scholarly journals Profiling of antioxidant potential and phytoconstituents of Plantago coronopus

2016 ◽  
Vol 77 (3) ◽  
pp. 632-641 ◽  
Author(s):  
C. G. Pereira ◽  
L. Custódio ◽  
M. J. Rodrigues ◽  
N. R. Neng ◽  
J. M. F. Nogueira ◽  
...  
Keyword(s):  
Amino Acids ◽  
High Performance ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Essential Amino Acids ◽  
Bioactive Molecules ◽  
It Use ◽  
Nitric Oxide Radical ◽  
Plantago Coronopus

Abstract The halophyte species Plantago coronopus has several described ethnomedicinal uses, but few reported biological activities. This work carried out for the first time a comparative analysis of P. coronopus organs in terms of phenolic composition and antioxidant activity of organic and water extracts from roots, leaves and flowers. The leaves contents in selected nutrients, namely amino acids and minerals, are also described. Roots (ethyl acetate and methanol extracts) had the highest radical scavenging activity (RSA) towards 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radicals, while leaves (hexane extract) had higher RSA on nitric oxide radical and iron chelating ability. High performance liquid chromatography (HPLC) analysis identified eighteen phenolics from which salicylic acid and epicatechin are here firstly described in Plantago species. Leaves had mineral levels similar to those of most vegetables, proving to be a good source for elements like calcium, sodium, iron and magnesium, and also for several of the essential amino acids justifying it use as food. Our results, especially those regarding the phenolics composition, can explain the main traditional uses given to this plantain and, altogether, emphasize the potential of P. coronopus as a source of bioactive molecules particularly useful for the prevention of oxidative stress-related diseases.

scholarly journals Changes in Amino Acid Profiles and Bioactive Compounds of Thai Silk Cocoons as Affected by Water Extraction

Molecules ◽  
2021 ◽  
Vol 26 (7) ◽  
pp. 2033
Author(s):  
Chuleeporn Bungthong ◽  
Sirithon Siriamornpun
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Silk Protein ◽  
Extraction Time ◽  
Antioxidant Power ◽  
Silk Cocoon ◽  
Amino Acid Profiles

Silk proteins have many advantageous components including proteins and pigments. The proteins—sericin and fibroin—have been widely studied for medical applications due to their good physiochemical properties and biological activities. Various strains of cocoon display different compositions such as amino-acid profiles and levels of antioxidant activity. Therefore, the objectives of this study were to find a suitable silk protein extraction method to obtain products with chemical and biological properties suitable as functional foods in two strains of Bombyx mori silk cocoon (Nangsew strains; yellow cocoon) and Samia ricini silk cocoon (Eri strains; white cocoon) extracted by water at 100 °C for 2, 4, 6 and 8 h. The results showed that Nangsew strains extracted for 6 h contained the highest amounts of protein, amino acids, total phenolics (TPC) and total flavonoids (TFC), plus DPPH radical-scavenging activity, ABTS radical scavenging capacity, and ferric reducing antioxidant power (FRAP), anti-glycation, α-amylase and α-glucosidase inhibition. The longer extraction time produced higher concentrations of amino acids, contributing to sweet and umami tastes in both silk strains. It seemed that the bitterness decreased as the extraction time increased, resulting in improvements in the sweetness and umami of silk-protein extracts.

scholarly journals Phytochemical Traits and Biological Activity of Eryngium amethystinum and E. alpinum (Apiaceae)

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 364
Author(s):  
Dario Kremer ◽  
Marijana Zovko Končić ◽  
Ivan Kosalec ◽  
Iztok Jože Košir ◽  
Tanja Potočnik ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
High Performance ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Dilution Assay ◽  
Germacrene D ◽  
Antimicrobial Evaluation ◽  
Antibacterial And Antifungal

