Concentrated whey from block freeze concentration or milk-based ice creams on Bifidobacterium BB-12 survival under in vitro simulated gastrointestinal conditions

Limosilactobacillus reuteri INIA P572 is a strain able to produce the antimicrobial compound reuterin in dairy products, exhibiting a protective effect against some food-borne pathogens. In this study, we investigated some probiotic properties of this strain such as resistance to gastrointestinal passage or to colonic conditions, reuterin production in a colonic environment, and immunomodulatory activity, using different in vitro and in vivo models. The results showed a high resistance of this strain to gastrointestinal conditions, as well as capacity to grow and produce reuterin in a human colonic model. Although the in vitro assays using the RAW 264.7 macrophage cell line did not demonstrate direct immunomodulatory properties, the in vivo assays using a Dextran Sulphate Sodium (DSS)-induced colitic mice model showed clear immunomodulatory and protective effects of this strain.

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Impact of 2′-Fucosyllactose on Gut Microbiota Composition in Adults with Chronic Gastrointestinal Conditions: Batch Culture Fermentation Model and Pilot Clinical Trial Findings

Nutrients ◽

10.3390/nu13030938 ◽

2021 ◽

Vol 13 (3) ◽

pp. 938

Author(s):

Jennifer Joan Ryan ◽

Andrea Monteagudo-Mera ◽

Nikhat Contractor ◽

Glenn R. Gibson

Keyword(s):

Ulcerative Colitis ◽

Batch Culture ◽

Pilot Trial ◽

Gastrointestinal Symptoms ◽

Short Chain Fatty Acids ◽

Open Label ◽

Gastrointestinal Conditions ◽

Fermentation Model ◽

Quality Of Life Index

Intestinal dysbiosis has been described in patients with certain gastrointestinal conditions including irritable bowel syndrome (IBS) and ulcerative colitis. 2′-fucosyllactose (2′-FL), a prebiotic human milk oligosaccharide, is considered bifidogenic and butyrogenic. To assess prebiotic effects of 2′-FL, alone or in combination with probiotic strains (potential synbiotics), in vitro experiments were conducted on stool from healthy, IBS, and ulcerative colitis adult donors. In anaerobic batch culture fermenters, Bifidobacterium and Eubacterium rectale-Clostridium coccoides counts, and short-chain fatty acids (SCFAs) including butyrate increased during fermentation with 2′-FL and some of the 2′-FL/probiotic combinations. In a subsequent open-label pilot trial, the effect of a 2′-FL-containing nutritional formula was evaluated in twelve adults with IBS or ulcerative colitis. Gastrointestinal Quality of Life Index (GIQLI) total and gastrointestinal symptoms domain scores, stool counts of Bifidobacterium and Faecalibacterium prausnitzii, and stool SCFAs including butyrate, increased after six weeks of intervention. Consistent with documented effects of 2′-FL, the batch culture fermentation experiments demonstrated bifidogenic and butyrogenic effects of 2′-FL during fermentation with human stool samples. Consumption of the 2′-FL-containing nutritional formula by adults with IBS or ulcerative colitis was associated with improvements in intra- and extra-intestinal symptoms, and bifidogenic and butyrogenic effects.

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Stem cells and exosomes: promising candidates for necrotizing enterocolitis therapy

Stem Cell Research & Therapy ◽

10.1186/s13287-021-02389-4 ◽

2021 ◽

Vol 12 (1) ◽

Author(s):

Ruijie Zeng ◽

Jinghua Wang ◽

Zewei Zhuo ◽

Yujun Luo ◽

Weihong Sha ◽

...

Keyword(s):

Stem Cells ◽

Necrotizing Enterocolitis ◽

Therapeutic Effects ◽

Beneficial Effects ◽

Pediatric Diseases ◽

Gastrointestinal Conditions ◽

Different Types ◽

Novel Therapeutic Strategies

AbstractNecrotizing enterocolitis (NEC) is a devastating disease predominately affecting neonates. Despite therapeutic advances, NEC remains the leading cause of mortality due to gastrointestinal conditions in neonates. Stem cells have been exploited in various diseases, and the application of different types of stem cells in the NEC therapy is explored in the past decade. However, stem cell transplantation possesses several deficiencies, and exosomes are considered potent alternatives. Exosomes, especially those derived from stem cells and breast milk, demonstrate beneficial effects for NEC both in vivo and in vitro and emerge as promising options for clinical practice. In this review, the function and therapeutic effects of stem cells and exosomes for NEC are investigated and summarized, which provide insights for the development and application of novel therapeutic strategies in pediatric diseases. Further elucidation of mechanisms, improvement in preparation, bioengineering, and administration, as well as rigorous clinical trials are warranted.

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Effect of physical and physicochemical characteristics of chitosan on fat-binding capacities under in vitro gastrointestinal conditions

LWT ◽

10.1016/j.lwt.2016.03.013 ◽

2016 ◽

Vol 71 ◽

pp. 25-32 ◽

Cited By ~ 16

Author(s):

Nootchanartch Panith ◽

Jetsada Wichaphon ◽

Sittiwat Lertsiri ◽

Nuttawee Niamsiri

Keyword(s):

Physicochemical Characteristics ◽

Gastrointestinal Conditions

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Potential of Inulin-Fructooligosaccharides Extract Produced from Red Onion (Allium cepa var. viviparum (Metz) Mansf.) as an Alternative Prebiotic Product

Plants ◽

10.3390/plants10112401 ◽

2021 ◽

Vol 10 (11) ◽

pp. 2401

Author(s):

Jakkrit Aisara ◽

Pairote Wongputtisin ◽

Somkid Deejing ◽

Chutamas Maneewong ◽

Kridsada Unban ◽

...

