scholarly journals Intralesional polysulphated glycosaminoglycan as treatment of equine collagenase induced tendinitis: clinical, ultrasonographic and histopathologic evaluation

2004 ◽  
Vol 56 (6) ◽  
pp. 701-708 ◽  
Author(s):  
S. Marxen ◽  
J.C. Lacerda Neto ◽  
J.C. Canola ◽  
J.R.E. Moraes ◽  
G. Ribeiro
Keyword(s):  
Intralesional Injection ◽  
Control Groups ◽  
Beneficial Effects ◽  
Thoracic Limb ◽  
Superficial Digital Flexor Tendon ◽  
Histopathologic Evaluation ◽  
Intralesional Injections ◽  
Males And Females ◽  
Histopathologic Analysis ◽  
And Control

The effect of intratendineous injections of polysulphated glycosaminoglycan (PSGAG) as treatment of collagenase-induced tendonitis was studied. Two groups (GI and GII) of five Arabian horses each, males and females, two to six year-old, were submitted to experimental tendinitis of the superficial digital flexor tendon of the left thoracic limb by intratendineous injection of 1.0ml of collagenase (2.5mg/ml). Seven days after the induced-lesions were created, the horses of GI received five intralesional injections of 1.0ml (125mg) of PSGAG, every four days. Horses of GII received injections of saline in the same dose and rate. Clinical and ultrasonographic evaluations were performed periodically, during 150 days. All animals showed lameness, increased local pain, heat and swelling 24 hours after the injury was created. All signs, except to swelling, which remained visible by the end of the study, showed regression in all animals. Lesions of variable size, shape and position were evidenced by the ultrasonographic evaluation, reaching maximum severity between the seventh and the 23rd days. By the end of the study, the echogenicity grade ranged from 1 to 2, and the grade of fiber alignment from 0 to 2. The histopathologic analysis demonstrated repair areas with intense fibroplasia and neovascularization, collagen fibers poorly organized, and thickened hypercellular endotenon. The data of this study did not show significant differences between the treated and control groups, therefore leading to the conclusion that the intralesional injection of PSGAG did not have beneficial effects in the treatment of collagenase-induced tendinitis.

Effects of 4 Weeks of Elastic-Resistance Training on Ankle-Evertor Strength and Latency

2011 ◽  
Vol 20 (2) ◽  
pp. 157-173 ◽  
Author(s):  
KyungMo Han ◽  
Mark D. Ricard
Keyword(s):  
Exercise Training ◽  
Resistance Exercise ◽  
Outcome Measures ◽  
Ankle Sprains ◽  
Control Groups ◽  
Resistance Exercise Training ◽  
Peroneus Longus ◽  
Elastic Resistance ◽  
Males And Females ◽  
And Control

Context:Several researchers have suggested that improving evertor strength and peroneus longus reaction time may help alleviate the symptoms of chronic ankle instability and reduce the rate of recurrent ankle sprains.Objectives:To determine the effectiveness of a 4-wk elastic-resistance exercise-training program on ankle-evertor strength and peroneus longus latency in subjects with and without a history of ankle sprains (HAS).Design:Randomized controlled clinical trial.Participants:40 subjects (20 male, 20 female; 20 HAS, 20 healthy). Ten subjects (5 male and 5 female) from each of the HAS and healthy groups were randomly assigned to exercise or control groups.Interventions:4-directional elastic-resistance exercise training 2 times/wk for 4 wk.Main Outcome Measures:Ankle-evertor strength and peroneal muscle latency after sudden inversion were measured before training, after 4 wk of training, and 4 wk posttraining.Results:Four weeks of elastic-resistance exercise training did not elicit significant changes in 1-repetition-maximum ankle-evertor strength between the exercise and control groups (P = .262), HAS and healthy groups (P = .329), or males and females (P = .927). Elastic-resistance exercise training did not elicit significant changes in peroneus longus muscle latency between the exercise and control groups (P = .102), HAS and healthy groups (P = .996), or males and females (P = .947).Conclusions:The 4-wk elastic-resistance exercise training had no effect on ankle-evertor strength and reflex latency of the peroneus longus after unexpected ankle inversion.

scholarly journals A comparative biomechanical study of repaired tendons wrapped with two biological matrices in Bucks

