scholarly journals Lysosomal proteolysis in skeletal muscles of bulls

2011 ◽  
Vol 49 (No. 8) ◽  
pp. 340-348
Author(s):  
S. J Rosochacki ◽  
T. Sakowski ◽  
J. Połoszynowicz ◽  
E. Juszczuk-Kubiak ◽  
A. Kowalik-Krupa ◽  
...  
Keyword(s):  
Cathepsin D ◽  
Skeletal Muscles ◽  
General Linear Model ◽  
Black And White ◽  
Lysosomal Proteolysis ◽  
Longissimus Lumborum ◽  
Ad Libitum ◽  
The Relationship ◽  
Thiol Proteinases ◽  
Thiol Proteinase

The relationship between lysosomal proteolytic enzyme activities involved in skeletal muscle proteolysis of the longissimus lumborum et thoracis muscle (MLLT) of bulls was described. Samples from the same region were obtained post mortem from 7 Piemontese (P) and 54 Black-and-White bulls (B-W) about 18 months old fed ad libitum. The activity of cathepsin D was determined as pepstatin (cathepsin D inhibitor) sensitive activity (PSCatD) towards 1% haemoglobin. Pepstatin-insensitive acid (PIA) and leupeptin-insensitive (thiol proteinase inhibitor) acid (LIA) autolytic activities were measured in the presence of 1 mM Mg<sup>++</sup>. MLLT was also analysed for RNA, DNA and protein content. The data were processed by analysis of variance and differences between sires were tested by the contrast procedure of general linear model. In the examined muscle RNA decreased by 16% in B-W compared to P, CPS by about 14% and FCS by about 39%. DNA content was higher by 64.5% in B-W compared to P bulls (P&nbsp;&le; 0.01). Some differences were found between P bulls and B-W groups of sires in the percentage of proteins (P &le; 0.01), CatD and PSCatD (P &le; 0.01), but the most pronounced differences were determined in PIA and LIA (P &le; 0.01), and in the percentage of inhibition by pepstatin and leupeptin (P &le; 0.01) in AAA. In the Black-and-White group of sires the percentage of protein and percentage of inhibition by pepstatin and leupeptin in AAA were lowered by about 10, 17 and 22%, but PSCatD, PIA and LIA were higher by about 23.7, 41 and 57.7%, respectively, compared to Piemontese bulls. The level of aspartic and thiol proteinases was lower in the muscles of B-W compared to Piemontese. The activity was much higher in B-W compared to P. These results indicate the faster turnover of proteins in the groups after Black-and-White sires and higher anabolic increase in degradation in Piemontese bulls. &nbsp; &nbsp;

scholarly journals Cathepsin involvement in muscle proteolysis in meat-type bulls

2011 ◽  
Vol 50 (No. 9) ◽  
pp. 422-429
Author(s):  
Rosochacki SJ ◽  
T. Sakowski ◽  
E. Juszczuk-Kubiak ◽  
A. Butarewicz ◽  
J. Połoszynowicz
Keyword(s):  
Skeletal Muscle ◽  
Analysis Of Variance ◽  
Enzyme Activities ◽  
Cathepsin D ◽  
Proteolytic Enzyme ◽  
Complete Diet ◽  
Longissimus Lumborum ◽  
Ad Libitum ◽  
Thiol Proteinases ◽  
Meat Type

