scholarly journals "BAIXADEIROS" HORSES: PREVALENCE OF ANTI-Trypanosoma spp. AND ANTI-Leishmania spp. ANTIBODIES

2018 ◽  
Vol 19 (0) ◽  
Author(s):  
Fernanda Pinto Ferreira ◽  
Eloiza Teles Caldart ◽  
Danilo Rodrigues Barros Brito ◽  
Daniel Praseres Chaves ◽  
João Luis Garcia ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Control Measures ◽  
Statistical Association ◽  
Subsequent Separation ◽  
Leishmania Spp ◽  
Horse Blood ◽  
Collection Period ◽  
Immunoenzymatic Assays ◽  
Significant Statistical Association

Abstract A total of 138 horse blood samples were collected from 2012 to 2013 for the subsequent separation of serum collection. For detection of anti-Leishmania spp. and anti-Trypanosoma spp. antibodies, indirect immunofluorescence reactions and immunoenzymatic assays were performed. Samples with titers ≥40 were considered positive in the indirect immunofluorescence (IFAT), and the cut off for the enzyme immunoassay (ELISA) was calculated with three standard deviations. When tested for Trypanosoma spp., 50.37% (68/135) of the samples were ELISA reagents, 18.84% (8/136) were positive for IFAT and 2.89% (4/138) for both tests. A significant statistical association was observed for the municipality (p = 0.013) and collection period (p = 0.042) in the ELISA. When tested for Leishmania spp., 25.4% (35/138) of the samples were ELISA reagents, 13.00% (18/138) were positive for the IFAT and 4.34% (6/138) were positive for two tests, there were no variables associated with seropositivity. A statistical association (p = 0.0034) was observed between the presence of anti-Trypanosoma spp. and anti-Leishmania spp. antibodies when the ELISA was used. The results suggest that this horse breed is in contact with leishmaniasis and trypanosomiasis, which demonstrates the need for a more accurate investigation into the real role of horses in these diseases to assist in disease control measures.

scholarly journals Molecular investigation and geographical distribution of Leishmania spp infection in stray and owned cats (Felis catus) in Thessaly, central Greece

2018 ◽  
Vol 68 (1) ◽  
pp. 27 ◽  
Author(s):  
A. GIANNAKOPOULOS ◽  
C. N. TSOKANA ◽  
E. PAPADOPOULOS ◽  
V. SPYROU ◽  
D. C. CHATZOPOULOS ◽  
...  
Keyword(s):  
Geographical Distribution ◽  
Nested Pcr ◽  
Living Conditions ◽  
Control Measures ◽  
Geographical Information ◽  
Endemic Region ◽  
Central Greece ◽  
Molecular Investigation ◽  
Leishmania Spp

In this study we aimed to determine the prevalence of Leishmania spp infection and to report the geographical distribution of Leishmania spp infected cats from Thessaly, central Greece, an endemic region for canine and human leishmaniosis. Blood samples were collected from a total of 150 cats (34 stray and 116 owned) from the area of Thessaly, i.e. Larissa (n=6), Volos (n=94), Trikala (n=30) and Karditsa (n=20). Data on signalment and living conditions were registered for each cat. The samples were tested by Internal Transcribed Spacer 1 nested PCR (ITS1 nested PCR). Geographical Information System (GIS) was used to define the geographical distribution of the Leishmania spp infected cats in relation to the land uses and the altitude. In total, 13.3% (95% CI: 8.3-19.8) of the sampled cats were found positive for Leishmania spp DNA. More precisely, the results are 12.8% (95% CI 6.8-21.2) in Volos, 20% (95% CI 6.8-21.2) in Trikala and 10% (95% CI 12-31.7) in Karditsa while none of the six cats examined from the region of Larissa was found PCR positive. The Leishmania spp infected cats were found in artificial surfaces and associated areas and in cultivated and managed areas with a mean altitude of 81,7 m above sea level (range 14 - 225 ± 51.57 SD). No significant correlation was found between Leishmania spp infection and signalment or living conditions, thus suggest ing that the infection is uniformly distributed among the screened feline population. The findings of this study along with the occurrence of competent vectors of Leishmania infantum in Thessaly, support the hypothesis that cats may serve as a reservoir for Leishmania spp in this region and highlight the need for surveillance in feline population and implementation of control measures. However, the epidemiological role of cats in the maintenance and transmission of Leishmania spp has yet to be defined and deserves further investigation.

