scholarly journals Evaluation of microbial contents of table eggs at retail outlets in Sokoto metropolis, Nigeria

1970 ◽  
Vol 13 (1) ◽  
pp. 22-28
Author(s):  
MD Salihu ◽  
B Garba ◽  
Y Isah
Keyword(s):  
Salmonella Spp ◽  
Green Wood ◽  
The Public ◽  
Retail Outlets ◽  
Shigella Spp ◽  
Bacillus Spp ◽  
Egg Shells ◽  
Staphylococcus Spp ◽  
Chicken Eggs ◽  
Good Hygiene

This study was carried out to evaluate the microbial contents of chicken eggs, sold at retail outlets in Sokoto metropolis. A total of 160 eggs were collected from 16 randomly selected retail outlets, in Sokoto metropolis, for microbial evaluation. Samples were cultured and isolated using nutrient and McConkey agar for bacteria while Sabauroud dextrose agar was employed for fungus and identified using Harvey and Green Wood method. All the 160 (100%) samples were positive for bacteria (nine different genera); while 104 (65%) egg shells were positive for fungi isolation from the genus Aspergillus; however, evaluation of the egg contents revealed 95(59.4%) positive for bacteria isolations from seven different genera and 86(53.8%) positive for fungi isolations from only one genus Aspergillus. The bacterial genera include Escherichia coli, Salmonella spp, Shigella spp, Corynebacteria, Proteus spp Bacillus spp Staphylococcus spp Streptococcus spp and Klebsiella. The only fungal genus was Aspergillus, which were identified to be Aspergillus flavus, Aspergillus niger and Aspergillus fumigatus. The eggs from these areas should therefore be taken with caution and the public should be educated on the dangers associated with consumption of raw and under cooked eggs and egg products, retailers should be encourage to store their eggs in refrigerators and practice good hygiene in order to prevent microbial growth on the eggs.Keywords: Bacterial isolation, Microbial contents, Retail outlets, Sokoto, Table eggs.

scholarly journals Isolation of Multi-Drug Resistant Potential Pathogenic Bacteria From Blow Fly Collected From Different Areas of Dhaka City

2021 ◽  
Vol 49 (2) ◽  
pp. 205-214
Author(s):  
Tangin Akter ◽  
Samira Jahan ◽  
Sangita Ahmed ◽  
Shanjida Sultana ◽  
Shefali Begum
Keyword(s):  
Pathogenic Bacteria ◽  
Dhaka City ◽  
Blow Fly ◽  
Salmonella Spp ◽  
Blow Flies ◽  
Fish Market ◽  
Shigella Spp ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

The research work was conducted for isolation and identification of potential pathogenic bacteria from blow fly (Calliphora spp.) and to investigate their antibiotic susceptibility. For this purpose, 20 blow flies were collected from 4 selected areas (Curzon Hall, Dhaka Medical College Hospital, Fruit Store and Fish Market) of Dhaka city. A total of 40 samples (20 from external surface and 20 from alimentary gut homogenates) were prepared from the blow flies. Among the total 20 flies, 3(15%) were collected from Curzon Hall and 5(12.5%) isolates were obtained from them; 6(30%) flies were collected from DMCH which had 11(27.5%) isolates; 5(25%) flies were collected from fruit stores and 10(25%) isolates were collected from there; 6(30%) flies were collected from fish market and 14(35%) isolates were collected from there. The isolated bacteria were presumptively identified as Escherichia coli, 12 (30%); Salmonella spp., 8 (20%); Shigella spp., 4(10%); Enterobacter spp., 3 (7.5%); Klebsiella spp., 2 (5%); Bacillus spp., 7 (17.5%) and Staphylococcus spp., 4 (10%) in number which were based on morphology as observed under microscope as well as cultural and biochemical properties. All of these isolates were resistant to various antibiotics. Bacterial susceptibility showed that E. coli isolates were highly resistant (66.6%) to Penicillin, Salmonella spp. mostly resistant (62.5%) to Penicillin and Tetracycline. Klebsiella spp. Isolates were 100% resistant to Penicillin and 100% sensitive to Ciprofloxacin, Imipenem and Tetracycline. In case of Shigella spp. and Enterobacter spp., 75% and 100% isolates were resistant to Penicillin respectively. Among the Staphylococcus spp. isolates, 75% were resistant to Ampicillin and Penicillin. In case of Bacillus spp. isolates, 57.2% were resistant to Penicillin and 100% sensitive to Imipenem. Antibiotic resistance of these species affect our human health also. This study demonstrated the potential of blow flies as a vector of various pathogenic microorganisms and a mode of transmission of antibiotic resistance. Bangladesh J. Zool. 49 (2): 205-214, 2021

scholarly journals Evaluation of sanitary quality of goat milk in dairy industries from the Cariri region, state of Paraíba