Measurements were done of some lysosomal proteolytic enzyme activities involved in skeletal muscle proteolysis of the masculus longissimus lumborum et thoracis muscle (MLLT) of bulls. Samples from the same region between the 11th and 13th vertebra were taken after slaughter from Limousin (n = 10), Hereford (n = 10), Charolais (n = 10), Angus (n = 11) and Simmental (n = 11) bulls about 15 months old fed complete diet ad libitum. The activity of cathepsin D was determined as pepstatin (cathepsin D inhibitor) sensitive activity (PSCatD) towards 1% haemoglobin. Pepstatin-insensitive acid (PIA) and leupeptin-insensitive (thiol proteinases inhibitor) acid (LIA) autolytic activities were measured in the presence of 1mM Mg<sup>++</sup>. MLLT was also analysed for RNA, DNA and protein variables. The data were processed by analysis of variance. The highest activities in PSCatD (P &le; 0.05), AAA (P &le; 0.01) and LIA (P &le; 0.05) as well as percentage of inhibition by pepstatin in cathepsin D (P &le; 0.01) were estimated in Angus bulls, and the lowest in Limousin ones. These breeds differed in the above-mentioned activities by 20.3, 21.1, 31.1 and 13.1%, respectively. RNA/g of tissue was highest in Hereford and lowest in Limousin bulls (by about 15.3%, P&nbsp;&le; 0.01). Similar differences (14.3%) were between Charolais and Limousin (P &le; 0.01). CPS (10<sup>3 </sup>RNA/protein) was higher by 18.3% (P &le; 0.01) in Charolais compared to the value in Simmental bulls; similar differences were between Hereford and Simmental (16.4%, P &le; 0.01). The DNA concentration was highest in Hereford (by about 30%) compared to Charolais bulls. Protein/10<sup>3</sup>DNA ratio (mg/mg) &ndash; FCS &ndash; was higher by 33.4% in Charolais compared toHereford; RNA/DNA ratio was higher by 40.2% in Charolais compared toLimousin bulls. These results indicate the fast turnover of proteins in the groups of examined bulls and it can be concluded that in hypertrophic MLLT of bulls an anabolic decrease in degradation occurred. &nbsp;

A note on relationship of plasma thyroxine and triiodothyronine rate to body weight in growing male camels

1994 ◽  
Vol 59 (3) ◽  
pp. 460-462
Author(s):  
M. M. Alfuraiji ◽  
S. M. Basmaeil ◽  
M. H. Gamil
Keyword(s):  
Body Weight ◽  
Crude Protein ◽  
Plasma Concentrations ◽  
General Linear Model ◽  
Rhodes Grass ◽  
Grass Hay ◽  
Ad Libitum ◽  
Relationship Of ◽  
The Relationship ◽  
General Linear Model Procedure

This study was conducted to determine the relationship between plasma concentrations of thyroxine (T4), triiodothyronine (T3) and body weight in young Majaheem male camels. Eighteen camels aged 6 to 7 months were divided equally into three groups (Gl, G2 and G3). All groups were given concentrate pellets (187 g crude protein per kg) at the rate of 15 g/kg body weight daily. In addition, each group was given one of the following roughages: lucerne hay to Gl, rhodes grass hay to G2 and ammonia-treated wheat straw to G3. Roughages, salt and water were measured and given ad libitum. Every 2 weeks all camels were weighed and blood samples were collected. Data were statistically analysed using a general linear model procedure. There was a positive correlation between T4 concentration and body weight. T4 was higher in camels given either hay or straw compared with those given rhodes grass hay, while there were no differences among the three groups in T3 and in body weight.

scholarly journals Correlation between the cocaine- and amphetamine-regulated transcript in the pyloric section of the abomasum and fat deposition in bulls’ carcasses

2015 ◽  
Vol 84 (4) ◽  
pp. 337-342 ◽  
Author(s):  
Krzysztof Młynek ◽  
Izabela Janiuk ◽  
Adam Oler ◽  
Marcin Maj ◽  
Lidia Borkowska
Keyword(s):  
Subcutaneous Fat ◽  
Endocrine System ◽  
Fat Deposition ◽  
The Body ◽  
Black And White ◽  
Longissimus Lumborum ◽  
System Cell ◽  
The Mean ◽  
The Relationship ◽  
Perinephric Fat