Temperament and Loving-Styles in College Women: Associations with Eating Attitudes

1999 ◽  
Vol 84 (1) ◽  
pp. 305-311 ◽  
Author(s):  
John Worobey
Keyword(s):  
Interpersonal Relationships ◽  
Eating Behaviors ◽  
Eating Attitudes ◽  
Statistical Association ◽  
Love Styles ◽  
Self Perceptions ◽  
Oral Control ◽  
Food Preoccupation ◽  
Significant Statistical Association

The purpose of the present study was to examine the associations of temperament and love attitudes with eating behaviors in 190 college-aged nonclinical women who completed a survey that included measures of temperament, loving-style, and eating attitudes. Certain temperament and loving-style variables showed significant statistical association with scores on eating attitudes. Specifically, both obsessive and game-playing love-styles were related to the Dieting and Bulimia–Food Preoccupation dimensions of the eating scales, while temperamental fear and anger were related to bulimia and oral control. The role of interpersonal relationships and self-perceptions of temperament may provide a useful perspective for understanding the etiology of eating disorders.

scholarly journals Tourism Impacts, Tourism-Phobia and Gentrification in Historic Centers: The Cases of Málaga (Spain) and Gdansk (Poland)

2021 ◽  
Vol 13 (1) ◽  
pp. 408
Author(s):  
Fernando Almeida-García ◽  
Rafael Cortés-Macías ◽  
Krzysztof Parzych
Keyword(s):  
Comparative Research ◽  
Control Measures ◽  
Tourism Impacts ◽  
Tourist Destinations ◽  
Tourist Flows ◽  
Tourist Accommodation ◽  
And Control ◽  
The Impact ◽  
Urban Characteristics

This study analyzes the role of residents in urban tourist destinations affected by the increase in tourist flows, which have generated various problems such as tourism, gentrification and the emergence of tourism as a threat to residents. The role of residents in tourist destinations has not been analyzed regularly during the development process of destinations. We study two cases of historic centers in European cities, with the aim of comparing tourism problems, which are common to most European urban destinations. This study was conducted by administering surveys amongst residents of these historic centers (378 in Málaga, Spain, and 380 in Gdansk, Poland). These cities show a similar demographic size and urban characteristics. This is the first comparative research on tourism-phobia and gentrification in destinations, a field of analysis that is still not studied much. We develop specific scales to measure gentrification and tourism-phobia; moreover, we study the impact of some tourist problems that affect residents (noise, dirt, occupation of public spaces, etc.), and we show the spatial distribution of tourism-phobia. The same analysis instruments are used for both cities. The results of this study show that the tourism-phobia situation is different in the analyzed destinations. It is more intense in the case of Málaga than in Gdansk. The two historic centers are especially affected by the processes of increased tourist flows and the growth of new forms of tourist accommodation. The research results show that the residents’ annoyance caused by tourism gentrification is more intense than tourism-phobia. Both case studies highlight the residents’ complaints regarding the inadequate management of problems by public stakeholders and control measures.

