scholarly journals Microbial quality of industrial and retail market grated parmesan cheese in the State of São Paulo, Brazil

2016 ◽  
Vol 46 (12) ◽  
pp. 2257-2263 ◽  
Author(s):  
Carlos Eduardo Gamero Aguilar ◽  
Oswaldo Durival Rossi Junior ◽  
Ana Maria Centola Vidal ◽  
Laryssa Freitas Ribeiro ◽  
Gabriel Augusto Marques Rossi
Keyword(s):  
Microbiological Quality ◽  
Salmonella Spp ◽  
The Public ◽  
Retail Business ◽  
Colony Forming Units ◽  
Parmesan Cheese ◽  
The Moment ◽  
Pathogenic Agents ◽  
Staphylococcus Spp ◽  
Sanitary Conditions

ABSTRACT: With the objective to evaluate the hygienic and sanitary conditions of grated parmesan cheese acquired from the retail business, total 120 cheese samples were acquired: 60 of which were obtained from four different brands of cheese that were grated in factories, and the other 60 samples were obtained from another four brands of cheese that are normally acquired by retailers (supermarkets) in blocks and grated at the moment of sale. The population of heterotrophic mesophilic microorganisms ranged from 1.2×10³ to 1.1×107 colony-forming units (CFU)·g-1. All samples analyzed contained Staphylococcus spp. with populations varying from 1.2×10³ to 8.7×106CFU·g-1, from which 60.0% were classified as coagulase-positive Staphylococcus and 52.5% of the samples possessed populations above the permissible limit set by legislation. Staphylococcus aureus was detected in 57.5% of the samples. Population of molds and yeasts varied from <10 to 1.8×106CFU·g-1. Salmonella spp. was not isolated in this research. A difference was observed between the parmesan cheese grated in factories and that grated in supermarkets, where the former presented better microbiological quality than the latter. Thus, procedures must be proposed to minimize the presence of pathogenic agents reported in grated parmesan cheeses evaluated in the present study because of the public health risk associated with food bacterial contamination.

scholarly journals Microbiological quality of trahira fish (Hoplias malabaricus) from Baixada Maranhense, municipality of São Bento, MA

2018 ◽  
Vol 84 (0) ◽  
Author(s):  
Lucélia Guimarães ◽  
Ana Clara dos Santos ◽  
Elka Ferreira ◽  
Débora Pereira ◽  
Francisca Costa
Keyword(s):  
Microbiological Quality ◽  
Most Probable Number ◽  
Foodborne Diseases ◽  
Salmonella Spp ◽  
Production Chain ◽  
Probable Number ◽  
E Coli ◽  
Staphylococcus Spp ◽  
Sanitary Conditions

ABSTRACT: Fish are considered rich sources of nutrients. Health care throughout its production chain aims to ensure quality, minimizing the risks of transmission of foodborne diseases. In order to evaluate the microbiological quality of trahira fish (H. malabaricus), 40 samples were analysed for Most Probable Number (MPN) of coliforms at 45ºC, counts of aerobic mesophilic bacteria and Staphylococcus spp., identification of E. coli, Salmonella spp. and Aeromonas spp.. Analyses were conducted according to official methods, procedures, and recommendations. Microbiological results showed coliform values at 45 °C ranging from <3 to > 1.1 × 103 MPN/g, presence of E. coli in 14 (35%) samples, counts of mesophilic aerobic bacteria from 9 × 102 to 109 CFU/g and absence of coagulase positive Staphylococcus aureus. Salmonella spp. was detected in 2 (5%) samples, which is in disagreement with the standards required by the RDC Nº 12 of January, 2001 (ANVISA) regarding Aeromonas spp. In total, 36 (90%) samples were contaminated, 7 (19.4%) by A. cavie and 29 (80.6%) by A. hidrophila. The results of this research showed unsatisfactory hygienic and sanitary conditions of fish from the municipality of São Bento (MA), exposing consumers to the risk of foodborne diseases.

scholarly journals Influence of cutting room temperature on the microbiological quality of chicken breast meat

