scholarly journals Bone mineral density and bone metabolic markers in laboratory in men with marfanoid habitus

2017 ◽  
Vol 8 (6) ◽  
pp. 42-49
Author(s):  
Evgeny V. Timofeev ◽  
Tatiana I. Belousova ◽  
Elena V. Vutrih ◽  
Eduard V. Zemtsovsky ◽  
Andrey Yu. Olkhovik
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Tissue ◽  
Bone Mineral ◽  
Diagnostic Algorithm ◽  
Control Group ◽  
Mineral Density ◽  
Metabolic Markers ◽  
Power Shortages ◽  
Increased Activity

The aim of the research was the study of bone mineral density (BMD) and activity laboratory markers of bone metabolism in juniors with power shortages and marfanoid habitus (MH). Materials and methods: Twenty 119 males are underweight aged 18 to 25 years old (average age of 20.4 ± 1.5 years). All surveyed was conducted anthropometric, phenotypical, Echocardiography study to identify the mitral valve prolapse (MVP), laboratory examination of the bone forming token (Osteocalcin, alkaline phosphatase), and the dissolution of bone tissue (β-CrossLaps), Dual-energy X-ray densitometry (L1-L4). Results: Revealed a significant reduction in BMD females with MH as compared to control (Z-criterion –1.23 ± 0.73 and 0.34 ± 0.80, STD, respectively, p < 0.00001). BMD significant decrease (–1.5 STD) found a third of individuals with MH and not seen in the control group, p = 0.01. When assessing young BMD depending on the severity of MVP statistically reliable differences had been received. At the same time that boys with MVP in conjunction with signs MH is characterized by the lowest BMD values. In the analysis of laboratory parameters revealed a significant increase in Osteocalcin and alkaline phosphatase in the Group of persons with MH, indicating bone forming activity in these patients. At the same time, for people with MH is characterized by increasing the level of β-CrossLaps (marker of the dissolution of bone tissue). With the increased activity of the dissolution of bone tissue associated bone such signs as dolyhostenomelia, deformations of thorax and arachnodaktylia. Conclusion: bone signs dysembriogenesis involved in the diagnostic algorithm MH, contributes to the abnormal formation of bone tissue in these patients. For them is characterized by activation synthesis and dissolution of bone tissue, reducing BMD.

Downhill running increases bone mass accumulation and bone tissue morphology through TGF- β /Smad pathway in mice

2020 ◽  
Author(s):  
Xianghe Chen ◽  
Peng Sun ◽  
Aiguo Chen ◽  
Haixia Peng ◽  
Xianliang Zhang ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Mass ◽  
Bone Tissue ◽  
Bone Mineral ◽  
Osteoblastic Differentiation ◽  
Control Group ◽  
Mineral Density ◽  
Smad Pathway ◽  
Downhill Running

Abstract Background: Compelling evidence has demonstrated that impact exercise can help to accumulate more bone mass. However, the cellular mechanism is less clear. The current study aimed to examine the effects of downhill running on osteoblast formation and the ability of mineralization in mice. The expression of transforming growth factor-b (TGF-b)/Smad pathway molecules was also determined. Methods: Twenty-eight 4-week-old C57BL/6 female mice were randomly assigned to either downhill running group (DG) or control group (CG). DG mice received a daily forty-minute downhill training on a decline treadmill (9 o decline) for 8 weeks. Eight weeks later, all mice were killed. Bone marrow stromal cells were collected and cultured for assessment of osteoblastic differentiation and the capacity of osteoblastic mineralization. We determined the protein (Western Blot) and mRNA (RT-PCR) expression of TGF-b, p-Smad2/3/4, and Runx2 in bone tissue. The bone mineral density (BMD) and histomorphological changes were also examined. Results: Eight-week training significantly increased bone mineral density (BMD) and enhanced the expression of alkaline phosphatase positive (ALP + ) osteoblasts in tibia epiphysis. Downhill running also promoted bone volume fraction (BV/TV), trabecular number (Tb.N) and trabecular thickness (Tb.Th), while it decreased the trabecular separation (Tb.Sp). Additionally, the numbers of alkaline phosphatase positive colony forming units-fibroblastic (ALP ＋ CFU-f) cells and the area of mineralized nodule formation were significantly higher in training group compared with the control group. Furthermore, the mRNA expression of TGF-b, Smad2, Smad3, Smad4 and Runt-related transcription factor 2 (Runx2) were significantly elevated by downhill training, and the protein expression of p-Smad2, p-Smad3, p-Smad4 and Runx2 also increased in exercise group. Conclusion: The findings suggested that downhill running enhanced bone accrual in mice mainly by promoting osteoblastic differentiation and the ability of mineralization. The beneficial changes partly be regulated by TGF-b/Smad signaling pathway.