2021 ◽  
Vol 10 (12) ◽  
pp. e596101220735
Author(s):  
Iara Nunes de Siqueira ◽  
Aline Antas Cordeiro Cavalcanti ◽  
Joyce Galvão de Souza ◽  
Filipe Jordão Pereira de Medeiros ◽  
João Carlos Taveira ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Listeria Monocytogenes ◽  
Pathogenic Bacteria ◽  
Goat Milk ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Enterococcus Spp ◽  
Bacillus Spp ◽  
Thermotolerant Coliforms ◽  
Staphylococcus Spp

The sanitary evaluation of equipment and hands is fundamental to investigate the presence of pathogens in the dairy industry. Then, this study aims to evaluate the sanitization of equipment, workers’ hands, raw and pasteurized milk in goat milk dairies in the Cariri region, state of Paraíba.  Collected 32 samples of four dairies represented by letters A, B, C, and D. The followings contents were analyzed: mesophiles, total and thermotolerant coliforms, Escherichia coli, Staphylococcus aureus, Samonella spp. and Listeria monocytogenes in the reception tank, pasteurization tank, packing machine, package, wall, workers’ hand, and each dairy’s raw and pasteurized milk. After isolation, 84 colonies were confirmed by MALDI TOF. The indicator microorganisms presented variations for the workers’ hands, while A and B stayed within the patterns. For the equipment, only dairy B was within limits. They were out of the standard for mesophiles, total coliforms, and thermotolerant regarding raw and pasteurized milk. The microorganisms, the Enterobacteriaceae family presented a higher frequency, with 77.38%, and within this family, Escherichia coli, Klebsiella spp., and Enterobacter spp. were the most prevalent. Gram-positive corresponded to 22.62%, Bacillus spp., Staphylococcus spp., Enterococcus spp., and Macrococcus caseolyticus. Listeria monocytogenes and Salmonella spp. were not isolated. These demonstrate failures in goat milk processing with pathogenic bacteria in several dairy plants, indicating the need to adjust the product’s quality control.

Microbiological Quality and Safety of Ready-to-Eat Street-Vended Foods in Johannesburg, South Africa

1999 ◽  
Vol 62 (11) ◽  
pp. 1278-1284 ◽  
Author(s):  
FRANCINA M. MOSUPYE ◽  
ALEXANDER von HOLY
Keyword(s):  
South Africa ◽  
Foodborne Pathogens ◽  
Water Samples ◽  
Microbiological Quality ◽  
Food Samples ◽  
Quality And Safety ◽  
Salmonella Spp ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Fifty-one ready-to-eat street foods, 18 dishwater, and 18 surface swab samples were collected from six vendors in Johannesburg, South Africa. Food temperatures were recorded at the time of sampling. Standard methods were used to determine aerobic plate counts (APCs), spore counts (SCs), and Enterobacteriaceae counts (ECs) for food samples as well as coliform counts (CCs) for water and swab samples. In addition, Petrifilm Escherichia coli count (PC) plates were used for the enumeration of coliforms in food, water, and swab samples. The presence of selected foodborne pathogens in the food samples as well as the presence of nonpathogenic E. coli 1 (in food and water samples) was also tested for. Predominant colonies isolated from APC plates were characterized to the genus level. Holding temperatures for cooked meats and gravies ranged from 42.0 to 94.0°C, and those for uncooked salads ranged from 29.0 to 39.0°C. Mean APC values of 3.4 (±0.4) log CFU/g, 4.0 (±1.2) log CFU/ml, and 2.1 (±0.4) log CFU/25 cm2 were obtained for food, water, and swab samples, respectively. Mean SC values of 1.6 (±0.2) log CFU/g and 1.5 (±0.3) log CFU/25 cm2 were obtained for food and swab samples, respectively. A mean EC value of 2.0 (±0.4) log CFU/g for food samples and mean CC values of 2.5 (±0.3) log CFU/ml and 1.3 (±0.3) log CFU/25 cm2 for water and swab samples, respectively, were determined. Mean PC values of 1.6 (±0.1) log CFU/g, 1.9 (±0.6) log CFU/ml, and 1.4 (±0.4) log CFU/25 cm2 were determined for food, water, and swab samples, respectively. Bacillus cereus was detected in 22%, Clostridium perfringens in 16%, Salmonella spp. in 2%, and E. coli (non-O157:H+) in 2% of the 51 food samples. E. coli was found in 14 water samples (78%) and in 3 food samples (6%). Campylobacter spp., Listeria monocytogenes, Staphylococcus aureus, Vibrio cholerae, and Yersinia enterocolitica were also tested for in the food samples, but they were not detected. The 340 isolates obtained from APC plates for food, water, and swab samples were predominantly Bacillus spp., Micrococcus spp., and Staphylococcus spp. for all three sample types. It was concluded that the foods analyzed in this study were of acceptable quality and safety.