2016 ◽  
Vol 40 (1) ◽  
pp. 73-78
Author(s):  
N. H. AL-Falahi
Keyword(s):  
Extracellular Matrix ◽  
Tensile Strength ◽  
Matrix Group ◽  
Biological Matrices ◽  
Control Groups ◽  
Platelet Rich Fibrin ◽  
Fibrin Matrix ◽  
Superficial Digital Flexor Tendon ◽  
And Control ◽  
Autologous Platelet

     This study is planned to evaluate the efficacy of two biological matrices represented by autologous platelet rich fibrin matrix, as well as a cross linked decellularized caprine pericardial extracellular matrix on enhancing healing of the experimentally severed superficial digital flexor tendon in a goat model. It was carried out on 48 adult apparently healthy bucks, which were divided randomly into three equal groups. Under the effect of sedative and local ring block anesthesia, superficial digital flexor tendon was severed at the mid metacarpal region of the right forelimb. In the first control group, tenorrhaphy was performed and left without additives. While in the second group the tenorrhaphy site was wrapped with a previously prepared autologous platelet rich fibrin strips, as well as in the third group the tenorrhaphy site was wrapped with a cross linked decellularized pericardial extracellular matrix strip which was prepared from the whole fresh caprine pericardium obtained from the slaughter house. Both matrices were fixed in their position at the tenorrhaphy site by few interrupted stitches. The biomechanical evaluation of the operated tendon indicated an increase in tensile strength with time in all groups, but the comparisons among groups showed a significant (P≤0.05) increase at day 15 in both treated as compared to control animals. On day 45 the pericardial extracellular matrix group showed a significant increase in tensile strength as compared to platelet rich fibrin matrix and control groups, but at day 75 there were no differences among groups, at day 180 the pericardial extracellular matrix group showed a significant increase in the tensile strength as compared to platelet rich fibrin matrix and control groups. In conclusion, both biological matrices led to improvement in the biomechanical properties of the operated tendons with time.

scholarly journals Biomarkers in Community-Acquired Pneumonia Assessment

2017 ◽  
Vol 71 (1-2) ◽  
pp. 8-13
Author(s):  
Natalja Voskresenska ◽  
Sergejs Babikovs ◽  
Jūlija Voicehovska ◽  
Andrejs Šķesters ◽  
Alise Silova ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzymes ◽  
Community Acquired Pneumonia ◽  
Treatment Efficiency ◽  
Control Groups ◽  
Plasma Selenium ◽  
Healthy Control ◽  
Males And Females ◽  
And Control ◽  
Reduced Activity

AbstractThe paper presents information on pneumonia (P) patients with features of oxidative stress (OS). Identifying features of OS in patients with P is of interest not only for diagnosis, but also for monitoring of treatment efficiency. We recruited 73 patients with community-acquired P (CAP), previously healthy adults, both males and females with mean age of 68.0 ± 15.2, hospitalised, and 61 healthy control patients matched for age. For quantitative evaluation of lipid peroxidation in CAP patients, the levels of aldehydic lipid peroxidation products like malondialdehyde (MDA) and 4- hydroxynon-2-enal (HNE) were quantified. Furthermore, concentrations of reduced glutathione (GSH) and several antioxidant enzymes and selenium in plasma were determined. In CAP patients, decreased levels of GSH and plasma selenium were observed. Plasma levels of MDA, and HNE did significantly differ between patient and control groups. We also noted reduced activity of antioxidant enzymes, namely, glutation peroxidase and superoxide dismutase. Low antioxidant enzymes activity was associated with a more severe CAP pattern. Both GSH and antioxidant enzymes may serve as markers for inflammation-related OS in CAP patients, and measurement of these biomarkers may be a valid indentifier for its management.

scholarly journals Movements and Homing of Cutthroat Trout (Salmo clarki) from Open-Water Areas of Yellowstone Lake

1969 ◽  
Vol 26 (5) ◽  
pp. 1243-1261 ◽  
Author(s):  
Lawrence A. Jahn
Keyword(s):  
Cutthroat Trout ◽  
Open Water ◽  
East Side ◽  
Control Groups ◽  
The West ◽  
Yellowstone Lake ◽  
Males And Females ◽  
And Control ◽  
Spawning Tributaries ◽  
Salmo Clarki