BONE MINERAL DENSITY OF THE RATS TIBIA AFTER MODELING OF ARTIFICIAL MENOPAUSE AND INFLUENCE OF WHOLE BODY VIBRATION

2021 ◽  
pp. 71-76
Author(s):  
N. М. Kostyshyn
Keyword(s):  
Bone Mineral Density ◽  
Bone Tissue ◽  
Bone Mineral ◽  
Whole Body Vibration ◽  
Whole Body ◽  
Control Group ◽  
Mineral Density ◽  
Body Vibration ◽  
Group I ◽  
Experimental Group

Early deficiency of female sex hormones provokes bone loss in various parts of the skeleton, which further requires therapeutic correction. However, it is known that vibrational oscillations are anabolic for the trabecular layer of bone, which have been studied in both animal models and humans under physiological norms. It is likely that this anabolic reaction does not occur evenly throughout the skeleton, on the one hand, due to the cushioning properties of muscle tissue, ligaments, joints, and resonance, on the other. The aim of the study is to assess the condition and mineral density of the tibia under the condition of artificial menopause and the influence of non-physiological whole body vibration (WBV) with acceleration 0,3 g. Material and methods. The experiments were performed on 54 adult female Wistar rats. Rats were divided into three groups: control group (n=18) – SHAM surgery, experimental group I (n=18) - rats with ovariectomy, experimental group II (n=18) - rats after ovariectomy + WBV. Rats of were subjected to total vibration with acceleration of 0,3 g for 30 minutes 5 days a week for 24 weeks. At the 8th, 16th and 24th week of the experiment CT-densitometry of the tibia was performed. Results. The greatest loss of bone tissue was observed in experimental group I at the 24th week of the experiment, which decreased by 23.5% compared with the control group. At the 8th week of the study, the rate did not change statistically, and at the 16th - decreased to 8.1%. In experimental group II, bone density did not decrease statistically at the 8th and 16th week of our study, however, at the 24th week decreased by -16% to the control group, and compared with group I, increased by +11,2 % on the 24th day. The cortical layer of the tibial bone tissue did not change statistically in the experimental groups in relation to the control group. Factors that can affect the anabolic response in the tissues of the musculoskeletal system are the parameters of vibration, in particular the frequency and level of vibration acceleration. In our experimental study, we observed a decrease in bone mineral density in rats with ovariectomy and compared with ovariectomized rats subjected to vibration. We observed a positive correlation between whole body vibration and bone mass increase, which may be due to the effect of increased mechanical stress on the skeleton. We also demonstrated the negative effect of time after ovariectomy on bone density in experimental rats. The use of vibration maintained normal bone mineral density for a long time, and until the 16th week of the experiment, but at the 24th week, rate decreased significantly compared with the control group, but was higher than in group I. Analysis of CT scans showed high values of mineral density in experimental group II (ovariectomy + WBV) compared with experimental group I. Conclusions. The obtained results allow us to conclude that the WBV inhibits the accelerated remodeling after ovariectomy and prevents the rapid loss of bone tissue for a long time. To this end, mechanical vibrations should be used in the early menopause to maintain normal bone mineral mass. This therapy can be used alone and as an adjunct to osteoporosis medication. Therefore, early detection of osteopenia and timely appropriate treatment is important, which, in addition to antiresorptive drug treatment, should include lifestyle changes and exercise.

