scholarly journals The influence of prolactin on bone mineral density (BMD) and some biochemical markers of ovariectomized rats

2010 ◽  
Vol 55 (No. 2) ◽  
pp. 83-88
Author(s):  
B. Dolińska ◽  
A. Suszka-Świtek ◽  
S. Dragan ◽  
F. Ryszka ◽  
R. Kołacz ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Bone Mineral ◽  
Biochemical Markers ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Osseous Tissue ◽  
Mineral Density ◽  
Healthy Group

A model of postmenopausal osteoporosis was used in the study. 107 days after ovariectomy the animals were subcutaneously applied 1.0 IU PRL/kg BM or 1.0 IU calcitonin/kg BM for 7 days in single doses. The application of the preparations started again 80 days after the first application. After 194 days the animals were narcotized, blood was collected, and lumbar (L2–L4) and femoral bones were prepared. The material obtained was subjected to densitometric analysis of bone mineral density (BMD). In ovariectomized rats the loss of osseous tissue was observed only in the lumbar bone. The application of prolactin to ovariectomized rats caused an increase in the mineral density of the lumbar bone up to a BMD value equal to that of the control group which had not been subjected to ovariectomy (control healthy group – SHAM). Similar results were obtained in the case of calcitonin. An increase in osteocalcin concentration and activity of isoenzyme alkaline phosphatase (BAL), with a decrease in the activity of isoenzyme acid phosphatase (TRAP) was observed in experimental groups compared to control ones.

scholarly journals Swim-trained rats have greater bone mass, density, strength, and dynamics

2001 ◽  
Vol 91 (4) ◽  
pp. 1663-1668 ◽  
Author(s):  
K. J. Hart ◽  
J. M. Shaw ◽  
E. Vajda ◽  
M. Hegsted ◽  
S. C. Miller
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Bone Structure ◽  
Mass Density ◽  
Weight Bearing ◽  
Femoral Shaft ◽  
Ovariectomized Rats ◽  
Bone Mass Density ◽  
Control Group ◽  
Mineral Density

Weight-bearing exercise is traditionally recommended for improving bone health in postmenopausal women. Effects of swim exercise were studied as an alternative to weight-bearing exercise in ovariectomized rats. Rats in a swim group (Sw, n = 8) swam for 12 wk, 5 days/wk for 60 min per session. A control group (Con, n = 9) engaged in no structured exercise. Femurs were analyzed for bone mineral density and for bone mineral content by dual energy X-ray absorptiometry, biomechanical properties by three-point bending (Instron), and bone structure and formation by histomorphometry. Food intake did not differ among groups. Final body weights were significantly lower in Sw compared with Con ( P< 0.05). Swimmers had significantly greater femoral shaft bone mineral density and content ( P < 0.05) compared with Con. Femurs of the Sw group had greater mechanical properties ( P< 0.05) compared with Con. Histomorphometric data were significantly better in the Sw group compared with Con after the 12-wk intervention ( P < 0.05). In conclusion, data from this study demonstrate some beneficial effects of swim exercise on bone structure, turnover, and strength.

scholarly journals Bone mineral density and bone metabolic markers in laboratory in men with marfanoid habitus

2017 ◽  
Vol 8 (6) ◽  
pp. 42-49
Author(s):  
Evgeny V. Timofeev ◽  
Tatiana I. Belousova ◽  
Elena V. Vutrih ◽  
Eduard V. Zemtsovsky ◽  
Andrey Yu. Olkhovik
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Tissue ◽  
Bone Mineral ◽  
Diagnostic Algorithm ◽  
Control Group ◽  
Mineral Density ◽  
Metabolic Markers ◽  
Power Shortages ◽  
Increased Activity

