Sex Identification in Cinereous Vulture (Aegypius monachus) from Feather and Blood Samples: A Case Report

2020 ◽  
Vol 37 (1) ◽  
pp. 50-52
Author(s):  
Seong Hoon Seok ◽  
Sun Young Kang ◽  
Jae Ik Han ◽  
Young Bin Im ◽  
Han Sang Yoo ◽  
...  
Keyword(s):  
Case Report ◽  
Sex Identification ◽  
Blood Samples ◽  
Cinereous Vulture

scholarly journals Flushing of an intravenous catheter

2019 ◽  
Vol 29 (3) ◽  
pp. 614-618
Author(s):  
Rutger C.C. Hengeveld ◽  
Bianca E. Olofsen ◽  
Edmée C. van Dongen-Lases ◽  
Peter A. Leenhouts ◽  
Victor F.H.A. Hakkenberg van Gaasbeek ◽  
...  
Keyword(s):  
Emergency Department ◽  
Renal Function ◽  
Case Report ◽  
Sodium Chloride ◽  
Continuous Infusion ◽  
Intravenous Catheter ◽  
Blood Samples ◽  
Close Proximity ◽  
Laboratory Results ◽  
Life Threatening

Introduction: Phlebotomy is an error-prone process in which mistakes are difficult to reveal. This case report describes the effect on laboratory results originating from a blood sample collected in close proximity to an intravenous catheter. Materials and methods: A 69-year-old male patient was referred to the Emergency department where pneumonia was suspected. Phlebotomy was performed to collect blood samples to assess electrolytes, renal function, liver function, infection and haematological parameters. Results: The laboratory analysis showed reduced potassium and calcium concentrations. To prevent life-threatening cardiac failure the clinician decided to correct those electrolytes. Remarkably, the electrocardiogram showed no abnormalities corresponding to hypokalaemia and hypocalcaemia. This observation, in combination with an overall increase in laboratory parameters with the exception of sodium and chloride, led to the suspicion of a preanalytical error. Retrospectively, an intravenous catheter was inserted in close proximity of the puncture place but no continuous infusion was started prior to phlebotomy. However, the intravenous catheter was flushed with sodium chloride. Since potential other causes were excluded, the flushing of the intravenous catheter with sodium chloride prior to phlebotomy was the most probable cause for the deviating laboratory results and subsequently for the unnecessary potassium and calcium suppletion. Conclusion: This case underlines the importance of caution in the interpretation of laboratory results obtained from specimens that are collected in the proximity of an intravenous catheter, even in the absence of continuous infusion.

scholarly journals A Case Report to Assess Passive Immunity in a COVID Positive Pregnant Patient

2020 ◽  
Vol 37 (12) ◽  
pp. 1280-1282
Author(s):  
Lorraine E. Toner ◽  
Shari E. Gelber ◽  
Juan A. Pena ◽  
Nathan S. Fox ◽  
Andrei Rebarber
Keyword(s):  
Case Report ◽  
Cord Blood ◽  
Pregnant Patient ◽  
Passive Immunity ◽  
Molecular Testing ◽  
Transplacental Passage ◽  
Blood Samples ◽  
Key Points ◽  
Fetal Cord Blood ◽  
Red Blood Cell Isoimmunization

Introduction Data regarding transplacental passage of maternal coronavirus disease 2019 (COVID-19) antibodies and potential immunity in the newborn is limited. Case Report We present a 25-year-old multigravida with known red blood cell isoimmunization, who was found to be COVID-19 positive at 27 weeks of gestation while undergoing serial periumbilical blood sampling and intrauterine transfusions. Maternal COVID-19 antibody was detected 2 weeks after positive molecular testing. Antibodies were never detected on cord blood samples from two intrauterine fetal cord blood samples as well as neonatal cord blood at the time of delivery. Conclusion This case demonstrates a lack of passive immunity of COVID-19 antibodies from a positive pregnant woman to her fetus, neither in utero nor at the time of birth. Further studies are needed to understand if passage of antibodies can occur and if that can confer passive immunity in the newborn. Key Points

scholarly journals Case Report of Necrotizing Fasciitis Associated withStreptococcus pneumoniae

2016 ◽  
Vol 2016 ◽  
pp. 1-4 ◽  
Author(s):  
Lei Jiao ◽  
Zain Chagla ◽  
Reham Mohammedsaeed Kaki ◽  
Gabriela Gohla ◽  
Marek Smieja
Keyword(s):  
Case Report ◽  
Streptococcus Pneumoniae ◽  
Necrotizing Fasciitis ◽  
Neutrophil Infiltration ◽  
Skin Lesions ◽  
Blood Samples ◽  
Surgical Exploration ◽  
Lower Back ◽  
Life Threatening ◽  
The Right