Prevalence of Linguatula sp., a food-borne zoonotic aberrant arthropod, in river buffaloes slaughtered at Tabriz slaughterhouse, Iran

2020 ◽  
Author(s):  
Nasser Hajipour ◽  
Jennifer Ketzis ◽  
Parviz Hassanzadeh
Keyword(s):  
Infection Rate ◽  
Statistical Association ◽  
Intermediate Hosts ◽  
Mesenteric Lymph Nodes ◽  
Intensity Of Infection ◽  
Mesenteric Lymph ◽  
Food Borne ◽  
Different Seasons ◽  
Significant Statistical Association ◽  
Age And Sex

Abstract Background Canids and herbivores are the definitive and intermediate hosts of Linguatula sp., respectively. Methods Mesenteric lymph nodes (n=32 525) were randomly collected from 7585 buffaloes from July 2016 to July 2019 and examined macroscopically. Results Results showed that 388 (5.11%) buffaloes were infected. The intensity of infection was determined to be 3.07±0.07. Significant statistical association was identified between infection rate and age and sex. Although there were no significant differences in the infection rate over different seasons, the highest infection rate was observed in autumn. Conclusions These data highlight the importance of inspection at slaughter.

scholarly journals Characteristics of Indoor Flood Fatalities: Evidence from Greece

2021 ◽  
Vol 13 (15) ◽  
pp. 8612
Author(s):  
Michalis Diakakis ◽  
Katerina Papagiannaki
Keyword(s):  
Building Material ◽  
Human Life ◽  
Older Individuals ◽  
Statistical Association ◽  
Flood Events ◽  
Building Collapse ◽  
Factors Influencing ◽  
Flooding Events ◽  
Practical Implications ◽  
Significant Statistical Association

Despite the important advances in flood forecasting and protection, floods remain one of the most lethal types of natural hazards. Previous works have explored several factors influencing the risks of flooding to human life and health. However, there is limited research and understanding on indoor flood fatalities and the circumstances under which they occur. This study explores victim-, building-, and situation-related characteristics in order to provide a better understanding of the conditions that lead to flood-related indoor deaths, exploiting a fatality database developed for Greece (1960–2020). The correlation analysis showed that indoor victims, compared with outdoor ones, tend to be older individuals, with high percentages of disabilities. A significant statistical association of the building material, roof type, and distance from the river with the building collapse was also found. The profile of the buildings in which flood fatality occurred was further compared with that of neighboring non-fatal buildings that were inhabited during the flood events. The statistical results indicated that the buildings with a fatality occurrence are mostly single-storey structures, made from masonry as the main building material. The findings have practical implications in risk communication and mitigation in terms of identifying the specific populations, circumstances, settings, and mechanisms that lead to dangerous indoor situations during flooding events.

scholarly journals Seroprevalence and risk factors associated with infection by Neospora caninum of dairy cattle in the state of Alagoas, Brazil

2012 ◽  
Vol 32 (10) ◽  
pp. 1009-1013 ◽  
Author(s):  
Maria E. Sousa ◽  
J.N. Porto Wagnner ◽  
Pedro P.F. Albuquerque ◽  
Orestes L. Souza Neto ◽  
Eduardo B. Faria ◽  
...  
Keyword(s):  
Risk Factors ◽  
Dairy Cattle ◽  
Indirect Immunofluorescence ◽  
Neospora Caninum ◽  
Infection Risk ◽  
Control Measures ◽  
Risk Indicators ◽  
Blood Samples ◽  
Factors Associated ◽  
Serological Investigation

The objective of this study was to investigate the prevalence of anti-Neospora caninum antibodies in cattle from milk producing farms of the microregion of Batalha, state of Alagoas, Brazil, as well as to identify the risk factors associated with the infection. Blood samples were collected from 1,004 cattle of 17 farms for the serological investigation regarding the presence of anti-N. caninum antibodies by the Indirect Immunofluorescence Reaction Technique (IMRT). From the total amount of samples analyzed, 77/1,004 (7.67%) were positive and 927/1,004 (92.33%) were negative. The logistical regression identified that cattle from farms without consortium breeding have an infection risk 6.33 (p<0.001; C.I. 2.89-13.10) times higher than cattle from farms with that type of breeding. Cattle from farms where the aborted fetuses are not adequately buried have an infection risk 3.04 (p<0.001; C.I. 1.64-5.63) times higher than cattle from farms with adequate destination of these fetuses. Infection by N. caninum occurs in cattle of the investigated region. The factors identified in our study can be used as risk indicators, so that control measures could be implemented to avoid infection by N. caninum in the herds of this region.