Cutthroat trout (Salmo clarki) showed in-season homing after displacement from their spawning tributaries to Yellowstone Lake during June–August 1966 and 1967. Of 300 nonanesthetized trout tagged and displaced from Clear and Cub creeks to three release points (0.5–22.0 km) in the lake and to the mouth of Clear Creek, 38.3% homed, 10.0% strayed, 2.7% were caught by anglers, and the remainder were unaccounted for. Anosmic (olfactory chambers plugged) and blind-anosmic fish homed in significantly lower percentages than nonanesthetized and control groups. Fish released just outside the mouth of the homestream had the shortest average homing time, but the average homing time for fish displaced 22.0 km from the homestream was shorter than for those displaced 5.0 km away. Homing percentages for trout tagged after being tracked individually in the open lake were similar to those for trout in the group tagging experiments, but the former had longer average homing times than the latter.The directions of orientation of fish tracked in open water were generally toward the homestream, the directions usually coinciding with those of the sun azimuth. However, fish taken from the east side of the lake went west-northwest when tracked late in the afternoon and fish taken from the west side of the lake went east-southeast when tracked in the morning, these directions being away from those of the homestreams. Orientation was affected by current. Mean directions for males and females were generally not significantly different. Average swimming speeds and vector lengths for males and females were about the same. Immature cutthroat trout were trained to use a light source as a reference point for orientation.

scholarly journals Mesenchymal stem cell-derived exosomes protect trabecular meshwork from oxidative stress

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ying-chao Li ◽  
Juan Zheng ◽  
Xi-zi Wang ◽  
Xin Wang ◽  
Wen-jing Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Survival Rate ◽  
Candidate Genes ◽  
Inflammatory Cytokines ◽  
Trabecular Meshwork ◽  
Control Groups ◽  
Beneficial Effects ◽  
Protein Levels ◽  
Trabecular Meshwork Cells ◽  
And Control

AbstractThis study aims to investigate the beneficial effects of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on trabecular meshwork cells under oxidative stress and predict candidate genes associated with this process. Trabecular meshwork cells were pretreated with BMSC-derived exosomes for 24 h, and exposed to 0.1 mM H2O2 for 6 h. Survival rate of trabecular meshwork cells was measured with CCK-8 assay. Production of intracellular reactive oxygen species (iROS) was measured using a flow cytometer. RT-PCR and ELISA were used to detect mRNA and protein levels of inflammatory cytokines and matrix metalloproteinases (MMPs). Sequencing of RNA and miRNA for trabecular meshwork cells from Exo and control groups was performed on BGISEQ500 platform. Phenotypically, pretreatment of BMSC-derived exosomes improves survival rate of trabecular meshwork cells exposed to H2O2, reduces production of iROS, and inhibits expression of inflammatory cytokines, whereas increases expression of MMPs. There were 23 miRNAs, 307 lncRNAs, and 367 mRNAs differentially expressed between Exo and control groups. Exosomes derived from BMSCs may protect trabecular meshwork cells from oxidative stress. Candidate genes responsible for beneficial effects, such as DIO2 and HMOX1, were predicted.

scholarly journals Methoprene and Temperature Stimulation of Emergence and Longevity in Adults of Osmia Rufa L. (Megachilidae; Apoidea) During Wintering Period

2017 ◽  
Vol 61 (1) ◽  
pp. 139-147
Author(s):  
Karol Giejdasz ◽  
Oskar Wasielewski
Keyword(s):  
Juvenile Hormone ◽  
Sucrose Solution ◽  
Adult Males ◽  
Control Groups ◽  
Juvenile Hormone Analogues ◽  
Males And Females ◽  
Rate Of Emergence ◽  
And Control ◽  
Temperature Stimulation ◽  
Stimulation Of

Abstract In this study methoprene, a juvenile hormone analogues, was tested as a factor that stimulates the end of diapause, bee activation and emergence. In addition, the survival of bees was checked when combined with an application of methoprene. The experimental activation of wintering bees was carried out once a month between December and March, Three groups of bee cocoons were selected for each activation term: treated with either methoprene or acetone as vehicle control and untreated as controls. After the applications were finished the cocoons were incubated at temperature 26°C. The emerged adult males and females were then kept in the laboratory and fed sucrose solution to evaluate the longevity of bees. The application of juvenile hormone analogue reduced the emergence time of adult bees in December, February and March. The rate of emergence presented in the form of cumulative percentage of emerged adult bees indicated that the bees treated with methoprene started to emerge 1-2 days earlier than bees from acetone and control groups and finished emergence 2-5 days earlier too. Methopren application did not reduce the longevity of the bees after emergence. Moreover, the median longevity of these females was higher than untreated in December and February.

