scholarly journals Risk factors associated with intramammary colonization with Mollicutes in dairy cattle from Southeast Brazil

2021 ◽  
Vol 51 (8) ◽  
Author(s):  
Ana Carolina Nunes de Morais ◽  
Danielle Regis Pires ◽  
Nathalie Costa da Cunha ◽  
Leandro dos Santos Machado ◽  
Michel Abdalla Helayel ◽  
...  
Keyword(s):  
Risk Factors ◽  
Rio De Janeiro ◽  
Dairy Herds ◽  
Chain Reaction ◽  
Chi Square ◽  
Intramammary Infection ◽  
Factors Associated ◽  
Southeast Brazil ◽  
Chi Square Test ◽  
Polymerase Chain

ABSTRACT: Bacteria of Mollicutes Class are associated with intramammary infection and decrease in milk production. This study investigated the occurrence of Mollicutes and elucidated their risk factors in dairy herds from Southeast Brazil. For this, milk samples from 387 lactation cows from Minas Gerais, Rio de Janeiro and São Paulo States were subjected to the polymerase chain reaction (PCR) to detect Mollicutes. Species of Mycoplasma were investigated in Mollicutes positive samples by PCR, including Mycoplasma bovis, M. alkalescens, M. bovigenitalium, M. bovirhinis, M. arginini and A. laidlawii. An epidemiological questionnaire was applied to collect data on possible risk factors, which were assessed using Pearson’s Chi-square test followed by odds ratio (P≤0.05). Mollicutes were reported in 21% (4/19) of the herds and 4% (16/387) of the animals, while 1% (5/387) were positive for M. bovis and 3% (11/387) for M. arginini. All samples were negative to the other agents. Herds with more than 150 animals [OR=3.51 (95% CI 1.11-11.08)], manual milking [OR=9.97 (95% CI 2.80-35.49)] and not-milking animals with mastitis last [OR=6.54 (95% CI 1.92-22.29)] were risk factors. The presence of these conditions may favor intramammary infection by Mollicutes in dairy herds from Southeast Brazil. This is the first report of M. bovis in Rio de Janeiro and M. arginini in the studied states.

scholarly journals Universally Primed Polymerase Chain Reaction Alleles and Internal Transcribed Spacer Restriction Fragment Length Polymorphisms Distinguish Two Subgroups in Botrytis aclada Distinct from B. byssoidea

2001 ◽  
Vol 91 (6) ◽  
pp. 527-533 ◽  
Author(s):  
K. Nielsen ◽  
A. F. Justesen ◽  
D. Funck Jensen ◽  
D. S. Yohalem
Keyword(s):  
Polymerase Chain Reaction ◽  
Internal Transcribed Spacer ◽  
Restriction Analysis ◽  
Group Method ◽  
Chain Reaction ◽  
Chi Square ◽  
Bootstrap Analysis ◽  
Pair Group ◽  
Chi Square Test ◽  
Polymerase Chain

Fifty-one isolates representing the four Botrytis spp. associated with onion neck rot were clustered by unweighted pair group method with arithmetic mean based on universal-primed polymerase chain reaction (UP-PCR) fingerprints. Bootstrap analysis of the consensus phenogram clearly demonstrated five strong clusters among the four Botrytis spp.: B. cinerea (C), B. squamosa (S), B. byssoidea (B), and B. aclada (AI and AII). Subdivision of the 30 B. aclada isolates, AI (14) and AII (16), from Europe, Egypt, North America, and Japan was further supported by restriction analysis of the internal transcribed spacer of the ribosomal genes and spore size measurements. Gene diversities (H) among AI and AII isolates were very low (0.007 and 0.043, respectively). A likelihood ratio chi-square test (G2) of Nei's coefficient of genetic differentiation (GST) showed that both B. aclada subgroups, AI and AII, were significantly different from B. byssoidea (P < 0.001), and that B. aclada subgroups AI and AII were significantly different from each other (P < 0.001). No UP-PCR alleles were shared by AI and B. byssoidea isolates, whereas 10 and 12 alleles were shared by AI:AII and AII:B. byssoidea, respectively. The hypothesis that AII may be a hybrid between AI and B. byssoidea is discussed.

