scholarly journals Effects of Moringa Leaf Extract and Moderate Intensity Exercise on Histopathological Appearance and Autophagy Gene Expression

2021 ◽  
Vol 6 (1) ◽  
pp. 10-19
Author(s):  
Julia Windi Gunadi ◽  
Danti Dja Jatnika ◽  
Stephanie Astrid ◽  
Teresa Lucretia ◽  
Hamidie Ronald Daniel Ray ◽  
...  
Keyword(s):  
Gene Expression ◽  
Rat Liver ◽  
Leaf Extract ◽  
Wistar Rat ◽  
Exercise Group ◽  
Moderate Intensity ◽  
Control Group ◽  
Moderate Intensity Exercise ◽  
Autophagy Gene ◽  
Moringa Leaf Extract

The prevention of liver disease could be conducted through preventive strategies, such as antioxidants and exercise. Moringa leaf extract has substances that could act as antioxidant, while exercise is also known protecting the liver from disease by changing hepatic metabolism and autophagy. This study aimed to investigate the effect of moringa leaf extract and moderate intensity exercise on histopathological appearance and autophagy gene expression of wistar rat liver. The method used in this study was animal experiment using 24 male wistar rats divided into 4 groups, including control group, moringa group, exercise group, and moringa + exercise group. Moringa leaf extract was given in low dose (5.7 mg/kgW) per oral, 5 times a week, for 4 weeks. Meanwhile, the 20 m/minute treadmill exercise was given for 30 minutes per day, 5 times a week, for 4 weeks. Results showed a significant change on histopathological scoring in exercise group (p=0.011) compared to control group. Increased autophagy gene expression was found in moringa + exercise group compared to control group (LC3 0.90 fold; p62 0.87 fold). In summary, this study presented that moderate intensity exercise induced changes on histopathological appearance of wistar rat liver that might be associated with physiological inflammation. Moringa, with its antioxidant properties, combined with increased autophagy might improve histopathological changes in moringa + exercise group.

EFFECT OF LOW-, MODERATE-, AND HIGH-INTENSITY EXERCISE ON ATRIAL NATRIURETIC PEPTIDE (ANP) AND SARCOPLASMIC/ENDOPLASMIC RETICULUM CALCIUM-ATPase 2a (SERCA2a) GENE EXPRESSION IN WISTAR RAT CARDIAC MUSCLE

2019 ◽  
Vol 1 (2) ◽  
pp. 38
Author(s):  
Averina Octaxena Aslani ◽  
Hanna Goenawan ◽  
Nova Sylviana
Keyword(s):  
Gene Expression ◽  
Cardiac Muscle ◽  
High Intensity ◽  
Exercise Group ◽  
High Intensity Exercise ◽  
Cardiac Biomarkers ◽  
Moderate Intensity ◽  
Control Group ◽  
Heart Health ◽  
Moderate Intensity Exercise

Exercise is one of the factors that affect cardiovascular health. Exercise has been proven to reduce the number of cardiovascular diseases. However, it is also known that doing intense exercise has an adverse effect on heart health which can increase the risk of cardiovascular disease. While doing exercise, there is compensation from the heart itself by producing substances to maintain the heart to remain functional, known as cardiac biomarkers, including ANP and SERCA2a. Thus far, the recommended intensity is moderate-intensity exercise, which is optimal for heart health, but this recommendation has not been supported by biomolecular study. Therefore, this research was conducted. This study was done using semi-quantitative analytic method and experimental study design. Total of 24 male wistar rats were divided randomly into 4 groups: 1 control group (0m/min) and 3 exercise groups (low-: 10m/min, moderate-: 20m/min, high- intensity exercise: 30m/min). All groups received 30 min/day running, 5x/week for 12 weeks. The rats were terminated under anaesthesia, cardiac muscle extraction was done from left ventricle, and gene expression of ANP and SERCA2a is studied. Statistical analysis was done using SPSS software with one-way ANOVA followed by post hoc comparisons if the p-values < 0.05. The expression of ANP increased but insignificant in low-, moderate-, and high-intensity exercise group of rat (0.8720.034 vs 0.9010.018 vs 0.9160.015 vs 0.9380.025; p=0.304). The expression of SERCA2a also increased insignificantly in low-, moderate-, and high-intensity exercise group of rat (1.0440.019 vs 1.0510.015 vs 1.0630.011 vs 1.0820.027; p=0.493). Different exercise intensity didnt have significant effect on ANP and SERCA2a expression in rat cardiac muscle.

