Cytotoxicity and antioxidant activity of goldenberry extracts obtained with high intensity ultrasound

Cristiane Franco Codevilla; Bruna Tischer; Amanda Leitão Gindri; Daniele Rubert Nogueira-Librelotto; Juliano Smanioto Barin; Cristiane de Bona da Silva; Clarice Madalena Bueno Rolim; Leila Queiroz Zepka; Cristiano Ragagnin de Menezes

doi:10.1590/0103-8478cr20170362

Cytotoxicity and antioxidant activity of goldenberry extracts obtained with high intensity ultrasound

Ciência Rural ◽

10.1590/0103-8478cr20170362 ◽

2018 ◽

Vol 48 (2) ◽

Cited By ~ 5

Author(s):

Cristiane Franco Codevilla ◽

Bruna Tischer ◽

Amanda Leitão Gindri ◽

Daniele Rubert Nogueira-Librelotto ◽

Juliano Smanioto Barin ◽

...

Keyword(s):

Phenolic Compounds ◽

High Intensity ◽

In Vitro Cytotoxicity ◽

Antioxidant Compounds ◽

High Intensity Ultrasound ◽

Ultrasound Assisted Extraction ◽

Wide Range ◽

Conventional Extraction ◽

Time Required

ABSTRACT: The high intensity ultrasound-assisted extraction (HIU) is one of the most simple, quick and efficient techniques for the extraction of phenolic and other antioxidant compounds from plants. This is the first application of HIU for the extraction of these compounds from goldenberry fruit. The HIU and conventional extraction techniques showed similar results regarding to phenolic compounds and antioxidant capacity. However, the time required for HIU extraction (5min) was 24 times lower than conventional extraction (120min). Phenolic compounds reported were chlorogenic acid, caffeic acid and rutin. In vitro cytotoxicity assays were used for evaluation of extracts and the results showed that in a wide range of concentration, the extract maintains cell viability, thus indicating the possibility to use it as food with safety.

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High-intensity ultrasound-assisted extraction of phenolic compounds from cowpea pods and its encapsulation in hydrogels

Heliyon ◽

10.1016/j.heliyon.2020.e04410 ◽

2020 ◽

Vol 6 (7) ◽

pp. e04410

Author(s):

Maria Victoria Traffano-Schiffo ◽

Tatiana Rocio Aguirre Calvo ◽

María Victoria Avanza ◽

Patricio R. Santagapita

Keyword(s):

Phenolic Compounds ◽

High Intensity ◽

High Intensity Ultrasound ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

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Characterization, Stability, and Bioaccessibility of Betalain and Phenolic Compounds from Opuntia stricta var. Dillenii Fruits and Products of Their Industrialization

Foods ◽

10.3390/foods10071593 ◽

2021 ◽

Vol 10 (7) ◽

pp. 1593

Author(s):

Iván Gómez-López ◽

Gloria Lobo-Rodrigo ◽

María P. Portillo ◽

M. Pilar Cano

Keyword(s):

Phenolic Compounds ◽

Phenolic Acids ◽

Total Phenolic ◽

Antioxidant Compounds ◽

Gastrointestinal Digestion ◽

Fruit Peel ◽

Full Characterization ◽

In Vitro Gastrointestinal Digestion ◽

Quercetin Glycoside

The aim of the present study was the full characterization, quantification, and determination of the digestive stability and bioaccessibility of individual betalain and phenolic compounds of Opuntia stricta, var. Dillenii fresh fruits (peel, pulp, and whole fruit) and of the products of the industrialization to obtain jam (raw pressed juice (product used for jam formulation), by-product (bagasse), and frozen whole fruit (starting material for jam production)). Opuntia stricta var. Dillenii fruits and products profile showed 60 betalain and phenolic compounds that were identified and quantified by HPLC-DAD-ESI/MS and HPLC-DAD-MS/QTOF, being 25 phenolic acids (including isomers and derivatives), 12 flavonoids (including glycosides), 3 ellagic acids (including glycosides and derivative), and 20 betanins (including degradation compounds). In vitro gastrointestinal digestion was performed by INFOGEST® protocol. Fruit pulp showed the greater content of total betalains (444.77 mg/100 g f.w.), and jam only showed very low amounts of two betanin degradation compounds, Cyclo-dopa-5-O-β-glucoside (and its isomer) (0.63 mg/100 f.w.), and two Phyllocactin derivatives (1.04 mg/100 g f.w.). Meanwhile, fruit peel was the richer tissue in total phenolic acids (273.42 mg/100 g f.w.), mainly in piscidic acid content and total flavonoids (7.39 mg/100 g f.w.), isorhamnetin glucoxyl-rhamnosyl-pentoside (IG2) being the most abundant of these compounds. The stability of betalains and phenolic compounds during in vitro gastrointestinal digestion is reported in the present study. In Opuntia stricta var. Dillenii pulp (the edible fraction of the fresh fruit), the betanin bioaccessibility was only 22.9%, and the flavonoid bioaccessibility ranged from 53.7% to 30.6%, depending on the compound. In non-edible samples, such as peel sample (PE), the betanin bioaccessibility was 42.5% and the greater bioaccessibility in flavonoids was observed for quercetin glycoside (QG1) 53.7%, the fruit peel being the most interesting material to obtain antioxidant extracts, attending to its composition on antioxidant compounds and their bioaccessibilities.