The study was aimed at investigating the relationship between the number of cocaine- and amphetamine-regulated transcript (CART) structures present in the pyloric section of the abomasum and fat accumulation in carcasses of bulls – crosses of Polish Lowland Black-and-White cows mated with Limousin bulls. The bulls were slaughtered at the mean age of 629 days and body weight of 597 kg. The distribution of cocaine- and amphetamine-regulated transcript-immunoreactive in the pyloric section of abomasum was assessed on the basis of CART positive immunohistochemical reactions. Significant interdependence was found between the number of CART structures and carcass adiposity and the fat content of the longissimus lumborum muscle. The identified tendency for fat tissue increase in the carcasses was accompanied by a lower concentration of the structures. In the endocrine system cell the greatest number of immunopositive cells were found; ranging from 28.9 in animals more obese to 37.8 with the smallest amount of fat accumulated. The lowest numbers of immunopositive cells, ranging from 2.4 to 3.9 were noted in the submucous plexus layer. Slightly more structures were observed in nerve fibres and the myenteric plexus, at 2.9 and 4.7, respectively. The number of CART in the endocrine system was correlated with subcutaneous fat (-0.451) and marbling (-0.514). The amount of CART in these anatomical layers was significantly correlated with the thickness of subcutaneous fat (-0.541 and -0.636) and the weight of perinephric fat (-0.487 and -0.672). The results confirm that CART is an important neurotransmitter that participates in the regulation of fat deposition in the body. It must be stressed that the correlations with the analysed fat deposition indices concerned the adiposity indicators influencing the commercial value of carcasses and the meat traits important to the consumer.

TO STUDY THE DYNAMICS OF IMMUNITY IN BLACK-AND-WHITE COWS DEPENDING ON AGE

2020 ◽  
pp. 49-52
Author(s):  
М.В. ДОВЫДЕНКОВА
Keyword(s):  
Bactericidal Activity ◽  
Phagocytic Activity ◽  
Physiological State ◽  
Age Groups ◽  
Statistical Processing ◽  
Natural Resistance ◽  
Black And White ◽  
Feeding Methods ◽  
High Degree ◽  
The Relationship

Проведена статистическая обработка сформированной базы данных показателей неспецифической резистентности MS «Excel» с целью изучения динамики иммунитета по параметрам бактерицидной, лизоцимной и фагоцитарной активности у крупного рогатого скота черно-пестрой породы разных возрастов. Было установлено, что средние значения показателей лизоцимной и бактерицидной активности у телят в 6 мес были ниже (47,89% и 49,09%, соответственно), чем у животных в 3—4 года (53,75% и 52,81%). Выведено уравнение регрессии, описывающее взаимосвязь между средними показателями фагоцитарной активности и возрастом при высокой степени достоверности (R2=0,90). Изменения лизоцимной и бактерицидной активности в зависимости от возраста были недостоверными (R2=0,58 и R2=0,49, соответственно). Установлена высокая корреляция между бактерицидной и лизоцимной активностью. Отмечена динамика изменения неспецифического иммунитета у коров различных возрастных групп. Создание баз данных и постоянный мониторинг основных показателей естественной резистентности в зависимости от различных факторов (породы, возраста, стадий лактации, кормления, способов содержания), которые влияют на данные показатели, необходимо для оценки физиологического состояния организма животных и их устойчивости к болезням. Statistical processing of the generated MS "Excel" on indicators of non-specific resistance database was carried out with the aim of studying the dynamics of the development of immunity in terms of bactericidal, lysozyme and phagocytic activity in black-and-white cattle of different ages. It was found that the average values of indicators of lysozyme and bactericidal activity in calves at 6 months were lower (47.89% and 49.09%, respectively) than in animals at the age of 3—4 years (53.75% and 52.81%). The equation of regression was derived, it describes the relationship between the average indicators of phagocytic activity and the age with a high degree of reliability (R2=0.90). The changes in lysozyme and bactericidal activity depending on age were dubious (R2=0.58 and R2=0.49, respectively). The high correlation between bactericidal and lysozyme activity was established. It was noticed that the cows of different age groups experienced the dynamics of changes in nonspecific immunity. The creation of databases and constant monitoring of the main indicators of natural resistance, depending on various factors (breed, age, stages of lactation, feeding, methods of keeping) that affect these indicators, is necessary for assessing the physiological state of the animals’ body of and their resistance to diseases.