Keyword(s):

Pathogenic Bacteria ◽

Probiotic Bacterium ◽

Degree Of Polymerization ◽

Inhibitory Effects ◽

Commercial Cultivation ◽

Gastrointestinal Conditions ◽

Free Glucose ◽

Probiotic Lactobacilli ◽

Bifidogenic Effect

Red onion is a popular ingredient in many Thai dishes and has recently been promoted for commercial cultivation. In this study, inulin-fructooligosaccharides (inulin-FOSs) were extracted from red onions in a simplified extraction method. The extract contained 24.00 ± 0.38 g/L free glucose, fructose and sucrose, while the level of FOSs was recorded at 74.0 ± 2.80 g/L with a degree of polymerization of 4.1. The extract was resistant to simulated gastrointestinal conditions, while selectively promoting probiotic lactobacilli. These outcomes resulted in inhibitory effects against various pathogenic bacteria. The in vitro batch culture fermentation of the extract by natural mixed culture indicated that an unknown sugar identified as neokestose was more rapidly fermented than 1-kestose and other longer-chain inulin-FOSs. Notably, neokestose selectively encouraged a bifidogenic effect, specifically in terms of the growth of Bifidobacteirum breve, which is an infant-type probiotic bacterium. This is the first report to state that neokestose could selectively enhance the bifidogenic effect. In summary, inulin-FOSs extract should be recognized as a multifunctional ingredient that can offer benefits in food and pharmaceutical applications.

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The antibacterial activity expressed in vitro and in vivo by Lactococcus lactis subsp. lactis CNRZ 1427 against Salmonella sp.

South Asian Journal of Experimental Biology ◽

10.38150/sajeb.4(5).p240-246 ◽

2015 ◽

Vol 4 (5) ◽

pp. 240-246

Author(s):

Menad Najett ◽

Chougrani Fadelaa ◽

Moghtet Snoussi ◽

Cheriguene Abderrahim

Keyword(s):

Antibacterial Activity ◽

Lactococcus Lactis ◽

Bile Salts ◽

Therapeutic Agent ◽

Lactococcus Lactis Subsp ◽

Beneficial Effects ◽

Gastrointestinal Conditions ◽

Therapeutic Possibilities

The present study focused on the beneficial effects of Lactococcus lactis subsp lactis CNRZ 1427 with possible use as a therapeutic agent against Sal-monella sp.; also we have proposed different therapeutic possibilities of our situation against a pathogen Salmonella sp. We have conducted two tests In Vitro and In Vivo; where it is noted that treatment in the presence of this lac-tic strain is effective since it causes a remarkable decrease of the pathogen agent. At the end, the effectiveness of this lactic strain was confirmed by testing for resistance to gastrointestinal conditions (pH, bile salts and diges-tive enzymes).

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Listeria monocytogenes survival in raw Atlantic salmon (Salmo salar) fillet under in vitro simulated gastrointestinal conditions by culture, qPCR and PMA-qPCR detection methods

LWT ◽

10.1016/j.lwt.2019.03.015 ◽

2019 ◽

Vol 107 ◽

pp. 132-137

Author(s):

Clarissa Barretta ◽

Silvani Verruck ◽

Bruna Marchesan Maran ◽

Leticia dos Santos Maurício ◽

Marília Miotto ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Atlantic Salmon ◽

Salmo Salar ◽

Detection Methods ◽

Atlantic Salmon Salmo Salar ◽

Gastrointestinal Conditions

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Streptococcus thermophiles DMST-H2 Promotes Recovery in Mice with Antibiotic-Associated Diarrhea

Microorganisms ◽

10.3390/microorganisms8111650 ◽

2020 ◽

Vol 8 (11) ◽

pp. 1650

Author(s):

Jin-Shuang Hu ◽

Yan-Yan Huang ◽

Jia-Hua Kuang ◽

Jia-Jia Yu ◽

Qin-Yu Zhou ◽

...

Keyword(s):

Inflammatory Cytokines ◽

Intestinal Flora ◽

Intestinal Barrier ◽

Genomic Analysis ◽

Acid Tolerance ◽

Interferon Γ ◽

Common Side Effect ◽

Gastrointestinal Conditions ◽

Antibiotic Associated Diarrhea

Antibiotic-associated diarrhea (AAD) is the most common side effect of antibiotics and is routinely treated with probiotics in clinical. Streptococcus thermophiles, extensively utilized for producing dairy foods, has recently been regarded as a new promising probiotic candidate. In this study, the efficacy of Streptococcus thermophiles DMST-H2 (DMST-H2) for AAD treatment in mice was investigated. DMST-H2 was isolated from Chinese traditional yogurt, proved to be non-toxic, and presented tolerance against simulated gastrointestinal conditions in vitro. Additionally, genomic analysis revealed that it possessed genes related to acid tolerance, bile salt tolerance, adhesion, oxidative stress and bacteriocin production. The animal experiment results showed that both DMST-H2 treatment and natural recovery could reduce fecal water content. Compared with spontaneous recovery, DMST-H2 accelerated the recovery of the enlarged caecum and intestinal barrier injury from AAD, and further decreased endotoxin (ET), D-lactate (D-LA) and diamine oxidase (DAO) content in serum. Moreover, pro-inflammatory cytokines (TNF-α) were reduced, while interferon-γ (IFN-γ) and anti-inflammatory cytokines (IL-10) increased after treating with DMST-H2. Furthermore, DMST-H2 better restored the structure of intestinal flora. At the phylum level, Firmicutes increased and Proteobacteria decreased. These findings indicate that DMST-H2 could promote recovery in mice with antibiotic-associated diarrhea.

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