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Effects of high-intensity ultrasound process parameters on the phenolic compounds recovery from araticum peel

Ultrasonics Sonochemistry ◽

10.1016/j.ultsonch.2018.09.002 ◽

2019 ◽

Vol 50 ◽

pp. 82-95 ◽

Cited By ~ 17

Author(s):

Henrique S. Arruda ◽

Eric Keven Silva ◽

Gustavo A. Pereira ◽

Célio Fernando F. Angolini ◽

Marcos N. Eberlin ◽

...

Keyword(s):

Phenolic Compounds ◽

Process Parameters ◽

High Intensity ◽

High Intensity Ultrasound

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Enhanced Recovery of Antioxidant Compounds from Hazelnut (Corylus avellana L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities

Antioxidants ◽

10.3390/antiox8100460 ◽

2019 ◽

Vol 8 (10) ◽

pp. 460 ◽

Cited By ~ 8

Author(s):

Rusu ◽

Fizeșan ◽

Pop ◽

Gheldiu ◽

Mocan ◽

...

Keyword(s):

Bioactive Compounds ◽

Enhanced Recovery ◽

Biological Activities ◽

Natural Antioxidants ◽

Antioxidant Compounds ◽

Cancerous Cell ◽

Wide Range ◽

Quantitative Analyses ◽

Corylus Avellana L

Tree nut by-products could contain a wide range of phytochemicals, natural antioxidants, which might be used as a natural source for dietary supplements. The aim of the present study was to evaluate the phenolic and sterolic composition, as well as the antioxidant and other biological activities, of hazelnut involucre (HI) extracts. Experimental designs were developed in order to select the optimum extraction conditions (solvent, temperature, time) using turbo-extraction by Ultra-Turrax for obtaining extracts rich in bioactive compounds. Qualitative and quantitative analyses were performed by LC-MS and LC-MS/MS and they revealed important amounts of individual polyphenols and phytosterols, molecules with antioxidant potential. The richest polyphenolic HI extract with the highest antioxidant activity by TEAC assay was further evaluated by other in vitro antioxidant tests (DPPH, FRAP) and enzyme inhibitory assays. Additionally, the cytotoxic and antioxidant effects of this extract on two cancerous cell lines and on normal cells were tested. This is the first study to analyze the composition of both hydrophilic and lipophilic bioactive compounds in HI extracts. Our findings reveal that this plant by-product presents strong biological activities, justifying further research, and it could be considered an inexpensive source of natural antioxidants for food, pharmaceutical, or cosmetic industry.

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IN VITRO CYTOTOXICITY AND ANTIOXIDANT EVALUATION OF BIOGENIC SYNTHESIZED GOLD NANOPARTICLES FROM MARSILEA QUADRIFOLIA ON LUNG AND OVARIAN CANCER CELLS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2018v10i5.27999 ◽

2018 ◽

Vol 10 (5) ◽

pp. 153 ◽

Cited By ~ 1

Author(s):

Balashanmugam P. ◽

Mosa Christas K. ◽

Kowsalya E.

Keyword(s):

Gold Nanoparticles ◽

Cell Lines ◽

Aqueous Extract ◽

A549 Cell ◽

In Vitro Cytotoxicity ◽

Spherical Particles ◽

X Ray ◽

Marsilea Quadrifolia ◽

Wide Range

Objective: The biogenic gold nanoparticles are considered to be extremely impressive for its wide range of applications in pharmaceutics and therapeutics. The present study was aimed at the biogenic synthesis of gold nanoparticles (AuNPs) from Marsilea quadrifolia aqueous extract and to investigate its antioxidant property and cytotoxic effect on human ovarian teratocarcinoma (PA-1) and lung adenocarcinoma (A549) cell lines.Methods: The biogenic AuNPs was synthesized using an aqueous extract of Marsilea quadrifolia. The synthesized biogenic AuNPs were characterized by ultraviolet (UV) visible spectroscopy, transmission electron microscopy (TEM), energy dispersive X-ray analysis (EDX) and X-ray diffraction (XRD). The biogenic AuNPs was assessed for its stability over a period of time and antioxidant activity. The cytotoxicity of biogenic AuNPs against PA-1 and A549 cell lines was studied using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay.Results: The synthesized biogenic AuNPs showed peculiar ruby red color and a surface plasmon resonance (SPR) peak at 544 nm in the UV-Vis spectrum. The characterization of biogenic AuNPs by TEM, EDX and XRD revealed well dispersed spherical particles ranging from 10-40 nm and the presence of elemental gold and its crystalline nature, respectively. The AuNPs showed good stability and the scavenging activity at 50 μg/ml. The in vitro cytotoxicity of biogenic AuNPs against PA-1 and A549 cell lines recorded half maximal inhibitory concentration (IC50) of 45.88 μg/ml and 52.015 μg/ml, respectively.Conclusion: The biogenic AuNPs demonstrated superior antioxidant and antiproliferative activities against cancer cell lines.