Eryngium amethystinum L. is a wild vegetable used in Croatia. Both E. amethystinum and E. alpinum L. are decorative plants that can also be used in horticulture. To find out the hidden qualities of these two species, the following biological activities were studied: essential oil (EO) composition, analyzed by gas chromatography and gas chromatography with mass spectrometry (GC, GC–MS); phenolic compound content (PC), analyzed by high performance liquid chromatography (HPLC); total phenols as well as total flavonoids, analyzed by ultraviolet–visible spectrophotometry (UV/Vis); antioxidants, analyzed by 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity (DPPH), b-carotene-linoleic acid assay, chelating activity and reducing power of the extracts; and antimicrobial evaluation by micro-dilution assay and inhibition of Candida albicans blastospore germination. The major constituents of EO were β-caryophyllene (15.2%), α-pinene (10.2%) and 2,3,6-trimethylbenzaldehyde (9.3%) for E. amethystinum and caryophyllene oxide (27.9%), bicyclogermacrene (13.2%) and germacrene D (8.2%) for E. alpinum. The methanol extracts of both species showed a broad spectrum of antibacterial and antifungal activity with minimum inhibitory concentrations (MIC) less than or equal to 1.944 and 1.11 mg/mL, respectively.

scholarly journals Metabolomic Profiling and Antioxidant, Anticancer and Antimicrobial Activities of Hyphaene thebaica

Processes ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 266
Author(s):  
Ghada A. Taha ◽  
Ibrahim B. Abdel-Farid ◽  
Hassan A. Elgebaly ◽  
Usama A. Mahalel ◽  
Mohamed G. Sheded ◽  
...  
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Methanolic Extracts ◽  
Carcinoma Cell Lines ◽  
Metabolomic Profiling ◽  
Total Antioxidant ◽  
Positive Correlations

This study investigated the metabolic content and biological activities of Hyphaene thebaica leaves, male parts and fruits methanolic extracts. The phytochemical constituents were determined, and multivariate data analysis discriminated the evaluated three parts into three groups according to the content of phenolics, flavonoids, flavonols, saponins, anthocyanins and tannins. High-performance liquid chromatography (HPLC) profiling of polyphenols revealed the dominance of catechins, rutin and apigenin-7-glucosides in leaves, protocatechuin, p-hydroxybenzoic, syringic, vanillic, rosmarinic, p-coumaric acids and chrysin in male parts, and chlorogenic acid in fruits. Leaves and male parts showed stronger free radical scavenging activity than the fruits. Positive correlations between total antioxidant capacity and carbohydrates, phenolics and flavonols were observed. The three extracts exhibited potent anti-cancer activity against liver and lung carcinoma cell lines. All extracts exhibited antibacterial activity, while only fruits showed antifungal efficacy. In conclusion, H. thebaica leaves, male parts and fruits contain a variety of phytochemicals with antioxidant, anticancer and antimicrobial activities.

scholarly journals A New Macrolactone, Racemolide Along With Seven Known Compounds With Biological Activities From Mangrove Plant,Lumnitzera racemosa

2019 ◽  
Vol 14 (6) ◽  
pp. 1934578X1986125
Author(s):  
Ahmed Gomaa Gomaa Darwish ◽  
Mamdouh Nabil Samy ◽  
Sachiko Sugimoto ◽  
Hideaki Otsuka ◽  
Katsuyoshi Matsunami
Keyword(s):  
High Performance ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Hepatoprotective Activity ◽  
Dpph Radical ◽  
Mangrove Plant ◽  
Anti Oxidant ◽  
Dpph Radical Scavenging ◽  
Lumnitzera Racemosa

One new macrolide, racemolide (1), was isolated from an MeOH extract of the leaves of Lumnitzera racemosa with 7 known compounds (2-8) by various chromatographic techniques via silica gel, octadecylsilyl column chromatography and high-performance liquid chromatography. The structure of the new compound was determined through a combination of spectroscopic and chemical analyses. The isolated compounds were evaluated for their antileishmanial, hepatoprotective, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities. Compound 1 showed potent antileishmanial activity at inhibition % value of 67.6 ± 1.24% compared with the clinical drug miltefosine (93.3 ± 4.39%). Compounds 1 and 5 possessed moderate hepatoprotective activity against acetaminophen (APAP)-induced hepatotoxicity using human HepG2 cells at protection % value of 21.70 ± 1.97% and 27.5 ± 1.41%, respectively, compared with the standard glycyrrhizin (69.50 ± 3.53%). Compounds 4, 6, 7, and 8 had high DPPH radical scavenging activity (91.10 ± 4.25, 91.53 ± 0.05, 91.91 ± 0.09, and 86.48 ± 2.87%, respectively), while compounds 3 and 5 showed moderate (45.38 ± 3.62% and 62.20 ± 1.47%, respectively), comparable with the standard trolox (89.21 ± 5.88%). These results suggest that the new compound, racemolide (1), and known compounds (3-8) are valuable as promising antileishmanial, hepatoprotective, and anti-oxidant agents.

