scholarly journals The influence of herbicides and their mixtures on total proteins content and on proper proteins in potato tubers

2011 ◽  
Vol 51 (No. 11) ◽  
pp. 517-522
Author(s):  
K. Zarzecka ◽  
M. Gugała
Keyword(s):  
Statistical Analysis ◽  
Protein Content ◽  
Total Protein ◽  
Field Experiments ◽  
Control Object ◽  
Weather Conditions ◽  
Total Protein Content ◽  
Potato Tubers ◽  
Total Proteins ◽  
Chemical Agents

Field experiments were carried out at the Agricultural Experimental Nuisance Station of University of Podlasie in Siedlce in the years 1999–2001. The influence of herbicides and their mixtures on total proteins content and on proper proteins in eatable potato tubers were analysed. The weeding chemical agents (pesticides) used in the potato treatment increased the total protein content by 0.09–0.18% and proper proteins content by 0.15–0.34% in the tubers in contrast with the ones of the control object. The statistical analysis also revealed the importance of the cultivar and weather conditions during the experimental years.

scholarly journals Total and True Protein Content in Potato Tubers Depending on Herbicides and Biostimulants

Agronomy ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1106
Author(s):  
Krystyna Zarzecka ◽  
Marek Gugała ◽  
Iwona Mystkowska ◽  
Anna Sikorska
Keyword(s):  
Field Experiment ◽  
Protein Content ◽  
Total Protein ◽  
Control Object ◽  
Potato Tubers ◽  
Ecklonia Maxima ◽  
Split Plot ◽  
Genetic Features ◽  
True Protein

The aim of the study was to determine the effect of herbicides and herbicides used with biostimulants on the content of total and true protein in potato tubers. The three-year field experiment was carried out using the random two-way split-plot arrangement in three repetitions. The examined factors were: I—three cultivars of potato: Bartek, Gawin, Honorata; II—five method applications of herbicides and herbicides with biostimulants: 1. Control—mechanical weeding, 2. Harrier 295 ZC (linuron + clomazone), 3. Harrier 295 ZC + Kelpak SL (linuron + clomazone and extract from algae Ecklonia maxima), 4. Sencor 70 WG (metribuzin), 5. Sencor 70 WG + Asahi SL (metribuzin and sodium p-nitrophenolate, sodium o-nitrophenolate, sodium 5-nitroguolacolate). On the plots sprayed with herbicides and herbicides mixed with biostimulants, a significant increase in the content of total and true proteins and the share of true protein in total protein in relation to tubers harvested from the control object was found. Genetic features of cultivars determined the content of total and true protein in potato tubers. The highest total and true protein was accumulated by tubers of the Bartek cultivar, and the least by the Honorata cultivar.

scholarly journals The effect of selected factors on the content of protein and nitrates in potato tubers

2011 ◽  
Vol 51 (No. 10) ◽  
pp. 431-438 ◽  
Author(s):  
J. Lachman ◽  
K. Hamouz ◽  
P. Dvořák ◽  
M. Orsák
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Dry Matter ◽  
Field Trials ◽  
Nitrate Content ◽  
Potato Tubers ◽  
Total Proteins ◽  
Lower Region ◽  
Significant Difference

The aim of the study was to determine the effect of region, variety, year and ecological and conventional way of cultivation on the content of total protein and nitrates in potato tubers in three-year field trials. The effects of selected factors on nitrate and protein contents were similar, more significant for nitrates. Growing regions, varieties and years of cultivation caused statistically significant effects whereas the effect of ecological way of cultivation was less pronounced and showed a tendency without significance. Higher region showed lesser average nitrate (113.4 mg/kg) and protein content (4.27% dry matter) in comparison with lower region (143.7 mg/kg and 4.72% dry matter). The highest nitrate content was found in Impala and Karin varieties (199.2 and 167.6 mg/kg); Karin variety was also rich in total proteins (4.86% dry matter). Ecological way of cultivation showed no significant difference on nitrate content (136.9 mg/kg) and protein content (4.41%) as compared with conventional way of cultivation (153.9 mg/kg, 4.32%).

scholarly journals Grain Yield and Total Protein Content of Organically Grown Oats–Vetch Mixtures Depending on Soil Type and Oats’ Cultivar

