The Relationship Between Leukocytes Numbers and Consciousness Level of Craniotomy Patients at The Jemursari Islamic Hospital Surabaya in 2018-2019

This study investigates the correlation between leukocyte number and the patient's awareness level after craniotomy surgery, both high (leukocytosis) and low (leukopenia). Several studies reveal that an increase in leukocytes affects mortality rates due to the high level of leukocytes affecting our body's functionalities, including awareness. Previous studies revealed that a leukocyte count exceeding 17.5x106/L was associated with a lower GCS score, a longer hospital stay, and worsening CT scan results, regardless of the type of focal lesion that occurred. This study uses observational methods in the form of retrospective case studies. The data was collected from the medical records of the Operation room at Jemursari Islamic Hospital in 2018-2019 with a sample size of 89. The research was conducted at Jemursari Islamic Hospital Surabaya from May 2018 to August 2019. Data were analyzed using the Spearman correlation test with p <0.05. Results showed no significant relationship between the leukocyte count and the consciousness level of post-craniotomy patients. Future research could estimate the specific effects on the morphology of specific leukocyte cells that are elevated in post-craniotomy patients. This research is expected to provide insight into the effect of leukocytes on consciousness to reduce patient mortality after craniotomy.

Aesculetin Inhibits TLR4 and EGFR-Mediated Airway Mucus Hypersecretion and Thickening Induced by Urban Particulate Matter

Objectives uPM10 is microscopic particles of solid or liquid matter suspended in the air. Particulates are the most harmful form of air pollution due to their ability to penetrate deep into the lungs, blood streams and brain, causing diverse health problems. Mucus hypersecretion is a pathological symptom evoked in the bronchi and bronchioles. Excess mucus production can narrow the airways, limit airflow, and accelerate a decline in lung function. Aesculetin, a major component of Sancho tree and Chicory, is known to have antioxidant and anti-inflammatory effects in the vascular and immune system. However, its effect on mucus hypersecretion has been poorly understood. Methods Mice were orally administrated with 10 mg/kg aesculetin and exposed to 6 μg/ml uPM10 for 8 weeks. Airway thickening and collagen accumulation were stained with H&E staining and Masson trichrome staining. The ROS production and mucus hypersecretion were examined with DHE staining, Alcian blue, periodic acid schiff staining and Western blotting. Bronchial epithelial BEAS-2B cells were treated 1–20 μM aesculetin, 20 μg/ml OxPAPC, and 1μM Erlotinib with 2 μg/ml uPM10 for 8 h or 48 h. The protein induction of TLR4, EGFR, and MUC5AC were measured. Results Exposure of mice to uPM10 enhanced total leukocyte number in the BALF with increase in neutrophils and lymphocytes, which was reversed by oral administration of 10 mg/kg aesculetin. In addition, aesculetin attenuated airway thickening along with collagen accumulation, mucus hypersecretion and ROS production elevated by uPM10 inhalation. Nontoxic aesculetin at the concentrations of 1–20 μM inhibited the induction of collagen proteins and MUC5AC in BEAS-2B cells promoted by 2 μg/ml uPM10. On the other hand, uPM10 accelerated the induction of TLR4 and EGFR in BEAS-2B cells, which dampened by 20 μM aesculetin. Both receptors inhibitor reduced the MUC5AC induction in BEAS-2B cells stimulated by uPM10. Conclusions These results demonstrate that aesculetin ameliorated airway thickening and mucus hypersecretion caused by uPM10 inhalation. Therefore, aesculetin maybe a promising agent treating progressive respiratory disorders elicited by urban microparticulates. Funding Sources This work was supported by the BK21 FOUR(Fostering Outstanding Universities for Research, 4220200913807) funded by the National Research Foundation of Korea (NRF).