scholarly journals The influence of prolactin on bone mineral density (BMD) and some biochemical markers of ovariectomized rats

2010 ◽  
Vol 55 (No. 2) ◽  
pp. 83-88
Author(s):  
B. Dolińska ◽  
A. Suszka-Świtek ◽  
S. Dragan ◽  
F. Ryszka ◽  
R. Kołacz ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Acid Phosphatase ◽  
Bone Mineral ◽  
Biochemical Markers ◽  
Ovariectomized Rats ◽  
Control Group ◽  
Osseous Tissue ◽  
Mineral Density ◽  
Healthy Group

A model of postmenopausal osteoporosis was used in the study. 107 days after ovariectomy the animals were subcutaneously applied 1.0 IU PRL/kg BM or 1.0 IU calcitonin/kg BM for 7 days in single doses. The application of the preparations started again 80 days after the first application. After 194 days the animals were narcotized, blood was collected, and lumbar (L2–L4) and femoral bones were prepared. The material obtained was subjected to densitometric analysis of bone mineral density (BMD). In ovariectomized rats the loss of osseous tissue was observed only in the lumbar bone. The application of prolactin to ovariectomized rats caused an increase in the mineral density of the lumbar bone up to a BMD value equal to that of the control group which had not been subjected to ovariectomy (control healthy group – SHAM). Similar results were obtained in the case of calcitonin. An increase in osteocalcin concentration and activity of isoenzyme alkaline phosphatase (BAL), with a decrease in the activity of isoenzyme acid phosphatase (TRAP) was observed in experimental groups compared to control ones.

scholarly journals Effects of High-Impact Weight-Bearing Exercise on Bone Mineral Density and Bone Metabolism in Middle-Aged Premenopausal Women: A Randomized Controlled Trial

2021 ◽  
Vol 11 (2) ◽  
pp. 846
Author(s):  
Sung-Woo Kim ◽  
Myong-Won Seo ◽  
Hyun-Chul Jung ◽  
Jong-Kook Song
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Weight Bearing ◽  
Premenopausal Women ◽  
High Impact ◽  
Control Group ◽  
Middle Aged ◽  
Mineral Density ◽  
Bone Metabolic Markers ◽  
Metabolic Markers

This study examined the effects of high-impact weight-bearing exercise on bone mineral density (BMD) and bone metabolic markers in middle-aged premenopausal women. Forty middle-aged premenopausal women were initially enrolled, but thirty-one participants (40.34 ± 3.69 years) completed in the study. The subjects were randomly divided into two groups including the high-impact weight-bearing exercise group (HWE, n = 14) and control group (CON, n = 17). The HWE group participated in the exercise for 50 min a day, three days per week for four months, while the CON group maintained their regular lifestyle. The HWE program included 10 different high-impact weight-bearing exercises such as jumping and running. BMD was measured using DXA (Hologic, QDR 4500W, Marlborough, MA, USA). The bone metabolic markers including serum 25-(OH) D, intact parathyroid hormone (PTH), osteoprotegerin (OPG), osteopontin (OPN), receptor activator of nuclear factor κB ligand (RANKL), osteocalcin (OC), C-terminal telopeptide of type 1 collagen (CTX), and calcium were analyzed. The results showed that the BMDs of femur, lumbar, and forearm did not significantly change during the intervention period in both the HWE and CON groups. A significant decrease in bone formation markers such as OC (F = 10.514, p = 0.003, ηp2 = 0.266) and an increase in bone resorption marker including CTX (F = 8.768, p = 0.006, ηp2 = 0.232) were found only in the CON group, while these values did not change in the HWE group. There was a significant increase in serum 25-(OH) D (F = 4.451, p = 0.044, ηp2 = 0.133) in the HWE group. Our findings suggest that four months of HWE is not sufficient to improve BMD and bone metabolic markers, but this impact exercise program may prevent the age-associated changes in bone turnover markers in middle-aged premenopausal women.