Agriculture ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 79
Author(s):  
Katarzyna Pużyńska ◽  
Stanisław Pużyński ◽  
Agnieszka Synowiec ◽  
Jan Bocianowski ◽  
Andrzej Lepiarczyk
Keyword(s):  
Grain Yield ◽  
Protein Content ◽  
Total Protein ◽  
Seed Mass ◽  
Weather Conditions ◽  
Total Protein Content ◽  
Common Vetch ◽  
Oat Cultivars ◽  
Set Up ◽  
Organically Grown

The yield and quality of crop mixtures depend on natural and agrotechnical factors and their relationships. This research aimed to analyze the grain yield, its components and total protein content of the organically grown oat–vetch mixture on two different soils and depending on the oat cultivar. The three-year field experiment with two crop rotations was carried out. The experiment was set up in the southern Poland on two soils: Stagnic Luvisol (S.L.) and Haplic Cambisol (H.C.). One of four oat cultivars (‘Celer’, ‘Furman’, ‘Grajcar’ and ‘Kasztan’) was grown with the common vetch cv. ‘Hanka’. The results showed that the grain yield of mixtures was affected mainly by weather conditions. During the dry season, the share of vetch in the grain yield was 46% lower than in the season of regular rainfall. The share of vetch seeds in the mixture’s yield was ca. 21% higher when the mixtures were grown on the S.L. than the H.C. soil. The selection of oats’ cultivar for the mixture with vetch affected significantly the thousand seed mass and protein content in the vetch seeds, 46.2–50.4 g and 270–280 g kg−1, respectively. The mixture with Kasztan cultivar yielded the best and this oat cultivar seemed to be the most appropriate for organic conditions; however, in years with high variability of rainfall distribution its usefulness was less.

Prediction of Human Acute Toxicity by the Hep G2/24-hour/Total Protein Assay, with Protein Measurement by the CBQCA Method

2005 ◽  
Vol 33 (3) ◽  
pp. 207-213 ◽  
Author(s):  
Paul J. Dierickx
Keyword(s):  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Human Toxicity ◽  
Hep G2 ◽  
Vitro Assay ◽  
Protein Assay ◽  
Lowry Method ◽  
Total Protein Assay

In our previously described Hep G2/24-hour/total protein assay, protein levels were measured by using the Lowry method. This assay was the best acute in vitro assay for the prediction of human toxicity within the Multicentre Evaluation of In Vitro Cytotoxicity (MEIC) study. In order to increase the MEIC data-base with a wider range of chemicals, we were interested in introducing the more practical 3-(4-carboxybenzoyl)-quinoline-2-carboxaldehyde (CBQCA) method for the quantification of the total protein content. Therefore, we investigated whether the same good results for the prediction of acute human toxicity would be obtained with the CBQCA method. The cells were treated for 24 hours, then cytotoxicity was determined by measuring the total protein content with CBQCA. The results were quantified by using the PI50c: the concentration (in mM) of test compound required to reduce the total protein content measured with the CBQCA-method by 50% as compared to the control cells. The results were compared with the PI50, the corresponding value when the Lowry method was used. A relatively low correlation was observed between PI50 and PI50c, reflecting the large and unexpected, differences when using the two protein assays. However, when comparing the log PI50c with the human toxicity, a correlation coefficient of r2 = 0.761 ( n = 44) was obtained for exactly the same series of MEIC chemicals. This value is clearly higher than that for the Lowry method ( r2 = 0.695). Compared to the Lowry method originally used, the Hep G2/24-hour/CBQCA total protein assay has the additional important advantage that it can be very easily adapted for large-scale analyses with robotic systems, including the on-line calculation of the results.

Total protein content and protein synthesis within pre-elongation stage bovine embryos

1998 ◽  
Vol 50 (2) ◽  
pp. 139-145 ◽  
Author(s):  
J.G. Thompson ◽  
A.N.M. Sherman ◽  
N.W. Allen ◽  
L.T. McGowan ◽  
H.R. Tervit
Keyword(s):  
Protein Synthesis ◽  
Protein Content ◽  
Total Protein ◽  
Total Protein Content ◽  
Bovine Embryos

scholarly journals Surface topography of hydroxyapatite affects ROS17/2.8 cells response

2002 ◽  
Vol 16 (3) ◽  
pp. 209-215 ◽  
Author(s):  
Adalberto Luiz Rosa ◽  
Márcio Mateus Beloti ◽  
Richard van Noort ◽  
Paul Vincent Hatton ◽  
Anne Jane Devlin
Keyword(s):  
Cell Proliferation ◽  
Protein Content ◽  
Surface Topography ◽  
Cell Morphology ◽  
Total Protein ◽  
Cell Attachment ◽  
Maxillofacial Surgery ◽  
Total Protein Content ◽  
Alp Activity ◽  
Powder Pressing