2016 ◽  
Vol 68 (3) ◽  
pp. 814-820 ◽  
Author(s):  
A.P.R. Cintra ◽  
M.C.G. Andrade ◽  
M.M. Lazarini ◽  
D.C.S. Assis ◽  
G.R. Silva ◽  
...  
Keyword(s):  
Environmental Contamination ◽  
Room Temperature ◽  
Microbiological Quality ◽  
Chicken Breast ◽  
Breast Meat ◽  
Salmonella Spp ◽  
Staphylococcal Enterotoxins ◽  
Staphylococcus Spp ◽  
Chicken Breast Meat

ABSTRACT The temperature control in the processing room is one of the major factors associated with the production of safe food with a satisfactory microbiological quality. A total of 288 samples of skinless chicken breast meat were placed in a cutting room, subjected to four different temperatures (12ºC, 14ºC, 16ºC and 18ºC) and collected to evaluate the influence of the room temperature on the microbiological quality during the cutting and boning of chicken breasts. Aerobic mesophilic microorganisms were counted to evaluate the environmental contamination. In addition, coliforms at 35ºC and 45ºC and Staphylococcus spp. were counted, and an analysis for the presence of staphylococcal enterotoxins and Salmonella spp. was performed to determine the microbiological quality of the meat. The results showed an increase in environmental contamination (P=0.01) with an increase in room temperature. However, no significant differences (P˃0.05) were observed in the meat cuts regarding the counts of coliforms at 35ºC and 45ºC, the count of Staphylococcus spp. and the presence of Salmonella spp. Moreover, no staphylococcal enterotoxins were detected in the samples analyzed. Thus, despite increasing the environmental contamination, the increase in the cutting room temperature did not affect the microbiological quality of the final product.

Microbiological Quality and Safety of Ready-to-Eat Street-Vended Foods in Johannesburg, South Africa

1999 ◽  
Vol 62 (11) ◽  
pp. 1278-1284 ◽  
Author(s):  
FRANCINA M. MOSUPYE ◽  
ALEXANDER von HOLY
Keyword(s):  
South Africa ◽  
Foodborne Pathogens ◽  
Water Samples ◽  
Microbiological Quality ◽  
Food Samples ◽  
Quality And Safety ◽  
Salmonella Spp ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Fifty-one ready-to-eat street foods, 18 dishwater, and 18 surface swab samples were collected from six vendors in Johannesburg, South Africa. Food temperatures were recorded at the time of sampling. Standard methods were used to determine aerobic plate counts (APCs), spore counts (SCs), and Enterobacteriaceae counts (ECs) for food samples as well as coliform counts (CCs) for water and swab samples. In addition, Petrifilm Escherichia coli count (PC) plates were used for the enumeration of coliforms in food, water, and swab samples. The presence of selected foodborne pathogens in the food samples as well as the presence of nonpathogenic E. coli 1 (in food and water samples) was also tested for. Predominant colonies isolated from APC plates were characterized to the genus level. Holding temperatures for cooked meats and gravies ranged from 42.0 to 94.0°C, and those for uncooked salads ranged from 29.0 to 39.0°C. Mean APC values of 3.4 (±0.4) log CFU/g, 4.0 (±1.2) log CFU/ml, and 2.1 (±0.4) log CFU/25 cm2 were obtained for food, water, and swab samples, respectively. Mean SC values of 1.6 (±0.2) log CFU/g and 1.5 (±0.3) log CFU/25 cm2 were obtained for food and swab samples, respectively. A mean EC value of 2.0 (±0.4) log CFU/g for food samples and mean CC values of 2.5 (±0.3) log CFU/ml and 1.3 (±0.3) log CFU/25 cm2 for water and swab samples, respectively, were determined. Mean PC values of 1.6 (±0.1) log CFU/g, 1.9 (±0.6) log CFU/ml, and 1.4 (±0.4) log CFU/25 cm2 were determined for food, water, and swab samples, respectively. Bacillus cereus was detected in 22%, Clostridium perfringens in 16%, Salmonella spp. in 2%, and E. coli (non-O157:H+) in 2% of the 51 food samples. E. coli was found in 14 water samples (78%) and in 3 food samples (6%). Campylobacter spp., Listeria monocytogenes, Staphylococcus aureus, Vibrio cholerae, and Yersinia enterocolitica were also tested for in the food samples, but they were not detected. The 340 isolates obtained from APC plates for food, water, and swab samples were predominantly Bacillus spp., Micrococcus spp., and Staphylococcus spp. for all three sample types. It was concluded that the foods analyzed in this study were of acceptable quality and safety.

