scholarly journals The effect of selected factors on the content of protein and nitrates in potato tubers

2011 ◽  
Vol 51 (No. 10) ◽  
pp. 431-438 ◽  
Author(s):  
J. Lachman ◽  
K. Hamouz ◽  
P. Dvořák ◽  
M. Orsák

The aim of the study was to determine the effect of region, variety, year and ecological and conventional way of cultivation on the content of total protein and nitrates in potato tubers in three-year field trials. The effects of selected factors on nitrate and protein contents were similar, more significant for nitrates. Growing regions, varieties and years of cultivation caused statistically significant effects whereas the effect of ecological way of cultivation was less pronounced and showed a tendency without significance. Higher region showed lesser average nitrate (113.4 mg/kg) and protein content (4.27% dry matter) in comparison with lower region (143.7 mg/kg and 4.72% dry matter). The highest nitrate content was found in Impala and Karin varieties (199.2 and 167.6 mg/kg); Karin variety was also rich in total proteins (4.86% dry matter). Ecological way of cultivation showed no significant difference on nitrate content (136.9 mg/kg) and protein content (4.41%) as compared with conventional way of cultivation (153.9 mg/kg, 4.32%).

2011 ◽  
Vol 51 (No. 11) ◽  
pp. 517-522
Author(s):  
K. Zarzecka ◽  
M. Gugała

Field experiments were carried out at the Agricultural Experimental Nuisance Station of University of Podlasie in Siedlce in the years 1999–2001. The influence of herbicides and their mixtures on total proteins content and on proper proteins in eatable potato tubers were analysed. The weeding chemical agents (pesticides) used in the potato treatment increased the total protein content by 0.09–0.18% and proper proteins content by 0.15–0.34% in the tubers in contrast with the ones of the control object. The statistical analysis also revealed the importance of the cultivar and weather conditions during the experimental years.


Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1106
Author(s):  
Krystyna Zarzecka ◽  
Marek Gugała ◽  
Iwona Mystkowska ◽  
Anna Sikorska

The aim of the study was to determine the effect of herbicides and herbicides used with biostimulants on the content of total and true protein in potato tubers. The three-year field experiment was carried out using the random two-way split-plot arrangement in three repetitions. The examined factors were: I—three cultivars of potato: Bartek, Gawin, Honorata; II—five method applications of herbicides and herbicides with biostimulants: 1. Control—mechanical weeding, 2. Harrier 295 ZC (linuron + clomazone), 3. Harrier 295 ZC + Kelpak SL (linuron + clomazone and extract from algae Ecklonia maxima), 4. Sencor 70 WG (metribuzin), 5. Sencor 70 WG + Asahi SL (metribuzin and sodium p-nitrophenolate, sodium o-nitrophenolate, sodium 5-nitroguolacolate). On the plots sprayed with herbicides and herbicides mixed with biostimulants, a significant increase in the content of total and true proteins and the share of true protein in total protein in relation to tubers harvested from the control object was found. Genetic features of cultivars determined the content of total and true protein in potato tubers. The highest total and true protein was accumulated by tubers of the Bartek cultivar, and the least by the Honorata cultivar.


2011 ◽  
Vol 65 (5-6) ◽  
pp. 385-397
Author(s):  
Nadezda Prica ◽  
Milan Baltic ◽  
Milica Zivkov-Balos ◽  
Zeljko Cupic ◽  
Zeljko Mihaljev

Meat products play an important role in the modern diet. The production of cooked sausages comprises an important segment in the complex meat industry. Hot dogs are prepared according to production specifications and differences in the quality of products can therefore be expected. This paper presents a comparative analysis of chemical parameters in products of five different manufacturers at the Novi Sad market. Water content, fat, protein, sodium chloride, the relative protein content of connective tissue in meat protein (RCP) and ash were analyzed. The difference between the average contents of chemical quality parameters in hot dogs was examined. The average water content ranged from 56.29?3.30% to 61.44?4.08%. The average total fat content ranged from 17.60?3.07% to 20.66?4.65%. No statistically significant difference (p?0.05) was detected in the fat content of the products of manufacturers D and B, as well as C and B. Between the average fat content of the producers E and D the difference in fat content was found to be p?0.01. In all other cases, the difference between the average fat content was p?0.001. The average total protein content in hot dogs ranged from 11.77?1.08% and 15.41?1.82. No statistically significant difference (p?0.05) was detected between the average total protein content in hot dogs of manufacturers A and C. Between the average protein content of D and E meat manufacturers the statistically significant difference had a value of p?0.01, and the average protein content in hot dogs of all other manufacturers had a significant difference (p?0.001). The total protein content in all five manufacturers of hot dogs met the prescribed standard (10%). RCP content in the hot dogs of different producers ranged from 17.34?0.95% to 22.33?2.08%. No statistically significant difference (p?0.05) was detected in the average contents of RCP in hot dogs of manufacturers D and E and A and B. but a statistically significant difference was detected (p?0.001). between the average content of RCP in all other manufacturers of hot dogs. The average chloride content in hot dogs from different manufacturers ranged from 1.30?0.10% to 2.55%?0.16%. The average ash content in different hot dog producers ranged from 1.99?0.18% to 3.46?0.22%. The average content of chloride and ash in all five manufacturers was significantly different.