Necrotizing fasciitis, caused byStreptococcus pneumoniae, is an extremely rare and life-threatening bacterial soft tissue infection. We report a case of early necrotizing fasciitis associated withStreptococcus pneumoniaeinfection in a 26-year-old man who was immunocompromised with mixed connective tissue disease. The patient presented with acute, painful, erythematous, and edematous skin lesions of his right lower back, which rapidly progressed to the right knee. The patient underwent surgical exploration, and a diagnosis of necrotizing fasciitis was confirmed by pathological evidence of necrosis of the fascia and neutrophil infiltration in tissue biopsies. Cultures of fascial tissue biopsies and blood samples were positive forStreptococcus pneumoniae. To our knowledge, this is the first report of necrotizing fasciitis resulting fromStreptococcus pneumoniaediagnosed at early phase; the patient recovered well without surgical debridement.

scholarly journals Convulsive Seizures as Presenting Symptom of Metronidazole-Induced Encephalopathy: A Case Report

2018 ◽  
Vol 10 (1) ◽  
pp. 34-37 ◽  
Author(s):  
Caspar Godthaab Sørensen ◽  
William Kristian Karlsson ◽  
Faisal Mohammad Amin ◽  
Mette Lindelof
Keyword(s):  
Case Report ◽  
Rare Condition ◽  
Pleural Empyema ◽  
Clinical Suspicion ◽  
Cerebellar Dysfunction ◽  
Blood Samples ◽  
Hyperintense Signal ◽  
Signal Changes ◽  
Convulsive Seizures

Introduction: Encephalopathy and convulsive seizures are rare manifestations of metronidazole toxicity. The incidence is unknown, but the condition has most frequently been reported in patients in their fifth to sixth decades. Usually, this condition is regarded as reversible, but permanent deficits and even death have been reported. Case Report: A 66-year-old female patient undergoing metronidazole treatment for pleural empyema was admitted to our institution after her second episode of seizure. Over the course of 1 week after admittance, the patient developed several convulsive seizures along with progressive cerebellar dysfunction and cognitive impairment. MRI revealed bilateral, symmetrical hyperintense signal changes in the pons and dentate nuclei. EEG, ECG, lumbar puncture, and blood samples were normal. The patient improved already 2–3 days after discontinuation of metronidazole and was discharged fully recovered after 17 days. Follow-up clinical assessment and MRI were unremarkable. Conclusion: Metronidazole-induced encephalopathy is a rare condition, and due to a general lack of awareness the diagnosis is often delayed. This condition should be considered in metronidazole-treated patients presenting with unprovoked seizures, myoclonus, cerebellar signs, and encephalopathy. Characteristic MRI lesions may support the clinical suspicion.

scholarly journals Immunodeficiency in a Child with Rapadilino Syndrome: A Case Report and Review of the Literature

2015 ◽  
Vol 2015 ◽  
pp. 1-4
Author(s):  
M. M. G. Vollebregt ◽  
A. Malfroot ◽  
M. De Raedemaecker ◽  
M. van der Burg ◽  
J. E. van der Werff ten Bosch
Keyword(s):  
Case Report ◽  
T Cell ◽  
Interferon Gamma ◽  
Genetic Disease ◽  
Gamma Globulin ◽  
Treg Cells ◽  
Review Of The Literature ◽  
Blood Samples ◽  
Cell Responses ◽  
Work Up

Rapadilino syndrome is a genetic disease characterized by a characteristic clinical tableau. It is caused by mutations in RECQL4 gene. Immunodeficiency is not described as a classical feature of the disease. We present a 2-year-old girl with Rapadilino syndrome with important lymphadenopathies and pneumonia due to disseminatedMycobacterium lentiflavuminfection. An immunological work-up showed several unexpected abnormalities. Repeated blood samples showed severe lymphopenia. Immunophenotyping showed low T, B, and NK cells. No Treg cells were seen. T cell responses to stimulations were insufficient. The IL12/IL23 interferon gamma pathway was normal. Gamma globulin levels and vaccination responses were low. With this report, we aim to stress the importance of screening immunodeficiency in patients with RECQL4 mutations for immunodeficiency and the need to further research into its physiopathology.

scholarly journals Case report: Thrombosis in children

2020 ◽  
Vol 8 (5) ◽  
pp. 105-106
Author(s):  
Álvarez Endara Julio ◽  
 Susan Sardinas Castellón ◽  
Parra Nigañes Patricia ◽  
Arancibia Marianela ◽  
Nuñes Prado Natalia
Keyword(s):  
Case Report ◽  
Blood Transfusion ◽  
Air Transportation ◽  
Air Transport ◽  
Data Logger ◽  
Blood Samples ◽  
Vibration Data ◽  
Before And After ◽  
Temperature Controlled ◽  
Blood Transport