scholarly journals Synthesis and Evaluation of the Antibacterial and Antioxidant Activities of Some Novel Chloroquinoline Analogs

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Bayan Abdi ◽  
Mona Fekadu ◽  
Digafie Zeleke ◽  
Rajalakshmanan Eswaramoorthy ◽  
Yadessa Melaku
Keyword(s):  
Molecular Docking ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Dna Gyrase ◽  
Inhibition Zone ◽  
Bioactive Molecules ◽  
Scavenging Activity ◽  
Good Activity ◽  
E Coli

Quinoline heterocycle is a useful scaffold to develop bioactive molecules used as anticancer, antimalaria, and antimicrobials. Inspired by their numerous biological activities, an attempt was made to synthesize a series of novel 7-chloroquinoline derivatives, including 2,7-dichloroquinoline-3-carbonitrile (5), 2,7-dichloroquinoline-3-carboxamide (6), 7-chloro-2-methoxyquinoline-3-carbaldehyde (7), 7-chloro-2-ethoxyquinoline-3-carbaldehyde (8), and 2-chloroquinoline-3-carbonitrile (12) by the application of Vilsmeier–Haack reaction and aromatic nucleophilic substitution of 2,7-dichloroquinoline-3-carbaldehyde. The carbaldehyde functional group was transformed into nitriles using POCl3 and NaN3, which was subsequently converted to amide using CH3CO2H and H2SO4. The compounds synthesized were screened for their antibacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Streptococcus pyogenes. Compounds 6 and 8 showed good activity against E. coli with an inhibition zone of 11.00 ± 0.04 and 12.00 ± 0.00 mm, respectively. Compound 5 had good activity against S. aureus and P. aeruginosa with an inhibition zone of 11.00 ± 0.03 mm relative to standard amoxicillin (18 ± 0.00 mm). Compound 7 displayed good activity against S. pyogenes with an inhibition zone of 11.00 ± 0.02 mm. The radical scavenging activity of these compounds was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH), and compounds 5 and 6 displayed the strongest antioxidant activity with IC50 of 2.17 and 0.31 µg/mL relative to ascorbic acid (2.41 µg/mL), respectively. The molecular docking study of the synthesized compounds was conducted to investigate their binding pattern with topoisomerase IIβ and E. coli DNA gyrase B. Compounds 6 (−6.4 kcal/mol) and 8 (−6.6 kcal/mol) exhibited better binding affinity in their in silico molecular docking against E. coli DNA gyrase. The synthesized compounds were also found to have minimum binding energy ranging from −6.9 to −7.3 kcal/mol against topoisomerase IIβ. The SwissADME predicted results showed that the synthesized compounds 5–8 and 12 satisfy Lipinski’s rule of five with zero violations. The ProTox-II predicted organ toxicity results revealed that all the synthesized compounds were inactive in hepatotoxicity, immunotoxicity, mutagenicity, and cytotoxicity. The findings of the in vitro antibacterial and molecular docking analysis suggested that compound 8 might be considered a hit compound for further analysis as antibacterial and anticancer drug. The radical scavenging activity displayed by compounds 5 and 6 suggests these compounds as a radical scavenger.

Essential oils and alcohol extracts of Northwest Tunisia M. communis and M. spicata: phytocompounds, antioxidant and antibacterial activities

2021 ◽  
Vol 17 ◽  
Author(s):  
Saida Medjeldi ◽  
Nora Benrachou ◽  
Sabrina Bouchelaghem ◽  
Sélim Jallouli ◽  
Nadia Farès ◽  
...  
Keyword(s):  
Volatile Compounds ◽  
Pathogenic Bacteria ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Activities ◽  
Bioactive Molecules ◽  
Diffusion Method ◽  
Mentha Spicata ◽  
Promising Source