2019 ◽  
Vol 34 (4) ◽  
pp. 867-872
Author(s):  
Majlinda Ademi ◽  
Icko Gjorgoski ◽  
Ilbert Ademi

Alkaline water is in the focus of scientific interest over the last decade, due to its ability to alkalize the organism as well as its antioxidant effect. The aim of our research is to determine the impact of ionized water by adding enzymatic and non-enzymatic antioxidants, glutathione and vitamin C, during hyperthermic stress, on the concentration of total proteins. The experiment performed on a white laboratory Wistar rats, a female sex, weighing 180-220 g, young rats, divided into three groups of 15 individuals. Oxidative stress was caused by acute hyperthermic exposure at 41˚C. The first group is the control group (KPM), the second group is treated with ionized water (TAM), and the third group with ionized water with added glutathione and vitamin C (TAD). The duration of treatment lasted 21 days. Acute hyperthermic exposure caused a significant difference in total protein concentration between the 7th and 14th day taken as the starting points and the 21st day of treatment, respectively, on the day of hyperthermic exposure of rats. Such a significant difference was observed in the KPM and the TAM group (p <0.001), whereas the TAD group is an exception where no statistically significant difference was observed in the same period (p> 0.05). Animals treated with ionized water on day 14 showed a significantly higher total protein concentration (p <0.01) compared to the group receiving only natural water, which is consistent with the finding obtained by comparison between the KPM and TAD group at 21st day where the difference is also statistically significant (p <0.05). The acute application of the treatment with ionized water, without or in combination with other antioxidants, in the absence of hyperthermic stress does not lead to a significant alteration in protein concentration. The hyperthermic exposure intensifies the production of oxygen radicals and the potential for oxidative modification of proteins and molecules with a protein structure. Individual action of ionized water as well as synergism with the added antioxidants caused a high protective effect on oxidative damage. Because of this, the effect of oxidative cleavage is less pronounced in rats treated with ionized water and antioxidants because they have a stronger antioxidant defense that prevents oxidative modification of proteins and all biomolecules in the body.


2002 ◽  
Vol 42 (5) ◽  
pp. 541 ◽  
Author(s):  
J. L. Jacobs ◽  
F. R. McKenzie ◽  
G. A. Kearney

A study determined the effects of differing rates of nitrogen fertiliser [0 (N0), 25 (N1), 50 (N2) and 75�kg N/ha (N3)] during late autumn (T1) and mid- (T2) and late (T3) winter on the nutritive characteristics of perennial ryegrass over a 28-day period after each application. All nitrogen applications were made to pastures with a post-grazed residual mass (dry matter) of 1400 kg/ha. Changes in metabolisable energy followed similar patterns for all treatments within a given period. Metabolisable energy was highest in T1, ranging from 11.8 to 13.1 MJ/kg dry matter, followed by T2 (11.5-12.3 MJ/kg dry matter) and T3 (10.6-11.5 MJ/kg dry matter). Changes in crude protein for all treatments at each application time were similar, irrespective of rate of nitrogen application. At the commencement of treatment application times, the existing crude protein content (%DM) was highest in N3 (T1�19, T2 23, T3 22), followed by N2 (T1 18, T2 21, T3 21), N1 (T1 17, T2 20, T3 20) and N0 (T1 16, T2 17, T3 18). During both T1 and T2, neutral detergent fibre content decreased by 4 percentage units and increased by a similar amount during T3. Generally, neutral detergent fibre content (%DM) was highest during T3 (53-58%), followed by T2 (45-54%) and T1 (43-49%). Water-soluble carbohydrate content (%DM) increased during all treatment periods with the highest level observed during T1 (18-31%) followed by T2 (3-14%) and T3 (1-6%). Nitrate content (measured as nitrate-nitrogen) decreased throughout T1, primarily due to dry conditions, while during T2, levels for N3 and N2 were significantly (P<0.05) higher than for N1 and N0 following nitrogen fertiliser application. During T3, nitrate content increased for all treatments throughout the 28-day period, with highest nitrate levels being observed during T3. The effect of applied nitrogen on mineral content was variable within and across treatment periods. The study indicates that nitrogen fertiliser did not affect metabolisable (apart from N3 elevating metabolisable energy during T3), neutral detergent fibre or water-soluble carbohydrate contents of perennial ryegrass during the 28 days after nitrogen application, but increased crude protein content. Also, nitrogen fertiliser elevated nitrate content in perennial ryegrass. While the elevated nitrate content observed may result in subclinical effects, these levels are not considered fatal for dairy cows. Crude protein content was generally above 20% of dry matter throughout the study and close to 30% of dry matter for short periods during T2. Minimising the effect of excess nitrogen ingested by the grazing animal may require appropriate supplementation of low crude protein containing feeds such as cereal grains. It is argued that the effects of rain and temperature, which impact on soil nitrogen mineralisation, may have a greater influence on perennial ryegrass nitrate content than nitrogen fertiliser.