The aim of the research was the study of bone mineral density (BMD) and activity laboratory markers of bone metabolism in juniors with power shortages and marfanoid habitus (MH). Materials and methods: Twenty 119 males are underweight aged 18 to 25 years old (average age of 20.4 ± 1.5 years). All surveyed was conducted anthropometric, phenotypical, Echocardiography study to identify the mitral valve prolapse (MVP), laboratory examination of the bone forming token (Osteocalcin, alkaline phosphatase), and the dissolution of bone tissue (β-CrossLaps), Dual-energy X-ray densitometry (L1-L4). Results: Revealed a significant reduction in BMD females with MH as compared to control (Z-criterion –1.23 ± 0.73 and 0.34 ± 0.80, STD, respectively, p < 0.00001). BMD significant decrease (–1.5 STD) found a third of individuals with MH and not seen in the control group, p = 0.01. When assessing young BMD depending on the severity of MVP statistically reliable differences had been received. At the same time that boys with MVP in conjunction with signs MH is characterized by the lowest BMD values. In the analysis of laboratory parameters revealed a significant increase in Osteocalcin and alkaline phosphatase in the Group of persons with MH, indicating bone forming activity in these patients. At the same time, for people with MH is characterized by increasing the level of β-CrossLaps (marker of the dissolution of bone tissue). With the increased activity of the dissolution of bone tissue associated bone such signs as dolyhostenomelia, deformations of thorax and arachnodaktylia. Conclusion: bone signs dysembriogenesis involved in the diagnostic algorithm MH, contributes to the abnormal formation of bone tissue in these patients. For them is characterized by activation synthesis and dissolution of bone tissue, reducing BMD.

scholarly journals Effects of Aleurone Layer Extract From Black Rice (Oryza sativa L.) on Bone Mineral Density and Bone-Related Biomarkers of Ovariectomized Rats (FS14-05-19)

2019 ◽  
Vol 3 (Supplement_1) ◽  
Author(s):  
Sung Hyen Lee ◽  
Jung-Bong Kim ◽  
Hwan Hui Chang ◽  
Jeong Sook Choi ◽  
Gyeong Hwang ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Body Weight ◽  
Bone Mineral ◽  
Bone Health ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Aleurone Layer ◽  
Whole Grain ◽  
Black Rice ◽  
Mineral Density

Abstract Objectives Black rice contains functional compounds, including anthocyanins. In a previous study, black rice increased bone mineral density and strength in ovariectomized rats, although its biological effects on bone-related markers have not been evaluated. The aleurone layer of black rice (BRA) contains most of anthocyanins of black rice. This study examined the effects of BRA extract on bone mineral density and bone-related markers of ovariectomized rats. Methods We compared the anthocyanin amount between BRA and whole grain of black rice and evaluated whether BRA extract can effectively improve bone health in ovariectomized rats at 30 mg/kg body weight (BRA30) and 90 mg/kg body weight (BRA90). These amounts are approximately one seventh and one half of the level (200 mg/kg body weight) used in the previous experiments. We compared its effects on bone-related biomarkers with those of isoflavones, which have been used to improve bone health in postmenopausal women. Results The cyanidin 3-glucoside and peonidin-3-glucoside contents in BRA were 7.18 mg/g and 1.79 mg/g, respectively, which are 5.5-fold and 4.2-fold higher than those in the whole grain of black rice. Supplementation with BRA extract significantly decreased body weight compared with the control group of ovariectomized rats not treated with BRA extract. Mineral density of tibia was significantly higher in the BRA90 group than in the control group. The breaking force of tibia and femur and the concentrations of serum and bone calcium were higher in BRA30 and BRA90 than in the control. However, urinary pyridinoline level, serum alkaline phosphatase activity, and parathyroid hormone concentration decreased in the BRA groups compared to the control group. Conclusions The results suggest that BRA has more anthocyanin than whole grain black rice and can improve bone health by modulating bone metabolism and reducing postmenopausal osteoporosis. Furthermore, the BRA extract showed similar or even higher effect on bone-related biomarkers than isoflavones and hence can be used as a good food source to manage bone health of most postmenopausal women. Funding Sources PJ01327901, National Institute of Agricultural Sciences.

scholarly journals Serum Dickkopf-1 Level in Postmenopausal Females: Correlation with Bone Mineral Density and Serum Biochemical Markers

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Sahar Fathi Ahmed ◽  
Neveen Fouda ◽  
Amal Ahmed Abbas
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Mineral ◽  
Bone Densitometry ◽  
Biochemical Markers ◽  
Mineral Density ◽  
Group I ◽  
Calcium Phosphorus ◽  
Serum Biochemical ◽  
Dickkopf 1