The inheritance of thiabendazole resistance in Haemonchus contortus

Parasitology ◽  
1979 ◽  
Vol 78 (2) ◽  
pp. 107-119 ◽  
Author(s):  
L. F. Le Jambre ◽  
W. M. Royal ◽  
P. J. Martin
Keyword(s):  
Dose Response ◽  
Haemonchus Contortus ◽  
Inheritance Of Resistance ◽  
Dominant Trait ◽  
Control Groups ◽  
Resistant Parent ◽  
Back Cross ◽  
Males And Females ◽  
And Control

SUMMARYHaemonchus contortus worm populations isolated from naturally infected sheep at the Pastoral Research Laboratory, Armidale, N.S.W., were found to contain approximately 20% of worms resistant to a 50 mg/kg dose of thiabendazole. Following 3 generations of selection with 50 mg/kg thiabendazole the number of worms removed by the anthelmintic was too small to detect differences between treated and control groups. After more than 15 generations of selection, matings between males from the selected strain and non-resistant females produced resistant males and females in equal numbers. Thus, thiabendazole resistance does not appear to be sex-linked. A dose–response assay on the F2 adults indicated that worms from female resistant × male non-resistant crosses were more resistant than F2 adults of the reciprocal cross. An in vitro technique that identified thiabendazole-resistant eggs by their ability to hatch in a solution containing thiabendazole and 0·1% NaCl solution was also used to study the inheritance of resistance. F1 eggs had similar LC50's to the resistant parents. F2 and back-cross eggs from an original mating of thiabendazole-resistant females × non-resistant males had a higher LC50 than F2 and back-cross eggs from the reciprocal mating, indicating a degree of matroclinous inheritance of resistance. However, the resistant parents had tolerances to thiabendazole exceeding those of F2. F3 eggs had a resistance distribution that ranged from that of the resistant to the non-resistant parent. No significant deviation from linearity was observed in any of the dose–response lines. These results indicate that thiabendazole resistance in H. contortus worms is inherited as an autosomal and semi-dominant trait.

COMPARATIVE TREATMENT OF ACNE VULGARIS USING PALOMAR LUX APPLIQUÉ TECHNIQUE AND DIRECT INTRALESIONAL INJECTION

2009 ◽  
Vol 02 (03) ◽  
pp. 279-287
Author(s):  
ELINA A. GENINA ◽  
ALEXEY N. BASHKATOV ◽  
VALERY V. TUCHIN ◽  
GEORGY V. SIMONENKO ◽  
VERA N. SHERSTNEVA ◽  
...  
Keyword(s):  
Thermal Damage ◽  
Acne Vulgaris ◽  
Skin Permeability ◽  
Intralesional Injection ◽  
Control Groups ◽  
Promising Technique ◽  
Comparative Treatment ◽  
And Control ◽  
Narrow Class

Intralesional injection of triamcinolone (TMC) preparations is an effective therapy for cystic acne lesions. However, invasive delivery techniques limit the use of this modality to a relatively narrow class of cases. Skin permeability can be enhanced through creating a lattice of micro-zones (islets) of light-induced limited thermal damage in the upper layers of epidermis. In this paper, we directly compared safety and efficacy of delivering TMC acetonide with this novel technique versus conventional intralesional injection for treatment of inflammatory acne lesions. A combination of an intense pulsed light system and a specially designed appliqué with a pattern of absorbing centers has been used to create the lattice of islets of damage (LID). Quantitative analysis has included estimation of the following parameters: redness, diameter, and height of acne lesions. Clinical photography has been used to document dynamics of lesion development at successive visits (two hours, 24 hours and one week post-treatment). Seven subjects have participated in the study. No difference in lesion dynamics between the treatment and control groups was observed at two-hours follow-up. At 24-hours/one-week follow-ups, TMC-injected and TMC-LID-delivered groups have demonstrated 82%/93% and 80%/89% improvement in height of lesions in comparison to control (60%/68%). Delivery of TMC with the newly proposed LID technique is at least as effective as intralesional injection for treating inflammatory acne lesions. Enhancement of skin permeability using LID approach is a promising technique for accelerating delivery of various compounds to their target areas in the skin.

