Detection and Antibiogram of Different Bacterial Agents From Market Goat Meat

2021 ◽  
Vol 8 (1) ◽  
pp. 135-143
Author(s):  
Md Kamrul Hassan ◽  
Lubna Jahan ◽  
Papia Sultana ◽  
Alamgir Hasan ◽  
Mahbubul Pratik Siddique
Keyword(s):  
Diffusion Method ◽  
Meat Processing ◽  
Salmonella Spp ◽  
Safety Assurance ◽  
Body Regions ◽  
Antibiotic Susceptibility Test ◽  
Spoilage Bacteria ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Sanitary Conditions

The study was conducted to detect various pathogenic as well as spoilage bacteria present in goat meats and also their antibiogram. Different body regions such as brisket, neck and thigh, supplied at different places like Krishi market, Mohakhali kacha bazar, and Charulata market within Dhaka City Corporation area, were taken and the bacteria were isolated. The isolated bacteria were identified on the basis of cultural, morphological, and biochemical characteristics. The isolated bacterial agents were: Staphylococcus spp., Escherichia coli, Bacillus spp., Micrococcus spp., Streptococcus spp., Enterobacter spp., Proteus spp., Citrobacter spp., Salmonella spp., and Pseudomonas spp. Antibiotic susceptibility test using disc diffusion method revealed that most of the isolates were found to be more or less resistant to tetracycline, ampicillin and erythromycin indicating that these may be a threat to consumer’s health. It is emphasized that the present sanitary conditions, as revealed through this study, of meat processing need to be improved so as to minimize microbial contamination. Suggestions are made to introduce Longitudinally Integrated Safety Assurance (LISA) system and Good Manufacturing Practices (GMP) principles. Res. Agric., Livest. Fish.8(1): 135-143, April 2021

scholarly journals Evaluation of microbial contamination level and the drug susceptible pattern of the isolates cultivated from famous dessert food

Food Research ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 426-430
Author(s):  
T. Akter ◽  
T. Ishma ◽  
S.R. Razzeb ◽  
S.H.M. Uddin ◽  
M. Islam ◽  
...  
Keyword(s):  
Microbial Contamination ◽  
Heterotrophic Bacteria ◽  
Ice Cream ◽  
Drug Susceptibility ◽  
Diffusion Method ◽  
Contamination Level ◽  
Salmonella Spp ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Present study endeavored to evaluate the microbial contamination level along with their drug resistant pattern in some popular desert food items collected from different food shops in Dhaka city, Bangladesh. The microbial evaluation was conducted through conventional cultural methods and drug susceptibility test was executed through disc diffusion method. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 105 CFU/g. In case of specific microflora, the growth of Staphylococcus spp. was very high in sweet, faluda, milk cake and ice cream as estimated up to 105 CFU/g, halua and sweet yogurt showed 104 CFU/g while rest of the samples revealed 103 CFU/g. E. coli was found only in faluda and ice cream up to 103 CFU/g whereas Klebsiella spp. was estimated in all the samples within the range of 102 CFU/g to 105 CFU/g. Salmonella spp., Pseudomonas spp. and Bacillus spp. were totally absent in all the samples. Most of the isolates were found to be resistant against most of the antibiotics. Meanwhile, streptomycin (10 µg), gentamicin (10 µg), azithromycin (15 µg), and nalidixic acid (5 µg) were effective drug against both E. coli and Staphylococcus spp.

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

Microbiological Quality and Safety of Ready-to-Eat Street-Vended Foods in Johannesburg, South Africa

