scholarly journals Effects of blackcurrant and apple mash blending on the phenolics contents, antioxidant capacity, and colour of juices

2009 ◽  
Vol 27 (No. 5) ◽  
pp. 338-351 ◽  
Author(s):  
J. Oszmiański ◽  
A. Wojdyło
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Antioxidant Capacity ◽  
Apple Juice ◽  
Slight Reduction ◽  
Sample Storage ◽  
Antioxidant Power ◽  
Ferric Reducing Antioxidant Power ◽  
And Storage ◽  
Anthocyanin Degradation

The objective of this research was to evaluate the effect of blackcurrant mash blended with apple pulp during juice production and storage on its phenolic composition, antioxidant activity, L-ascorbic acid, and colour. Five variants of samples were prepared: apple juices from two cultivars: the Shampion and Idared cultivars without and with 20% of blackcurrant pulp and blackcurrant juice which were stored at 4°C and 30°C for 6 months. The apple juices prepared from the Idared and Shampion cultivars had a very low L-ascorbic acid contents (1.32 mg/l and 6.26 mg/l, respectively) whereas blackcurrant juice showed the highest amount of L-ascorbic acid, i.e. 704.3 mg/l. The addition of 20% of blackcurrant pulp before apple crashing resulted in a great difference between L-ascorbic acid contents in juices. The addition of blackcurrant fruits before apple crushing had a statistically significantly different (<i>P</i> < 0.05) influence on phenolic compounds, especially in Idared blended pulp. As compared with the control samples, flavan-3-ol concentration increased 4 times in juices made from 80% of Idared apples blended with 20% of blackcurrant fruits. Apple pulp blended with blackcurrant was richer in hydroxycinnamic acids (especially caffeic, <i>p</i>-coumaric, and neochlorogenic acids) than juices made only from apples. The results ranged from 83.05 to 3297.6µM T/100 ml for DPPH (1,1-diphenyl-2-picrylhydrazyl radical), from 20.64 to 490.93µM T/100 ml for ABTS (2,2’azinobis-(3-ethylbenzthiazoline-6-sulphonic acid)), and from 1.52 to 37.35µM T/ml for FRAP (Ferric reducing antioxidant power assay) for apple juice made from the Idared cultivar and for blackcurrant juice, respectively. The highest level of the antioxidant capacity (<i>P</i> < 0.05) observed in the blackcurrant sample was due to the effect of the high anthocyanin and ascorbic acid contents. The apple juice colour showed a moderate degradation with time as indicated by the slight reduction of <i>L</i>* values in the samples stored at 4°C for 6 months, and a much higher decrease of <i>L</i>* values in the samples stored at 30°C. The lightness of the apple blended with blackcurrant increased during storage as a result of the coloured anthocyanin degradation. The temperature during the sample storage (30°C) had a significant influence, resulting in a higher degradation of all phenolics compounds analysed, colour and antioxidant activity.

scholarly journals Chemical Characterization and Antioxidant Activity of Cedrus atlantica Manetti Tar (Atlas Cedar Tar)

2020 ◽  
Vol 8 (2) ◽  
pp. 244-255
Author(s):  
Inssaf Skanderi ◽  
Ourida Chouitah
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Condensed Tannins ◽  
Chemical Characterization ◽  
Antioxidant Power ◽  
Cedrus Atlantica ◽  
Total Antioxidant ◽  
Ferric Reducing Antioxidant Power ◽  
Total Polyphenolic Content

Cedrus atlantica Manetti Tar was analyzed by gas chromatography coupled to mass spectrometry, it was subjected to analyzes to know their total polyphenolic and condensed tannins contents, also their Ferric-reducing antioxidant power and Total antioxidant capacity. Chemical characterization identified 88 constituents where Himachalene and α-atlantone isomers (14.51 % - 4.07 %), Calacorene (3.52 %) and ar-Turmerone 3.35 %, were the major components, the total polyphenolic content and condensed tannins contents were 57.15 ± 0.15 milligrams equivalent of gallic acid /g tar and 4.41 ± 0.05 milligrams equivalent of catechin /g tar respectively .This extract showed remarkable Ferric-reducing antioxidant power with effective concentration equal to 50 ± 0.075 mg /mL ± 0,00028 and total antioxidant capacity equal to 262.75 mg equivalents of ascorbic acid /g tar ± 14,43. The experimental results indicated that our tar has promotive antioxidant activity.

scholarly journals Ekstraksi dan Bioaktivitas Antioksidan Pigmen Fikoeritrin dari Rumput Laut Merah Halymenia sp.

