◽

Storage Period ◽

Original Data ◽

Soybean Seeds ◽

Randomized Block Design ◽

Seedling Length ◽

Physiological Quality ◽

Main Components

This study aimed to relate the smallest set of variables that compose the quality of soybean seeds lot under study, as well as to evaluate the influence of four industrial seed treatments and their respective slurry volumes on the physiological potential. The experiment was carried out in a completely randomized block design with 4 replicates and 24 treatments. The chemical treatments were: control (T1), micronutrient + polymer + drying powder (T2), bioregulator + polymer + drying powder (T3) and micronutrient + bioregulator + polymer + drying powder (T4). The seeds were stored for periods of 0, 15, 30, 45, 60 and 90 days and were subsequently evaluated for their physiological potential. In each storage period, the variable germination, first count, accelerated aging, emergence speed index, final emergence in the sand substrate, aerial part length, root length and total seedling length were evaluated. The main components of the standard germination test and first count were the ones that most contributed to explaining the variability of the original data. Increasing the volume of the slurry in the industrial seed treatment reduces the physiological quality of soybean seeds during storage.

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Diversity Analysis in Biofortified Inbred Lines of Maize (Zea mays L.)

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2021/v33i1630531 ◽

2021 ◽

pp. 115-123

Author(s):

Priyanka Jaiswal ◽

. Banshidhar ◽

Dan Singh Jakhar ◽

Rajesh Singh

Keyword(s):

Seed Weight ◽

Agricultural Research ◽

Block Design ◽

Inbred Lines ◽

Mean Value ◽

Randomized Block Design ◽

Zn Concentration ◽

Cluster Distance ◽

100 Seed Weight ◽

Selection Of

Thirty biofortified inbred lines of maize were evaluated for 11 parameters to study the genetic diversity by using D2 statistics during kharif 2017in Randomized Block Design (RBD) with three replications at Agricultural Research farm, Institute of Agricultural Sciences, BHU Varanasi. In present investigation all genotypes were grouped into ten cluster. Among the different clusters of inbred lines, the cluster II with 8 inbreds emerged as the largest cluster. The intra cluster D2 value ranged from 10.82 to 44.89. The maximum intra cluster distance was observed for cluster X (D2 = 44.89). The maximum inter cluster distance was observed between cluster V and VI (D2 = 180.90) followed by cluster V and VII (D2 = 166.10), cluster IV and V (D2 = 155.60), cluster V and VIII (D2 = 135.02) and cluster I and VI (D2 = 127.66). The maximum contribution towards divergence was due to 100 seed weight (52.18%), thus, estimates of variation in seed weight could be used as a basis for selection of distantly related parents. Highest mean value for grain yield per plant (80.8) and Zn concentration (39.53) were observed in cluster IV, while the highest mean value for 100 seed weight was found in cluster V. Therefore, these clusters prove to be of prime importance for selection of parents in hybridization programme aimed at higher yield along with enhanced grain Zn concentration.

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FLOWERING SYNDROME – HYBRID PERFORMANCE RELATIONSHIP IN MAIZE 1- Field traits and growth rates

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v47i4.518 ◽

2016 ◽

Vol 47 (4) ◽

Author(s):

Al- Khazaali & et al.

Keyword(s):

Grain Yield ◽

Dry Matter ◽

Growth Traits ◽

Block Design ◽

Inbred Lines ◽

High Yield ◽

Late Flowering ◽

Randomized Block Design ◽

Four Seasons ◽

Selection For

In order to identify the role of selection for early and late silking on the inbred lines from Maize (Zea Mays L.) regarding the grain yield of the consequent hybrids. Filed experiment for four seasons (spring and full 2014 and 2015) was conducted in the experimental field of Field Crop Dept., Coll. of Agric., Univ. of Baghdad. Four inbred lines (Zm19, Zm32, Zm51 and Zm61) and two testers (Zm21 late and Zm60 early silking) were used. In the first two seasons, the seeds of the four inbred lines were planted, first 10 plants that flowered early and 10 plants that flowered late were selected and the seeds of the selected plants were multiplied. The seeds of the early and late selected inbred lines were planted in the third season and crossed with testers. Crosses from the early and late flowering were produced and their seeds were cultivated in the last season. Comparison in some field and growth traits were conducted among the crosses. The experiments laid out in completely randomized block design (RCBD) at three replicates. Selection for early and late flowering parents has induced significant differences in days to flowering for all crosses and ranged between 62 days for early parents and 66 days for late parents. Also, significant differences were shown in several traits, the most prominent were crop’s and seed’s growth rate (CGR and SGR ), that correlated with seed filling duration (SFD), which contributed to increase the deposition of the dry matter into the grain for a longer period, which gave a high grain yield (GY). CGR of outstanding crosses ranged from 3.18 to 6.21 g.m-2.day, SGR ranged between 2.3 to 7.3 g. m-2.day, and the SFD between 35-38 days. In parallel, the testers (Zm21 late and Zm60 early silking) have significantly differed in their effect on the traits of some derived crosses, where the results showed significant superiority of the crosses Zm32Zm60 resulted from crossing the tester Zm60 with early silking parents selected from inbred line Zm32 on the crosses Zm32Zm21 resulted from crossing the tester Zm21 with the same parents of Zm32 in the traits of Total Dry Matter (TDM) (1.85 kg.m-2), CGR (18.28 g.m-2.day), Harvest Index (HI) (0.56), and SGR (3.3 g.m-2.day). It can be conclude that selection for early and late silking inbred lines, as well as the effect of the early and late silking tester have a great importance in deriving hybrids of a high yield and distinct in several traits, which are all significantly contributing in increasing the GY.