1999 ◽  
Vol 62 (11) ◽  
pp. 1278-1284 ◽  
Author(s):  
FRANCINA M. MOSUPYE ◽  
ALEXANDER von HOLY
Keyword(s):  
South Africa ◽  
Foodborne Pathogens ◽  
Water Samples ◽  
Microbiological Quality ◽  
Food Samples ◽  
Quality And Safety ◽  
Salmonella Spp ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Fifty-one ready-to-eat street foods, 18 dishwater, and 18 surface swab samples were collected from six vendors in Johannesburg, South Africa. Food temperatures were recorded at the time of sampling. Standard methods were used to determine aerobic plate counts (APCs), spore counts (SCs), and Enterobacteriaceae counts (ECs) for food samples as well as coliform counts (CCs) for water and swab samples. In addition, Petrifilm Escherichia coli count (PC) plates were used for the enumeration of coliforms in food, water, and swab samples. The presence of selected foodborne pathogens in the food samples as well as the presence of nonpathogenic E. coli 1 (in food and water samples) was also tested for. Predominant colonies isolated from APC plates were characterized to the genus level. Holding temperatures for cooked meats and gravies ranged from 42.0 to 94.0°C, and those for uncooked salads ranged from 29.0 to 39.0°C. Mean APC values of 3.4 (±0.4) log CFU/g, 4.0 (±1.2) log CFU/ml, and 2.1 (±0.4) log CFU/25 cm2 were obtained for food, water, and swab samples, respectively. Mean SC values of 1.6 (±0.2) log CFU/g and 1.5 (±0.3) log CFU/25 cm2 were obtained for food and swab samples, respectively. A mean EC value of 2.0 (±0.4) log CFU/g for food samples and mean CC values of 2.5 (±0.3) log CFU/ml and 1.3 (±0.3) log CFU/25 cm2 for water and swab samples, respectively, were determined. Mean PC values of 1.6 (±0.1) log CFU/g, 1.9 (±0.6) log CFU/ml, and 1.4 (±0.4) log CFU/25 cm2 were determined for food, water, and swab samples, respectively. Bacillus cereus was detected in 22%, Clostridium perfringens in 16%, Salmonella spp. in 2%, and E. coli (non-O157:H+) in 2% of the 51 food samples. E. coli was found in 14 water samples (78%) and in 3 food samples (6%). Campylobacter spp., Listeria monocytogenes, Staphylococcus aureus, Vibrio cholerae, and Yersinia enterocolitica were also tested for in the food samples, but they were not detected. The 340 isolates obtained from APC plates for food, water, and swab samples were predominantly Bacillus spp., Micrococcus spp., and Staphylococcus spp. for all three sample types. It was concluded that the foods analyzed in this study were of acceptable quality and safety.

scholarly journals Ciprofloxacin susceptibility of Salmonella enteric serovar Typhi and Paratyphi A from blood samples of suspected enteric fever patients

2012 ◽  
Vol 1 (1) ◽  
pp. 9-13 ◽  
Author(s):  
D Adhikari ◽  
D Acharya ◽  
P Shrestha ◽  
R Amatya
Keyword(s):  
Salmonella Enterica ◽  
Enteric Fever ◽  
Diffusion Method ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Disc Diffusion ◽  
Salmonella Spp ◽  
Blood Samples ◽  
Antibiotic Susceptibility Test

INTRODUCTION: Enteric fever caused by Salmonella enterica serovars Typhi and Paratyphi A is the common clinical diagnosis among febrile patients presenting to hospital in Nepal. The aim of this study was to evaluate the ciprofloxacin (CIP) and other antibiotics susceptibility patterns of Salmonella enterica serovars Typhi and Paratyphi A from blood samples of suspected enteric fever patients visiting KIST Medical College, Kathmandu. MATERIALS AND METHODS: Antibiotic susceptibility test was performed by Kirby Bauer disc diffusion method as per CLSI guidelines whereas MIC determination of ciprofloxacin was performed by agar dilution method. RESULTS: Altogether 64 presumptive Salmonella spp. were isolated from 840 blood samples of suspected enteric fever patients, of which 41 were S. Typhi and 23 were S. Paratyphi A. All Salmonella spp. isolates were sensitive to ceftriaxone and ofloxacin whereas 4 isolates were resistant to ciprofloxacin. One isolates each of S. Typhi and S. Paratyphi A were multidrug resistant. Fifty five isolates of Salmonella spp. were resistant to nalidixic acid (NA) with higher rate in S. Paratyphi A (91.3%) compared to S. Typhi (82.9%). MIC determination of ciprofloxacin revealed that majority of the isolates were resistant to ciprofloxacin and 2 isolates were resistant with MIC value of 4 µg/ml and 8 µg/ml. Importantly, we found simultaneous presence of NA resistance and decreased susceptibility to CIP suggesting that resistance to NA is a reliable indicator of decreased CIP susceptibility. CONCLUSIONS: Disc diffusion test failed to detect the reduced susceptibility of ciprofloxacin. Hence the MIC determination of ciprofloxacin against Salmonella spp. isolates would be important.  DOI: http://dx.doi.org/10.3126/ijim.v1i1.6938Int J Infect Microbiol 2012;1(1):9-13