2020 ◽  
Vol 15 (2) ◽  
pp. 115
Author(s):  
Tias Suci Lailani ◽  
Ifah Munifah ◽  
Hermanto Hermanto
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Phosphate Buffer ◽  
Extraction Time ◽  
Red Seaweed ◽  
Antioxidant Power ◽  
Extract Yield ◽  
Ferric Reducing Antioxidant Power

Fikoeritrin adalah pigmen dari rumput laut merah Halymenia sp. dengan aktivitas antioksidan yang potensial untuk dikembangkan dalam bidang nutraseutikal. Ekstraksi fikoeritrin dapat dilakuan dengan berbagai jenis pelarut. Namun, penggunaan pelarut yang tidak sesuai dapat berdampak terhadap rendemen, kemurnian, dan juga bioaktivitas ekstrak fikoeritrin. Oleh karena itu, penelitian ini bertujuan untuk mendapatkan jenis pelarut dan durasi waktu ekstraksi (secara maserasi) yang tepat sehingga menghasilkan ekstrak dengan rendemen, kemurnian, dan daya antioksidan fikoeritrin terbaik. Ekstraksi dilakukan menggunakan tiga jenis pelarut (akuabides; buffer fosfat pH 6,8; dan aseton 80%) dengan variasi waktu maserasi (24, 48, 72, dan 96 jam). Spektrofotometri Ultraviolet-Visibel (UV-Vis) dipergunakan dalam identifikasi fikoeritrin, sementara Ferric Reducing Antioxidant Power (FRAP) merupakan metode dalam telaah daya antioksidan ekstrak. Hasil penelitian menunjukkan bahwa terdapat perbedaan jumlah rendemen ekstrak (w/w segar) dari ketiga pelarut, yaitu 1,76% (akuabides); 0,91% (aseton 80%); dan 2,91% (buffer fosfat pH 6,8). Selain itu, waktu ekstraksi juga menyebabkan perbedaan jumlah rendemen, yaitu 1,72% (24 jam); 1,51% mg/mL (48 jam); 1,37% (72 jam); dan 1,43% (96 jam). Hasil ini menunjukkan bahwa pelarut buffer fosfat dengan durasi waktu maserasi 24 jam adalah metode yang terbaik untuk mendapatkan rendemen fikoeritrin tertinggi dari rumput laut merah Halymenia sp. Aktivitas antioksidan dari ekstrak buffer fosfat ini terdeteksi sebesar 3,14 mg ekuivalen asam askorbat/100 g ekstrak pada konsentrasi 10 mg/mL. ABSTRACTPhycoerythrin is a pigment from red seaweed Halymenia sp., with antioxidant activity which is prospective in nutraceutical development. There are several solvents that could be used in the extraction of phycoerythrin. However, the use of inappropriate solvents may impact the yield, purity, and bioactivity of phycoerythrin extract. Therefore, this study aimed to obtain the type of solvent and the duration of extraction (with maceration technique) to produce the highest yield, purity, and antioxidant activity of phycoerythrin extract. Extraction was carried out using three types of solvents (aquabidest, phosphate buffer pH 6.8, and acetone 80%) with variations in extraction time (24, 48, 72, and 96 hours). Spectrophotometric Ultraviolet-Visible (UV-Vis) was used to identify the phycoerythrin, and Ferric Reducing Antioxidant Power (FRAP) was applied to analyze the antioxidant activity in the extract. The results showed that there were differences in the amount of extract yield (w/w fresh) from three solvents, which was 1.77% (aquabidest), 0.91% (acetone 80%), and 2.91% (phosphate buffer pH 6.8). Moreover, extraction duration was also affecting the amount of yield, which was1.78% (24 hours), 1.51% mg/mL (48 hours), 1.37% (72 hours), and 1.43% (96 hours). These results indicated that the phosphate buffer solvent with a 24-hour extraction duration was the best method to get the highest yield of phycoerythrin from the red seaweed Halymenia sp. The antioxidant activity from the phosphate buffer extract was detected up to 3.14 mg equivalent ascorbic acid /100 g extract at a concentration of 10 mg/mL.