scholarly journals EFFECT OF LOW-, MODERATE-, AND HIGH-INTENSITY EXERCISE ON ATRIAL NATRIURETIC PEPTIDE (ANP) AND SARCOPLASMIC/ENDOPLASMIC RETICULUM CALCIUM-ATPase 2a (SERCA2a) GENE EXPRESSION IN WISTAR RAT CARDIAC MUSCLE

2021 ◽  
Vol 2 (2) ◽  
pp. 59
Author(s):  
Averina Octaxena Aslani ◽  
Hanna Goenawan ◽  
Nova Sylviana
Keyword(s):  
Gene Expression ◽  
Cardiac Muscle ◽  
High Intensity ◽  
Exercise Group ◽  
High Intensity Exercise ◽  
Cardiac Biomarkers ◽  
Moderate Intensity ◽  
Control Group ◽  
Heart Health ◽  
Moderate Intensity Exercise

Exercise is one of the factors that affect cardiovascular health. Exercise has been proven to reduce the number of cardiovascular diseases. However, it is also known that doing intense exercise has an adverse effect on heart health which can increase the risk of cardiovascular disease. While doing exercise, there is compensation from the heart itself by producing substances to maintain the heart to remain functional, known as cardiac biomarkers, including ANP and SERCA2a. Thus far, the recommended intensity is moderate-intensity exercise, which is optimal for heart health, but this recommendation has not been supported by biomolecular study. Therefore, this research was conducted. This study was done using semi-quantitative analytic method and experimental study design. Total of 24 male wistar rats were divided randomly into 4 groups: 1 control group (0m/min) and 3 exercise groups (low-: 10m/min, moderate-: 20m/min, high- intensity exercise: 30m/min). All groups received 30 min/day running, 5x/week for 12 weeks. The rats were terminated under anaesthesia, cardiac muscle extraction was done from left ventricle, and gene expression of ANP and SERCA2a is studied. Statistical analysis was done using SPSS software with one-way ANOVA followed by post hoc comparisons if the p-values < 0.05. The expression of ANP increased but insignificant in low-, moderate-, and high-intensity exercise group of rat (0.872±0.034 vs 0.901±0.018 vs 0.916±0.015 vs 0.938±0.025; p=0.304). The expression of SERCA2a also increased insignificantly in low-, moderate-, and high-intensity exercise group of rat (1.044±0.019 vs 1.051±0.015 vs 1.063±0.011 vs 1.082±0.027; p=0.493). Different exercise intensity didn’t have significant effect on ANP and SERCA2a expression in rat cardiac muscle.

scholarly journals The Experimental Effects of Acute Exercise on Long-Term Emotional Memory

2018 ◽  
Vol 7 (12) ◽  
pp. 486 ◽  
Author(s):  
Breanna Wade ◽  
Paul Loprinzi
Keyword(s):  
Acute Exercise ◽  
Group Interaction ◽  
Emotional Memory ◽  
Exercise Group ◽  
Moderate Intensity ◽  
Control Group ◽  
Moderate Intensity Exercise ◽  
Main Effect ◽  
Randomized Controlled Experiment ◽  
Memory Recognition

