scholarly journals Total antioxidant activity of yacon tubers cultivated in Brazil

2016 ◽  
Vol 40 (5) ◽  
pp. 596-605 ◽  
Author(s):  
Juciane Abreu Ribeiro Pereira ◽  
Meryene Carvalho Teixeira ◽  
Adelir Aparecida Saczk ◽  
Maria de Fátima Piccolo Barcelos ◽  
Marcelo Firmino de Oliveira ◽  
...  

ABSTRACT Yacon (Smallanthus sonchifolius) is a tuberous root from the Andean region in the South America rich em water, fructooligosaccharides and phenolic compounds, some of which are natural antioxidants and may help prevent the deleterious action of free radicals in the body. The yacon has attracted much attention due to their potential health benefits to humans. In this study the levels of total phenolics, tannins, phenolic acids, and total antioxidant activity were measured in the peel and pulp of yacon tubers both in the fresh and flour forms. The flours of yacon presented higher concentrations of total phenolics and tannins, especially peel flour. The yacon pulp flour stood out as the main source of phenolic acids, mainly caffeic and chlorogenic acid. The total antioxidant activity assessed by DPPH (2,2-Diphenyl-1-picrylhydrazyl) and ABTS (2,2′-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid)) assays was higher in the yacon peel flour. The total antioxidant activity was correlated with the total phenolic content and tannins by the DPPH and ABTS assays. These results suggest that yacon can be used as an alternative food source of phenolic compounds that help prevent degenerative processes caused by oxidative stress, especially in the flours form.

2018 ◽  
Vol 48 (3) ◽  
pp. 386-396 ◽  
Author(s):  
Vania Silva Carvalho ◽  
Ana Carolina Conti-Silva

Purpose This paper aims to examine the bioactive compounds and texture properties of cereal bars formulated with banana peel flour during storage. Design/methodology/approach Seven cereal bars were produced and stored during 11 months, under vacuum and protected from the light. The total phenolic compounds, the activity antioxidant by ABTS [2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] method, the DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) method expressed as EC50 (concentration required to reduce the original amount of free radicals by 50 per cent) and texture properties were evaluated over the storage period. Findings In general, total phenolic compounds decreased during storage (from 4.19 to 1.11 mg GAE. g-1f.w.). Although the total antioxidant activity (ABTS method) increased during the fifth month, it reduced during storage (from 3.41 to 0.30 µmol TE.g-1f.w.); and the EC50 was not modified in many formulations, though it decreased in other formulations during storage period (from 3913 to 19221 g fruit.g-1 DPPH). The force of rupture began to increase in the fourth month (reaching 62.4 N), and hardness began to increase in the ninth month (reaching 444 N). The formulation and time factors influenced the total phenolic compounds, total antioxidant activity (ABTS method), force of rupture and hardness, while EC50 was only influenced by the formulation (p-value = 0.001). A principal component analysis showed that time had little effect on the most important characteristics considered in description of the cereal bars. Originality/value Cereal bars can be consumed up to the third month of storage, considering the texture of the products. Moreover, the presence of bioactive compounds in cereal bars depends on the addition of banana peel flour, which it contributes to the insertion of total phenolic compounds and total antioxidant activity in cereal bars, aggregating functional properties in these products.


Genetika ◽  
2013 ◽  
Vol 45 (1) ◽  
pp. 87-100 ◽  
Author(s):  
Sladjana Zilic ◽  
Vesna Hadzi-Taskovic-Sukalovic ◽  
Dejan Dodig ◽  
Vuk Maksimovic ◽  
Vesna Kandic

The objective of this study was to determine phenolic compounds and the total antioxidant capacity in the grain of ten bread (T. aestivum L.) and ten durum (T. durum Desf.) wheat genotypes. Soluble free forms of total phenolics, flavonoids, PVPP (polyvinylpolypyrrolidone) bound phenolics, proanthocyanidins and phenolic acids were investigated. In addition, the correlation coefficients between total antioxidant capacities and the concentration of different soluble free phenolic compounds, as well as between soluble free total phenolics and phenolic acids, flavonoids and PVPP bound phenolics were determined. Significant differences in the content of aceton/water extractable total phenolics, PVPP bound phenolics and phenolic acids between and within two wheat species were found. On the average, durum wheat samples had about 1.19-fold higher total phenolic compounds and about 1.5-fold higher PVPP bound phenolics than bread wheat samples. Three phenolic acids, ferulic, caffeic and chlorogenic, were detected in wholemeal bread wheat. Caffeic acid was not found in durum wheat samples whilst ferulic acid was the most abundant. Proanthocyanidins in bread and durum wheat genotypes were not detected. The antioxidant capacity measured as the DPPH radical scavenging activity was similar in wholemeal of bread and durum wheat, however, significant differences were observed among genotypes within species.