Effect of vitamin K2 and vitamin D3 on bone mineral density in children with acute lymphoblastic leukemia: a prospective cohort study

2021 ◽  
Vol 34 (4) ◽  
pp. 441-447
Author(s):  
Ismail Solmaz ◽  
Mehmet Akif Ozdemir ◽  
Ekrem Unal ◽  
Ummuhan Abdurrezzak ◽  
Sebahattin Muhtaroglu ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Acute Lymphoblastic Leukemia ◽  
Bone Mineral ◽  
Vitamin D3 ◽  
Lymphoblastic Leukemia ◽  
Vitamin K2 ◽  
Control Group ◽  
Study Group ◽  
Mineral Density

Abstract Objectives Current treatment protocols in acute lymphoblastic leukemia (ALL) are associated with high remission rates and long life expectancy, enhancing the importance of quality of life and prevention of treatment-related complications in patient care. As osteoporosis is a frequent complication in patients under chemotherapy, we investigated the effect of vitamin K2 (100 mcg menaquinone-7) and vitamin D3 (10 mcg calcitriol) on bone metabolism in children with ALL. Methods Twenty-nine consecutive patients recently diagnosed with B precursor ALL (B-ALL) and treated according to the Turkish Acute Lymphoblastic Leukemia Berlin Frankfurt Münster 2000 protocol were randomly assigned into study and control groups. The study group (n=15, M/F: 8/7, age 1–14.5 years, mean 6.5 years) received vitamin K2 and vitamin D3 with their chemotherapy, while the control group (n=14, M/F 9/5, age 2–17 years, mean 7.1 years) received chemotherapy only. Serum calcium, phosphorus, magnesium, alkaline phosphatase, bone-specific alkaline phosphatase, uncarboxylated osteocalcin (ucOC), tartrate resistant acid phosphatase 5b, carboxyl terminal procollagen propeptide (PICP), osteoprotegerin (OPG), and receptor activator nuclear kappa B ligand (RANKL) were measured and bone mineral density (BMD) was determined at baseline and first, second, third and sixth months. Results The study group had higher serum OPG/RANKL ratio and lower ucOC levels compared to the control group at the first month; PICP levels were higher in the study group at second and third months. Conclusions These results suggest an early beneficial effect of the combination of vitamin K2 and vitamin D3 on BMD in ALL patients especially during the period of intensive steroid therapy in the first months.

scholarly journals Pamidronate and zoledronate effects in the increment of bone mineral density and histomorphometry in rats

2011 ◽  
Vol 26 (2) ◽  
pp. 114-120
Author(s):  
Antônio Fiel Cruz Júnior ◽  
Carlos Buchpiguel ◽  
Roberto Guarniero ◽  
Antonio Barbieri
Keyword(s):  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Mineral ◽  
Young Female ◽  
Female Rats ◽  
Laboratory Evaluation ◽  
Control Group ◽  
Biochemical Evolution ◽  
Mineral Density ◽  
Total Proteins

PURPOSE: To compare increment of bone mineral density (BMD) with pamidronate, zoledronate and the isolated effect of proteinous diet in undernourished oophorectomized and non-oophorectomized female rats, besides validating BMD's indexes. METHODS: 60 young female Lewis rats were divided into five experimental groups and a control group, oophorectomized and non-oophorectomized. The administration of drugs were submitted to proteinous and aproteinous diets. The variables analyzed were weight, bone densitometry, histomorphometry and biochemical evolution. RESULTS: In weight evaluation, the first interval showed a statistically meaningful increase in oophorectomized sample. In densitometry evaluation, the first interval showed statistically meaningful decrease in four medicated groups and third showed a statistically meaningful increase in 2 non-oophorectomized groups. In laboratory evaluation, there were an increase of total proteins and globulin, decrease of alkaline phosphatase, phosphorus and calcium (except for the oophorectomized) in four medicated groups and increase of phosphorus and calcium in 2 not medicated groups. In histomorphometric evaluation, the oophorectomized groups had smaller increment of BMD. CONCLUSIONS: The pamidronate and zoledronate have shown effectives in the increment of BMD. The proteinous diet itself possesses therapeutic effect in BMD though not significant compared with medicated animals. The results of histomorphometry allow validating BMD's indexes in this experimental model.