Emerging work suggests that acute, moderate-intensity aerobic exercise may help to subserve episodic memory of neutral stimuli. Less investigated, however, is whether acute exercise is associated with enhanced memory recognition of emotional stimuli, which was the purpose of this experiment. A parallel-group randomized controlled experiment was employed. Participants (mean age = 20 yr) were randomized into an exercise (n = 17) or control group (n = 17). The exercise group engaged in a 15-min bout of moderate-intensity treadmill walking. Emotional memory recognition was assessed via images from the International Affective Picture System, including assessments of varying degrees of valence and arousal. Memory recognition was assessed at 1 day, 7 days, and 14 days post-memory encoding. We observed a significant main effect for time (F(2) = 104.2, p < 0.001, η2p = 0.77) and a significant main effect for valence–arousal classification (F(4) = 21.39, p < 0.001, η2p = 0.40), but there was no significant time by group interaction (F(2) = 1.09, p = 0.34, η2p = 0.03), classification by group interaction (F(4) = 0.12, p = 0.97, η2p = 0.01), time by classification interaction (F(8) = 1.78, p = 0.08, η2p = 0.05), or time by classification by group interaction (F(8) = 0.78, p = 0.62, η2p = 0.02). In conclusion, emotional memory recognition decreased over the 14-day follow-up period and this rate of memory decay was not altered by acute moderate-intensity exercise engagement. We discuss these findings in the context of exercise intensity and the temporal effects of exercise.

scholarly journals PO-098 Effect of HIIT on mitochondrial telomerase of skeletal muscle in aged rats

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Yiyi Liu ◽  
Hao Su ◽  
Zhongye Jiang ◽  
Tianhao Wen ◽  
Jia Shao
Keyword(s):  
Skeletal Muscle ◽  
Oxygen Uptake ◽  
Intervention Group ◽  
Exercise Group ◽  
Moderate Intensity ◽  
Control Group ◽  
Moderate Intensity Exercise ◽  
Aging Rats ◽  
Age Related ◽  
Significant Difference

Objective The HIIT and moderate-intensity exercise are two different exercise models among the public fitness. In recent years, HIIT become more and more popular, unfortunately, there is a tremendous lack of research being done effects of mitochondrial reverse transcriptase (TERT) on age-related degeneration of skeletal muscle by HIIT. The purpose of this study was to compare the HIIT group and moderate-intensity group, and research difference of telomerase expression and cardiopulmonary endurance between the exercise group and the quiet control group was discussed. Methods  fifty-nine male Wistar rats were divided into three groups at random: control group (Q=19), moderate-intensity intervention group (M=20), and HIIT intervention group (H=20). The rats in Q group did not any exercise, and the rats in M group developed the exercise with 60% VO2max intensity for 8 weeks. H group did a training program for an 8-week exercise with alternating 40%, 60%, and 80% VO2max intensities. The rats in the experimental group were exercised for 50 minutes every day and trained for 5 days per week. After the baseline value group was sampled, each group of rats was selected after the training reached the specified number of weeks (4 and 8 weeks), and the maximum oxygen uptake test was performed before the material was taken. Single factor analysis of variance were used to assess differences in VO2max, and expression of protein between conditions. Results It was found that H group VO2max was significantly higher than M group and Q group (P<0.05). At same time, the mTERT expression of the M group at the 4th week was significantly higher than that of the Q group (P<0.05). The mTERT expression in group H was significantly higher than that in group Q at week 8 (P<0.05).There was no significant difference between the H group and the Q group at 8th week (P<0.05). Conclusions 1. HIIT exercise lasting for 8 weeks can effectively inhibit the decrease of maximal oxygen uptake in aging rats compared with moderate exercise. 2. HIIT training for 8 weeks promotes the expression of mTERT; 3. The maintenance of VO2max in aging rats may be related to the enhancement of mitochondrial antioxidant function by HIIT-promoted TERT to mitochondrial translocation.

scholarly journals The Effect of Low and Moderate Exercise on Hyperuricemia: Protocol for a Randomized Controlled Study

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuning Hou ◽  
Renyan Ma ◽  
Song Gao ◽  
Keneilwe Kenny Kaudimba ◽  
Hongmei Yan ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Heart Rate ◽  
Exercise Group ◽  
Moderate Intensity ◽  
Controlled Study ◽  
Control Group ◽  
Maximum Heart Rate ◽  
Moderate Intensity Exercise ◽  
Low Intensity ◽  
Low Intensity Exercise