scholarly journals Research of physicochemical properties and antioxidant activity of beetroots as affected by vacuum microwave drying conditions

2021 ◽  
Vol 5 (3(61)) ◽  
pp. 40-45
Author(s):  
Yan Liu ◽  
Sergei Sabadash ◽  
Zhenhua Duan
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Physicochemical Properties ◽  
Microwave Power ◽  
Sample Thickness ◽  
Microwave Drying ◽  
Antioxidant Power ◽  
Vacuum Degree ◽  
Vacuum Microwave ◽  
Ferric Reducing Antioxidant Power

The object of research is the beetroots, dried by vacuum microwave drying at different conditions. Physicochemical properties and antioxidant activity of beetroots were studied using vacuum microwave drying at different microwave power (500, 1000, and 1500 W), vacuum degree (–0.05, –0.07, and –0.09 MPa) and sample thickness (2, 4, and 6 mm). A colorimeter was used to evaluate the color quality of beetroots. Colorimetric methods were used to determinate contents of betalain, ascorbic acid and total flavonoid, and antioxidant activity (ferric reducing antioxidant power assay) of beetroots. Results showed that the drying time decreased with increasing microwave power and vacuum degree, while increased significantly with the increase of sample thickness. The lightness (L*) of dried beetroots was higher than that of fresh beetroots. The values of redness (a*) increased with the increase of vacuum degree. The values of yellowness (b*) increased with the growth of vacuum degree and microwave power, while reduced as the sample thickness added. The total color difference (∆E) of dried beetroots reduced with increasing vacuum degree, and displayed the lowest value (5.95) at a vacuum degree of –0.09 MPa as compared to fresh beetroots. The content of betacyanin, betaxanthin and ascorbic acid displayed a declining tendency with the growth of microwave power, while increased with the increase of vacuum degree. And the total flavonoid content of beetroots illustrated a decreasing tendency with the increase of vacuum degree, microwave power and sample thickness. The ferric reducing antioxidant power (FRAP) of dried beetroots decreased significantly with the increase of microwave power, and showed the highest value (14.70 mg trolox equivalents/g) at a microwave power of 500 W. The most favorable conditions for vacuum microwave drying of beetroots were microwave power of 500 W, vacuum degree of –0.09 MPa and sample thickness of 2 mm. It leads to better physicochemical properties of bioactive compounds and higher antioxidant activity of dried beetroots. The dried beetroots can be used as functional foods and value-added food products.

scholarly journals Antioxidants: Terminology, Methods, and Future Considerations

Antioxidants ◽  
2019 ◽  
Vol 8 (8) ◽  
pp. 297 ◽  
Author(s):  
Khiena Brainina ◽  
Natalia Stozhko ◽  
Marina Vidrevich
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Activity Concentration ◽  
Antiradical Activity ◽  
Antioxidative Activity ◽  
Antioxidant Power ◽  
Trolox Equivalent ◽  
Ferric Reducing Antioxidant Power ◽  
Quality Diet

Unreliable terminology and incompatible units of antioxidant activity/concentration expression lead to the failure of antioxidant clinical trials, ambiguity of conclusions about the effect of a chosen therapy in medicine and evaluation of food quality, diet, difficulties using information in monitoring the training process in sports, etc. Many different terms (antiradical activity, antioxidant activity, antioxidant capacity, antioxidant power, antioxidant ability) and methods: Trolox equivalent capacity assay (TEAC), Ferric Reducing Antioxidant Power assay (FRAP), Cupric Reducing Antioxidant Capacity assay (CUPRAC), antioxidative activity assay (ABTS), the oxygen radical absorbance capacity (ORAC), and different options of electrochemical ones) proposed for the determination of antioxidants are described. Possible approaches to the development of this field of science and practice are considered.