scholarly journals Seroprevalence and risk factors for canine visceral leishmaniasis in the endemic area of Dias D'Ávila, State of Bahia, Brazil

2010 ◽  
Vol 43 (4) ◽  
pp. 400-404 ◽  
Author(s):  
Laura Cristina Pinho de Oliveira ◽  
Rondinelli Ribeiro de Araújo ◽  
Carlos Roberto Alves ◽  
Eliame Mouta-Confort ◽  
Jorge Alberto López ◽  
...  
Keyword(s):  
Visceral Leishmaniasis ◽  
Indirect Immunofluorescence ◽  
Close Contact ◽  
Statistical Significance ◽  
Domestic Dogs ◽  
Mixed Breed ◽  
Rural Zone ◽  
Normochromic Anemia ◽  
Canine Infection ◽  
Immunoenzymatic Assays

INTRODUCTION: Visceral leishmaniasis (VL) is an important zoonosis in relation to public health systems. Dogs are the main domestic reservoir. This study aimed to investigate occurrences of canine VL in Dias D'Ávila, State of Bahia, Brazil. METHODS: The prevalence was evaluated by means of clinical and laboratory tests on a population of 312 domestic dogs from 23 localities in this municipality, using indirect immunofluorescence and immunoenzymatic assays. RESULTS: Among the animals examined, 3.2% and 6.7% showed signs of VL, confirmed by indirect immunofluorescence and immunoenzymatic assays, respectively, with a distribution of 29.9% (24 dogs) in the rural zone and 4.9% (288 dogs) in the urban zone (p = 0.001). The clinical evaluation on seropositive dogs showed both asymptomatic animals (2.4%) and symptomatic animals (47.6%), along with other abnormalities (e.g. normocytic and normochromic anemia, with leukocytosis and thrombocytopenia). Observations relating to phenotypic characteristics (e.g. sex, age, breed and hair) did not present statistical significance, although high seropositivity among male, short-haired and mixed-breed dogs was observed. CONCLUSIONS: The findings showed that VL was a predominantly rural zoonosis and that close contact between poultry and domestic dogs significantly increased the risk of canine infection in this region.

scholarly journals Depletion of CD8αβ+ T Cells in Chickens Demonstrates Their Involvement in Protective Immunity towards Marek’s Disease with Respect to Tumor Incidence and Vaccinal Protection

Vaccines ◽  
2020 ◽  
Vol 8 (4) ◽  
pp. 557
Author(s):  
Supawadee Umthong ◽  
John R. Dunn ◽  
Hans H. Cheng
Keyword(s):  
T Cells ◽  
Cd8 T Cells ◽  
Protective Immunity ◽  
Lymphoproliferative Disease ◽  
Tumor Formation ◽  
Marek's Disease ◽  
Control Measures ◽  
Marek’S Disease ◽  
Cell Mediated Immune Response

Marek’s disease (MD) is a lymphoproliferative disease in chickens caused by Marek’s disease virus (MDV), a highly oncogenic alphaherpesvirus. Since 1970, MD has been controlled through widespread vaccination of commercial flocks. However, repeated and unpredictable MD outbreaks continue to occur in vaccinated flocks, indicating the need for a better understanding of MDV pathogenesis to guide improved or alternative control measures. As MDV is an intracellular pathogen that infects and transforms CD4+ T cells, the host cell-mediated immune response is considered to be vital for controlling MDV replication and tumor formation. In this study, we addressed the role of CD8+ T cells in vaccinal protection by widely-used monovalent (SB-1 and HVT) and bivalent (SB-1+HVT) MD vaccines. We established a method to deplete CD8+ T cells in chickens and found that their depletion through injection of anti-CD8 monoclonal antibodies (mAb) increased tumor induction and MD pathology, and reduced vaccinal protection to MD, which supports the important role of CD8+ T cells for both MD and vaccinal protection.