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Passive elastography monitoring of a high intensity ultrasound treatment: A study of feasibility in in vitro liver

The Journal of the Acoustical Society of America ◽

10.1121/1.5068430 ◽

2018 ◽

Vol 144 (3) ◽

pp. 1925-1925

Author(s):

Bruno Giammarinaro ◽

Victor Barre`re ◽

David Melodelima ◽

Stefan Catheline

Keyword(s):

High Intensity ◽

Ultrasound Treatment ◽

High Intensity Ultrasound

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Pleiotropic Biological Effects of Dietary Phenolic Compounds and their Metabolites on Energy Metabolism, Inflammation and Aging

Molecules ◽

10.3390/molecules25030596 ◽

2020 ◽

Vol 25 (3) ◽

pp. 596 ◽

Cited By ~ 3

Author(s):

María del Carmen Villegas-Aguilar ◽

Álvaro Fernández-Ochoa ◽

María de la Luz Cádiz-Gurrea ◽

Sandra Pimentel-Moral ◽

Jesús Lozano-Sánchez ◽

...

Keyword(s):

Energy Metabolism ◽

Phenolic Compounds ◽

Molecular Mechanisms ◽

Biological Effects ◽

Biological Properties ◽

In Vivo Studies ◽

Wide Range ◽

High Prevalence

Dietary phenolic compounds are considered as bioactive compounds that have effects in different chronic disorders related to oxidative stress, inflammation process, or aging. These compounds, coming from a wide range of natural sources, have shown a pleiotropic behavior on key proteins that act as regulators. In this sense, this review aims to compile information on the effect exerted by the phenolic compounds and their metabolites on the main metabolic pathways involved in energy metabolism, inflammatory response, aging and their relationship with the biological properties reported in high prevalence chronic diseases. Numerous in vitro and in vivo studies have demonstrated their pleiotropic molecular mechanisms of action and these findings raise the possibility that phenolic compounds have a wide variety of roles in different targets.

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High-intensity ultrasound-assisted extraction of Garcinia madruno biflavonoids: Mechanism, kinetics, and productivity

Biochemical Engineering Journal ◽

10.1016/j.bej.2020.107676 ◽

2020 ◽

Vol 161 ◽

pp. 107676

Author(s):

Luis Carrillo-Hormaza ◽

Luisa Duque ◽

Sebastián López-Parra ◽

Edison Osorio

Keyword(s):

High Intensity ◽

High Intensity Ultrasound ◽

Ultrasound Assisted Extraction ◽

Assisted Extraction ◽

Ultrasound Assisted

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Phenol Profiling and Nutraceutical Potential of Lycium spp. Leaf Extracts Obtained with Ultrasound and Microwave Assisted Techniques

Antioxidants ◽

10.3390/antiox8080260 ◽

2019 ◽

Vol 8 (8) ◽

pp. 260 ◽

Cited By ~ 9

Author(s):

Luna Pollini ◽

Rachele Rocchi ◽

Lina Cossignani ◽

Jordi Mañes ◽

Dario Compagnone ◽

...

Keyword(s):

Antioxidant Properties ◽

In Vitro Assays ◽

Array Detector ◽

Antioxidant Compounds ◽

Ultrasound Assisted Extraction ◽

Leaf Extracts ◽

Phenol Compounds ◽

Microwave Assisted ◽

Assisted Extraction

In recent years, agricultural and industrial residues have attracted a lot of interest in the recovery of phytochemicals used in the food, pharmaceutical, and cosmetic industries. In this paper, a study on the recovery of phenol compounds from Lycium spp. leaves is presented. Ultrasound-assisted extraction (UAE) and microwave-assisted extraction (MAE) have been used with alcoholic and hydroalcoholic solvents. Methanolic UAE was the most successful technique for extracting phenols from Lycium leaves, and we used on leaves from L. barbarum and L. chinense cultivated in Italy. The extracts were then characterized as regards to the antioxidant properties by in vitro assays and the phenol profiling by a high performance liquid chromatography-diode array detector (HPLC-DAD). Chlorogenic acid and rutin were the main phenol compounds, but considerable differences have been observed between the samples of the two Lycium species. For example, cryptochlorogenic acid was found only in L. barbarum samples, while quercetin-3-O-rutinoside-7-O-glucoside and quercetin-3-O-sophoroside-7-O-rhamnoside only in L. chinense leaves. Finally, multivariate statistical analysis techniques applied to the phenol content allowed us to differentiate samples from different Lycium spp. The results of this study confirm that the extraction is a crucial step in the analytical procedure and show that Lycium leaves represent an interesting source of antioxidant compounds, with potential use in the nutraceutical field.

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