scholarly journals Effect of flavonoids content on antioxidant activity of commercial cosmetic plant extracts

2013 ◽  
Vol 59 (3) ◽  
pp. 63-75 ◽  
Author(s):  
Paulina Malinowska
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Plant Extracts ◽  
Natural Antioxidants ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Antioxidant Power ◽  
Trolox Equivalent ◽  
Ferric Reducing Antioxidant Power ◽  
The Relationship ◽  
Herb Extracts

Abstract The aim of this work was to evaluate the antioxidant capacity and flavonoids content in 10 commercial cosmetic plant extracts used in cosmetics industry. Antioxidant activity of plant extracts were measured using two methods: FRAP (Ferric Reducing Antioxidant Power) and TEAC (Trolox Equivalent Antioxidant Capacity). The relationship between flavonoids content and antioxidant capacity of plant extracts were checked. As a result of this research it was found that FRAP and TEAC values of plant extracts significantly depend on the flavonoids content. The highest antioxidant activity, both in FRAP and TEAC assays, was observed for arnica flowers, hawthorn flowers and lungwort herb extracts. These extracts can be used as source of natural antioxidants for the prolongation of the oxidative stability of cosmetic products. Additionally, they can replace synthetic antioxidants

scholarly journals Examining the Performance of Two Extraction Solvent Systems on Phenolic Constituents from U.S. Southeastern Blackberries

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 4001
Author(s):  
Xiaoxi Liao ◽  
Phillip Greenspan ◽  
Ronald B. Pegg
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Total Phenolics ◽  
Antioxidant Properties ◽  
Solvent System ◽  
Antioxidant Power ◽  
Extraction Solvent ◽  
Phenolic Constituents ◽  
Ferric Reducing Antioxidant Power ◽  
Solvent Systems

Two common extraction solvent systems, namely acidified aqueous methanol and acidified aqueous acetone, were used to extract blackberry phenolics, and the antioxidant properties of the recovered extracts were compared. The crude extracts were fractionated into low- and high-molecular-weight phenolics by Sephadex LH-20 column chromatography. The hydrophilic-oxygen radical absorbance capacity (H-ORACFL), ferric reducing antioxidant power (FRAP), and the cellular antioxidant activity (CAA) assays were employed as indices to assess antioxidant capacity of the extracts and their respective fractions. The methanolic solvent system displayed a greater efficiency at extracting anthocyanin and flavonol constituents from the blackberries, while the acetonic solvent system was better at extracting flavan-3-ols and tannins. Anthocyanins were the dominant phenolic class found in the blackberries with 138.7 ± 9.8 mg C3G eq./100 g f.w. when using methanol as the extractant and 114.6 ± 3.4 mg C3G eq./100 g f.w. when using acetone. In terms of overall antioxidant capacity of blackberry phenolics, the acetonic solvent system was superior. Though present only as a small percentage of the total phenolics in each crude extract, the flavan-3-ols (42.37 ± 2.44 and 51.44 ± 3.15 mg/100 g f.w. in MLF and ALF, respectively) and ellagitannins (5.15 ± 0.78 and 9.31 ± 0.63 mg/100 g f.w. in MHF and AHF, respectively) appear to account for the differences in the observed antioxidant activity between the two solvent systems.

scholarly journals Microorganisms and Biostimulants Impact on the Antioxidant Activity of Buckwheat (Fagopyrum esculentum Moench) Sprouts

Antioxidants ◽  
2020 ◽  
Vol 9 (7) ◽  
pp. 584 ◽  
Author(s):  
Robert Witkowicz ◽  
Wioletta Biel ◽  
Edyta Skrzypek ◽  
Joanna Chłopicka ◽  
Katarzyna Gleń-Karolczyk ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Water Soluble ◽  
Strongly Correlated ◽  
Growth Promoters ◽  
Pythium Oligandrum ◽  
Antioxidant Power ◽  
Plant Growth Promoters ◽  
Ferric Reducing Antioxidant Power ◽  
Fagopyrum Esculentum Moench