scholarly journals Surgical Site Infections (SSI) and factors associated in a private tertiary care teaching hospital in Raichur, Karnataka, India

2018 ◽  
Vol 5 (8) ◽  
pp. 2899
Author(s):  
Prashanta Swami Pujar ◽  
K. B. Phuleker ◽  
Nagaraj Bhalki
Keyword(s):  
Risk Factors ◽  
Tertiary Care ◽  
Surgical Site Infections ◽  
Research Centre ◽  
Chi Square ◽  
College Hospital ◽  
Factors Associated ◽  
Duration Of Surgery ◽  
Chi Square Test ◽  
A Prospective Study

Background: Prevention of Surgical site infection (SSI) remains a focus of attention because wound infections continue to be a major source of expense, morbidity, and even mortality. Three quarters of deaths of surgical patients with SSIs are attributed to nosocomial infections, nearly all of which are organ/space infections. The objective of the present study was to estimate the incidence of SSI and to study the various risk factors associated with SSI.Methods: This is a prospective study of 180 eligible cases eligible subjects, who underwent various surgeries in the department of General Surgery after applying inclusion and exclusion criteria. The study conducted at the Navodaya Medical College Hospital and Research Centre, Raichur. The tenure for the study was April 2017 to June 2017. Data was collected using pretested proforma. Data was analysed using SPSS version 16. Chi -square test and multiple logistic regression was applied to know the association between various risk factors and occurrence of SSI.Results: Among 180 patients 33 (18.33%) developed surgical site infections (SSI). Among 33 SSIs 25 (75.76%) were grade 3 and 8 (24.24%) were grade 4 infections. SSIs were found more commonly among patients over 50 years, diabetics, HIV infected patients, patients with longer duration of surgery and associations with these factors were found statistically significant.Conclusions: The incidence rate of SSI was quite high, and its end results will have a greater impact on patients as well as on healthcare systems. Prevention of SSI requires multipronged approach targeting both patient related and procedure related risk factors in pre- operative, intra-operative, and post-operative period.

scholarly journals Risk factors associated with the occurrence of Cryptosporidium parvum infection in calves

2010 ◽  
Vol 62 (6) ◽  
pp. 1325-1330 ◽  
Author(s):  
A.J Almeida ◽  
F.C.R Oliveira ◽  
V.M.Q Flores ◽  
C.W.G Lopes
Keyword(s):  
Risk Factors ◽  
Cryptosporidium Parvum ◽  
Dairy Calves ◽  
Chain Reaction ◽  
Factors Associated ◽  
Water Trough ◽  
Polymerase Chain ◽  
Cryptosporidium Parvum Oocysts ◽  
Statistical Risk ◽  
Janeiro State

Cryptosporidium parvum oocysts were detected in feces of dairy calves raised in Rio de Janeiro State and the risk factors involved in the infection were determined. A hundred calves aging up to 12-month-old from 13 dairy farms were sampled. Polymerase chain reaction was used to detect the presence of oocysts. The zoonotic C. parvum species was detected in 45% animals. Statistical risk factors analyses revealed an association between infection and animals raised in technical systems such as the use of milking equipment, milking cooler, and water trough(P<0.05)

scholarly journals Headache associated with COVID-19: Frequency, characteristics and association with anosmia and ageusia

Cephalalgia ◽  
2020 ◽  
Vol 40 (13) ◽  
pp. 1443-1451 ◽  
Author(s):  
Pedro Augusto Sampaio Rocha-Filho ◽  
João Eudes Magalhães
Keyword(s):  
Polymerase Chain Reaction ◽  
Cross Sectional Study ◽  
Sectional Study ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Chi Square ◽  
Cross Sectional ◽  
Severe Intensity ◽  
Chi Square Test ◽  
Polymerase Chain