The relationship between meal frequency and body mass index in black and white adolescent girls: more is less

2007 ◽  
Vol 32 (1) ◽  
pp. 23-29 ◽  
Author(s):  
D L Franko ◽  
R H Striegel-Moore ◽  
D Thompson ◽  
S G Affenito ◽  
G B Schreiber ◽  
...  
Keyword(s):  
Body Mass Index ◽  
Body Mass ◽  
Adolescent Girls ◽  
Meal Frequency ◽  
Black And White ◽  
The Relationship

THE ENDURING SIGNIFICANCE OF RACIAL AND ETHNIC DISPARITIES IN MALE VIOLENT VICTIMIZATION, 1973–2010

2018 ◽  
Vol 15 (1) ◽  
pp. 69-87 ◽  
Author(s):  
Janet L. Lauritsen ◽  
Karen Heimer ◽  
Joseph B. Lang
Keyword(s):  
Race And Ethnicity ◽  
White Male ◽  
Ethnic Disparities ◽  
Violent Victimization ◽  
Crime Victimization ◽  
Individual Level ◽  
Black And White ◽  
White Males ◽  
Poverty Status ◽  
The Relationship

AbstractLatino and Black males are more likely to suffer serious violent victimization compared to White males, and it is likely that economic disadvantage and other individual level differences play a key role in these disparities. This study of self-reported data from the National Crime Victimization Survey (1973–2010) is the first effort to assess three important issues: 1) the extent to which the relationship between serious violent victimization and race and ethnicity can be accounted for by age, location of residence, poverty status, and employment; 2) whether these factors have similar influences among Black, White, and Latino males; and 3) whether the net risk for violence associated with race and ethnicity has diminished over time. Our results show that disparities between Black and White male violent victimization decrease approximately 70% once age, location of residence, poverty status, and employment are taken into account, and that differences between Latinos and White males are fully accounted for by these factors. Poverty status is the only factor that varies in the strength of its association with violence across groups. We also find little evidence to suggest that the association between race, ethnicity and victimization risk changed significantly from 1973 to 2010, once other factors are considered. Despite notable declines in violence over this time period, Black and White disparities in male victimization persist over the past four decades; however, the relationship between poverty status and violence has increased some for Black and White males.

The relationship between protein nutrition, reproductive effort and breakdown in immunity toTeladorsagia circumcinctain periparturient ewes

2001 ◽  
Vol 72 (3) ◽  
pp. 595-606 ◽  
Author(s):  
J.G. M. Houdijk ◽  
I. Kyriazakis ◽  
F. Jackson ◽  
R. L. Coop
Keyword(s):  
Host Resistance ◽  
Dry Matter ◽  
Reproductive Effort ◽  
Late Pregnancy ◽  
Gastrointestinal Nematodes ◽  
Dry Matter Intake ◽  
Teladorsagia Circumcincta ◽  
Nutrient Partitioning ◽  
Ad Libitum ◽  
The Relationship

AbstractA nutritional hypothesis for the occurrence of the periparturient relaxation of immunity (PPRI) to gastrointestinal nematodes was tested within the bounds of a nutrient partitioning framework. It was hypothesized that at times of a scarce supply of metabolizable protein (MP), an increase in MP supply or a reduction in MP demand (having singles instead of twins) should lower the extent of PPRI in sheep. Twenty-one single- and 21 twin-bearing and -rearing Finn-Dorset ewes, 2 to 7 years old, and repeatedly infected withTeladorsagia circumcincta, were given one of three pelleted foods (no. = 7). The foods were formulated to supply 0·8 (L), 1·0 (M) and 1·2 (H) times the MP requirements during lactation, and were offeredad libitumfrom d–21to d35(d0 is day of parturition). Faecal egg counts (FEC) were assessed twice weekly as an indicator for host resistance. Dry-matter intake (DMI) during late pregnancy was higher than expected, and MP supply did not limit performance at this stage. In accordance with the hypothesis, there were no indications of a breakdown of immunity toT. circumcinctaduring late pregnancy. DMI during lactation did not differ between the single- and twin-rearing ewes. Calculated milk production was lower for the L than for the M and H ewes, especially for the twin-rearing ewes. A temporarily elevated FEC was observed during the first few days in lactation, which was probably mainly due to changes in faecal output rather than to PPRI. The FEC returned to low, late pregnancy levels at d12and remained low for the single- but not for the twin-rearing ewes (P< 0·001). Overall, the L ewes had higher FEC than the M and H ewes from d19onwards; this effect was more pronounced for the twin- than for the single-rearing ewes. It was postulated that DMI achieved overcame MP scarcity and thus prevented breakdown in immunity in the single-rearing ewes. The data support the view that, at times of MP scarcity, an increase in MP supply and reduction in MP demand can lower the extent of the breakdown in immunity towardsT. circumcincta.

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