scholarly journals Occurrence of pathogenic microorganisms in dessert items collected from Dhaka city

2020 ◽  
Vol 9 (1) ◽  
pp. 19-22
Author(s):  
Shohana Akter ◽  
Anasua Sarkar ◽  
Kamal Kanta Das
Keyword(s):  
Heterotrophic Bacteria ◽  
Good Health ◽  
Poor Quality ◽  
Dhaka City ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Due to delicious taste and readily availability, desserts are one of the most popular foods in Dhaka city. High amount of carbohydrate and protein in dessert items make them more susceptible to proliferation of microbial growth. Present study depicted a complete microbiological profile of some popular desserts such as, sweet, pastry, ice cream, pudding, falooda, yogurt and custard available in different food shop at Dhaka city, Bangladesh. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 106 cfu/g. In case of specific microflora, the growth of Staphylococcus spp., Klebsiella spp. and Pseudomonas spp. were observed in most of the samples indicates the poor quality of these products. Bioburdens of E. coli in sweet, pudding and yogurt were found in the range of 1.2×102 to 2.7×103 cfu/g. Salmonella spp., Shigella spp. and Vibrio spp. could not be isolated from any of the samples. Current study indicates that hygienic conditions should be maintained during preparation, packaging and retailing of dessert items in order to reduce the load of contamination in ready to eat foods which will ensure good health of consumers. Stamford Journal of Microbiology, Vol.9(1) 2019: 19-22

scholarly journals Identification and antibiogram study of bacteria isolated from different street food

2018 ◽  
Vol 4 (3) ◽  
pp. 279-287
Author(s):  
Mohamed Ahmed Khalif ◽  
Md Khaled Hossain ◽  
Nazmi Ara Rumi ◽  
Md Shajedur Rahman ◽  
Md Aoulad Hosen
Keyword(s):  
Escherichia Coli ◽  
Bacterial Contamination ◽  
Wide Spectrum ◽  
Salmonella Spp ◽  
Street Vendor ◽  
Street Food ◽  
Food Borne ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Food borne diseases are an increasingly recognized problem involving a wide spectrum of illnesses caused by bacterial contamination of food.Microorganism poses potential human health problems and is mainly transmitted through consumption of contaminated foods .Bangladesh is one of the densely populated country where majority of the people consume cheap foods prepared in unlicensed food selling points. The objective of the present study was to investigate the microbiological quality of different street food (Amra, Fusca, Chanachur and Guava) sold by various street vendor at Dinajpur, Bangladesh. A total of 20 samples were collected randomly from street vendors and tested for the presence of bacteria following standard microbiological method used for isolation, enumaration and identification of bacreria. Among the samples all had bacterial contamination. The total viable count (TVC) in diferrent street food samples was ranged from 8.0 × 105 CFU/g to 6.7x107 CFU/g. Among samples Escherichia coli 7(35%), Staphylococcus spp. 6(30%), Klebsiella spp. 5(25%), Salmonella spp. 1(5%) and Shigella spp. 1 (5%) were isolated. Escherichia coli were found highest 3(50%) Amra samples and 2(40%) Guava samples, then another organisms Staphylococcus spp. was found highest 2(50%) Chanachur and 2(40%) Fusca samples and then Klebsiella spp. was found highest 2(40%) Fusca samples. Antibiotic sensitivity test showed that Shigella spp., Staphylococcus spp., Klebsiella spp., Salmonella spp. and Escherichia coli were sensitive to Ciprofloxacin and Salmonella spp. were resistance to Gentamycin. All isolates found resistant to Cefixime, Cefalexin, Erythromycin, Fusidic acid, Cefuroxime and Aztreonam. All of the sample harbor multidrug resistant food borne bacteria which might cause public health hazards if these antibiotic resistance transfer to human.Asian J. Med. Biol. Res. September 2018, 4(3): 279-287

scholarly journals Influence of tannery wastes and the surrounding environment of tannery industries on microbial growth and proliferation

