Accelerated aging test and its relationship to physiological potential of soybean seeds

Abstract: Although accelerated aging is one of the tests most used for evaluation of the physiological quality of soybean seeds, there are few studies that relate it to other physiological tests used for the crop. In this context, the objective of this study was to investigate the relationship of accelerated aging to the results of physiological performance obtained in the vigor tests most frequently used in the soybean quality control system (germination, first count, seedling vigor classification, accelerated aging, electrical conductivity, emergence speed index, final emergence in sand substrate, and field emergence). The experiment was conducted with seeds that had different vigor levels, adopting a completely randomized experimental design with four replications. Pearson simple correlation analysis (r) and simple linear regression analysis at both 1% and 5% probability were conducted on the data. The results showed that the seedling vigor classification test (r = 0.9160) and the field emergence test (r = 0.9198) were those that best correlated with accelerated aging, followed by the germination (r = 0.8690), first count of germination (r = 0.8460), and electrical conductivity (r = 0.8912) tests.

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Physiological potential of soybean seeds and its relationshipto electrical conductivity

Journal of Seed Science ◽

10.1590/2317-1545v41n4214988 ◽

2019 ◽

Vol 41 (4) ◽

pp. 407-415

Author(s):

Jéssica Pavão do Prado ◽

Francisco Carlos Krzyzanowski ◽

Cibele Chalita Martins ◽

Roberval Daiton Vieira

Keyword(s):

Electrical Conductivity ◽

Regression Analysis ◽

Linear Regression Analysis ◽

Accelerated Aging ◽

Soybean Seeds ◽

Seedling Vigor ◽

Performance Potential ◽

Initial Stage ◽

Range Of Values ◽

Very High

Abstract: Electrical conductivity (EC) stands out among vigor tests because of its capability of detecting damage in seeds in the initial stage, since EC is related to disorganization of the cell membrane. The aim of this paper was to study use of the EC test as an alternative for vigor evaluation in soybean seeds, verifying its relationship to different vigor tests and thus suggesting values and the range of values of electrical conductivity that indicate the performance potential of a seed lot in the field. Eleven seed lots from each of four soybean cultivars were used for this study. The moisture level was determined and the following tests were performed: electrical conductivity, germination, tetrazolium, accelerated aging, first germination count, seedling vigor classification and emergence of seedlings in the field and in sand. The EC test had a significant and negative correlation (p < 0.01) with all the tests performed. Linear regression analysis allowed separation of the seed lots into classifications of very high vigor (EC ≤ 70 μS.cm-1.g-1), high vigor (EC from 71 to 90 μS.cm-1.g-1), medium vigor (EC from 91 to 110 μS.cm-1.g-1) and low vigor (EC ≥ 111 μS.cm-1.g-1).

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Pre-packing cooling and types of packages in maintaining physiological quality of soybean seeds during storage

Journal of Seed Science ◽

10.1590/2317-1545v38n2158956 ◽

2016 ◽

Vol 38 (2) ◽

pp. 129-139 ◽

Cited By ~ 4

Author(s):

Everson Reis Carvalho ◽

João Almir Oliveira ◽

Denilson Paulo da Rosa Mavaieie ◽

Hellismar Wakson da Silva ◽

Cassiano Gabriel Moreira Lopes

Keyword(s):

Electrical Conductivity ◽

Experimental Design ◽

Seed Mass ◽

Accelerated Aging ◽

Soybean Seeds ◽

Randomized Experimental Design ◽

Deterioration Process ◽

Physiological Quality ◽

Polyethylene Container

Abstract: Seeds storing under appropriate conditions reduces the speed of the seeds deterioration process. The aim of this study was to evaluate pre-packing cooling and types of packages in the maintenance of the physiological quality of soybean seeds during storage. The completely randomized experimental design was used, with four replications, considering a factorial arrangement involving or not the pre-packing cooling of the seed mass, three types of packages (multi-layer paper bag, big bag, and polyethylene container), and five storing periods (0, 2, 4, 6 and 8 months). The physiological quality was evaluated by the tests of first germination count, germination, accelerated aging, electrical conductivity, emergence speed index and emergence. The use of packages with larger amount of seeds (big bag and polyethylene container) did not favor the maintenance of the pre-packing cooling temperature. The types of packages did not show significant differences in the seeds viability during storage, up to eight months, regardless the pre-packing cooling. The cultivars seeds showed different tolerance to storing, i.e. seeds of the cultivar TMG 1176 had lower storing potential comparing to the seeds from the cultivar SYN 9074.