Prevalence of diarrhea of goats and their concurrent blood profile in north west region of Bangladesh

In Bangladesh, Diarrhea occurs in goats of any age and is responsible for poor growth in kids and a significant loss of production both through morbidity and mortality. The study was undertaken to determine the clinical prevalence of diarrheal diseases of goats with concurrent blood profile. A total of 1,007 goats were examined which were brought for treatment in the Upazilla Veterinary Hospital, Biral, Dinajpur. Among them 149 goats were found clinically affected by diarrhea and the overall prevalence of diarrheal disease in goats was 14.80%. A total of 402 male and 605 female goats were examined and their diarrheal prevalence were 14.17%, 15.15%, respectively. The Prevalence in Jamnapari goat (18.80%) was signicantly (P<0.01) higher than in Black Bengal goats (14.26%). The prevalence (59.52%) in suckler (below 3 months) was much significantly (P<0.001) higher than in young (12.47%) and adults (13.42%). Higher prevalence was found due to parasitic infection (38.25%) with a declining order in PPR (30.20%), overfeeding (24.16%) and poisoning (7.30%). The leukocyte number significantly (P<0.001) higher in goats affected by diarrhea whereas TEC, PCV, Hb and Erythrocyte Indices were in normal ranges. From this study, it can be concluded that suckler, female goats and Jamnapari goats are more susceptible to diarrhea than adult, male goats and Black Bengal goats. Asian J. Med. Biol. Res. March 2021, 7(1): 6-11

Blood Leukocytes in Young and Aged Rats After Whole Body Cryostimulation (–120°C)

The whole body cryostimulation (WBC) (–120°C) induces significant changes in blood leukocyte parameters in young and aged rats (total leukocyte number (leukocytosis or leukopenia); quantitative and qualitative correlation of their types, and shift direction in leukocyte formula); appearance of immature, plasm and polychromatophilic cells, cytoplasm plasmatization in some leukocytes, and changes in the integral leukocyte indices. The direction and severity of these changes depend on animals age (6–7 or 18–20 months old), number of the WBC sessions (1, 2 or 3), terms of observation (day and week) and mainly indicates physiological alterations in a body, that may be associated with the strain of involved functional systems and stress. In aged rats only, 24 hrs after the 2nd WBC session the lymphocyte percentage corresponded to a lower control value, thus indicating the training response formation at this stage. The integral leukocyte indices of aged rats underwent considerably smaller changes if compared with the young ones, especially in 24 hrs and a week after the WBC; the Garkavi adaptation index increased only in aged rats and just 24 hrs after the 3rd WBC session.

In Vitro and In Vivo Effects of Holotoxin A1 From the Sea Cucumber Apostichopus japonicus During Ionizing Radiation

Radiation therapy is one of the most important approaches to cancer therapy, but radiotoxicity to normal tissue is a serious limitation of this treatment. Compounds which are able to either sensitize cancer cells or protect normal cells to radiation are of great interest. The cytotoxicity of holotoxin A1 and the effects of radiation against DLD-1 and HT-29 cells were measured by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay. The effect of the combination of holotoxin A1 with X-ray on colony formation of cancer cells was determined by the soft agar assay. The effect of holotoxin A1 on the recovery of peripheral blood leukocyte number, mass, and cellularity of the lymphoid organs of irradiated mice, as well as on growth of murine Ehrlich solid carcinoma was studied. Holotoxin A1 enhanced the sensitivity of colorectal carcinoma cells to radiation in vitro. Injection of holotoxin A1 to mice led to an increase in the spleen endogenous colony number and peripheral blood leukocyte number, as well as the weight and cellularity of the lymphoid organs of the irradiated mice. Holotoxin A1 in combination with X-ray radiation effectively inhibited the growth of Ehrlich solid carcinoma in vivo. Holotoxin A1 is suggested to be a promising agent for improving the efficiency of radiotherapy.

