Computerized analysis of seedling performance in evaluating the phytotoxicity of chemical treatment of soybean seeds

Abstract: Chemical treatment of soybean seeds is very important to ensure successful crop establishment. However, problems such as phytotoxicity of product combinations that can reduce seed physiological performance require attention. The use of computational resources has shown potential in identifying phytotoxic effects and contributing to the steps of quality control of treated seeds. The aim of this study was to determine if computerized image analysis of seedlings enables the phytotoxicity of chemical treatment of soybean seeds to be assessed in an effective and simplified manner. Samples from two soybean seed lots were treated with fungicides, insecticides, micronutrients, and their combinations, as well as with polymer and drying powder (coatings). After chemical treatment, the seeds were evaluated for germination, first germination count, seedling emergence in sand, accelerated aging, and seedling performance with and without the correction of regions not automatically demarcated (Vigor-S). We found high correlation of the Vigor-S parameters with the traditional tests for detection of phytotoxic effects of chemical treatment, regardless of correction made in the system. Computerized image analysis of seedlings is an effective and highly sensitive resource for evaluating possible phytotoxicity effects due to chemical treatment of soybean seeds.

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Soybean Industrial Seed Treatment: Effect on Physiological Quality During Storage

Journal of Agricultural Science ◽

10.5539/jas.v10n8p468 ◽

2018 ◽

Vol 10 (8) ◽

pp. 468

Author(s):

C. R. Bork ◽

A. S. Almeida ◽

C. S. Castellano ◽

G. Zimmer ◽

T. D. Avila ◽

...

Keyword(s):

Environmental Conditions ◽

Seed Treatment ◽

Seed Quality ◽

Seedling Emergence ◽

Soybean Seed ◽

Accelerated Aging ◽

Soybean Seeds ◽

Distilled Water ◽

Physiological Quality ◽

Spray Volume

The aim of this study was to analyze soybean seed physiological quality after being subjected to various mixtures of pesticides via industrial seed treatment. The experiment was performed at the seed laboratory of the company BioGrow, located at São Paulo-SP, using soybean seeds cultivar NS 6700 IPRO which were subjected to 11 different treatments. Seed treatment was carried out using a treater Momesso, model L5-K, calibrated to apply a spray volume of 0.5 L 100 kg-1 of seeds in which the volume of each treatment was adjusted using distilled water. After treatment, seeds were spread over plastic strays for drying for a period of 24 hours under environmental conditions. Once dry, seeds were packed in paper bags and stored for 0 (control), 45, 90, 135 and 180 days, under uncontrolled conditions of temperature and relative humidity, when seed physiological quality was evaluated using the following tests: germination, accelerated aging, seedling emergence, speed of emergence index and speed of emergence. Soybean industrial seed treatment before storage for up to 180 days is practicable using the mixtures of pesticides tested for storing seeds under environmental conditions. All treatments tested contribute to the maintenance of seed quality throughout storage.

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Incidence of storage fungi and hydropriming on soybean seeds

Journal of Seed Science ◽

10.1590/s2317-15372013000100005 ◽

2013 ◽

Vol 35 (1) ◽

pp. 35-41 ◽

Cited By ~ 3

Author(s):

Denis Santiago da Costa ◽

Nathalie Bonassa ◽

Ana Dionisia da Luz Coelho Novembre

Keyword(s):

Seed Germination ◽

Moisture Content ◽

Seedling Emergence ◽

Mechanical Damage ◽

Soybean Seed ◽

Accelerated Aging ◽

Soybean Seeds ◽

Storage Fungi ◽

Randomized Experimental Design ◽

Low Incidence

Priming is a technique applicable to seeds of various plant species; however, for soybean seed there is little available information correlating such technique to the storage fungi. The objective of this study was to assess hydropriming on soybeans seeds and correlate this technique to occurrence of such fungi. For this, soon after acquisition the soybean seeds, cv. M-SOY 7908 RR, were characterized by: moisture content, mechanical damage, viability (seed germination and seedling emergence) and seed health. A completely randomized experimental design was used with treatments arranged into a factorial scheme 2 × 2 [2 levels of incidence of storage fungi (low and high) × 2 hydropriming (with and without) ]. After application of treatments, the seeds were analyzed by: moisture content, viability (seed germination and seedling emergence) and vigor (first count and accelerated aging). The hydropriming is beneficial to improve the quality of soybean seeds with low incidence of storage fungi, with increments on speed germination (first count) and seed germination after accelerated aging test. The high incidence of microorganisms can reduce the hydropriming benefits.