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SUITABILITY OF ELECTRICAL CONDUCTIVITY TESTING AND ACCELERATED AGING FOR EVALUATING THE VIGOR OFCITHAREXYLUM MYRIANTHUM CHAM. DIASPORES

FLORESTA ◽

10.5380/rf.v49i4.57765 ◽

2019 ◽

Vol 49 (4) ◽

pp. 701

Author(s):

Ana Paula Bazzanella ◽

Gabriele Larissa Mathias ◽

Henrique Roberto Gattermann Mittelstaedt ◽

Maiara Iadwizak Ribeiro ◽

Andréa Maria Teixeira Forte ◽

...

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Germination Test ◽

High Demand ◽

Tukey Test ◽

Aging Period ◽

Accelerated Aging Test ◽

Aging Test ◽

Randomized Experimental Design ◽

Native Tree Species

The Atlantic Forest is one of the most threatened Brazilian biomes by anthropic action. Thus, the interest inrecovering degraded areas through the use of native tree species has increased, causing a high demand in the use of vigorous seeds. This research aimed at evaluating the suitabilityof the electrical conductivity and accelerated aging tests to evaluate the vigor of C. myrianthum diaspores. For the adequacy of the electrical conductivity test, a completely randomized experimental design (CRD) was used in a 2 x 3 x9 (number of diaspores x volume of water x imbibition time)at a constant temperature of 25 °C. The procedure for the accelerated aging test was conducted at 40 ºC for 0 (no aging), 24, 48 and 72 hours. ACRDwas also used for the accelerated aging test, totaling 4 treatments. The results were submitted to analysis of variance (ANOVA) and the means were compared by the Tukey test at 5% probability. It is concluded that the electrical conductivity test for C. myrianthum diaspores can be conducted with 25 diaspores soaked with 50 mL of deionized water for 96 hours, thus replacing the germination test with the advantage of being carried out in a shorter period of time. Moreover,the 24 and 48 hour accelerated aging period is adequate to discriminate the vigor of C. myrianthum diaspores, with GSI and MGT being the most sensitive variables.

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Hydric restriction as vigor assessment method of soybean seeds

Revista Brasileira de Sementes ◽

10.1590/s0101-31222012000200008 ◽

2012 ◽

Vol 34 (2) ◽

pp. 241-246 ◽

Cited By ~ 2

Author(s):

Denis Santiago da Costa ◽

Cartiane Rubishirley Macêdo da Rocha ◽

Ana Dionisia da Luz Coelho Novembre

Keyword(s):

Aqueous Solution ◽

Polyethylene Glycol ◽

Water Potential ◽

Accelerated Aging ◽

Assessment Method ◽

Deionized Water ◽

Soybean Seeds ◽

Accelerated Aging Test ◽

Aging Test ◽

Randomized Experimental Design

The objective of this study was to investigate the possibility of using hydric restriction as a method for evaluating vigor of soybean seeds. The soybean seeds, cultivar BRS 245RR, represented by four different seed lots, were characterized by germination and vigor. For the treatment of hydric restriction and temperature, the combination of substrate water potential and temperature were the following: deionized water (0.0 MPa); polyethylene glycol (PEG 6000) aqueous solution (-0.1, -0.3 and -0.5 MPa); and four temperatures (20 ºC, 25 ºC, 30 ºC, and 35 ºC), respectively. A completely randomized experimental design was used, with four replications per treatment, and the ANOVA was performed individually for each combination of temperature and water potential of substrate. According to results obtained, the test of hydric restriction has the same efficiency of the accelerated aging test in estimating vigor of soybean seeds, cv. BRS 245RR, when water potentials of -0.1 MPa or -0.3 MPa at a temperature of 25 ºC, or -0.3 MPa at a temperature of 30 ºC are used.

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Electrical conductivity and accelerated aging in amaranth (Amaranthus crueentus L.) seeds

Journal of Seed Science ◽

10.1590/2317-1545v40n1183116 ◽

2018 ◽

Vol 40 (1) ◽

pp. 44-51

Author(s):

Thaís D’Avila Rosa ◽

Ariele Paula Nadal ◽

Henrique Roberto Maldaner ◽

Vanessa Nogueira Soares ◽

Gizele Ingrid Gadotti ◽

...

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Water Volume ◽

Amaranthus Cruentus ◽

Accelerated Aging Test ◽

Aging Test ◽

Immersion Period ◽

Amaranth Seed

Abstract: The objective of this study was to determine adequate conditions of water volume, seed quantity and immersion period to perform the electrical conductivity test, and to analyze the parameters of the accelerated aging test and its correlation with the the physiological potential of amaranth seeds. Five lots of amaranth seeds (Amaranthus cruentus), cultivar BRS Alegria, were used. The initial evaluation of the quality of these lots comprehended the following tests: water content, germination, first germination count, accelerated aging (traditional method, and modified versions, using unsaturated and saturated saline solution for the periods of 24, 48 and 72 h), electrical conductivity in different periods (2, 4, 6, 8 and 24 h), different quantities of water (25, 50 and 75 mL), different seed quantities (100 and 150), and field emergence. It could be concluded that the accelerated aging test at 41 °C, for 72 h, using unsaturated NaCl solution was efficient in the classification of amaranth seed lots in levels of vigor. The electrical conductivity test performed for 8 hours with 25 mL of water and 100 seeds was efficient in the classification of amaranth seeds as to their physiological potential.