scholarly journals Prevalence of Chromosomal Abnormalities in Infertile Couples in Romania

2015 ◽  
Vol 18 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Dana Mierla ◽  
M. Malageanu ◽  
R. Tulin ◽  
D. Albu
Keyword(s):  
Retrospective Study ◽  
Chromosomal Abnormalities ◽  
Normal Karyotype ◽  
Control Group ◽  
Chromosomal Anomalies ◽  
Exact Test ◽  
Infertile Women ◽  
Infertile Men ◽  
Significant Statistical Association

AbstractThe purpose of this study was to establish a correlation between the presence of chromosomal abnormalities in one of the partners and infertility. This retrospective study was performed at the Department of Reproductive Medicine, Life Memorial Hospital, Bucharest, Romania, between August 2007 to December 2011. Two thousand, one hundred and ninety-five patients with reproductive problems were investigated, and the frequency of chromosomal abnormalities was calculated. The control group consisting of 87 fertile persons who had two or more children, was investigated in this retrospective study. All the patients of this study were investigated by cytogenetic techniques and the results of the two groups were compared by a two-tailed Fisher’s exact test. In this study, 94.99% patients had a normal karyotype and 5.01% had chromosomal abnormalities (numerical and structural chromosomal abnormalities). In the study group, numerical chromosomal abnormalities were detected in 1.14% of infertile men and 0.62% of infertile women, and structural chromosomal abnormalities were detected in 1.38% of infertile men and 1.87% of infertile women, respectively. The correlation between the incidence of chromosomal anomalies in the two sexes in couple with reproductive problems was not statistically significant. Recently, a possible association between infertility and chromosomal abnormalities with a significant statistical association has been reported. Our study shows that there is no association between chromosomal abnormalities and infertility, but this study needs to be confirmed with further investigations and a larger control group to establish the role of chromosomal abnormalities in the etiology of infertility.

scholarly journals Effects of Titanium Surfaces on the Developmental Profile of Monocytes/Macrophages

2014 ◽  
Vol 25 (2) ◽  
pp. 96-103 ◽  
Author(s):  
Camilla Christian Gomes Moura ◽  
Darceny Zanetta-Barbosa ◽  
Paula Dechichi ◽  
Valessa Florindo Carvalho ◽  
Priscilla Barbosa Ferreira Soares
Keyword(s):  
Cell Viability ◽  
Colorimetric Assay ◽  
Initial Period ◽  
Critical Role ◽  
Surface Characteristics ◽  
Th2 Cytokine ◽  
Titanium Surfaces ◽  
Control Surfaces ◽  
Immunoenzymatic Assays

Due to the critical role of monocytes/macrophages (Mϕ) in bone healing, this study evaluated the effects of bio-anodized, acid-etched, and machined titanium surfaces (Ti) on Mϕ behavior. Cells were separated from whole human blood from 10 patients, plated on Ti or polystyrene (control) surfaces, and cultured for 72 h. At 24, 48 and 72 h, cell viability, levels of IL1β, IL10, TNFα, TGFβ1 inflammatory mediators, and nitric oxide (NO) release were analyzed by mitochondrial colorimetric assay (MTT assay) and immunoenzymatic assays, respectively. Real-time PCR was used to verify the expression of TNFα and IL10 at 72 h. The data were subjected to a Kruskal-Wallis analysis. IL1β, TNFα and TGFβ1 release were not significantly different between the Ti surfaces (p>0.05). The presence of NO and IL10 was not detected in the samples. Cell viability did not differ between the samples cultivated on Ti and those cultivated on control surfaces, except at 24 h (p=0.0033). With respect to the mediators evaluated, the surface characteristics did not induce a typical Th1 or Th2 cytokine profile, although the cell morphology and topography were influenced by the Ti surface during the initial period.

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