Objectives To assess the frequency and characteristics of headache in patients with COVID-19 and whether there is an association between headache and anosmia and ageusia. Methods This was a cross-sectional study. Consecutive patients admitted to hospital with COVID-19, confirmed by reverse transcription polymerase chain reaction (RT-PCR) technique, were assessed by neurologists. Results Seventy-three patients were included in the study, 63% were male; the median age was 58 years (IQR: 47–66). Forty-seven patients (64.4%) reported headaches, which had most frequently begun on the first day of symptoms, were bilateral (94%), presenting severe intensity (53%) and a migraine phenotype (51%). Twelve patients (16.4%) presented with headache triggered by coughing. Eleven (15%) patients reported a continuous headache. Twenty-eight patients (38.4%) presented with anosmia and 29 (39.7%) with ageusia. Patients who reported hyposmia/anosmia and/or hypogeusia/ageusia experienced headache more frequently than those without these symptoms (OR: 5.39; 95% CI:1.66–17.45; logistic regression). Patients with anosmia and ageusia presented headache associated with phonophobia more often compared to those with headache without these complaints (Chi-square test; p < 0.05). Headache associated with COVID-19 presented a migraine phenotype more frequently in those experiencing previous migraine ( p < 0.05). Conclusion Headaches associated with COVID-19 are frequent, are generally severe, diffuse, present a migraine phenotype and are associated with anosmia and ageusia.

scholarly journals Serum MALAT1 Assumes Signifying Capacity in Gastric Cancer Diagnosis.

2020 ◽  
Author(s):  
Keqian Zhang ◽  
Tianqi Mao ◽  
Zhicheng He ◽  
Xiaojiao Wu ◽  
Yu Peng ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Roc Analysis ◽  
Operating Characteristic ◽  
Area Under The Curve ◽  
Diagnostic Value ◽  
Chain Reaction ◽  
Serum Samples ◽  
Chi Square ◽  
Chi Square Test ◽  
Polymerase Chain

Abstract Background: Gastric cancer (GC) represents one of the most serious cancers worldwide with the increasing mortality. Metastasis associated lung adenocarcinoma transcript 1 (MALAT1), a kind of lncRNAs, has been reported to be involved in the progression of cancers. This study aimed to assess serum expression pattern of MALAT1 and its clinical significance in diagnosis of GC.Methods: Serum specimens were collected from 120 GC patients and 58 healthy individuals. The expression profile of MALAT1 was examined using quantitative real-time polymerase chain reaction (qRT-PCR), and its association with clinical parameters was estimated by chi-square test. The diagnostic value of MALAT1 in GC was evaluated by the receiver operating characteristic (ROC) analysis.Results: Upregulated expression of MALTA1 was found in GC patients compared with the healthy controls (P<0.05). The overexpression of MALAT1 was positively correlated with lymph node metastasis (P=0.041) and TNM stage (P=0.005). An area under the curve (AUC) was 0.897 in ROC analysis, suggesting the high diagnostic value of MALAT1. Conclusion: The expression of MALAT1 was upregulated in GC serum samples, and its expression might serve as a potential diagnostic biomarker in patients with GC.

scholarly journals Prevalence and risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa

2017 ◽  
Vol 4 (2) ◽  
Author(s):  
Carlos Víctor Hernández Ramírez ◽  
Soila Maribel Gaxiola Camacho ◽  
Ignacio Osuna Ramirez ◽  
Idalia Enríquez Verdugo ◽  
Nohemí Castro del Campo ◽  
...  
Keyword(s):  
Risk Factors ◽  
Statistical Significance ◽  
Epidemiological Survey ◽  
Serum Samples ◽  
Chi Square ◽  
Factors Associated ◽  
Epidemiological Risk ◽  
Chi Square Test ◽  
Sanitary Conditions ◽  
The City

Veterinaria México OA ISSN: 2448-6760Cite this as:Hernández Ramírez CV, Gaxiola Camacho SM, Osuna Ramirez I, Enríquez Verdugo I, Castro del Campo N, López Moreno HS. Prevalence and risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa. Veterinaria México OA 2017;4(2).doi: 10.21753/vmoa.2.2.369Domestic dogs transmit Leptospira spp. to humans, and determining the health risk that they represent is of paramount importance. To determine the seroprevalence and main risk factors associated with serovars of Leptospira in dogs from Culiacan, Sinaloa, we obtained serum samples from 165 dogs. The samples were stored at -40 °C and were analysed by the microbiology laboratory at Centro Nacional de Sanidad Animal using the leptospirosis microscopic agglutination test. Additionally, a survey was performed to identify epidemiological risk factors, and statistical inference was determined using chi-square test, odd ratios, and logistic regression with a statistical significance set at P < 0.05. The prevalence of Leptospira was 9 % (15/165), and we identified seven serovars: canicola 17 (46 %), icterohaemorrhagiae (40 %), bratislava (40 %), grippotyphosa (33 %), shermani (33 %), pyrogenes (20 %) and ballum (13 %). Based on our epidemiological survey, the risk factors associated with the detection of antibodies against Leptospira include the permanent habitation of pets in courtyards (OR = 4.6, P < 0.05) and presence of water stored in drums and basins (OR = 3.25, P < 0.05). The prevalence of leptospirosis in dogs indicates that the disease is present in the city of Culiacan and that leptospiral antibodies in dogs increase in poor sanitary conditions with stored water, which increases the potential risk of infection for both humans and animals.Figure 1. Frequency of observed serovars of Leptospira