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Impact of storage on the physiological quality of soybean seeds after treatment with fungicides and insecticides

Journal of Seed Science ◽

10.1590/2317-1545v42236236 ◽

2020 ◽

Vol 42 ◽

Author(s):

Luciano Del Bem Junior ◽

Jonas Leandro Ferrari ◽

Gustavo Dario ◽

Carlos Gilberto Raetano

Keyword(s):

Chemical Treatment ◽

Seedling Emergence ◽

Maximum Yield ◽

Accelerated Aging ◽

Storage Period ◽

Yield Potential ◽

Soybean Seeds ◽

Thiophanate Methyl ◽

Physiological Quality

Abstract: Chemical seed treatment is a practice that assists in control of pests and pathogens and allows crops to achieve their maximum yield potential. However, the storage period of seeds treated with insecticides and fungicides can affect seed physiological potential. The aim of this study was to evaluate the physiological quality of soybean seeds treated with different fungicides and insecticides after four different storage periods. The experimental design was completely randomized and the treatments were distributed in a 10 × 4 factorial arrangement in which the soybean seeds of the cultivar BMX Potência RR were treated with the following phytosanitary products: fipronil + pyraclostrobin + thiophanate-methyl, thiophanate-methyl, carbendazim, carboxin + thiram, imidacloprid + thiodicarb, chlorantraniliprole, thiamethoxam, cyantraniliprole, fipronil, and a control (seeds without chemical treatment), in four replications. The seeds were evaluated at 0, 30, 60, and 90 days after the chemical treatment. The following tests were performed: germination, first count of germination, accelerated aging, seedling emergence in sand, seedling emergence speed, and seedling dry matter. The treatment with the carboxin + thiram fungicide mixture provided for adequate physiological quality of the seeds throughout storage. The insecticide thiamethoxam has a negative effect on the physiological quality of soybean seeds that are stored after treatment with it.

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Computerized image analysis of seedlings to evaluate broccoli seed vigor

Journal of Seed Science ◽

10.1590/2317-1545v39n3174582 ◽

2017 ◽

Vol 39 (3) ◽

pp. 303-310 ◽

Cited By ~ 4

Author(s):

Haynna Fernandes Abud ◽

Silvio Moure Cicero ◽

Francisco Guilhien Gomes Junior

Keyword(s):

Image Analysis ◽

Imaging System ◽

Seedling Emergence ◽

Accelerated Aging ◽

Seed Vigor ◽

Computer Image Analysis ◽

Computerized Image Analysis ◽

Randomized Design ◽

Completely Randomized Design

Abstract: The computerized image analysis of seedlings constitutes an efficient technique to evaluate the quality of seed lots, since it is quick in responding, has a simple execution and reproduction. The goal of this research was to verify the efficiency of the computer image analysis of seedlings to detect vigor differences among broccoli seed lots and compare them with other traditionally used vigor tests. Computerized image analysis were performed by the Seed Vigor Imaging System (SVIS®), using 3-day-old seedlings and the results were compared to germination at first count, seedling emergence, accelerated aging and electrical conductivity tests. Evaluations were performed in three experimental periods (before storage, 6 and 12 months of storage at 20 °C and 45-50% RH). A completely randomized design with eight replications of 25 seeds was used. Data were submitted to analysis of variance and means were compared by Scott-Knott test at 5% error probability. It is concluded that it is possible to detect vigor differences among broccoli seed lots by the computerized image analysis of seedlings with SVIS®, similarly to traditional vigor tests.

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Vigor-S: System for Automated Analysis of Soybean Seed Vigor

Journal of Seed Science ◽

10.1590/2317-1545v42237490 ◽

2020 ◽

Vol 42 ◽

Author(s):

Mayara Rodrigues ◽

Francisco G. Gomes-Junior ◽

Julio Marcos-Filho

Keyword(s):

Imaging System ◽

Seedling Emergence ◽

Soybean Seed ◽

Accelerated Aging ◽

Field Tests ◽

Automated Analysis ◽

Seed Vigor ◽

Soybean Seeds ◽

Mean Values ◽

Randomized Experimental Design

Abstract: Computerized systems for image analysis are alternatives to decrease the subjectivity and speed of assessment of seed physiological potential. The aim of this study was to determine the efficiency of the Seed Vigor Automated Analysis System (Vigor-S) to identify differences in vigor among soybean seed lots compared to results of the Seed Vigor Imaging System - SVIS® and tests recommended for evaluation of soybean seed vigor. Two cultivars were used, BMX Potência RR and 7166 RSF IPRO, each one represented by ten seed lots with similar germination and vigor differences. The seeds were evaluated regarding germination, vigor (tetrazolium, accelerated aging), seedling emergence in the field and SVIS® and Vigor-S analysis, in three experimental periods. Analysis of variance was used on the data in a completely randomized experimental design (laboratory tests) and in randomized blocks (field tests), and the mean values were compared by the Tukey test (p ≤ 0.05). Separation of the seed lots by Vigor-S was consistent with the results obtained in tests recognized as effective in evaluating the physiological potential of soybean seeds, and there was no interference of the different cultivars on the response pattern for vigor. Analysis through Vigor-S proved to be effective in determination of physiological potential and for composition of quality control programs established by soybean seeds production companies.