2013 ◽  
Vol 41 (2) ◽  
pp. 155-158
Author(s):  
TM Quadery ◽  
F Islam ◽  
M Ahsan ◽  
CM Hasan

A methanolextract of the leaves of Parabaena sagitatta Miers and its petroleum ether, carbon tetrachloride, dichloromethane, ethylacetate and aqueous soluble partitionates were evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH), Folin-Ciocalteau reagent and phosphomolybdenum total antioxidant assays by using butylated hydroxytolune (BHT) and ascorbic acid as standards. The dichloromethane soluble fraction demonstrated the presence of significant amount of phenolic compounds 61.06 ± 0.54 mg GAE/g of extract and also has moderate antioxidant activity IC50 50.62 ± 0.25 ?g/ml. A positive correlation (R2), 0.969 was observed between total phenolic content and total antioxidant activity of P. sagitatta. The general toxicity was determined by brine shrimp lethality bioassay where the dichloromethane LC50DOI: http://dx.doi.org/10.3329/bjb.v41i2.13441 Bangladesh J. Bot. 41(2): 155-158, 2012 (December)


2006 ◽  
Vol 12 (5) ◽  
pp. 385-395 ◽  
Author(s):  
S. Silva ◽  
L. Gomes ◽  
F. Leitão ◽  
A. V. Coelho ◽  
L. Vilas Boas

Extracts of leaves, fruits and seeds of olive tree cultivars of Trás-os-Montes e Alto Douro (Portugal) were analysed by reverse phase HPLC with diode array detection and mass spectrometry (MS). This methodology allowed the identification of some common phenolic compounds, namely, verbascoside, rutin, luteolin-7-glucoside, oleuropein and hydroxytyrosol. Moreover the hyphenation of HPLC with MS enabled the identification of nüzhenide in olive seeds. An oleuropein glucoside was also detected in olive tree leaves. The total phenolic content was determined with the Folin Denis reagent and the total antioxidant activity with the ABTS method. There is a correlation between total antioxidant activity and total phenolic content with the exception of the seed extracts analysed. The apparent high antioxidant activity of seed extracts may be due to nüzhenide, a secoiridoid that is the major phenolic component of olive seeds. These results suggest a possible application of olive seeds as sources of natural antioxidants.


Author(s):  
REGINA ANDAYANI ◽  
FITHRIANI ARMIN ◽  
AINUL MARDHIYAH

Objectives: Garcinia atroviridis Griff. ex T. Anders., Garcinia mangostana L., and Garcinia cowa Roxb. are plants of the genus Garcinia that has been widely used by the community as a food flavoring, spices, and also as a herbal medicinal ingredient. This research aimed to evaluate the total phenolics and antioxidant activity from three species of Garcinia (G. atroviridis Griff. ex T. Anders., G. mangostana L., and G. cowa Roxb.) Methods: The total phenolic content (TPC) of the extracts was estimated as Gallic Acid Equivalent by the Folin-Ciocalteu method. Antioxidant activity was assessed using Ferric Reducing Antioxidant Power assay. Results: The TPC of G. mangostana L. rind extract is higher (31.83±3.70%), than G. cowa Roxb.(4.35±0.17%) and G. atroviridis Griff. ex T. Anders. (2.47±0.42%). Based on the antioxidant activity, G. mangostana L. rind has a higher total antioxidant activity (24.68 μmol Fe(II)/g) than G. cowa Roxb. (18.88±0.12 μM Fe (II)/g and G. atroviridis Griff. ex T. Anders.(17.61±0.05 μM Fe(II)/g). Conclusion: The results showed that G. mangostana L rind extract contains a higher level of TPC and antioxidant activity among the other rinds. The results obtained indicate that the three samples have the potential to be a source of natural antioxidants. Further studies must be carried out to isolate compounds that have antioxidant activity.


2021 ◽  
Vol 42 (5) ◽  
pp. 1347-1354
Author(s):  
M.K. Berwal ◽  
◽  
S.M. Haldhar ◽  
C. Ram ◽  
P.L. Saroj ◽  
...  

Aim: The aim of the study was Identification and quantification of phenolic compounds, total flavonoides content and antioxidant potential of flower buds, foliages, bark and root of a perennial herb, Calligonum polygonoides (Phog) of Thar Desert. Methodology: The methanolic extracts of different plant parts of C. polygonoides were subjected to LC-MS/MS (Waters Acquity UPLC-PDA, TQD) analysis for phenolic identification and spectrophotometric assay of total phenolic and flavonoids content and total antioxidant activity was estimated. Results: A total of 15 phenolic compounds were identified and quantified, among which gallic acid content was abundant, followed by catechin. Besides these, the other major phenolic compounds detected in different plant parts were vanillic, chlorogenic acid, epicatechin, coumeric acid, catechol, vanillic acid, epicatechin and syringic acid. Extremely higher values were recorded for total phenolics, flavonoids and antioxidant capacity. The values for phenol, flavonoids and total antioxidant activity in bark extracts were higher than that of clove extract. Interpretation: The identified phenolic compounds possess high antioxidant potential. These results provide scientific evidence for use of C. polygonoides as safe natural antioxidant compounds in nutraceutical and pharmaceutical industry.


10.5219/1314 ◽  
2020 ◽  
Vol 14 ◽  
pp. 501-509
Author(s):  
Olga Grygorieva ◽  
Olena Vergun ◽  
Svitlana Klymenko ◽  
Mykhailo Zhurba ◽  
Vladimí­ra Horčinová Sedláčková ◽  
...  