The study analyzes the influence of plant growth promoters and biological control agents on the chemical composition and antioxidant activity (AA) in the sprouts of buckwheat. The AA of cv. Kora sprouts was higher than cv. Panda, with 110.0 µM Fe2+/g (FRAP—Ferric Reducing Antioxidant Power), 52.94 µM TRX (Trolox)/g (DPPH—1,1-diphenyl-2-picrylhydrazyl), 182.7 µM AAE (Ascorbic Acid Equivalent)/g (Photochemiluminescence—PCL—ACW—Water-Soluble Antioxidant Capacity) and 1.250 µM TRX/g (PCL—ACL—Lipid-Soluble Antioxidant Capacity). The highest AA was found in the sprouts grown from seeds soaked in Ecklonia maxima extract and Pythium oligandrum (121.31 µM Fe2+/g (FRAP), 56.33 µM TRX/g (DPPH), 195.6 µM AAE/g (PCL—ACW) and 1.568 µM TRX/g (PCL—ACL). These values show that the antioxidant potential of buckwheat sprouts is essentially due to the predominant hydrophilic fraction of antioxidants. The AA of the sprouts was strongly correlated with total polyphenol content.

scholarly journals EFFECT OF OVEN AND MICROWAVE DRYING ON POLYPHENOLS CONTENT AND ANTIOXIDANT CAPACITY OF HERBAL TEA FROM STROBILANTHES CRISPUS LEAVES

2018 ◽  
Vol 11 (6) ◽  
pp. 363 ◽  
Author(s):  
Nur Fatimah Lasano ◽  
Asmah Rahmat ◽  
Nurul Shazini Ramli ◽  
Mohd Fadzelly Abu Bakar
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Radical Scavenging ◽  
Tea Plant ◽  
Microwave Drying ◽  
Total Phenolic ◽  
Flavonoid Content ◽  
Antioxidant Power ◽  
Strobilanthes Crispus ◽  
Ferric Reducing Antioxidant Power

Objective: This study aimed to evaluate the effect of oven and microwave drying on total phenolic content (TPC), total flavonoid content (TFC), and antioxidant capacity of unfermented and fermented tea developed from Strobilanthes crispus leaves.Methods: TPC and TFC were estimated using a spectrophotometric method, while antioxidant capacity was determined using ferric reducing antioxidant power assay and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assay. Camellia sinensis (tea plant), that is, used for the production of all varieties of commercial tea and fresh S. crispus leaves were served as controls in this study.Results: The highest antioxidant activity and TPC were observed in S. crispus tea developed from microwave-dried leaves, while the highest TFC was observed in oven-dried tea. Unfermented S. crispus tea showed significantly higher values (p<0.05) for antioxidant activity, TPC, and TFC as compared to fermented S. crispus tea. A strong and moderate correlation was observed between antioxidant activity and TPC as well as TFC values.Conclusion: The present work clearly showed that S. crispus tea developed from microwave-dried leaves able to preserve the polyphenols and hence contribute to excellent antioxidant capacity. Incorporation of unfermented S. crispus tea in the diet can be a good source of natural antioxidant.

scholarly journals Antioxidant Activity of Fruits Produced in Northern Greece

HortScience ◽  
2010 ◽  
Vol 45 (9) ◽  
pp. 1341-1344 ◽  
Author(s):  
Antonios Petridis ◽  
Magdalene Koukourikou ◽  
Thomas Sotiropoulos ◽  
Dimitrios Stylianidis
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
High Activity ◽  
Antioxidant Activities ◽  
Fresh Weight ◽  
Northern Greece ◽  
Frap Assay ◽  
Antioxidant Power ◽  
Cornelian Cherry ◽  
Ferric Reducing Antioxidant Power

The antioxidant activities (μmol ascorbic acid equivalent/g fresh weight) of the edible part of fruits grown in northern Greece were determined using the ferric-reducing antioxidant power (FRAP) assay. Differences were observed among species as well as cultivars of the same species. Cornelian cherry had the highest FRAP value followed by jujube. Cherries, black grapes, and blackberry showed high activity followed by pears, persimmons, plums, peaches, white grapes, pomegranates, apples, nectarines, kiwifruits, quinces, figs, and apricots.

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