Background: Nowadays, the conventional chemical compounds used in the food, pharmaceutical, and cosmetic sectors have proven to be less efficient from an economic and health point of view. Objective: Research has therefore focused on the isolation of bioactive molecules from plants or microorganisms to remedy this situation. Methods: The study focused on the leaf’s volatile compounds determination from Myrtus communis (McEOs) and Mentha spicata (MsEOs) as well as the evaluation of phytochemical fractions and biological activities from their methanol extracts (McME and MsME). Results: Phenolic compounds analysis revealed interesting concentrations of total phenols, flavonoids, and condensed tannins. The antibacterial activity tested on seven pathogenic bacteria has to be effectuated by the gel diffusion method. The extracts showed much better DPPH radical scavenging activity than the volatile compounds. The McEOs and MsEOs profiles analyzed by CG-MS showed a mixture of several compounds with a predominance of 1,8-Cineole (48.1%) and α-pinene (39.02%) in the case of McEOs. In de case of MsEOs, the predominant compounds were D-Carvone (78.79%), dihydrocarvone (6.16%), and 1.8 Cineole (2.33%). It emerges from this study that the M. communis and M. spicata leaves, rich in phenolic and terpenic compounds constitute a promising source of diversified biological activities and can be used in agro-food, cosmetic, and pharmaceutical sectors.

scholarly journals FORMULATION, CHARACTERIZATION AND EVALUATION OF ANTI-INFLAMMATORY AND ANTI-ANGIOGENIC ACTIVITIES OF MEMECYLAENE NANOEMULSION

2018 ◽  
Vol 8 (5-s) ◽  
pp. 126-131
Author(s):  
ND Rekha ◽  
Dattatri K. Nagesha ◽  
PH Rajasree ◽  
N Shruthi
Keyword(s):  
Zeta Potential ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
In Vivo Model ◽  
Particle Size Range ◽  
Nitric Oxide Radical ◽  
Anti Oxidant ◽  
Anti Inflammatory

The present study was undertaken to formulate and evaluate the anti-inflammatory, anti-oxidant and anti-angiogenic activities of nanoemulsion of Memecylaene.  Memecylaene was isolated from the leaves of Memecylon malabaricum by using various chromatographic methods. An oil-in-water (O/W) nanoemulsion of Memecylaene was formulated by sonication method using sunflower oil (oil phase), Tween 80 (Surfactant) and Ethanol (co-surfactant). The prepared nanoemulsion was characterized for its droplet size, poly dispersity index and zeta potential. Stability studies were performed and the nanoemulsions were subjected to different biological activities. The formulated nanoemulsion had a particle size range of 52.02 nm to 59.47 nm and zeta potential of -1.27 mV. The enhanced activity of Memecylaene, encapsulated in O/W emulsions is evidenced by the inhibition of phospholipase (PLA2) enzyme and H+, K+ -ATPase and thus showing anti-inflammatory and anti-secretagogues effects. The in vitro anti-oxidant activity was evaluated by DPPH radical and Nitric oxide radical scavenging activity. Further, the inhibition of the growth of neo vessels formation in the in-vivo model system of chick chorioallantoic membrane (CAM) assay, which is angiogenesis dependent, was also observed. The above findings would help in understanding the putative potential of Memecylaene-loaded nanoemulsion as a therapeutic agent. Keywords: Anti-angiogenesis, Anti-oxidant, Gastric (H+ K+), Memecylaene, Nanoemulsion, Phospholipase A2 (PLA2).

scholarly journals Enhancement of Phenolics Content and Biological Activities of Longan (Dimocarpus Longan Lour.) Treated with Thermal and Aging Process

2021 ◽  
Author(s):  
Preaploy Hong-in ◽  
Waranya Neimkhum ◽  
Chanun Punyoyai ◽  
Suwannee Sri ◽  
Wantida Chaiyana
Keyword(s):  
High Performance ◽  
Antioxidant Activities ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Aluminium Chloride ◽  
Chemical Compositions ◽  
Dimocarpus Longan ◽  
Phenolics Content

Abstract This study is the first to successfully produce novel black Dimocarpus longan by undergoing thermal and ageing process. Pericarp, aril, and seed of black D. longan were macerated in 95% v/v ethanol. Their chemical compositions were investigated by Folin–Ciocalteu assay, aluminium chloride assay, and high-performance liquid chromatography. Antioxidant activities were evaluated in terms of radical scavenging and iron (III) reducing capacity. Enzyme inhibitory assay was used to evaluate the hyaluronidase inhibition. Black D. longan seed extract contained the significantly highest content of flavonoids and phenolic compounds (p < 0.05). Each gram of the extract contained 53.6 ± 0.9 mg of gallic acid, 19.8 ± 2.9 mg of corilagin, and 24.5 ± 0.7 mg of ellagic acid. This extract was the most potent anti-hyaluronidase and antioxidant with the strongest free radical scavenging activity and reducing power. Therefore, it was proposed as functional food and further used in the pharmaceutical or cosmeceutical industries.