Objective. To assess serum level of Dickkopf-1 in postmenopausal females and its correlation with bone mineral density and serum biochemical markers.Methods. Bone densitometry, serum Dickkopf-1, calcium, phosphorus, and alkaline phosphatase were done in sixty postmenopausal females. Patients were divided according toTscore into osteoporosis (group I), osteopenia (group II), and normal bone mineral density that served as controls.Results. There was highly significant increase in serum Dickkopf-1 levels in postmenopausal females with abnormalTscore versus controls (P<0.001). Serum DKK-1 levels correlated negatively with both lumbarTscore (r=-0.69,P<0.001) and femurTscore (r=-0.64,P<0.001) and correlated positively with duration of menopause (r=0.61,P<0.001), while there was no significant correlation between serum levels of either calcium, phosphorus or alkaline phosphatase, and both serum Dickkopf-1 levels and the level of bone mineral density (P>0.05).Conclusion. Postmenopausal females may suffer from osteoporosis as evidenced by bone densitometry. Postmenopausal women with significantly increased serum Dickkopf-1 had more significant osteoporosis. Prolonged duration of menopause and increased serum Dickkopf-1 are important risk factors for the development and severity of osteoporosis.

Effect of vitamin K2 and vitamin D3 on bone mineral density in children with acute lymphoblastic leukemia: a prospective cohort study

2021 ◽  
Vol 34 (4) ◽  
pp. 441-447
Author(s):  
Ismail Solmaz ◽  
Mehmet Akif Ozdemir ◽  
Ekrem Unal ◽  
Ummuhan Abdurrezzak ◽  
Sebahattin Muhtaroglu ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Acute Lymphoblastic Leukemia ◽  
Bone Mineral ◽  
Vitamin D3 ◽  
Lymphoblastic Leukemia ◽  
Vitamin K2 ◽  
Control Group ◽  
Study Group ◽  
Mineral Density

Abstract Objectives Current treatment protocols in acute lymphoblastic leukemia (ALL) are associated with high remission rates and long life expectancy, enhancing the importance of quality of life and prevention of treatment-related complications in patient care. As osteoporosis is a frequent complication in patients under chemotherapy, we investigated the effect of vitamin K2 (100 mcg menaquinone-7) and vitamin D3 (10 mcg calcitriol) on bone metabolism in children with ALL. Methods Twenty-nine consecutive patients recently diagnosed with B precursor ALL (B-ALL) and treated according to the Turkish Acute Lymphoblastic Leukemia Berlin Frankfurt Münster 2000 protocol were randomly assigned into study and control groups. The study group (n=15, M/F: 8/7, age 1–14.5 years, mean 6.5 years) received vitamin K2 and vitamin D3 with their chemotherapy, while the control group (n=14, M/F 9/5, age 2–17 years, mean 7.1 years) received chemotherapy only. Serum calcium, phosphorus, magnesium, alkaline phosphatase, bone-specific alkaline phosphatase, uncarboxylated osteocalcin (ucOC), tartrate resistant acid phosphatase 5b, carboxyl terminal procollagen propeptide (PICP), osteoprotegerin (OPG), and receptor activator nuclear kappa B ligand (RANKL) were measured and bone mineral density (BMD) was determined at baseline and first, second, third and sixth months. Results The study group had higher serum OPG/RANKL ratio and lower ucOC levels compared to the control group at the first month; PICP levels were higher in the study group at second and third months. Conclusions These results suggest an early beneficial effect of the combination of vitamin K2 and vitamin D3 on BMD in ALL patients especially during the period of intensive steroid therapy in the first months.

Downhill running increases bone mass accumulation and bone tissue morphology through TGF- β /Smad pathway in mice

2020 ◽  
Author(s):  
Xianghe Chen ◽  
Peng Sun ◽  
Aiguo Chen ◽  
Haixia Peng ◽  
Xianliang Zhang ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Mass ◽  
Bone Tissue ◽  
Bone Mineral ◽  
Osteoblastic Differentiation ◽  
Control Group ◽  
Mineral Density ◽  
Smad Pathway ◽  
Downhill Running