The aim of the research is the determination of the total antioxidant activity and the content of phenolic compounds of the leaves of 12 species of non-traditional plants, namely, Amelanchier alnifolia (Nutt.) Nutt. ex M. Roem., Aronia mitschurinii A.K. Skvortsov & Maitul., Castanea sativa Mill., Chaenomeles japonica (Thunb.) Lindl., Cornus mas L., Diospyros kaki L., Diospyros lotus L., Diospyros virginiana L., Lycium barbarum L., Lycium сhinense Mill., Pseudocydonia sinensis (Thouin) C.K. Schneid., Ziziphus jujuba Mill. Total phenolic content was evaluated using the Folin-Ciocalteu reagent assay. Antioxidant activity was measured using two different methods (DPPH – 2,2-diphenyl-1-picrylhydrazyl, MRAP – molybdenum reducing antioxidant power). Significant variability was observed in phenolic compounds content and total antioxidant activity. Total polyphenol content ranged from 38.02 (Z. jujuba) to 80.58 (C. sativa) mg GAE.g-1 DM, total flavonoid content from 22.47 (P. sinensis) to 54.61 (L. barbarum) mg QE.g-1 DM and phenolic acids content from 3.51 (A. mitschurinii) to 24.67 (Ch. japonica) mg CAE.g-1 DM. All tested samples exhibited DPPH• radical scavenging activities with values from 6.92 (A. mitschurinii) to 9.0 (C. mas) mg TEAC.g-1 DM. Antioxidant activity by molybdenum reducing antioxidant power method ranged from 109.43 (A. mitschurinii) to 322.95 (C. mas) mg TEAC.g-1 DM. Differences between the species of non-traditional plants were significant in all observed parameters. Obtained results of phytochemical composition demonstrated the possibility of leaves' use of non-traditional plants as sources of valuable bioactive compounds with health-promoting and disease-preventing properties.


2001 ◽  
Vol 71 (3) ◽  
pp. 136-142
Author(s):  
Dan Razvan Popoviciu ◽  
Ticuta Negreanu-Pirjol ◽  
Ludmila Motelica ◽  
Bogdan Stefan Negreanu Pirjol

Cotoneaster horizontalis Decne. and Cotoneaster microphyllus Wall. ex Lindl. species are two creeping bushes, commonly used as ornamental plants in gardens and parks. The aim of this paper was to assess the concentrations of some classes of bioactive compounds classes, carotenoids, flavonoids and total phenolic compounds, in fresh fruits of these species. Carotenoids and flavonoids were determined through acetone and methanol extraction followed by spectrophotometry. For total phenolics, methanol extraction and a spectrophotometric Folin-Ciocalteu method was used. The total antioxidant capacity was quantified through photochemiluminescence method by comparison with the standard substance used for calibration, Trolox� as tocopherol analogue (ACL procedure) using Photochem apparatus, Analytik Jena AG, Germany. Average values found in Cotoneaster horizontalis and Cotoneaster microphyllus fruit tissue were 380.63 mg/kg, respectively 179.63 mg/kg, carotenoids; 8036.07 mg/kg, respectively 6888.06 mg/kg flavonoids; and 16342.06 mg/kg GAE, respectively 18631.35 mg/kg GAE total phenolic compounds. These values are comparable to those found in other wild and cultivated related Rosaceae, including domestic rowans. Cotoneaster microphyllus fruits emphasized an increased antioxidant activity (up to 39.69 μmol Trolox equivalent/g dry weight).


2017 ◽  
Vol 6 (1) ◽  
pp. 25-31 ◽  
Author(s):  
Buanasari Buanasari ◽  
Willy Tirza Eden ◽  
Ayu Ina Sholichah

The antioxidant has an activity to neutralize free radical compound that the body needs to avoid damage cells and tissues. Phenolic is one of the compounds that have an antioxidant activity. The influences of ultrasonic-assisted extraction (UAE) and microwave-assisted extraction (MAE) conditions on phenolic compounds of Parkia speciosa Hassk. leaves were investigated. The effects of temperature (40C, 50C, 60C and 70C), time (10, 30 and 50 minutes) and material-solvent ratio (1:10, 1:13, 1:15 ) were evaluated based on the yield, total phenolic content (TPC) and antioxidant activity. The result showed that the highest yield (15.82%) was obtained at 1:15 (w/w) of material-solvent ratio, 50C of temperature and 50 minutes of extraction time for MAE. The highest yield of UAE is 15.53% that sample was obtained at 1:13 (w/w) of material-solvent ratio, 60C of optimal temperature and 30 minutes extraction time. The highest IC50 of UAE method extract was 52.55 ppm, while the extract obtained using MAE method was 50.44 ppm. UAE is more stable at higher temperatures. Time and solvent which was used more efficient than MAE. Extract of petai leaves (Parkia speciosa Hassk.) were very potential to be used as a source of natural antioxidants because they have IC50 values from 41.39 to 66.00 ppm. Its antioxidants capacity is ranged from strong to very strong capacity.


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