C-reactive protein concentration in bipolar disorder: association with genetic variants

Background Several recent studies have investigated the role of C-reactive protein (CRP) in bipolar disorder (BD), but few studies have directly investigated the interaction between CRP genetic variants and peripheral CRP concentration across different phases of BD. In this study, we aimed to replicate previous findings that demonstrated altered CRP levels in BD, and to investigate whether there is an association of peripheral protein expression with genetic variants in the CRP gene. Methods 221 patients were included in the study, of which 183 (all episodes, 46 not medicated, 174 medicated) were genotyped for CRP single-nucleotide polymorphisms (SNPs) shown to influence peripheral CRP protein expression (rs1800947, rs2808630, rs1417938, rs1205). Results There were no differences in CRP levels associated with the genotypes, only regarding the rs1205 SNP there were significantly different CRP protein expression between the genotypes when taking body mass index, age, BD polarity, subtype and leukocyte number into account. However, we could show significantly elevated CRP protein expression in manic patients compared to euthymic and depressed patients, independent from genotype. Medication was found to have no effect on CRP protein expression. Conclusions These results indicate that low grade inflammation might play a role in mania and might be rather a state than a trait marker of bipolar disorder.

Age-associated and Breed-associated Variations in Haematological and Biochemical Variables in Mangalitsa, Mangalitsa×Durock and Large White Pig

Background: Research of hematologic and biochemical parameters in pigs is of great importance considering the fact that pigs are used as a model in research of different health disorders in humans. There are many different breeds of pigs that have different health, productive and biologic characteristics that need to be studied. Hematologic and biochemical values can vary dependent on presence of inflammation and infection.The aim of this study was to determine the influence of breed, age and their interactions on hematologic and biochemical parameters on blood of mangalitsa, mangalitsa x durock and Large White pig. Materials, Methods & Results: Experiment included 10 litters of mangalitsa, white variety, mated with mangalitsa boar, 10 mangalitsa litters, and white variety inseminated with durock boar and litters of great Large White inseminated with great Yorkshire boar. Six groups, each include 10 animals were formed and their blood was sampled (3 breeds and 2 age categories). Age groups were formed according to moment of blood sampling. First sample was taken in moment of 30 ± 5 kg of body weight. Second sample was taken when body weight was 100 kg hematologic analyze. Samples were taken with BD Vacutainer®. Complete classic blood analyzes and leucocytes formulas were done by hematology analyzer ADVIA 120 Hematology Siemens, Germany. Biochemical analyze was done by biochemical analyzer A15 BioSystem with their standard colorimetric reagenses. Concentration of total protein, albumin, urea, creatinine, cholesterol, total bilirubin, AST and ALT were determined. Globulin concentration was calculated. Results have showed that hematologic and biochemical parameters are influenced by breed, age and their interaction as: total leukocyte number (age, breed x age), neutrophils number (age, breed), number of monocytes and platelets (age, breed x age), eosinophils number and percentage (age), percentage of neutrophils, percentage of lymphocytes and cholesterol (breed). All of three factors (breed, age breed x age) have affected number of lymphocytes basophils number, % of monocytes, % of basophils, erythrocyte number, hemoglobin concentration, hematocrit, MCV, MCH, MCHC, RDW, protein, albumin, creatinine and ALT. Globulin value varied in function of interaction breed x age. RBC, Hgb, Hct, MCV, MCH, MCHC, total protein albumin, creatinine, and ALT variance can be explained by influence of breed, age and their interaction (η2 = 35-75%).Discussion: Based on results of this study breed and age have significant influence on numerous blood parameters. Besides the differences in mean values of parameters it is proved presence of statistically significant difference in frequencies distributions in function of age and breed. Different age and breed categories have different frequencies distributions of many determined parameters. There is positive correlation between RBC, Hgb, Hct and albumin in all three breed (R2 = 67-84%). Negative correlation was found between WBC and RBC, Hgb, Hct that was statistically significant in mangalitsa breed (R2 = 58-69%) but not in other two breeds. In mangalitsa significant positive correlation was found between globulin and leukocyte number. Given values have showed that during interpretation of lab results breed, age and interactions of hematologic and biochemical parameters need to be considered. Mean values and frequencies distribution differences lead to redefinition of referent range in function of breed and age that requires further research.