scholarly journals Microbiological lettuce quality evaluation from traditional, organic and hydroponic growing process

2016 ◽  
Vol 7 (3) ◽  
pp. 82
Author(s):  
Greici Bergamo ◽  
Eliezer Avila Gandra
Keyword(s):  
Escherichia Coli ◽  
Microbiological Quality ◽  
Significant Relation ◽  
Salmonella Spp ◽  
Total Coliforms ◽  
Hygiene Practices ◽  
Lactuca Sativa L ◽  
The Moment ◽  
Good Hygiene

Objective of the study: to evaluate the microbiological quality of lettuce (Lactuca sativa L.) in three different cultivation: organic, hydroponic and traditional. Sixty lettuce samples commercialized in Videira – SC were analyzed. Microorganisms investigated: Salmonella spp., Listeria spp., total coliforms e Escherichia coli. The results showed the absence of Salmonella spp. for all samples. Present of Listeria spp. was found in 16,7% of the samples. Total coliforms e Escherichia coli count varied from <1x101 - 2,8x105 and <1x101 - 1x103, respectively. Traditional and hydroponic farming lettuce samples presented 100% of total coliforms. Escherichia coli analysis occurred in 22,2% of the samples traditional farming, 11,1% hydroponic farming and none in organic farming vegetables. Statistically, we found no significant relation between microbiological contamination and king of growing used in the vegetables production. The strains of the Listeria, total coliforms and Escherichia coli found in the samples indicated hygienic and sanitary quality and so it is important that good hygiene practices are taken from the manufacturing process until the moment of consumption.

Detection and Antibiogram of Different Bacterial Agents From Market Goat Meat

2021 ◽  
Vol 8 (1) ◽  
pp. 135-143
Author(s):  
Md Kamrul Hassan ◽  
Lubna Jahan ◽  
Papia Sultana ◽  
Alamgir Hasan ◽  
Mahbubul Pratik Siddique
Keyword(s):  
Diffusion Method ◽  
Meat Processing ◽  
Salmonella Spp ◽  
Safety Assurance ◽  
Body Regions ◽  
Antibiotic Susceptibility Test ◽  
Spoilage Bacteria ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Sanitary Conditions

The study was conducted to detect various pathogenic as well as spoilage bacteria present in goat meats and also their antibiogram. Different body regions such as brisket, neck and thigh, supplied at different places like Krishi market, Mohakhali kacha bazar, and Charulata market within Dhaka City Corporation area, were taken and the bacteria were isolated. The isolated bacteria were identified on the basis of cultural, morphological, and biochemical characteristics. The isolated bacterial agents were: Staphylococcus spp., Escherichia coli, Bacillus spp., Micrococcus spp., Streptococcus spp., Enterobacter spp., Proteus spp., Citrobacter spp., Salmonella spp., and Pseudomonas spp. Antibiotic susceptibility test using disc diffusion method revealed that most of the isolates were found to be more or less resistant to tetracycline, ampicillin and erythromycin indicating that these may be a threat to consumer’s health. It is emphasized that the present sanitary conditions, as revealed through this study, of meat processing need to be improved so as to minimize microbial contamination. Suggestions are made to introduce Longitudinally Integrated Safety Assurance (LISA) system and Good Manufacturing Practices (GMP) principles. Res. Agric., Livest. Fish.8(1): 135-143, April 2021

scholarly journals Microbiological quality of honey from stingless bee, jandaíra (Melipona subnitida), from the semiarid region of Brazil

2018 ◽  
Vol 48 (9) ◽  
Author(s):  
Carolina de Gouveia Mendes da Escóssia Pinheiro ◽  
Maria Rociene Abrantes ◽  
Rodrigo Otávio Silveira Silva ◽  
Carlos Augusto Oliveira Junior ◽  
Francisco Carlos Faria Lobato ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Microbiological Quality ◽  
Stingless Bee ◽  
Semiarid Region ◽  
Salmonella Spp ◽  
Mesophilic Bacteria ◽  
Melipona Subnitida ◽  
Bacillus Spp ◽  
Staphylococcus Spp