BackgroundHyperuricemia (HUA) is a metabolic disease by purine metabolism disorders. It is a risk factor for many chronic diseases, including diabetes, hypertension, and heart disease. Studies have shown that exercise can effectively reduce serum uric acid (SUA), but the optimal exercise dose, intensity, and mode of exercise for improving HUA have not been verified in clinical studies. Therefore, this study aims to explore the effect of different exercise intensities in improving SUA of patients with HUA.Methods and AnalysisA randomized, single-blind, parallel controlled trial will be conducted in this study. 186 HUA patients who meet the inclusion criteria will be randomly divided into a 1:1:1 ratio (1): control group (2), low-intensity exercise group (brisk walking, 57-63% maximum heart rate, 150 min/week, 12 months), and (3) moderate-intensity exercise group (jogging, 64-76% maximum heart rate, 150 min/week, 12 months). The three groups of subjects will receive the same health education and prohibition of high-purine diet during the intervention period. The primary outcomes will be SUA concentration, SUA concentration change (mg/dL), SUA change rate (%), and the proportion of HUA patients. Secondary outcomes will include anthropometric parameters (body weight, waist circumference, hip circumference, BMI); physiological indicators (blood pressure, grip, vital capacity, maximum oxygen); biochemical indicators (blood lipid, blood sugar, liver enzyme, creatinine, and blood urea nitrogen). Each group of patients will go through an assessment at baseline, 3rd, 6th, and 12th months.DiscussionThis study will evaluate the effect of 12-month low-intensity exercise and moderate-intensity exercise on HUA patients. We hypothesize that both low-intensity and moderate-intensity exercise would improve HUA as compared with no-exercise control, and that moderate-intensity exercise would be more effective than low-intensity exercise in improving HUA. These results can provide a basis for the current physical activity guidelines for HUA’s healthy lifestyle management.Ethics and DisseminationThis study has been approved by the Ethical Review Committee of the Shanghai University of Sport (approval number: 102772020RT005). Informed consent will be obtained from all participants or their guardians. The authors intend to submit the study findings to peer-reviewed journals or academic conferences to be published.Clinical Trial RegistrationChinese Clinical Trial Registry, identifier ChiCTR2100042643.

scholarly journals Exercise improves long-term social and behavioral rhythms in older adults: Did it play a role during the COVID-19 lockdown?

2021 ◽  
Author(s):  
Giulia Cossu ◽  
Cesar Ivan Abbile Gonzalez ◽  
Luigi Minerba ◽  
Roberto Demontis ◽  
Massimiliano Pau ◽  
...  
Keyword(s):  
Older Adults ◽  
Controlled Trial ◽  
Exercise Program ◽  
Exercise Group ◽  
Moderate Intensity ◽  
Control Group ◽  
Moderate Intensity Exercise ◽  
Behavioral Rhythms

The study aimed to verify whether exercise training in older adults can improve social behavioral rhythms (SBR) and if any modification is maintained over time. Older adults (n=120) from a previous randomized controlled trial, were randomly allocated to either a moderate-intensity exercise group or a control group. SBR was evaluated at t0, t26, and t48 weeks (during the COVID-19 lockdown), using the brief social rhythms scale (BSRS). Seventy-nine participants completed the follow-up (age 72.3±4.7, women 55.3%). An improvement in the BSRS score was found in the exercise group at 26 weeks (p=0.035) when the exercise program was concluded, and it was maintained at 48 weeks (p=0.013). No improvements were observed in the control group. To conclude, SBR, previously found as a resilience factor in older adults during COVID-19, appear to improve after a moderate 12 weeks exercise program, and the improvement persisted even after stopping exercise during the COVID-19 lockdown.

scholarly journals Effect of Exercise Duration on Klotho and GluN2B Gene Expressions in Hippocampus of Wistar Rats with Moderate Intensity Exercise

2020 ◽  
Vol 52 (3) ◽  
pp. 167-171
Author(s):  
Hanna Goenawan ◽  
Bela Ita Karina ◽  
Titing Nurhayati ◽  
Julia Windi Gunadi ◽  
Setiawan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wistar Rats ◽  
Exercise Duration ◽  
Exercise Group ◽  
Rat Hippocampus ◽  
Moderate Intensity ◽  
Gene Expressions ◽  
Moderate Intensity Exercise ◽  
Oxidative Damages ◽  
Animal Experimental Study