1977 ◽  
Vol 57 (2) ◽  
pp. 255-261 ◽  
Author(s):  
L. J. FISHER ◽  
J. R. LESSARD

Wheat was harvested for silage at the milk, the soft dough and the firm dough stages of maturity and fed to lactating cows in a comparison with corn silage harvested at 30% dry matter. The protein content of the four silages ranged from 7 to 8.3%. The crude fiber content of the corn silage (22%) was lower than that of the three wheat silages ranging from 27.4% at the firm dough stage to 34.5% at the milk stage. Silage dry matter intake was highest for the firm dough wheat silage and lowest for the corn silage. However, there was no significant difference in milk yield or milk composition among the four silages. The higher intake of the firm dough silage compared to corn silage was compensated for by the tendency of higher digestibility of the corn silage. The rumen acetate content of the cows fed soft dough wheat silage was higher than for those fed corn silage and milk stage wheat silage, while the reverse was true for rumen propionate.


2021 ◽  
pp. 094-098
Author(s):  
Musa Tibin Musa Ahmed

This study was carried out to determine chemical composition and in vitro dry matter and organic matter digestibility of range plant species of the area. Samples were collected from range lands at full maturity stage, dried under shade and subjected to chemical analysis and dry matter and organic matter in vitro digestibility for Vigna sunhum, Andropogan gayanus, Pennisetum Pedicelatum, Shoenfeldia gracilis and Stylothanis flavicans were determined. The data was analyzed via analysis of variance and differences among means were determined using Least Significant Difference (LSD) test. The results showed that the legumes (Vigna sunhum and Stylothanis flavicans) were significantly higher (P≤0.01) in their crude protein content and the percentage of crude protein respectively was 13.4% and 10.00% while the grasses were significant (P≤0.05) higher in Crude Fibre (CF), ash, Acid Detergent Fibers (ADF) and Neutral Detergent Fibers (NDF). Crude fiber % values were 41.78, 43.37 and 46.07% for Pennisetum Pedicelatum, Andropogan gayanus and Shoenofeldia gracilis, respectively. The acid detergent values were 53.85, 47.74 and 53.39 for the three weeds. (P≤0.05), while the ratio of neutral detergent was significantly higher (P≤.05) in grasses than in the two legume species; 87.22, 82.35 and 88.99% for the three grasses respectively. Similarly higher in vitro dry matter and organic digestibility coefficients were obtained from legume species than grasses. The study concluded that, the studied grasses were higher in fiber content and lower in their protein content but, legumes grasses (Vigna sunhum, Pennisetum Pedicelatum and Stylothanis flavicans) had high nutritive value and in vitro digestibility which may provide ruminants with the needed protein and energy.


Author(s):  
Krystyna Zarzecka ◽  
Marek Gugała ◽  
Iwona Mystkowska ◽  
Krystyna Zarzecka ◽  
Anna Sikorska

1996 ◽  
Vol 24 (5) ◽  
pp. 715-725
Author(s):  
Katarina Ruppová ◽  
Darina Slameñová ◽  
Ladislave Wsølová ◽  
Alena Gábelová ◽  
Maria Vargová

The cytotoxic effects of short-term and long-term exposure of HeLa cells to paracetamol (acetaminophen) were assayed by total protein content reduction (microprotein assay) and by [14C]-L-leucine incorporation into macromolecular acid-insoluble cell fraction. The level of total protein content was followed over 72 hours and the level of [14C]-L-leucine incorporation over 24 hours, after paracetamol treatment. Statistical evaluation did not show a significant difference between results obtained by these two methods. In addition, the influence of S9 fraction on [14C]-L-leucine incorporation and the growth activity of paracetamol-treated HeLa cells were assayed. In these experiments, short-term paracetamol treatments (2 hours in phosphate buffered saline), were used. Statistical analysis of the data did not show an increase in paracetamol-induced cytotoxicity in the presence of the S9 fraction; on the contrary, a protective effect of S9 fraction on paracetamol-treated cells was found.


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