ABSTRACT: The aim of this study was to evaluate the presence of microorganisms in honey produced by the stingless bee (SB) jandaíra (Melipona subnitida) from the semiarid region of Brazil. Thirty-five samples were analyzed and all of them were positive for mesophilic bacteria, coliforms at 45°C, fungi, and yeast. Staphylococcus spp. were identified in 85.7%, while Bacillus spp. were detected in 34.3% of honey samples. DNAs of Clostridium perfringens and C. botulinum were detected in 40% and 2.8% of the samples, respectively. Salmonella spp. and C. difficile were not detected. The present research revealed a great diversity of microorganisms in honey produced by jandaíra.

scholarly journals Evaluation of microbial contents of table eggs at retail outlets in Sokoto metropolis, Nigeria

1970 ◽  
Vol 13 (1) ◽  
pp. 22-28
Author(s):  
MD Salihu ◽  
B Garba ◽  
Y Isah
Keyword(s):  
Salmonella Spp ◽  
Green Wood ◽  
The Public ◽  
Retail Outlets ◽  
Shigella Spp ◽  
Bacillus Spp ◽  
Egg Shells ◽  
Staphylococcus Spp ◽  
Chicken Eggs ◽  
Good Hygiene

This study was carried out to evaluate the microbial contents of chicken eggs, sold at retail outlets in Sokoto metropolis. A total of 160 eggs were collected from 16 randomly selected retail outlets, in Sokoto metropolis, for microbial evaluation. Samples were cultured and isolated using nutrient and McConkey agar for bacteria while Sabauroud dextrose agar was employed for fungus and identified using Harvey and Green Wood method. All the 160 (100%) samples were positive for bacteria (nine different genera); while 104 (65%) egg shells were positive for fungi isolation from the genus Aspergillus; however, evaluation of the egg contents revealed 95(59.4%) positive for bacteria isolations from seven different genera and 86(53.8%) positive for fungi isolations from only one genus Aspergillus. The bacterial genera include Escherichia coli, Salmonella spp, Shigella spp, Corynebacteria, Proteus spp Bacillus spp Staphylococcus spp Streptococcus spp and Klebsiella. The only fungal genus was Aspergillus, which were identified to be Aspergillus flavus, Aspergillus niger and Aspergillus fumigatus. The eggs from these areas should therefore be taken with caution and the public should be educated on the dangers associated with consumption of raw and under cooked eggs and egg products, retailers should be encourage to store their eggs in refrigerators and practice good hygiene in order to prevent microbial growth on the eggs.Keywords: Bacterial isolation, Microbial contents, Retail outlets, Sokoto, Table eggs.

scholarly journals Assessment of the Microbiological Quality of Ready-To Fry Frozen Chicken-Based Snack Items Sold in Bangladesh

2021 ◽  
pp. 52-56
Author(s):  
Anamika Chakrabarty ◽  
Tamanna Islam ◽  
Saurab Kishore Munshi
Keyword(s):  
Foodborne Pathogens ◽  
Microbiological Quality ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Microbiological Analysis ◽  
Chicken Nuggets ◽  
Salmonella Spp ◽  
Viable Bacteria ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp

Microorganisms that are capable of spoiling the product during chill storage as well as several foodborne pathogens can be present in frozen snacks. The present study attempted to investigate the presence of microbial contaminants along with their antibiotic resistance pattern in frozen snacks. In this regard, a total of 15 ready-to-fry frozen chicken-based snack items including three each of chicken pops, chicken samosa, chicken lemongrass lollypop, chicken nuggets and chicken meatball were collected from super shops of Dhaka, Bangladesh. Microbiological analysis revealed that all the samples were highly contaminated with total viable bacteria and fungi in an average of 6 and 4 log cfu/g, respectively which exceeded the standard microbial limit. Klebsiella spp., Staphylococcus spp. and Pseudomonas spp. were encountered in all samples. E. coli, Vibrio spp. and Salmonella spp. were also found in a majority of the samples. Besides, all the isolates were found to be multidrug-resistant. The isolates showed almost 100% resistance against Cefuroxime and Cefixime. A higher proportion of resistance was also reported against Vancomycin and Azithromycin. Whereas, Gentamycin, Ceftriaxone, Colistin and Levofloxacin sensitivity were found in all the isolates. Such chicken-based frozen snack items contaminated with multi-drug resistant microorganisms could be potential vehicles for transmitting food-borne diseases.