Exercise could delay the degeneration process by increasing Klotho, which is an endogen antioxidant. Klotho protects against oxidative damages, regulates hippocampal synaptic plasticity, and enhances GluN2B expression. This study aimed to analyze the effect of 14-day and 8-week moderate-intensity exercise on Klotho and GluN2B mRNA expressions in rat hippocampus. This study was an animal experimental study using Wistar rats that was performed from June to September 2019 at the Central Laboratory, Universitas Padjadjaran, Indonesia. Rats were divided into 4 groups: control (14-day control and 8-week control) and exercise group (14-day exercise and 8-week exercise). The exercise groups ran at a moderate intensity (20m/min) for 30min/day, 5x/week. Hippocampus was then subjected to klotho and GluN2B gene expression analysis. The exercise groups showed a higher Klotho expression, both in 14 days (0.844±0.124 vs. 1.057±0.013) and 8 weeks (0.897±0.072 vs. 1.380±0.168) although statistically insignificant (p=0.24). No difference in GluN2B gene expression was observed between control and exercise groups on 14 days (1±0.09 vs. 1.22±0.09) and 8 weeks (1.24±0.03 vs. 1.38±0.05; p=0.11). In conclusion, 14-day and 8-week moderate-intensity exercise did not alter Klotho and GluN2B expressions in rat hippocampus.

Abstract 19037: Exercise Reduces 24-hr Blood Pressure and Albuminuria in Patients with Moderate Diabetic Kidney Disease

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Ulf G Bronas ◽  
Marc Weber ◽  
Paul Drawz ◽  
John Bantle ◽  
Daniel Duprez ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Kidney Disease ◽  
Exercise Training ◽  
Exercise Intervention ◽  
Intervention Group ◽  
Exercise Group ◽  
Moderate Intensity ◽  
Control Group ◽  
Inverse Association ◽  
Moderate Intensity Exercise

Introduction: Observational studies have shown an inverse association between physical function and CVD mortality in patients with chronic kidney disease (CKD) through unknown mechanisms. We have previously reported a significant exercise-induced reduction in resting blood pressure (BP) in 85 patients with stage 2-4 type 2 diabetes related CKD (DKD). The efficacy of exercise to reduce 24-hour ambulatory blood pressure (ABP) and albumin-creatinine ratio (ACR) is unclear. The purpose of this study was to test the hypothesis that 12wks of exercise would reduce 24-hr ABP and ACR in patients with stage 3-4 DKD and hypertension, compared to the control group. Methods: We randomly assigned 28 participants (21 male, age 62.9 yrs, BMI 34.8 kg/m2) with stage 3-4 DKD and hypertension (141(17)/75 (10) mmHg) to either 12-wks of moderate-intensity exercise training, 4x/wk for 45 minutes (n=15) or a usual medical care control group (n=13). The primary endpoint was change in 24-hr ABP at 12-wks. Results: There were no significant differences between groups in baseline demographic or medical variables. There were no changes in pharmacological variables at 12-week follow-up; group comparisons were analyzed by ANCOVA using baseline and exercise performed as covariates. Systolic BP levels and ACR were significantly reduced in the exercise-intervention group compared to the control group (Table 1). Reductions in diastolic BP levels were not significant between groups. Heart rate variables did not differ between groups. There was a strong inverse association between change in aerobic capacity and change in systolic and diastolic BP levels in the exercise group only (r=-.56, p=.039, and -.66, p=.011). Conclusion: This study suggests that exercise training imparts a favorable modulation of BP levels and ACR in patients with stage 3-4 DKD, potentially providing insight into the cardioprotective effect of exercise in this population.

scholarly journals Acute Interval and Continuous Moderate Intensity Exercise Enhanced Circadian Thermogenic Activity through Browning related Genes in Obese Adolescent Female