scholarly journals Influence of tannery wastes and the surrounding environment of tannery industries on microbial growth and proliferation

2017 ◽  
Vol 7 (1) ◽  
pp. 14-18
Author(s):  
Nayan Chandra Das ◽  
Farzana Hossaini ◽  
Saurab Kishore Munshi
Keyword(s):  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Tannery Waste ◽  
Surrounding Environment ◽  
Bacillus Spp ◽  
Tannery Wastes ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

The present study was carried out to assess the degree of microbiological proliferation in tannery wastes and the surrounding environment of the tannery industries. In this regard, a total of 8 tannery waste (n=4) and environmental (n=4) samples were tested. All the samples contained a huge load of bacteria and fungi in an average of 108 cfu/g or ml. An extended numbers of pathogenic bacteria were recovered. Among the pathogenic bacteria, Staphylococcus spp. was predominant. Most of the samples exhibited the presence of Pseudomonas spp. Salmonella spp. and fecal coliform were found each in one sample. Bacillus spp., Escherichia coli, Klebsiella spp. and Vibrio spp. were found in few samples. The average load of the pathogens was 104 cfu/g or ml. All the pathogenic isolates were found to be multidrug resistant. Higher resistance was found against penicillin and streptomycin. Tannery waste after lather treatment sample showed antibacterial activity against all the pathogens tested. Overall, presence of pathogenic microorganisms with multidrug resistance traits may pose serious public health threats. Stamford Journal of Microbiology, Vol.7(1) 2017: 14-18

scholarly journals ISOLATION, IDENTIFICATION AND ANTIBIOTIC RESISTANCE OF AEROMONAS SPP. AND SALMONELLA SPP. FROM THE FRESH WATER LOACH, LEPIDOCEPHALICHTHYS GUNTEA AND WATER OF TERAI RIVER LOTCHKA, WEST BENGAL, INDIA

2013 ◽  
Vol 58 (1-2) ◽  
pp. 5-17 ◽  
Author(s):  
Rudra Prasad Roy ◽  
Min Bahadur ◽  
Sudip Barat
Keyword(s):  
Antibiotic Resistance ◽  
Fresh Water ◽  
Pathogenic Bacteria ◽  
West Bengal ◽  
Penicillin G ◽  
Diffusion Method ◽  
Body Parts ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Antibiotic Susceptibility Test

Abstract An investigation was conducted to identify the bacterial isolates and to study the antibiotic resistance patterns of Aeromonas spp. and Salmonella spp. from the skin, gills, and gut of the fresh water loach, Lepidocephalichthys guntea (HAMILTON BUCHANAN) and water sampled from four different sites along the River Lotchka in Darjeeling District, West Bengal, India. Isolated bacteria were identified by different biochemical procedures, and Polymerase Chain Reaction was performed using genus specific 16S rDNA primers for confirmation of identification of Aeromonas spp. and Salmonella spp. Antibiotic susceptibility test of bacterial isolates was also done by Disc Diffusion method. A total of 49 Aeromonas spp. and 24 Salmonella spp. were isolated from tested samples. Maximum resistance was exhibited for Penicillin-G, Ampicillin, and Cephalothin (>75% resistant Aeromonas spp. and >60% Salmonella spp. in the four different sites of river and body parts of fish). Ciprofloxacin and Tetracycline resistance was almost nil. The results indicated that the river water and resident fish were contaminated with multi-antibiotic resistant enteric pathogenic bacteria. This study, thus, provides valuable information for making policy decisions aimed at reducing microbial contamination of fish and the indiscriminate use of antibiotics.