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Physiological potential of soybean seeds after maturation and submitted to artificial drying

Journal of Seed Science ◽

10.1590/2317-1545v39n4175656 ◽

2017 ◽

Vol 39 (4) ◽

pp. 374-384

Author(s):

Cesar Pedro Hartmann Filho ◽

André Luís Duarte Goneli ◽

Tathiana Elisa Masetto ◽

Elton Aparecido Siqueira Martins ◽

Guilherme Cardoso Oba

Keyword(s):

Moisture Content ◽

Dry Matter ◽

Seedling Emergence ◽

Accelerated Aging ◽

Maturation Stage ◽

Soybean Seeds ◽

Germination Test ◽

Moisture Contents ◽

Three Samples ◽

Maturation Stages

Abstract: This study evaluated the physiological potential of soybean seeds harvested during two seasons, on different maturation stages and subjected to different drying temperatures. The seeds were harvested at the maturations stages R7, R7 + 2, R7 + 3, R7 + 5, R7 + 6, R7 + 7, R7 + 10 and R7 + 12 days (55, 50, 45, 40, 35, 30, 25, and 20% of moisture content). For each maturation stage, seeds were divided into three samples: one sample was used to directly evaluate the physiological potential, and the others were dried at 40 °C and 50 °C, until reaching the moisture content of 11.5%. The physiological potential was evaluated through germination test, first germination count of germination, accelerated aging, modified cold, electrical conductivity and seedling emergence. The maximum physiological potential of seeds is achieved at the moisture content of 55%, the point that the dry matter is maximum. The seeds became tolerant to artificial drying approximately at the stage R7 + 7 days (30% of moisture content). Germination and vigor of the soybean seeds reduce as the drying temperature is increased from 40 °C to 50 °C, and this effect is enhanced when the seeds show moisture contents above 30%.

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Electrical conductivity of the seed soaking solution and soybean seedling emergence

Scientia Agricola ◽

10.1590/s0103-90162004000200007 ◽

2004 ◽

Vol 61 (2) ◽

pp. 164-168 ◽

Cited By ~ 27

Author(s):

Roberval Daiton Vieira ◽

Angelo Scappa Neto ◽

Sonia Regina Mudrovitsch de Bittencourt ◽

Maristela Panobianco

Keyword(s):

Electrical Conductivity ◽

Water Content ◽

Seedling Emergence ◽

Soybean Seed ◽

Accelerated Aging ◽

Seed Vigor ◽

Soybean Seedling ◽

Seed Soaking ◽

Laboratory Conditions ◽

Field And Laboratory Conditions

Vigor of soybean [Glycine max (L.) Merrill] seeds can be evaluated by measuring the electrical conductivity (EC) of the seed soaking solution, which has shown a satisfactory relationship with field seedling emergence, but has not had aproper definition of range yet. This work studies the relationship between EC and soybean seedling emergence both in the field and laboratory conditions, using twenty two seed lots. Seed water content, standard germination and vigor (EC, accelerated aging and cold tests) were evaluated under laboratory conditions using -0.03; -0.20; -0.40 and -0.60 MPa matric potentials, and field seedling emergence was also observed. There was direct relationship between EC and field seedling emergence (FE). Under laboratory conditions, a decreasing relationship was found between EC and FE as water content in the substrate decreased. Relationships between these two parameters were also found when -0.03; -0.20 and -0.40 MPa matric potentials were used. EC tests can be used successfully to evaluate soybean seed vigor and identify lots with higher or lower field emergence potential.

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Is it possible to estimate longevity through the analyses used to measure the initial physiological potential in soybean seeds?

Journal of Seed Science ◽

10.1590/2317-1545v43249697 ◽

2021 ◽

Vol 43 ◽

Author(s):

Thiago Barbosa Batista ◽

Samara Moreira Perissato ◽

Carlos Henrique Queiroz Rego ◽

Gustavo Roberto Fonseca de Oliveira ◽

Fernando Augusto Henning ◽

...