scholarly journals Assessment of the Safety of ‘Suya’ Condiment Vended in Three Communities in Rivers State, Nigeria

2021 ◽  
pp. 1-6
Author(s):  
Onoriode C. Eruteya ◽  
El-Praise J. Nmehielle
Keyword(s):  
Microbiological Quality ◽  
Microwave Oven ◽  
Microbial Inactivation ◽  
Microbial Load ◽  
Salmonella Spp ◽  
Randomized Design ◽  
Bacillus Spp ◽  
Processing And Storage ◽  
Rivers State ◽  
Staphylococcus Spp

Aims: The essence of this research was to assess the microbiological quality of suya condiments/spices sold at different points in Obio/Akpor (Choba and Rumuepirikom) and Ikwerre (Aluu) Local Government Areas, Rivers State and reduction of microbial load using home microwave oven. Study Design: Completely randomized design with two replications and average values calculated. Place and Duration of Study: Food and Industrial Microbiology Laboratory, University of Port and Emadavistic Medical and Research Laboratory, Osaks House, East-West road Port Harcourt for three months. Methodology: Twelve (12) samples of already formulated suya condiments/spices were sampled from twelve different suya vendors at Choba, Rumuepirikom, and Aluu. Standard methods were used for the microbiological analyses and microbial inactivation using home microwave oven. Results: The total plate counts (log10 CFU/g) for the samples ranged from 5.35 to 6.29, 5.67 to 6.09, and 5.88 to 6.36 for samples collected from Choba, Rumuepirikom and Aluu respectively. The isolated bacteria on the basis of cultural, physiological and biochemical characteristics were identified as: Staphylococcus spp. (44.4%), Salmonella spp. (11.1%), Bacillus spp. (35.2%), Shigella sp. (1.9%), Enterobacter sp. (3.7%) and Streptococcus sp. (3.7%) from all three locations. A decrease in the microbial load was noted as exposure time increased from 0 to 60 s. (exposure to what) Conclusion: In all, suya condiments collected for this study were contaminated with bacteria of public health significance which suggest the need for microbial inactivation and an improvement in hygiene practice during processing and storage.

Evaluation of hygienic conditions of food contact surfaces in a hospital kitchen in Morocco

2020 ◽  
Author(s):  
Ghita Benjelloun Touimi ◽  
Laila Bennani ◽  
Sanae Berrada ◽  
Moussa Benboubker ◽  
Bahia Bennani
Keyword(s):  
Microbiological Quality ◽  
Salmonella Spp ◽  
Coagulase Negative Staphylococci ◽  
Food Contact ◽  
Healthcare Settings ◽  
Food Contact Surfaces ◽  
Haccp System ◽  
Sanitary Conditions ◽  
Cutting Boards ◽  
Hygienic Conditions

Background and Objectives: Food in healthcare settings are complementary to medical treatment, hence it should be produced in good sanitary conditions. In fact, hospitalized and immune-compromised patients are more likely to have foodborne infections than the rest of the community. The aim of our study is to evaluate the microbiological quality of food contact surfaces in a hospital kitchen in Morocco. Materials and Methods: A total of 238 samples was collected from kitchen surfaces and analyzed for total aerobic mesophilic bacteria (AMC), Enterobacteriaceae and Staphylococcus aureus count and the presence of Salmonella spp., Pseudomonas spp. and Listeria monocytogenes. Results: The bacteriological analysis shows that the highest rates of compliance with good hygienic conditions were obtained in baking worktops (77%) and serving meal worktops (50%) and the vegetables cutting boards (45.83%). In contrary, some surfaces show a low level of compliance, such as the raw meat cutting boards (96%). The isolated bacteria were S. aureus, coagulase-negative staphylococci, Escherichia coli, Serratia marcescens, Serratia odorifera, Raoultela ornithiaolytica and Pseudomonas aeroguinosa. Conclusion: The actual results indicate that the high levels of bacterial counts on kitchen surfaces, presents an evident need to improve the hygienic process and adopt an HACCP system in this facility.

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