Hydroxyapatite (HA) has been used in orthopedic, dental, and maxillofacial surgery as a bone substitute. The aim of this investigation was to study the effect of surface topography produced by the presence of microporosity on cell response, evaluating: cell attachment, cell morphology, cell proliferation, total protein content, and alkaline phosphatase (ALP) activity. HA discs with different percentages of microporosity (< 5%, 15%, and 30%) were confected by means of the combination of uniaxial powder pressing and different sintering conditions. ROS17/2.8 cells were cultured on HA discs. For the evaluation of attachment, cells were cultured for two hours. Cell morphology was evaluated after seven days. After seven and fourteen days, cell proliferation, total protein content, and ALP activity were measured. Data were compared by means of ANOVA and Duncan’s multiple range test, when appropriate. Cell attachment (p = 0.11) and total protein content (p = 0.31) were not affected by surface topography. Proliferation after 7 and 14 days (p = 0.0007 and p = 0.003, respectively), and ALP activity (p = 0.0007) were both significantly decreased by the most irregular surface (HA30). These results suggest that initial cell events were not affected by surface topography, while surfaces with more regular topography, as those present in HA with 15% or less of microporosity, favored intermediary and final events such as cell proliferation and ALP activity.

Development and regression of exercise-induced cardiac hypertrophy in rats

1979 ◽  
Vol 236 (2) ◽  
pp. H268-H272 ◽  
Author(s):  
R. C. Hickson ◽  
G. T. Hammons ◽  
J. O. Holoszy
Keyword(s):  
Cytochrome C ◽  
Protein Content ◽  
Total Protein ◽  
Female Rats ◽  
Heart Weight ◽  
Total Protein Content ◽  
Mitochondrial Marker ◽  
Time Intervals ◽  
Control Value ◽  
Exercise Induced

Adult female rats were exercised by daily swimming. All the increase in heart weight induced by the exercise occurred within 14 days and averaged 30%. The half times of the increases in heart weight and total protein content were about 4.5 days, whereas that of cytochrome c, which was used as a mitochondrial marker, was 6.5 days. The total amounts of DNA and of hydroxyproline in the heart, which were used to evaluate the degree of connective tissue hyperplasia, increased only slightly (8% and 10%, respectively). Other animals were subjected to the same swimming program for 21 days. Groups of rats were killed at various time intervals after stopping exercise. Heart weight, total protein content, and total cytochrome c content decreased rapidly initially, with 60% of the total regression of hypertrophy occurring during the first week. Thereafter, heart weight fell more gradually toward the sedentary control value. The hydroxyproline content of the heart, which was increased 10%, did not decrease during the regression of the hypertrophy.

scholarly journals Amino Acid Composition of Milk from Cow, Sheep and Goat Raised in Ailano and Valle Agricola, Two Localities of ‘Alto Casertano’ (Campania Region)

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2431
Author(s):  
Nicola Landi ◽  
Sara Ragucci ◽  
Antimo Di Maro
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Protein Content ◽  
Total Protein ◽  
Free Amino ◽  
Raw Milk ◽  
Total Amino Acid ◽  
Total Protein Content ◽  
Campania Region

Cow, sheep and goat raw milk raised in Ailano and Valle Agricola territories (‘Alto Casertano’, Italy) were characterized (raw proteins, free and total amino acids content) to assess milk quality. Raw milk with the highest total protein content is sheep milk followed by goat and cow milk from both localities. Total amino acid content in cow, goat and sheep raw milk is 4.58, 4.81 and 6.62 g per 100 g, respectively, in which the most abundant amino acid is glutamic acid (~20.36 g per 100 g of proteins). Vice versa, the free amino acids content characteristic profiles are different for each species. In particular, the most abundant free amino acid in cow, sheep and goat raw milk is glutamic acid (9.07 mg per 100 g), tyrosine (4.72 mg per 100 g) and glycine (4.54 mg per 100 g), respectively. In addition, goat raw milk is a source of taurine (14.92 mg per 100 g), retrieved in low amount in cow (1.38 mg per 100 g) and sheep (2.10 mg per 100 g) raw milk. Overall, raw milk from ‘Alto Casertano’ show a high total protein content and are a good source of essential amino acids.

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