2017 ◽  
Vol 7 (1) ◽  
pp. 14-18
Author(s):  
Nayan Chandra Das ◽  
Farzana Hossaini ◽  
Saurab Kishore Munshi
Keyword(s):  
Pathogenic Bacteria ◽  
Multidrug Resistant ◽  
Salmonella Spp ◽  
Tannery Waste ◽  
Surrounding Environment ◽  
Bacillus Spp ◽  
Tannery Wastes ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

The present study was carried out to assess the degree of microbiological proliferation in tannery wastes and the surrounding environment of the tannery industries. In this regard, a total of 8 tannery waste (n=4) and environmental (n=4) samples were tested. All the samples contained a huge load of bacteria and fungi in an average of 108 cfu/g or ml. An extended numbers of pathogenic bacteria were recovered. Among the pathogenic bacteria, Staphylococcus spp. was predominant. Most of the samples exhibited the presence of Pseudomonas spp. Salmonella spp. and fecal coliform were found each in one sample. Bacillus spp., Escherichia coli, Klebsiella spp. and Vibrio spp. were found in few samples. The average load of the pathogens was 104 cfu/g or ml. All the pathogenic isolates were found to be multidrug resistant. Higher resistance was found against penicillin and streptomycin. Tannery waste after lather treatment sample showed antibacterial activity against all the pathogens tested. Overall, presence of pathogenic microorganisms with multidrug resistance traits may pose serious public health threats. Stamford Journal of Microbiology, Vol.7(1) 2017: 14-18

scholarly journals Evaluation of microbial contamination level and the drug susceptible pattern of the isolates cultivated from famous dessert food

Food Research ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 426-430
Author(s):  
T. Akter ◽  
T. Ishma ◽  
S.R. Razzeb ◽  
S.H.M. Uddin ◽  
M. Islam ◽  
...  
Keyword(s):  
Microbial Contamination ◽  
Heterotrophic Bacteria ◽  
Ice Cream ◽  
Drug Susceptibility ◽  
Diffusion Method ◽  
Contamination Level ◽  
Salmonella Spp ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Present study endeavored to evaluate the microbial contamination level along with their drug resistant pattern in some popular desert food items collected from different food shops in Dhaka city, Bangladesh. The microbial evaluation was conducted through conventional cultural methods and drug susceptibility test was executed through disc diffusion method. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 105 CFU/g. In case of specific microflora, the growth of Staphylococcus spp. was very high in sweet, faluda, milk cake and ice cream as estimated up to 105 CFU/g, halua and sweet yogurt showed 104 CFU/g while rest of the samples revealed 103 CFU/g. E. coli was found only in faluda and ice cream up to 103 CFU/g whereas Klebsiella spp. was estimated in all the samples within the range of 102 CFU/g to 105 CFU/g. Salmonella spp., Pseudomonas spp. and Bacillus spp. were totally absent in all the samples. Most of the isolates were found to be resistant against most of the antibiotics. Meanwhile, streptomycin (10 µg), gentamicin (10 µg), azithromycin (15 µg), and nalidixic acid (5 µg) were effective drug against both E. coli and Staphylococcus spp.

Detection and Antibiogram of Different Bacterial Agents From Market Goat Meat

2021 ◽  
Vol 8 (1) ◽  
pp. 135-143
Author(s):  
Md Kamrul Hassan ◽  
Lubna Jahan ◽  
Papia Sultana ◽  
Alamgir Hasan ◽  
Mahbubul Pratik Siddique
Keyword(s):  
Diffusion Method ◽  
Meat Processing ◽  
Salmonella Spp ◽  
Safety Assurance ◽  
Body Regions ◽  
Antibiotic Susceptibility Test ◽  
Spoilage Bacteria ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Sanitary Conditions