The impact of fixation method on the biochemical characteristics of hawk tea (Litsea coreana)

2020 ◽  
Vol 16 (8) ◽  
Author(s):  
Qing Xu ◽  
Yuanjing Zhou ◽  
Jihong Wang
Keyword(s):  
Amino Acids ◽  
Sensory Quality ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Essential Amino Acids ◽  
Boiling Water ◽  
Fixation Method ◽  
Biochemical Characteristics ◽  
Fixation Techniques ◽  
The Impact

AbstractFixation is one of the critical steps in tea processing and can cause various physico-chemical changes, thus affecting tea quality. There are many tea processing techniques, but their influence on quality remains unclear. Here, we studied the impact of different fixation methods, including for 6 or 10 min by machine, by iron pan, or by boiling water, on the biochemical characteristics of hawk tea. The results showed that tea fixation technique had a significant influence on the sensory quality, biochemical components, and antioxidant activity of the tea. Compared to other fixation techniques, fixation by boiling water produced a more nutritious hawk tea with stronger DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging activity (EC50 = 6.56 μg/mL). Processing with boiling water also resulted in a better sensory quality with a unique sweet aftertaste. This tea also contained more polyphenols (19.60%), total amino acids (21,219 mg/100 g), essential amino acids (10,564 mg/100 g), and trace elements (5873.13 mg/kg). These results can guide the development of hawk tea products.

scholarly journals Characterization and Biological Activity of a Novel Exopolysaccharide Produced By Pediococcus Pentosaceus SSC-12 From Silage

2021 ◽  
Author(s):  
Yang Fan ◽  
Xinqin Li ◽  
Rong Tian ◽  
Ruxue Tang ◽  
Jianguo Zhang
Keyword(s):  
Hydroxyl Radical ◽  
High Performance ◽  
Biological Activities ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Exchange Chromatography ◽  
Pediococcus Pentosaceus ◽  
Pharmaceutical Industries ◽  
Radical Scavenging Ability

Abstract Background: The exopolysaccharides (EPS) produced by lactic acid bacteria (LAB) are widely used in various fields because of their safety and various biological activities. In this study, we extracted and characterized the composition as well as antioxidant and antibacterial activities of EPS from Pediococcus pentosaceus SSC-12 isolated from the silage.Results: The LAB strain SSC-12 was screened and identified as Pediococcus pentosaceus, based upon 16S rDNA gene sequencing and Neighbor Joining (NJ) phylogenetic analysis. The analysis of the EPS production kinetics results of SSC-12 showed that the EPS production reached the maximum at 20 h of culture. High-performance anion exchange chromatography (HPAEC) analysis showed that the EPS produced by SSC-12 was a heteropolysaccharide comprising glucose (42.6 %), mannose (28.9 %), galactose (16.2 %), arabinose (9.4 %) and rhamnose (2.9 %). The EPS had good antioxidant activity, especially hydroxyl radical scavenging activity. When the concentration of the EPS produced by SSC-12 (SSC-12 EPS) was 10 mg/mL, its 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging ability, hydroxyl radical scavenging ability, superoxide scavenging ability, and reduction ability were 77.4 %, 97.5 %, 77.5 % and 1.3, respectively. At 10 mg/mL，SSC-12 EPS completely killed Staphylococcus aureus GDMCC 1.1220 and substantially inhibited the growth of Salmonella enterica subsp. enterica GDMCC 1.345; however, it had a weak inhibitory effect on Listeria monocytogenes GDMCC 1.347. Conclusions: Due to its strong antioxidant and antibacterial properties, EPS produced by LAB strain SSC-12 have potential application as a bioactive product in the feed, food, and pharmaceutical industries.

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