Keyword(s):

Electrical Conductivity ◽

Linear Regression ◽

Soybean Seed ◽

Accelerated Aging ◽

Storage Period ◽

High Potential ◽

Seed Longevity ◽

Soybean Seeds ◽

Radicle Protrusion ◽

Over Time

Abstract: The analysis of longevity can support decisions about the length of seed lot storage until commercialization, since this characteristic implies the maintenance of viability over time. Seed longevity is analyzed by the p50 test, which expresses the time to lose 50% of the initial viability. Seeds with high vigor and germination have greater physiological potential and, thus, a greater capacity to maintain quality throughout the storage period. However, there has been little research on the correlations between the analysis of p50 (longevity) and the tests used to measure physiological potential (germination and vigor) of lots, which can be used as a tool to make inferences about longevity using the most traditional tests. Thus, the objective of this study was to investigate which tests used to measure the potential of lots can estimate p50. To this end, germination and vigor were evaluated using traditional tests while longevity was assessed in eight soybean seed lots. Correlations and linear regression were tested for the traditional variables versus p50. It was found that the use of accelerated aging, electrical conductivity, and time to 50% radicle protrusion has high potential to estimate longevity as measured by p50.

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Physiological quality of dynamically cooled and stored soybean seeds

Journal of Seed Science ◽

10.1590/s2317-15372013000300012 ◽

2013 ◽

Vol 35 (3) ◽

pp. 353-360 ◽

Cited By ~ 16

Author(s):

Jacson Zuchi ◽

José de Barros França-Neto ◽

Carlos Sigueyuki Sediyama ◽

Adílio Flauzino de Lacerda Filho ◽

Múcio Silva Reis

Keyword(s):

Dynamic Process ◽

Seed Quality ◽

Seedling Emergence ◽

Accelerated Aging ◽

Superior Performance ◽

Soybean Seeds ◽

Tropical Regions ◽

Physiological Quality ◽

Feasible Alternative

Storage is an important step in the production of soybean seeds, mainly in tropical regions, where high temperatures can drastically reduce seed quality. The storage at cooler temperatures may be a feasible alternative to preserve seed quality during storage. The objective of this study was to evaluate the physiological quality of soybean seeds artificially cooled by a dynamic process and stored in an environment, with non controlled temperature and relativity humidity. Two seed lots of cultivars Monsoy 8757, TMG 115 RR and BRS Valiosa RR were used. After processing and during packaging the seeds were cooled to 18 ºC by a dynamic process. Seed samples were collected after 0, 60 and 120 days of storage, using the following parameters germination, accelerated aging, seedling emergence in sand, speed of emergence index, electrical conductivity, tetrazolium and seed health. Although soybean seeds artificially cooled by the dynamic method may have slightly superior performance in maintaining physiological quality during storage in non-refrigerated warehouse, for lots of high physiological quality, these advantages are not observed.

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Spatial variability in soybean seeds quality and in chemical attributes of the soil of a production field in the Brazilian Cerrado

Journal of Seed Science ◽

10.1590/2317-1545v40n1182375 ◽

2018 ◽

Vol 40 (1) ◽

pp. 25-35 ◽

Cited By ~ 1

Author(s):

Danielle Helena Müller ◽

Elisangela Clarete Camili ◽

Walcylene Lacerda Matos Pereira Scaramuzza ◽

Maria Cristina de Figueiredo e Albuquerque

Keyword(s):

Spatial Variability ◽

Seed Production ◽

Seed Mass ◽

Soybean Seed ◽

Accelerated Aging ◽

Exchange Capacity ◽

Soybean Seeds ◽

Distribution Maps ◽

Chemical Attributes ◽

Definition Of

Abstract: The objective of this study was to evaluate the spatial variability in soybean seeds quality and in soil chemical attributes of a production field. Data were collected at 138 georeferenced points of a soybean production property located in Santo Antônio de Leverger - MT. Soil related variables, such as phosphorus, potassium, calcium, magnesium, and organic matter (OM) contents, pH, cation exchange capacity (CEC) and base saturation (V%) were evaluated. On the other hand, yield, one thousand seed mass, size, germination, emergence in seedbed, electrical conductivity, accelerated aging and tetrazolium reaction were evaluated as seed variables. The data were submitted to descriptive and geostatistical analysis, and the fit semivariogram parameters were used to elaborate spatial distribution maps of the analyzed variables. After the analysis, it was possible to conclude that there was spatial variability in the evaluated attributes for both seeds and soil related variables, indicating that the soybean seed production area can be divided into management zones, which allows the definition of areas to be harvested or discarded within a field of seed production.

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