Relationships between serum leptin level and regional bone mineral density, bone metabolic markers in healthy women

2000 ◽  
Vol 79 (12) ◽  
pp. 1060-1064 ◽  
Author(s):  
Ichiro Iwamoto ◽  
Tsutomu Douchi ◽  
Shoichiro Kosha ◽  
Masato Murakami ◽  
Toshinori Fujino ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Mineral Density ◽  
Bone Metabolic Markers ◽  
Metabolic Markers ◽  
Serum Leptin ◽  
Healthy Women ◽  
Regional Bone Mineral Density

Effects of One-Year Adjuvant Treatment with Tamoxifen on Bone Mineral Density in Postmenopausal Breast Cancer Women

1996 ◽  
Vol 82 (1) ◽  
pp. 65-67 ◽  
Author(s):  
Sandro Barni ◽  
Paolo Lissoni ◽  
Gabriele Tancini ◽  
Antonio Ardizzoia ◽  
Marina Cazzaniga
Keyword(s):  
Breast Cancer ◽  
Bone Mineral Density ◽  
Alkaline Phosphatase ◽  
Bone Mineral Content ◽  
Bone Mineral ◽  
Adjuvant Treatment ◽  
Mineral Content ◽  
Postmenopausal Breast Cancer ◽  
Mineral Density ◽  
One Year

In this study, the authors have analyzed the possible effects of one-year adjuvant treatment with tamoxifen on bone mineral density in postmenopausal breast cancer women. Bone mineral content was studied by photon absorptiometry (I-125), whereas bone balance was analyzed indirectly by serum PTH, osteocalcin, calcitonin, calcium and alkaline phosphatase levels. Bone mineral content and serum bone-related substances were measured before starting treatment and after one year. Results were analyzed using Student's t test for paired data. No difference was found between the two measurements for bone mineral content, PTH, calcitonin, calcium and alkaline phosphatase levels. Measurements at entry and after one year of treatment showed a statistically significant difference ( P < 0.001) only for osteocalcin. In accordance with other authors, we can conclude that treatment with tamoxifen does not cause an increase in menopausal bone resorption. The finding that osteocalcin levels decreased after one year of therapy with tamoxifen is interesting, but further studies are necessary to clarify the role of such levels in predicting a turnover of bone balance towards osteoblastic activity.

scholarly journals Effect of Denosumab on Bone Mineral Density and Markers of Bone Turnover among Postmenopausal Women with Osteoporosis

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
A. Sánchez ◽  
L. R. Brun ◽  
H. Salerni ◽  
P. R. Costanzo ◽  
D. González ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Turnover ◽  
Postmenopausal Women ◽  
Bone Mineral ◽  
Bone Turnover Markers ◽  
Control Group ◽  
Bone Resorption Marker ◽  
Similar Response ◽  
Mineral Density ◽  
Turnover Markers

The aim of this study was to evaluate the effect of denosumab (Dmab) on bone mineral density (BMD) and bone turnover markers after 1 year of treatment. Additionally, the effect of Dmab in bisphosphonate-naïve patients (BP-naïve) compared to patients previously treated with bisphosphonates (BP-prior) was analyzed. This retrospective study included 425 postmenopausal women treated with Dmab for 1 year in clinical practice conditions in specialized centers from Argentina. Participants were also divided according to previous bisphosphonate treatment into BP-naïve and BP-prior. A control group of patients treated with BP not switched to Dmab matched by sex, age, and body mass index was used. Data are expressed as mean ± SEM. After 1 year of treatment with Dmab the bone formation markers total alkaline phosphatase and osteocalcin were significantly decreased (23.36% and 43.97%, resp.), as was the bone resorption marker s-CTX (69.61%). Significant increases in BMD were observed at the lumbar spine, femoral neck, and total hip without differences between BP-naïve and BP-prior. A better BMD response was found in BP-prior group compared with BP treated patients not switched to Dmab.Conclusion. Dmab treatment increased BMD and decreased bone turnover markers in the whole group, with similar response in BP-naïve and BP-prior patients. A better BMD response in BP-prior patients versus BP treated patients not switched to Dmab was observed.

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