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PRODUCTION AND PHYSIOLOGICAL QUALITY OF SOYBEAN SEEDS IN ORTHIC QUARTZARENIC NEOSOIL OF THE CERRADO REGION

Revista Caatinga ◽

10.1590/1983-21252015v28n414rc ◽

2015 ◽

Vol 28 (4) ◽

pp. 127-134

Author(s):

CHARLINE ZARATIN ALVES ◽

GUSTAVO MARTINS ZAQUEU ◽

ERIC FABIANO SERAGUZI ◽

AGUINALDO JOSÉ FREITAS LEAL ◽

JOSUÉ BISPO DA SILVA

Keyword(s):

Electrical Conductivity ◽

Aerial Part ◽

Water Distribution ◽

Accelerated Aging ◽

Dry Mass ◽

Soybean Seeds ◽

Scheme Design ◽

Physiological Quality ◽

Tetrazolium Test

ABSTRACT: The aim of this study was to evaluate the effect of the sowing periods in production, productivity component and physiological quality of seeds of three soybean cultivars (TMG133RR, P98Y70RR and NS7670RR) in Orthic Quartzarenic Neosoil in the cerrado region. The design used was a randomized block a factorial scheme design with four repetitions, and each plot with useful space consisted of three rows of four meters length, spaced at 0.45 meters. Field evaluations were the final stand, the height of the plants, height of the first pod insertion, the mass of 100 seeds and productivity. Already in the lab seeds were evaluated for germination and vigor (first germination count, emergency, emergence speed index, length and dry mass of the aerial part of the plant and roots, electrical conductivity, accelerated aging and tetrazolium test). It concludes that it is possible to use Orthic Quartzarenic Neosoils, located in the Cerrado region at altitudes higher than 600 m, in years and places with good water distribution in the spring-summer seasons, for grain and soybeans production, but as a first goal, the crop should be sown in the first 20 days of November otherwise it should take place in early December.

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Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?

Journal of Seed Science ◽

10.1590/2317-1545v43249697 ◽

2021 ◽

Vol 43 ◽

Author(s):

Thiago Barbosa Batista ◽

Samara Moreira Perissato ◽

Carlos Henrique Queiroz Rego ◽

Gustavo Roberto Fonseca de Oliveira ◽

Fernando Augusto Henning ◽

...

Keyword(s):

Electrical Conductivity ◽

Linear Regression ◽

Soybean Seed ◽

Accelerated Aging ◽

Storage Period ◽

High Potential ◽

Seed Longevity ◽

Soybean Seeds ◽

Radicle Protrusion ◽

Over Time

Abstract: The analysis of longevity can support decisions about the length of seed lot storage until commercialization, since this characteristic implies the maintenance of viability over time. Seed longevity is analyzed by the p50 test, which expresses the time to lose 50% of the initial viability. Seeds with high vigor and germination have greater physiological potential and, thus, a greater capacity to maintain quality throughout the storage period. However, there has been little research on the correlations between the analysis of p50 (longevity) and the tests used to measure physiological potential (germination and vigor) of lots, which can be used as a tool to make inferences about longevity using the most traditional tests. Thus, the objective of this study was to investigate which tests used to measure the potential of lots can estimate p50. To this end, germination and vigor were evaluated using traditional tests while longevity was assessed in eight soybean seed lots. Correlations and linear regression were tested for the traditional variables versus p50. It was found that the use of accelerated aging, electrical conductivity, and time to 50% radicle protrusion has high potential to estimate longevity as measured by p50.

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Presence of glyphosate can harm the germination of bean seeds treated with biostimulant

Bioscience Journal ◽

10.14393/bj-v36n1a2020-42441 ◽

2020 ◽

Vol 36 (1) ◽

Author(s):

Jorgiani de Ávila ◽

Fernanda Brito Cardoso ◽

Sebastião Ferreira de Lima ◽

Gustavo Ribeiro Barzotto ◽

Mayara Santana Zanella

Keyword(s):