scholarly journals Exploring Antibiotic Resistance Pattern of Escherichia coli, Salmonella spp., and Staphylococcus spp. Isolated from Eggs in Rajshahi

2021 ◽  
Vol 3 (4) ◽  
pp. 25-30
Author(s):  
Md Hakimul Haque ◽  
Md. Mizanur Rahman ◽  
Md. Lovelu Miah ◽  
Soshe Ahmed ◽  
Md. Rabiul Islam Sazib ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Bacterial Pathogen ◽  
Diffusion Method ◽  
Resistance Pattern ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Disk Diffusion Method ◽  
E Coli ◽  
Antibiotic Resistance Pattern ◽  
Staphylococcus Spp

Chicken eggs are a major component of people’s diets, with an average yearly consumption of approximately 103 eggs per person in Bangladesh. Eggs act as an important carrier of food-borne pathogen worldwide. The study was conducted to identify the prevalence and antibiotic resistance pattern of E. coli, Salmonella spp., and Staphylococcus spp., in eggs isolated from farms and different markets of Rajshahi, Bangladesh. A total of 60 eggs were collected randomly between April to December 2019. The isolation and identification of bacterial pathogen was done in accordance with standard procedures. The bacterial isolates were subjected to antibiotic susceptibility testing against seven commonly used antibiotics using Kirby-Bauer disk diffusion method. An overall prevalence of E. coli, Salmonella spp., and Staphylococcus spp. were found to be 35.0%, 28.33%, and 23.33%, respectively. E. coli were found highly resistant to penicillin (100%), tetracycline (80.95%), ampicillin (100%), and erythromycin (85.71%) and were sensitive to amoxicillin (71.42%), ciprofloxacin (85.71%), and gentamicin (95.23%). Salmonella spp. was highly resistant to penicillin (100%), erythromycin (82.35%) and tetracycline (82.35%), and was sensitive to gentamicin (94.11%), amoxicillin (76.47%) and ciprofloxacin (70.58%). Staphylococcus spp. was resistant to penicillin (100%), erythromycin (78.57%), tetracycline (85.71%), amoxicillin (100%), and ampicillin (100%) but sensitive to ciprofloxacin (85.71%), and gentamicin (92.85%). The higher prevalence of multidrug-resistant (MDR) bacteria can easily enter the food chain, which poses a public health threat.

scholarly journals Bacterial pathogens isolated from currency notes circulating amongst live-bird marketers in Damaturu and Potiskum, Yobe State, Nigeria

2021 ◽  
pp. 25-32
Keyword(s):  
Public Health ◽  
Staphylococcus Aureus ◽  
Antibiotic Susceptibility ◽  
Bacterial Pathogens ◽  
Diffusion Method ◽  
Salmonella Spp ◽  
Bacillus Spp ◽  
Klebsiella Spp ◽  
Live Bird ◽  
Coagulase Positive Staphylococcus

