scholarly journals Induction of atopic dermatitis by epicutaneous sensitisation with Dermatophagoides farinae in nongenetically predisposed Beagle dogs

2021 ◽  
Vol 66 (No. 10) ◽  
pp. 413-422
Author(s):  
Y Jeong ◽  
T Yun ◽  
H Kim ◽  
Y Koo ◽  
JH Kang ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Environmental Exposure ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Specific Ige ◽  
Dermatophagoides Farinae ◽  
Beagle Dogs ◽  
Serum Samples ◽  
Skin Biopsies ◽  
Dermal Oedema

This study was performed to induce atopic dermatitis (AD) using nongenetically predisposed Beagle dogs. Five healthy Beagle dogs were used. Twice weekly for 12 weeks, the dogs were painted on the axillae and groin with a solution of Dermatophagoides farinae (D. farinae). Each dog was thereafter placed in a cage where a house dust mite (HDM) solution was applied on the bottom of the cage. The dog remained in the cage for 3 h daily for 3 consecutive days for the environmental exposure to HDM. Serum samples were collected at 0 week and 6 weeks after sensitisation, and at 0 h and 72 h after the environmental exposure. During the environmental exposure, skin biopsies were obtained at 0 h, 36 h, and 72 hours. After the first environmental exposure, no dog had any marked clinical sign. An additional sensitisation was subsequently administered for 10–13 weeks. Three of the five dogs developed pruritic dermatitis with skin lesions after the second exposure. The histopathology of the lesions revealed severe infiltration of inflammatory cells and dermal oedema. The levels of D. farinae-specific IgE were also elevated. This study demonstrated that AD could be induced by epicutaneous sensitisation with HDM in nongenetically predisposed dogs.

scholarly journals Gomisin M2 Ameliorates Atopic Dermatitis-like Skin Lesions via Inhibition of STAT1 and NF-κB Activation in 2,4-Dinitrochlorobenzene/Dermatophagoides farinae Extract-Induced BALB/c Mice

Molecules ◽  
2021 ◽  
Vol 26 (15) ◽  
pp. 4409
Author(s):  
Jinjoo Kang ◽  
Soyoung Lee ◽  
Namkyung Kim ◽  
Hima Dhakal ◽  
Taeg-Kyu Kwon ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Oral Administration ◽  
Skin Diseases ◽  
Nuclear Translocation ◽  
Skin Lesions ◽  
Biologically Active ◽  
Therapeutic Effects ◽  
Dermatophagoides Farinae ◽  
Tnf Α ◽  
Ifn Γ

The extracts of Schisandra chinensis (Turcz.) Baill. (Schisandraceae) have various therapeutic effects, including inflammation and allergy. In this study, gomisin M2 (GM2) was isolated from S. chinensis and its beneficial effects were assessed against atopic dermatitis (AD). We evaluated the therapeutic effects of GM2 on 2,4-dinitrochlorobenzene (DNCB) and Dermatophagoides farinae extract (DFE)-induced AD-like skin lesions with BALB/c mice ears and within the tumor necrosis factor (TNF)-α and interferon (IFN)-γ-stimulated keratinocytes. The oral administration of GM2 resulted in reduced epidermal and dermal thickness, infiltration of tissue eosinophils, mast cells, and helper T cells in AD-like lesions. GM2 suppressed the expression of IL-1β, IL-4, IL-5, IL-6, IL-12a, and TSLP in ear tissue and the expression of IFN-γ, IL-4, and IL-17A in auricular lymph nodes. GM2 also inhibited STAT1 and NF-κB phosphorylation in DNCB/DFE-induced AD-like lesions. The oral administration of GM2 reduced levels of IgE (DFE-specific and total) and IgG2a in the mice sera, as well as protein levels of IL-4, IL-6, and TSLP in ear tissues. In TNF-α/IFN-γ-stimulated keratinocytes, GM2 significantly inhibited IL-1β, IL-6, CXCL8, and CCL22 through the suppression of STAT1 phosphorylation and the nuclear translocation of NF-κB. Taken together, these results indicate that GM2 is a biologically active compound that exhibits inhibitory effects on skin inflammation and suggests that GM2 might serve as a remedy in inflammatory skin diseases, specifically on AD.