scholarly journals Clinical Characteristics And Analysis Of Risk Factors For Disease Progression Of COVID-19 Among Patients Within Wad Medani Isolation Centers, Gezira State: A Prospective Cohort Study From Sudan

2021 ◽  
Author(s):  
Mohammed Yousif Elnaeem Yousif ◽  
Moh.Mah.Fadel Allah Eljack ◽  
Osman Amir ◽  
Mohammed Alfatih ◽  
Akram Khalid Al Tigany Al Shiekh ◽  
...  
Keyword(s):  
Risk Factors ◽  
Cohort Study ◽  
Mortality Rate ◽  
Clinical Characteristics ◽  
Severe Disease ◽  
Severe Infection ◽  
Chi Square ◽  
Factors Associated ◽  
Glucose Levels ◽  
Chi Square Test

Abstract Background : (COVID-19) had a great impact on the world’s health systems since December 2019. A little is known about the clinical characteristics and risk factors associated with COVID-19 severity in Sudanese Patients; therefor it is necessary to summarize the clinical characteristics of patients with COVID-19 and to explore the risk factors associated with COVID-19 severity. Methods : A one-year retrospective cohort study (May 2020- May2021) was done at three isolation centers in Wad Medani. Sample contained all COVID-19 patients who are over 18 years old and were confirmed to be COVID-19 by nucleic acid testing or features Suggestive of Covid19 on Chest CT scan. Results : This study included 418 patients confirmed COVID-19 cases with a median age of 66.3±13years. 179 (64.2%) patients were men. Hypertension (n=195; 46.7%) and diabetes (n=187; 44.7%) were the most common comorbidities. The most common symptoms at COVID-19 onset were fever (n=303; 72.5%), cough (n=278; 66.5%) and dyspnea (n= 256; 61.2%). the overall mortality rate was 35.4% (n=148). The morality rate was 42.3% (n=118) among patients with severe disease. The Chi-square test and ANOVA analysis revealed that older age, anemia, neutrophilia and lymphcytopenia, higher glucose levels, HbA1c levels and creatinine levels were variables associated with severe COVID-19. In inflammatory markers, the levels of CRP and d-dimer were elevated in severe infection more than moderate and mild infections. Conclusion : Patients with these factors are more likely to deteriorate into severe infection and have higher mortality rate than those without these factors.

scholarly journals Risk factors associated with swine dysentery in East-European pig production units

2005 ◽  
Vol 59 (3-4) ◽  
pp. 349-362
Author(s):  
C.P. Mirko ◽  
G. Bilkei
Keyword(s):  
Risk Factors ◽  
Strong Association ◽  
Fiber Content ◽  
Chain Reaction ◽  
Swine Dysentery ◽  
Brachyspira Hyodysenteriae ◽  
East European ◽  
Factors Associated ◽  
Polymerase Chain ◽  
Influential Variable