Electrical Conductivity ◽

Accelerated Aging ◽

Cold Test ◽

Seed Vigor ◽

Common Beans ◽

Accelerated Aging Test ◽

Root And Shoot Length ◽

Aging Test ◽

Early Seedling ◽

Different Doses

It is possible to cultivate common beans for a third harvest in one agricultural year due to varietal characteristics. For calendar adequacy, performing desiccation and planting often occur almost simultaneously. Germination performance of many plant species can improve with biostimulant use on seeds, however the interaction with herbicide residual molecules is unknown. The hypothesis is that seeds treated with a biostimulant in soil with glyphosate residues can eliminate the advantage of the biostimulant or increase the damage caused by the herbicide. The aim of this study was to evaluate the effect of different glyphosate reduce doses and the interaction with biostimulant on bean seed germination and vigor. The experiment was conducted using a completely randomized 2 x 5 factorial block designing factorial 2 x 5, corresponding to the presence and absence of biostimulant and five different doses of glyphosate reduce rates, with four repetitions. The conducted evaluations were first count germination, germination test, accelerated aging, cold test, root and shoot length, root and shoot dry matter and electrical conductivity. It can be concluded that the biostimulant treatment on bean seeds increased germination, seed vigor and early seedling growth, but glyphosate presence reduced those advantages, increasing electrical conductivity. However, the herbicide presence provided higher germination on the accelerated aging test.

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Reduction of the Forage Seeds Physiological Quality by the Contact with Fertilizer in the Crop-Livestock Integration

Journal of Experimental Agriculture International ◽

10.9734/jeai/2019/v34i630190 ◽

2019 ◽

pp. 1-11

Author(s):

Anne Caroline Dallabrida Avelino ◽

Dayana Aparecida De Faria ◽

Carlos Eduardo Avelino Cabral ◽

Joadil Gonçalves De Abreu ◽

Elisangela Clarete Camili ◽

...

Keyword(s):

Electrical Conductivity ◽

Contact Time ◽

Accelerated Aging ◽

Germination Percentage ◽

Randomized Design ◽

Accelerated Aging Test ◽

Aging Test ◽

Physiological Quality ◽

Completely Randomized Design ◽

Seeds Germination

The aim of this study was to evaluate the effects of Brachiaria seed contact time with 05-25-15 fertilizer (N-P2O5-K2O) on the seed’s physiological quality. The experimental design was a completely randomized design with four replicates. Treatments were arranged in a 5x4 factorial, being: five seeds contact times with fertilizer (0, 24, 48, 72 and 96 hours); four forages: Marandu palisadegrass, Piatã palisadegrass, Xaraés palisadegrass and Ruziziensis grass. The performed tests were: water content, germination (first count and germination percentage), tetrazolium (viability), electrical conductivity, accelerated aging, sand emergency and emergence speed index. Contact time of 05-25-15 fertilizer with Brachiaria seeds reduces linearly: Germination, emergence and emergence speed index of Brachiaria seeds; Piatã and Xaraés palisadegrass seeds viability; vigor (electrical conductivity), except in Ruziziensis grass seeds; and seeds germination of Piatã palisadegrass submitted to accelerated aging test (vigor). Piatã palisadegrass is the least recommended, among the analyzed grasses, for intercropping in simultaneous sowing.

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Accelerated Aging Test to Determine the Vigor of Mungbean Seeds

Journal of Agricultural Science ◽

10.5539/jas.v11n7p42 ◽

2019 ◽

Vol 11 (7) ◽

pp. 42

Author(s):

Luciana Maria da Silva ◽

Anderson Dias Vaz de Souza ◽

Rogério Lámim Silva Junior ◽

Natália Arruda ◽

Nei Peixoto ◽

...

Keyword(s):

Seed Quality ◽

Germination Rate ◽

Accelerated Aging ◽

Dry Weight ◽

Precise Determination ◽

Germination Test ◽

Accelerated Aging Test ◽

Seedling Length ◽

Aging Test ◽

Randomized Experimental Design

The use of good quality seeds is fundamental for proper establishment of a crop. In this way, for more precise determination of seed quality, vigor tests are performed in addition to the germination test. These tests enable the selection of the best lots for commercialization and planting. This study evaluates the effects of temperature and exposure times of the accelerated aging test for vigor classification of mungbean seed lots. Seeds of the mungbean cultivar Esmeralda were used, being obtained from four cultivated plots at the State University of Goiás (UEG), Ipameri Câmpus, in the 2013, 2015, 2016, and 2017 harvests. The lots were initially characterized using germination and vigor tests (first germination count, germination rate index, seedling length, and seedling fresh and dry weight). The accelerated aging test was conducted with a completely randomized experimental design, in a 2 × 4 factorial arrangement consisting of two temperatures (40 and 42 °C) and four times (24, 48, 72, and 96 hours), with four replicates of 50 seeds per lot. The lots showed significant differences in the germination test, first germination count, germination rate index, and seedling length. The accelerated aging test was efficient in classifying lots. Lot 3 obtained the best results, while lot 1 obtained the lowest ones. The combination of 42 °C temperature and 72 h of seed exposure to the accelerated aging test is the best to classify mungbean seed lots.

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