scholarly journals Mesenchymal Stem Cell-Derived Exosomes Protect Trabecular Meshwork from Oxidative Stress

2021 ◽  
Author(s):  
Ying-chao Li ◽  
Juan Zheng ◽  
Xi-zi Wang ◽  
Xin Wang ◽  
Wen-jing Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Survival Rate ◽  
Candidate Genes ◽  
Inflammatory Cytokines ◽  
Trabecular Meshwork ◽  
Control Groups ◽  
Beneficial Effects ◽  
Protein Levels ◽  
Trabecular Meshwork Cells ◽  
And Control

Abstract This study aims to investigate potential effects of exosomes derived from bone marrow mesenchymal stem cells (BMSCs) on trabecular meshwork cells under oxidative stress and predict candidate genes associated with this process. Trabecular meshwork cells were pretreated with BMSC-derived exosomes, and exposed to 0.1mM H2O2. Survival rate of trabecular meshwork cells was measured with CCK-8 assay. Production of iROS was measured using a flow cytometer. RT-PCR and ELISA were used to detect mRNA and protein levels of inflammatory cytokines and matrix metalloproteinases (MMPs). RNA sequencing for trabecular meshwork cells from Exo- and control groups was performed on BGISEQ500 + bgiseq-500 platform. Phenotypically, pretreatment of BMSC-derived exosomes improves survival rate of trabecular meshwork cells exposed to H2O2, reduces production of iROS, and inhibits expression of inflammatory cytokines, whereas increases expression of MMPs. There are 23 DEmiRNAs, 307 DElncRNAs, and 367 DEmRNAs differentially expressed between Exo- and control groups. Exosomes derived from BMSCs may protect trabecular meshwork cells from oxidative stress. Candidate genes responsible for beneficial effects ,such as DIO2 and HMOX1, such as DIO2 and HMOX1, are predicted.

scholarly journals Cellular, Humoral, and Histopathologic Analysis in Rats Implanted with Orthodontic Nickel Brackets

2008 ◽  
Vol 78 (1) ◽  
pp. 114-119 ◽  
Author(s):  
Cássio Vicente Pereira ◽  
Estela Kaminagakura ◽  
Paulo Rogério Ferreti Bonan ◽  
Renata Avelar Bastos ◽  
Luciano José Pereira
Keyword(s):  
Subcutaneous Tissue ◽  
Biological Effects ◽  
Orthodontic Appliances ◽  
Control Groups ◽  
Significant Difference ◽  
Leukocyte Number ◽  
Histopathologic Analysis ◽  
Group B ◽  
And Control ◽  
Histopathologic Findings

Abstract Objective: To determine the immune response related to nickel and nickel-free orthodontic appliances using cellular (total and differential leukocytes) and humoral (IgA) quantification. Materials and Methods: Thirty-five Wister rats were randomly distributed into three groups (A, B, and C). In the A group, the animals were subdivided into three subgroups (A1: control, without brackets; A2: nickel-free brackets; and A3: nickel brackets) with seven animals in each group; the animals were sacrificed after 14 days. Groups B and C received only nickel brackets and were kept for 14 days. After this period, the implants were removed and the rats were sacrificed. In group B, the animals were sacrificed 2 days after the appliance removal (16 days in total); and in group C, the animals were sacrificed 7 days after the brackets removal (21 days in total). Total and differential leukocyte number, IgA quantification and histopathologic analysis were done. Histopathologic analysis of subcutaneous tissue related to the orthodontic brackets was done as well. Results: There were significant differences between the number of leukocytes for the nickel-implanted animals and the nickel-free and control groups after 14 days of implantation (P < .05). However, there was no significant difference among groups A1, A2, and A3 for the differential number of leukocytes and for the IgA quantification, except for the number of monocytes, which was three times higher in the nickel group (A3). The histopathologic findings did not show differences between groups. Conclusions: Nickel did not promote significant adverse biological effects.

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