scholarly journals Chamaejasmine Isolated from Wikstroemia dolichantha Diels Suppresses 2,4-Dinitrofluoro-benzene-Induced Atopic Dermatitis in SKH-1 Hairless Mice

Biomolecules ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 697 ◽  
Author(s):  
Tae-Young Kim ◽  
No-June Park ◽  
Jonghwan Jegal ◽  
Sangho Choi ◽  
Sang Woo Lee ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Clinical Symptoms ◽  
Skin Barrier ◽  
Topical Administration ◽  
Skin Lesions ◽  
Specific Ige ◽  
Transepidermal Water Loss ◽  
Skin Barrier Function ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dermal Thickness

Plants of the genus Wikstroemia have long been used as traditional medicines to treat diseases like pneumonia, rheumatism, and bronchitis. This study was designed to determine the effect of chamaejasmine, a biflavonoid present in W. dolichantha, on atopic dermatitis (AD)-like skin lesions in a 2,4-dinitrochlorobenzene (DNCB)-induced murine model of AD. Initially, we examined the anti-allergic activities of ten flavonoids from W. dolichantha by measuring β-hexosaminidase release from RBL-2H3 cells. Subsequently, an SKH-1 hairless mouse model of AD was developed based on the topical application of DNCB. Chamaejasmine (0.5%) or pimecrolimus (1%, positive control) were applied to dorsal skins of DNCB-sensitized AD mice for two weeks. Serum IL-4 and IgE levels were determined using enzyme-linked immunosorbent assay kits and transepidermal water loss (TEWL) and skin hydration were measured using a Tewameter TM210 and a SKIN-O-MAT, respectively. Of the ten flavonoids isolated from W. dolichantha, chamaejasmine most potently inhibited DNP-specific IgE-induced degranulation in RBL-2H3 cells. Topical administration of chamaejasmine attenuated the clinical symptoms of DNCB-induced dermatitis (i.e., itching, dryness, erythema, and edema). Histological analyses demonstrated that dermal thickness and mast cell infiltration in dermis were significantly reduced by chamaejasmine. In addition, 0.5% chamaejasmine inhibited DNCB-induced increases in total IL-4 and IgE levels in serum, improved skin barrier function, and increased epidermis moisture. Our findings suggest chamaejasmine might be an effective therapeutic agent for the treatment of atopic diseases.

scholarly journals Fermented Morinda citrifolia (Noni) Alleviates DNCB-Induced Atopic Dermatitis in NC/Nga Mice through Modulating Immune Balance and Skin Barrier Function

Nutrients ◽  
2020 ◽  
Vol 12 (1) ◽  
pp. 249 ◽  
Author(s):  
Kim ◽  
Seong ◽  
Choung
Keyword(s):  
Atopic Dermatitis ◽  
Barrier Function ◽  
Molecular Mechanisms ◽  
Inflammatory Diseases ◽  
Skin Barrier ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Morinda Citrifolia ◽  
Skin Barrier Function ◽  
Immune Balance

Morinda citrifolia, a fruit generally known as “Noni”, has been traditionally used in parts of East Asia to relieve inflammatory diseases. Although several studies using noni have been reported, the effect of fermented Morinda citrifolia (F.NONI) on atopic dermatitis (AD) has not been investigated. Thus, we aimed to investigate the improving effect of F.NONI treatment on AD-like skin lesions and elucidate molecular mechanisms. F.NONI was prepared by the fermentation of noni fruit with probiotics and then extracted. F.NONI was orally administrated to NC/Nga mice to evaluate its therapeutic effect on 2,4-dinitrochlorobenzene (DNCB)-induced AD. Oral administration of F.NONI significantly alleviated AD lesions and symptoms such as dermatitis scores, ear thickness, scratching behavior, epidermal thickness, and infiltration of inflammatory cells (e.g., mast cells and eosinophils). In addition, F.NONI treatment reduced the levels of histamine, IgE and IgG1/IgG2a ratio, thymus and activation regulated chemokine (TARC), and thymic stromal lymphopoietin (TSLP) in serum and beneficially modulated the expressions of Th1, Th2, Th17, and Th22-mediated cytokines in lesioned skin and splenocytes. Furthermore, the expressions of the skin barrier-related proteins including filaggrin (FLG), loricrin (LOR), involucrin (IVL), zonula occludens-1 (ZO-1), and occludin (OCC) were restored by F.NONI treatment. Taken together, these results suggest that F.NONI could be a therapeutic agent to attenuate AD-like skin lesions through modulating the immune balance and skin barrier function.