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Screening of maize germplasm for Turcicum leaf blight resistance

Journal of Applied and Natural Science ◽

10.31018/jans.v10i1.1586 ◽

2018 ◽

Vol 10 (1) ◽

pp. 98-101

Author(s):

T. A. Wani ◽

G. N. Bhat ◽

Mushtaq Ahmad ◽

A. Anwar ◽

Gul Zaffar

Keyword(s):

Block Design ◽

Inbred Lines ◽

Leaf Blight ◽

Blight Resistance ◽

Exserohilum Turcicum ◽

Maize Germplasm ◽

Randomized Block Design ◽

Maize Genotypes ◽

Sorghum Grains ◽

Moderately Resistant

: A study was conducted during Kharif 2012 and 2013 at Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar Campus Srinagar with the objective of screening sixty maize genotypes against Turcicum leaf blight caused by Exserohilum turcicum (Pass.) Leonard and Suggs. Field experiment was laid out in a randomized block design with three replications. In order to ensure establishment of infection, artificial inoculation of E. turcicum on test genotypes was made twice at 30 and 40 days after sowing with two different methods of inoculation (spraying on foliage of maize with spore suspension of pathogen @ 5x104 spores/ml at 30 DAS and by whorl drop inoculation of pathogen multiplies on sorghum grains at 40 DAS). Disease severity on test entries was scored at silk drying stage and studies revealed that two inbred lines, viz., NAI-112 and NAI-147, and one hybrid, viz., HQPM-1 were found resistant with pooled disease intensity of 4.12 per cent, 4.04 per cent and 4.38 per cent, respectively. Four inbred lines, viz., KDM 381 A, KDM 918 A, NAI-152 and NAI-167 were found susceptible with pooled disease intensity of 52.82 per cent, 51.02 per cent, 58.58 per cent and 61.33 per cent, respectively. The remaining genotypes were moderately resistant to moderately susceptible.

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STUDY OF GROWTH, YIELD AND PHYTOSTEROL OF SQUASH (Cucurbita pepo L. AND MEDICAL PUMPKIN (Cucurbita pepo) AND THEIR HYBRID

IRAQI JOURNAL OF AGRICULTURAL SCIENCES ◽

10.36103/ijas.v51i2.995 ◽

2020 ◽

Vol 51 (2) ◽

pp. 675-684

Author(s):

Elias & et al.

Keyword(s):

Cucurbita Pepo ◽

Block Design ◽

Growth Yield ◽

Hybrid Vigor ◽

College Of Agriculture ◽

Fall Season ◽

Randomized Block Design ◽

Specific Hybrid ◽

Pure Lines ◽

Landscape Gardening

This study was aimed to investigate,growth,yield and phytosterol of squash (Cucurbita pepo L. , medical pumpkin (Cucurbita pepo subsp.) and thier hybrids.The study included crossing among 15 pure lines of squash and medical pumpkin . pepo var. styriaca during fall season 2013 at greenhouse during spring 2014 the genotypes of the Department of Horticulture and Landscape Gardening, College of Agriculture, University of Baghdad. The genotypes (medical pumpkin ST + 9 parents of squash, crosses with medical pumpkin + 11 specific hybrid) were planted at the open field. The layout of the experiment was completely randomized block design. The results showed significant differences among genotypes in the studied traits. The parents ST, E1, E3, E6, K3 and K7 showed superiority when compared to other parents in vegetative and flowering growth, earliness of maturity, yield and phytosterol. Hybrids ST1 × E, H × ST1, K × ST3, K7 × ST, E6 × ST and MR × ST were significantly superiored to other hybrids in the studied characters and had highest positive and significant hybrid vigor.

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