Bacterial contamination of currency notes is of veterinary and public health importance because contaminated notes could serve as vector for the spread of pathogenic and perhaps multidrug resistant bacteria. The aim of this study was to assess the antibiotic susceptibility profile of bacterial contaminants found in various denominations of the Nigerian currency circulating among live-bird vendors in Yobe State, Nigeria. Three hundred and twenty (320) currency notes of all denominations were collected from the marketers for investigation. All samples were screened for bacterial pathogens according to standard techniques. The disc diffusion method was used to assess the antibiotic susceptibility of each of the isolated bacterial species against twelve antimicrobial drugs. The result showed that the higher denominations (N1000 - N100) were contaminated by Bacillus spp, (48.2 %), Eischerchiia coli ((13.5 %), Klebsiella spp (6.4 %) Pseudomonas aeruginosa (5.0%), Salmonella spp (0.7%), Coagulase positive Staphylococcus aureus (17.0%), and Streptococcus spp (9.2%) while the lower denominations (N50 - N5) were contaminated by Bacillus spp (37.1%), coagulase positive Staphylococcus aureus (19.1%,), E. coli (14.6 %), Klebsiella spp (5.1 %), P. aeruginosa (6.7%), Salmonella spp (7.3%) and Streptococcus spp (10.1%). All the isolated bacteria were resistant to ampicillin, oxacillin, amoxicillin, and tetracycline. Ciprofloxacin had the greatest activity followed by nitrofurantoin, neomycin, gentamicin, chloramphenicol and streptomycin. The present study revealed that Naira notes circulating among live-bird marketers were contaminated by pathogenic bacteria. It is recommended that live-bird traders should observe strict personal and environmental hygiene while engaging in their daily transaction to forestall any public health threat that may arise from transmission of disease pathogens from the legal tender of transaction in the market.

scholarly journals Isolation and Identification of Uterine Microorganisms in Postpartum Dairy Cows

2012 ◽  
Vol 28 (1) ◽  
pp. 19-23
Author(s):  
Azizun Nesa ◽  
S D Joy ◽  
M O Faruk
Keyword(s):  
Dairy Cows ◽  
Bacterial Population ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Diseased Animal ◽  
Uterine Infection ◽  
Different Types ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Apparently Healthy

The aim of this study was to isolate and identify uterine microorganisms in postpartum cows and to observe  the occurrence of uterine infection in crossbred and local cows. One hundred and nine samples, from both  crossbred and local dairy cows were collected from Chittagong area. They were inseminated artificially  (n=89) and naturally (n=20). Samples were collected at least 3 months after calving. Among the selected cows,  33 had different reproductive diseases and the rest were from apparently healthy cows. The crossbred dairy  cows were more susceptible to reproductive diseases (19.27%) than the local ones. Uterine infection was  higher (52%) in cows inseminated artificially than that of natural ones (21.21%). Nineteen different types of  bacteria were isolated from both diseased and healthy animals, except Clostridium spp and Nocardia spp which  were not found in diseased animal. Likewise, Bacillus spp and Salmonella spp were absent in healthy animals.  Most prevalent bacteria in diseased animal were Staphylococcus spp and Streptococcus spp found in 33% and  27.3% of the cases respectively. In non-diseased cases, the highest number of samples was positive for  Bacteroied spp (13.2%) followed by Streptococcus spp (10.4%). On average, the highest number of bacterial  population was isolated from diseased samples and most of those bacteria are pathogenic. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11804 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 19-23

scholarly journals Microbiological quality of honey from stingless bee, jandaíra (Melipona subnitida), from the semiarid region of Brazil

2018 ◽  
Vol 48 (9) ◽  
Author(s):  
Carolina de Gouveia Mendes da Escóssia Pinheiro ◽  
Maria Rociene Abrantes ◽  
Rodrigo Otávio Silveira Silva ◽  
Carlos Augusto Oliveira Junior ◽  
Francisco Carlos Faria Lobato ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Microbiological Quality ◽  
Stingless Bee ◽  
Semiarid Region ◽  
Salmonella Spp ◽  
Mesophilic Bacteria ◽  
Melipona Subnitida ◽  
Bacillus Spp ◽  
Staphylococcus Spp

ABSTRACT: The aim of this study was to evaluate the presence of microorganisms in honey produced by the stingless bee (SB) jandaíra (Melipona subnitida) from the semiarid region of Brazil. Thirty-five samples were analyzed and all of them were positive for mesophilic bacteria, coliforms at 45°C, fungi, and yeast. Staphylococcus spp. were identified in 85.7%, while Bacillus spp. were detected in 34.3% of honey samples. DNAs of Clostridium perfringens and C. botulinum were detected in 40% and 2.8% of the samples, respectively. Salmonella spp. and C. difficile were not detected. The present research revealed a great diversity of microorganisms in honey produced by jandaíra.

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