2021 ◽  
Vol 17 (5) ◽  
pp. 566-581
Author(s):  
Sugiharto Sugiharto ◽  
Desiana Merawati ◽  
Adi Pranoto ◽  
Purwo Sri Rejeki ◽  
Moch. Nasmay Lupita ◽  
...  
Keyword(s):  
Exercise Group ◽  
Pharmacological Therapy ◽  
Moderate Intensity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Peroxisome Proliferator ◽  
Moderate Intensity Exercise ◽  
Oxidation Activity ◽  
Obese Adolescent ◽  
Significant Difference

Thermogenesis is associated with oxidation activity in muscle and fat tissue, the target of non-pharmacological therapy in preventing the increase in obesity. This research was designed to reveal the circadian profile of thermogenic gene expression after the acute interval and continuous moderate-intensity exercise. The subjects were 22 randomly selected obese adolescent females who met the predetermined inclusion criteria. The study subjects were then divided into three groups: control group (CG), acute interval moderate-intensity exercise group (AIMIE), and acute continuous moderate-intensity exercise group (ACMIE). Acute interval and continuous exercise were performed by running on a treadmill for 40-45 minutes, while moderate-intensity was defined as 60%-70% of the maximum heart rate (HRmax). The blood samples were collected initially (pre-exercise), followed by 10 minutes, 6 hours, and 24 hours post-acute interval and continuous moderate-intensity exercise treatment. Measurement of peroxisome proliferator-activated receptor-gamma coactivator 1 alpha (PGC-1α) and fibronectin type III domain 5 (FNDC-5) expressions in protein level were confirmed by enzyme-linked immunosorbent assay (ELISA) method. Data were analyzed using one way-ANOVA and two way-ANOVA with a significant level of 5%. The findings suggest a substantial increase in the expression of PGC-1α and FNDC-5 after exercise compared to before the workout. A significant difference in PGC-1α and FNDC-5 expressions between the control group compared to AIMIE and ACMIE (p ≤ 0.05) has been observed. However, there is no significant difference in PGC-1α and FNDC-5 expressions after exercise between AIMIE and ACMIE (p ≥ 0.05). In conclusion, acute interval and continuous moderate-intensity exercise increase the expression of thermogenesis-related genes. Hence, acute interval and continuous moderate-intensity exercise might be potential non-pharmacological therapy to prevent, reduce, and control the increasing prevalence of obesity.

scholarly journals Moderate-Intensity Exercise Improves Body Composition and Improves Physiological Markers of Stress in HIV-Infected Men

ISRN AIDS ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-14 ◽  
Author(s):  
Wesley David Dudgeon ◽  
Jason Reed Jaggers ◽  
Kenneth Doyle Phillips ◽  
John Larry Durstine ◽  
Stephanie E. Burgess ◽  
...  
Keyword(s):  
Body Composition ◽  
Exercise Training ◽  
Salivary Cortisol ◽  
Exercise Program ◽  
Exercise Group ◽  
Peak Strength ◽  
Moderate Intensity ◽  
Control Group ◽  
Moderate Intensity Exercise ◽  
Tissue Mass

HIV/AIDS and its treatment often alter body composition and result in poorer physical functioning. The aim of this study was to determine the effects of a moderate-intensity exercise program on body composition and the hormones and cytokines associated with adverse health outcomes. HIV-infected males (N=111) were randomized to an exercise group (EX) who completed 6 weeks of moderate-intensity exercise training, or to a nonintervention control group (CON). In pre- and postintervention, body composition was estimated via DXA, peak strength was assessed, and resting blood samples were obtained. There was a decrease in salivary cortisol at wake (P=0.025) in the EX and a trend (P=0.07) for a decrease 1 hour after waking. The EX had a significant increase in lean tissue mass (LTM) (P<0.001) following the intervention. Those in the EX below median body fat (20%) increased LTM (P=0.014) only, while those above 20% decreased fat mass (P=0.02), total fat (N=0.009), and trunk fat (P=0.001), while also increasing LTM (P=0.027). Peak strength increased between 14% and 28% on all exercises in the EX group. These data indicate that 6 weeks of moderate-intensity exercise training can decrease salivary cortisol levels, improve physical performance, and improve body composition in HIV-infected men.

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