The objective of the present study was to determine the risk factors for swine dysentery in East-European middle-size to large farrow to finish units with separate breeding and grower-finisher facilities. Ten breeding animals (3-10% of the female inventory) and 10 grower tinisher pigs (80-140 days-of age) were sampled in each herd for polymerase chain reaction testing (PCR) for Brachyspira hyodysenteriae (B hyo) in their feces. Of 139 farrow to finish units, 51 (36.7%) were positive, 49 (35,3%) were negative, and 39 (28.1%) were inconclusive for B hyo by PCR. In breeding subunits, twelve variables passed the screening criterion for risk factors (P<.2) for B hyo PCR positivity. The odds of the breeding subunits being B hyo PCR positive were 3.5 times greater when the grower-finisher subunit was positive and the fiber content of the diet was >6%. Use of "all in all out" farrowing policy and having >60% multiparous sows, each reduced the odds of being B hyo PCR positive about fourfold. In growing-finishing subunits, fourteen variables passed the screening criterion for risk factors (P<-2) for B hyo PCR positivity. B hyo PCR positive status of the breeding subunits and higher fiber content of the diet were the most influential variable, with the odds of the grower-finisher subunits being B hyo PCR positive almost eight times greater when the breeding subunit was also B hyo PCR positive. Grower-finisher B hyo PCR positivity was also associated with the percentage of pigs housed on concrete slats, with the odds of being positive 7.5 times higher for subunits where more that 70% of the animals were kept on concrete slats compared to all other floor types. There was a strong association between grower-finisher status and whether the animals were in outdoor lots with the odds of being B hyo PCR positive substantially lower for pigs in outdoor lots compared with all other surfaces.

scholarly journals FAKTOR-FAKTOR RISIKO YANG BERHUBUNGAN DENGAN KEJADIAN PLASENTA PREVIA PADA IBU BERSALIN DI RSUD DAYA KOTA MAKASSAR

2021 ◽  
Vol 5 (1) ◽  
Author(s):  
Yudiarsi Eppang
Keyword(s):  
Risk Factors ◽  
Pregnant Women ◽  
Quantitative Research ◽  
Health Workers ◽  
Placenta Previa ◽  
Chi Square ◽  
Cross Sectional ◽  
Factors Associated ◽  
Chi Square Test ◽  
History Of

The aim of the study: To determine the risk factors associated with the incidence of placenta previa in mothers who gave birth at RSUD Daya Makassar City. Method: Quantitative Research, with a cross sectional approach. The design of this study was used with the goal to knowing the risk factors associated with the incidence of placenta previa in mothers who gave birth at RSUD Daya Kota Makassar in 2020. The population in this study were all mothers who experienced placenta previa recorded as data in the INC room at RSUD. Daya City of Makassar in 2020. The sample in this study were women who experienced placenta previa recorded in the INC room data at RSUD Daya Kota Makassar in 2020. Results: This study shows that 15 respondents studied showed that there was a relationship between age, parity, history of curettage, and the distance between pregnancies to the incidence of placenta previa. Based on the results of the chi square test, the results obtained were age (p = 0.019), parity (p = 0.005), history of curettage (p = 0.020) and pregnancy distance (p = 0.020). Conclusion: in this study there is a significant relationship between age, parity, history of curettage, and the distance between pregnancies to the incidence of placenta previa at RSUD Daya Kota Makassar. Suggestion: Suggestions for health workers need to increase antenatal supervision of pregnant women who have a history of obstetrics, so that risky pregnant women can be managed properly

Prevalence and risk factors associated with endoparasitosis of dogs and cats in Espírito Santo, Brazil

2016 ◽  
Vol 61 (3) ◽  
Author(s):  
Diefrey Ribeiro Campos ◽  
Luanna Castro Oliveira ◽  
Daniele Fassina de Siqueira ◽  
Lívia Reisen Perin ◽  
Nayara Camatta Campos ◽  
...  
Keyword(s):  
Risk Factors ◽  
Confidence Intervals ◽  
Odds Ratios ◽  
Chi Square ◽  
Espírito Santo ◽  
Factors Associated ◽  
Faecal Samples ◽  
Chi Square Test ◽  
Espirito Santo

AbstractThe objective of this study was to determine the prevalence and risk factors for the occurrence of endoparasitosis in dogs and cats in Espírito Santo, Brazil. For the study, 345 dogs and 160 cats were examined. Faecal samples from the animals were collected, and owners were interviewed about their handling of their animals. For the diagnosis of the infections, the Willis-Molley, simple centrifugal flotation and formalin-ether sedimentation techniques were performed. The data found in the tests were tabulated and analysed with a chi-square test (p <0.05), and calculation of odds ratios (OR) with confidence intervals of 95% were then performed to determine the association between the variables and the outcome of stool examinations. The prevalence of parasites was 59% for dogs and 54% for cats. The genus

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