Abstract Background: Compelling evidence has demonstrated that impact exercise can help to accumulate more bone mass. However, the cellular mechanism is less clear. The current study aimed to examine the effects of downhill running on osteoblast formation and the ability of mineralization in mice. The expression of transforming growth factor-b (TGF-b)/Smad pathway molecules was also determined. Methods: Twenty-eight 4-week-old C57BL/6 female mice were randomly assigned to either downhill running group (DG) or control group (CG). DG mice received a daily forty-minute downhill training on a decline treadmill (9 o decline) for 8 weeks. Eight weeks later, all mice were killed. Bone marrow stromal cells were collected and cultured for assessment of osteoblastic differentiation and the capacity of osteoblastic mineralization. We determined the protein (Western Blot) and mRNA (RT-PCR) expression of TGF-b, p-Smad2/3/4, and Runx2 in bone tissue. The bone mineral density (BMD) and histomorphological changes were also examined. Results: Eight-week training significantly increased bone mineral density (BMD) and enhanced the expression of alkaline phosphatase positive (ALP + ) osteoblasts in tibia epiphysis. Downhill running also promoted bone volume fraction (BV/TV), trabecular number (Tb.N) and trabecular thickness (Tb.Th), while it decreased the trabecular separation (Tb.Sp). Additionally, the numbers of alkaline phosphatase positive colony forming units-fibroblastic (ALP ＋ CFU-f) cells and the area of mineralized nodule formation were significantly higher in training group compared with the control group. Furthermore, the mRNA expression of TGF-b, Smad2, Smad3, Smad4 and Runt-related transcription factor 2 (Runx2) were significantly elevated by downhill training, and the protein expression of p-Smad2, p-Smad3, p-Smad4 and Runx2 also increased in exercise group. Conclusion: The findings suggested that downhill running enhanced bone accrual in mice mainly by promoting osteoblastic differentiation and the ability of mineralization. The beneficial changes partly be regulated by TGF-b/Smad signaling pathway.

scholarly journals Pamidronate and zoledronate effects in the increment of bone mineral density and histomorphometry in rats

2011 ◽  
Vol 26 (2) ◽  
pp. 114-120
Author(s):  
Antônio Fiel Cruz Júnior ◽  
Carlos Buchpiguel ◽  
Roberto Guarniero ◽  
Antonio Barbieri
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Mineral ◽  
Young Female ◽  
Female Rats ◽  
Laboratory Evaluation ◽  
Control Group ◽  
Biochemical Evolution ◽  
Mineral Density ◽  
Total Proteins

PURPOSE: To compare increment of bone mineral density (BMD) with pamidronate, zoledronate and the isolated effect of proteinous diet in undernourished oophorectomized and non-oophorectomized female rats, besides validating BMD's indexes. METHODS: 60 young female Lewis rats were divided into five experimental groups and a control group, oophorectomized and non-oophorectomized. The administration of drugs were submitted to proteinous and aproteinous diets. The variables analyzed were weight, bone densitometry, histomorphometry and biochemical evolution. RESULTS: In weight evaluation, the first interval showed a statistically meaningful increase in oophorectomized sample. In densitometry evaluation, the first interval showed statistically meaningful decrease in four medicated groups and third showed a statistically meaningful increase in 2 non-oophorectomized groups. In laboratory evaluation, there were an increase of total proteins and globulin, decrease of alkaline phosphatase, phosphorus and calcium (except for the oophorectomized) in four medicated groups and increase of phosphorus and calcium in 2 not medicated groups. In histomorphometric evaluation, the oophorectomized groups had smaller increment of BMD. CONCLUSIONS: The pamidronate and zoledronate have shown effectives in the increment of BMD. The proteinous diet itself possesses therapeutic effect in BMD though not significant compared with medicated animals. The results of histomorphometry allow validating BMD's indexes in this experimental model.

Changes in bone metabolism associated with exposure to industrial vibration

2020 ◽  
pp. 766-766
Author(s):  
A. V. Sukhova ◽  
E. N. Kryuchkova
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Resorption ◽  
Bone Formation ◽  
Bone Metabolism ◽  
Biochemical Markers ◽  
Mineral Density ◽  
Local Vibration ◽  
Markers Of Bone Formation

The influence of general and local vibration on bone remodeling processes is investigated. The interrelations between the long - term exposure of industrial vibration and indicators of bone mineral density (T-and Z-criteria), biochemical markers of bone formation (osteocalcin, alkaline phosphatase) and bone resorption (ionized calcium, calcium/creatinine) were established.

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