Patch testing of experimentally sensitized beagle dogs: development of a model for skin lesions of atopic dermatitis

2006 ◽  
Vol 17 (2) ◽  
pp. 95-102 ◽  
Author(s):  
Thierry Olivry ◽  
Kristin B. Deangelo ◽  
Stanley M. Dunston ◽  
Katie B. Clarke ◽  
Catherine A. Mccall
Keyword(s):  
Atopic Dermatitis ◽  
Patch Testing ◽  
Skin Lesions ◽  
Beagle Dogs

scholarly journals Topical Application of Herbal Mixture Extract Inhibits Ovalbumin- or 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Soon Re Kim ◽  
Han-Seok Choi ◽  
Hye Sook Seo ◽  
Youn Kyung Choi ◽  
Yong Cheol Shin ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Topical Application ◽  
Immunoglobulin E ◽  
Elevated Serum ◽  
Ethanol Extract ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Serum Ige ◽  
Beneficial Effects ◽  
Blood Eosinophils

KM110329 is four traditional herbal medicine mixtures with anti-inflammatory properties. Atopic dermatitis (AD) is an inflammatory skin disease associated with enhanced T-helper2 (Th2) lymphocyte response to allergens that results in elevated serum eosinophil and Immunoglobulin E (IgE) levels and leukocyte infiltration in atopic skin sites. In this study, we investigated the effect of topical application of KM110329 ethanol extract on the ovalbumin (OVA) or 2,4-dinitrochlorobenzene- (DNCB-) induced AD mouse models. For that purpose, we observed the effects of KM110329 on blood eosinophils, skin mast cells, production of serum IgE, and expression of cytokine mRNA in the atopic dermatitis skin lesions of OVA allergen- or DNCB-treated BALB/c mice. KM110329 significantly reduced blood eosinophils cell numbers in OVA or DNCB-treated BALB/c mice. Histological analyses demonstrated decreased mast cell count as well as dermal infiltration by inflammatory cells. In the skin lesions, mRNA expression of interleukine (IL)-4, IL-13, and IL-17 was inhibited by KM110329. KM110329 also suppressed the production of serum IgE level in both the OVA- and DNCB-induced atopic dermatitis model. Taken together, our results showed that topical application of KM110329 extracts exerts beneficial effects in AD symptoms, suggesting that KM110329 might be a useful candidate for the treatment of AD.

scholarly journals High-Intensity Resistance Training Suppresses Exacerbation of Atopic Dermatitis in Mice

2020 ◽  
Vol 29 (4) ◽  
pp. 443-449
Author(s):  
Eun-Ju Choi ◽  
Ki Chan Cho ◽  
Jooyoung Kim
Keyword(s):  
Atopic Dermatitis ◽  
Lymph Node ◽  
Resistance Training ◽  
Mrna Expression ◽  
High Intensity ◽  
Histopathological Examination ◽  
Inflammatory Cells ◽  
Skin Lesions ◽  
Positive Effects ◽  
Draining Lymph Node

PURPOSE: Training generally promotes health and inhibits diseases. However, in patients with atopic dermatitis (AD), training and sweating are significant aggravating factors. This study examined the effect of high-intensity resistance training (HIRT) on AD-like skin lesions in mice.METHODS: Eight-week-old female BALB/c mice were divided into four groups (control, HIRT, AD-only, and AD+HIRT). The mice in the HIRT group performed vertical ladder climbing for four weeks.RESULTS: After four weeks of HIRT, histopathological examination revealed reduced epidermis/dermis and dermal infiltration of inflammatory cells in the mice ear tissue. Additionally, HIRT suppressed serum immunoglobulin (Ig) levels and mRNA expression of pathogenic cytokines in the ear tissue; further, it reduced the size and weight of the draining lymph node (dLN) and non-dLN (ndLN), and the pathogenic cytokine-related mRNA expression of CD4+T cells from dLNs and ndLNs. We thus observed a negative correlation between HIRT and AD symptoms in mice.CONCLUSIONS:The results show that HIRT exerts positive effects in patients with AD.

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