Objective: We examined the effects of passenger air transport by regular airplanes for air transportation on blood for transfusion. Materials and methods: Irradiated red blood cell (RBC) solutions were transported by regular passenger airplanes which and were static in the cabin during the flight. Blood samples were evaluated visually and biochemically before and after transport. Hemolysis of the transported samples was compared to that of the non-transported ones. The vibration in the airplanes was also measured using a vibration data logger. Results: There was no significant hemolysis of RBCs during airplane transport. The vibration in the airplane was lower than that in automobiles. The temperature in the active transport refrigerator (ATR) room was maintained at 2−6°C. There was no significant hemolysis of RBCs during the two transport routes. Conclusion: Temperature-controlled air transport of blood transport in the ATR 700 airplane maintained a the blood at a good quality that rendered blood it fit for blood transfusion.

scholarly journals Molecular bird sexing of sulphur‐crested cockatoo (Cacatua galerita) by poly

2021 ◽  
Vol 26 (1) ◽  
pp. 1
Author(s):  
Diana Savitri ◽  
Irhamna Putri ◽  
Warih Pulung Nugrahani ◽  
Medania Purwaningrum ◽  
Aris Haryanto
Keyword(s):  
Gene Segment ◽  
Bird Species ◽  
Sex Identification ◽  
Agarose Gel ◽  
Chain Reaction ◽  
Blood Samples ◽  
Pcr Product ◽  
In Captivity ◽  
Pcr Method ◽  
Polymerase Chain

Sex identification of endangered and protected birds in captivity is very important for conservation programs. Half of the world’s bird species are monomorphic, where male and female are difficult to distinguished morphologically, including cockatoos. Sex identification using molecular bird sexing is more accurate and applicable because it directly targets the sex chromosomes. The purpose of this study was to determine the sex of Sulphur‐crested cockatoo (Cacatua galerita) by detecting differences in the intron size of the chromodomain helicase DNA‐binding 1 (CHD1) gene on the Z and W chromosomes by polymerase chain reaction (PCR) method and to compare of plucked feathers and blood samples as DNA sources for molecular bird sexing. DNA was extracted from feather and blood samples from four C. galerita. Extracted DNA was amplified on the CHD1 gene by PCR method with P2, MP, and NP primers, which were visualized using agarose gel 1.5% under UV transilluminator with a wavelength of 280 nm. The resulting PCR product was detected at 392 bp for the CHD1 Z gene segment and 297 bp for CHD1 W gene segments, where males showed a single DNA band (ZZ) and females showed a double DNA band (ZW). Four C. galerita were 100% successfully determined, consisting of one female and three males. Electrophoresis results showed DNA bands from blood samples were thicker and brighter than DNA bands from feather samples.

scholarly journals BLOODSTREAM INFECTION WITH OLIGELLA UREOLYTICA IN INFANT: A CASE REPORT AND REVIEW OF LITERATURE

2020 ◽  
pp. 1-2
Author(s):  
APOORVA B ◽  
ZARINE KHAN ◽  
TARANA SARWAT
Keyword(s):  
Case Report ◽  
Respiratory Tract ◽  
Bloodstream Infection ◽  
Pure Culture ◽  
Pathogenic Bacteria ◽  
Human Pathogen ◽  
Review Of Literature ◽  
Blood Samples ◽  
Clinical Specimens

Oligella ureolytica is rarely involved as human pathogen but it is emerging as pathogenic bacteria. It is not commonly recovered from clinical specimens but when recovered, it is mostly from urinary and respiratory tract specimens as commensal organisms, but hardly from blood samples. We present here a case of O. ureolytica related bacteremia in a 1-month-old infant as a pure culture and we review the literature of previously reported cases of Oligella infections.

Molecular identification of sex in the monomorphic breed of pigeons

2019 ◽  
Author(s):  
J. Gruszczyñska ◽  
A. Alama ◽  
M. Mi¹sko ◽  
P. Florczuk - Ko³omyja ◽  
B. Grzegrzó³ka
Keyword(s):  
Molecular Identification ◽  
Protein Gene ◽  
Dna Binding Protein ◽  
Sex Identification ◽  
Z Chromosome ◽  
Agarose Gel ◽  
Blood Samples ◽  
Phenotypic Differences ◽  
Binding Protein Gene ◽  
The Difference

In many avian species, especially in monomorphic species and breeds, sex identification creates a serious problem, as they do not show any phenotypic differences. One of such breeds is the Wroclaw Meat Pigeon. In this study, molecular identification of sex with P2 and P8 primers used for the CHD1 (chromo-helicase-DNA-binding-protein) gene amplification was performed. Peripheral blood samples were analyzed from 46 birds, and their DNA was isolated with the phenol-chloroform method. The fragments (370 bp CHD1-Z; 350 bp CHD1-W) obtained from the PCR were cut with the BsuRI. Only the sequence in the Z chromosome was cut into fragments of 305 and 65 bp by the restriction enzyme. The difference between CHD1-Z and CHD1-W was visualized in 3% agarose gel. A single band was identified as male, whereas two bands (plus 1 invisible) were identified as female. Consequently, 23 specimens in each sex were identified.

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