The study was conducted to detect various pathogenic as well as spoilage bacteria present in goat meats and also their antibiogram. Different body regions such as brisket, neck and thigh, supplied at different places like Krishi market, Mohakhali kacha bazar, and Charulata market within Dhaka City Corporation area, were taken and the bacteria were isolated. The isolated bacteria were identified on the basis of cultural, morphological, and biochemical characteristics. The isolated bacterial agents were: Staphylococcus spp., Escherichia coli, Bacillus spp., Micrococcus spp., Streptococcus spp., Enterobacter spp., Proteus spp., Citrobacter spp., Salmonella spp., and Pseudomonas spp. Antibiotic susceptibility test using disc diffusion method revealed that most of the isolates were found to be more or less resistant to tetracycline, ampicillin and erythromycin indicating that these may be a threat to consumer’s health. It is emphasized that the present sanitary conditions, as revealed through this study, of meat processing need to be improved so as to minimize microbial contamination. Suggestions are made to introduce Longitudinally Integrated Safety Assurance (LISA) system and Good Manufacturing Practices (GMP) principles. Res. Agric., Livest. Fish.8(1): 135-143, April 2021

scholarly journals Isolation and Identification of Uterine Microorganisms in Postpartum Dairy Cows

2012 ◽  
Vol 28 (1) ◽  
pp. 19-23
Author(s):  
Azizun Nesa ◽  
S D Joy ◽  
M O Faruk
Keyword(s):  
Dairy Cows ◽  
Bacterial Population ◽  
Salmonella Spp ◽  
Isolation And Identification ◽  
Diseased Animal ◽  
Uterine Infection ◽  
Different Types ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Apparently Healthy

The aim of this study was to isolate and identify uterine microorganisms in postpartum cows and to observe  the occurrence of uterine infection in crossbred and local cows. One hundred and nine samples, from both  crossbred and local dairy cows were collected from Chittagong area. They were inseminated artificially  (n=89) and naturally (n=20). Samples were collected at least 3 months after calving. Among the selected cows,  33 had different reproductive diseases and the rest were from apparently healthy cows. The crossbred dairy  cows were more susceptible to reproductive diseases (19.27%) than the local ones. Uterine infection was  higher (52%) in cows inseminated artificially than that of natural ones (21.21%). Nineteen different types of  bacteria were isolated from both diseased and healthy animals, except Clostridium spp and Nocardia spp which  were not found in diseased animal. Likewise, Bacillus spp and Salmonella spp were absent in healthy animals.  Most prevalent bacteria in diseased animal were Staphylococcus spp and Streptococcus spp found in 33% and  27.3% of the cases respectively. In non-diseased cases, the highest number of samples was positive for  Bacteroied spp (13.2%) followed by Streptococcus spp (10.4%). On average, the highest number of bacterial  population was isolated from diseased samples and most of those bacteria are pathogenic. DOI: http://dx.doi.org/10.3329/bjm.v28i1.11804 Bangladesh J Microbiol, Volume 28, Number 1, June 2011, pp 19-23

scholarly journals Microbiological quality of honey from stingless bee, jandaíra (Melipona subnitida), from the semiarid region of Brazil

2018 ◽  
Vol 48 (9) ◽  
Author(s):  
Carolina de Gouveia Mendes da Escóssia Pinheiro ◽  
Maria Rociene Abrantes ◽  
Rodrigo Otávio Silveira Silva ◽  
Carlos Augusto Oliveira Junior ◽  
Francisco Carlos Faria Lobato ◽  
...  
Keyword(s):  
Clostridium Perfringens ◽  
Microbiological Quality ◽  
Stingless Bee ◽  
Semiarid Region ◽  
Salmonella Spp ◽  
Mesophilic Bacteria ◽  
Melipona Subnitida ◽  
Bacillus Spp ◽  
Staphylococcus Spp

ABSTRACT: The aim of this study was to evaluate the presence of microorganisms in honey produced by the stingless bee (SB) jandaíra (Melipona subnitida) from the semiarid region of Brazil. Thirty-five samples were analyzed and all of them were positive for mesophilic bacteria, coliforms at 45°C, fungi, and yeast. Staphylococcus spp. were identified in 85.7%, while Bacillus spp. were detected in 34.3% of honey samples. DNAs of Clostridium perfringens and C. botulinum were detected in 40% and 2.8% of the samples, respectively. Salmonella spp. and C. difficile were not detected. The present research revealed a great diversity of microorganisms in honey produced by jandaíra.

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